AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0340560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0340560 MtrunA17_Chr3g0121631 91.935 124 8 1 1 124 1 122 2.06e-74 218
Msa0340560 MtrunA17_Chr4g0061451 52.893 121 52 3 1 116 1 121 2.48e-36 125
Msa0340560 MtrunA17_Chr7g0219021 48.031 127 59 3 1 124 1 123 1.03e-34 120
Msa0340560 MtrunA17_Chr3g0086861 43.511 131 63 3 1 124 1 127 2.30e-30 112
Msa0340560 MtrunA17_Chr7g0219031 46.729 107 54 2 1 104 1 107 4.90e-28 99.8
Msa0340560 MtrunA17_Chr3g0119311 50.588 85 41 1 38 121 36 120 2.92e-26 95.5
Msa0340560 MtrunA17_Chr7g0266751 46.535 101 50 2 25 121 19 119 1.20e-24 91.7
Msa0340560 MtrunA17_Chr4g0035001 51.316 76 36 1 39 113 7 82 1.30e-23 89.7
Msa0340560 MtrunA17_Chr4g0035051 48.684 76 38 1 39 113 9 84 8.87e-23 86.3
Msa0340560 MtrunA17_Chr4g0031091 43.820 89 49 1 28 115 23 111 1.60e-22 85.5
Msa0340560 MtrunA17_Chr4g0035031 50.000 76 37 1 39 113 9 84 8.28e-22 85.9
Msa0340560 MtrunA17_Chr2g0278661 50.649 77 37 1 39 114 8 84 9.77e-20 81.6
Msa0340560 MtrunA17_Chr3g0116631 44.318 88 44 2 39 125 207 290 1.73e-19 81.6
Msa0340560 MtrunA17_Chr3g0116631 39.362 94 53 2 31 123 102 192 1.65e-11 59.7
Msa0340560 MtrunA17_Chr7g0263001 41.322 121 61 4 1 117 1 115 5.14e-19 77.4
Msa0340560 MtrunA17_Chr1g0181111 49.367 79 39 1 39 116 197 275 7.07e-19 79.7
Msa0340560 MtrunA17_Chr4g0042161 43.137 102 51 3 33 131 44 141 1.73e-18 75.9
Msa0340560 MtrunA17_Chr1g0198431 36.792 106 63 2 24 128 178 280 1.22e-17 76.6
Msa0340560 MtrunA17_Chr4g0058151 44.304 79 43 1 39 116 217 295 5.15e-17 75.1
Msa0340560 MtrunA17_Chr4g0058151 43.529 85 45 2 39 122 123 205 1.94e-12 62.4
Msa0340560 MtrunA17_Chr3g0101061 45.055 91 46 3 29 116 28 117 6.56e-17 75.9
Msa0340560 MtrunA17_Chr1g0168601 45.679 81 43 1 39 118 154 234 8.50e-17 73.6
Msa0340560 MtrunA17_Chr4g0050781 41.250 80 46 1 39 117 8 87 1.76e-16 72.4
Msa0340560 MtrunA17_Chr6g0449481 33.945 109 63 2 33 132 128 236 1.25e-15 72.0
Msa0340560 MtrunA17_Chr1g0210241 37.647 85 52 1 36 119 5 89 2.00e-15 70.5
Msa0340560 MtrunA17_Chr3g0110211 42.697 89 49 2 38 124 112 200 5.10e-15 70.1
Msa0340560 MtrunA17_Chr1g0201001 34.043 94 61 1 40 132 31 124 9.29e-15 69.7
Msa0340560 MtrunA17_Chr3g0144311 35.714 84 54 0 38 121 29 112 1.07e-14 68.6
Msa0340560 MtrunA17_Chr4g0038111 38.462 104 59 2 32 131 333 435 1.53e-14 68.9
Msa0340560 MtrunA17_Chr1g0186351 36.471 85 54 0 34 118 108 192 1.79e-14 68.6
Msa0340560 MtrunA17_Chr1g0186351 36.709 79 49 1 39 117 7 84 9.82e-12 60.8
Msa0340560 MtrunA17_Chr7g0276531 37.662 77 48 0 38 114 18 94 2.78e-14 67.0
Msa0340560 MtrunA17_Chr3g0088511 35.556 90 54 3 24 110 17 105 2.82e-14 64.7
Msa0340560 MtrunA17_Chr6g0481071 35.000 80 51 1 38 116 95 174 3.18e-14 65.9
Msa0340560 MtrunA17_Chr4g0070231 45.333 75 40 1 40 113 133 207 3.56e-14 67.8
