AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018317.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018317.01.T01 MTR_4g009870 85.455 220 8 1 54 249 101 320 1.36e-120 348
MsG0480018317.01.T01 MTR_4g009870 34.682 173 111 2 78 249 4 175 7.93e-13 67.8
MsG0480018317.01.T01 MTR_4g009870 66.837 196 41 1 54 249 101 272 1.49e-77 238
MsG0480018317.01.T01 MTR_4g116170 62.319 207 54 1 67 249 310 516 2.71e-71 228
MsG0480018317.01.T01 MTR_4g116170 34.171 199 103 2 54 227 369 564 2.10e-16 78.6
MsG0480018317.01.T01 MTR_4g116170 29.333 225 129 4 54 249 172 395 1.02e-11 64.7
MsG0480018317.01.T01 MTR_4g116170 28.324 173 121 3 53 222 125 297 5.93e-11 62.4
MsG0480018317.01.T01 MTR_8g099415 29.082 196 126 4 51 233 287 482 5.10e-11 62.8
MsG0480018317.01.T01 MTR_8g099415 29.082 196 126 4 51 233 287 482 5.15e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018317.01.T01 AT1G15740 65.000 220 53 1 54 249 306 525 1.98e-77 244
MsG0480018317.01.T01 AT1G15740 42.424 132 69 4 92 218 148 277 1.04e-14 73.6

Find 62 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCTGAAGTCTCTTAATT+TGG 0.152359 4:-3265879 MsG0480018317.01.T01:CDS
TTGTTAGTGACAGTGGTTTA+AGG 0.217027 4:-3265919 MsG0480018317.01.T01:CDS
CTCACTGACCTTGATTTGTT+TGG 0.245633 4:-3265129 MsG0480018317.01.T01:CDS
TTAAGCAGGATTGACAAAAT+TGG 0.248392 4:-3267179 MsG0480018317.01.T01:intron
CCAGTCCAGCATCAGTAATT+TGG 0.287315 4:+3265849 None:intergenic
TTTGCTGATGCAGGTTTGAC+TGG 0.365454 4:-3265153 MsG0480018317.01.T01:intron
GCAAGGGTTGGAGAAATAAA+AGG 0.383866 4:+3267903 None:intergenic
AACCTTGATTCATGTAAGAT+TGG 0.403335 4:-3267148 MsG0480018317.01.T01:CDS
CCAAATTACTGATGCTGGAC+TGG 0.409005 4:-3265849 MsG0480018317.01.T01:CDS
TTTGACAGTGCAGGGAGCTC+TGG 0.422330 4:+3267653 None:intergenic
TCTTGTAGGATTGTCTAGTC+TGG 0.424185 4:-3265966 MsG0480018317.01.T01:intron
TTTCTGACAGAGGGTGTGAT+AGG 0.424800 4:-3267607 MsG0480018317.01.T01:CDS
AGATTCAAGTTTGACAGTGC+AGG 0.445204 4:+3267644 None:intergenic
AAAACTGTGGAGTTAATTGC+TGG 0.446777 4:-3264404 MsG0480018317.01.T01:CDS
AGCTGTTGGAAATGGTAGTC+GGG 0.460263 4:-3267941 None:intergenic
AAAGTTGAAAGTATTAAACT+TGG 0.469983 4:-3267422 MsG0480018317.01.T01:CDS
AGGAGTGACAAGACAGTTTG+AGG 0.470432 4:+3267873 None:intergenic
ATTCAAGAATCTGAGGTCCT+TGG 0.471185 4:-3264528 MsG0480018317.01.T01:CDS
GCTTACCAAATTACTGATGC+TGG 0.472779 4:-3265854 MsG0480018317.01.T01:CDS
ATCACAGAACAGCAACCTTA+CGG 0.480947 4:-3264429 MsG0480018317.01.T01:CDS
TATTAAATCAAAACTCCAAT+GGG 0.487405 4:+3267691 None:intergenic
CAGTTTGAGGGAGGAGTGCA+AGG 0.490470 4:+3267886 None:intergenic
GCGCTAGGATCACAGATGTT+GGG 0.491382 4:-3265107 MsG0480018317.01.T01:CDS
ACCTTGATTTGTTTGGCGCT+AGG 0.494505 4:-3265122 MsG0480018317.01.T01:CDS
TGAGGGAGGAGTGCAAGGGT+TGG 0.494611 4:+3267891 None:intergenic
GGCGCTAGGATCACAGATGT+TGG 0.508377 4:-3265108 MsG0480018317.01.T01:CDS
TTCACTGTTGTTAGTGACAG+TGG 0.508517 4:-3265926 MsG0480018317.01.T01:CDS
CACCAATCTTACATGAATCA+AGG 0.510275 4:+3267146 None:intergenic
CACTCCTTGAGTCATTAAAG+TGG 0.514676 4:-3267857 MsG0480018317.01.T01:CDS
CAGCTGTTGGAAATGGTAGT+CGG 0.515854 4:-3267942 None:intergenic
TGATGAAGGATTGGTGAACT+TGG 0.515944 4:-3267125 MsG0480018317.01.T01:CDS
GATTCAAGTTTGACAGTGCA+GGG 0.517023 4:+3267645 None:intergenic
CATGGGAACACATACTTGCA+AGG 0.524260 4:+3265822 None:intergenic
