AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031704.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031704.01.T01 MTR_6g032820 98.198 111 2 0 1 111 65 175 5.18e-71 218
MsG0680031704.01.T01 MTR_7g085390 79.630 108 21 1 1 108 65 171 9.72e-54 175
MsG0680031704.01.T01 MTR_4g112025 63.303 109 38 2 1 108 70 177 3.26e-36 129
MsG0680031704.01.T01 MTR_1g053320 62.385 109 39 2 1 108 69 176 4.43e-35 125
MsG0680031704.01.T01 MTR_8g092070 54.286 105 46 2 1 104 73 176 3.08e-32 119
MsG0680031704.01.T01 MTR_1g010120 56.881 109 45 2 1 108 65 172 3.96e-31 115
MsG0680031704.01.T01 MTR_4g124440 54.286 105 46 2 1 104 67 170 7.26e-29 108
MsG0680031704.01.T01 MTR_3g095050 51.429 105 47 4 1 104 73 174 1.55e-26 102
MsG0680031704.01.T01 MTR_2g027560 48.571 105 52 2 1 104 67 170 1.59e-24 97.1
MsG0680031704.01.T01 MTR_3g108600 52.273 88 40 2 1 87 70 156 8.16e-21 86.7
MsG0680031704.01.T01 MTR_3g031770 47.368 114 56 3 1 112 66 177 1.98e-20 85.5
MsG0680031704.01.T01 MTR_4g134280 62.963 54 20 0 51 104 2 55 1.61e-15 71.2
MsG0680031704.01.T01 MTR_2g034470 37.374 99 58 2 1 98 69 164 1.96e-11 59.7
MsG0680031704.01.T01 MTR_2g034470 37.374 99 58 2 1 98 34 129 2.09e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031704.01.T01 AT1G64760 77.778 108 23 1 1 108 64 170 5.02e-52 171
MsG0680031704.01.T01 AT1G64760 77.778 108 23 1 1 108 64 170 5.02e-52 171
MsG0680031704.01.T01 AT2G19440 77.778 108 23 1 1 108 60 166 1.18e-51 169
MsG0680031704.01.T01 AT2G19440 77.778 108 23 1 1 108 60 166 1.18e-51 169
MsG0680031704.01.T01 AT5G18220 68.519 108 31 1 1 108 69 173 9.09e-44 149
MsG0680031704.01.T01 AT3G04010 66.667 108 35 1 1 108 72 178 8.25e-43 146
MsG0680031704.01.T01 AT5G64790 59.048 105 41 2 1 104 67 170 3.76e-34 123
MsG0680031704.01.T01 AT5G20870 58.879 107 42 2 1 106 69 174 7.89e-33 120
MsG0680031704.01.T01 AT3G24330 54.128 109 47 3 1 108 78 184 2.06e-30 113
MsG0680031704.01.T01 AT5G58090 57.143 105 43 2 1 104 61 164 4.25e-30 112
MsG0680031704.01.T01 AT4G31140 52.381 105 48 2 1 104 66 169 4.43e-28 107
MsG0680031704.01.T01 AT4G17180 43.269 104 54 3 1 104 64 162 2.15e-19 82.8
MsG0680031704.01.T01 AT5G58480 42.857 105 59 1 1 104 65 169 9.91e-17 75.1

Find 51 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATAAACATCAGCATTTA+AGG 0.121944 6:+24950013 None:intergenic
CACCTAATCCAACTTGATTT+AGG 0.179677 6:+24950057 None:intergenic