Msa0340560 MtrunA17_Chr4g0017401 35.484 93 56 1 38 126 16 108 3.62e-14 67.0
Msa0340560 MtrunA17_Chr5g0447761 41.250 80 46 1 37 116 5 83 3.92e-14 67.8
Msa0340560 MtrunA17_Chr8g0344491 45.122 82 43 2 40 120 149 229 4.49e-14 67.4
Msa0340560 MtrunA17_Chr8g0346011 42.029 69 40 0 38 106 37 105 5.85e-14 66.6
Msa0340560 MtrunA17_Chr7g0267571 40.230 87 51 1 36 121 210 296 6.06e-14 66.6
Msa0340560 MtrunA17_Chr7g0267571 37.333 75 46 1 39 112 110 184 8.19e-11 58.2
Msa0340560 MtrunA17_Chr4g0042151 32.479 117 73 3 2 116 11 123 2.01e-13 63.9
Msa0340560 MtrunA17_Chr5g0430471 33.673 98 56 2 23 111 16 113 2.34e-13 62.8
Msa0340560 MtrunA17_Chr1g0163591 41.176 85 49 1 37 121 161 244 2.62e-13 65.5
Msa0340560 MtrunA17_Chr2g0290191 40.244 82 48 1 35 116 1 81 2.67e-13 65.5
Msa0340560 MtrunA17_Chr2g0290191 27.869 122 79 2 9 130 85 197 1.48e-11 60.5
Msa0340560 MtrunA17_Chr3g0135111 34.118 85 56 0 33 117 112 196 3.94e-13 64.7
Msa0340560 MtrunA17_Chr3g0135111 38.636 88 53 1 29 116 1 87 7.21e-12 61.2
Msa0340560 MtrunA17_Chr1g0153181 37.000 100 59 2 19 114 10 109 4.20e-13 64.7
Msa0340560 MtrunA17_Chr2g0327921 36.250 80 50 1 38 116 7 86 4.49e-13 60.5
Msa0340560 MtrunA17_Chr3g0127751 40.260 77 44 1 38 112 26 102 4.82e-13 63.9
Msa0340560 MtrunA17_Chr5g0426311 35.065 77 50 0 38 114 16 92 6.99e-13 63.5
Msa0340560 MtrunA17_Chr7g0268551 38.824 85 51 1 38 121 348 432 7.40e-13 63.9
Msa0340560 MtrunA17_Chr4g0065741 37.500 80 49 1 36 114 69 148 1.31e-12 61.2
Msa0340560 MtrunA17_Chr5g0419351 36.250 80 50 1 35 113 7 86 1.69e-12 61.2
Msa0340560 MtrunA17_Chr3g0139111 42.683 82 45 2 40 120 147 227 1.85e-12 62.8
Msa0340560 MtrunA17_Chr8g0360551 43.056 72 41 0 35 106 70 141 1.93e-12 62.8
Msa0340560 MtrunA17_Chr8g0375101 36.145 83 52 1 35 117 1 82 7.07e-12 61.2
Msa0340560 MtrunA17_Chr1g0177101 37.349 83 51 1 36 117 205 287 9.56e-12 60.5
Msa0340560 MtrunA17_Chr1g0153871 36.364 88 54 2 40 125 192 279 9.84e-12 60.8
Msa0340560 MtrunA17_Chr4g0018581 39.024 82 49 1 33 113 446 527 1.55e-11 60.5
Msa0340560 MtrunA17_Chr4g0045921 30.769 91 61 1 35 125 160 248 2.67e-11 59.7
Msa0340560 MtrunA17_Chr4g0045921 39.189 74 45 0 33 106 71 144 5.72e-11 58.5
Msa0340560 MtrunA17_Chr4g0044541 37.975 79 48 1 37 114 33 111 3.97e-11 59.3
Msa0340560 MtrunA17_Chr2g0328791 39.759 83 48 2 40 120 110 192 4.07e-11 58.9
Msa0340560 MtrunA17_Chr5g0402191 36.145 83 52 1 35 117 1 82 7.19e-11 58.5
Msa0340560 MtrunA17_Chr8g0383661 34.615 78 50 1 39 115 304 381 8.67e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0340560 AT3G23830.2 59.350 123 45 4 1 122 1 119 3.73e-44 141
Msa0340560 AT3G23830.1 59.350 123 45 4 1 122 1 119 3.73e-44 141
Msa0340560 AT4G13850.4 62.393 117 40 3 1 116 1 114 5.07e-43 138
Msa0340560 AT4G13850.3 60.976 123 43 4 1 122 1 119 5.57e-43 139
Msa0340560 AT4G13850.2 60.976 123 43 4 1 122 1 119 7.30e-43 139
