AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0077230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077230 MtrunA17_Chr1g0186641 94.286 210 12 0 1 210 1 210 9.68e-143 402
Msa0077230 MtrunA17_Chr1g0186611 83.412 211 32 1 1 208 1 211 2.92e-125 358
Msa0077230 MtrunA17_Chr1g0186601 77.465 213 45 1 1 210 1 213 1.64e-117 338
Msa0077230 MtrunA17_Chr1g0186571 77.619 210 47 0 1 210 1 210 2.92e-115 332
Msa0077230 MtrunA17_Chr1g0186561 75.472 212 48 2 1 208 1 212 8.92e-114 328
Msa0077230 MtrunA17_Chr1g0171301 71.362 213 56 3 1 208 1 213 4.71e-103 301
Msa0077230 MtrunA17_Chr1g0184221 47.005 217 99 2 2 204 5 219 2.11e-66 208
Msa0077230 MtrunA17_Chr7g0255561 40.376 213 119 2 3 208 4 215 3.21e-46 157
Msa0077230 MtrunA17_Chr7g0255581 40.376 213 119 2 3 208 4 215 3.21e-46 157
Msa0077230 MtrunA17_Chr3g0081421 40.594 202 114 2 9 204 13 214 4.25e-45 154
Msa0077230 MtrunA17_Chr3g0081511 40.404 198 115 1 10 204 14 211 3.91e-44 151
Msa0077230 MtrunA17_Chr8g0354121 40.415 193 107 2 10 194 10 202 2.89e-43 149
Msa0077230 MtrunA17_Chr1g0177221 35.577 208 131 2 3 208 8 214 3.86e-42 143
Msa0077230 MtrunA17_Chr1g0160171 37.500 208 128 1 3 208 6 213 1.88e-41 144
Msa0077230 MtrunA17_Chr7g0263101 36.842 209 127 2 2 208 5 210 1.92e-41 144
Msa0077230 MtrunA17_Chr3g0114691 38.235 204 122 1 9 208 16 219 3.28e-41 144
Msa0077230 MtrunA17_Chr3g0114711 36.364 209 126 3 1 204 1 207 1.87e-40 142
Msa0077230 MtrunA17_Chr1g0182291 37.000 200 126 0 9 208 11 210 5.15e-40 140
Msa0077230 MtrunA17_Chr1g0160181 35.238 210 132 1 3 208 8 217 1.40e-39 139
Msa0077230 MtrunA17_Chr3g0114731 34.000 200 132 0 11 210 16 215 4.35e-35 127
Msa0077230 MtrunA17_Chr3g0142131 34.286 210 123 1 10 204 17 226 9.25e-35 127
Msa0077230 MtrunA17_Chr3g0114701 33.824 204 131 2 9 208 5 208 1.05e-34 126
Msa0077230 MtrunA17_Chr6g0463971 39.474 152 91 1 9 160 12 162 4.07e-33 122
Msa0077230 MtrunA17_Chr1g0155761 36.585 164 101 1 10 173 18 178 6.09e-33 122
Msa0077230 MtrunA17_Chr3g0129601 33.173 208 128 4 9 208 25 229 3.40e-32 119
Msa0077230 MtrunA17_Chr3g0129571 35.979 189 113 3 9 189 3 191 1.14e-30 115
Msa0077230 MtrunA17_Chr2g0332001 34.500 200 124 3 9 204 34 230 1.44e-28 110
Msa0077230 MtrunA17_Chr2g0278541 32.394 213 132 3 3 204 2 213 1.91e-28 110
Msa0077230 MtrunA17_Chr2g0278561 35.849 159 102 0 1 159 3 161 2.07e-28 110
Msa0077230 MtrunA17_Chr3g0129591 33.333 204 128 3 9 204 1 204 2.18e-28 109
Msa0077230 MtrunA17_Chr6g0483401 33.702 181 100 2 29 196 26 199 3.31e-28 108
Msa0077230 MtrunA17_Chr8g0334761 35.025 197 125 2 12 205 9 205 4.40e-28 108
Msa0077230 MtrunA17_Chr4g0073241 33.333 207 121 4 9 205 4 203 1.36e-26 104
Msa0077230 MtrunA17_Chr8g0383731 32.804 189 119 1 9 189 1 189 1.53e-26 104
Msa0077230 MtrunA17_Chr3g0129581 33.333 204 128 2 9 204 1 204 1.60e-26 104
Msa0077230 MtrunA17_Chr3g0126101 30.890 191 131 1 14 204 7 196 2.18e-26 103
Msa0077230 MtrunA17_Chr4g0050941 34.637 179 115 2 3 180 2 179 4.03e-26 103
Msa0077230 MtrunA17_Chr5g0408031 35.404 161 104 0 10 170 7 167 4.91e-26 103
Msa0077230 MtrunA17_Chr2g0290611 35.429 175 108 3 9 180 8 180 1.79e-25 101
Msa0077230 MtrunA17_Chr7g0271561 32.886 149 100 0 14 162 8 156 3.47e-25 101
Msa0077230 MtrunA17_Chr4g0050891 33.962 159 105 0 1 159 1 159 3.67e-25 100
Msa0077230 MtrunA17_Chr5g0407991 37.584 149 93 0 12 160 8 156 4.31e-25 100
Msa0077230 MtrunA17_Chr5g0408041 35.443 158 102 0 13 170 11 168 8.86e-25 100
