AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0102400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0102400 MtrunA17_Chr1g0156591 100.000 142 0 0 1 142 1 142 7.55e-102 287
Msa0102400 MtrunA17_Chr5g0448531 55.319 141 63 0 1 141 1 141 1.92e-54 168
Msa0102400 MtrunA17_Chr7g0263061 46.763 139 74 0 2 140 3 141 1.59e-38 127
Msa0102400 MtrunA17_Chr8g0389621 46.763 139 74 0 2 140 3 141 1.59e-38 127
Msa0102400 MtrunA17_Chr5g0440601 52.778 108 51 0 34 141 11 118 3.54e-37 124
Msa0102400 MtrunA17_Chr8g0373311 39.597 149 81 3 1 142 1 147 3.56e-30 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0102400 AT1G47830.1 94.366 142 8 0 1 142 1 142 2.83e-96 273
Msa0102400 AT4G35410.2 54.610 141 64 0 1 141 1 141 1.44e-53 166
Msa0102400 AT2G17380.1 53.901 141 65 0 1 141 1 141 1.72e-53 166
Msa0102400 AT2G19790.1 46.763 139 74 0 2 140 3 141 1.20e-38 128
Msa0102400 AT4G35410.1 53.061 98 46 0 1 98 1 98 4.50e-33 112
Msa0102400 AT3G50860.1 38.776 147 85 1 1 142 1 147 1.03e-32 114
Msa0102400 AT3G50860.2 37.931 145 85 1 1 140 1 145 1.50e-31 110

Find 84 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAACGAAATTAGTGTATT+TGG 0.082600 1_3:-9152814 Msa0102410:three_prime_UTR
ATTCTATATGTGAAGAAATT+AGG 0.140891 1_3:-9155736 Msa0102410:exon
TTTGATTTGAATTGCGATTA+TGG 0.202006 1_3:-9152568 Msa0102410:three_prime_UTR
CCATTTGTTTGTTGAAATCT+TGG 0.225064 1_3:+9153775 Msa0102400:CDS
GAACACACAGATAATGAAAA+AGG 0.229430 1_3:-9155531 Msa0102410:three_prime_UTR
TTCAAGAGGAACATAGTATT+TGG 0.241775 1_3:-9152733 Msa0102410:three_prime_UTR
CCTACATCACAAGAAATAAT+TGG 0.264657 1_3:-9155840 Msa0102410:CDS
CAATGTCTGCGAACTTGATT+TGG 0.278261 1_3:+9153811 Msa0102400:CDS
CAGAATCCTAATTGGCAAAA+TGG 0.286029 1_3:-9155921 Msa0102410:CDS
CAATTATATGGAACTCAATA+TGG 0.313814 1_3:-9156041 Msa0102410:CDS
TTCTATATGTGAAGAAATTA+GGG 0.352386 1_3:-9155735 Msa0102410:exon
TCAACGAAATTAGTGTATTT+GGG 0.354296 1_3:-9152813 Msa0102410:three_prime_UTR
CGTGGACCCAGAATCCTAAT+TGG 0.355576 1_3:-9155929 Msa0102410:CDS
GAATGAATTCATCAAGTATA+AGG 0.361705 1_3:-9155007 Msa0102410:intron
GTTGTAGACATTCAGAAAGT+TGG 0.365354 1_3:+9155495 Msa0102400:three_prime_UTR
GTATTCGACCTTGTGTTTCT+CGG 0.367660 1_3:-9152760 Msa0102410:three_prime_UTR
CCAATTATTTCTTGTGATGT+AGG 0.376828 1_3:+9155840 Msa0102400:three_prime_UTR
TTGTAGACATTCAGAAAGTT+GGG 0.378542 1_3:+9155496 Msa0102400:three_prime_UTR
GGGTATGAGGAAAGATGATT+TGG 0.386388 1_3:+9155862 Msa0102400:three_prime_UTR
TCTTGCCCATTTGTCTTGGA+TGG 0.392860 1_3:+9155770 Msa0102400:three_prime_UTR
TCTTGTGATGTAGGGGTATG+AGG 0.395732 1_3:+9155849 Msa0102400:three_prime_UTR
TCCAACGATTGAAGTAATCT+TGG 0.396123 1_3:+9155815 Msa0102400:three_prime_UTR
TGATGATTTACTATTGCAAT+TGG 0.398465 1_3:+9155367 Msa0102400:three_prime_UTR
AACATAGTATTTGGCTAAAC+GGG 0.399817 1_3:-9152724 Msa0102410:three_prime_UTR
CTTGCCCATTTGTCTTGGAT+GGG 0.417595 1_3:+9155771 Msa0102400:three_prime_UTR
CAATTATTTCTTGTGATGTA+GGG 0.418076 1_3:+9155841 Msa0102400:three_prime_UTR
CAGGCTATCATTGAAAGAAT+GGG 0.419258 1_3:+9155285 Msa0102400:intron
GAACATAGTATTTGGCTAAA+CGG 0.419670 1_3:-9152725 Msa0102410:three_prime_UTR
GGTATGAGGAAAGATGATTT+GGG 0.428294 1_3:+9155863 Msa0102400:three_prime_UTR
GCAGGCTATCATTGAAAGAA+TGG 0.433845 1_3:+9155284 Msa0102400:intron
