AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044318.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044318.01.T01 MTR_8g461030 75.214 117 4 2 13 128 15 107 5.27e-41 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATCAAATTTGTGAATT+AGG 0.114884 8:+39916389 None:intergenic
TTCTGTGGTGTTTGGGTTGT+TGG 0.214260 8:+39916276 None:intergenic
GGAATCAAATTTGTGAATTA+GGG 0.280894 8:+39916390 None:intergenic
GATTGAATTTCTGTGGTGTT+TGG 0.303191 8:+39916268 None:intergenic
CAAAACTTTCTTCTTCAATT+CGG 0.305134 8:-39916327 MsG0880044318.01.T01:CDS
ATTGAATTTCTGTGGTGTTT+GGG 0.351834 8:+39916269 None:intergenic
GGATGGTGTCTGTGCTTTGC+AGG 0.354391 8:-39914810 MsG0880044318.01.T01:CDS
AAAACTTTCTTCTTCAATTC+GGG 0.362346 8:-39916326 MsG0880044318.01.T01:CDS
AAATTTGTGAATTAGGGTAT+TGG 0.378091 8:+39916396 None:intergenic
AATTTGTGAATTAGGGTATT+GGG 0.379174 8:+39916397 None:intergenic
TGAAGGCCACAACAAAAGTT+TGG 0.389427 8:+39916369 None:intergenic
TTCTTCTTCAATTCGGGCAA+TGG 0.398305 8:-39916320 MsG0880044318.01.T01:CDS
TCTTCTTCAATTCGGGCAAT+GGG 0.417674 8:-39916319 MsG0880044318.01.T01:CDS
GAATTTGTGCAGCAGCTGAT+AGG 0.447579 8:+39916422 None:intergenic
AATCTTTAAGTGATGAAGAA+TGG 0.463954 8:-39914767 MsG0880044318.01.T01:CDS
GGAACTGAGAGAGCTTTCAC+TGG 0.500884 8:-39914699 MsG0880044318.01.T01:CDS
AACACCACAGAAATTCAATC+AGG 0.516707 8:-39916265 MsG0880044318.01.T01:CDS
ATAACCTGATTGAATTTCTG+TGG 0.652531 8:+39916261 None:intergenic
GAACTGAGAGAGCTTTCACT+GGG 0.658532 8:-39914698 MsG0880044318.01.T01:CDS
GGTTTAGTATGCAAACGTGA+AGG 0.665080 8:+39916352 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAGTATTAAGTTATGT+TGG + Chr8:39915017-39915036 None:intergenic 15.0%
!! TCAGAAGAAAAAAAAATATA+CGG - Chr8:39916175-39916194 MsG0880044318.01.T01:intron 15.0%
!!! TTTTTTTTTTTCTTCTGATA+GGG - Chr8:39916306-39916325 MsG0880044318.01.T01:CDS 15.0%
!!! TTTTTTTTTTTTCTTCTGAT+AGG - Chr8:39916305-39916324 MsG0880044318.01.T01:CDS 15.0%
!! AAAATGAAAATAGGAGAAAT+TGG - Chr8:39915490-39915509 MsG0880044318.01.T01:intron 20.0%
!! AAATTGATTATTCAATTTGC+TGG - Chr8:39915369-39915388 MsG0880044318.01.T01:intron 20.0%
!! TCTATATTGATAAGTTTAGA+AGG - Chr8:39915060-39915079 MsG0880044318.01.T01:intron 20.0%
!!! ATATTTTGATAGAAACAAAC+TGG - Chr8:39915035-39915054 MsG0880044318.01.T01:intron 20.0%
!!! ATGAACAATTTTATGTTACT+CGG - Chr8:39916409-39916428 MsG0880044318.01.T01:CDS 20.0%
! AAAACTTTCTTCTTCAATTC+GGG - Chr8:39914811-39914830 MsG0880044318.01.T01:CDS 25.0%