TCCTAGCGCCAAACAAATCA+AGG 0.529517 4:+3265121 None:intergenic
TCATGTAAGATTGGTGATGA+AGG 0.532453 4:-3267139 MsG0480018317.01.T01:CDS
GGAGTGACAAGACAGTTTGA+GGG 0.533418 4:+3267874 None:intergenic
GAAGGATTGGTGAACTTGGC+AGG 0.535990 4:-3267121 MsG0480018317.01.T01:intron
CTCTCCACTTTAATGACTCA+AGG 0.549649 4:+3267853 None:intergenic
AAGTTGAAAGTATTAAACTT+GGG 0.557845 4:-3267421 MsG0480018317.01.T01:CDS
GCATGTTTAGCTCATTTGAA+AGG 0.558223 4:-3267379 MsG0480018317.01.T01:intron
ATGGGAACACATACTTGCAA+GGG 0.563020 4:+3265823 None:intergenic
TAAGATTGGTGATGAAGGAT+TGG 0.567788 4:-3267134 MsG0480018317.01.T01:CDS
CTTGAGTCATTAAAGTGGAG+AGG 0.569050 4:-3267852 MsG0480018317.01.T01:CDS
ATATTAAATCAAAACTCCAA+TGG 0.574171 4:+3267690 None:intergenic
AATGAGGATGTAATACCCAT+TGG 0.574891 4:-3267706 MsG0480018317.01.T01:intron
AGCGGTGGATTGACTGATGC+TGG 0.575698 4:-3264497 MsG0480018317.01.T01:CDS
AACTTGGGATTTAATGACAT+AGG 0.577016 4:-3267406 MsG0480018317.01.T01:CDS
AAACAGAATTCAAGAATCTG+AGG 0.586780 4:-3264535 MsG0480018317.01.T01:intron
GATGCAATATTTCTGACAGA+GGG 0.606799 4:-3267616 MsG0480018317.01.T01:CDS
AAACTGTGGAGTTAATTGCT+GGG 0.614484 4:-3264403 MsG0480018317.01.T01:CDS
AGTTTGAGGGAGGAGTGCAA+GGG 0.614742 4:+3267887 None:intergenic
TCCACCGCTGCATATTTCCA+AGG 0.622364 4:+3264511 None:intergenic
GGGTTGGAGAAATAAAAGGT+TGG 0.641408 4:+3267907 None:intergenic
TCCTTGGAAATATGCAGCGG+TGG 0.643300 4:-3264512 MsG0480018317.01.T01:CDS
ACTGTCACTAACAACAGTGA+AGG 0.649551 4:+3265928 None:intergenic
GCTGTTGGAAATGGTAGTCG+GGG 0.649578 4:-3267940 None:intergenic
AGATGCAATATTTCTGACAG+AGG 0.657280 4:-3267617 MsG0480018317.01.T01:CDS
GTGACAAGACAGTTTGAGGG+AGG 0.657748 4:+3267877 None:intergenic
CAACCTTACGGACAAAACTG+TGG 0.677276 4:-3264417 MsG0480018317.01.T01:CDS
AGGTCCTTGGAAATATGCAG+CGG 0.704864 4:-3264515 MsG0480018317.01.T01:CDS
AGTGGTTTAAGGAAATTGTG+TGG 0.713167 4:-3265908 MsG0480018317.01.T01:CDS
CAAACAAATCAAGGTCAGTG+AGG 0.728596 4:+3265130 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAAAAAAAAAAAAAA+AGG + Chr4:3266339-3266358 None:intergenic 0.0%
!! AAAAAAATATGAAAAGAATG+TGG - Chr4:3266938-3266957 MsG0480018317.01.T01:intron 15.0%
!! AAAATAGAAATTGATAGATT+AGG - Chr4:3265298-3265317 MsG0480018317.01.T01:intron 15.0%
!! AAATTCAATCTTAATAATTG+TGG - Chr4:3267668-3267687 MsG0480018317.01.T01:CDS 15.0%
!! AATATTTACAATTCAAAGAA+TGG - Chr4:3266532-3266551 MsG0480018317.01.T01:intron 15.0%
!! ACAATTATTAAGATTGAATT+TGG + Chr4:3267669-3267688 None:intergenic 15.0%
!! ACATTCAAAAAAAAAAGAAA+AGG + Chr4:3266604-3266623 None:intergenic 15.0%
!! GTATAATTACATAATTTACA+GGG - Chr4:3264790-3264809 MsG0480018317.01.T01:intron 15.0%
!! TTAAAAAAAAAAATGTAAGC+TGG - Chr4:3265707-3265726 MsG0480018317.01.T01:intron 15.0%
!! TTGAATTGTAAATATTTCAT+GGG + Chr4:3266528-3266547 None:intergenic 15.0%
!! TTTGAATTGTAAATATTTCA+TGG + Chr4:3266529-3266548 None:intergenic 15.0%
!!! AATCTAAAGAATGATTTTAT+TGG - Chr4:3265775-3265794 MsG0480018317.01.T01:intron 15.0%
!!! TTTTTTTTTTGAATGTTGAA+TGG - Chr4:3266608-3266627 MsG0480018317.01.T01:intron 15.0%
!! AAAGAAAGAAATTTATTGTC+TGG + Chr4:3265543-3265562 None:intergenic 20.0%
!! AAATTCAAATAATTTATCCC+AGG + Chr4:3267089-3267108 None:intergenic 20.0%
!! AAGAAAGAAATTTATTGTCT+GGG + Chr4:3265542-3265561 None:intergenic 20.0%
