AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047574.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047574.01.T01 MTR_4g130410 52.414 145 10 4 50 135 25 169 2.89e-33 116
MsG0880047574.01.T01 MTR_4g130410 100.000 41 0 0 50 90 25 65 7.86e-24 92.0
MsG0880047574.01.T01 MTR_2g104130 63.793 58 17 1 39 96 18 71 5.33e-17 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047574.01.T01 AT3G18430 57.377 61 26 0 36 96 11 71 1.19e-17 76.3
MsG0880047574.01.T01 AT3G18430 57.377 61 26 0 36 96 11 71 1.19e-17 76.3
MsG0880047574.01.T01 AT3G18430 57.377 61 26 0 36 96 11 71 1.19e-17 76.3

Find 69 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGAAACAGTTGGCAGTTT+GGG 0.222561 8:-88233450 MsG0880047574.01.T01:CDS
CATCAATCAATACCGAATTT+TGG 0.255918 8:+88233478 None:intergenic
TGTGGGTATATTATACTCTA+AGG 0.273959 8:+88230300 None:intergenic
CTCAGAAACAGTTGGCAGTT+TGG 0.282140 8:-88233451 MsG0880047574.01.T01:CDS
GAGAGGATTGACGGCGAATT+CGG 0.282242 8:+88233080 None:intergenic
CTCTCTGAGTCTGAGGGGTT+TGG 0.317578 8:-88233405 MsG0880047574.01.T01:CDS
TTTACAATACATTACATCTA+AGG 0.336187 8:+88230021 None:intergenic
CTGTTAGATTCTTGGCAATT+CGG 0.336457 8:-88230375 MsG0880047574.01.T01:intron
ATCTCTGTCATGCCTTACTT+TGG 0.337491 8:-88230273 MsG0880047574.01.T01:three_prime_UTR
AGAGGATTGACGGCGAATTC+GGG 0.339160 8:+88233081 None:intergenic
TTTCTTAACAGATGTTGATT+AGG 0.345451 8:+88229906 None:intergenic
AACAATGTTGTCAACAGAAT+TGG 0.363333 8:-88229877 MsG0880047574.01.T01:three_prime_UTR
TTTGTACTTACAGGAAGTTC+TGG 0.378499 8:-88231804 MsG0880047574.01.T01:CDS
TCTCTGTCATGCCTTACTTT+GGG 0.381730 8:-88230272 MsG0880047574.01.T01:three_prime_UTR
AGCAGATACGAAGAGCTAAA+TGG 0.386628 8:-88229980 MsG0880047574.01.T01:three_prime_UTR
AGAGGTTCCCGTGGATTAGA+AGG 0.391388 8:-88230336 MsG0880047574.01.T01:CDS
CATTACATCTAAGGAGGAAA+AGG 0.407808 8:+88230030 None:intergenic
GAGCCCATTGGGCCAAAATT+CGG 0.416739 8:-88233490 MsG0880047574.01.T01:CDS
GCATTGCAAAAGGAAGAAAT+AGG 0.427613 8:-88229958 MsG0880047574.01.T01:three_prime_UTR
CGCCGTCAATCCTCTCTCTC+AGG 0.441146 8:-88233073 MsG0880047574.01.T01:intron
TCAAAGTCAATGAGCCCATT+GGG 0.468824 8:-88233501 None:intergenic
ATTACATCTAAGGAGGAAAA+GGG 0.471552 8:+88230031 None:intergenic
CAGTCTCTCTCTGAGTCTGA+GGG 0.473243 8:-88233411 MsG0880047574.01.T01:CDS
GAAATAGGAAAACATGCTAA+AGG 0.479445 8:-88229943 MsG0880047574.01.T01:three_prime_UTR
GTCAAAGTCAATGAGCCCAT+TGG 0.480963 8:-88233502 None:intergenic
GAGCTAAATGGCATTGCAAA+AGG 0.481038 8:-88229968 MsG0880047574.01.T01:three_prime_UTR
AACTATTGAGTTTCGTTGGA+AGG 0.484803 8:-88230079 MsG0880047574.01.T01:three_prime_UTR
TGTTGTGGAGCCTTGAGCCT+TGG 0.485306 8:-88233375 MsG0880047574.01.T01:CDS
GCCTTACTTTGGGTTTGATG+AGG 0.489581 8:-88230262 MsG0880047574.01.T01:three_prime_UTR
TCAGTCTCTCTCTGAGTCTG+AGG 0.490978 8:-88233412 MsG0880047574.01.T01:CDS
CCCTCATCAAACCCAAAGTA+AGG 0.493673 8:+88230261 None:intergenic
AAATAGGAAAACATGCTAAA+GGG 0.499080 8:-88229942 MsG0880047574.01.T01:three_prime_UTR
GGGTTTGGAGTTGAATGTTG+TGG 0.499471 8:-88233390 MsG0880047574.01.T01:CDS
GACCTGAGAGAGAGGATTGA+CGG 0.500526 8:+88233071 None:intergenic
GTCTGACTTCATGAAGGTAC+TGG 0.500970 8:-88231732 MsG0880047574.01.T01:CDS
CCTTACTTTGGGTTTGATGA+GGG 0.503255 8:-88230261 MsG0880047574.01.T01:three_prime_UTR
TGGCAATTCGGATTTGAAGA+TGG 0.512548 8:-88230363 MsG0880047574.01.T01:CDS
GCACAGGTCCTTGAGGAAGC+TGG 0.517817 8:-88231782 MsG0880047574.01.T01:CDS
ATTGACGGCGAATTCGGGAA+TGG 0.526501 8:+88233086 None:intergenic
CTATCCTCCTTCTAATCCAC+GGG 0.526957 8:+88230329 None:intergenic
TGGCAACTATTGAGTTTCGT+TGG 0.546663 8:-88230083 MsG0880047574.01.T01:three_prime_UTR
TTGTATAACCAGCTTCCTCA+AGG 0.557492 8:+88231774 None:intergenic