Msa0077230 MtrunA17_Chr2g0278551 34.756 164 106 1 3 166 2 164 1.04e-24 100
Msa0077230 MtrunA17_Chr8g0334711 34.673 199 126 3 12 207 9 206 1.21e-24 96.3
Msa0077230 MtrunA17_Chr2g0284261 30.968 155 107 0 10 164 5 159 2.26e-24 98.6
Msa0077230 MtrunA17_Chr5g0408001 34.783 161 105 0 10 170 7 167 4.17e-24 98.2
Msa0077230 MtrunA17_Chr1g0164831 30.000 200 127 4 12 204 2 195 4.21e-24 97.4
Msa0077230 MtrunA17_Chr6g0451171 34.804 204 124 7 11 210 10 208 8.06e-24 97.1
Msa0077230 MtrunA17_Chr5g0413961 30.808 198 123 3 14 205 15 204 8.70e-24 97.1
Msa0077230 MtrunA17_Chr4g0059311 29.189 185 119 2 27 204 26 205 9.74e-24 96.7
Msa0077230 MtrunA17_Chr5g0407971 32.432 185 119 2 12 191 10 193 7.03e-23 94.7
Msa0077230 MtrunA17_Chr4g0051021 33.333 201 128 3 9 204 10 209 1.04e-22 94.4
Msa0077230 MtrunA17_Chr2g0310441 32.338 201 125 3 12 205 9 205 4.86e-22 92.4
Msa0077230 MtrunA17_Chr6g0451261 33.714 175 104 5 38 205 36 205 5.06e-22 93.6
Msa0077230 MtrunA17_Chr6g0451201 33.149 181 112 7 33 210 32 206 1.17e-21 91.3
Msa0077230 MtrunA17_Chr8g0334771 32.995 197 125 3 14 205 11 205 2.12e-21 90.5
Msa0077230 MtrunA17_Chr8g0334751 36.184 152 96 1 14 164 12 163 3.89e-21 89.7
Msa0077230 MtrunA17_Chr1g0160111 30.882 136 94 0 22 157 16 151 6.21e-21 89.4
Msa0077230 MtrunA17_Chr5g0413921 30.508 177 119 4 33 206 27 202 7.59e-21 88.6
Msa0077230 MtrunA17_Chr5g0413941 28.333 180 119 5 33 206 27 202 8.14e-21 88.6
Msa0077230 MtrunA17_Chr1g0211801 29.121 182 120 4 13 189 11 188 8.56e-21 87.4
Msa0077230 MtrunA17_Chr1g0150151 33.659 205 121 7 10 204 8 207 2.30e-20 87.8
Msa0077230 MtrunA17_Chr4g0051001 33.929 168 108 1 6 173 24 188 3.40e-20 86.7
Msa0077230 MtrunA17_Chr4g0050881 31.343 201 130 3 10 204 6 204 4.91e-20 86.7
Msa0077230 MtrunA17_Chr6g0451251 32.787 183 113 7 33 210 32 209 1.20e-19 85.5
Msa0077230 MtrunA17_Chr8g0369841 33.113 151 101 0 12 162 8 158 2.05e-19 85.1
Msa0077230 MtrunA17_Chr3g0126091 26.606 218 147 5 1 210 1 213 2.14e-19 85.1
Msa0077230 MtrunA17_Chr6g0451211 33.520 179 109 6 33 205 32 206 2.96e-18 81.6
Msa0077230 MtrunA17_Chr5g0413931 27.273 198 134 5 17 208 10 203 3.59e-18 81.3
Msa0077230 MtrunA17_Chr7g0227921 32.000 175 116 3 33 205 31 204 4.74e-18 81.3
Msa0077230 MtrunA17_Chr5g0404801 33.333 117 78 0 13 129 7 123 4.85e-18 76.6
Msa0077230 MtrunA17_Chr2g0278031 33.607 122 81 0 4 125 5 126 1.74e-17 75.5
Msa0077230 MtrunA17_Chr5g0428881 25.694 144 107 0 16 159 21 164 3.58e-16 75.9
Msa0077230 MtrunA17_Chr5g0413951 25.000 180 125 5 33 206 27 202 9.55e-16 74.7
Msa0077230 MtrunA17_Chr8g0334731 34.194 155 95 4 62 210 40 193 1.57e-15 72.0
Msa0077230 MtrunA17_Chr5g0441761 26.064 188 136 1 18 205 19 203 5.80e-15 72.4
Msa0077230 MtrunA17_Chr4g0056081 28.249 177 122 3 39 210 40 216 4.30e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077230 AT5G07050.1 44.048 168 94 0 9 176 13 180 7.89e-46 156
Msa0077230 AT4G08300.1 38.164 207 123 2 7 208 4 210 1.61e-41 144
Msa0077230 AT2G37460.1 36.715 207 131 0 2 208 3 209 5.27e-41 143
Msa0077230 AT1G44800.1 37.624 202 124 1 9 208 6 207 1.16e-40 142
Msa0077230 AT2G39510.1 36.139 202 127 2 9 208 3 204 1.97e-40 142
Msa0077230 AT1G21890.2 37.089 213 123 1 9 210 6 218 1.32e-39 139
Msa0077230 AT4G08290.2 35.238 210 132 2 3 208 2 211 1.39e-39 137
Msa0077230 AT1G21890.1 37.089 213 123 1 9 210 6 218 1.44e-39 140
Msa0077230 AT4G08290.1 35.238 210 132 2 3 208 2 211 9.39e-39 137
