AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013452.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013452.01.T01 MTR_3g045790 88.426 216 12 2 1 203 2 217 1.26e-134 377
MsG0380013452.01.T01 MTR_5g089820 64.433 194 65 1 1 194 2 191 8.72e-85 250
MsG0380013452.01.T01 MTR_4g118680 82.500 40 7 0 155 194 6 45 3.74e-15 67.8
MsG0380013452.01.T02 MTR_3g045790 91.204 216 16 2 9 221 2 217 1.99e-135 379
MsG0380013452.01.T02 MTR_5g089820 62.255 204 63 3 9 212 2 191 8.06e-83 246
MsG0380013452.01.T02 MTR_4g118680 82.500 40 7 0 173 212 6 45 5.39e-15 67.8
MsG0380013452.01.T03 MTR_3g045790 91.204 216 16 2 1 213 2 217 8.71e-136 380
MsG0380013452.01.T03 MTR_5g089820 62.255 204 63 3 1 204 2 191 3.74e-83 246
MsG0380013452.01.T03 MTR_4g118680 82.500 40 7 0 165 204 6 45 4.12e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013452.01.T01 AT5G12340 41.546 207 112 4 1 198 2 208 7.61e-48 156
MsG0380013452.01.T01 AT5G12340 41.546 207 112 4 1 198 5 211 8.48e-48 156
MsG0380013452.01.T01 AT1G28190 27.692 195 128 5 8 192 25 216 1.41e-13 68.2
MsG0380013452.01.T02 AT5G12340 44.385 187 94 5 40 216 25 211 1.82e-45 151
MsG0380013452.01.T02 AT5G12340 44.385 187 94 5 40 216 22 208 1.86e-45 151
MsG0380013452.01.T02 AT1G28190 26.601 203 130 5 16 210 25 216 1.57e-12 65.5
MsG0380013452.01.T03 AT5G12340 44.385 187 94 5 32 208 22 208 1.17e-45 151
MsG0380013452.01.T03 AT5G12340 44.385 187 94 5 32 208 25 211 1.70e-45 151
MsG0380013452.01.T03 AT1G28190 26.601 203 130 5 8 202 25 216 1.36e-12 65.5