AGTCTTGTCTGACTTCATGA+AGG 0.561332 8:-88231738 MsG0880047574.01.T01:CDS
CAGAAACAGTTGGCAGTTTG+GGG 0.566563 8:-88233449 MsG0880047574.01.T01:CDS
AAATTCGGTATTGATTGATG+TGG 0.567348 8:-88233475 MsG0880047574.01.T01:CDS
GCTATCCTCCTTCTAATCCA+CGG 0.570458 8:+88230328 None:intergenic
GAGGGAGAGACCTGAGAGAG+AGG 0.574191 8:+88233063 None:intergenic
CTCACTCACTCCAAGGCTCA+AGG 0.580773 8:+88233365 None:intergenic
TCGATCACTCACTCACTCCA+AGG 0.593233 8:+88233358 None:intergenic
GGGAATGGAAAGGAATTCAT+CGG 0.601066 8:+88233101 None:intergenic
ACAATACATTACATCTAAGG+AGG 0.604051 8:+88230024 None:intergenic
AATTCGGTATTGATTGATGT+GGG 0.604597 8:-88233474 MsG0880047574.01.T01:CDS
GGATTTGAAGATGGAAGCAG+AGG 0.611897 8:-88230354 MsG0880047574.01.T01:CDS
CGGCGAATTCGGGAATGGAA+AGG 0.615895 8:+88233091 None:intergenic
TGAGGAAGCTGGTTATACAA+AGG 0.621395 8:-88231771 MsG0880047574.01.T01:CDS
ATCTAAGGAGGAAAAGGGGT+AGG 0.624193 8:+88230036 None:intergenic
GATCGAGTTGACAGAAACGC+TGG 0.627666 8:+88233148 None:intergenic
CTTACAGGAAGTTCTGGCAC+AGG 0.635281 8:-88231798 MsG0880047574.01.T01:CDS
AGTTCTGGCACAGGTCCTTG+AGG 0.636503 8:-88231789 MsG0880047574.01.T01:CDS
GATGGAAGCAGAGGTTCCCG+TGG 0.647521 8:-88230345 MsG0880047574.01.T01:CDS
AGTCTCTCTCTGAGTCTGAG+GGG 0.648663 8:-88233410 MsG0880047574.01.T01:CDS
ATGGAAAGGAATTCATCGGA+AGG 0.655647 8:+88233105 None:intergenic
TAGGAAAACATGCTAAAGGG+AGG 0.656259 8:-88229939 MsG0880047574.01.T01:three_prime_UTR
CAACTCGATCGCAACAACTG+TGG 0.660071 8:-88233135 MsG0880047574.01.T01:CDS
AGAAACAGTTGGCAGTTTGG+GGG 0.660676 8:-88233448 MsG0880047574.01.T01:CDS
TTACATCTAAGGAGGAAAAG+GGG 0.669138 8:+88230032 None:intergenic
GGTTCCCGTGGATTAGAAGG+AGG 0.674884 8:-88230333 MsG0880047574.01.T01:CDS
GATGTGGGCTCAGAAACAGT+TGG 0.675310 8:-88233459 MsG0880047574.01.T01:CDS
ATATACTATCATTCACACTG+AGG 0.768906 8:+88230214 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAAAATATGTAAATC+AGG - Chr8:88231419-88231438 MsG0880047574.01.T01:intron 10.0%
!! AATATCTTAAACTTAAATAC+TGG + Chr8:88232167-88232186 None:intergenic 15.0%
!! ATATCTTAAACTTAAATACT+GGG + Chr8:88232166-88232185 None:intergenic 15.0%
!! TATATATGCTAAATCTAATT+AGG + Chr8:88232773-88232792 None:intergenic 15.0%
!! TGATTTGAGAAAAAAAAAAA+AGG + Chr8:88231946-88231965 None:intergenic 15.0%
!! TGCAATCATATATAAAATAT+AGG + Chr8:88231845-88231864 None:intergenic 15.0%
!! TTCATTAAATAAACTGATAA+AGG + Chr8:88231325-88231344 None:intergenic 15.0%
!! TTTAGCATATATAAACTTTA+TGG - Chr8:88232780-88232799 MsG0880047574.01.T01:intron 15.0%
!! TTTATCAGTTTATTTAATGA+AGG - Chr8:88231324-88231343 MsG0880047574.01.T01:intron 15.0%
!!! AATATCTTTATTTGAAACAT+AGG - Chr8:88232746-88232765 MsG0880047574.01.T01:intron 15.0%
!!! AATATGATTTTTTTAGCTAT+TGG - Chr8:88233259-88233278 MsG0880047574.01.T01:intron 15.0%
!!! TTTTTTTTGATACTTAAGTT+TGG - Chr8:88231690-88231709 MsG0880047574.01.T01:CDS 15.0%
!!! TTTTTTTTTTTCTCAAATCA+AGG - Chr8:88231946-88231965 MsG0880047574.01.T01:intron 15.0%
!! AAAATATAGGTAACTATTCT+GGG + Chr8:88231832-88231851 None:intergenic 20.0%
!! AACTATTTACTTCAAATACA+TGG - Chr8:88231797-88231816 MsG0880047574.01.T01:CDS 20.0%
!! TAAAAAAATGTATTGGAGAA+GGG + Chr8:88232515-88232534 None:intergenic 20.0%
!! TAAAATATAGGTAACTATTC+TGG + Chr8:88231833-88231852 None:intergenic 20.0%
!! TCTATAGTAAATAAGATGAA+AGG + Chr8:88230770-88230789 None:intergenic 20.0%
!! TTAGTATTTATGCAGTTTAA+CGG - Chr8:88232121-88232140 MsG0880047574.01.T01:intron 20.0%
!! TTTACAATACATTACATCTA+AGG + Chr8:88233349-88233368 None:intergenic 20.0%
!!! AAATGTCATGAGTTTTATTT+CGG - Chr8:88231016-88231035 MsG0880047574.01.T01:intron 20.0%
!!! AGTTTGGTAAACATTTTTAT+AGG - Chr8:88231706-88231725 MsG0880047574.01.T01:CDS 20.0%