! AAATACATTTAACGTGTAGA+GGG - Chr8:39916011-39916030 MsG0880044318.01.T01:intron 25.0%
! AAATTTGTGAATTAGGGTAT+TGG + Chr8:39914744-39914763 None:intergenic 25.0%
! AATCTTTAAGTGATGAAGAA+TGG - Chr8:39916370-39916389 MsG0880044318.01.T01:CDS 25.0%
! ACACCCGAAAAAATATTATT+AGG - Chr8:39915189-39915208 MsG0880044318.01.T01:intron 25.0%
! CAAAACTTTCTTCTTCAATT+CGG - Chr8:39914810-39914829 MsG0880044318.01.T01:CDS 25.0%
! CACAAAATTGTACAAGAAAA+TGG - Chr8:39915419-39915438 MsG0880044318.01.T01:intron 25.0%
! CCGAAAAAATATTATTAGGA+GGG - Chr8:39915193-39915212 MsG0880044318.01.T01:intron 25.0%
! CGAAAAAATATTATTAGGAG+GGG - Chr8:39915194-39915213 MsG0880044318.01.T01:intron 25.0%
! GAAAAAATATTATTAGGAGG+GGG - Chr8:39915195-39915214 MsG0880044318.01.T01:intron 25.0%
! GGAATCAAATTTGTGAATTA+GGG + Chr8:39914750-39914769 None:intergenic 25.0%
! TCTACACGTTAAATGTATTT+CGG + Chr8:39916011-39916030 None:intergenic 25.0%
! TGGAATCAAATTTGTGAATT+AGG + Chr8:39914751-39914770 None:intergenic 25.0%
! TTTAGAAGGAGAAAAAAATG+CGG - Chr8:39915074-39915093 MsG0880044318.01.T01:intron 25.0%
!! AATTGAAGAAGAAAGTTTTG+TGG + Chr8:39914810-39914829 None:intergenic 25.0%
!! AATTTGTGAATTAGGGTATT+GGG + Chr8:39914743-39914762 None:intergenic 25.0%
!! ATTTTATGTTACTCGGAAAA+AGG - Chr8:39916416-39916435 MsG0880044318.01.T01:CDS 25.0%
!! TTGTATGTTGGTTATAAAGT+TGG - Chr8:39915856-39915875 MsG0880044318.01.T01:intron 25.0%
!! TTTTATGTTACTCGGAAAAA+GGG - Chr8:39916417-39916436 MsG0880044318.01.T01:CDS 25.0%
!!! ATTGAAGAAGAAAGTTTTGT+GGG + Chr8:39914809-39914828 None:intergenic 25.0%
!!! CCCTCCTAATAATATTTTTT+CGG + Chr8:39915196-39915215 None:intergenic 25.0%
!!! CCTCCTAATAATATTTTTTC+GGG + Chr8:39915195-39915214 None:intergenic 25.0%
AATACATTTAACGTGTAGAG+GGG - Chr8:39916012-39916031 MsG0880044318.01.T01:intron 30.0%
AATTGTACAAGAAAATGGTG+AGG - Chr8:39915424-39915443 MsG0880044318.01.T01:intron 30.0%
ATAACCTGATTGAATTTCTG+TGG + Chr8:39914879-39914898 None:intergenic 30.0%
ATTGAATTTCTGTGGTGTTT+GGG + Chr8:39914871-39914890 None:intergenic 30.0%
ATTGTACAAGAAAATGGTGA+GGG - Chr8:39915425-39915444 MsG0880044318.01.T01:intron 30.0%
CCCGAAAAAATATTATTAGG+AGG - Chr8:39915192-39915211 MsG0880044318.01.T01:intron 30.0%
GAAATACATTTAACGTGTAG+AGG - Chr8:39916010-39916029 MsG0880044318.01.T01:intron 30.0%
GCTGAAGTGAAAATGAAAAT+AGG - Chr8:39915481-39915500 MsG0880044318.01.T01:intron 30.0%
GTCAGACTATACTTACTTAA+CGG - Chr8:39915112-39915131 MsG0880044318.01.T01:intron 30.0%
TTAGAAGGAGAAAAAAATGC+GGG - Chr8:39915075-39915094 MsG0880044318.01.T01:intron 30.0%