Msa0340560 AT4G13850.1 60.976 123 43 4 1 122 1 119 8.48e-43 139
Msa0340560 AT5G61030.2 55.462 119 50 2 1 116 1 119 7.59e-40 135
Msa0340560 AT5G61030.1 55.462 119 50 2 1 116 1 119 7.59e-40 135
Msa0340560 AT1G74230.1 53.043 115 50 1 1 115 1 111 4.89e-37 127
Msa0340560 AT5G47320.1 55.172 116 44 3 1 115 1 109 1.16e-33 117
Msa0340560 AT3G08000.1 58.333 84 34 1 39 121 42 125 1.42e-27 99.8
Msa0340560 AT5G06210.1 50.000 96 45 2 33 127 29 122 1.78e-23 89.4
Msa0340560 AT2G21660.2 48.684 76 38 1 39 113 9 84 2.34e-21 84.3
Msa0340560 AT5G59865.1 47.191 89 46 1 36 123 24 112 3.20e-21 82.4
Msa0340560 AT2G21660.1 48.684 76 38 1 39 113 9 84 3.48e-21 84.3
Msa0340560 AT2G37220.1 53.165 79 36 1 39 116 205 283 5.46e-21 85.9
Msa0340560 AT2G37220.1 41.860 86 43 2 39 123 92 171 3.68e-12 62.0
Msa0340560 AT3G53460.1 50.602 83 40 1 39 120 258 340 1.73e-20 85.5
Msa0340560 AT3G53460.1 43.023 86 42 2 39 123 100 179 5.92e-11 58.9
Msa0340560 AT3G53460.3 50.602 83 40 1 39 120 258 340 1.73e-20 85.5
Msa0340560 AT3G53460.3 43.023 86 42 2 39 123 100 179 5.92e-11 58.9
Msa0340560 AT3G53460.2 50.602 83 40 1 39 120 258 340 1.73e-20 85.5
Msa0340560 AT3G53460.2 43.023 86 42 2 39 123 100 179 5.92e-11 58.9
Msa0340560 AT4G39260.3 40.000 95 47 2 39 132 7 92 1.92e-20 80.1
Msa0340560 AT3G53460.4 50.602 83 40 1 39 120 279 361 2.01e-20 85.5
Msa0340560 AT1G18630.1 40.000 115 63 3 1 114 4 113 1.71e-19 79.3
Msa0340560 AT4G24770.1 47.126 87 42 2 39 124 245 328 1.00e-18 80.5
Msa0340560 AT4G24770.1 39.000 100 57 2 25 123 137 233 3.50e-12 62.4
Msa0340560 AT4G24770.2 47.126 87 42 2 39 124 245 328 1.00e-18 80.5
Msa0340560 AT4G24770.2 39.000 100 57 2 25 123 137 233 3.50e-12 62.4
Msa0340560 AT3G26420.1 45.882 85 45 1 39 122 8 92 1.16e-18 79.0
Msa0340560 AT5G50250.1 44.304 79 43 1 39 116 208 286 6.68e-18 77.8
Msa0340560 AT5G50250.1 43.678 87 45 2 38 123 113 196 9.93e-13 63.5
Msa0340560 AT2G37510.1 48.052 77 39 1 35 110 31 107 7.27e-18 74.7
Msa0340560 AT4G39260.2 40.789 76 44 1 39 113 7 82 2.14e-17 73.2
Msa0340560 AT4G39260.1 40.789 76 44 1 39 113 7 82 5.68e-17 73.2
Msa0340560 AT1G60000.1 48.193 83 42 1 36 117 175 257 6.54e-17 74.7
Msa0340560 AT4G13860.1 46.667 75 39 1 37 110 2 76 7.97e-17 70.5
Msa0340560 AT3G46020.1 42.500 80 45 1 37 115 6 85 2.31e-16 69.7
Msa0340560 AT5G54580.1 42.683 82 46 1 37 117 55 136 5.36e-16 70.1
Msa0340560 AT3G20930.1 36.842 95 59 1 35 128 279 373 1.01e-15 72.4
Msa0340560 AT1G76460.1 38.462 91 53 1 38 128 24 111 1.30e-15 71.6
Msa0340560 AT5G55550.5 43.038 79 44 1 39 117 7 84 2.99e-15 71.2
Msa0340560 AT5G55550.4 43.038 79 44 1 39 117 7 84 2.99e-15 71.2
Msa0340560 AT5G55550.9 43.038 79 44 1 39 117 7 84 2.99e-15 71.2
Msa0340560 AT5G55550.7 43.038 79 44 1 39 117 7 84 2.99e-15 71.2
Msa0340560 AT5G55550.1 43.038 79 44 1 39 117 7 84 2.99e-15 71.2
Msa0340560 AT5G55550.2 43.038 79 44 1 39 117 7 84 3.20e-15 71.2