!!! ATTTCCAAAATTGATTTGAA+AGG + Chr8:88230818-88230837 None:intergenic 20.0%
!!! TAATTTGAAGTAATTATCCA+TGG - Chr8:88231073-88231092 MsG0880047574.01.T01:intron 20.0%
! AAATAGGAAAACATGCTAAA+GGG - Chr8:88233425-88233444 MsG0880047574.01.T01:CDS 25.0%
! AAGGAACTCTAAATAGAAAT+TGG + Chr8:88230799-88230818 None:intergenic 25.0%
! AATAATCTTCTCAAGATCAA+TGG + Chr8:88232011-88232030 None:intergenic 25.0%
! ACCTAACTTATTTAGTAACT+AGG - Chr8:88232229-88232248 MsG0880047574.01.T01:intron 25.0%
! ATGAATGAGAAAAACCAAAA+AGG + Chr8:88231763-88231782 None:intergenic 25.0%
! CAAGAATCTAACAGTAAAAA+GGG + Chr8:88232986-88233005 None:intergenic 25.0%
! CCTATTTATGGAATTATACT+CGG - Chr8:88231157-88231176 MsG0880047574.01.T01:intron 25.0%
! CTAAAAAAATGTATTGGAGA+AGG + Chr8:88232516-88232535 None:intergenic 25.0%
! GTTAAACTGCATAAATACTA+AGG + Chr8:88232122-88232141 None:intergenic 25.0%
! GTTAAAGGAAATAGAAATGA+AGG + Chr8:88230150-88230169 None:intergenic 25.0%
! TAGTATTTATGCAGTTTAAC+GGG - Chr8:88232122-88232141 MsG0880047574.01.T01:intron 25.0%
! TGATTGCAAAACAAAAACTT+GGG - Chr8:88233178-88233197 MsG0880047574.01.T01:CDS 25.0%
! TGGAAAAATATGAAGGAAAA+TGG - Chr8:88232349-88232368 MsG0880047574.01.T01:intron 25.0%
! TTGATTGCAAAACAAAAACT+TGG - Chr8:88233177-88233196 MsG0880047574.01.T01:CDS 25.0%
! TTGGAAATTTGATGTTTCAA+GGG - Chr8:88230830-88230849 MsG0880047574.01.T01:intron 25.0%
! TTTCTGTTATGTGTATGAAT+TGG - Chr8:88230731-88230750 MsG0880047574.01.T01:intron 25.0%
! TTTGGAAATTTGATGTTTCA+AGG - Chr8:88230829-88230848 MsG0880047574.01.T01:intron 25.0%
!! AGTTCCTTTCAAATCAATTT+TGG - Chr8:88230811-88230830 MsG0880047574.01.T01:intron 25.0%
!! TAAACATTTTTATAGGCAAG+TGG - Chr8:88231713-88231732 MsG0880047574.01.T01:CDS 25.0%
!! TGCTTGCATAAATTGATAAT+TGG - Chr8:88230532-88230551 MsG0880047574.01.T01:intron 25.0%
!! TTTCTTAACAGATGTTGATT+AGG + Chr8:88233464-88233483 None:intergenic 25.0%
!!! ATTTTTTAAGTACTAGTCCA+CGG + Chr8:88230914-88230933 None:intergenic 25.0%
!!! GAAAAAAAAAGTATTAGGCA+GGG + Chr8:88232255-88232274 None:intergenic 25.0%
!!! GTTTTTCGTAGTTATTTTCA+AGG - Chr8:88230579-88230598 MsG0880047574.01.T01:intron 25.0%
!!! TCAATGACGTGTATTTTAAA+GGG + Chr8:88231275-88231294 None:intergenic 25.0%
AAAAGACAGTATGTAACCTT+GGG - Chr8:88231199-88231218 MsG0880047574.01.T01:intron 30.0%
AAAGCCTAAGTGTATTAGAA+AGG + Chr8:88232286-88232305 None:intergenic 30.0%
AACAATGTTGTCAACAGAAT+TGG - Chr8:88233490-88233509 MsG0880047574.01.T01:CDS 30.0%
AAGAAATAGAGGAAAATGAG+AGG + Chr8:88232597-88232616 None:intergenic 30.0%
AAGCCTAAGTGTATTAGAAA+GGG + Chr8:88232285-88232304 None:intergenic 30.0%
ACAATACATTACATCTAAGG+AGG + Chr8:88233346-88233365 None:intergenic 30.0%
ACAGAAAACGAAGAAGTTAA+AGG + Chr8:88230165-88230184 None:intergenic 30.0%
AGAAATAGAGGAAAATGAGA+GGG + Chr8:88232596-88232615 None:intergenic 30.0%
ATATACTATCATTCACACTG+AGG + Chr8:88233156-88233175 None:intergenic 30.0%
ATTACATCTAAGGAGGAAAA+GGG + Chr8:88233339-88233358 None:intergenic 30.0%
ATTGAGTGTGTGTGTATTAA+CGG - Chr8:88231290-88231309 MsG0880047574.01.T01:intron 30.0%
CATGACATTTCTCGTATTTA+CGG + Chr8:88231006-88231025 None:intergenic 30.0%
CCAAGAATCTAACAGTAAAA+AGG + Chr8:88232987-88233006 None:intergenic 30.0%
CCGAGTATAATTCCATAAAT+AGG + Chr8:88231160-88231179 None:intergenic 30.0%
GAAATAGGAAAACATGCTAA+AGG - Chr8:88233424-88233443 MsG0880047574.01.T01:CDS 30.0%
GAACAAATATCAACAGATAG+CGG + Chr8:88232926-88232945 None:intergenic 30.0%
GCCTAGTTACTAAATAAGTT+AGG + Chr8:88232233-88232252 None:intergenic 30.0%
GGGGAACTAAAAAAATGTAT+TGG + Chr8:88232522-88232541 None:intergenic 30.0%
TAAATACTGGGTCTGTTTAA+AGG + Chr8:88232154-88232173 None:intergenic 30.0%