ATCTTATTACACCAGTACTT+TGG 0.438490 1_3:+9155705 Msa0102400:three_prime_UTR
ATTATACAGTTCTATATCTC+TGG 0.448782 1_3:-9155596 Msa0102410:three_prime_UTR
CCAAGATTTCAACAAACAAA+TGG 0.450421 1_3:-9153775 Msa0102410:three_prime_UTR
TTGCCCATTTGTCTTGGATG+GGG 0.457853 1_3:+9155772 Msa0102400:three_prime_UTR
GTAATATACCGGAGATATGC+TGG 0.461155 1_3:+9153692 Msa0102400:CDS
CATTCTGTTGCAGAACAGAC+AGG 0.470525 1_3:+9152695 Msa0102400:CDS
AAGATCTTGCCCATTTGTCT+TGG 0.472158 1_3:+9155766 Msa0102400:three_prime_UTR
TAATCGCAATTCAAATCAAA+TGG 0.474191 1_3:+9152571 Msa0102400:exon
TGTATCACTCATTGTGAGAC+GGG 0.493371 1_3:-9155628 Msa0102410:three_prime_UTR
AGAAATGACTATTCTGCAAT+GGG 0.495928 1_3:-9155897 Msa0102410:CDS
TCCAAGATTACTTCAATCGT+TGG 0.497011 1_3:-9155816 Msa0102410:CDS
ATGTATTATGTACACTTCCA+TGG 0.500745 1_3:+9155449 Msa0102400:three_prime_UTR
TTTGAAAGTGAACAAAAGTA+AGG 0.502341 1_3:+9152460 Msa0102400:five_prime_UTR
AGGGACAGATTCCAAAGTAC+TGG 0.510784 1_3:-9155716 Msa0102410:three_prime_UTR
CTTGATGAATTCATTCTAGC+GGG 0.516282 1_3:+9155014 Msa0102400:CDS
TGTTCTGCAACAGAATGAAT+CGG 0.534099 1_3:-9152689 Msa0102410:intron
AAGAAATGACTATTCTGCAA+TGG 0.540060 1_3:-9155898 Msa0102410:CDS
AAGAAGATTTGAACAACGGA+TGG 0.540666 1_3:-9152513 Msa0102410:three_prime_UTR
AAAACAGTCCAGCATATCTC+CGG 0.546650 1_3:-9153700 Msa0102410:three_prime_UTR
TGCCCATTTGTCTTGGATGG+GGG 0.548139 1_3:+9155773 Msa0102400:three_prime_UTR
CATTTCAGCAGAGTCACGAC+AGG 0.554284 1_3:+9155337 Msa0102400:three_prime_UTR
GTATGAGGAAAGATGATTTG+GGG 0.565026 1_3:+9155864 Msa0102400:three_prime_UTR
TGTACATAATACATGGCTCA+AGG 0.566144 1_3:-9155440 Msa0102410:three_prime_UTR
AATTATTTCTTGTGATGTAG+GGG 0.566909 1_3:+9155842 Msa0102400:three_prime_UTR
TGCAACAAGCAGCTCAAGCT+AGG 0.574393 1_3:-9155974 Msa0102410:CDS
CATAATACATGGCTCAAGGG+TGG 0.577888 1_3:-9155436 Msa0102410:three_prime_UTR
TGTTTCTCGGAATCTTCAAG+AGG 0.587461 1_3:-9152747 Msa0102410:three_prime_UTR
CTGTAAATTGCAATATTACG+TGG 0.599616 1_3:-9155947 Msa0102410:CDS
ACACGAGATTTAAGAAACCA+TGG 0.601898 1_3:-9155466 Msa0102410:three_prime_UTR
ACTTGATGAATTCATTCTAG+CGG 0.603094 1_3:+9155013 Msa0102400:CDS
GAAGAAGAAGATTTGAACAA+CGG 0.603244 1_3:-9152517 Msa0102410:three_prime_UTR
TATGAGGAAAGATGATTTGG+GGG 0.607071 1_3:+9155865 Msa0102400:three_prime_UTR
TGTAGACATTCAGAAAGTTG+GGG 0.610223 1_3:+9155497 Msa0102400:three_prime_UTR
ATAATACATGGCTCAAGGGT+GGG 0.610710 1_3:-9155435 Msa0102410:three_prime_UTR
GTACATAATACATGGCTCAA+GGG 0.615332 1_3:-9155439 Msa0102410:three_prime_UTR
CAAATACACTAATTTCGTTG+AGG 0.615858 1_3:+9152815 Msa0102400:CDS
GTACACACAAGGTAATATAC+CGG 0.616996 1_3:+9153681 Msa0102400:CDS
GTGTATCACTCATTGTGAGA+CGG 0.617358 1_3:-9155629 Msa0102410:three_prime_UTR
GGAGTACGACGACGTTATTG+TGG 0.617611 1_3:-9152492 Msa0102410:three_prime_UTR
GAAACACAAGGTCGAATACG+AGG 0.625603 1_3:+9152764 Msa0102400:CDS
ATGGAAGTGTACATAATACA+TGG 0.629812 1_3:-9155447 Msa0102410:three_prime_UTR
CTAACTACAAAGAAGCACAA+TGG 0.630559 1_3:-9155566 Msa0102410:three_prime_UTR
ACTGCAAGAAACAAGCAAGA+AGG 0.630829 1_3:+9155040 Msa0102400:CDS
TGGGATCTCTATTTACGACA+AGG 0.631018 1_3:-9152794 Msa0102410:three_prime_UTR
GTTGCAGTTTCGTACACACA+AGG 0.632236 1_3:+9153670 Msa0102400:intron