!! AGCAAACAAAAATAAGTAAA+AGG + Chr4:3267357-3267376 None:intergenic 20.0%
!! ATAAAAAAGCTGAAATATGT+TGG - Chr4:3267382-3267401 MsG0480018317.01.T01:CDS 20.0%
!! ATACATTATGTTTGGATTAA+TGG + Chr4:3265651-3265670 None:intergenic 20.0%
!! ATAGGAATTGTATTTGAAAA+TGG - Chr4:3265098-3265117 MsG0480018317.01.T01:CDS 20.0%
!! ATATTAAATCAAAACTCCAA+TGG + Chr4:3264643-3264662 None:intergenic 20.0%
!! GGTATAATTACATAATTTAC+AGG - Chr4:3264789-3264808 MsG0480018317.01.T01:intron 20.0%
!! TAGAAAGCAATATAGAAATA+CGG - Chr4:3265405-3265424 MsG0480018317.01.T01:intron 20.0%
!! TATAAAATTACCTGTGTAAT+AGG + Chr4:3266846-3266865 None:intergenic 20.0%
!! TATTAAATCAAAACTCCAAT+GGG + Chr4:3264642-3264661 None:intergenic 20.0%
!! TTAATCCAAACATAATGTAT+TGG - Chr4:3265651-3265670 MsG0480018317.01.T01:intron 20.0%
!!! AAAGTTGAAAGTATTAAACT+TGG - Chr4:3264908-3264927 MsG0480018317.01.T01:intron 20.0%
!!! AAGTTGAAAGTATTAAACTT+GGG - Chr4:3264909-3264928 MsG0480018317.01.T01:intron 20.0%
!!! AGGTATAAAATCATATTTTC+AGG - Chr4:3266802-3266821 MsG0480018317.01.T01:intron 20.0%
!!! TAAATATTTACTTCCTTTTC+CGG - Chr4:3266656-3266675 MsG0480018317.01.T01:intron 20.0%
!!! TACTTATGTATATTTTAAGC+AGG - Chr4:3265137-3265156 MsG0480018317.01.T01:CDS 20.0%
!!! TTTTATATTCCATTTCTTGT+AGG - Chr4:3266350-3266369 MsG0480018317.01.T01:intron 20.0%
! AAAATAAGTAAAAGGCCAAA+CGG + Chr4:3267349-3267368 None:intergenic 25.0%
! ACAAACAATTATGAGTTGTT+TGG - Chr4:3266871-3266890 MsG0480018317.01.T01:intron 25.0%
! ACAAACTACTGGTAAAATAA+CGG - Chr4:3265042-3265061 MsG0480018317.01.T01:intron 25.0%
! CATAAACTAATCTCTTGAAA+TGG - Chr4:3266194-3266213 MsG0480018317.01.T01:intron 25.0%
! CTTGTGATTATAAAGTAACA+AGG + Chr4:3266161-3266180 None:intergenic 25.0%
! GAAGAAATAGGAATAGAAAT+GGG + Chr4:3265617-3265636 None:intergenic 25.0%
! TGGCATATAATAACTTAATG+AGG - Chr4:3264608-3264627 MsG0480018317.01.T01:intron 25.0%
!! AAATATTTACTTCCTTTTCC+GGG - Chr4:3266657-3266676 MsG0480018317.01.T01:intron 25.0%
!! ACATATTGACTACATTTATG+TGG - Chr4:3267314-3267333 MsG0480018317.01.T01:intron 25.0%
!! ATGATTTTATACCTTGAAAC+AGG + Chr4:3266796-3266815 None:intergenic 25.0%
!! TATTTGAAAAGTATGCAACA+TGG - Chr4:3267252-3267271 MsG0480018317.01.T01:intron 25.0%
!!! AAATGTGGAGAAAAGTTTTT+TGG - Chr4:3265510-3265529 MsG0480018317.01.T01:intron 25.0%
!!! AATGTGGAGAAAAGTTTTTT+GGG - Chr4:3265511-3265530 MsG0480018317.01.T01:intron 25.0%
!!! ATGTTTGGATTAATGGATTT+GGG + Chr4:3265644-3265663 None:intergenic 25.0%
!!! ATTAATGGTTTTACATGAGA+CGG + Chr4:3267588-3267607 None:intergenic 25.0%
!!! ATTCTAAAGCTAATTCTTTC+AGG - Chr4:3264767-3264786 MsG0480018317.01.T01:intron 25.0%
!!! GCAGTTAATTTTAAAGAACT+TGG + Chr4:3267698-3267717 None:intergenic 25.0%
!!! TATGTTTGGATTAATGGATT+TGG + Chr4:3265645-3265664 None:intergenic 25.0%
!!! TTCTAAAGCTAATTCTTTCA+GGG - Chr4:3264768-3264787 MsG0480018317.01.T01:intron 25.0%
!!! TTCTGATCATTTTTCCTATT+GGG - Chr4:3266054-3266073 MsG0480018317.01.T01:intron 25.0%
!!! TTTCTGATCATTTTTCCTAT+TGG - Chr4:3266053-3266072 MsG0480018317.01.T01:intron 25.0%
AAACAGAATTCAAGAATCTG+AGG - Chr4:3267795-3267814 MsG0480018317.01.T01:intron 30.0%
AAATAGGAATAGAAATGGGT+GGG + Chr4:3265613-3265632 None:intergenic 30.0%
AACCTTGATTCATGTAAGAT+TGG - Chr4:3265182-3265201 MsG0480018317.01.T01:intron 30.0%
AACTAATCTCTTGAAATGGT+TGG - Chr4:3266198-3266217 MsG0480018317.01.T01:intron 30.0%