TATGTCTGAACATCAAAGAA+AGG - Chr8:88230678-88230697 MsG0880047574.01.T01:intron 30.0%
TGAGAAAAAAAAAAAAGGCC+AGG + Chr8:88231941-88231960 None:intergenic 30.0%
TGTGGGTATATTATACTCTA+AGG + Chr8:88233070-88233089 None:intergenic 30.0%
TTTAACTCATCAGCAGTAAA+AGG + Chr8:88232718-88232737 None:intergenic 30.0%
! ACACACATTCAATTTTGTGT+TGG + Chr8:88230491-88230510 None:intergenic 30.0%
! ATGATATCTTGACAGTTTTG+AGG - Chr8:88230638-88230657 MsG0880047574.01.T01:intron 30.0%
! CATCAATCAATACCGAATTT+TGG + Chr8:88229892-88229911 None:intergenic 30.0%
! CCTTTTTACTGTTAGATTCT+TGG - Chr8:88232984-88233003 MsG0880047574.01.T01:intron 30.0%
! GTATTCTGTTTTGTACTTAC+AGG - Chr8:88231554-88231573 MsG0880047574.01.T01:intron 30.0%
! GTTTATGATACTGATTGCAA+TGG - Chr8:88230601-88230620 MsG0880047574.01.T01:intron 30.0%
! TTTGAAGTAATTATCCATGG+TGG - Chr8:88231076-88231095 MsG0880047574.01.T01:intron 30.0%
!! AAATTCGGTATTGATTGATG+TGG - Chr8:88229892-88229911 MsG0880047574.01.T01:three_prime_UTR 30.0%
!! AATACACTTAGGCTTTGTTT+GGG - Chr8:88232290-88232309 MsG0880047574.01.T01:intron 30.0%
!! AATTCGGTATTGATTGATGT+GGG - Chr8:88229893-88229912 MsG0880047574.01.T01:three_prime_UTR 30.0%
!! CTCAATGACGTGTATTTTAA+AGG + Chr8:88231276-88231295 None:intergenic 30.0%
!! GGAAAAAAAAAGTATTAGGC+AGG + Chr8:88232256-88232275 None:intergenic 30.0%
!! TAATACACTTAGGCTTTGTT+TGG - Chr8:88232289-88232308 MsG0880047574.01.T01:intron 30.0%
!! TATGTAAATCAGGTTGAGAA+TGG - Chr8:88231429-88231448 MsG0880047574.01.T01:intron 30.0%
!! TGATATCTTGACAGTTTTGA+GGG - Chr8:88230639-88230658 MsG0880047574.01.T01:intron 30.0%
!!! CCTAATACTTTTTTTTTCCC+CGG - Chr8:88232257-88232276 MsG0880047574.01.T01:intron 30.0%
!!! GATTTTTTTAGCTATTGGTG+TGG - Chr8:88233264-88233283 MsG0880047574.01.T01:intron 30.0%
!!! TATGGTTTTCTTTGAAACCT+AGG - Chr8:88232798-88232817 MsG0880047574.01.T01:intron 30.0%
AAATGTATTGGAGAAGGGTT+TGG + Chr8:88232510-88232529 None:intergenic 35.0%
AACTATTGAGTTTCGTTGGA+AGG - Chr8:88233288-88233307 MsG0880047574.01.T01:intron 35.0%
AATACAAAACCCTCCTCATT+TGG + Chr8:88232544-88232563 None:intergenic 35.0%
AATGTATTGGAGAAGGGTTT+GGG + Chr8:88232509-88232528 None:intergenic 35.0%
ATACAAAACCCTCCTCATTT+GGG + Chr8:88232543-88232562 None:intergenic 35.0%
ATATCATCCCTCGTTATTGA+GGG + Chr8:88231510-88231529 None:intergenic 35.0%
ATTATGTGAGGTGAGTTCTT+CGG + Chr8:88231123-88231142 None:intergenic 35.0%
CAAAAGACAGTATGTAACCT+TGG - Chr8:88231198-88231217 MsG0880047574.01.T01:intron 35.0%
CATTACATCTAAGGAGGAAA+AGG + Chr8:88233340-88233359 None:intergenic 35.0%
CTCATGCTGAATTTCTACTA+TGG - Chr8:88232889-88232908 MsG0880047574.01.T01:intron 35.0%
CTGTTAGATTCTTGGCAATT+CGG - Chr8:88232992-88233011 MsG0880047574.01.T01:intron 35.0%
GAAACAAGCCTATATCAGAT+AGG + Chr8:88231989-88232008 None:intergenic 35.0%
GAAATAGAGGAAAATGAGAG+GGG + Chr8:88232595-88232614 None:intergenic 35.0%
GAGAAAAACCAAAAAGGACT+TGG + Chr8:88231757-88231776 None:intergenic 35.0%
GCATTGCAAAAGGAAGAAAT+AGG - Chr8:88233409-88233428 MsG0880047574.01.T01:CDS 35.0%
GTTGAGAATGGAGACTTTAT+TGG - Chr8:88231441-88231460 MsG0880047574.01.T01:intron 35.0%
TAAATTGAGCTTAGAGCTGT+AGG - Chr8:88232096-88232115 MsG0880047574.01.T01:intron 35.0%
TATAGGCAAGTGGTAGATTA+GGG - Chr8:88231723-88231742 MsG0880047574.01.T01:CDS 35.0%
TGAACAATTTCCATACTCGA+TGG + Chr8:88231903-88231922 None:intergenic 35.0%
TGAGGGATGGAAAAATATGA+AGG - Chr8:88232342-88232361 MsG0880047574.01.T01:intron 35.0%
TTACATCTAAGGAGGAAAAG+GGG + Chr8:88233338-88233357 None:intergenic 35.0%
TTATAGGCAAGTGGTAGATT+AGG - Chr8:88231722-88231741 MsG0880047574.01.T01:CDS 35.0%
TTTGTACTTACAGGAAGTTC+TGG - Chr8:88231563-88231582 MsG0880047574.01.T01:intron 35.0%
! CACATAACAGAAAGCATAGA+AGG + Chr8:88230724-88230743 None:intergenic 35.0%