! TATGTTGGTTATAAAGTTGG+TGG - Chr8:39915859-39915878 MsG0880044318.01.T01:intron 30.0%
! TTGATTCCAAACTTTTGTTG+TGG - Chr8:39914762-39914781 MsG0880044318.01.T01:CDS 30.0%
!! TAATGCTTGCTGTTTAAAGT+TGG - Chr8:39915963-39915982 MsG0880044318.01.T01:intron 30.0%
!!! TATTCAATTTGCTGGCTTTT+TGG - Chr8:39915377-39915396 MsG0880044318.01.T01:intron 30.0%
!!! TTTTTTTCTTCTGATAGGGA+TGG - Chr8:39916310-39916329 MsG0880044318.01.T01:CDS 30.0%
AACACCACAGAAATTCAATC+AGG - Chr8:39914872-39914891 MsG0880044318.01.T01:intron 35.0%
ACTCTTCAGACACAACATTA+AGG + Chr8:39915531-39915550 None:intergenic 35.0%
ATTACCAGAGTCATTTCCTT+GGG - Chr8:39915146-39915165 MsG0880044318.01.T01:intron 35.0%
CCATTAATAACTCTGCTTGT+AGG - Chr8:39915218-39915237 MsG0880044318.01.T01:intron 35.0%
CCTACAAGCAGAGTTATTAA+TGG + Chr8:39915221-39915240 None:intergenic 35.0%
CTCTTCAGACACAACATTAA+GGG + Chr8:39915530-39915549 None:intergenic 35.0%
GATTGAATTTCTGTGGTGTT+TGG + Chr8:39914872-39914891 None:intergenic 35.0%
TAACCAACATACAACTCTTC+AGG + Chr8:39915850-39915869 None:intergenic 35.0%
! TTGAATGCCCATTGTTTTCA+AGG - Chr8:39915287-39915306 MsG0880044318.01.T01:intron 35.0%
!! CTTGCTGTTTAAAGTTGGTT+GGG - Chr8:39915968-39915987 MsG0880044318.01.T01:intron 35.0%
!!! CTCTATGCTATTTTCAGTGA+TGG - Chr8:39916060-39916079 MsG0880044318.01.T01:intron 35.0%
AACTCTGCTTGTAGGTGTAT+GGG - Chr8:39915226-39915245 MsG0880044318.01.T01:intron 40.0%
ATAGAGATAAACACCTGCAG+TGG - Chr8:39916263-39916282 MsG0880044318.01.T01:CDS 40.0%
ATTGAGGTTAAGTCATCACC+TGG - Chr8:39915759-39915778 MsG0880044318.01.T01:intron 40.0%
CACACTTCTGCAACAGATTT+GGG + Chr8:39915648-39915667 None:intergenic 40.0%
CGCACAACCTTGAAAACAAT+GGG + Chr8:39915297-39915316 None:intergenic 40.0%
GATTACCAGAGTCATTTCCT+TGG - Chr8:39915145-39915164 MsG0880044318.01.T01:intron 40.0%
GGTTTAGTATGCAAACGTGA+AGG + Chr8:39914788-39914807 None:intergenic 40.0%
TAACTCTGCTTGTAGGTGTA+TGG - Chr8:39915225-39915244 MsG0880044318.01.T01:intron 40.0%
TCGCACAACCTTGAAAACAA+TGG + Chr8:39915298-39915317 None:intergenic 40.0%
TCTTCTTCAATTCGGGCAAT+GGG - Chr8:39914818-39914837 MsG0880044318.01.T01:intron 40.0%
TGAAGGCCACAACAAAAGTT+TGG + Chr8:39914771-39914790 None:intergenic 40.0%
TGAAGGCTCAATGTTGAGAT+TGG - Chr8:39915806-39915825 MsG0880044318.01.T01:intron 40.0%
TTCTTCTTCAATTCGGGCAA+TGG - Chr8:39914817-39914836 MsG0880044318.01.T01:intron 40.0%
TTTCAGTGATGGTAGTAGCA+AGG - Chr8:39916071-39916090 MsG0880044318.01.T01:intron 40.0%