AACTTGGGATTTAATGACAT+AGG - Chr4:3264924-3264943 MsG0480018317.01.T01:intron 30.0%
AAGGAGAAAGAAGAATATGA+AGG - Chr4:3265451-3265470 MsG0480018317.01.T01:intron 30.0%
AAGTTAGGTGATTCATACTA+AGG - Chr4:3266278-3266297 MsG0480018317.01.T01:intron 30.0%
ACTTATTCTGTACAAACTAC+TGG - Chr4:3265031-3265050 MsG0480018317.01.T01:intron 30.0%
AGTAATGAAAAGAACTTGTC+AGG - Chr4:3266227-3266246 MsG0480018317.01.T01:intron 30.0%
AGTTATTGTAGGAATGTTGA+TGG - Chr4:3264852-3264871 MsG0480018317.01.T01:intron 30.0%
ATATCCAGAAGTTTCTCTTT+CGG + Chr4:3265964-3265983 None:intergenic 30.0%
ATATTCCTTGTGGAATGAAT+GGG - Chr4:3265982-3266001 MsG0480018317.01.T01:intron 30.0%
CACTACCAATACATTATGTT+TGG + Chr4:3265659-3265678 None:intergenic 30.0%
CATTCCTACAATAACTGTTA+CGG + Chr4:3264848-3264867 None:intergenic 30.0%
GGAAGAAATAGGAATAGAAA+TGG + Chr4:3265618-3265637 None:intergenic 30.0%
GGAATAAAGCTAAATTCAAC+AGG + Chr4:3266140-3266159 None:intergenic 30.0%
GTAACTTAATGATGTCTTTC+TGG - Chr4:3264588-3264607 MsG0480018317.01.T01:intron 30.0%
GTAATGAAAAGAACTTGTCA+GGG - Chr4:3266228-3266247 MsG0480018317.01.T01:intron 30.0%
TATATTCCTTGTGGAATGAA+TGG - Chr4:3265981-3266000 MsG0480018317.01.T01:intron 30.0%
TCTCCAATTCTCTTTAAATG+TGG - Chr4:3265495-3265514 MsG0480018317.01.T01:intron 30.0%
TCTCCACATTTAAAGAGAAT+TGG + Chr4:3265501-3265520 None:intergenic 30.0%
TGAAACAAAGCATACAAGAA+AGG + Chr4:3265260-3265279 None:intergenic 30.0%
TGATCATATGCAAACAATTG+TGG + Chr4:3265756-3265775 None:intergenic 30.0%
TTAAGCAGGATTGACAAAAT+TGG - Chr4:3265151-3265170 MsG0480018317.01.T01:intron 30.0%
TTATTCCTGTGATAAAGTGT+GGG + Chr4:3267042-3267061 None:intergenic 30.0%
TTTATTCCTGTGATAAAGTG+TGG + Chr4:3267043-3267062 None:intergenic 30.0%
TTTCAATGGAGAAAACGATA+GGG - Chr4:3265275-3265294 MsG0480018317.01.T01:intron 30.0%
TTTGCTAGAGTAGAAAAGAT+AGG - Chr4:3265080-3265099 MsG0480018317.01.T01:intron 30.0%
! ACAAGTTCTTTTCATTACTC+AGG + Chr4:3266226-3266245 None:intergenic 30.0%
! ATAATTTACAGGGTGCTTAT+TGG - Chr4:3264800-3264819 MsG0480018317.01.T01:intron 30.0%
! CTTTGATTTAACCCATATGT+CGG - Chr4:3267733-3267752 MsG0480018317.01.T01:intron 30.0%
! TAATTTACAGGGTGCTTATT+GGG - Chr4:3264801-3264820 MsG0480018317.01.T01:intron 30.0%
! TATTTCTTCACAACGCTTTT+AGG - Chr4:3265826-3265845 MsG0480018317.01.T01:intron 30.0%
! TGTGAAAGTACATCTCATAA+TGG + Chr4:3266022-3266041 None:intergenic 30.0%
! TTCTAGAAATCCTATTACAC+AGG - Chr4:3266833-3266852 MsG0480018317.01.T01:intron 30.0%
!! ATCTCTGAAGTCTCTTAATT+TGG - Chr4:3266451-3266470 MsG0480018317.01.T01:intron 30.0%
!! ATTTACCTTGGTTCTTTTAC+AGG - Chr4:3264879-3264898 MsG0480018317.01.T01:intron 30.0%
!! TCTGATCATTTTTCCTATTG+GGG - Chr4:3266055-3266074 MsG0480018317.01.T01:intron 30.0%
!! TCTTGTATGCTTTGTTTCAA+TGG - Chr4:3265261-3265280 MsG0480018317.01.T01:intron 30.0%
!! TTTATAATCACAAGTGCTGA+TGG - Chr4:3266167-3266186 MsG0480018317.01.T01:intron 30.0%
!!! TGGGATCTACATTTTGTTTT+TGG - Chr4:3264820-3264839 MsG0480018317.01.T01:intron 30.0%
AAAACTGTGGAGTTAATTGC+TGG - Chr4:3267926-3267945 MsG0480018317.01.T01:CDS 35.0%
AAACTGTGGAGTTAATTGCT+GGG - Chr4:3267927-3267946 MsG0480018317.01.T01:CDS 35.0%
AATGAGGATGTAATACCCAT+TGG - Chr4:3264624-3264643 MsG0480018317.01.T01:intron 35.0%
ACTTCTGGATATATTCCTTG+TGG - Chr4:3265972-3265991 MsG0480018317.01.T01:intron 35.0%