TATCTTTGAGTGAAAATTAA+TGG 0.197627 6:-24952054 MsG0680031704.01.T01:five_prime_UTR
ATTTCAGCTAATTGATTATT+AGG 0.201538 6:+24951799 None:intergenic
GTTCATCAGCTGGAATATTT+AGG 0.257207 6:-24949969 MsG0680031704.01.T01:CDS
AACATTTGTACAACTGTATC+TGG 0.259099 6:+24951918 None:intergenic
TGTCATAAGTTCACTTAATC+AGG 0.268618 6:+24949946 None:intergenic
CTATTCTATGTTTGATTGAT+TGG 0.274287 6:+24952447 None:intergenic
ATGTATGTGGTGTGAAAATA+AGG 0.275216 6:+24952567 None:intergenic
GAAGTATGATAATCTAATTA+AGG 0.279119 6:+24952524 None:intergenic
ATCTTTGAGTGAAAATTAAT+GGG 0.281401 6:-24952053 MsG0680031704.01.T01:five_prime_UTR
AGCACTTCTATATTTGTATA+TGG 0.290955 6:+24952129 None:intergenic
ACCTAATCCAACTTGATTTA+GGG 0.313740 6:+24950058 None:intergenic
GGAAGGACTTGGTGTGAATT+GGG 0.317702 6:-24951968 MsG0680031704.01.T01:five_prime_UTR
TGGAAGGACTTGGTGTGAAT+TGG 0.327363 6:-24951969 MsG0680031704.01.T01:five_prime_UTR
TGGATTAGGTGACTCAATTA+AGG 0.336092 6:-24950045 MsG0680031704.01.T01:CDS
GTACAAATGTTGAAAGATAA+TGG 0.351290 6:-24951906 MsG0680031704.01.T01:five_prime_UTR
TATTCTATGTTTGATTGATT+GGG 0.368789 6:+24952448 None:intergenic
ACTCGTTATAACTTCAAATC+TGG 0.376451 6:-24951733 MsG0680031704.01.T01:CDS
TGAACAGCATTGTTTGAATC+TGG 0.381318 6:+24949987 None:intergenic
AAGTATGATAATCTAATTAA+GGG 0.381909 6:+24952525 None:intergenic
AAGAAGAAGAAAACTAGAAA+TGG 0.397862 6:+24952613 None:intergenic
AGTAAGAAGAAGCTAGGAAA+TGG 0.425624 6:+24952318 None:intergenic
ATGAGTGCACTTGGTGGGTC+AGG 0.429886 6:-24951844 MsG0680031704.01.T01:CDS
GCCCTAAATCAAGTTGGATT+AGG 0.441972 6:-24950059 MsG0680031704.01.T01:CDS
ATAGGAGTGAAATTTATTAG+TGG 0.469236 6:+24952251 None:intergenic
TTATGATCGTGCTTTGCAAT+GGG 0.469916 6:-24951770 MsG0680031704.01.T01:CDS
AAACAATGCTGTTCATCAGC+TGG 0.472127 6:-24949979 MsG0680031704.01.T01:CDS
TCATAAACATCAGCATTTAA+GGG 0.503342 6:+24950014 None:intergenic
ACAACTGTATCTGGTTTCAA+TGG 0.506632 6:+24951927 None:intergenic
CAAAATGCCCTAAATCAAGT+TGG 0.507329 6:-24950065 MsG0680031704.01.T01:CDS
TGATACAGGTATGTAGCAGT+TGG 0.511141 6:-24950158 MsG0680031704.01.T01:intron
ATTATGATCGTGCTTTGCAA+TGG 0.517702 6:-24951771 MsG0680031704.01.T01:CDS
TGAACAAGTAAGAAGAAGCT+AGG 0.523091 6:+24952312 None:intergenic
AATGTTCATTAGGGCAGGGA+AGG 0.547103 6:+24950088 None:intergenic
TGGGTCAGGTATTGAAGTAA+TGG 0.555721 6:-24951830 MsG0680031704.01.T01:CDS
CGTTATAACTTCAAATCTGG+TGG 0.565387 6:-24951730 MsG0680031704.01.T01:CDS