AGGCTATCATTGAAAGAATG+GGG 0.635618 1_3:+9155286 Msa0102400:intron
ATTCTGTTGCAGAACAGACA+GGG 0.638809 1_3:+9152696 Msa0102400:CDS
TGACATCACTGATAATGAGT+TGG 0.649266 1_3:+9153736 Msa0102400:CDS
TGAAGATTCCGAGAAACACA+AGG 0.650445 1_3:+9152752 Msa0102400:CDS
GGCTATCATTGAAAGAATGG+GGG 0.667109 1_3:+9155287 Msa0102400:intron
GTATCACTCATTGTGAGACG+GGG 0.677728 1_3:-9155627 Msa0102410:three_prime_UTR
ATTTCAGCAGAGTCACGACA+GGG 0.688002 1_3:+9155338 Msa0102400:three_prime_UTR
TATCACTCATTGTGAGACGG+GGG 0.725206 1_3:-9155626 Msa0102410:three_prime_UTR
ATGAGGAAAGATGATTTGGG+GGG 0.787407 1_3:+9155866 Msa0102400:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAATATGCATTATTTA+TGG - chr1_3:9152856-9152875 Msa0102410:intron 15.0%
!! TTAAATAATAACCGTTTAAT+GGG + chr1_3:9153639-9153658 Msa0102400:intron 15.0%
!! TTTATGATACAAAATAATAG+TGG + chr1_3:9154066-9154085 Msa0102400:intron 15.0%
!!! AAATAATAGTGGAATATAAA+AGG + chr1_3:9154077-9154096 Msa0102400:intron 15.0%
!!! AATAATAGTGGAATATAAAA+GGG + chr1_3:9154078-9154097 Msa0102400:intron 15.0%
!! GTTAAATAATAACCGTTTAA+TGG + chr1_3:9153638-9153657 Msa0102400:intron 20.0%
!!! TTTTTTAGAACTAAAACATG+TGG - chr1_3:9154304-9154323 Msa0102410:intron 20.0%
!!! AAGGACATTTTTCATATATA+TGG - chr1_3:9154503-9154522 Msa0102410:intron 20.0%
!!! TTTAGTTTTATCTGATTTGA+AGG + chr1_3:9154980-9154999 Msa0102400:intron 20.0%
!! TTCTATATGTGAAGAAATTA+GGG - chr1_3:9155738-9155757 Msa0102410:exon 20.0%
!! ATTCTATATGTGAAGAAATT+AGG - chr1_3:9155739-9155758 Msa0102410:exon 20.0%
! TTTGAAAGTGAACAAAAGTA+AGG + chr1_3:9152460-9152479 Msa0102400:five_prime_UTR 25.0%
!!! TTTGATTTGAATTGCGATTA+TGG - chr1_3:9152571-9152590 Msa0102410:three_prime_UTR 25.0%
! TAATCGCAATTCAAATCAAA+TGG + chr1_3:9152571-9152590 Msa0102400:exon 25.0%
!! ATAAATTAATCTCCCCTTTT+TGG + chr1_3:9152637-9152656 Msa0102400:intron 25.0%
! TCAACGAAATTAGTGTATTT+GGG - chr1_3:9152816-9152835 Msa0102410:three_prime_UTR 25.0%
! TAATGAGTTCGATACAAATA+TGG + chr1_3:9152906-9152925 Msa0102400:intron 25.0%
!! AGATTTTCAACCAATTAACT+AGG - chr1_3:9153350-9153369 Msa0102410:intron 25.0%
! AACAAATGTTACCCATTAAA+CGG - chr1_3:9153653-9153672 Msa0102410:intron 25.0%
! CATGATTACTGAAAAACAAA+AGG - chr1_3:9153876-9153895 Msa0102410:intron 25.0%
!!! TTGTTTTTCAGTAATCATGT+AGG + chr1_3:9153877-9153896 Msa0102400:intron 25.0%
!!! TGTTTTTCAGTAATCATGTA+GGG + chr1_3:9153878-9153897 Msa0102400:intron 25.0%
! TGCTTAGATATTCAAAACAT+TGG + chr1_3:9153917-9153936 Msa0102400:intron 25.0%
! GTTATCTATCTTTGTATTGT+TGG + chr1_3:9153952-9153971 Msa0102400:intron 25.0%
! ATTTATAGAAAACGATGTCT+CGG + chr1_3:9154024-9154043 Msa0102400:intron 25.0%
! TTTAGATCAAAGTGGAAAAT+GGG - chr1_3:9154340-9154359 Msa0102410:intron 25.0%
! GCAATTGCAAATTATTTACT+AGG - chr1_3:9154468-9154487 Msa0102410:intron 25.0%
! AAAAATTTGTACACACAACA+AGG - chr1_3:9154558-9154577 Msa0102410:intron 25.0%
!!! TCACTTCTTTCATTTGTTTT+TGG + chr1_3:9154707-9154726 Msa0102400:intron 25.0%
! GTATGTTAAATTAGACACAA+TGG - chr1_3:9154789-9154808 Msa0102410:intron 25.0%
! GAATGAATTCATCAAGTATA+AGG - chr1_3:9155010-9155029 Msa0102410:three_prime_UTR 25.0%
! TGATGATTTACTATTGCAAT+TGG + chr1_3:9155367-9155386 Msa0102400:three_prime_UTR 25.0%
! AACACACAGATAATGAAAAA+GGG - chr1_3:9155533-9155552 Msa0102410:three_prime_UTR 25.0%
