AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022113.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022113.01.T01 MTR_4g082330 91.250 160 13 1 1 159 1 160 3.71e-83 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTGAGGGTGGCTTCTA+AGG 0.266560 4:-68380441 MsG0480022113.01.T01:CDS
ACCGCGTTGCAGCCCGCGTC+GGG 0.276137 4:+68380525 None:intergenic
GGCTGCAACGCGGTTGGATT+GGG 0.277741 4:-68380516 MsG0480022113.01.T01:CDS
AAACAACAATATTGAAGTTC+AGG 0.324417 4:-68380660 MsG0480022113.01.T01:CDS
GGGCTGCAACGCGGTTGGAT+TGG 0.330424 4:-68380517 MsG0480022113.01.T01:CDS
AAAGAAGAAATAATAATTTC+CGG 0.352221 4:+68380251 None:intergenic
TGGTCAATTACGTGCGTGGC+TGG 0.395524 4:-68380386 MsG0480022113.01.T01:CDS
ATTCCGAACCGTCCACCTTC+CGG 0.399012 4:+68380312 None:intergenic
CGTAACTATGCAATGTGTGC+GGG 0.429868 4:-68380416 MsG0480022113.01.T01:CDS
ACGGTTCGGAATATCTTCTT+AGG 0.438601 4:-68380301 MsG0480022113.01.T01:CDS
AGGAAATTCCTTCTTCATGA+TGG 0.453632 4:+68380594 None:intergenic
TCGCCGGAAGGTGGACGGTT+CGG 0.463920 4:-68380315 MsG0480022113.01.T01:CDS
AACCGCGTTGCAGCCCGCGT+CGG 0.468668 4:+68380524 None:intergenic
TGAAATGAATAAGACGTTGA+AGG 0.469901 4:-68380489 MsG0480022113.01.T01:CDS
TGTAGCTGTGACGGCGGTGT+TGG 0.471963 4:-68380360 MsG0480022113.01.T01:CDS
CGGCGGTGTTGGTTTACGTC+GGG 0.474783 4:-68380349 MsG0480022113.01.T01:CDS
GACGCGGGCTGCAACGCGGT+TGG 0.485105 4:-68380522 MsG0480022113.01.T01:CDS
TGGTTTACGTCGGGATTCAA+AGG 0.489575 4:-68380340 MsG0480022113.01.T01:CDS
GAAGAAGAAGAACAAGAAAG+AGG 0.493181 4:+68380626 None:intergenic
AACGGAACTTGACGCCGTCA+TGG 0.509958 4:+68380568 None:intergenic
TGAGGTGAGTATATGGCATC+CGG 0.515752 4:-68380270 MsG0480022113.01.T01:CDS
TTCACCTTCCTCGCCACCAA+CGG 0.518986 4:+68380550 None:intergenic
CACACCACTCTCGAATCCTC+TGG 0.521436 4:-68380707 MsG0480022113.01.T01:CDS
TCGGGATTCAAAGGCGTCGC+CGG 0.522229 4:-68380331 MsG0480022113.01.T01:CDS
ACGGCGTCAAGTTCCGTTGG+TGG 0.523122 4:-68380563 MsG0480022113.01.T01:CDS
AACAACAATATTGAAGTTCA+GGG 0.523883 4:-68380659 MsG0480022113.01.T01:CDS
ACGGAACTTGACGCCGTCAT+GGG 0.524511 4:+68380569 None:intergenic
AGTTCCGTTGGTGGCGAGGA+AGG 0.531811 4:-68380554 MsG0480022113.01.T01:CDS
GTCAAGTTCCGTTGGTGGCG+AGG 0.531974 4:-68380558 MsG0480022113.01.T01:CDS
CCGCGTTGCAGCCCGCGTCG+GGG 0.537525 4:+68380526 None:intergenic
ACTTGACGCCGTCATGGGCG+AGG 0.539198 4:+68380574 None:intergenic
TCAAAGGCGTCGCCGGAAGG+TGG 0.547716 4:-68380324 MsG0480022113.01.T01:CDS
CCCCGACGCGGGCTGCAACG+CGG 0.549132 4:-68380526 MsG0480022113.01.T01:CDS
GGAAATTCCTTCTTCATGAT+GGG 0.551150 4:+68380595 None:intergenic
TTCTTCATGATGGGTAGGAG+TGG 0.563504 4:+68380604 None:intergenic
GGAAGGTGAAGCCCCGACGC+GGG 0.567663 4:-68380537 MsG0480022113.01.T01:CDS
GGCTGGAGCTGTAGCTGTGA+CGG 0.568693 4:-68380369 MsG0480022113.01.T01:CDS