Find 35 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTAAGCTTGAAACTATTT+TGG 0.091162 3:+38330680 MsG0380013452.01.T03:CDS
CGTCAGCTAAGCTTATATTT+TGG 0.226292 3:+38330190 MsG0380013452.01.T03:CDS
AACAAGTCAACAATCAAATT+TGG 0.260285 3:+38330456 MsG0380013452.01.T03:CDS
TTGCATGAAAAGAGATCAAT+AGG 0.299018 3:-38330378 None:intergenic
CGGACACCCGATCCCTAGTT+TGG 0.310287 3:+38330311 MsG0380013452.01.T03:CDS
TTCATCACAAGGCTTGTTAA+TGG 0.314435 3:+38330543 MsG0380013452.01.T03:CDS
GCGTGACAAACCATCGTCTC+TGG 0.356966 3:-38330273 None:intergenic
TGAAATGCTTGTGAAGTCTA+AGG 0.382530 3:+38330644 MsG0380013452.01.T03:CDS
CTTGTTAATGGAGATAGATC+AGG 0.402456 3:+38330555 MsG0380013452.01.T03:CDS
CAATCCTTCAAATACTCAAA+AGG 0.411864 3:-38330486 None:intergenic
TCCAAAATAGTTTCAAGCTT+AGG 0.413585 3:-38330681 None:intergenic
TTAGAAGACGAACTCTTTCA+AGG 0.441583 3:+38330336 MsG0380013452.01.T03:CDS
GCTTGTGAAGTCTAAGGATC+AGG 0.441885 3:+38330650 MsG0380013452.01.T03:CDS
TATCTAGCAGACACAGTTTG+TGG 0.463540 3:-38330751 None:intergenic
TCGTCTTCTAAATCCAAACT+AGG 0.463904 3:-38330324 None:intergenic
TGATGGAAAAGTGTTGATAA+AGG 0.464603 3:+38330509 MsG0380013452.01.T03:CDS
AAGGAGAGAAAAGAGAGAAA+AGG 0.490465 3:+38330723 MsG0380013452.01.T03:CDS
AAACAAGGTTAGACTTAGAA+AGG 0.491346 3:+38330704 MsG0380013452.01.T03:CDS
CTAAATCCAAACTAGGGATC+GGG 0.491708 3:-38330317 None:intergenic
AATCCTTCAAATACTCAAAA+GGG 0.496327 3:-38330485 None:intergenic
TGAAGTCTAAGGATCAGGTT+TGG 0.505035 3:+38330655 MsG0380013452.01.T03:CDS
TCTAAATCCAAACTAGGGAT+CGG 0.527145 3:-38330318 None:intergenic
ACAAGCCTTGTGATGAACTC+TGG 0.550575 3:-38330537 None:intergenic
CGTGACAAACCATCGTCTCT+GGG 0.573900 3:-38330272 None:intergenic
CTAAACGGTAAGCACATCGC+TGG 0.579320 3:+38330231 MsG0380013452.01.T03:CDS
GGTTCCACGAGATTGCTAAA+CGG 0.584296 3:+38330216 MsG0380013452.01.T03:CDS
CGTCTTCTAAATCCAAACTA+GGG 0.587535 3:-38330323 None:intergenic
TTGTTAATGGAGATAGATCA+GGG 0.611228 3:+38330556 MsG0380013452.01.T03:CDS
TCAACTCAGGGGTACTACAA+AGG 0.612009 3:-38330611 None:intergenic
GAGTATTTGAAGGATTGTGA+TGG 0.613286 3:+38330492 MsG0380013452.01.T03:CDS
TGCATGAAAAGAGATCAATA+GGG 0.622739 3:-38330377 None:intergenic
CTTACCGTTTAGCAATCTCG+TGG 0.635041 3:-38330220 None:intergenic
AATATAAGCTTAGCTGACGA+TGG 0.640456 3:-38330186 None:intergenic
GTTCGAGTACCCAGAGACGA+TGG 0.655665 3:+38330263 MsG0380013452.01.T03:CDS
TTATGCCAGAGTTCATCACA+AGG 0.712497 3:+38330532 MsG0380013452.01.T03:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTTATTTTCAACAA+TGG + Chr3:38330087-38330106 MsG0380013452.01.T02:CDS 10.0%
!!! TTTATTTTATTTTCAACAAT+GGG + Chr3:38330088-38330107 MsG0380013452.01.T02:CDS 10.0%
! AACAAGTCAACAATCAAATT+TGG + Chr3:38330456-38330475 MsG0380013452.01.T03:CDS 25.0%
! AATCCTTCAAATACTCAAAA+GGG - Chr3:38330488-38330507 None:intergenic 25.0%
!! GATGATAATTTTCTGTTTTG+AGG - Chr3:38330141-38330160 None:intergenic 25.0%
!!! ATAATGTTTTTTCAACTCAG+GGG - Chr3:38330625-38330644 None:intergenic 25.0%
!!! CATAATGTTTTTTCAACTCA+GGG - Chr3:38330626-38330645 None:intergenic 25.0%
!!! TCATAATGTTTTTTCAACTC+AGG - Chr3:38330627-38330646 None:intergenic 25.0%
!!! TGAAACTATTTTGGAAAACA+AGG + Chr3:38330689-38330708 MsG0380013452.01.T03:CDS 25.0%
AAACAAGGTTAGACTTAGAA+AGG + Chr3:38330704-38330723 MsG0380013452.01.T03:CDS 30.0%
CAATCCTTCAAATACTCAAA+AGG - Chr3:38330489-38330508 None:intergenic 30.0%
TCCAAAATAGTTTCAAGCTT+AGG - Chr3:38330684-38330703 None:intergenic 30.0%
TGCATGAAAAGAGATCAATA+GGG - Chr3:38330380-38330399 None:intergenic 30.0%
TTGCATGAAAAGAGATCAAT+AGG - Chr3:38330381-38330400 None:intergenic 30.0%
TTGTTAATGGAGATAGATCA+GGG + Chr3:38330556-38330575 MsG0380013452.01.T03:CDS 30.0%
! TCCTAAGCTTGAAACTATTT+TGG + Chr3:38330680-38330699 MsG0380013452.01.T03:CDS 30.0%