! ATTATACAGTTCTATATCTC+TGG - chr1_3:9155599-9155618 Msa0102410:three_prime_UTR 25.0%
!! CAATTATTTCTTGTGATGTA+GGG + chr1_3:9155841-9155860 Msa0102400:three_prime_UTR 25.0%
!! AATTATTTCTTGTGATGTAG+GGG + chr1_3:9155842-9155861 Msa0102400:three_prime_UTR 25.0%
GAAGAAGAAGATTTGAACAA+CGG - chr1_3:9152520-9152539 Msa0102410:three_prime_UTR 30.0%
AACATAGTATTTGGCTAAAC+GGG - chr1_3:9152727-9152746 Msa0102410:three_prime_UTR 30.0%
GAACATAGTATTTGGCTAAA+CGG - chr1_3:9152728-9152747 Msa0102410:three_prime_UTR 30.0%
TTCAAGAGGAACATAGTATT+TGG - chr1_3:9152736-9152755 Msa0102410:three_prime_UTR 30.0%
CTCAACGAAATTAGTGTATT+TGG - chr1_3:9152817-9152836 Msa0102410:three_prime_UTR 30.0%
CAAATACACTAATTTCGTTG+AGG + chr1_3:9152815-9152834 Msa0102400:CDS 30.0%
ACAATTTATCCCACTAAATG+GGG - chr1_3:9152979-9152998 Msa0102410:intron 30.0%
GACAATTTATCCCACTAAAT+GGG - chr1_3:9152980-9152999 Msa0102410:intron 30.0%
AATTAGTCCCTAACTTTGTA+GGG - chr1_3:9153081-9153100 Msa0102410:intron 30.0%
AAATTAGTCCCTAACTTTGT+AGG - chr1_3:9153082-9153101 Msa0102410:intron 30.0%
TACAAAGTTAGGGACTAATT+TGG + chr1_3:9153081-9153100 Msa0102400:intron 30.0%
TTGAAGGATTCCAAGTTAAT+TGG + chr1_3:9153171-9153190 Msa0102400:intron 30.0%
AATTACTGTTCACAGATGAT+GGG - chr1_3:9153510-9153529 Msa0102410:intron 30.0%
AAATTACTGTTCACAGATGA+TGG - chr1_3:9153511-9153530 Msa0102410:intron 30.0%
TGTGAACAGTAATTTATCTG+TGG + chr1_3:9153516-9153535 Msa0102400:intron 30.0%
! GAAGCTGTAGTTCTATATAT+TGG + chr1_3:9153596-9153615 Msa0102400:intron 30.0%
CCAAGATTTCAACAAACAAA+TGG - chr1_3:9153778-9153797 Msa0102410:three_prime_UTR 30.0%
! CCATTTGTTTGTTGAAATCT+TGG + chr1_3:9153775-9153794 Msa0102400:CDS 30.0%
!!! TTTGGTTTTTAACTTCCACA+AGG + chr1_3:9153829-9153848 Msa0102400:CDS 30.0%
TGACAAAATAACAAACCTTG+TGG - chr1_3:9153847-9153866 Msa0102410:intron 30.0%
!! GTTTTTCAGTAATCATGTAG+GGG + chr1_3:9153879-9153898 Msa0102400:intron 30.0%
! TGGTAATGCTATGATGATAT+TGG + chr1_3:9153972-9153991 Msa0102400:intron 30.0%
GGTAATGCTATGATGATATT+GGG + chr1_3:9153973-9153992 Msa0102400:intron 30.0%
AATATAAAAGGGTAGATGGA+TGG + chr1_3:9154089-9154108 Msa0102400:intron 30.0%
ATATAAAAGGGTAGATGGAT+GGG + chr1_3:9154090-9154109 Msa0102400:intron 30.0%
!!! GAAGAATCTGAGTGTTTTTT+AGG + chr1_3:9154130-9154149 Msa0102400:intron 30.0%
GCAAAAGTTGAAATACATGA+TGG + chr1_3:9154182-9154201 Msa0102400:intron 30.0%
TTAGATCAAAGTGGAAAATG+GGG - chr1_3:9154339-9154358 Msa0102410:intron 30.0%
CTTTAGATCAAAGTGGAAAA+TGG - chr1_3:9154341-9154360 Msa0102410:intron 30.0%
GAAGTTGGATCTAGTATTTA+CGG - chr1_3:9154435-9154454 Msa0102410:intron 30.0%
TTCATATATATGGCAGCAAA+TGG - chr1_3:9154493-9154512 Msa0102410:intron 30.0%
TATATGAAAAATGTCCTTGC+AGG + chr1_3:9154505-9154524 Msa0102400:intron 30.0%
GTGTCTAATTTAACATACAG+CGG + chr1_3:9154791-9154810 Msa0102400:intron 30.0%
CAGATAAAACTAAATGAGCA+AGG - chr1_3:9154974-9154993 Msa0102410:intron 30.0%
ACTTGATGAATTCATTCTAG+CGG + chr1_3:9155013-9155032 Msa0102400:CDS 30.0%
CATAGGTACCTTATGATTTA+AGG + chr1_3:9155125-9155144 Msa0102400:intron 30.0%
! AACACAAAACAGGTTAGTTA+AGG - chr1_3:9155263-9155282 Msa0102410:intron 30.0%
! ATGGAAGTGTACATAATACA+TGG - chr1_3:9155450-9155469 Msa0102410:three_prime_UTR 30.0%