Msa0340560 AT5G55550.3 43.038 79 44 1 39 117 7 84 3.20e-15 71.2
Msa0340560 AT5G55550.6 43.038 79 44 1 39 117 7 84 3.20e-15 71.2
Msa0340560 AT5G55550.11 43.038 79 44 1 39 117 7 84 3.20e-15 71.2
Msa0340560 AT3G52380.1 41.573 89 51 1 36 123 217 305 3.32e-15 70.9
Msa0340560 AT5G55550.8 45.714 70 37 1 39 108 7 75 8.07e-15 70.1
Msa0340560 AT5G55550.10 45.714 70 37 1 39 108 7 75 8.44e-15 70.1
Msa0340560 AT1G60650.2 38.000 100 57 2 37 131 11 110 8.81e-15 69.3
Msa0340560 AT1G60650.1 38.000 100 57 2 37 131 11 110 8.81e-15 69.3
Msa0340560 AT2G22100.1 37.778 90 56 0 33 122 158 247 1.26e-14 69.3
Msa0340560 AT1G54080.1 43.038 79 44 1 36 113 146 224 1.80e-14 68.9
Msa0340560 AT1G22910.1 38.961 77 47 0 38 114 13 89 1.92e-14 67.8
Msa0340560 AT1G22910.2 38.961 77 47 0 38 114 13 89 1.96e-14 67.8
Msa0340560 AT2G46780.1 37.234 94 54 1 38 126 22 115 2.05e-14 68.6
Msa0340560 AT2G46780.2 37.234 94 54 1 38 126 22 115 2.05e-14 68.6
Msa0340560 AT1G33470.2 42.029 69 40 0 38 106 7 75 2.10e-14 67.8
Msa0340560 AT1G33470.1 39.474 76 46 0 38 113 7 82 2.21e-14 67.8
Msa0340560 AT3G07810.1 41.026 78 45 1 39 116 7 83 2.25e-14 68.9
Msa0340560 AT3G07810.2 41.026 78 45 1 39 116 7 83 2.30e-14 68.9
Msa0340560 AT1G33470.3 39.474 76 46 0 38 113 7 82 2.36e-14 67.4
Msa0340560 AT3G14100.1 44.000 75 41 1 40 113 146 220 2.66e-14 68.6
Msa0340560 AT5G04280.1 39.744 78 46 1 38 114 7 84 2.97e-14 68.2
Msa0340560 AT2G18510.1 41.573 89 50 2 38 124 112 200 2.97e-14 68.2
Msa0340560 AT1G48920.1 39.000 100 56 1 37 131 296 395 3.30e-14 68.6
Msa0340560 AT4G26650.1 42.308 78 44 1 39 116 16 92 3.51e-14 68.2
Msa0340560 AT4G26650.1 31.034 87 60 0 37 123 121 207 2.81e-11 60.1
Msa0340560 AT4G26650.2 42.308 78 44 1 39 116 13 89 3.68e-14 68.2
Msa0340560 AT4G26650.2 31.034 87 60 0 37 123 118 204 2.97e-11 60.1
Msa0340560 AT1G20880.2 38.961 77 47 0 38 114 24 100 4.08e-14 67.4
Msa0340560 AT1G20880.3 38.961 77 47 0 38 114 24 100 4.08e-14 67.4
Msa0340560 AT1G20880.1 38.961 77 47 0 38 114 24 100 4.08e-14 67.4
Msa0340560 AT1G22910.3 38.961 77 47 0 38 114 13 89 5.19e-14 67.4
Msa0340560 AT1G22330.1 36.559 93 56 1 38 130 17 106 5.91e-14 67.0
Msa0340560 AT4G13860.2 53.448 58 26 1 54 110 2 59 6.82e-14 62.4
Msa0340560 AT5G47620.1 39.759 83 49 1 34 116 2 83 7.42e-14 67.4
Msa0340560 AT5G47620.1 33.333 78 52 0 36 113 104 181 3.12e-11 59.7
Msa0340560 AT5G47620.2 39.759 83 49 1 34 116 2 83 7.42e-14 67.4
Msa0340560 AT5G47620.2 33.333 78 52 0 36 113 104 181 3.12e-11 59.7
Msa0340560 AT5G47620.5 39.759 83 49 1 34 116 2 83 7.42e-14 67.4
Msa0340560 AT5G47620.5 33.333 78 52 0 36 113 104 181 3.12e-11 59.7
Msa0340560 AT1G78260.1 36.364 77 49 0 38 114 17 93 9.50e-14 66.2
Msa0340560 AT5G47620.4 53.704 54 25 0 34 87 2 55 1.38e-13 66.6
Msa0340560 AT5G47620.4 33.333 78 52 0 36 113 126 203 3.28e-11 59.7
Msa0340560 AT4G39260.4 38.983 59 36 0 39 97 7 65 1.53e-13 62.8