!! TGATGGAAAAGTGTTGATAA+AGG + Chr3:38330509-38330528 MsG0380013452.01.T03:CDS 30.0%
AAGGAGAGAAAAGAGAGAAA+AGG + Chr3:38330723-38330742 MsG0380013452.01.T03:CDS 35.0%
AATATAAGCTTAGCTGACGA+TGG - Chr3:38330189-38330208 None:intergenic 35.0%
CGTCTTCTAAATCCAAACTA+GGG - Chr3:38330326-38330345 None:intergenic 35.0%
CTTGTTAATGGAGATAGATC+AGG + Chr3:38330555-38330574 MsG0380013452.01.T03:CDS 35.0%
GAGTATTTGAAGGATTGTGA+TGG + Chr3:38330492-38330511 MsG0380013452.01.T03:CDS 35.0%
TCGTCTTCTAAATCCAAACT+AGG - Chr3:38330327-38330346 None:intergenic 35.0%
TCTAAATCCAAACTAGGGAT+CGG - Chr3:38330321-38330340 None:intergenic 35.0%
TGAAATGCTTGTGAAGTCTA+AGG + Chr3:38330644-38330663 MsG0380013452.01.T03:CDS 35.0%
TTAGAAGACGAACTCTTTCA+AGG + Chr3:38330336-38330355 MsG0380013452.01.T03:CDS 35.0%
! CGTCAGCTAAGCTTATATTT+TGG + Chr3:38330190-38330209 MsG0380013452.01.T03:CDS 35.0%
!! TTCATCACAAGGCTTGTTAA+TGG + Chr3:38330543-38330562 MsG0380013452.01.T03:CDS 35.0%
!!! CTGTTTTGAGGATGAAAACA+TGG - Chr3:38330129-38330148 None:intergenic 35.0%
!!! GCTAAGCTTATATTTTGGCA+AGG + Chr3:38330195-38330214 MsG0380013452.01.T03:CDS 35.0%
CTAAATCCAAACTAGGGATC+GGG - Chr3:38330320-38330339 None:intergenic 40.0%
TGAAGTCTAAGGATCAGGTT+TGG + Chr3:38330655-38330674 MsG0380013452.01.T03:CDS 40.0%
TTATGCCAGAGTTCATCACA+AGG + Chr3:38330532-38330551 MsG0380013452.01.T03:CDS 40.0%
!! GTGCCCTTTTGAGTATTTGA+AGG + Chr3:38330482-38330501 MsG0380013452.01.T03:CDS 40.0%
CTTACCGTTTAGCAATCTCG+TGG - Chr3:38330223-38330242 None:intergenic 45.0%
GCTTGTGAAGTCTAAGGATC+AGG + Chr3:38330650-38330669 MsG0380013452.01.T03:CDS 45.0%
GGTTCCACGAGATTGCTAAA+CGG + Chr3:38330216-38330235 MsG0380013452.01.T03:CDS 45.0%
! ACAAGCCTTGTGATGAACTC+TGG - Chr3:38330540-38330559 None:intergenic 45.0%
!! TCAACTCAGGGGTACTACAA+AGG - Chr3:38330614-38330633 None:intergenic 45.0%
!!! CACGCTGACTCGTTTTTCAT+CGG + Chr3:38330291-38330310 MsG0380013452.01.T03:CDS 45.0%
CGTGACAAACCATCGTCTCT+GGG - Chr3:38330275-38330294 None:intergenic 50.0%
CTAAACGGTAAGCACATCGC+TGG + Chr3:38330231-38330250 MsG0380013452.01.T03:CDS 50.0%
GCGTGACAAACCATCGTCTC+TGG - Chr3:38330276-38330295 None:intergenic 55.0%
GTTCGAGTACCCAGAGACGA+TGG + Chr3:38330263-38330282 MsG0380013452.01.T03:CDS 55.0%
CGGACACCCGATCCCTAGTT+TGG + Chr3:38330311-38330330 MsG0380013452.01.T03:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 38330084 38330770 38330084 ID=MsG0380013452.01;Name=MsG0380013452.01
Chr3 mRNA 38330108 38330770 38330108 ID=MsG0380013452.01.T01;Parent=MsG0380013452.01;Name=MsG0380013452.01.T01;_AED=0.41;_eAED=0.43;_QI=0|0|0|1|0|0|2|0|210
Chr3 exon 38330108 38330165 38330108 ID=MsG0380013452.01.T01:exon:12507;Parent=MsG0380013452.01.T01
Chr3 exon 38330196 38330770 38330196 ID=MsG0380013452.01.T01:exon:12508;Parent=MsG0380013452.01.T01
Chr3 CDS 38330108 38330165 38330108 ID=MsG0380013452.01.T01:cds;Parent=MsG0380013452.01.T01
Chr3 CDS 38330196 38330770 38330196 ID=MsG0380013452.01.T01:cds;Parent=MsG0380013452.01.T01
Chr3 mRNA 38330084 38330770 38330084 ID=MsG0380013452.01.T02;Parent=MsG0380013452.01;Name=MsG0380013452.01.T02;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|228
Chr3 exon 38330084 38330770 38330084 ID=MsG0380013452.01.T02:exon:12509;Parent=MsG0380013452.01.T02
Chr3 CDS 38330084 38330770 38330084 ID=MsG0380013452.01.T02:cds;Parent=MsG0380013452.01.T02
Chr3 mRNA 38330108 38330770 38330108 ID=MsG0380013452.01.T03;Parent=MsG0380013452.01;Name=MsG0380013452.01.T03;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|220
Chr3 exon 38330108 38330770 38330108 ID=MsG0380013452.01.T03:exon:12510;Parent=MsG0380013452.01.T03
Chr3 CDS 38330108 38330770 38330108 ID=MsG0380013452.01.T03:cds;Parent=MsG0380013452.01.T03
Gene Sequence