! CAAATCTGTTGCAGAAGTGT+GGG - Chr8:39915647-39915666 MsG0880044318.01.T01:intron 40.0%
! TGTTAATCCTCTAGTTCCCA+AGG + Chr8:39915165-39915184 None:intergenic 40.0%
! TTGGAGTGGCAAATTGCTTT+TGG - Chr8:39915717-39915736 MsG0880044318.01.T01:intron 40.0%
! TTTTGCCGTGTAGTGTGTAT+TGG - Chr8:39915564-39915583 MsG0880044318.01.T01:intron 40.0%
!! ATTGTTTTCAAGGTTGTGCG+AGG - Chr8:39915297-39915316 MsG0880044318.01.T01:intron 40.0%
!! GCTTGCTGTTTAAAGTTGGT+TGG - Chr8:39915967-39915986 MsG0880044318.01.T01:intron 40.0%
!!! AATTTGCTGGCTTTTTGGAG+TGG - Chr8:39915382-39915401 MsG0880044318.01.T01:intron 40.0%
AACTATGGCTTCGCATGAGA+TGG - Chr8:39915681-39915700 MsG0880044318.01.T01:intron 45.0%
ACAACTCTTCAGGTCCATGA+TGG + Chr8:39915840-39915859 None:intergenic 45.0%
ACATTTAACGTGTAGAGGGG+TGG - Chr8:39916015-39916034 MsG0880044318.01.T01:intron 45.0%
ACCAAGCACATGAGATGCAA+AGG + Chr8:39916116-39916135 None:intergenic 45.0%
ACTCTGCTTGTAGGTGTATG+GGG - Chr8:39915227-39915246 MsG0880044318.01.T01:intron 45.0%
AGGCTCAATGTTGAGATTGG+TGG - Chr8:39915809-39915828 MsG0880044318.01.T01:intron 45.0%
AGTTCCCAAGGAAATGACTC+TGG + Chr8:39915153-39915172 None:intergenic 45.0%
CCAAATCTGTTGCAGAAGTG+TGG - Chr8:39915646-39915665 MsG0880044318.01.T01:intron 45.0%
CCACACTTCTGCAACAGATT+TGG + Chr8:39915649-39915668 None:intergenic 45.0%
CTCATGCGAAGCCATAGTTT+GGG + Chr8:39915680-39915699 None:intergenic 45.0%
CTTACTTAACGGTCGAACTC+AGG - Chr8:39915123-39915142 MsG0880044318.01.T01:intron 45.0%
GAACTGAGAGAGCTTTCACT+GGG - Chr8:39916439-39916458 MsG0880044318.01.T01:CDS 45.0%
GAATTTGTGCAGCAGCTGAT+AGG + Chr8:39914718-39914737 None:intergenic 45.0%
GAGTGGAATTAATAGGCGTG+GGG - Chr8:39915933-39915952 MsG0880044318.01.T01:intron 45.0%
GGACCTGAAGAGTTGTATGT+TGG - Chr8:39915844-39915863 MsG0880044318.01.T01:intron 45.0%
GGAGTGGAATTAATAGGCGT+GGG - Chr8:39915932-39915951 MsG0880044318.01.T01:intron 45.0%
GTCATTTCCTTGGGAACTAG+AGG - Chr8:39915155-39915174 MsG0880044318.01.T01:intron 45.0%
TCCTTTGCATCTCATGTGCT+TGG - Chr8:39916112-39916131 MsG0880044318.01.T01:intron 45.0%
TCTCATGCGAAGCCATAGTT+TGG + Chr8:39915681-39915700 None:intergenic 45.0%
TGGAGTGGAATTAATAGGCG+TGG - Chr8:39915931-39915950 MsG0880044318.01.T01:intron 45.0%
TGGGAAGCTATCCCAAACTA+TGG - Chr8:39915666-39915685 MsG0880044318.01.T01:intron 45.0%
! CGGACTGGAGTGGAATTAAT+AGG - Chr8:39915926-39915945 MsG0880044318.01.T01:intron 45.0%
! GATTGGTGGCTTAACCATCA+TGG - Chr8:39915823-39915842 MsG0880044318.01.T01:intron 45.0%
! TAAAAGCGTGTTCCCGTCTT+CGG - Chr8:39915906-39915925 MsG0880044318.01.T01:intron 45.0%