ACTCCTACCCATCATGAAGA+AGG 0.569002 4:-68380602 MsG0480022113.01.T01:CDS
ATTCCTTCTTCATGATGGGT+AGG 0.572462 4:+68380599 None:intergenic
ATGACGGCGTCAAGTTCCGT+TGG 0.572657 4:-68380566 MsG0480022113.01.T01:CDS
TAAGACGTTGAAGGTGCGGA+TGG 0.572695 4:-68380480 MsG0480022113.01.T01:CDS
GATTCAAAGGCGTCGCCGGA+AGG 0.574232 4:-68380327 MsG0480022113.01.T01:CDS
AGGAATTTCCTCGCCCATGA+CGG 0.575563 4:-68380582 MsG0480022113.01.T01:CDS
ACGGCGGTGTTGGTTTACGT+CGG 0.576717 4:-68380350 MsG0480022113.01.T01:CDS
AGGCGTCGCCGGAAGGTGGA+CGG 0.577749 4:-68380320 MsG0480022113.01.T01:CDS
GTAACTATGCAATGTGTGCG+GGG 0.581011 4:-68380415 MsG0480022113.01.T01:CDS
GACGTTGAAGGTGCGGATGG+AGG 0.584100 4:-68380477 MsG0480022113.01.T01:CDS
CCACTCTCGAATCCTCTGGA+TGG 0.589063 4:-68380703 MsG0480022113.01.T01:CDS
GATTATTCTGTTCCATCCAG+AGG 0.593351 4:+68380691 None:intergenic
TGAATAAGACGTTGAAGGTG+CGG 0.597673 4:-68380484 MsG0480022113.01.T01:CDS
GCGGATGGAGGCTATGAGAG+TGG 0.619387 4:-68380465 MsG0480022113.01.T01:CDS
ACAATATTGAAGTTCAGGGA+GGG 0.623670 4:-68380655 MsG0480022113.01.T01:CDS
TCGTAACTATGCAATGTGTG+CGG 0.627278 4:-68380417 MsG0480022113.01.T01:CDS
CCATCCAGAGGATTCGAGAG+TGG 0.631254 4:+68380703 None:intergenic
TGGAGCTGTAGCTGTGACGG+CGG 0.631922 4:-68380366 MsG0480022113.01.T01:CDS
AACAATATTGAAGTTCAGGG+AGG 0.646038 4:-68380656 MsG0480022113.01.T01:CDS
TAACTATGCAATGTGTGCGG+GGG 0.646393 4:-68380414 MsG0480022113.01.T01:CDS
AGGAAGGTGAAGCCCCGACG+CGG 0.653291 4:-68380538 MsG0480022113.01.T01:CDS
CTATGAGAGTGGAAATTGTG+AGG 0.658550 4:-68380454 MsG0480022113.01.T01:CDS
GAGAGTGGAAATTGTGAGGG+TGG 0.678186 4:-68380450 MsG0480022113.01.T01:CDS
TATGAGAGTGGAAATTGTGA+GGG 0.682385 4:-68380453 MsG0480022113.01.T01:CDS
GCATGGAGGAAATAAACGAG+TGG 0.717528 4:-68380745 MsG0480022113.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAGAAATAATAATTTC+CGG + Chr4:68380736-68380755 None:intergenic 15.0%
! AAACAACAATATTGAAGTTC+AGG - Chr4:68380324-68380343 MsG0480022113.01.T01:CDS 25.0%
! AACAACAATATTGAAGTTCA+GGG - Chr4:68380325-68380344 MsG0480022113.01.T01:CDS 25.0%
AAAAAGATGAGGTGAGTATA+TGG - Chr4:68380707-68380726 MsG0480022113.01.T01:CDS 30.0%
TGAAATGAATAAGACGTTGA+AGG - Chr4:68380495-68380514 MsG0480022113.01.T01:CDS 30.0%
AACAATATTGAAGTTCAGGG+AGG - Chr4:68380328-68380347 MsG0480022113.01.T01:CDS 35.0%
ACAATATTGAAGTTCAGGGA+GGG - Chr4:68380329-68380348 MsG0480022113.01.T01:CDS 35.0%
AGGAAATTCCTTCTTCATGA+TGG + Chr4:68380393-68380412 None:intergenic 35.0%
GAAGAAGAAGAACAAGAAAG+AGG + Chr4:68380361-68380380 None:intergenic 35.0%
GGAAATTCCTTCTTCATGAT+GGG + Chr4:68380392-68380411 None:intergenic 35.0%
TATGAGAGTGGAAATTGTGA+GGG - Chr4:68380531-68380550 MsG0480022113.01.T01:CDS 35.0%
TCTTCTTAGGCAAAAAGATG+AGG - Chr4:68380696-68380715 MsG0480022113.01.T01:CDS 35.0%