Msa0077230 AT3G56620.1 44.667 150 83 0 13 162 10 159 1.64e-36 131
Msa0077230 AT3G53210.3 35.354 198 119 3 13 204 9 203 9.28e-35 124
Msa0077230 AT1G75500.1 33.498 203 120 2 15 205 21 220 9.66e-35 127
Msa0077230 AT1G75500.2 33.498 203 120 2 15 205 21 220 9.66e-35 127
Msa0077230 AT3G53210.1 35.354 198 119 3 13 204 9 203 7.65e-34 124
Msa0077230 AT2G40900.1 34.184 196 126 2 13 208 10 202 8.10e-33 122
Msa0077230 AT1G09380.1 36.913 149 94 0 11 159 5 153 4.13e-31 117
Msa0077230 AT5G13670.1 32.367 207 127 3 13 210 7 209 4.15e-31 117
Msa0077230 AT5G64700.2 33.831 201 124 2 13 204 7 207 1.84e-30 113
Msa0077230 AT3G18200.2 40.458 131 78 0 29 159 50 180 3.67e-30 114
Msa0077230 AT5G64700.1 35.450 189 113 2 13 192 7 195 1.17e-29 113
Msa0077230 AT4G30420.2 31.068 206 129 3 9 205 4 205 9.07e-28 108
Msa0077230 AT3G30340.2 31.313 198 129 2 12 204 9 204 1.04e-26 103
Msa0077230 AT3G30340.1 31.313 198 129 2 12 204 9 204 3.20e-26 103
Msa0077230 AT1G43650.2 31.633 196 133 1 9 204 2 196 6.01e-26 101
Msa0077230 AT1G11450.4 32.143 196 131 2 11 204 13 208 6.49e-26 102
Msa0077230 AT4G01430.2 30.049 203 131 3 10 204 5 204 1.14e-25 101
Msa0077230 AT1G11450.3 32.642 193 128 2 14 204 16 208 1.17e-25 102
Msa0077230 AT4G01430.1 30.049 203 131 3 10 204 5 204 2.18e-25 101
Msa0077230 AT1G43650.1 31.633 196 133 1 9 204 2 196 2.42e-25 101
Msa0077230 AT4G30420.1 31.472 197 122 3 18 205 3 195 2.52e-25 101
Msa0077230 AT2G37450.2 34.359 195 107 1 14 208 9 182 3.78e-25 100
Msa0077230 AT4G01450.3 30.412 194 133 1 11 204 7 198 2.47e-24 96.7
Msa0077230 AT4G24980.1 31.034 174 98 2 31 204 4 155 2.49e-24 97.4
Msa0077230 AT4G01450.4 30.412 194 133 1 11 204 7 198 2.56e-24 97.1
Msa0077230 AT4G01450.1 30.412 194 133 1 11 204 7 198 8.24e-24 97.1
Msa0077230 AT4G01450.2 30.412 194 133 1 11 204 7 198 9.24e-24 97.4
Msa0077230 AT1G11450.5 36.250 160 100 2 47 204 5 164 1.74e-23 95.9
Msa0077230 AT5G40230.1 32.512 203 126 4 10 204 17 216 4.44e-23 95.5
Msa0077230 AT1G01070.1 32.484 157 105 1 9 164 11 167 5.16e-23 95.5
Msa0077230 AT1G11460.1 29.469 207 141 3 2 204 4 209 1.89e-22 93.6
Msa0077230 AT5G45370.2 31.351 185 119 2 15 193 21 203 6.13e-22 92.4
Msa0077230 AT5G45370.4 31.351 185 119 2 15 193 21 203 6.13e-22 92.4
Msa0077230 AT1G11450.2 36.129 155 97 2 52 204 3 157 7.42e-22 91.3
Msa0077230 AT5G40240.1 30.846 201 132 3 10 204 16 215 3.98e-21 90.1
Msa0077230 AT5G40240.3 30.846 201 132 3 10 204 16 215 3.98e-21 90.1
Msa0077230 AT4G28040.1 25.980 204 140 3 9 210 3 197 4.13e-21 90.1
Msa0077230 AT4G28040.5 25.980 204 140 3 9 210 3 197 4.13e-21 90.1
Msa0077230 AT4G28040.3 25.980 204 140 3 9 210 3 197 4.13e-21 90.1
Msa0077230 AT4G28040.4 25.980 204 140 3 9 210 3 197 4.13e-21 90.1
Msa0077230 AT4G28040.2 25.980 204 140 3 9 210 3 197 4.13e-21 90.1
Msa0077230 AT5G45370.1 33.108 148 99 0 15 162 21 168 4.22e-21 90.1
Msa0077230 AT5G40240.2 30.846 201 132 3 10 204 30 229 5.43e-21 90.1
Msa0077230 AT4G01440.4 27.072 181 130 1 11 189 7 187 2.11e-20 87.0
Msa0077230 AT4G01440.3 27.072 181 130 1 11 189 7 187 4.68e-20 86.7
Msa0077230 AT5G40210.1 28.646 192 121 3 18 208 17 193 5.31e-20 86.7
Msa0077230 AT4G01440.1 27.072 181 130 1 11 189 7 187 6.61e-20 86.7
Msa0077230 AT1G70260.1 29.412 153 105 1 14 163 12 164 3.05e-19 85.1
Msa0077230 AT3G28050.1 29.327 208 135 7 1 204 1 200 4.77e-18 81.6
Msa0077230 AT1G68170.2 28.708 209 142 3 3 205 2 209 3.40e-17 79.3