Msa0340560 AT1G78260.2 36.364 77 49 0 38 114 17 93 1.57e-13 65.9
Msa0340560 AT3G13224.3 36.667 90 56 1 30 118 92 181 2.49e-13 65.5
Msa0340560 AT3G13224.2 36.667 90 56 1 30 118 101 190 2.96e-13 65.5
Msa0340560 AT3G54770.3 36.364 77 49 0 38 114 17 93 3.24e-13 64.7
Msa0340560 AT2G27330.1 31.579 95 64 1 37 130 20 114 3.39e-13 62.0
Msa0340560 AT3G13224.1 45.588 68 37 0 30 97 101 168 3.42e-13 64.3
Msa0340560 AT3G54770.1 36.364 77 49 0 38 114 17 93 3.54e-13 64.7
Msa0340560 AT1G17640.2 34.694 98 61 2 36 131 155 251 4.28e-13 65.1
Msa0340560 AT1G17640.1 34.694 98 61 2 36 131 155 251 4.28e-13 65.1
Msa0340560 AT1G17640.3 34.694 98 61 2 36 131 155 251 4.28e-13 65.1
Msa0340560 AT2G21690.1 39.437 71 42 1 41 110 10 80 5.86e-13 61.2
Msa0340560 AT1G17370.2 44.000 75 41 1 40 113 141 215 9.69e-13 63.9
Msa0340560 AT1G71800.1 35.443 79 49 2 40 116 11 89 1.06e-12 63.9
Msa0340560 AT1G17370.1 44.000 75 41 1 40 113 141 215 1.06e-12 63.9
Msa0340560 AT4G14300.1 40.506 79 46 1 39 117 7 84 1.25e-12 63.9
Msa0340560 AT4G14300.1 34.146 82 54 0 37 118 109 190 4.56e-12 62.0
Msa0340560 AT4G14300.2 40.506 79 46 1 39 117 7 84 1.25e-12 63.9
Msa0340560 AT4G14300.2 34.146 82 54 0 37 118 109 190 4.56e-12 62.0
Msa0340560 AT1G49760.1 43.590 78 43 1 37 113 44 121 1.36e-12 63.9
Msa0340560 AT1G49760.2 43.590 78 43 1 37 113 44 121 1.36e-12 63.9
Msa0340560 AT1G01080.1 37.975 79 49 0 32 110 206 284 1.43e-12 63.2
Msa0340560 AT2G33410.1 31.579 114 72 1 11 118 77 190 1.45e-12 63.5
Msa0340560 AT2G33410.1 37.349 83 51 1 39 121 7 88 2.08e-12 63.2
Msa0340560 AT1G01080.2 37.975 79 49 0 32 110 207 285 1.59e-12 63.2
Msa0340560 AT1G54080.2 40.964 83 44 2 36 113 146 228 1.93e-12 63.2
Msa0340560 AT4G00830.2 34.783 92 57 2 26 116 106 195 2.29e-12 63.2
Msa0340560 AT4G00830.1 34.783 92 57 2 26 116 106 195 2.29e-12 63.2
Msa0340560 AT4G00830.6 34.783 92 57 2 26 116 106 195 2.29e-12 63.2
Msa0340560 AT4G00830.3 34.783 92 57 2 26 116 106 195 2.30e-12 63.2
Msa0340560 AT4G00830.5 34.783 92 57 2 26 116 106 195 2.30e-12 63.2
Msa0340560 AT2G37510.2 36.275 102 39 2 35 110 31 132 2.91e-12 60.8
Msa0340560 AT2G19380.1 33.333 84 56 0 33 116 403 486 2.95e-12 62.8
Msa0340560 AT5G11412.1 34.211 76 50 0 38 113 52 127 3.20e-12 60.8
Msa0340560 AT5G53720.1 36.364 77 49 0 37 113 8 84 3.91e-12 58.9
Msa0340560 AT4G03110.1 36.471 85 53 1 38 121 349 433 4.31e-12 62.4
Msa0340560 AT1G73530.1 33.333 81 53 1 37 116 76 156 4.77e-12 60.5
Msa0340560 AT4G20030.1 34.286 105 60 4 26 125 31 131 5.74e-12 59.7
Msa0340560 AT3G18610.1 39.535 86 49 2 37 122 383 465 6.25e-12 62.0
Msa0340560 AT5G53680.1 34.615 78 49 1 38 113 13 90 8.99e-12 59.3
Msa0340560 AT3G18610.3 39.535 86 49 2 37 122 353 435 9.69e-12 61.2
Msa0340560 AT1G03457.3 34.177 79 51 1 38 115 225 303 1.02e-11 60.8
Msa0340560 AT1G47490.2 37.234 94 57 2 40 131 199 292 1.25e-11 60.8