ATGTATTATGTACACTTCCA+TGG + chr1_3:9155449-9155468 Msa0102400:three_prime_UTR 30.0%
TTGTAGACATTCAGAAAGTT+GGG + chr1_3:9155496-9155515 Msa0102400:three_prime_UTR 30.0%
GAACACACAGATAATGAAAA+AGG - chr1_3:9155534-9155553 Msa0102410:three_prime_UTR 30.0%
!!! CTTGCTCATTTCACTTTTTA+AGG + chr1_3:9155651-9155670 Msa0102400:three_prime_UTR 30.0%
ATCTTATTACACCAGTACTT+TGG + chr1_3:9155705-9155724 Msa0102400:three_prime_UTR 30.0%
CCTACATCACAAGAAATAAT+TGG - chr1_3:9155843-9155862 Msa0102410:CDS 30.0%
! CCAATTATTTCTTGTGATGT+AGG + chr1_3:9155840-9155859 Msa0102400:three_prime_UTR 30.0%
AGAAATGACTATTCTGCAAT+GGG - chr1_3:9155900-9155919 Msa0102410:CDS 30.0%
AAGAAATGACTATTCTGCAA+TGG - chr1_3:9155901-9155920 Msa0102410:CDS 30.0%
! TTTCTTCCATTTTGCCAATT+AGG + chr1_3:9155915-9155934 Msa0102400:three_prime_UTR 30.0%
! ATTTTGCCAATTAGGATTCT+GGG + chr1_3:9155923-9155942 Msa0102400:three_prime_UTR 30.0%
CTGTAAATTGCAATATTACG+TGG - chr1_3:9155950-9155969 Msa0102410:CDS 30.0%
AAGAAGATTTGAACAACGGA+TGG - chr1_3:9152516-9152535 Msa0102410:three_prime_UTR 35.0%
! TTAATCTCCCCTTTTTGGAT+TGG + chr1_3:9152642-9152661 Msa0102400:intron 35.0%
AACACGATCCAATCCAAAAA+GGG - chr1_3:9152653-9152672 Msa0102410:intron 35.0%
AAACACGATCCAATCCAAAA+AGG - chr1_3:9152654-9152673 Msa0102410:intron 35.0%
TGTTCTGCAACAGAATGAAT+CGG - chr1_3:9152692-9152711 Msa0102410:intron 35.0%
ACACTAATTTCGTTGAGGTA+TGG + chr1_3:9152820-9152839 Msa0102400:intron 35.0%
CGACAATTTATCCCACTAAA+TGG - chr1_3:9152981-9153000 Msa0102410:intron 35.0%
AAAGTTAGGGACTAATTTGG+TGG + chr1_3:9153084-9153103 Msa0102400:intron 35.0%
!! GTCATTTTCACGTGTGATAT+GGG - chr1_3:9153148-9153167 Msa0102410:intron 35.0%
!! AGTCATTTTCACGTGTGATA+TGG - chr1_3:9153149-9153168 Msa0102410:intron 35.0%
ACGTGAAAATGACTGTTTGA+AGG + chr1_3:9153155-9153174 Msa0102400:intron 35.0%
AGGATTCCAAGTTAATTGGT+TGG + chr1_3:9153175-9153194 Msa0102400:intron 35.0%
TGTTCTCCAACCAATTAACT+TGG - chr1_3:9153184-9153203 Msa0102410:intron 35.0%
AGGTCTAAGAAGGCAAAAAA+AGG - chr1_3:9153254-9153273 Msa0102410:intron 35.0%
TTAGACCTTACTGTTGTCAT+TGG + chr1_3:9153266-9153285 Msa0102400:intron 35.0%
ATAAAGGACATTCAAGGTCT+TGG - chr1_3:9153296-9153315 Msa0102410:intron 35.0%
CAATTAACTAGGACCTTTCA+AGG - chr1_3:9153339-9153358 Msa0102410:intron 35.0%
CTTGAAAGGTCCTAGTTAAT+TGG + chr1_3:9153337-9153356 Msa0102400:intron 35.0%
! AAATCTATCCGTGAAGCTTT+TGG + chr1_3:9153364-9153383 Msa0102400:intron 35.0%
! AATCTATCCGTGAAGCTTTT+GGG + chr1_3:9153365-9153384 Msa0102400:intron 35.0%
ATTTCATCCCAAAAGCTTCA+CGG - chr1_3:9153375-9153394 Msa0102410:intron 35.0%
TGATGGGAAAATCAAACATG+AGG - chr1_3:9153494-9153513 Msa0102410:intron 35.0%
!!! AGCATTCTGCTGTTTTAAAG+TGG + chr1_3:9153571-9153590 Msa0102400:intron 35.0%
GTACACACAAGGTAATATAC+CGG + chr1_3:9153681-9153700 Msa0102400:CDS 35.0%
! TGACATCACTGATAATGAGT+TGG + chr1_3:9153736-9153755 Msa0102400:CDS 35.0%
CTCGGAAATCGAGATAAATT+AGG + chr1_3:9154042-9154061 Msa0102400:intron 35.0%
GTGGAATATAAAAGGGTAGA+TGG + chr1_3:9154085-9154104 Msa0102400:intron 35.0%
GGTGTCAGAATTAGAATTAG+CGG + chr1_3:9154220-9154239 Msa0102400:intron 35.0%
GTGTCAGAATTAGAATTAGC+GGG + chr1_3:9154221-9154240 Msa0102400:intron 35.0%
TGTCAGAATTAGAATTAGCG+GGG + chr1_3:9154222-9154241 Msa0102400:intron 35.0%