ACTTCTATATTTGTATATGG+TGG 0.568585 6:+24952132 None:intergenic
ATTCTATGTTTGATTGATTG+GGG 0.569960 6:+24952449 None:intergenic
TCTTTGAGTGAAAATTAATG+GGG 0.578888 6:-24952052 MsG0680031704.01.T01:five_prime_UTR
AAACTATGAGTGCACTTGGT+GGG 0.583255 6:-24951849 MsG0680031704.01.T01:exon
AAGAAGCTAGGAAATGGAGT+GGG 0.596925 6:+24952324 None:intergenic
TAATATATGAATTATGTATG+TGG 0.598076 6:+24952554 None:intergenic
TGTATGTGGTGTGAAAATAA+GGG 0.608924 6:+24952568 None:intergenic
GAAGAAGCTAGGAAATGGAG+TGG 0.619126 6:+24952323 None:intergenic
GAAGGACTTGGTGTGAATTG+GGG 0.620435 6:-24951967 MsG0680031704.01.T01:five_prime_UTR
GAAGAAACTATGAGTGCACT+TGG 0.642757 6:-24951853 MsG0680031704.01.T01:exon
TTTGAATGTTCATTAGGGCA+GGG 0.642889 6:+24950084 None:intergenic
ATCAGCATTTAAGGGTACTG+TGG 0.661565 6:+24950022 None:intergenic
GAAACTATGAGTGCACTTGG+TGG 0.667838 6:-24951850 MsG0680031704.01.T01:exon
ATGTGGTGTGAAAATAAGGG+AGG 0.718069 6:+24952571 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATATACTAGTTAATA+TGG - Chr6:24951161-24951180 MsG0680031704.01.T01:intron 10.0%
!! AAGTATGATAATCTAATTAA+GGG + Chr6:24950045-24950064 None:intergenic 15.0%
!! TAATATATGAATTATGTATG+TGG + Chr6:24950016-24950035 None:intergenic 15.0%
!! TTTGAACAATAAACTAAAAT+TGG + Chr6:24951443-24951462 None:intergenic 15.0%
!!! CTTTAGCTTAAAAAAAAAAA+CGG - Chr6:24952214-24952233 MsG0680031704.01.T01:five_prime_UTR 15.0%
!!! CTTTTTTTAATGTCAATTAT+TGG - Chr6:24951238-24951257 MsG0680031704.01.T01:intron 15.0%
!!! GTTTTTATTTTTAATTGTTG+TGG - Chr6:24950916-24950935 MsG0680031704.01.T01:intron 15.0%
!! AAACAAAACTAGATCTTAAT+TGG - Chr6:24951813-24951832 MsG0680031704.01.T01:CDS 20.0%
!! ATCTTTGAGTGAAAATTAAT+GGG - Chr6:24950514-24950533 MsG0680031704.01.T01:intron 20.0%
!! ATTTCAGCTAATTGATTATT+AGG + Chr6:24950771-24950790 None:intergenic 20.0%
!! CAAAAAATGTCAAAACTATT+AGG - Chr6:24951031-24951050 MsG0680031704.01.T01:intron 20.0%
!! GAAGTATGATAATCTAATTA+AGG + Chr6:24950046-24950065 None:intergenic 20.0%
!! GAATATGATTCTATAAACTA+AGG - Chr6:24951351-24951370 MsG0680031704.01.T01:intron 20.0%
!! TAAAACTGATAAGAAATGAT+CGG - Chr6:24951309-24951328 MsG0680031704.01.T01:intron 20.0%
!! TATCAATAATAGTGTAAAGA+TGG + Chr6:24952394-24952413 None:intergenic 20.0%
!! TATCTTTGAGTGAAAATTAA+TGG - Chr6:24950513-24950532 MsG0680031704.01.T01:intron 20.0%
!! TATTCTATGTTTGATTGATT+GGG + Chr6:24950122-24950141 None:intergenic 20.0%