Msa0077230 AT3G53210.2 34.959 123 71 3 88 204 27 146 2.04e-16 76.6
Msa0077230 AT3G28100.2 28.856 201 135 4 18 211 18 217 4.23e-16 74.7
Msa0077230 AT3G45870.9 31.757 148 101 0 12 159 10 157 6.78e-16 74.7
Msa0077230 AT3G45870.1 31.757 148 101 0 12 159 10 157 8.64e-16 75.5
Msa0077230 AT3G45870.8 31.333 150 103 0 10 159 8 157 1.23e-15 74.3
Msa0077230 AT1G68170.1 29.381 194 130 3 18 205 7 199 1.59e-15 74.3
Msa0077230 AT4G15540.1 33.548 155 95 4 10 160 13 163 9.25e-15 72.0
Msa0077230 AT4G15540.2 33.548 155 95 4 10 160 13 163 9.25e-15 72.0
Msa0077230 AT3G28100.3 28.125 192 132 3 18 204 18 208 1.97e-14 70.5
Msa0077230 AT1G01070.2 37.383 107 66 1 59 164 14 120 2.91e-14 70.5
Msa0077230 AT3G28100.1 28.125 192 132 3 18 204 18 208 3.48e-14 70.5
Msa0077230 AT4G19185.1 31.169 154 105 1 10 162 16 169 3.53e-14 70.9
Msa0077230 AT3G28130.6 25.641 195 135 4 18 205 17 208 1.08e-13 67.8
Msa0077230 AT1G60050.1 25.481 208 141 4 14 208 13 219 6.46e-13 67.0
Msa0077230 AT3G28130.4 25.743 202 140 4 10 204 9 207 7.58e-13 66.2
Msa0077230 AT3G28130.2 25.773 194 134 4 18 204 17 207 1.23e-12 66.2
Msa0077230 AT1G25270.1 29.252 147 104 0 13 159 2 148 5.85e-12 64.3
Msa0077230 AT3G28070.2 29.204 113 76 1 96 204 7 119 1.18e-11 62.8
Msa0077230 AT3G28070.3 29.204 113 76 1 96 204 99 211 1.55e-11 62.8
Msa0077230 AT3G28070.1 28.571 133 90 2 76 204 80 211 1.63e-11 62.8
Msa0077230 AT1G11450.1 34.821 112 71 2 95 204 5 116 3.50e-11 61.2
Msa0077230 AT2G37450.3 35.455 110 71 0 99 208 1 110 5.68e-11 60.8
Msa0077230 AT5G47470.2 29.412 119 83 1 39 156 83 201 8.45e-11 60.8
Msa0077230 AT5G47470.3 29.412 119 83 1 39 156 83 201 8.80e-11 60.8
Msa0077230 AT5G47470.1 29.412 119 83 1 39 156 55 173 8.88e-11 60.8

Find 47 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTGACATTTGCTCTCTT+TGG 0.187148 1_2:+54063331 Msa0077230:CDS
TCCCACAAATTTCTCATTAA+AGG 0.200692 1_2:-54063808 None:intergenic
AGCTGACATTTGCTCTCTTT+GGG 0.254016 1_2:+54063332 Msa0077230:CDS
TATCAATAAGAGTTGGATAA+AGG 0.279602 1_2:+54063864 Msa0077230:CDS
TCATCACATCACTAAACTTA+TGG 0.281992 1_2:+54062895 Msa0077230:exon
ACGTACCGGCACGTTGTTGC+CGG 0.310492 1_2:+54063052 Msa0077230:CDS
ATCAATAAGAGTTGGATAAA+GGG 0.339449 1_2:+54063865 Msa0077230:CDS
TTTGGTTGAATATGGATTAG+AGG 0.351226 1_2:-54063832 None:intergenic
CGTACCGGCACGTTGTTGCC+GGG 0.366736 1_2:+54063053 Msa0077230:CDS
GATAAAGGGTTCACTTCTTA+CGG 0.402983 1_2:+54063879 Msa0077230:CDS
AAGTATATCCAACTTGGGTT+AGG 0.404034 1_2:-54062970 None:intergenic
ACCTTTAATGAGAAATTTGT+GGG 0.440140 1_2:+54063807 Msa0077230:CDS
TTCTATATCGTACATTATGC+AGG 0.442746 1_2:+54063921 Msa0077230:CDS
TCTTTATTGCAGTGGTGCTA+AGG 0.445045 1_2:+54063556 Msa0077230:CDS
GACCTTTAATGAGAAATTTG+TGG 0.454162 1_2:+54063806 Msa0077230:CDS
GTTGTACATTGAAGCAAGAA+AGG 0.457079 1_2:-54063514 None:intergenic
AAGAGAGCAAATGTCAGCTT+TGG 0.457572 1_2:-54063328 None:intergenic
TACATTGAAGCAAGAAAGGT+TGG 0.475448 1_2:-54063510 None:intergenic
CCACTGCAATAAAGAAGGTA+AGG 0.476374 1_2:-54063548 None:intergenic
CCTTACCTTCTTTATTGCAG+TGG 0.478004 1_2:+54063548 Msa0077230:CDS
GGGACCTTGATCTCCTTAGC+TGG 0.480268 1_2:+54063757 Msa0077230:CDS
CTCATGCTCCTAACCCAAGT+TGG 0.485428 1_2:+54062962 Msa0077230:CDS
ACTGAAGCTTCCTTCAATCA+TGG 0.487850 1_2:+54063007 Msa0077230:CDS
ATGTTAAAGTATATCCAACT+TGG 0.491933 1_2:-54062976 None:intergenic
CGTGGCATAGCAAAAGTACT+TGG 0.499856 1_2:+54063736 Msa0077230:CDS