>MsG0380013452.01.T01

GGGAAATTAGTAGTGACTCCATGTTTTCATCCTCAAAACAGAAAATTATCATCTTCATCTAAGCTTATATTTTGGCAAGGTTCCACGAGATTGCTAAACGGTAAGCACATCGCTGGCGAGATCTTGTTCGAGTACCCAGAGACGATGGTTTGTCACGCTGACTCGTTTTTCATCGGACACCCGATCCCTAGTTTGGATTTAGAAGACGAACTCTTTCAAGGAGAGACTTATTTTGTTCTCCCTATTGATCTCTTTTCATGCAAAACACTCTCAGTTTCTTCACTCTTATCATTTAGATCACATAATAATGATAGTAACAACAAGTCAACAATCAAATTTGGAGAGTGCCCTTTTGAGTATTTGAAGGATTGTGATGGAAAAGTGTTGATAAAGGTTATGCCAGAGTTCATCACAAGGCTTGTTAATGGAGATAGATCAGGGAATGATTGTTGCATTAGTAATAAAAGTAGCTTCCTTTGTAGTACCCCTGAGTTGAAAAAACATTATGAAATGCTTGTGAAGTCTAAGGATCAGGTTTGGTCTCCTAAGCTTGAAACTATTTTGGAAAACAAGGTTAGACTTAGAAAGGAGAGAAAAGAGAGAAAAGGCACAACCACAAACTGTGTCTGCTAG