!! TGTGTTTAGGAATAAATAAT+TGG + Chr6:24952359-24952378 None:intergenic 20.0%
!! TTGTTGTATGATTTCAAAAA+TGG + Chr6:24952442-24952461 None:intergenic 20.0%
!!! AGTTTTTGTTAGAATAGTTT+AGG - Chr6:24952073-24952092 MsG0680031704.01.T01:five_prime_UTR 20.0%
!!! CATATTTTAAGGTGAATAAA+CGG - Chr6:24951968-24951987 MsG0680031704.01.T01:five_prime_UTR 20.0%
!!! CGACTAAAATTCATATTTTA+AGG - Chr6:24951957-24951976 MsG0680031704.01.T01:five_prime_UTR 20.0%
!!! GATTCTTTTTCTTTTTTTCT+TGG - Chr6:24951740-24951759 MsG0680031704.01.T01:CDS 20.0%
!!! GTTTTTGTTAGAATAGTTTA+GGG - Chr6:24952074-24952093 MsG0680031704.01.T01:five_prime_UTR 20.0%
!!! TCTCTGCATTTTTTTTTTTA+TGG - Chr6:24951611-24951630 MsG0680031704.01.T01:intron 20.0%
!!! TTAACTTCTAGTATGTTTTT+TGG - Chr6:24951509-24951528 MsG0680031704.01.T01:intron 20.0%
!!! TTCATCATATTTTTTGTAAC+TGG - Chr6:24952007-24952026 MsG0680031704.01.T01:five_prime_UTR 20.0%
! AAAAAAAAAAACGGTGTGTT+GGG - Chr6:24952223-24952242 MsG0680031704.01.T01:five_prime_UTR 25.0%
! AAATGTCAAAACTATTAGGT+CGG - Chr6:24951035-24951054 MsG0680031704.01.T01:intron 25.0%
! AATTACCAACAAAAATATGC+AGG + Chr6:24952264-24952283 None:intergenic 25.0%
! ACTTCTATATTTGTATATGG+TGG + Chr6:24950438-24950457 None:intergenic 25.0%
! AGAAATGATCGGTTATAAAT+TGG - Chr6:24951320-24951339 MsG0680031704.01.T01:intron 25.0%
! AGCACTTCTATATTTGTATA+TGG + Chr6:24950441-24950460 None:intergenic 25.0%
! AGGAAATCATACATTTCATA+TGG - Chr6:24951371-24951390 MsG0680031704.01.T01:intron 25.0%
! ATAGGAGTGAAATTTATTAG+TGG + Chr6:24950319-24950338 None:intergenic 25.0%
! ATTCTATGTTTGATTGATTG+GGG + Chr6:24950121-24950140 None:intergenic 25.0%
! CTATTCTATGTTTGATTGAT+TGG + Chr6:24950123-24950142 None:intergenic 25.0%
! CTTTACACTATTATTGATAC+AGG - Chr6:24952395-24952414 MsG0680031704.01.T01:five_prime_UTR 25.0%
! GAAAATTAATGGGGAAAAAT+TGG - Chr6:24950524-24950543 MsG0680031704.01.T01:intron 25.0%
! GAATGCATAAATATGTGTTT+AGG + Chr6:24952372-24952391 None:intergenic 25.0%
! GTACAAATGTTGAAAGATAA+TGG - Chr6:24950661-24950680 MsG0680031704.01.T01:intron 25.0%
! TAAAAAAAAAAACGGTGTGT+TGG - Chr6:24952222-24952241 MsG0680031704.01.T01:five_prime_UTR 25.0%
! TATTATTTGTGTGTGAATCA+GGG - Chr6:24952284-24952303 MsG0680031704.01.T01:five_prime_UTR 25.0%
! TCATAAACATCAGCATTTAA+GGG + Chr6:24952556-24952575 None:intergenic 25.0%
! TCGTAAAAAATCAAACCAAA+CGG + Chr6:24952105-24952124 None:intergenic 25.0%