TAGACTCACTATTCAACTGT+AGG + chr1_3:9154269-9154288 Msa0102400:intron 35.0%
AACAAGGCTTGTATTGAAGA+CGG - chr1_3:9154542-9154561 Msa0102410:intron 35.0%
!! AAGGTTGCTTTGGATCTTTT+GGG - chr1_3:9154642-9154661 Msa0102410:intron 35.0%
!! TAAGGTTGCTTTGGATCTTT+TGG - chr1_3:9154643-9154662 Msa0102410:intron 35.0%
AAGTGAGAATAAGGTTGCTT+TGG - chr1_3:9154652-9154671 Msa0102410:intron 35.0%
TGCTCAATTAGAAACTCAAC+AGG - chr1_3:9154747-9154766 Msa0102410:intron 35.0%
CAGCAATCACAAACTTCATT+TGG - chr1_3:9154870-9154889 Msa0102410:intron 35.0%
AATGAAGTTTGTGATTGCTG+AGG + chr1_3:9154870-9154889 Msa0102400:intron 35.0%
ATGAAGTTTGTGATTGCTGA+GGG + chr1_3:9154871-9154890 Msa0102400:intron 35.0%
CTTGATGAATTCATTCTAGC+GGG + chr1_3:9155014-9155033 Msa0102400:CDS 35.0%
! GACATGTGATTGCTAGTTTA+AGG + chr1_3:9155093-9155112 Msa0102400:intron 35.0%
CAGTTAGGCCTTAAATCATA+AGG - chr1_3:9155136-9155155 Msa0102410:intron 35.0%
TCGATTTCCTTTGCTTCTTT+GGG + chr1_3:9155156-9155175 Msa0102400:intron 35.0%
! ACAGGTTAGTTAAGGAATTG+TGG - chr1_3:9155255-9155274 Msa0102410:intron 35.0%
CAGGCTATCATTGAAAGAAT+GGG + chr1_3:9155285-9155304 Msa0102400:intron 35.0%
AGGCTATCATTGAAAGAATG+GGG + chr1_3:9155286-9155305 Msa0102400:intron 35.0%
GTACATAATACATGGCTCAA+GGG - chr1_3:9155442-9155461 Msa0102410:three_prime_UTR 35.0%
TGTACATAATACATGGCTCA+AGG - chr1_3:9155443-9155462 Msa0102410:three_prime_UTR 35.0%
ACACGAGATTTAAGAAACCA+TGG - chr1_3:9155469-9155488 Msa0102410:three_prime_UTR 35.0%
GTTGTAGACATTCAGAAAGT+TGG + chr1_3:9155495-9155514 Msa0102400:three_prime_UTR 35.0%
TGTAGACATTCAGAAAGTTG+GGG + chr1_3:9155497-9155516 Msa0102400:three_prime_UTR 35.0%
! CTAACTACAAAGAAGCACAA+TGG - chr1_3:9155569-9155588 Msa0102410:three_prime_UTR 35.0%
TCCAACGATTGAAGTAATCT+TGG + chr1_3:9155815-9155834 Msa0102400:three_prime_UTR 35.0%
TCCAAGATTACTTCAATCGT+TGG - chr1_3:9155819-9155838 Msa0102410:CDS 35.0%
GGTATGAGGAAAGATGATTT+GGG + chr1_3:9155863-9155882 Msa0102400:three_prime_UTR 35.0%
GTATGAGGAAAGATGATTTG+GGG + chr1_3:9155864-9155883 Msa0102400:three_prime_UTR 35.0%
! TATGAGGAAAGATGATTTGG+GGG + chr1_3:9155865-9155884 Msa0102400:three_prime_UTR 35.0%
CAGAATCCTAATTGGCAAAA+TGG - chr1_3:9155924-9155943 Msa0102410:CDS 35.0%
! CATTTTGCCAATTAGGATTC+TGG + chr1_3:9155922-9155941 Msa0102400:three_prime_UTR 35.0%
!! ACAAGAGCACCAAAAATTGT+GGG - chr1_3:9156003-9156022 Msa0102410:CDS 35.0%
!! AACAAGAGCACCAAAAATTG+TGG - chr1_3:9156004-9156023 Msa0102410:CDS 35.0%
ACACGATCCAATCCAAAAAG+GGG - chr1_3:9152652-9152671 Msa0102410:intron 40.0%
ATTCTGTTGCAGAACAGACA+GGG + chr1_3:9152696-9152715 Msa0102400:CDS 40.0%
TGTTTCTCGGAATCTTCAAG+AGG - chr1_3:9152750-9152769 Msa0102410:three_prime_UTR 40.0%
TGAAGATTCCGAGAAACACA+AGG + chr1_3:9152752-9152771 Msa0102400:CDS 40.0%
GTATTCGACCTTGTGTTTCT+CGG - chr1_3:9152763-9152782 Msa0102410:three_prime_UTR 40.0%
TGGGATCTCTATTTACGACA+AGG - chr1_3:9152797-9152816 Msa0102410:three_prime_UTR 40.0%
CAGCTACATGGATCAATCAA+AGG - chr1_3:9152955-9152974 Msa0102410:intron 40.0%
TCTGTTCTCTAACTCCTAGA+AGG - chr1_3:9153014-9153033 Msa0102410:intron 40.0%
! AGAGCTAAATCACGTTTTGC+TGG + chr1_3:9153210-9153229 Msa0102400:intron 40.0%
GACAACAGTAAGGTCTAAGA+AGG - chr1_3:9153264-9153283 Msa0102410:intron 40.0%
GATCTCCAATGACAACAGTA+AGG - chr1_3:9153274-9153293 Msa0102410:intron 40.0%