! CATACTCGTGTTATTATGTG+AGG + Chr8:88231135-88231154 None:intergenic 35.0%
!! CCGGGGAAAAAAAAAGTATT+AGG + Chr8:88232260-88232279 None:intergenic 35.0%
!!! GCATGACAGAGATTTTTTGT+GGG + Chr8:88233087-88233106 None:intergenic 35.0%
!!! TTTTTTAGTTCCCCCAAATG+AGG - Chr8:88232528-88232547 MsG0880047574.01.T01:intron 35.0%
AACCTAGGTCCTATTGTTTG+AGG - Chr8:88232813-88232832 MsG0880047574.01.T01:intron 40.0%
AACTCATCAGCAGTAAAAGG+CGG + Chr8:88232715-88232734 None:intergenic 40.0%
AAGTGTATTAGAAAGGGCTC+CGG + Chr8:88232279-88232298 None:intergenic 40.0%
ACCTAGGTCCTATTGTTTGA+GGG - Chr8:88232814-88232833 MsG0880047574.01.T01:intron 40.0%
AGATGAAAGGTGACAGTGAA+AGG + Chr8:88230757-88230776 None:intergenic 40.0%
AGCAGATACGAAGAGCTAAA+TGG - Chr8:88233387-88233406 MsG0880047574.01.T01:CDS 40.0%
ATCTCTGTCATGCCTTACTT+TGG - Chr8:88233094-88233113 MsG0880047574.01.T01:CDS 40.0%
ATGGAAAGGAATTCATCGGA+AGG + Chr8:88230265-88230284 None:intergenic 40.0%
ATGTATTGGAGAAGGGTTTG+GGG + Chr8:88232508-88232527 None:intergenic 40.0%
CACGAGTATGTGCCTATTTA+TGG - Chr8:88231145-88231164 MsG0880047574.01.T01:intron 40.0%
CATATCATCCCTCGTTATTG+AGG + Chr8:88231511-88231530 None:intergenic 40.0%
CTCAATAACGAGGGATGATA+TGG - Chr8:88231509-88231528 MsG0880047574.01.T01:intron 40.0%
GACAGTGACTTGATTGATCT+TGG + Chr8:88230979-88230998 None:intergenic 40.0%
GAGAATGGAGACTTTATTGG+AGG - Chr8:88231444-88231463 MsG0880047574.01.T01:intron 40.0%
GAGCCCTTTCTAATACACTT+AGG - Chr8:88232279-88232298 MsG0880047574.01.T01:intron 40.0%
GAGCTAAATGGCATTGCAAA+AGG - Chr8:88233399-88233418 MsG0880047574.01.T01:CDS 40.0%
GGAAAAAAGGAATGAGCAAG+TGG + Chr8:88232620-88232639 None:intergenic 40.0%
GGGAATGGAAAGGAATTCAT+CGG + Chr8:88230269-88230288 None:intergenic 40.0%
TACAAAACCCTCCTCATTTG+GGG + Chr8:88232542-88232561 None:intergenic 40.0%
TAGGAAAACATGCTAAAGGG+AGG - Chr8:88233428-88233447 MsG0880047574.01.T01:CDS 40.0%
TCAGAAACAGTTGGCAGTTT+GGG - Chr8:88229917-88229936 MsG0880047574.01.T01:three_prime_UTR 40.0%
TCGATTTATAGCAAGTAGCG+AGG + Chr8:88230342-88230361 None:intergenic 40.0%
TCTCTGTCATGCCTTACTTT+GGG - Chr8:88233095-88233114 MsG0880047574.01.T01:CDS 40.0%
TGAGCAAGTGGAAGAAATAG+AGG + Chr8:88232608-88232627 None:intergenic 40.0%
TGAGGAAGCTGGTTATACAA+AGG - Chr8:88231596-88231615 MsG0880047574.01.T01:intron 40.0%
TGATTGCACACATGATAGCT+TGG - Chr8:88231857-88231876 MsG0880047574.01.T01:intron 40.0%
TGGCAACTATTGAGTTTCGT+TGG - Chr8:88233284-88233303 MsG0880047574.01.T01:intron 40.0%
TGGCAATTCGGATTTGAAGA+TGG - Chr8:88233004-88233023 MsG0880047574.01.T01:intron 40.0%
TTGCATCACCCTCAAACAAT+AGG + Chr8:88232825-88232844 None:intergenic 40.0%
TTGTATAACCAGCTTCCTCA+AGG + Chr8:88231596-88231615 None:intergenic 40.0%
! AAGCTTTATCTCATCTCCCA+AGG + Chr8:88231218-88231237 None:intergenic 40.0%
! AGTCTTGTCTGACTTCATGA+AGG - Chr8:88231629-88231648 MsG0880047574.01.T01:intron 40.0%
! ATCATGCACCAAGTCCTTTT+TGG - Chr8:88231746-88231765 MsG0880047574.01.T01:CDS 40.0%
! CCTTACTTTGGGTTTGATGA+GGG - Chr8:88233106-88233125 MsG0880047574.01.T01:CDS 40.0%
! TCCTCTCTCGTGATTGTTTT+AGG - Chr8:88232850-88232869 MsG0880047574.01.T01:intron 40.0%
! TGTGTGTGTATTAACGGACA+GGG - Chr8:88231296-88231315 MsG0880047574.01.T01:intron 40.0%
!! AATACCGAATTTTGGCCCAA+TGG + Chr8:88229884-88229903 None:intergenic 40.0%
!! AGTTTGCTGAGAATGCTAGA+TGG - Chr8:88230408-88230427 MsG0880047574.01.T01:intron 40.0%
!! ATACCGAATTTTGGCCCAAT+GGG + Chr8:88229883-88229902 None:intergenic 40.0%
!! CAGCTCTAAGCTCAATTTAG+TGG + Chr8:88232095-88232114 None:intergenic 40.0%
!! CCTCTCTCGTGATTGTTTTA+GGG - Chr8:88232851-88232870 MsG0880047574.01.T01:intron 40.0%
!! GACGTGTATTTTAAAGGGAC+AGG + Chr8:88231270-88231289 None:intergenic 40.0%