! TCTTTGAGTGAAAATTAATG+GGG - Chr6:24950515-24950534 MsG0680031704.01.T01:intron 25.0%
! TTAATGTCAATTATTGGACT+AGG - Chr6:24951244-24951263 MsG0680031704.01.T01:intron 25.0%
! TTATGTTTGAGTAGTTACTT+TGG - Chr6:24951587-24951606 MsG0680031704.01.T01:intron 25.0%
! TTATTATTTGTGTGTGAATC+AGG - Chr6:24952283-24952302 MsG0680031704.01.T01:five_prime_UTR 25.0%
! TTCATAAACATCAGCATTTA+AGG + Chr6:24952557-24952576 None:intergenic 25.0%
! TTTCCTTACACACTAAAAAT+AGG + Chr6:24950337-24950356 None:intergenic 25.0%
!! AAATAACAAATGAAGAGTAC+AGG + Chr6:24952185-24952204 None:intergenic 25.0%
!! AATAACAAATGAAGAGTACA+GGG + Chr6:24952184-24952203 None:intergenic 25.0%
!!! ATGATGATGAGTACAATTTT+TGG - Chr6:24950571-24950590 MsG0680031704.01.T01:intron 25.0%
!!! GAAAAATTGGTTTTCAAGAT+GGG - Chr6:24950537-24950556 MsG0680031704.01.T01:intron 25.0%
!!! TGCATTTTTTTTTTTATGGC+TGG - Chr6:24951615-24951634 MsG0680031704.01.T01:intron 25.0%
AACAAAAACTCACACATCAT+GGG + Chr6:24952063-24952082 None:intergenic 30.0%
AACATTTGTACAACTGTATC+TGG + Chr6:24950652-24950671 None:intergenic 30.0%
ACCTAATCCAACTTGATTTA+GGG + Chr6:24952512-24952531 None:intergenic 30.0%
ACTCGTTATAACTTCAAATC+TGG - Chr6:24950834-24950853 MsG0680031704.01.T01:intron 30.0%
ATGAGTTATTCGATCTTGAT+CGG + Chr6:24951415-24951434 None:intergenic 30.0%
ATGTATGTGGTGTGAAAATA+AGG + Chr6:24950003-24950022 None:intergenic 30.0%
CAAAACTAGATCTTAATTGG+TGG - Chr6:24951816-24951835 MsG0680031704.01.T01:CDS 30.0%
TAACAAAAACTCACACATCA+TGG + Chr6:24952064-24952083 None:intergenic 30.0%
TGTATGTGGTGTGAAAATAA+GGG + Chr6:24950002-24950021 None:intergenic 30.0%
TTGAAGTTCACACATATAAG+TGG + Chr6:24951115-24951134 None:intergenic 30.0%
! GGCATTTTGAATGTTCATTA+GGG + Chr6:24952491-24952510 None:intergenic 30.0%
! TCATTTTCTTTCTTCACTTG+GGG - Chr6:24950159-24950178 MsG0680031704.01.T01:intron 30.0%
! TTTATATTTCACTTGCTGGT+TGG - Chr6:24950952-24950971 MsG0680031704.01.T01:intron 30.0%
!! AGTCATTTTCTTTCTTCACT+TGG - Chr6:24950157-24950176 MsG0680031704.01.T01:intron 30.0%
!! GTCATTTTCTTTCTTCACTT+GGG - Chr6:24950158-24950177 MsG0680031704.01.T01:intron 30.0%
!!! ACTCCTATTTTTAGTGTGTA+AGG - Chr6:24950331-24950350 MsG0680031704.01.T01:intron 30.0%
!!! GGAAAAATTGGTTTTCAAGA+TGG - Chr6:24950536-24950555 MsG0680031704.01.T01:intron 30.0%
!!! TACAATTTTTGGTATGTGGA+AGG - Chr6:24950582-24950601 MsG0680031704.01.T01:intron 30.0%
!!! TGAGTACAATTTTTGGTATG+TGG - Chr6:24950578-24950597 MsG0680031704.01.T01:intron 30.0%