! GGAAGCATAAAGGACATTCA+AGG - chr1_3:9153302-9153321 Msa0102410:intron 40.0%
GATGCACGGTTACTAAACAA+CGG - chr1_3:9153445-9153464 Msa0102410:intron 40.0%
TGGGAGAAAAAACTGATGCA+CGG - chr1_3:9153459-9153478 Msa0102410:intron 40.0%
CATGAGGAAGCAAAAGAGAT+GGG - chr1_3:9153478-9153497 Msa0102410:intron 40.0%
ACATGAGGAAGCAAAAGAGA+TGG - chr1_3:9153479-9153498 Msa0102410:intron 40.0%
GTAATATACCGGAGATATGC+TGG + chr1_3:9153692-9153711 Msa0102400:CDS 40.0%
AAAACAGTCCAGCATATCTC+CGG - chr1_3:9153703-9153722 Msa0102410:three_prime_UTR 40.0%
CAATGTCTGCGAACTTGATT+TGG + chr1_3:9153811-9153830 Msa0102400:CDS 40.0%
!! GATGCTGCTTTAGATCAAAG+TGG - chr1_3:9154348-9154367 Msa0102410:intron 40.0%
CTAGGTCTTCTACAAGAAGT+TGG - chr1_3:9154450-9154469 Msa0102410:intron 40.0%
! CGGAATCAAATTTTCCTGCA+AGG - chr1_3:9154522-9154541 Msa0102410:intron 40.0%
!! AGGTTGCTTTGGATCTTTTG+GGG - chr1_3:9154641-9154660 Msa0102410:intron 40.0%
CGTAGTGAACAGTAAGACTT+AGG - chr1_3:9154832-9154851 Msa0102410:intron 40.0%
ACTGCAAGAAACAAGCAAGA+AGG + chr1_3:9155040-9155059 Msa0102400:CDS 40.0%
AGCAAAGGAAATCGACAGTT+AGG - chr1_3:9155151-9155170 Msa0102410:intron 40.0%
! GTCGATTTCCTTTGCTTCTT+TGG + chr1_3:9155155-9155174 Msa0102400:intron 40.0%
AAATGTGCCCAAAGAAGCAA+AGG - chr1_3:9155166-9155185 Msa0102410:intron 40.0%
TTCTCTGCTTACGACAATTG+CGG + chr1_3:9155184-9155203 Msa0102400:intron 40.0%
! CTAACCTGTTTTGTGTTTGC+AGG + chr1_3:9155266-9155285 Msa0102400:intron 40.0%
ATAGCCTGCAAACACAAAAC+AGG - chr1_3:9155273-9155292 Msa0102410:intron 40.0%
GCAGGCTATCATTGAAAGAA+TGG + chr1_3:9155284-9155303 Msa0102400:intron 40.0%
GGCTATCATTGAAAGAATGG+GGG + chr1_3:9155287-9155306 Msa0102400:intron 40.0%
ATAATACATGGCTCAAGGGT+GGG - chr1_3:9155438-9155457 Msa0102410:three_prime_UTR 40.0%
TGTATCACTCATTGTGAGAC+GGG - chr1_3:9155631-9155650 Msa0102410:three_prime_UTR 40.0%
GTGTATCACTCATTGTGAGA+CGG - chr1_3:9155632-9155651 Msa0102410:three_prime_UTR 40.0%
AAGATCTTGCCCATTTGTCT+TGG + chr1_3:9155766-9155785 Msa0102400:three_prime_UTR 40.0%
GGGTATGAGGAAAGATGATT+TGG + chr1_3:9155862-9155881 Msa0102400:three_prime_UTR 40.0%
! ATGAGGAAAGATGATTTGGG+GGG + chr1_3:9155866-9155885 Msa0102400:three_prime_UTR 40.0%
!! AAGAGCACCAAAAATTGTGG+GGG - chr1_3:9156001-9156020 Msa0102410:CDS 40.0%
!! CAAGAGCACCAAAAATTGTG+GGG - chr1_3:9156002-9156021 Msa0102410:CDS 40.0%
CATTCTGTTGCAGAACAGAC+AGG + chr1_3:9152695-9152714 Msa0102400:CDS 45.0%
GAAACACAAGGTCGAATACG+AGG + chr1_3:9152764-9152783 Msa0102400:CDS 45.0%
ACTAAATGGGGTCAGCTACA+TGG - chr1_3:9152967-9152986 Msa0102410:intron 45.0%
ATGTAGCTGACCCCATTTAG+TGG + chr1_3:9152966-9152985 Msa0102400:intron 45.0%
TGTAGCTGACCCCATTTAGT+GGG + chr1_3:9152967-9152986 Msa0102400:intron 45.0%
GTCGTTGTTGTAGTCCTTCT+AGG + chr1_3:9152997-9153016 Msa0102400:intron 45.0%
!! TGAGAGTGCCCTACAAAGTT+AGG + chr1_3:9153070-9153089 Msa0102400:intron 45.0%
!! GAGAGTGCCCTACAAAGTTA+GGG + chr1_3:9153071-9153090 Msa0102400:intron 45.0%
AGGACCTTTCAAGGAAAACG+CGG - chr1_3:9153330-9153349 Msa0102410:intron 45.0%
GTTGCAGTTTCGTACACACA+AGG + chr1_3:9153670-9153689 Msa0102400:intron 45.0%
TAAAAGGGTAGATGGATGGG+TGG + chr1_3:9154093-9154112 Msa0102400:intron 45.0%
TGGATGGGTGGATATGGAAT+AGG + chr1_3:9154105-9154124 Msa0102400:intron 45.0%
