AlfalfaGEDB | MsG0380013449.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013449.01

KEGG: -
GO: -
PFAM: PF14009

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013449.01.T01	XP_013459517.1	87.387	222	15	2	1	209	1	222	5.79E-133	382
MsG0380013449.01.T02	XP_013459517.1	92.793	222	13	2	1	219	1	222	7.90E-145	413

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013449.01.T01	A0A072UWV1	87.387	222	15	2	1	209	1	222	2.77e-133	382
MsG0380013449.01.T02	A0A072UWV1	92.793	222	13	2	1	219	1	222	3.77e-145	413

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048036.01	MsG0380013449.01	0.811871	5.635517e-51	3.528573e-48
MsG0080048345.01	MsG0380013449.01	0.857696	1.369205e-62	3.408872e-59
MsG0080048347.01	MsG0380013449.01	0.858771	6.551157e-63	1.690962e-59
MsG0080048348.01	MsG0380013449.01	0.845744	3.355942e-59	5.648636e-56
MsG0080048349.01	MsG0380013449.01	0.827613	1.420241e-54	1.377933e-51
MsG0080048350.01	MsG0380013449.01	0.833236	5.989501e-56	6.855956e-53
MsG0080048352.01	MsG0380013449.01	0.862482	4.912274e-64	1.440973e-60
MsG0180000234.01	MsG0380013449.01	0.816222	6.181293e-52	4.354264e-49
MsG0180000930.01	MsG0380013449.01	0.805512	1.288288e-49	6.828372e-47
MsG0180001050.01	MsG0380013449.01	0.847277	1.280252e-59	2.263767e-56

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013449.01.T01	MTR_3g045790	87.387	222	15	2	1	209	1	222	7.02e-137	382
MsG0380013449.01.T01	MTR_5g089820	65.128	195	64	1	1	195	1	191	2.07e-86	254
MsG0380013449.01.T01	MTR_4g118680	82.500	40	7	0	156	195	6	45	3.62e-15	67.8
MsG0380013449.01.T02	MTR_3g045790	92.793	222	13	2	1	219	1	222	9.56e-149	413
MsG0380013449.01.T02	MTR_5g089820	62.927	205	62	3	1	205	1	191	7.38e-85	251
MsG0380013449.01.T02	MTR_4g118680	82.500	40	7	0	166	205	6	45	4.03e-15	68.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013449.01.T01	AT5G12340	41.827	208	112	4	1	199	4	211	2.72e-48	157
MsG0380013449.01.T01	AT5G12340	41.827	208	112	4	1	199	1	208	2.81e-48	157
MsG0380013449.01.T01	AT1G28190	27.692	195	128	5	9	193	25	216	1.00e-13	68.6
MsG0380013449.01.T02	AT5G12340	44.385	187	94	5	33	209	25	211	1.29e-45	151
MsG0380013449.01.T02	AT5G12340	44.385	187	94	5	33	209	22	208	1.47e-45	151
MsG0380013449.01.T02	AT1G28190	26.601	203	130	5	9	203	25	216	1.16e-12	65.9

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Find 35 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGGTTAGACTTAGAA+AGG	0.491346	3:-38263230	MsG0380013449.01.T02:CDS
AACAAGTCAACAATCAAATT+TGG	0.260285	3:-38263478	MsG0380013449.01.T02:CDS
AATATAAGCTTAGCTGACGA+TGG	0.640456	3:+38263748	None:intergenic
AATCCTTCAAATACTCAAAA+GGG	0.496327	3:+38263449	None:intergenic
ACAAGCCTTGTGATGAACTC+TGG	0.550575	3:+38263397	None:intergenic
AGAAAGGAGAGAAAGAGAAA+AGG	0.528227	3:-38263214	MsG0380013449.01.T02:CDS
CAATCCTTCAAATACTCAAA+AGG	0.411864	3:+38263448	None:intergenic
CGGACACCCGATCCCTAGTT+TGG	0.310287	3:-38263623	MsG0380013449.01.T02:CDS
CGTCAGCTAAGCTTATATTT+TGG	0.226292	3:-38263744	MsG0380013449.01.T02:CDS
CGTCTTCTAAATCCAAACTA+GGG	0.587535	3:+38263611	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAAGGTTAGACTTAGAA+AGG	-	Chr3:38263788-38263807	MsG0380013449.01.T02:CDS	30.0%
CAATCCTTCAAATACTCAAA+AGG	+	Chr3:38263573-38263592	None:intergenic	30.0%
TCCAAAATAGTTTCAAGCTT+AGG	+	Chr3:38263768-38263787	None:intergenic	30.0%
TGCATGAAAAGAGATCAATA+GGG	+	Chr3:38263464-38263483	None:intergenic	30.0%
TTGCATGAAAAGAGATCAAT+AGG	+	Chr3:38263465-38263484	None:intergenic	30.0%
TTGTTAATGGAGATAGATCA+GGG	-	Chr3:38263640-38263659	MsG0380013449.01.T02:CDS	30.0%
AATATAAGCTTAGCTGACGA+TGG	+	Chr3:38263273-38263292	None:intergenic	35.0%
AGAAAGGAGAGAAAGAGAAA+AGG	-	Chr3:38263804-38263823	MsG0380013449.01.T02:CDS	35.0%
CGTCTTCTAAATCCAAACTA+GGG	+	Chr3:38263410-38263429	None:intergenic	35.0%
CTTGTTAATGGAGATAGATC+AGG	-	Chr3:38263639-38263658	MsG0380013449.01.T02:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	38263189	38263851	38263189	ID=MsG0380013449.01;Name=MsG0380013449.01
Chr3	mRNA	38263189	38263851	38263189	ID=MsG0380013449.01.T01;Parent=MsG0380013449.01;Name=MsG0380013449.01.T01;_AED=0.40;_eAED=0.40;_QI=0\|0\|0\|1\|0\|0\|2\|0\|210
Chr3	exon	38263189	38263760	38263189	ID=MsG0380013449.01.T01:exon:8407;Parent=MsG0380013449.01.T01
Chr3	exon	38263791	38263851	38263791	ID=MsG0380013449.01.T01:exon:8406;Parent=MsG0380013449.01.T01
Chr3	CDS	38263791	38263851	38263791	ID=MsG0380013449.01.T01:cds;Parent=MsG0380013449.01.T01
Chr3	CDS	38263189	38263760	38263189	ID=MsG0380013449.01.T01:cds;Parent=MsG0380013449.01.T01
Chr3	mRNA	38263189	38263851	38263189	ID=MsG0380013449.01.T02;Parent=MsG0380013449.01;Name=MsG0380013449.01.T02;_AED=0.39;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|220
Chr3	exon	38263189	38263851	38263189	ID=MsG0380013449.01.T02:exon:8408;Parent=MsG0380013449.01.T02
Chr3	CDS	38263189	38263851	38263189	ID=MsG0380013449.01.T02:cds;Parent=MsG0380013449.01.T02