AGCAAATCAATCAGATTGGT+TGG + Chr4:3266737-3266756 None:intergenic 35.0%
AGGAGCTCAATTGGTATTAT+TGG + Chr4:3266776-3266795 None:intergenic 35.0%
AGGTACGTATTTCTCTTGTT+TGG + Chr4:3265584-3265603 None:intergenic 35.0%
AGTGGTTTAAGGAAATTGTG+TGG - Chr4:3266422-3266441 MsG0480018317.01.T01:intron 35.0%
ATCTAACCTTTCAATGGGAA+CGG + Chr4:3267018-3267037 None:intergenic 35.0%
ATGTGGTTTCTTATACCGTT+TGG - Chr4:3267331-3267350 MsG0480018317.01.T01:intron 35.0%
CACAGGAATAAAAGAGATTC+TGG - Chr4:3267051-3267070 MsG0480018317.01.T01:intron 35.0%
CACCAATCTTACATGAATCA+AGG + Chr4:3265187-3265206 None:intergenic 35.0%
CAGTAGCAAATCAATCAGAT+TGG + Chr4:3266741-3266760 None:intergenic 35.0%
CTAGACAATCCTACAAGAAA+TGG + Chr4:3266362-3266381 None:intergenic 35.0%
GAAATAGGAATAGAAATGGG+TGG + Chr4:3265614-3265633 None:intergenic 35.0%
GATACTGAAGTTGGAAACAA+TGG - Chr4:3265879-3265898 MsG0480018317.01.T01:CDS 35.0%
GATGAGTGAACTTTGTTGAT+TGG - Chr4:3264554-3264573 MsG0480018317.01.T01:intron 35.0%
GTTTCAATGGAGAAAACGAT+AGG - Chr4:3265274-3265293 MsG0480018317.01.T01:intron 35.0%
TAATTTCCGTTCCCATTGAA+AGG - Chr4:3267009-3267028 MsG0480018317.01.T01:intron 35.0%
TACTGCAAAGTGATTATCTG+TGG - Chr4:3267291-3267310 MsG0480018317.01.T01:intron 35.0%
TAGCTCATTTGAAAGGTGAT+TGG - Chr4:3264958-3264977 MsG0480018317.01.T01:intron 35.0%
TCAAACCTGATAAATGCTCT+AGG + Chr4:3265905-3265924 None:intergenic 35.0%
TCATAAGCAATTGATCTGCT+TGG - Chr4:3265849-3265868 MsG0480018317.01.T01:CDS 35.0%
TCTGGATGAAGTATATTCCT+GGG - Chr4:3267069-3267088 MsG0480018317.01.T01:intron 35.0%
TGGGAATCTAACCTTTCAAT+GGG + Chr4:3267023-3267042 None:intergenic 35.0%
TGGTAAAATCAAGTGAGAGT+GGG - Chr4:3266958-3266977 MsG0480018317.01.T01:intron 35.0%
TGTCAGTCAGAAGAGATTAA+TGG + Chr4:3267603-3267622 None:intergenic 35.0%
TTCTGGATGAAGTATATTCC+TGG - Chr4:3267068-3267087 MsG0480018317.01.T01:intron 35.0%
TTCTTTGAGTCGTAGAAGTT+AGG - Chr4:3266263-3266282 MsG0480018317.01.T01:intron 35.0%
TTGTTAGTGACAGTGGTTTA+AGG - Chr4:3266411-3266430 MsG0480018317.01.T01:intron 35.0%
! AGATGCAATATTTCTGACAG+AGG - Chr4:3264713-3264732 MsG0480018317.01.T01:intron 35.0%
! GATGCAATATTTCTGACAGA+GGG - Chr4:3264714-3264733 MsG0480018317.01.T01:intron 35.0%
! GATTTGGGATAGGAAGAAAT+AGG + Chr4:3265629-3265648 None:intergenic 35.0%
! GCAAATCAATCAGATTGGTT+GGG + Chr4:3266736-3266755 None:intergenic 35.0%
! GCATGTTTAGCTCATTTGAA+AGG - Chr4:3264951-3264970 MsG0480018317.01.T01:intron 35.0%
!! AGCGTCAGTTTTAACCTTAT+GGG - Chr4:3265428-3265447 MsG0480018317.01.T01:intron 35.0%
!! GTTGATGGTAACATTTACCT+TGG - Chr4:3264867-3264886 MsG0480018317.01.T01:intron 35.0%
!! TAAGATTGGTGATGAAGGAT+TGG - Chr4:3265196-3265215 MsG0480018317.01.T01:intron 35.0%
!! TATTGGTAGTGATGACTTCT+TGG - Chr4:3265668-3265687 MsG0480018317.01.T01:intron 35.0%
!! TCAAATTAGCACCGACATAT+GGG + Chr4:3267747-3267766 None:intergenic 35.0%
!! TCAGTTTTAACCTTATGGGA+AGG - Chr4:3265432-3265451 MsG0480018317.01.T01:intron 35.0%
!! TCATGTAAGATTGGTGATGA+AGG - Chr4:3265191-3265210 MsG0480018317.01.T01:intron 35.0%
!! TGGATTAATGGATTTGGGAT+AGG + Chr4:3265639-3265658 None:intergenic 35.0%
!! TTCAAATTAGCACCGACATA+TGG + Chr4:3267748-3267767 None:intergenic 35.0%
!!! CATTTTGAATTTGCTGATGC+AGG - Chr4:3267168-3267187 MsG0480018317.01.T01:CDS 35.0%