CTCTCCAGAAAGTACGCAAA+AGG 0.505275 1_2:-54063088 None:intergenic
GTGCTTCTATCAATAAGAGT+TGG 0.517945 1_2:+54063857 Msa0077230:CDS
AACACCAGCTAAGGAGATCA+AGG 0.524038 1_2:-54063761 None:intergenic
CTTATGTTTATGTTACGTAC+CGG 0.536136 1_2:+54063038 Msa0077230:CDS
CGGTACGTAACATAAACATA+AGG 0.545419 1_2:-54063037 None:intergenic
GTTAGGAGCATGAGCAGGTG+TGG 0.546783 1_2:-54062953 None:intergenic
AGGTCCTTTGTATAATGTCA+TGG 0.556570 1_2:-54063788 None:intergenic
GTACCGGCACGTTGTTGCCG+GGG 0.561621 1_2:+54063054 Msa0077230:CDS
TAGCACCACTGCAATAAAGA+AGG 0.561751 1_2:-54063553 None:intergenic
TGTTAAAGTATATCCAACTT+GGG 0.563922 1_2:-54062975 None:intergenic
CATGGTCATAACACCAGCTA+AGG 0.566284 1_2:-54063770 None:intergenic
CAACCCCGGCAACAACGTGC+CGG 0.573460 1_2:-54063057 None:intergenic
AGGACTCATTCCATGATTGA+AGG 0.576140 1_2:-54063017 None:intergenic
GCTGACATTTGCTCTCTTTG+GGG 0.581077 1_2:+54063333 Msa0077230:CDS
TGTGGCTTGAAATCTCTAAG+AGG 0.590235 1_2:-54062935 None:intergenic
CTTGGGTTAGGAGCATGAGC+AGG 0.592059 1_2:-54062958 None:intergenic
AGGTTGGAGAAGTATACTGC+AGG 0.609030 1_2:-54063494 None:intergenic
AAAAGGAAACATCACAACCC+CGG 0.621141 1_2:-54063071 None:intergenic
CACTGCAATAAAGAAGGTAA+GGG 0.624002 1_2:-54063547 None:intergenic
GTGGCATAGCAAAAGTACTT+GGG 0.649319 1_2:+54063737 Msa0077230:CDS
ATGACCATGACATTATACAA+AGG 0.691521 1_2:+54063784 Msa0077230:CDS
GTTGATCTTCGAAATCCACG+TGG 0.775716 1_2:+54063718 Msa0077230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAACTAAAAATATATCAA+AGG - chr1_2:54063189-54063208 None:intergenic 10.0%
!!! TTTTTTTTTTAAAAAAAAGC+AGG + chr1_2:54063394-54063413 Msa0077230:intron 10.0%
!!! TAATTTTAAAATATTATTGC+AGG + chr1_2:54063439-54063458 Msa0077230:intron 10.0%
!! TAAAACTAAAAATATATCAA+AGG - chr1_2:54063189-54063208 None:intergenic 10.0%
!!! TTTTTTTTTTAAAAAAAAGC+AGG + chr1_2:54063394-54063413 Msa0077230:intron 10.0%
!!! TAATTTTAAAATATTATTGC+AGG + chr1_2:54063439-54063458 Msa0077230:intron 10.0%
!!! ATACTAGTTAATTTTACGTT+CGG - chr1_2:54063156-54063175 None:intergenic 20.0%
!! ACTAAAAATATATCAAAGGT+CGG - chr1_2:54063185-54063204 None:intergenic 20.0%
!!! TCAATATCTTCTGAAAATTT+GGG - chr1_2:54063663-54063682 None:intergenic 20.0%
!!! TTCAATATCTTCTGAAAATT+TGG - chr1_2:54063664-54063683 None:intergenic 20.0%
!!! ATACTAGTTAATTTTACGTT+CGG - chr1_2:54063156-54063175 None:intergenic 20.0%
!! ACTAAAAATATATCAAAGGT+CGG - chr1_2:54063185-54063204 None:intergenic 20.0%
!!! TCAATATCTTCTGAAAATTT+GGG - chr1_2:54063663-54063682 None:intergenic 20.0%
!!! TTCAATATCTTCTGAAAATT+TGG - chr1_2:54063664-54063683 None:intergenic 20.0%
! TGTTAAAGTATATCCAACTT+GGG - chr1_2:54062978-54062997 None:intergenic 25.0%
! ATGTTAAAGTATATCCAACT+TGG - chr1_2:54062979-54062998 None:intergenic 25.0%
! CTAAAAATATATCAAAGGTC+GGG - chr1_2:54063184-54063203 None:intergenic 25.0%
!! ATTTTTGTTCTTTCAGTGTT+AGG + chr1_2:54063358-54063377 Msa0077230:CDS 25.0%
!! TTTTTGTTCTTTCAGTGTTA+GGG + chr1_2:54063359-54063378 Msa0077230:CDS 25.0%
! ACCTTTAATGAGAAATTTGT+GGG + chr1_2:54063807-54063826 Msa0077230:CDS 25.0%
!!! TTATTGATAGAAGCACTTTT+TGG - chr1_2:54063853-54063872 None:intergenic 25.0%
! TATCAATAAGAGTTGGATAA+AGG + chr1_2:54063864-54063883 Msa0077230:CDS 25.0%
! ATCAATAAGAGTTGGATAAA+GGG + chr1_2:54063865-54063884 Msa0077230:CDS 25.0%
! TGTTAAAGTATATCCAACTT+GGG - chr1_2:54062978-54062997 None:intergenic 25.0%
! ATGTTAAAGTATATCCAACT+TGG - chr1_2:54062979-54062998 None:intergenic 25.0%