!! GCTAGATGGCTTTAACTTCA+AGG - Chr8:88230422-88230441 MsG0880047574.01.T01:intron 40.0%
!! TTAGGCTTTGTTTGGGAGTT+TGG - Chr8:88232297-88232316 MsG0880047574.01.T01:intron 40.0%
!! TTTTAAGTACTAGTCCACGG+TGG + Chr8:88230911-88230930 None:intergenic 40.0%
!!! ACAGTTTTGAGGGATTTGAC+TGG - Chr8:88230649-88230668 MsG0880047574.01.T01:intron 40.0%
!!! GGCATGACAGAGATTTTTTG+TGG + Chr8:88233088-88233107 None:intergenic 40.0%
AAACAGAGCCTTAAGGCCAT+AGG - Chr8:88232663-88232682 MsG0880047574.01.T01:intron 45.0%
AACTCGCAAACAGAGCCTTA+AGG - Chr8:88232656-88232675 MsG0880047574.01.T01:intron 45.0%
AATCAAGGCCTGAGATTGAC+CGG - Chr8:88231961-88231980 MsG0880047574.01.T01:intron 45.0%
AATGTCGTACTGTGTGAGCA+TGG + Chr8:88230054-88230073 None:intergenic 45.0%
ACAAAACCCTCCTCATTTGG+GGG + Chr8:88232541-88232560 None:intergenic 45.0%
ACCCTCAAACAATAGGACCT+AGG + Chr8:88232818-88232837 None:intergenic 45.0%
ACTCATCAGCAGTAAAAGGC+GGG + Chr8:88232714-88232733 None:intergenic 45.0%
AGAAACAGTTGGCAGTTTGG+GGG - Chr8:88229919-88229938 MsG0880047574.01.T01:three_prime_UTR 45.0%
AGAAGGAGCAGGAGAAAGAT+TGG + Chr8:88230707-88230726 None:intergenic 45.0%
AGCCTATATCAGATAGGTCC+CGG + Chr8:88231983-88232002 None:intergenic 45.0%
AGGGGAAGACTTGGGAAAAA+AGG + Chr8:88232633-88232652 None:intergenic 45.0%
AGTGTATTAGAAAGGGCTCC+GGG + Chr8:88232278-88232297 None:intergenic 45.0%
ATAGAGGAAAATGAGAGGGG+AGG + Chr8:88232592-88232611 None:intergenic 45.0%
ATCTAAGGAGGAAAAGGGGT+AGG + Chr8:88233334-88233353 None:intergenic 45.0%
ATGATAGCTTGGAGTGACGA+GGG - Chr8:88231868-88231887 MsG0880047574.01.T01:intron 45.0%
CAGAAACAGTTGGCAGTTTG+GGG - Chr8:88229918-88229937 MsG0880047574.01.T01:three_prime_UTR 45.0%
CCCTAAAACAATCACGAGAG+AGG + Chr8:88232854-88232873 None:intergenic 45.0%
CCCTCATCAAACCCAAAGTA+AGG + Chr8:88233109-88233128 None:intergenic 45.0%
CGGTGGATCACTTATGAAGA+TGG + Chr8:88230894-88230913 None:intergenic 45.0%
CTATCCTCCTTCTAATCCAC+GGG + Chr8:88233041-88233060 None:intergenic 45.0%
CTCAGAAACAGTTGGCAGTT+TGG - Chr8:88229916-88229935 MsG0880047574.01.T01:three_prime_UTR 45.0%
GCTATCCTCCTTCTAATCCA+CGG + Chr8:88233042-88233061 None:intergenic 45.0%
GGATTTGAAGATGGAAGCAG+AGG - Chr8:88233013-88233032 MsG0880047574.01.T01:intron 45.0%
TAAGGCCATAGGTTTGTGAC+AGG - Chr8:88232674-88232693 MsG0880047574.01.T01:intron 45.0%
TAAGGCTCTGTTTGCGAGTT+TGG + Chr8:88232656-88232675 None:intergenic 45.0%
TACAGCCTGTCACAAACCTA+TGG + Chr8:88232682-88232701 None:intergenic 45.0%
TAGAGGAAAATGAGAGGGGA+GGG + Chr8:88232591-88232610 None:intergenic 45.0%
TCGATTGCCCTCAATAACGA+GGG - Chr8:88231500-88231519 MsG0880047574.01.T01:intron 45.0%
TCTTCATAAGTGATCCACCG+TGG - Chr8:88230894-88230913 MsG0880047574.01.T01:intron 45.0%
TGAGTGAGTGATCGATCGAT+GGG - Chr8:88230017-88230036 MsG0880047574.01.T01:three_prime_UTR 45.0%
TGTATTGGAGAAGGGTTTGG+GGG + Chr8:88232507-88232526 None:intergenic 45.0%
TTAGTTCCCCCAAATGAGGA+GGG - Chr8:88232532-88232551 MsG0880047574.01.T01:intron 45.0%
TTCGATTGCCCTCAATAACG+AGG - Chr8:88231499-88231518 MsG0880047574.01.T01:intron 45.0%
! ACAGAAAGCATAGAAGGAGC+AGG + Chr8:88230718-88230737 None:intergenic 45.0%
! CTTTGTTTGGGAGTTTGGAG+GGG - Chr8:88232302-88232321 MsG0880047574.01.T01:intron 45.0%
! GCCTTACTTTGGGTTTGATG+AGG - Chr8:88233105-88233124 MsG0880047574.01.T01:CDS 45.0%
! GGGTTTGGAGTTGAATGTTG+TGG - Chr8:88229977-88229996 MsG0880047574.01.T01:three_prime_UTR 45.0%
! GTCACAAACCTATGGCCTTA+AGG + Chr8:88232674-88232693 None:intergenic 45.0%
! GTCTGACTTCATGAAGGTAC+TGG - Chr8:88231635-88231654 MsG0880047574.01.T01:intron 45.0%
! GTGTGTGTGTATTAACGGAC+AGG - Chr8:88231295-88231314 MsG0880047574.01.T01:intron 45.0%
