AlfalfaGEDB | Msa0047650

M. sativa cultivar zhongmu-4 / Msa0047650

KEGG: -
GO: GO:0000741 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0047650	sp\|Q93ZJ3\|NFD6_ARATH	60.000	70	26	1	21	90	28	95	6.02e-19	79.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0047650	Msa0194940	0.806828	6.804359e-50	-8.615850e-47
Msa0047650	Msa0340560	0.819088	1.395335e-52	-8.615850e-47
Msa0047650	Msa0432750	0.808701	2.721294e-50	-8.615850e-47
Msa0047650	Msa0479980	0.809386	1.941193e-50	-8.615850e-47
Msa0047650	Msa0544720	0.913017	1.001339e-83	-8.615850e-47
Msa0047650	Msa0608260	0.913728	4.391559e-84	-8.615850e-47
Msa0047650	Msa0691330	0.912565	1.684372e-83	-8.615850e-47
Msa0047650	Msa0930490	0.833603	4.850392e-56	-8.615850e-47
Msa0047650	Msa1083370	0.802261	6.099912e-49	-8.615850e-47
Msa0047650	Msa1106870	0.800012	1.759479e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0047650	MtrunA17_Chr4g0040461	95.556	90	4	0	1	90	1	90	1.61e-55	169
Msa0047650	MtrunA17_Chr2g0324851	71.910	89	23	1	1	89	4	90	2.71e-27	97.8
Msa0047650	MtrunA17_Chr2g0282671	47.674	86	42	2	4	89	3	85	3.74e-15	66.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0047650	AT2G20585.2	58.667	75	27	2	21	93	28	100	4.24e-20	80.1
Msa0047650	AT2G20585.5	60.000	70	26	1	21	90	28	95	6.12e-20	79.7
Msa0047650	AT2G20585.1	60.000	70	26	1	21	90	28	95	6.12e-20	79.7
Msa0047650	AT2G20585.4	58.571	70	27	1	21	90	28	95	1.58e-19	78.2
Msa0047650	AT2G20585.3	58.571	70	27	1	21	90	28	95	1.58e-19	78.2
Msa0047650	AT2G20585.6	58.571	70	27	1	21	90	28	95	2.25e-19	78.2
Msa0047650	AT2G20585.7	58.571	70	27	1	21	90	28	95	2.25e-19	78.2
Msa0047650	AT2G33847.1	54.762	84	31	3	4	83	2	82	9.82e-19	76.3
Msa0047650	AT2G33847.2	54.762	84	31	3	4	83	2	82	1.32e-18	75.9
Msa0047650	AT1G28395.2	51.579	95	36	4	4	92	2	92	2.04e-18	75.5