Gene Sequence

>MsG0380013449.01.T02

ATGGGAAATTTAGTAGTGACTCCATGTTTTCATCCTCAAAACAGAAAATTATCATCTTCATCGTCGTCGTCGTCGTTGCTGCCATCGTCAGCTAAGCTTATATTTTGGCAAGGTTCCACGAGATTGCTAAACGGTAAGCACATCGCTGGCGAGATCTTGTTCGAGTACCCAGAGACGATGGTTTGTCACGCTGACTCGTTTTTCATCGGACACCCGATCCCTAGTTTGGATTTAGAAGACGAACTCTTTCAAGGAGAGACTTATTTTGTTCTCCCTATTGATCTCTTTTCATGCAAAACACTCTCAGTTTCTTCACTCTTATCATTTAGATCACATAATAATGATAGTAACAACAAGTCAACAATCAAATTTGGAGAGTGCCCTTTTGAGTATTTGAAGGATTGTGATGGAAAAGTGTTGATAAAGGTTATGCCAGAGTTCATCACAAGGCTTGTTAATGGAGATAGATCAGGGAATGATTGTTGCATTAGTAATAAAAGTAGCTTCCTTTGTAGTACCCCTGAGTTGAAAAAACATTATGAAATGCTTGTGAAGTCTAAGGATCAGGTTTGGTCTCCTAAGCTTGAAACTATTTTGGAAAACAAGGTTAGACTTAGAAAGGAGAGAAAGAGAAAAGGCACAACCACAAACTGTGTCTGCTAG

>MsG0380013449.01.T01

ATGGGAAATTTAGTAGTGACTCCATGTTTTCATCCTCAAAACAGAAAATTATCATCTTCATCTAAGCTTATATTTTGGCAAGGTTCCACGAGATTGCTAAACGGTAAGCACATCGCTGGCGAGATCTTGTTCGAGTACCCAGAGACGATGGTTTGTCACGCTGACTCGTTTTTCATCGGACACCCGATCCCTAGTTTGGATTTAGAAGACGAACTCTTTCAAGGAGAGACTTATTTTGTTCTCCCTATTGATCTCTTTTCATGCAAAACACTCTCAGTTTCTTCACTCTTATCATTTAGATCACATAATAATGATAGTAACAACAAGTCAACAATCAAATTTGGAGAGTGCCCTTTTGAGTATTTGAAGGATTGTGATGGAAAAGTGTTGATAAAGGTTATGCCAGAGTTCATCACAAGGCTTGTTAATGGAGATAGATCAGGGAATGATTGTTGCATTAGTAATAAAAGTAGCTTCCTTTGTAGTACCCCTGAGTTGAAAAAACATTATGAAATGCTTGTGAAGTCTAAGGATCAGGTTTGGTCTCCTAAGCTTGAAACTATTTTGGAAAACAAGGTTAGACTTAGAAAGGAGAGAAAGAGAAAAGGCACAACCACAAACTGTGTCTGCTAG

Protein sequence

>MsG0380013449.01.T02

MGNLVVTPCFHPQNRKLSSSSSSSSLLPSSAKLIFWQGSTRLLNGKHIAGEILFEYPETMVCHADSFFIGHPIPSLDLEDELFQGETYFVLPIDLFSCKTLSVSSLLSFRSHNNDSNNKSTIKFGECPFEYLKDCDGKVLIKVMPEFITRLVNGDRSGNDCCISNKSSFLCSTPELKKHYEMLVKSKDQVWSPKLETILENKVRLRKERKRKGTTTNCVC*

>MsG0380013449.01.T01

MGNLVVTPCFHPQNRKLSSSSKLIFWQGSTRLLNGKHIAGEILFEYPETMVCHADSFFIGHPIPSLDLEDELFQGETYFVLPIDLFSCKTLSVSSLLSFRSHNNDSNNKSTIKFGECPFEYLKDCDGKVLIKVMPEFITRLVNGDRSGNDCCISNKSSFLCSTPELKKHYEMLVKSKDQVWSPKLETILENKVRLRKERKRKGTTTNCVC*