!!! TGGTTTTATATTTCACTTGC+TGG - Chr6:24950948-24950967 MsG0680031704.01.T01:intron 30.0%
AATTGTTGTGGATGTGATCA+TGG - Chr6:24950928-24950947 MsG0680031704.01.T01:intron 35.0%
ACATATAAGTGGAAGGATCA+GGG + Chr6:24951104-24951123 None:intergenic 35.0%
AGTAAGAAGAAGCTAGGAAA+TGG + Chr6:24950252-24950271 None:intergenic 35.0%
AGTTCACACATATAAGTGGA+AGG + Chr6:24951111-24951130 None:intergenic 35.0%
ATTATGATCGTGCTTTGCAA+TGG - Chr6:24950796-24950815 MsG0680031704.01.T01:intron 35.0%
ATTGAACTCAGAGAGTGATA+TGG - Chr6:24951544-24951563 MsG0680031704.01.T01:intron 35.0%
CAAAATGCCCTAAATCAAGT+TGG - Chr6:24952502-24952521 MsG0680031704.01.T01:five_prime_UTR 35.0%
CACCTAATCCAACTTGATTT+AGG + Chr6:24952513-24952532 None:intergenic 35.0%
CGTTATAACTTCAAATCTGG+TGG - Chr6:24950837-24950856 MsG0680031704.01.T01:intron 35.0%
GCTAAAAAGCTAGAACAAGT+TGG + Chr6:24950079-24950098 None:intergenic 35.0%
GTTCATCAGCTGGAATATTT+AGG - Chr6:24952598-24952617 MsG0680031704.01.T01:five_prime_UTR 35.0%
TGAACAAGTAAGAAGAAGCT+AGG + Chr6:24950258-24950277 None:intergenic 35.0%
TGAACAGCATTGTTTGAATC+TGG + Chr6:24952583-24952602 None:intergenic 35.0%
TTATGATCGTGCTTTGCAAT+GGG - Chr6:24950797-24950816 MsG0680031704.01.T01:intron 35.0%
TTTGAATGTTCATTAGGGCA+GGG + Chr6:24952486-24952505 None:intergenic 35.0%
! ACAACTGTATCTGGTTTCAA+TGG + Chr6:24950643-24950662 None:intergenic 35.0%
! CAAATGCTTTTGTTCTGACA+TGG - Chr6:24951645-24951664 MsG0680031704.01.T01:intron 35.0%
! CAGAGAGTGATATGGAATTT+TGG - Chr6:24951552-24951571 MsG0680031704.01.T01:intron 35.0%
! GGGCATTTTGAATGTTCATT+AGG + Chr6:24952492-24952511 None:intergenic 35.0%
! TGGATTAGGTGACTCAATTA+AGG - Chr6:24952522-24952541 MsG0680031704.01.T01:five_prime_UTR 35.0%
! TTTACGACGTATTCCCATTT+TGG - Chr6:24952118-24952137 MsG0680031704.01.T01:five_prime_UTR 35.0%
! TTTTGAATGTTCATTAGGGC+AGG + Chr6:24952487-24952506 None:intergenic 35.0%
!! AGAACAAAAGCATTTGCCAT+TGG + Chr6:24951641-24951660 None:intergenic 35.0%
!!! TTTTTTTTATGGCTGGCCAA+TGG - Chr6:24951622-24951641 MsG0680031704.01.T01:intron 35.0%
AAACAATGCTGTTCATCAGC+TGG - Chr6:24952588-24952607 MsG0680031704.01.T01:five_prime_UTR 40.0%
AAACTATGAGTGCACTTGGT+GGG - Chr6:24950718-24950737 MsG0680031704.01.T01:intron 40.0%
AAGAAGCTAGGAAATGGAGT+GGG + Chr6:24950246-24950265 None:intergenic 40.0%
ACAAAAATATGCAGGAGCTG+AGG + Chr6:24952256-24952275 None:intergenic 40.0%