!!! TGCTTCTCTCTCTGTTTTTT+GGG - Chr4:3266100-3266119 MsG0480018317.01.T01:intron 35.0%
!!! TGGAGAAAAGTTTTTTGGGT+GGG - Chr4:3265515-3265534 MsG0480018317.01.T01:intron 35.0%
!!! TGTCTCTTTTTTGCAATGCT+TGG - Chr4:3267464-3267483 MsG0480018317.01.T01:intron 35.0%
!!! TTGTTCAGCCTCTGTTTTTT+AGG - Chr4:3267119-3267138 MsG0480018317.01.T01:intron 35.0%
AAGCAGACATCAATGCCAAA+TGG + Chr4:3266085-3266104 None:intergenic 40.0%
AATTAACTGCATGCTACAGC+TGG - Chr4:3267708-3267727 MsG0480018317.01.T01:intron 40.0%
ACTCTGCTTGTTTGGATGAA+TGG - Chr4:3265373-3265392 MsG0480018317.01.T01:intron 40.0%
ACTGTCACTAACAACAGTGA+AGG + Chr4:3266405-3266424 None:intergenic 40.0%
AGAGTGGGAGAAGTTATAGT+TGG - Chr4:3266973-3266992 MsG0480018317.01.T01:intron 40.0%
AGATTCCCACACTTTATCAC+AGG - Chr4:3267034-3267053 MsG0480018317.01.T01:intron 40.0%
AGCAGAAGCCTAAAAAACAG+AGG + Chr4:3267130-3267149 None:intergenic 40.0%
AGCTGGCACATTATTGTACT+TGG - Chr4:3265724-3265743 MsG0480018317.01.T01:intron 40.0%
ATCACAGAACAGCAACCTTA+CGG - Chr4:3267901-3267920 MsG0480018317.01.T01:CDS 40.0%
ATGAGACGGACAATTTGACT+GGG + Chr4:3267574-3267593 None:intergenic 40.0%
ATGGGAACACATACTTGCAA+GGG + Chr4:3266510-3266529 None:intergenic 40.0%
CAAACAAATCAAGGTCAGTG+AGG + Chr4:3267203-3267222 None:intergenic 40.0%
CACTCCTTGAGTCATTAAAG+TGG - Chr4:3264473-3264492 MsG0480018317.01.T01:CDS 40.0%
CTCTCCACTTTAATGACTCA+AGG + Chr4:3264480-3264499 None:intergenic 40.0%
CTTGAGTCATTAAAGTGGAG+AGG - Chr4:3264478-3264497 MsG0480018317.01.T01:CDS 40.0%
GCAAGGGTTGGAGAAATAAA+AGG + Chr4:3264430-3264449 None:intergenic 40.0%
GCTTACCAAATTACTGATGC+TGG - Chr4:3266476-3266495 MsG0480018317.01.T01:intron 40.0%
GGATTGTCTGATACTGAAGT+TGG - Chr4:3265870-3265889 MsG0480018317.01.T01:CDS 40.0%
GGGTTGGAGAAATAAAAGGT+TGG + Chr4:3264426-3264445 None:intergenic 40.0%
GTGGGAATCTAACCTTTCAA+TGG + Chr4:3267024-3267043 None:intergenic 40.0%
GTGGTAAAATCAAGTGAGAG+TGG - Chr4:3266957-3266976 MsG0480018317.01.T01:intron 40.0%
TAGAAATGGGTGGGAAATAG+AGG + Chr4:3265604-3265623 None:intergenic 40.0%
TCTTCTTTCTCCTTCCCATA+AGG + Chr4:3265445-3265464 None:intergenic 40.0%
TCTTGTAGGATTGTCTAGTC+TGG - Chr4:3266364-3266383 MsG0480018317.01.T01:intron 40.0%
TGTCATTGCAAGTGAAAGTG+TGG - Chr4:3265799-3265818 MsG0480018317.01.T01:intron 40.0%
TTCACTGTTGTTAGTGACAG+TGG - Chr4:3266404-3266423 MsG0480018317.01.T01:intron 40.0%
! AATGGCCTAGAGCATTTATC+AGG - Chr4:3265897-3265916 MsG0480018317.01.T01:CDS 40.0%
! AGATTCAAGTTTGACAGTGC+AGG + Chr4:3264689-3264708 None:intergenic 40.0%
! ATTCAAGAATCTGAGGTCCT+TGG - Chr4:3267802-3267821 MsG0480018317.01.T01:intron 40.0%
! GATTCAAGTTTGACAGTGCA+GGG + Chr4:3264688-3264707 None:intergenic 40.0%
! TGATGAAGGATTGGTGAACT+TGG - Chr4:3265205-3265224 MsG0480018317.01.T01:intron 40.0%
! TTGGTTTGAGCTAAGGCTTA+AGG + Chr4:3266705-3266724 None:intergenic 40.0%
!! AGCTCAATTGGTTTGAGCTA+AGG + Chr4:3266712-3266731 None:intergenic 40.0%
!! CTCACTGACCTTGATTTGTT+TGG - Chr4:3267201-3267220 MsG0480018317.01.T01:intron 40.0%
!! GAGCGTCAGTTTTAACCTTA+TGG - Chr4:3265427-3265446 MsG0480018317.01.T01:intron 40.0%
!!! CTGCTTCTCTCTCTGTTTTT+TGG - Chr4:3266099-3266118 MsG0480018317.01.T01:intron 40.0%
!!! GTGGAGAAAAGTTTTTTGGG+TGG - Chr4:3265514-3265533 MsG0480018317.01.T01:intron 40.0%
AAGAACTTGTCAGGGAGAGA+TGG - Chr4:3266236-3266255 MsG0480018317.01.T01:intron 45.0%