!! GAGTGGCAAATTGCTTTTGG+TGG - Chr8:39915720-39915739 MsG0880044318.01.T01:intron 45.0%
!! GGCAAATTGCTTTTGGTGGA+GGG - Chr8:39915724-39915743 MsG0880044318.01.T01:intron 45.0%
!! TGGATCTGTGCTGATTAGTG+CGG - Chr8:39915779-39915798 MsG0880044318.01.T01:intron 45.0%
!! TGGCAAATTGCTTTTGGTGG+AGG - Chr8:39915723-39915742 MsG0880044318.01.T01:intron 45.0%
!! TTCTGTGGTGTTTGGGTTGT+TGG + Chr8:39914864-39914883 None:intergenic 45.0%
!! TTGCTTTTGGTGGAGGGTTT+TGG - Chr8:39915730-39915749 MsG0880044318.01.T01:intron 45.0%
!!! AGGGTTTTGGAGGGTTATTG+AGG - Chr8:39915743-39915762 MsG0880044318.01.T01:intron 45.0%
!!! TTTTGGTGGAGGGTTTTGGA+GGG - Chr8:39915734-39915753 MsG0880044318.01.T01:intron 45.0%
AGATGGTGAAGGGGCTTACT+TGG - Chr8:39915698-39915717 MsG0880044318.01.T01:intron 50.0%
ATTCCACTCCAGTCCGAAGA+CGG + Chr8:39915922-39915941 None:intergenic 50.0%
CCCTGCCAATACACACTACA+CGG + Chr8:39915572-39915591 None:intergenic 50.0%
CTCTGCTTGTAGGTGTATGG+GGG - Chr8:39915228-39915247 MsG0880044318.01.T01:intron 50.0%
CTGATTAGTGCGGCTCTTGA+AGG - Chr8:39915789-39915808 MsG0880044318.01.T01:intron 50.0%
CTTCGCATGAGATGGTGAAG+GGG - Chr8:39915689-39915708 MsG0880044318.01.T01:intron 50.0%
GCACTAATCAGCACAGATCC+AGG + Chr8:39915780-39915799 None:intergenic 50.0%
GCTTCGCATGAGATGGTGAA+GGG - Chr8:39915688-39915707 MsG0880044318.01.T01:intron 50.0%
GGAACTGAGAGAGCTTTCAC+TGG - Chr8:39916438-39916457 MsG0880044318.01.T01:CDS 50.0%
TATGGGGGGTTGTGATGTTG+AGG - Chr8:39915243-39915262 MsG0880044318.01.T01:intron 50.0%
TCTGCTTGTAGGTGTATGGG+GGG - Chr8:39915229-39915248 MsG0880044318.01.T01:intron 50.0%
! ATCTGCTGCAAAGTACCTCC+AGG + Chr8:39915609-39915628 None:intergenic 50.0%
! CCGTGTAGTGTGTATTGGCA+GGG - Chr8:39915569-39915588 MsG0880044318.01.T01:intron 50.0%
! GTAGTGTGTATTGGCAGGGT+GGG - Chr8:39915573-39915592 MsG0880044318.01.T01:intron 50.0%
! TGTAGTGTGTATTGGCAGGG+TGG - Chr8:39915572-39915591 MsG0880044318.01.T01:intron 50.0%
!! CTTTTGGTGGAGGGTTTTGG+AGG - Chr8:39915733-39915752 MsG0880044318.01.T01:intron 50.0%
!! GTTTTCAAGGTTGTGCGAGG+CGG - Chr8:39915300-39915319 MsG0880044318.01.T01:intron 50.0%
!! TGGAGGTACTTTGCAGCAGA+TGG - Chr8:39915608-39915627 MsG0880044318.01.T01:intron 50.0%
!!! TTGCTGGCTTTTTGGAGTGG+AGG - Chr8:39915385-39915404 MsG0880044318.01.T01:intron 50.0%
GGATGGTGTCTGTGCTTTGC+AGG - Chr8:39916327-39916346 MsG0880044318.01.T01:CDS 55.0%
GGCTTCGCATGAGATGGTGA+AGG - Chr8:39915687-39915706 MsG0880044318.01.T01:intron 55.0%