ACCCAGGTACAATCCAAAAT+GGG + Chr6:24952134-24952153 None:intergenic 40.0%
ATGTGGTGTGAAAATAAGGG+AGG + Chr6:24949999-24950018 None:intergenic 40.0%
CACATATAAGTGGAAGGATC+AGG + Chr6:24951105-24951124 None:intergenic 40.0%
GAAGAAACTATGAGTGCACT+TGG - Chr6:24950714-24950733 MsG0680031704.01.T01:intron 40.0%
GTTATTCGATCTTGATCGGA+CGG + Chr6:24951411-24951430 None:intergenic 40.0%
TGATACAGGTATGTAGCAGT+TGG - Chr6:24952409-24952428 MsG0680031704.01.T01:five_prime_UTR 40.0%
! ATTTTGGATTGTACCTGGGT+CGG - Chr6:24952134-24952153 MsG0680031704.01.T01:five_prime_UTR 40.0%
! TCCCATTTTGGATTGTACCT+GGG - Chr6:24952130-24952149 MsG0680031704.01.T01:five_prime_UTR 40.0%
! TGCTTTTGTTCTGACATGGA+CGG - Chr6:24951649-24951668 MsG0680031704.01.T01:intron 40.0%
! TTCCCATTTTGGATTGTACC+TGG - Chr6:24952129-24952148 MsG0680031704.01.T01:five_prime_UTR 40.0%
! TTTTGGTATGTGGAAGGACT+TGG - Chr6:24950588-24950607 MsG0680031704.01.T01:intron 40.0%
!! ATCAGCATTTAAGGGTACTG+TGG + Chr6:24952548-24952567 None:intergenic 40.0%
!! CAGCTCCTGCATATTTTTGT+TGG - Chr6:24952256-24952275 MsG0680031704.01.T01:five_prime_UTR 40.0%
!! GCCCTAAATCAAGTTGGATT+AGG - Chr6:24952508-24952527 MsG0680031704.01.T01:five_prime_UTR 40.0%
!! TGGGTCAGGTATTGAAGTAA+TGG - Chr6:24950737-24950756 MsG0680031704.01.T01:intron 40.0%
AATGTTCATTAGGGCAGGGA+AGG + Chr6:24952482-24952501 None:intergenic 45.0%
AGAGTACAGGGAGAGAGATT+GGG + Chr6:24952172-24952191 None:intergenic 45.0%
GAAACTATGAGTGCACTTGG+TGG - Chr6:24950717-24950736 MsG0680031704.01.T01:intron 45.0%
GAAGAAGCTAGGAAATGGAG+TGG + Chr6:24950247-24950266 None:intergenic 45.0%
GACCCAGGTACAATCCAAAA+TGG + Chr6:24952135-24952154 None:intergenic 45.0%
! AAGAGTACAGGGAGAGAGAT+TGG + Chr6:24952173-24952192 None:intergenic 45.0%
! ATCGGACGGTTGATATCATG+TGG + Chr6:24951397-24951416 None:intergenic 45.0%
! TAGTTTAGGGCTACACCGTT+TGG - Chr6:24952087-24952106 MsG0680031704.01.T01:five_prime_UTR 45.0%
! TTTTGGATTGTACCTGGGTC+GGG - Chr6:24952135-24952154 MsG0680031704.01.T01:five_prime_UTR 45.0%
!! GAAGGACTTGGTGTGAATTG+GGG - Chr6:24950600-24950619 MsG0680031704.01.T01:intron 45.0%
!! GGAAGGACTTGGTGTGAATT+GGG - Chr6:24950599-24950618 MsG0680031704.01.T01:intron 45.0%
!! TGGAAGGACTTGGTGTGAAT+TGG - Chr6:24950598-24950617 MsG0680031704.01.T01:intron 45.0%
!! AAAGAAATTTAAAAATAATT+TGG + Chr6:24950470-24950489 None:intergenic 5.0%
AACCCAACAAGCCTGCATAC+AGG + Chr6:24952040-24952059 None:intergenic 50.0%