! TTTAGTTCCCCCAAATGAGG+AGG - Chr8:88232531-88232550 MsG0880047574.01.T01:intron 45.0%
!! GCTTTGTTTGGGAGTTTGGA+GGG - Chr8:88232301-88232320 MsG0880047574.01.T01:intron 45.0%
!!! AAAAAATGTTTAAAATAATA+CGG - Chr8:88230927-88230946 MsG0880047574.01.T01:intron 5.0%
!!! TATTTTTATTAACATTATTT+TGG + Chr8:88232201-88232220 None:intergenic 5.0%
AAAATGAGAGGGGAGGGCTT+TGG + Chr8:88232585-88232604 None:intergenic 50.0%
AAATGAGAGGGGAGGGCTTT+GGG + Chr8:88232584-88232603 None:intergenic 50.0%
AGAGGATTGACGGCGAATTC+GGG + Chr8:88230289-88230308 None:intergenic 50.0%
AGCGAGGAAGAGAAAGAGAG+AGG + Chr8:88230326-88230345 None:intergenic 50.0%
AGTCTCTCTCTGAGTCTGAG+GGG - Chr8:88229957-88229976 MsG0880047574.01.T01:three_prime_UTR 50.0%
ATCAAGGCCTGAGATTGACC+GGG - Chr8:88231962-88231981 MsG0880047574.01.T01:intron 50.0%
ATGTGAGGTGAGTTCTTCGG+TGG + Chr8:88231120-88231139 None:intergenic 50.0%
ATTGACGGCGAATTCGGGAA+TGG + Chr8:88230284-88230303 None:intergenic 50.0%
CAACTCGATCGCAACAACTG+TGG - Chr8:88230232-88230251 MsG0880047574.01.T01:three_prime_UTR 50.0%
CAGTCTCTCTCTGAGTCTGA+GGG - Chr8:88229956-88229975 MsG0880047574.01.T01:three_prime_UTR 50.0%
CATGATAGCTTGGAGTGACG+AGG - Chr8:88231867-88231886 MsG0880047574.01.T01:intron 50.0%
CCTTCATAAGTGGTCCACCA+TGG + Chr8:88231093-88231112 None:intergenic 50.0%
CTCTGTTTGCGAGTTTGGAG+GGG + Chr8:88232651-88232670 None:intergenic 50.0%
GACCGGGACCTATCTGATAT+AGG - Chr8:88231978-88231997 MsG0880047574.01.T01:intron 50.0%
GACCTGAGAGAGAGGATTGA+CGG + Chr8:88230299-88230318 None:intergenic 50.0%
GAGAGGATTGACGGCGAATT+CGG + Chr8:88230290-88230309 None:intergenic 50.0%
GAGCCCATTGGGCCAAAATT+CGG - Chr8:88229877-88229896 MsG0880047574.01.T01:three_prime_UTR 50.0%
GAGTTTGGAGGGGAAGACTT+GGG + Chr8:88232641-88232660 None:intergenic 50.0%
GATGTGGGCTCAGAAACAGT+TGG - Chr8:88229908-88229927 MsG0880047574.01.T01:three_prime_UTR 50.0%
GCGAGGAAGAGAAAGAGAGA+GGG + Chr8:88230325-88230344 None:intergenic 50.0%
GCTCTGTTTGCGAGTTTGGA+GGG + Chr8:88232652-88232671 None:intergenic 50.0%
GTATTGGAGAAGGGTTTGGG+GGG + Chr8:88232506-88232525 None:intergenic 50.0%
GTGAGTGAGTGATCGATCGA+TGG - Chr8:88230016-88230035 MsG0880047574.01.T01:three_prime_UTR 50.0%
GTGTATTAGAAAGGGCTCCG+GGG + Chr8:88232277-88232296 None:intergenic 50.0%
TATTGGAGAAGGGTTTGGGG+GGG + Chr8:88232505-88232524 None:intergenic 50.0%
TCAGCGAGACTAAACTGACC+TGG - Chr8:88231920-88231939 MsG0880047574.01.T01:intron 50.0%
TCAGTCTCTCTCTGAGTCTG+AGG - Chr8:88229955-88229974 MsG0880047574.01.T01:three_prime_UTR 50.0%
TCGATCACTCACTCACTCCA+AGG + Chr8:88230012-88230031 None:intergenic 50.0%
TTGTGTTGGAGAGTAGCACG+TGG + Chr8:88230477-88230496 None:intergenic 50.0%
! AGGGGAAGGAAAGGCTTTGA+GGG - Chr8:88232325-88232344 MsG0880047574.01.T01:intron 50.0%
! CTTACAGGAAGTTCTGGCAC+AGG - Chr8:88231569-88231588 MsG0880047574.01.T01:intron 50.0%
! TGGTGGACCACTTATGAAGG+TGG - Chr8:88231093-88231112 MsG0880047574.01.T01:intron 50.0%
!! AGAGGTTCCCGTGGATTAGA+AGG - Chr8:88233031-88233050 MsG0880047574.01.T01:intron 50.0%
!! CCATGGTGGACCACTTATGA+AGG - Chr8:88231090-88231109 MsG0880047574.01.T01:intron 50.0%
!! GAAGGAAAGGCTTTGAGGGA+TGG - Chr8:88232329-88232348 MsG0880047574.01.T01:intron 50.0%
!! GATCGAGTTGACAGAAACGC+TGG + Chr8:88230222-88230241 None:intergenic 50.0%
!! GGCTTTGTTTGGGAGTTTGG+AGG - Chr8:88232300-88232319 MsG0880047574.01.T01:intron 50.0%
AATGAGAGGGGAGGGCTTTG+GGG + Chr8:88232583-88232602 None:intergenic 55.0%
CGAGTTTGGAGGGGAAGACT+TGG + Chr8:88232642-88232661 None:intergenic 55.0%
CGGCGAATTCGGGAATGGAA+AGG + Chr8:88230279-88230298 None:intergenic 55.0%
CGGTGGACCACCTTCATAAG+TGG + Chr8:88231103-88231122 None:intergenic 55.0%
CTCACTCACTCCAAGGCTCA+AGG + Chr8:88230005-88230024 None:intergenic 55.0%