GGGGGTTGTGATGTTGAGGA+AGG - Chr8:39915247-39915266 MsG0880044318.01.T01:intron 55.0%
GTGAAGGGGCTTACTTGGAG+TGG - Chr8:39915703-39915722 MsG0880044318.01.T01:intron 55.0%
TTCCACTCCAGTCCGAAGAC+GGG + Chr8:39915921-39915940 None:intergenic 55.0%
! GACACTGTATGTGCCACTGC+AGG + Chr8:39916279-39916298 None:intergenic 55.0%
! GCCGTGTAGTGTGTATTGGC+AGG - Chr8:39915568-39915587 MsG0880044318.01.T01:intron 55.0%
!! CGGTAGTGGGTTGCTTGCTT+GGG - Chr8:39915320-39915339 MsG0880044318.01.T01:intron 55.0%
AACGTGTAGAGGGGTGGTGC+TGG - Chr8:39916021-39916040 MsG0880044318.01.T01:intron 60.0%
AAGGTTGTGCGAGGCGGTAG+TGG - Chr8:39915306-39915325 MsG0880044318.01.T01:intron 60.0%
AGGTTGTGCGAGGCGGTAGT+GGG - Chr8:39915307-39915326 MsG0880044318.01.T01:intron 60.0%
! TTCCCGTCTTCGGACTGGAG+TGG - Chr8:39915916-39915935 MsG0880044318.01.T01:intron 60.0%
!! GCGGTAGTGGGTTGCTTGCT+TGG - Chr8:39915319-39915338 MsG0880044318.01.T01:intron 60.0%
AGGGTGGGCAGCAGGAAACC+TGG - Chr8:39915588-39915607 MsG0880044318.01.T01:intron 65.0%
GCGTGTTCCCGTCTTCGGAC+TGG - Chr8:39915911-39915930 MsG0880044318.01.T01:intron 65.0%
GTGGGCAGCAGGAAACCTGG+AGG - Chr8:39915591-39915610 MsG0880044318.01.T01:intron 65.0%
! GTATTGGCAGGGTGGGCAGC+AGG - Chr8:39915580-39915599 MsG0880044318.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 39914695 39916464 39914695 ID=MsG0880044318.01;Name=MsG0880044318.01
Chr8 mRNA 39914695 39916464 39914695 ID=MsG0880044318.01.T01;Parent=MsG0880044318.01;Name=MsG0880044318.01.T01;_AED=0.49;_eAED=0.57;_QI=0|0|0|1|0|0|2|0|128
Chr8 exon 39916216 39916464 39916216 ID=MsG0880044318.01.T01:exon:3207;Parent=MsG0880044318.01.T01
Chr8 exon 39914695 39914832 39914695 ID=MsG0880044318.01.T01:exon:3206;Parent=MsG0880044318.01.T01
Chr8 CDS 39916216 39916464 39916216 ID=MsG0880044318.01.T01:cds;Parent=MsG0880044318.01.T01
Chr8 CDS 39914695 39914832 39914695 ID=MsG0880044318.01.T01:cds;Parent=MsG0880044318.01.T01
Gene Sequence

>MsG0880044318.01.T01

ATGGCATCTCATAGTTTGAGCCTATCAGCTGCTGCACAAATTCTTCCCAATACCCTAATTCACAAATTTGATTCCAAACTTTTGTTGTGGCCTTCACGTTTGCATACTAAACCCACAAAACTTTCTTCTTCAATTCGGGCAATGGGTTCTTCAGCTTCTTCTAACTCCAACAACCCAAACACCACAGAAATTCAATCAGGTTATTCATTATTCTATTGTGCTTTTCTTTGCTCAAATCAATTTACTTTAGGATGGTGTCTGTGCTTTGCAGGATCTAGTTCTGTTGATTATAAATCTTTAAGTGATGAAGAATGGAAGAAGCAGCTAACAGATGAACAATTTTATGTTACTCGGAAAAAGGGAACTGAGAGAGCTTTCACTGGGTGA

Protein sequence

>MsG0880044318.01.T01

MASHSLSLSAAAQILPNTLIHKFDSKLLLWPSRLHTKPTKLSSSIRAMGSSASSNSNNPNTTEIQSGYSLFYCAFLCSNQFTLGWCLCFAGSSSVDYKSLSDEEWKKQLTDEQFYVTRKKGTERAFTG*