ACTTCCGACCTTCTCGATCA+CGG + Chr6:24951064-24951083 None:intergenic 50.0%
AGAAGGTCGGAAGTCGTGAT+CGG - Chr6:24951070-24951089 MsG0680031704.01.T01:intron 50.0%
AGTACAGGGAGAGAGATTGG+GGG + Chr6:24952170-24952189 None:intergenic 50.0%
GAGTACAGGGAGAGAGATTG+GGG + Chr6:24952171-24952190 None:intergenic 50.0%
! TGCCTGTATGCAGGCTTGTT+GGG - Chr6:24952035-24952054 MsG0680031704.01.T01:five_prime_UTR 50.0%
CTGGTTTCGTGCCTGTATGC+AGG - Chr6:24952026-24952045 MsG0680031704.01.T01:five_prime_UTR 55.0%
GAAGCCGTGATCGAGAAGGT+CGG - Chr6:24951057-24951076 MsG0680031704.01.T01:intron 55.0%
GAAGGTCGGAAGTCGTGATC+GGG - Chr6:24951071-24951090 MsG0680031704.01.T01:intron 55.0%
GTACAGGGAGAGAGATTGGG+GGG + Chr6:24952169-24952188 None:intergenic 55.0%
GTGCCTGTATGCAGGCTTGT+TGG - Chr6:24952034-24952053 MsG0680031704.01.T01:five_prime_UTR 55.0%
! ATGAGTGCACTTGGTGGGTC+AGG - Chr6:24950723-24950742 MsG0680031704.01.T01:intron 55.0%
GTCGGAAGCCGTGATCGAGA+AGG - Chr6:24951053-24951072 MsG0680031704.01.T01:intron 60.0%
GGGGACATTAGACCCGACCC+AGG + Chr6:24952150-24952169 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 24949956 24952633 24949956 ID=MsG0680031704.01;Name=MsG0680031704.01
Chr6 mRNA 24949956 24952633 24949956 ID=MsG0680031704.01.T01;Parent=MsG0680031704.01;Name=MsG0680031704.01.T01;_AED=0.21;_eAED=0.21;_QI=767|1|1|1|1|1|2|0|121
Chr6 exon 24949956 24950172 24949956 ID=MsG0680031704.01.T01:exon:15390;Parent=MsG0680031704.01.T01
Chr6 exon 24951718 24952633 24951718 ID=MsG0680031704.01.T01:exon:15389;Parent=MsG0680031704.01.T01
Chr6 five_prime_UTR 24951867 24952633 24951867 ID=MsG0680031704.01.T01:five_prime_utr;Parent=MsG0680031704.01.T01
Chr6 CDS 24951718 24951866 24951718 ID=MsG0680031704.01.T01:cds;Parent=MsG0680031704.01.T01
Chr6 CDS 24949956 24950172 24949956 ID=MsG0680031704.01.T01:cds;Parent=MsG0680031704.01.T01
Gene Sequence

>MsG0680031704.01.T01

ATGAGTGCACTTGGTGGGTCAGGTATTGAAGTAATGGTTGCTATTCCTAATAATCAATTAGCTGAAATGAGTTATTATGATCGTGCTTTGCAATGGGTTAGAAAGAATGTTACTCGTTATAACTTCAAATCTGGTGGAGTAAATATCAAGTATGTAGCAGTTGGAAATGAGCCATTTTTGAAATCATACAACAATTCATTCTTGAATGTAACCTTCCCTGCCCTAATGAACATTCAAAATGCCCTAAATCAAGTTGGATTAGGTGACTCAATTAAGGCCACAGTACCCTTAAATGCTGATGTTTATGAATCTCCAGATTCAAACAATGCTGTTCATCAGCTGGAATATTTAGGCCTGATTAAGTGA
Protein sequence

>MsG0680031704.01.T01

MSALGGSGIEVMVAIPNNQLAEMSYYDRALQWVRKNVTRYNFKSGGVNIKYVAVGNEPFLKSYNNSFLNVTFPALMNIQNALNQVGLGDSIKATVPLNADVYESPDSNNAVHQLEYLGLIK*