AGGAGTGACAAGACAGTTTG+AGG + Chr4:3264460-3264479 None:intergenic 45.0%
AGGTCCTTGGAAATATGCAG+CGG - Chr4:3267815-3267834 MsG0480018317.01.T01:intron 45.0%
AGGTCGAGATTGCATGTGTT+GGG - Chr4:3265318-3265337 MsG0480018317.01.T01:intron 45.0%
CAACCTTACGGACAAAACTG+TGG - Chr4:3267913-3267932 MsG0480018317.01.T01:CDS 45.0%
CATGAGACGGACAATTTGAC+TGG + Chr4:3267575-3267594 None:intergenic 45.0%
CATGGGAACACATACTTGCA+AGG + Chr4:3266511-3266530 None:intergenic 45.0%
CCAAACCCATTCATTCCACA+AGG + Chr4:3265990-3266009 None:intergenic 45.0%
CCAAATTACTGATGCTGGAC+TGG - Chr4:3266481-3266500 MsG0480018317.01.T01:intron 45.0%
CCAATTGAGCTCCTGTTTCA+AGG - Chr4:3266782-3266801 MsG0480018317.01.T01:intron 45.0%
CCAGTCCAGCATCAGTAATT+TGG + Chr4:3266484-3266503 None:intergenic 45.0%
CCAGTCCTGTAAAAGAACCA+AGG + Chr4:3264887-3264906 None:intergenic 45.0%
CCTTGAAACAGGAGCTCAAT+TGG + Chr4:3266785-3266804 None:intergenic 45.0%
GACAATTTGACTGGGACAGA+GGG + Chr4:3267566-3267585 None:intergenic 45.0%
GGAGTGACAAGACAGTTTGA+GGG + Chr4:3264459-3264478 None:intergenic 45.0%
GGCTCCGTAACAGTTATTGT+AGG - Chr4:3264841-3264860 MsG0480018317.01.T01:intron 45.0%
TAGGTCGAGATTGCATGTGT+TGG - Chr4:3265317-3265336 MsG0480018317.01.T01:intron 45.0%
TCCTAGCGCCAAACAAATCA+AGG + Chr4:3267212-3267231 None:intergenic 45.0%
TGAGCTAAGGCTTAAGGAGT+TGG + Chr4:3266699-3266718 None:intergenic 45.0%
TGCTCCGAAAGAGAAACTTC+TGG - Chr4:3265957-3265976 MsG0480018317.01.T01:CDS 45.0%
TTCTCTCGACTCTGCTTGTT+TGG - Chr4:3265365-3265384 MsG0480018317.01.T01:intron 45.0%
TTTGCTGATGCAGGTTTGAC+TGG - Chr4:3267177-3267196 MsG0480018317.01.T01:intron 45.0%
! CCTTGTGGAATGAATGGGTT+TGG - Chr4:3265987-3266006 MsG0480018317.01.T01:intron 45.0%
! GATTGGTTGGGTAGCTCAAT+TGG + Chr4:3266724-3266743 None:intergenic 45.0%
! TTTCTGACAGAGGGTGTGAT+AGG - Chr4:3264723-3264742 MsG0480018317.01.T01:intron 45.0%
!! ACCTTGATTTGTTTGGCGCT+AGG - Chr4:3267208-3267227 MsG0480018317.01.T01:intron 45.0%
!! ACTCCACAGTTTTGTCCGTA+AGG + Chr4:3267919-3267938 None:intergenic 45.0%
!! CCTTGGTTCTTTTACAGGAC+TGG - Chr4:3264884-3264903 MsG0480018317.01.T01:intron 45.0%
CTTAAGGAGTTGGAGGTCCT+GGG + Chr4:3266689-3266708 None:intergenic 50.0%
GATTGCATGTGTTGGGGGAT+AGG - Chr4:3265325-3265344 MsG0480018317.01.T01:intron 50.0%
GCGCTAGGATCACAGATGTT+GGG - Chr4:3267223-3267242 MsG0480018317.01.T01:intron 50.0%
GCTAAGGCTTAAGGAGTTGG+AGG + Chr4:3266696-3266715 None:intergenic 50.0%
GGACAATTTGACTGGGACAG+AGG + Chr4:3267567-3267586 None:intergenic 50.0%
GGTCGAGATTGCATGTGTTG+GGG - Chr4:3265319-3265338 MsG0480018317.01.T01:intron 50.0%
GTCGAGATTGCATGTGTTGG+GGG - Chr4:3265320-3265339 MsG0480018317.01.T01:intron 50.0%
GTGACAAGACAGTTTGAGGG+AGG + Chr4:3264456-3264475 None:intergenic 50.0%
TCCACCGCTGCATATTTCCA+AGG + Chr4:3267822-3267841 None:intergenic 50.0%
TCCTATTGGGGCTCACCATT+TGG - Chr4:3266067-3266086 MsG0480018317.01.T01:intron 50.0%
TCCTTGGAAATATGCAGCGG+TGG - Chr4:3267818-3267837 MsG0480018317.01.T01:intron 50.0%
TGTGTTGGGGGATAGGCATA+AGG - Chr4:3265332-3265351 MsG0480018317.01.T01:intron 50.0%
! AGTTTGAGGGAGGAGTGCAA+GGG + Chr4:3264446-3264465 None:intergenic 50.0%
! GAAGGATTGGTGAACTTGGC+AGG - Chr4:3265209-3265228 MsG0480018317.01.T01:intron 50.0%
CCTGGGTTCAAACCCGGAAA+AGG + Chr4:3266672-3266691 None:intergenic 55.0%
GCCAAATGGTGAGCCCCAAT+AGG + Chr4:3266071-3266090 None:intergenic 55.0%