! CTAAAAATATATCAAAGGTC+GGG - chr1_2:54063184-54063203 None:intergenic 25.0%
!! ATTTTTGTTCTTTCAGTGTT+AGG + chr1_2:54063358-54063377 Msa0077230:CDS 25.0%
!! TTTTTGTTCTTTCAGTGTTA+GGG + chr1_2:54063359-54063378 Msa0077230:CDS 25.0%
! ACCTTTAATGAGAAATTTGT+GGG + chr1_2:54063807-54063826 Msa0077230:CDS 25.0%
!!! TTATTGATAGAAGCACTTTT+TGG - chr1_2:54063853-54063872 None:intergenic 25.0%
! TATCAATAAGAGTTGGATAA+AGG + chr1_2:54063864-54063883 Msa0077230:CDS 25.0%
! ATCAATAAGAGTTGGATAAA+GGG + chr1_2:54063865-54063884 Msa0077230:CDS 25.0%
TCATCACATCACTAAACTTA+TGG + chr1_2:54062895-54062914 Msa0077230:exon 30.0%
CTTATGTTTATGTTACGTAC+CGG + chr1_2:54063038-54063057 Msa0077230:CDS 30.0%
! TATCTTCTGAAAATTTGGGT+GGG - chr1_2:54063659-54063678 None:intergenic 30.0%
! ATATCTTCTGAAAATTTGGG+TGG - chr1_2:54063660-54063679 None:intergenic 30.0%
ATGACCATGACATTATACAA+AGG + chr1_2:54063784-54063803 Msa0077230:CDS 30.0%
GACCTTTAATGAGAAATTTG+TGG + chr1_2:54063806-54063825 Msa0077230:CDS 30.0%
TCCCACAAATTTCTCATTAA+AGG - chr1_2:54063811-54063830 None:intergenic 30.0%
!! TTTGGTTGAATATGGATTAG+AGG - chr1_2:54063835-54063854 None:intergenic 30.0%
!!! AAGCACTTTTTGGTTGAATA+TGG - chr1_2:54063843-54063862 None:intergenic 30.0%
TTCTATATCGTACATTATGC+AGG + chr1_2:54063921-54063940 Msa0077230:CDS 30.0%
TCATCACATCACTAAACTTA+TGG + chr1_2:54062895-54062914 Msa0077230:exon 30.0%
CTTATGTTTATGTTACGTAC+CGG + chr1_2:54063038-54063057 Msa0077230:CDS 30.0%
! TATCTTCTGAAAATTTGGGT+GGG - chr1_2:54063659-54063678 None:intergenic 30.0%
! ATATCTTCTGAAAATTTGGG+TGG - chr1_2:54063660-54063679 None:intergenic 30.0%
ATGACCATGACATTATACAA+AGG + chr1_2:54063784-54063803 Msa0077230:CDS 30.0%
GACCTTTAATGAGAAATTTG+TGG + chr1_2:54063806-54063825 Msa0077230:CDS 30.0%
TCCCACAAATTTCTCATTAA+AGG - chr1_2:54063811-54063830 None:intergenic 30.0%
!! TTTGGTTGAATATGGATTAG+AGG - chr1_2:54063835-54063854 None:intergenic 30.0%
!!! AAGCACTTTTTGGTTGAATA+TGG - chr1_2:54063843-54063862 None:intergenic 30.0%
TTCTATATCGTACATTATGC+AGG + chr1_2:54063921-54063940 Msa0077230:CDS 30.0%
AAGTATATCCAACTTGGGTT+AGG - chr1_2:54062973-54062992 None:intergenic 35.0%
CGGTACGTAACATAAACATA+AGG - chr1_2:54063040-54063059 None:intergenic 35.0%
TCAAATTCTTAGTGCGTGTT+TGG + chr1_2:54063130-54063149 Msa0077230:intron 35.0%
TACATTGAAGCAAGAAAGGT+TGG - chr1_2:54063513-54063532 None:intergenic 35.0%
GTTGTACATTGAAGCAAGAA+AGG - chr1_2:54063517-54063536 None:intergenic 35.0%
CACTGCAATAAAGAAGGTAA+GGG - chr1_2:54063550-54063569 None:intergenic 35.0%
AGGTCCTTTGTATAATGTCA+TGG - chr1_2:54063791-54063810 None:intergenic 35.0%
GTGCTTCTATCAATAAGAGT+TGG + chr1_2:54063857-54063876 Msa0077230:CDS 35.0%
GATAAAGGGTTCACTTCTTA+CGG + chr1_2:54063879-54063898 Msa0077230:CDS 35.0%
AAGTATATCCAACTTGGGTT+AGG - chr1_2:54062973-54062992 None:intergenic 35.0%
CGGTACGTAACATAAACATA+AGG - chr1_2:54063040-54063059 None:intergenic 35.0%
TCAAATTCTTAGTGCGTGTT+TGG + chr1_2:54063130-54063149 Msa0077230:intron 35.0%
TACATTGAAGCAAGAAAGGT+TGG - chr1_2:54063513-54063532 None:intergenic 35.0%
GTTGTACATTGAAGCAAGAA+AGG - chr1_2:54063517-54063536 None:intergenic 35.0%
CACTGCAATAAAGAAGGTAA+GGG - chr1_2:54063550-54063569 None:intergenic 35.0%
AGGTCCTTTGTATAATGTCA+TGG - chr1_2:54063791-54063810 None:intergenic 35.0%
GTGCTTCTATCAATAAGAGT+TGG + chr1_2:54063857-54063876 Msa0077230:CDS 35.0%