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Find 31 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCCACCAGAAAATCTATT+TGG	0.221185	1_1:+80729719	Msa0047650:CDS
AACTACGCTTGGCTTTCAGA+AGG	0.611860	1_1:+80727307	Msa0047650:CDS
AAGCGTAGTTGCGGCTTGAA+AGG	0.455777	1_1:-80727294	None:intergenic
ACCCACCAGAAAATCTATTT+GGG	0.449145	1_1:+80729720	Msa0047650:CDS
ACGAGCTGGTTTAGCTTCCG+AGG	0.599944	1_1:-80727040	None:intergenic
AGATGATCGGAACATTGATC+TGG	0.517704	1_1:-80726989	None:intergenic
AGCGTAGTTGCGGCTTGAAA+GGG	0.524369	1_1:-80727293	None:intergenic
AGCTCGTTCTCCGTTCCGAA+TGG	0.598424	1_1:+80727056	Msa0047650:CDS
AGCTTCCGAGGCGACACGGA+AGG	0.572335	1_1:-80727028	None:intergenic
ATCAGCCTTCCGTGTCGCCT+CGG	0.502584	1_1:+80727023	Msa0047650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACACAAAAACTCAATTCAAC+AGG	-	chr1_1:80727214-80727233	None:intergenic	30.0%
CAATGTGTTTATCTGCAATT+TGG	+	chr1_1:80727363-80727382	Msa0047650:intron	30.0%
ATATATGAGTTTATAAGCGG+AGG	+	chr1_1:80727570-80727589	Msa0047650:intron	30.0%
AAGATATCAAACAACATGAC+AGG	-	chr1_1:80727885-80727904	None:intergenic	30.0%
AAAAACAGAGGTAAACTAGT+AGG	-	chr1_1:80728027-80728046	None:intergenic	30.0%
TCAAAATTAAACCAATGGTC+AGG	-	chr1_1:80728055-80728074	None:intergenic	30.0%
TAAAAAGCTCAACAAATCAC+AGG	-	chr1_1:80728468-80728487	None:intergenic	30.0%
CTGTTGTCTGTTTGTATATT+AGG	+	chr1_1:80728917-80728936	Msa0047650:intron	30.0%
TATGCTCAATATTTACATGG+TGG	+	chr1_1:80729024-80729043	Msa0047650:intron	30.0%
GCTTGGACTATTAATAATGT+AGG	+	chr1_1:80729098-80729117	Msa0047650:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	80726914	80729864	80726914	ID=Msa0047650;Name=Msa0047650
chr1_1	mRNA	80726914	80729864	80726914	ID=Msa0047650-mRNA-1;Parent=Msa0047650;Name=Msa0047650-mRNA-1;_AED=0.58;_eAED=0.58;_QI=59\|0.5\|0.66\|0.66\|1\|1\|3\|0\|151
chr1_1	exon	80726914	80727118	80726914	ID=Msa0047650-mRNA-1:exon:27883;Parent=Msa0047650-mRNA-1
chr1_1	exon	80727207	80727328	80727207	ID=Msa0047650-mRNA-1:exon:27884;Parent=Msa0047650-mRNA-1
chr1_1	exon	80729677	80729864	80729677	ID=Msa0047650-mRNA-1:exon:27885;Parent=Msa0047650-mRNA-1
chr1_1	five_prime_UTR	80726914	80726972	80726914	ID=Msa0047650-mRNA-1:five_prime_utr;Parent=Msa0047650-mRNA-1
chr1_1	CDS	80726973	80727118	80726973	ID=Msa0047650-mRNA-1:cds;Parent=Msa0047650-mRNA-1
chr1_1	CDS	80727207	80727328	80727207	ID=Msa0047650-mRNA-1:cds;Parent=Msa0047650-mRNA-1
chr1_1	CDS	80729677	80729864	80729677	ID=Msa0047650-mRNA-1:cds;Parent=Msa0047650-mRNA-1

Gene Sequence

>Msa0047650

ATGGCTTCCACCGCCGCCAGATCAATGTTCCGATCATCTTCTGCAACCAGATCAGCCTTCCGTGTCGCCTCGGAAGCTAAACCAGCTCGTTCTCCGTTCCGAATGGCATCCAATAAACCTCTCTCGCCATCAACATCCATGTTCAGGTCACCTGTTGAATTGAGTTTTTGTGTCGAATCGATGCTTCCTTACCACACTGTTACTGCTTCTGCGCTCATGACTTCAATGCTTTCCCTTTCAAGCCGCAACTACGCTTGGCTTTCAGAAGCTAAGGATTTTCTGGTTTTCTTGTTTGCTGAAGACGATGAAGAACCCACCAGAAAATCTATTTGGGACTTGAACAACAAATACGAGAAAATAAATCTTGAAGTGAAGATCTTAAACTATCATGCATTAGATATTACGAAAGACATTCAGATTGGATTAGAAGCAAAAAGCTCAATATTAGAAATTTGA

Protein sequence

>Msa0047650

MASTAARSMFRSSSATRSAFRVASEAKPARSPFRMASNKPLSPSTSMFRSPVELSFCVESMLPYHTVTASALMTSMLSLSSRNYAWLSEAKDFLVFLFAEDDEEPTRKSIWDLNNKYEKINLEVKILNYHALDITKDIQIGLEAKSSILEI*