CTATGAGAGTGGAAATTGTG+AGG - Chr4:68380530-68380549 MsG0480022113.01.T01:CDS 40.0%
GATTATTCTGTTCCATCCAG+AGG + Chr4:68380296-68380315 None:intergenic 40.0%
TCGTAACTATGCAATGTGTG+CGG - Chr4:68380567-68380586 MsG0480022113.01.T01:CDS 40.0%
TGAATAAGACGTTGAAGGTG+CGG - Chr4:68380500-68380519 MsG0480022113.01.T01:CDS 40.0%
! ACGGTTCGGAATATCTTCTT+AGG - Chr4:68380683-68380702 MsG0480022113.01.T01:CDS 40.0%
!! ATTCCTTCTTCATGATGGGT+AGG + Chr4:68380388-68380407 None:intergenic 40.0%
AATTGTGAGGGTGGCTTCTA+AGG - Chr4:68380543-68380562 MsG0480022113.01.T01:CDS 45.0%
ACTCCTACCCATCATGAAGA+AGG - Chr4:68380382-68380401 MsG0480022113.01.T01:CDS 45.0%
CGTAACTATGCAATGTGTGC+GGG - Chr4:68380568-68380587 MsG0480022113.01.T01:CDS 45.0%
GTAACTATGCAATGTGTGCG+GGG - Chr4:68380569-68380588 MsG0480022113.01.T01:CDS 45.0%
TAACTATGCAATGTGTGCGG+GGG - Chr4:68380570-68380589 MsG0480022113.01.T01:CDS 45.0%
TGAGGTGAGTATATGGCATC+CGG - Chr4:68380714-68380733 MsG0480022113.01.T01:CDS 45.0%
TGGTTTACGTCGGGATTCAA+AGG - Chr4:68380644-68380663 MsG0480022113.01.T01:CDS 45.0%
!! GTTTTGGTCAATTACGTGCG+TGG - Chr4:68380594-68380613 MsG0480022113.01.T01:CDS 45.0%
!! TTCTTCATGATGGGTAGGAG+TGG + Chr4:68380383-68380402 None:intergenic 45.0%
AGGAATTTCCTCGCCCATGA+CGG - Chr4:68380402-68380421 MsG0480022113.01.T01:CDS 50.0%
GAGAGTGGAAATTGTGAGGG+TGG - Chr4:68380534-68380553 MsG0480022113.01.T01:CDS 50.0%
! TAAGACGTTGAAGGTGCGGA+TGG - Chr4:68380504-68380523 MsG0480022113.01.T01:CDS 50.0%
AACGGAACTTGACGCCGTCA+TGG + Chr4:68380419-68380438 None:intergenic 55.0%
ACGGAACTTGACGCCGTCAT+GGG + Chr4:68380418-68380437 None:intergenic 55.0%
ATGACGGCGTCAAGTTCCGT+TGG - Chr4:68380418-68380437 MsG0480022113.01.T01:CDS 55.0%
ATTCCGAACCGTCCACCTTC+CGG + Chr4:68380675-68380694 None:intergenic 55.0%
CACACCACTCTCGAATCCTC+TGG - Chr4:68380277-68380296 MsG0480022113.01.T01:CDS 55.0%
CCACTCTCGAATCCTCTGGA+TGG - Chr4:68380281-68380300 MsG0480022113.01.T01:CDS 55.0%
CCATCCAGAGGATTCGAGAG+TGG + Chr4:68380284-68380303 None:intergenic 55.0%
TGGTCAATTACGTGCGTGGC+TGG - Chr4:68380598-68380617 MsG0480022113.01.T01:CDS 55.0%
TTCACCTTCCTCGCCACCAA+CGG + Chr4:68380437-68380456 None:intergenic 55.0%
!! ACGGCGGTGTTGGTTTACGT+CGG - Chr4:68380634-68380653 MsG0480022113.01.T01:CDS 55.0%
ACGGCGTCAAGTTCCGTTGG+TGG - Chr4:68380421-68380440 MsG0480022113.01.T01:CDS 60.0%
GCGGATGGAGGCTATGAGAG+TGG - Chr4:68380519-68380538 MsG0480022113.01.T01:CDS 60.0%
GGCTGCAACGCGGTTGGATT+GGG - Chr4:68380468-68380487 MsG0480022113.01.T01:CDS 60.0%
GGCTGGAGCTGTAGCTGTGA+CGG - Chr4:68380615-68380634 MsG0480022113.01.T01:CDS 60.0%
TGGAGCTGTAGCTGTGACGG+CGG - Chr4:68380618-68380637 MsG0480022113.01.T01:CDS 60.0%
TGTAGCTGTGACGGCGGTGT+TGG - Chr4:68380624-68380643 MsG0480022113.01.T01:CDS 60.0%
! GACGTTGAAGGTGCGGATGG+AGG - Chr4:68380507-68380526 MsG0480022113.01.T01:CDS 60.0%