GATAAAGGGTTCACTTCTTA+CGG + chr1_2:54063879-54063898 Msa0077230:CDS 35.0%
TGTGGCTTGAAATCTCTAAG+AGG - chr1_2:54062938-54062957 None:intergenic 40.0%
ACTGAAGCTTCCTTCAATCA+TGG + chr1_2:54063007-54063026 Msa0077230:CDS 40.0%
AGGACTCATTCCATGATTGA+AGG - chr1_2:54063020-54063039 None:intergenic 40.0%
AAAAGGAAACATCACAACCC+CGG - chr1_2:54063074-54063093 None:intergenic 40.0%
! GTTTCCTTTTGCGTACTTTC+TGG + chr1_2:54063084-54063103 Msa0077230:CDS 40.0%
AAGAGAGCAAATGTCAGCTT+TGG - chr1_2:54063331-54063350 None:intergenic 40.0%
AAGCTGACATTTGCTCTCTT+TGG + chr1_2:54063331-54063350 Msa0077230:CDS 40.0%
AGCTGACATTTGCTCTCTTT+GGG + chr1_2:54063332-54063351 Msa0077230:CDS 40.0%
CCACTGCAATAAAGAAGGTA+AGG - chr1_2:54063551-54063570 None:intergenic 40.0%
CCTTACCTTCTTTATTGCAG+TGG + chr1_2:54063548-54063567 Msa0077230:CDS 40.0%
TAGCACCACTGCAATAAAGA+AGG - chr1_2:54063556-54063575 None:intergenic 40.0%
! TCTTTATTGCAGTGGTGCTA+AGG + chr1_2:54063556-54063575 Msa0077230:CDS 40.0%
CACAGAGTTGACTAAAATGC+AGG + chr1_2:54063686-54063705 Msa0077230:intron 40.0%
!! GTGGCATAGCAAAAGTACTT+GGG + chr1_2:54063737-54063756 Msa0077230:CDS 40.0%
TGTGGCTTGAAATCTCTAAG+AGG - chr1_2:54062938-54062957 None:intergenic 40.0%
ACTGAAGCTTCCTTCAATCA+TGG + chr1_2:54063007-54063026 Msa0077230:CDS 40.0%
AGGACTCATTCCATGATTGA+AGG - chr1_2:54063020-54063039 None:intergenic 40.0%
AAAAGGAAACATCACAACCC+CGG - chr1_2:54063074-54063093 None:intergenic 40.0%
! GTTTCCTTTTGCGTACTTTC+TGG + chr1_2:54063084-54063103 Msa0077230:CDS 40.0%
AAGAGAGCAAATGTCAGCTT+TGG - chr1_2:54063331-54063350 None:intergenic 40.0%
AAGCTGACATTTGCTCTCTT+TGG + chr1_2:54063331-54063350 Msa0077230:CDS 40.0%
AGCTGACATTTGCTCTCTTT+GGG + chr1_2:54063332-54063351 Msa0077230:CDS 40.0%
CCACTGCAATAAAGAAGGTA+AGG - chr1_2:54063551-54063570 None:intergenic 40.0%
CCTTACCTTCTTTATTGCAG+TGG + chr1_2:54063548-54063567 Msa0077230:CDS 40.0%
TAGCACCACTGCAATAAAGA+AGG - chr1_2:54063556-54063575 None:intergenic 40.0%
! TCTTTATTGCAGTGGTGCTA+AGG + chr1_2:54063556-54063575 Msa0077230:CDS 40.0%
CACAGAGTTGACTAAAATGC+AGG + chr1_2:54063686-54063705 Msa0077230:intron 40.0%
!! GTGGCATAGCAAAAGTACTT+GGG + chr1_2:54063737-54063756 Msa0077230:CDS 40.0%
! CTCTCCAGAAAGTACGCAAA+AGG - chr1_2:54063091-54063110 None:intergenic 45.0%
!! CTTTTGCGTACTTTCTGGAG+AGG + chr1_2:54063089-54063108 Msa0077230:CDS 45.0%
GCTGACATTTGCTCTCTTTG+GGG + chr1_2:54063333-54063352 Msa0077230:CDS 45.0%
! AGGTTGGAGAAGTATACTGC+AGG - chr1_2:54063497-54063516 None:intergenic 45.0%
GTTGATCTTCGAAATCCACG+TGG + chr1_2:54063718-54063737 Msa0077230:CDS 45.0%
!! AGTACTTTTGCTATGCCACG+TGG - chr1_2:54063736-54063755 None:intergenic 45.0%
!! CGTGGCATAGCAAAAGTACT+TGG + chr1_2:54063736-54063755 Msa0077230:CDS 45.0%
AACACCAGCTAAGGAGATCA+AGG - chr1_2:54063764-54063783 None:intergenic 45.0%
CATGGTCATAACACCAGCTA+AGG - chr1_2:54063773-54063792 None:intergenic 45.0%
! CTCTCCAGAAAGTACGCAAA+AGG - chr1_2:54063091-54063110 None:intergenic 45.0%
!! CTTTTGCGTACTTTCTGGAG+AGG + chr1_2:54063089-54063108 Msa0077230:CDS 45.0%
GCTGACATTTGCTCTCTTTG+GGG + chr1_2:54063333-54063352 Msa0077230:CDS 45.0%
! AGGTTGGAGAAGTATACTGC+AGG - chr1_2:54063497-54063516 None:intergenic 45.0%
GTTGATCTTCGAAATCCACG+TGG + chr1_2:54063718-54063737 Msa0077230:CDS 45.0%
!! AGTACTTTTGCTATGCCACG+TGG - chr1_2:54063736-54063755 None:intergenic 45.0%
!! CGTGGCATAGCAAAAGTACT+TGG + chr1_2:54063736-54063755 Msa0077230:CDS 45.0%