>MsG0380013452.01.T02

TTGATTTATTTTATTTTCAACAATGGGAAATTAGTAGTGACTCCATGTTTTCATCCTCAAAACAGAAAATTATCATCTTCATCGTCGTCGTCGTCGTTGCTGCCATCGTCAGCTAAGCTTATATTTTGGCAAGGTTCCACGAGATTGCTAAACGGTAAGCACATCGCTGGCGAGATCTTGTTCGAGTACCCAGAGACGATGGTTTGTCACGCTGACTCGTTTTTCATCGGACACCCGATCCCTAGTTTGGATTTAGAAGACGAACTCTTTCAAGGAGAGACTTATTTTGTTCTCCCTATTGATCTCTTTTCATGCAAAACACTCTCAGTTTCTTCACTCTTATCATTTAGATCACATAATAATGATAGTAACAACAAGTCAACAATCAAATTTGGAGAGTGCCCTTTTGAGTATTTGAAGGATTGTGATGGAAAAGTGTTGATAAAGGTTATGCCAGAGTTCATCACAAGGCTTGTTAATGGAGATAGATCAGGGAATGATTGTTGCATTAGTAATAAAAGTAGCTTCCTTTGTAGTACCCCTGAGTTGAAAAAACATTATGAAATGCTTGTGAAGTCTAAGGATCAGGTTTGGTCTCCTAAGCTTGAAACTATTTTGGAAAACAAGGTTAGACTTAGAAAGGAGAGAAAAGAGAGAAAAGGCACAACCACAAACTGTGTCTGCTAG

>MsG0380013452.01.T03

GGGAAATTAGTAGTGACTCCATGTTTTCATCCTCAAAACAGAAAATTATCATCTTCATCGTCGTCGTCGTCGTTGCTGCCATCGTCAGCTAAGCTTATATTTTGGCAAGGTTCCACGAGATTGCTAAACGGTAAGCACATCGCTGGCGAGATCTTGTTCGAGTACCCAGAGACGATGGTTTGTCACGCTGACTCGTTTTTCATCGGACACCCGATCCCTAGTTTGGATTTAGAAGACGAACTCTTTCAAGGAGAGACTTATTTTGTTCTCCCTATTGATCTCTTTTCATGCAAAACACTCTCAGTTTCTTCACTCTTATCATTTAGATCACATAATAATGATAGTAACAACAAGTCAACAATCAAATTTGGAGAGTGCCCTTTTGAGTATTTGAAGGATTGTGATGGAAAAGTGTTGATAAAGGTTATGCCAGAGTTCATCACAAGGCTTGTTAATGGAGATAGATCAGGGAATGATTGTTGCATTAGTAATAAAAGTAGCTTCCTTTGTAGTACCCCTGAGTTGAAAAAACATTATGAAATGCTTGTGAAGTCTAAGGATCAGGTTTGGTCTCCTAAGCTTGAAACTATTTTGGAAAACAAGGTTAGACTTAGAAAGGAGAGAAAAGAGAGAAAAGGCACAACCACAAACTGTGTCTGCTAG

Protein sequence

>MsG0380013452.01.T01

GKLVVTPCFHPQNRKLSSSSKLIFWQGSTRLLNGKHIAGEILFEYPETMVCHADSFFIGHPIPSLDLEDELFQGETYFVLPIDLFSCKTLSVSSLLSFRSHNNDSNNKSTIKFGECPFEYLKDCDGKVLIKVMPEFITRLVNGDRSGNDCCISNKSSFLCSTPELKKHYEMLVKSKDQVWSPKLETILENKVRLRKERKERKGTTTNCVC*

>MsG0380013452.01.T02

LIYFIFNNGKLVVTPCFHPQNRKLSSSSSSSSLLPSSAKLIFWQGSTRLLNGKHIAGEILFEYPETMVCHADSFFIGHPIPSLDLEDELFQGETYFVLPIDLFSCKTLSVSSLLSFRSHNNDSNNKSTIKFGECPFEYLKDCDGKVLIKVMPEFITRLVNGDRSGNDCCISNKSSFLCSTPELKKHYEMLVKSKDQVWSPKLETILENKVRLRKERKERKGTTTNCVC*

>MsG0380013452.01.T03

GKLVVTPCFHPQNRKLSSSSSSSSLLPSSAKLIFWQGSTRLLNGKHIAGEILFEYPETMVCHADSFFIGHPIPSLDLEDELFQGETYFVLPIDLFSCKTLSVSSLLSFRSHNNDSNNKSTIKFGECPFEYLKDCDGKVLIKVMPEFITRLVNGDRSGNDCCISNKSSFLCSTPELKKHYEMLVKSKDQVWSPKLETILENKVRLRKERKERKGTTTNCVC*