Msa0340560 AT3G18610.2 39.535 86 49 2 37 122 323 405 1.31e-11 60.8
Msa0340560 AT5G51300.1 36.585 82 51 1 33 113 475 556 1.71e-11 60.8
Msa0340560 AT5G51300.2 36.585 82 51 1 33 113 475 556 1.71e-11 60.8
Msa0340560 AT5G51300.3 36.585 82 51 1 33 113 475 556 1.71e-11 60.8
Msa0340560 AT1G47490.1 37.234 94 57 2 40 131 199 292 2.16e-11 60.5
Msa0340560 AT5G47620.3 33.766 77 51 0 37 113 45 121 2.25e-11 60.1
Msa0340560 AT3G15010.1 40.278 72 43 0 35 106 72 143 3.18e-11 59.7
Msa0340560 AT3G15010.2 40.278 72 43 0 35 106 72 143 3.18e-11 59.7
Msa0340560 AT5G54900.1 39.506 81 47 2 40 118 156 236 3.32e-11 59.7
Msa0340560 AT5G40490.2 32.927 82 54 1 37 117 112 193 3.44e-11 59.7
Msa0340560 AT1G13690.1 37.333 75 46 1 40 113 15 89 3.70e-11 58.2
Msa0340560 AT3G10400.1 37.975 79 48 1 38 115 57 135 4.90e-11 58.9
Msa0340560 AT1G47500.2 36.170 94 58 2 40 131 201 294 7.92e-11 58.5
Msa0340560 AT1G47500.1 36.170 94 58 2 40 131 201 294 9.05e-11 58.5

Find 45 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGGACAGGATTTAAA+TGG 0.332884 2_4:+61912210 Msa0340560:CDS
ACTGGAAGATCAAGGGGATT+TGG 0.334462 2_4:+61912141 Msa0340560:CDS
ATGGGTTTCTGTAACAAGAT+TGG 0.366720 2_4:+61911919 Msa0340560:CDS
TGCAACTTCAGCACTCTCTA+TGG 0.384662 2_4:+61912191 Msa0340560:CDS
GTGGCTATGGCGGTGGTGGT+TGG 0.397607 2_4:+61912292 Msa0340560:CDS
CATAGGACACACGAATGTTT+CGG 0.398236 2_4:-61912233 None:intergenic
GAGGAGGAGCTTGAGTGCTC+TGG 0.408995 2_4:-61911966 None:intergenic
TGTTGATGATCAGTCTCTTA+AGG 0.437494 2_4:+61912062 Msa0340560:CDS
ACCACCGCCATAGCCACCTC+CGG 0.442288 2_4:-61912286 None:intergenic
CCTCGTCCCGGAGGTGGCTA+TGG 0.454161 2_4:+61912279 Msa0340560:CDS
TAGTTAAGCATGGATGAAAC+AGG 0.461015 2_4:-61911988 None:intergenic
GGAGGTGGCTATGGCGGTGG+TGG 0.469031 2_4:+61912288 Msa0340560:CDS
AGCACTCTCTATGGATGGAC+AGG 0.475981 2_4:+61912200 Msa0340560:CDS
GGTGGACCTCGTCCCGGAGG+TGG 0.479282 2_4:+61912273 Msa0340560:CDS
CCCGGAGGTGGCTATGGCGG+TGG 0.480523 2_4:+61912285 Msa0340560:CDS
GATCATCAACATGGTAAGAA+AGG 0.505312 2_4:-61912050 None:intergenic
ATAGAGACACTGGAAGATCA+AGG 0.517492 2_4:+61912133 Msa0340560:CDS
AGGAGGAGCTTGAGTGCTCT+GGG 0.530346 2_4:-61911965 None:intergenic
CAGGCTGGTGGACCTCGTCC+CGG 0.532193 2_4:+61912267 Msa0340560:CDS
AAGGATGCATTTACAACCTA+CGG 0.532917 2_4:+61912081 Msa0340560:CDS
CCATAGCCACCTCCGGGACG+AGG 0.541011 2_4:-61912279 None:intergenic
GGGTACCACCTTGCCTCAAG+AGG 0.543148 2_4:-61911945 None:intergenic
GTCCTATGCAAATGATAGAC+AGG 0.554294 2_4:+61912248 Msa0340560:CDS
CCACCGCCATAGCCACCTCC+GGG 0.578140 2_4:-61912285 None:intergenic
GTTATCACTGATAGAGACAC+TGG 0.581409 2_4:+61912123 Msa0340560:CDS
ACTTCAGCACTCTCTATGGA+TGG 0.581897 2_4:+61912195 Msa0340560:CDS
TATGCAAATGATAGACAGGC+TGG 0.581925 2_4:+61912252 Msa0340560:CDS
TGCCTCAACCACTTCTCCGT+AGG 0.589946 2_4:-61912097 None:intergenic