CTCTCTGAGTCTGAGGGGTT+TGG - Chr8:88229962-88229981 MsG0880047574.01.T01:three_prime_UTR 55.0%
GAGGGGAAGGAAAGGCTTTG+AGG - Chr8:88232324-88232343 MsG0880047574.01.T01:intron 55.0%
GATAGGTCCCGGTCAATCTC+AGG + Chr8:88231972-88231991 None:intergenic 55.0%
GGCTCTGTTTGCGAGTTTGG+AGG + Chr8:88232653-88232672 None:intergenic 55.0%
GTTTGGGAGTTTGGAGGGGA+GGG - Chr8:88232306-88232325 MsG0880047574.01.T01:intron 55.0%
TGTTGTGGAGCCTTGAGCCT+TGG - Chr8:88229992-88230011 MsG0880047574.01.T01:three_prime_UTR 55.0%
TGTTTGGGAGTTTGGAGGGG+AGG - Chr8:88232305-88232324 MsG0880047574.01.T01:intron 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG - Chr8:88232307-88232326 MsG0880047574.01.T01:intron 55.0%
! AGTTCTGGCACAGGTCCTTG+AGG - Chr8:88231578-88231597 MsG0880047574.01.T01:intron 55.0%
! GAAGGGTTTGGGGGGGATTT+TGG + Chr8:88232498-88232517 None:intergenic 55.0%
! GGTTTGGGGGGGATTTTGGA+GGG + Chr8:88232494-88232513 None:intergenic 55.0%
!! GGTTCCCGTGGATTAGAAGG+AGG - Chr8:88233034-88233053 MsG0880047574.01.T01:intron 55.0%
CGCCGTCAATCCTCTCTCTC+AGG - Chr8:88230294-88230313 MsG0880047574.01.T01:three_prime_UTR 60.0%
GAGGGAGAGACCTGAGAGAG+AGG + Chr8:88230307-88230326 None:intergenic 60.0%
GATGGAAGCAGAGGTTCCCG+TGG - Chr8:88233022-88233041 MsG0880047574.01.T01:intron 60.0%
GCACAGGTCCTTGAGGAAGC+TGG - Chr8:88231585-88231604 MsG0880047574.01.T01:intron 60.0%
TTGGAGGGGAGGGGAAGGAA+AGG - Chr8:88232316-88232335 MsG0880047574.01.T01:intron 60.0%
! ATGAGAGGGGAGGGCTTTGG+GGG + Chr8:88232582-88232601 None:intergenic 60.0%
! GGGTTTGGGGGGGATTTTGG+AGG + Chr8:88232495-88232514 None:intergenic 60.0%
!! GCGCTGACTGCCATCGAGTA+TGG - Chr8:88231890-88231909 MsG0880047574.01.T01:intron 60.0%
GGAGTTTGGAGGGGAGGGGA+AGG - Chr8:88232311-88232330 MsG0880047574.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 88229875 88233514 88229875 ID=MsG0880047574.01;Name=MsG0880047574.01
Chr8 mRNA 88229875 88233514 88229875 ID=MsG0880047574.01.T01;Parent=MsG0880047574.01;Name=MsG0880047574.01.T01;_AED=0.49;_eAED=0.61;_QI=0|0|0.5|1|0.33|0.5|4|454|165
Chr8 exon 88229875 88230390 88229875 ID=MsG0880047574.01.T01:exon:34838;Parent=MsG0880047574.01.T01
Chr8 exon 88231678 88231843 88231678 ID=MsG0880047574.01.T01:exon:34837;Parent=MsG0880047574.01.T01
Chr8 exon 88233074 88233198 88233074 ID=MsG0880047574.01.T01:exon:34836;Parent=MsG0880047574.01.T01
Chr8 exon 88233370 88233514 88233370 ID=MsG0880047574.01.T01:exon:34835;Parent=MsG0880047574.01.T01
Chr8 CDS 88233370 88233514 88233370 ID=MsG0880047574.01.T01:cds;Parent=MsG0880047574.01.T01
Chr8 CDS 88233074 88233198 88233074 ID=MsG0880047574.01.T01:cds;Parent=MsG0880047574.01.T01
Chr8 CDS 88231678 88231843 88231678 ID=MsG0880047574.01.T01:cds;Parent=MsG0880047574.01.T01
Chr8 CDS 88230329 88230390 88230329 ID=MsG0880047574.01.T01:cds;Parent=MsG0880047574.01.T01
Chr8 three_prime_UTR 88229875 88230328 88229875 ID=MsG0880047574.01.T01:three_prime_utr;Parent=MsG0880047574.01.T01
Gene Sequence

>MsG0880047574.01.T01

ATGAGCCCATTGGGCCAAAATTCGGTATTGATTGATGTGGGCTCAGAAACAGTTGGCAGTTTGGGGGAACTCGTGTTTCTTCAGTCTCTCTCTGAGTCTGAGGGGTTTGGAGTTGAATGTTGTGGAGCCTTGAGCCTTGGAGTGATTACTCAGCAAGAGATCGTTTCACTTTACCAGCGTTTCTGTCAACTCGATCGCAACAACTGTGGTTTCATTCCTTCCGATGAATTCCTTTCCATTCCCGAATTCGCCGTCAATCCTCTCTCTCAGCTTGTGATGTATTCTGTTTTGTACTTACAGGAAGTTCTGGCACAGGTCCTTGAGGAAGCTGGTTATACAAAGGATTCTTTCTTAGTCTTGTCTGACTTCATGAAGGTACTGGAACAAATAATAATATCTAGCATGATTTTTTTTTTTTTTTTGATACTTAAGTTTGATTCTTGGCAATTCGGATTTGAAGATGGAAGCAGAGGTTCCCGTGGATTAGAAGGAGGATAG
Protein sequence

>MsG0880047574.01.T01

MSPLGQNSVLIDVGSETVGSLGELVFLQSLSESEGFGVECCGALSLGVITQQEIVSLYQRFCQLDRNNCGFIPSDEFLSIPEFAVNPLSQLVMYSVLYLQEVLAQVLEEAGYTKDSFLVLSDFMKVLEQIIISSMIFFFFLILKFDSWQFGFEDGSRGSRGLEGG*