GCTTAAGGAGTTGGAGGTCC+TGG + Chr4:3266690-3266709 None:intergenic 55.0%
GGCGCTAGGATCACAGATGT+TGG - Chr4:3267222-3267241 MsG0480018317.01.T01:intron 55.0%
TTTGACAGTGCAGGGAGCTC+TGG + Chr4:3264680-3264699 None:intergenic 55.0%
! CAGTTTGAGGGAGGAGTGCA+AGG + Chr4:3264447-3264466 None:intergenic 55.0%
! CCTTTTCCGGGTTTGAACCC+AGG - Chr4:3266669-3266688 MsG0480018317.01.T01:intron 55.0%
!! AGCGGTGGATTGACTGATGC+TGG - Chr4:3267833-3267852 MsG0480018317.01.T01:intron 55.0%
GGAGGTCCTGGGTTCAAACC+CGG + Chr4:3266678-3266697 None:intergenic 60.0%
! TGAGGGAGGAGTGCAAGGGT+TGG + Chr4:3264442-3264461 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 3264400 3267952 3264400 ID=MsG0480018317.01;Name=MsG0480018317.01
Chr4 mRNA 3264400 3267952 3264400 ID=MsG0480018317.01.T01;Parent=MsG0480018317.01;Name=MsG0480018317.01.T01;_AED=0.50;_eAED=0.60;_QI=0|0.66|0.57|1|0.83|0.71|7|0|249
Chr4 exon 3267838 3267952 3267838 ID=MsG0480018317.01.T01:exon:1379;Parent=MsG0480018317.01.T01
Chr4 exon 3267600 3267722 3267600 ID=MsG0480018317.01.T01:exon:1378;Parent=MsG0480018317.01.T01
Chr4 exon 3267380 3267451 3267380 ID=MsG0480018317.01.T01:exon:1377;Parent=MsG0480018317.01.T01
Chr4 exon 3267122 3267193 3267122 ID=MsG0480018317.01.T01:exon:1376;Parent=MsG0480018317.01.T01
Chr4 exon 3265837 3265980 3265837 ID=MsG0480018317.01.T01:exon:1375;Parent=MsG0480018317.01.T01
Chr4 exon 3265091 3265162 3265091 ID=MsG0480018317.01.T01:exon:1374;Parent=MsG0480018317.01.T01
Chr4 exon 3264400 3264551 3264400 ID=MsG0480018317.01.T01:exon:1373;Parent=MsG0480018317.01.T01
Chr4 CDS 3267838 3267952 3267838 ID=MsG0480018317.01.T01:cds;Parent=MsG0480018317.01.T01
Chr4 CDS 3267600 3267722 3267600 ID=MsG0480018317.01.T01:cds;Parent=MsG0480018317.01.T01
Chr4 CDS 3267380 3267451 3267380 ID=MsG0480018317.01.T01:cds;Parent=MsG0480018317.01.T01
Chr4 CDS 3267122 3267193 3267122 ID=MsG0480018317.01.T01:cds;Parent=MsG0480018317.01.T01
Chr4 CDS 3265837 3265980 3265837 ID=MsG0480018317.01.T01:cds;Parent=MsG0480018317.01.T01
Chr4 CDS 3265091 3265162 3265091 ID=MsG0480018317.01.T01:cds;Parent=MsG0480018317.01.T01
Chr4 CDS 3264400 3264551 3264400 ID=MsG0480018317.01.T01:cds;Parent=MsG0480018317.01.T01
Gene Sequence

>MsG0480018317.01.T01

ATGGTAGTCGGGGAAGTTGAACACCAACCTTTTATTTCTCCAACCCTTGCACTCCTCCCTCAAACTGTCTTGTCACTCCTTGAGTCATTAAAGTGGAGAGGTTTCAGTTTTACAAGATGTAATACCCATTGGAGTTTTGATTTAATATTGTTCTGTATTTTGCCAGAGCTCCCTGCACTGTCAAACTTGAATCTTAATAGATGCAATATTTCTGACAGAGGGTGTGATAGGTTTTCAAGACTGGAAAAGTTGAAAGTATTAAACTTGGGATTTAATGACATAGGAGATGCATGTTTAGCTCATTTGAAAGGATTGACAAAATTGGAGAGCTTGAACCTTGATTCATGTAAGATTGGTGATGAAGGATTGGTGAACTTGGCAGGATTGTCTAGTCTGGAGAAAATAAATCTGTCCTTCACTGTTGTTAGTGACAGTGGTTTAAGGAAATTGTGTGGACTCTCATCTCTGAAGTCTCTTAATTTGGATGCTTACCAAATTACTGATGCTGGACTGGCAACCCTTGCAAGTTTGACTGGCCTCACTGACCTTGATTTGTTTGGCGCTAGGATCACAGATGTTGGGACAAATTATTTGAAAAAATTCAAGAATCTGAGGTCCTTGGAAATATGCAGCGGTGGATTGACTGATGCTGGTTGTGAAAATATCAAAGAGCTTTCTTCTCTAATGTGCTTAAATTTATCACAGAACAGCAACCTTACGGACAAAACTGTGGAGTTAATTGCTGGGTAA
Protein sequence

>MsG0480018317.01.T01

MVVGEVEHQPFISPTLALLPQTVLSLLESLKWRGFSFTRCNTHWSFDLILFCILPELPALSNLNLNRCNISDRGCDRFSRLEKLKVLNLGFNDIGDACLAHLKGLTKLESLNLDSCKIGDEGLVNLAGLSSLEKINLSFTVVSDSGLRKLCGLSSLKSLNLDAYQITDAGLATLASLTGLTDLDLFGARITDVGTNYLKKFKNLRSLEICSGGLTDAGCENIKELSSLMCLNLSQNSNLTDKTVELIAG*