TAGAGACACTGGAAGATCAA+GGG 0.594765 2_4:+61912134 Msa0340560:CDS
CGTCCCGGAGGTGGCTATGG+CGG 0.598189 2_4:+61912282 Msa0340560:CDS
AGAGACACTGGAAGATCAAG+GGG 0.604078 2_4:+61912135 Msa0340560:CDS
ATTTACAACCTACGGAGAAG+TGG 0.607456 2_4:+61912089 Msa0340560:CDS
AGCATGGATGAAACAGGAGG+AGG 0.619418 2_4:-61911982 None:intergenic
AACCTACGGAGAAGTGGTTG+AGG 0.621999 2_4:+61912095 Msa0340560:CDS
GTGGCGCAAGTAGTTAAGCA+TGG 0.622132 2_4:-61911998 None:intergenic
ATTGGAAACCTCTTGAGGCA+AGG 0.631105 2_4:+61911937 Msa0340560:CDS
TGAAGTTGCAGACTCTTGAG+TGG 0.654691 2_4:-61912178 None:intergenic
AGCCTGTCTATCATTTGCAT+AGG 0.655393 2_4:-61912250 None:intergenic
ACAAGATTGGAAACCTCTTG+AGG 0.658250 2_4:+61911932 Msa0340560:CDS
GCAAATGATAGACAGGCTGG+TGG 0.673979 2_4:+61912255 Msa0340560:CDS
GCTGGTGGACCTCGTCCCGG+AGG 0.683775 2_4:+61912270 Msa0340560:CDS
GGAAACCTCTTGAGGCAAGG+TGG 0.688707 2_4:+61911940 Msa0340560:CDS
TAAGAGACTGATCATCAACA+TGG 0.702631 2_4:-61912059 None:intergenic
TTAAGCATGGATGAAACAGG+AGG 0.710474 2_4:-61911985 None:intergenic
AAAGCTTGCTTGAAGACATG+TGG 0.718559 2_4:-61912017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGGGTTTCTGTAACAAGAT+TGG + chr2_4:61911919-61911938 Msa0340560:CDS 35.0%
TAGTTAAGCATGGATGAAAC+AGG - chr2_4:61911991-61912010 None:intergenic 35.0%
! TCTTCAAGCAAGCTTTTCAT+TGG + chr2_4:61912024-61912043 Msa0340560:CDS 35.0%
GATCATCAACATGGTAAGAA+AGG - chr2_4:61912053-61912072 None:intergenic 35.0%
TAAGAGACTGATCATCAACA+TGG - chr2_4:61912062-61912081 None:intergenic 35.0%
!! TGTTGATGATCAGTCTCTTA+AGG + chr2_4:61912062-61912081 Msa0340560:CDS 35.0%
AAGGATGCATTTACAACCTA+CGG + chr2_4:61912081-61912100 Msa0340560:CDS 35.0%
ATGGATGGACAGGATTTAAA+TGG + chr2_4:61912210-61912229 Msa0340560:CDS 35.0%
ACAAGATTGGAAACCTCTTG+AGG + chr2_4:61911932-61911951 Msa0340560:CDS 40.0%
TTAAGCATGGATGAAACAGG+AGG - chr2_4:61911988-61912007 None:intergenic 40.0%
! AAAGCTTGCTTGAAGACATG+TGG - chr2_4:61912020-61912039 None:intergenic 40.0%
! TCAAGCAAGCTTTTCATTGG+AGG + chr2_4:61912027-61912046 Msa0340560:CDS 40.0%
ATTTACAACCTACGGAGAAG+TGG + chr2_4:61912089-61912108 Msa0340560:CDS 40.0%
GTTATCACTGATAGAGACAC+TGG + chr2_4:61912123-61912142 Msa0340560:CDS 40.0%
ATAGAGACACTGGAAGATCA+AGG + chr2_4:61912133-61912152 Msa0340560:CDS 40.0%
TAGAGACACTGGAAGATCAA+GGG + chr2_4:61912134-61912153 Msa0340560:CDS 40.0%
CATAGGACACACGAATGTTT+CGG - chr2_4:61912236-61912255 None:intergenic 40.0%
GTCCTATGCAAATGATAGAC+AGG + chr2_4:61912248-61912267 Msa0340560:CDS 40.0%
AGCCTGTCTATCATTTGCAT+AGG - chr2_4:61912253-61912272 None:intergenic 40.0%
TATGCAAATGATAGACAGGC+TGG + chr2_4:61912252-61912271 Msa0340560:CDS 40.0%
ATTGGAAACCTCTTGAGGCA+AGG + chr2_4:61911937-61911956 Msa0340560:CDS 45.0%
AGAGACACTGGAAGATCAAG+GGG + chr2_4:61912135-61912154 Msa0340560:CDS 45.0%
ACTGGAAGATCAAGGGGATT+TGG + chr2_4:61912141-61912160 Msa0340560:CDS 45.0%