!! AGTTCCGTTGGTGGCGAGGA+AGG - Chr4:68380430-68380449 MsG0480022113.01.T01:CDS 60.0%
!! CGGCGGTGTTGGTTTACGTC+GGG - Chr4:68380635-68380654 MsG0480022113.01.T01:CDS 60.0%
!! GATTCAAAGGCGTCGCCGGA+AGG - Chr4:68380657-68380676 MsG0480022113.01.T01:CDS 60.0%
!! GTCAAGTTCCGTTGGTGGCG+AGG - Chr4:68380426-68380445 MsG0480022113.01.T01:CDS 60.0%
!! TCGGGATTCAAAGGCGTCGC+CGG - Chr4:68380653-68380672 MsG0480022113.01.T01:CDS 60.0%
!!! CAATGTGTGCGGGGGCGTTT+TGG - Chr4:68380578-68380597 MsG0480022113.01.T01:CDS 60.0%
ACTTGACGCCGTCATGGGCG+AGG + Chr4:68380413-68380432 None:intergenic 65.0%
AGGAAGGTGAAGCCCCGACG+CGG - Chr4:68380446-68380465 MsG0480022113.01.T01:CDS 65.0%
GGGCTGCAACGCGGTTGGAT+TGG - Chr4:68380467-68380486 MsG0480022113.01.T01:CDS 65.0%
TCGCCGGAAGGTGGACGGTT+CGG - Chr4:68380669-68380688 MsG0480022113.01.T01:CDS 65.0%
!! TCAAAGGCGTCGCCGGAAGG+TGG - Chr4:68380660-68380679 MsG0480022113.01.T01:CDS 65.0%
AACCGCGTTGCAGCCCGCGT+CGG + Chr4:68380463-68380482 None:intergenic 70.0%
GGAAGGTGAAGCCCCGACGC+GGG - Chr4:68380447-68380466 MsG0480022113.01.T01:CDS 70.0%
! AGGCGTCGCCGGAAGGTGGA+CGG - Chr4:68380664-68380683 MsG0480022113.01.T01:CDS 70.0%
ACCGCGTTGCAGCCCGCGTC+GGG + Chr4:68380462-68380481 None:intergenic 75.0%
! GACGCGGGCTGCAACGCGGT+TGG - Chr4:68380462-68380481 MsG0480022113.01.T01:CDS 75.0%
! CCGCGTTGCAGCCCGCGTCG+GGG + Chr4:68380461-68380480 None:intergenic 80.0%
!! CCCCGACGCGGGCTGCAACG+CGG - Chr4:68380458-68380477 MsG0480022113.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 68380241 68380765 68380241 ID=MsG0480022113.01;Name=MsG0480022113.01
Chr4 mRNA 68380241 68380765 68380241 ID=MsG0480022113.01.T01;Parent=MsG0480022113.01;Name=MsG0480022113.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|174
Chr4 exon 68380241 68380765 68380241 ID=MsG0480022113.01.T01:exon:31115;Parent=MsG0480022113.01.T01
Chr4 CDS 68380241 68380765 68380241 ID=MsG0480022113.01.T01:cds;Parent=MsG0480022113.01.T01
Gene Sequence

>MsG0480022113.01.T01

ATGGAGGAAATAAACGAGTGGCAAGAAATCGAACAACACACCACTCTCGAATCCTCTGGATGGAACAGAATAATCATCAACGAAAACAACAATATTGAAGTTCAGGGAGGGAGTAATCCTCTTTCTTGTTCTTCTTCTTCCACTCCTACCCATCATGAAGAAGGAATTTCCTCGCCCATGACGGCGTCAAGTTCCGTTGGTGGCGAGGAAGGTGAAGCCCCGACGCGGGCTGCAACGCGGTTGGATTGGGCTAATGAAATGAATAAGACGTTGAAGGTGCGGATGGAGGCTATGAGAGTGGAAATTGTGAGGGTGGCTTCTAAGGTTCGTAACTATGCAATGTGTGCGGGGGCGTTTTGGTCAATTACGTGCGTGGCTGGAGCTGTAGCTGTGACGGCGGTGTTGGTTTACGTCGGGATTCAAAGGCGTCGCCGGAAGGTGGACGGTTCGGAATATCTTCTTAGGCAAAAAGATGAGGTGAGTATATGGCATCCGGAAATTATTATTTCTTCTTTTTACTTCTAA

Protein sequence

>MsG0480022113.01.T01

MEEINEWQEIEQHTTLESSGWNRIIINENNNIEVQGGSNPLSCSSSSTPTHHEEGISSPMTASSSVGGEEGEAPTRAATRLDWANEMNKTLKVRMEAMRVEIVRVASKVRNYAMCAGAFWSITCVAGAVAVTAVLVYVGIQRRRRKVDGSEYLLRQKDEVSIWHPEIIISSFYF*