AACACCAGCTAAGGAGATCA+AGG - chr1_2:54063764-54063783 None:intergenic 45.0%
CATGGTCATAACACCAGCTA+AGG - chr1_2:54063773-54063792 None:intergenic 45.0%
CTCATGCTCCTAACCCAAGT+TGG + chr1_2:54062962-54062981 Msa0077230:CDS 50.0%
CTCATGCTCCTAACCCAAGT+TGG + chr1_2:54062962-54062981 Msa0077230:CDS 50.0%
! GTTAGGAGCATGAGCAGGTG+TGG - chr1_2:54062956-54062975 None:intergenic 55.0%
! CTTGGGTTAGGAGCATGAGC+AGG - chr1_2:54062961-54062980 None:intergenic 55.0%
GGGACCTTGATCTCCTTAGC+TGG + chr1_2:54063757-54063776 Msa0077230:CDS 55.0%
! GTTAGGAGCATGAGCAGGTG+TGG - chr1_2:54062956-54062975 None:intergenic 55.0%
! CTTGGGTTAGGAGCATGAGC+AGG - chr1_2:54062961-54062980 None:intergenic 55.0%
GGGACCTTGATCTCCTTAGC+TGG + chr1_2:54063757-54063776 Msa0077230:CDS 55.0%
! ACGTACCGGCACGTTGTTGC+CGG + chr1_2:54063052-54063071 Msa0077230:CDS 60.0%
! ACGTACCGGCACGTTGTTGC+CGG + chr1_2:54063052-54063071 Msa0077230:CDS 60.0%
! CGTACCGGCACGTTGTTGCC+GGG + chr1_2:54063053-54063072 Msa0077230:CDS 65.0%
! GTACCGGCACGTTGTTGCCG+GGG + chr1_2:54063054-54063073 Msa0077230:CDS 65.0%
CAACCCCGGCAACAACGTGC+CGG - chr1_2:54063060-54063079 None:intergenic 65.0%
! CGTACCGGCACGTTGTTGCC+GGG + chr1_2:54063053-54063072 Msa0077230:CDS 65.0%
! GTACCGGCACGTTGTTGCCG+GGG + chr1_2:54063054-54063073 Msa0077230:CDS 65.0%
CAACCCCGGCAACAACGTGC+CGG - chr1_2:54063060-54063079 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 54062887 54063966 54062887 ID=Msa0077230;Name=Msa0077230
chr1_2 mRNA 54062887 54063966 54062887 ID=Msa0077230-mRNA-1;Parent=Msa0077230;Name=Msa0077230-mRNA-1;_AED=0.02;_eAED=0.02;_QI=27|1|0.75|1|1|1|4|0|211
chr1_2 exon 54062887 54063110 54062887 ID=Msa0077230-mRNA-1:exon:16650;Parent=Msa0077230-mRNA-1
chr1_2 exon 54063318 54063380 54063318 ID=Msa0077230-mRNA-1:exon:16651;Parent=Msa0077230-mRNA-1
chr1_2 exon 54063461 54063577 54063461 ID=Msa0077230-mRNA-1:exon:16652;Parent=Msa0077230-mRNA-1
chr1_2 exon 54063708 54063966 54063708 ID=Msa0077230-mRNA-1:exon:16653;Parent=Msa0077230-mRNA-1
chr1_2 five_prime_UTR 54062887 54062913 54062887 ID=Msa0077230-mRNA-1:five_prime_utr;Parent=Msa0077230-mRNA-1
chr1_2 CDS 54062914 54063110 54062914 ID=Msa0077230-mRNA-1:cds;Parent=Msa0077230-mRNA-1
chr1_2 CDS 54063318 54063380 54063318 ID=Msa0077230-mRNA-1:cds;Parent=Msa0077230-mRNA-1
chr1_2 CDS 54063461 54063577 54063461 ID=Msa0077230-mRNA-1:cds;Parent=Msa0077230-mRNA-1
chr1_2 CDS 54063708 54063966 54063708 ID=Msa0077230-mRNA-1:cds;Parent=Msa0077230-mRNA-1
Gene Sequence

>Msa0077230

ATGGAGCAAAAAATATTTTCTCCTCTTAGAGATTTCAAGCCACACCTGCTCATGCTCCTAACCCAAGTTGGATATACTTTAACATATTTTATCACTGAAGCTTCCTTCAATCATGGAATGAGTCCTTATGTTTATGTTACGTACCGGCACGTTGTTGCCGGGGTTGTGATGTTTCCTTTTGCGTACTTTCTGGAGAGAAGTGTAAGACCAAAGCTGACATTTGCTCTCTTTGGGGAGATTTTTGTTCTTTCAGTGTTAGGGATCAGTTTGTCTATCAATTTGTACTTTGCAAGCCTGCAGTATACTTCTCCAACCTTTCTTGCTTCAATGTACAACACTATTGCTTCCCTTACCTTCTTTATTGCAGTGGTGCTAAGGTTTGAGATTGTTGATCTTCGAAATCCACGTGGCATAGCAAAAGTACTTGGGACCTTGATCTCCTTAGCTGGTGTTATGACCATGACATTATACAAAGGACCTTTAATGAGAAATTTGTGGGATCCTCTAATCCATATTCAACCAAAAAGTGCTTCTATCAATAAGAGTTGGATAAAGGGTTCACTTCTTACGGTTACATTTTGTGTCACAATTTCTATATCGTACATTATGCAGGTTTTCACTTTAACTCATGACTAG
Protein sequence

>Msa0077230

MEQKIFSPLRDFKPHLLMLLTQVGYTLTYFITEASFNHGMSPYVYVTYRHVVAGVVMFPFAYFLERSVRPKLTFALFGEIFVLSVLGISLSINLYFASLQYTSPTFLASMYNTIASLTFFIAVVLRFEIVDLRNPRGIAKVLGTLISLAGVMTMTLYKGPLMRNLWDPLIHIQPKSASINKSWIKGSLLTVTFCVTISISYIMQVFTLTHD*