TGAAGTTGCAGACTCTTGAG+TGG - chr2_4:61912181-61912200 None:intergenic 45.0%
TGCAACTTCAGCACTCTCTA+TGG + chr2_4:61912191-61912210 Msa0340560:CDS 45.0%
ACTTCAGCACTCTCTATGGA+TGG + chr2_4:61912195-61912214 Msa0340560:CDS 45.0%
AGCATGGATGAAACAGGAGG+AGG - chr2_4:61911985-61912004 None:intergenic 50.0%
GTGGCGCAAGTAGTTAAGCA+TGG - chr2_4:61912001-61912020 None:intergenic 50.0%
AACCTACGGAGAAGTGGTTG+AGG + chr2_4:61912095-61912114 Msa0340560:CDS 50.0%
AGCACTCTCTATGGATGGAC+AGG + chr2_4:61912200-61912219 Msa0340560:CDS 50.0%
GCAAATGATAGACAGGCTGG+TGG + chr2_4:61912255-61912274 Msa0340560:CDS 50.0%
GGAAACCTCTTGAGGCAAGG+TGG + chr2_4:61911940-61911959 Msa0340560:CDS 55.0%
AGGAGGAGCTTGAGTGCTCT+GGG - chr2_4:61911968-61911987 None:intergenic 55.0%
TGCCTCAACCACTTCTCCGT+AGG - chr2_4:61912100-61912119 None:intergenic 55.0%
GGGTACCACCTTGCCTCAAG+AGG - chr2_4:61911948-61911967 None:intergenic 60.0%
GAGGAGGAGCTTGAGTGCTC+TGG - chr2_4:61911969-61911988 None:intergenic 60.0%
ACCACCGCCATAGCCACCTC+CGG - chr2_4:61912289-61912308 None:intergenic 65.0%
! GTGGCTATGGCGGTGGTGGT+TGG + chr2_4:61912292-61912311 Msa0340560:CDS 65.0%
! CAGGCTGGTGGACCTCGTCC+CGG + chr2_4:61912267-61912286 Msa0340560:CDS 70.0%
CCATAGCCACCTCCGGGACG+AGG - chr2_4:61912282-61912301 None:intergenic 70.0%
CCTCGTCCCGGAGGTGGCTA+TGG + chr2_4:61912279-61912298 Msa0340560:CDS 70.0%
CGTCCCGGAGGTGGCTATGG+CGG + chr2_4:61912282-61912301 Msa0340560:CDS 70.0%
CCACCGCCATAGCCACCTCC+GGG - chr2_4:61912288-61912307 None:intergenic 70.0%
GGAGGTGGCTATGGCGGTGG+TGG + chr2_4:61912288-61912307 Msa0340560:CDS 70.0%
! GCTGGTGGACCTCGTCCCGG+AGG + chr2_4:61912270-61912289 Msa0340560:CDS 75.0%
GGTGGACCTCGTCCCGGAGG+TGG + chr2_4:61912273-61912292 Msa0340560:CDS 75.0%
CCCGGAGGTGGCTATGGCGG+TGG + chr2_4:61912285-61912304 Msa0340560:CDS 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 61911919 61912317 61911919 ID=Msa0340560;Name=Msa0340560
chr2_4 mRNA 61911919 61912317 61911919 ID=Msa0340560-mRNA-1;Parent=Msa0340560;Name=Msa0340560-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|132
chr2_4 exon 61911919 61912317 61911919 ID=Msa0340560-mRNA-1:exon:19612;Parent=Msa0340560-mRNA-1
chr2_4 CDS 61911919 61912317 61911919 ID=Msa0340560-mRNA-1:cds;Parent=Msa0340560-mRNA-1
Gene Sequence

>Msa0340560

ATGGGTTTCTGTAACAAGATTGGAAACCTCTTGAGGCAAGGTGGTACCCAGAGCACTCAAGCTCCTCCTCCTGTTTCATCCATGCTTAACTACTTGCGCCACATGTCTTCAAGCAAGCTTTTCATTGGAGGCCTTTCTTACCATGTTGATGATCAGTCTCTTAAGGATGCATTTACAACCTACGGAGAAGTGGTTGAGGCAAGAGTTATCACTGATAGAGACACTGGAAGATCAAGGGGATTTGGATTTGTCAACTTCACCACTCAAGAGTCTGCAACTTCAGCACTCTCTATGGATGGACAGGATTTAAATGGCCGAAACATTCGTGTGTCCTATGCAAATGATAGACAGGCTGGTGGACCTCGTCCCGGAGGTGGCTATGGCGGTGGTGGTTGGTGA
Protein sequence

>Msa0340560

MGFCNKIGNLLRQGGTQSTQAPPPVSSMLNYLRHMSSSKLFIGGLSYHVDDQSLKDAFTTYGEVVEARVITDRDTGRSRGFGFVNFTTQESATSALSMDGQDLNGRNIRVSYANDRQAGGPRPGGGYGGGGW*