GGATGGGTGGATATGGAATA+GGG + chr1_3:9154106-9154125 Msa0102400:intron 45.0%
TGATGGCACAAAAAGAGCTG+AGG + chr1_3:9154199-9154218 Msa0102400:intron 45.0%
CAAAGTGGAAAATGGGGCAA+AGG - chr1_3:9154333-9154352 Msa0102410:intron 45.0%
ACTGCAGCGAAGTGAGAATA+AGG - chr1_3:9154661-9154680 Msa0102410:intron 45.0%
AGTTTGTGATTGCTGAGGGT+TGG + chr1_3:9154875-9154894 Msa0102400:intron 45.0%
GTTTGTGATTGCTGAGGGTT+GGG + chr1_3:9154876-9154895 Msa0102400:intron 45.0%
! GTTTAAGGCTTGAGCTGCAT+AGG + chr1_3:9155108-9155127 Msa0102400:intron 45.0%
ATTTCAGCAGAGTCACGACA+GGG + chr1_3:9155338-9155357 Msa0102400:three_prime_UTR 45.0%
CATAATACATGGCTCAAGGG+TGG - chr1_3:9155439-9155458 Msa0102410:three_prime_UTR 45.0%
TATCACTCATTGTGAGACGG+GGG - chr1_3:9155629-9155648 Msa0102410:three_prime_UTR 45.0%
GTATCACTCATTGTGAGACG+GGG - chr1_3:9155630-9155649 Msa0102410:three_prime_UTR 45.0%
AGGGACAGATTCCAAAGTAC+TGG - chr1_3:9155719-9155738 Msa0102410:three_prime_UTR 45.0%
TCTTGCCCATTTGTCTTGGA+TGG + chr1_3:9155770-9155789 Msa0102400:three_prime_UTR 45.0%
CTTGCCCATTTGTCTTGGAT+GGG + chr1_3:9155771-9155790 Msa0102400:three_prime_UTR 45.0%
TTGCCCATTTGTCTTGGATG+GGG + chr1_3:9155772-9155791 Msa0102400:three_prime_UTR 45.0%
TTCCCCCATCCAAGACAAAT+GGG - chr1_3:9155778-9155797 Msa0102410:CDS 45.0%
TTTCCCCCATCCAAGACAAA+TGG - chr1_3:9155779-9155798 Msa0102410:CDS 45.0%
! TCTTGTGATGTAGGGGTATG+AGG + chr1_3:9155849-9155868 Msa0102400:three_prime_UTR 45.0%
! GTTGCATCCCCCACAATTTT+TGG + chr1_3:9155991-9156010 Msa0102400:three_prime_UTR 45.0%
!! TGATTTAATATATTATAAAT+TGG + chr1_3:9154246-9154265 Msa0102400:intron 5.0%
GGAGTACGACGACGTTATTG+TGG - chr1_3:9152495-9152514 Msa0102410:three_prime_UTR 50.0%
CCTTTCAAGGAAAACGCGGA+GGG - chr1_3:9153326-9153345 Msa0102410:intron 50.0%
ACCTTTCAAGGAAAACGCGG+AGG - chr1_3:9153327-9153346 Msa0102410:intron 50.0%
GGTAGATGGATGGGTGGATA+TGG + chr1_3:9154099-9154118 Msa0102400:intron 50.0%
!! AGGAGTGCCACATCAGAAGA+TGG + chr1_3:9154150-9154169 Msa0102400:intron 50.0%
GTGCCACATCAGAAGATGGT+CGG + chr1_3:9154154-9154173 Msa0102400:intron 50.0%
CATCCGACCATCTTCTGATG+TGG - chr1_3:9154160-9154179 Msa0102410:intron 50.0%
CATCAGAAGATGGTCGGATG+TGG + chr1_3:9154160-9154179 Msa0102400:intron 50.0%
CATTTCAGCAGAGTCACGAC+AGG + chr1_3:9155337-9155356 Msa0102400:three_prime_UTR 50.0%
TGCCCATTTGTCTTGGATGG+GGG + chr1_3:9155773-9155792 Msa0102400:three_prime_UTR 50.0%
! AAGATGATTTGGGGGGCACA+TGG + chr1_3:9155873-9155892 Msa0102400:three_prime_UTR 50.0%
CGTGGACCCAGAATCCTAAT+TGG - chr1_3:9155932-9155951 Msa0102410:CDS 50.0%
TGCAACAAGCAGCTCAAGCT+AGG - chr1_3:9155977-9155996 Msa0102410:CDS 50.0%
TTCAAGGAAAACGCGGAGGG+AGG - chr1_3:9153323-9153342 Msa0102410:intron 55.0%
! CCCTCCGCGTTTTCCTTGAA+AGG + chr1_3:9153323-9153342 Msa0102400:intron 55.0%
GTGATTGCTGAGGGTTGGGT+TGG + chr1_3:9154880-9154899 Msa0102400:intron 55.0%
! CGCGGAGGGAGGAAGCATAA+AGG - chr1_3:9153312-9153331 Msa0102410:intron 60.0%
!! TGCTGAGGGTTGGGTTGGAG+TGG + chr1_3:9154885-9154904 Msa0102400:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 9152453 9156057 9152453 ID=Msa0102400;Name=Msa0102400
chr1_3 mRNA 9152453 9156057 9152453 ID=Msa0102400-mRNA-1;Parent=Msa0102400;Name=Msa0102400-mRNA-1;_AED=0.07;_eAED=0.07;_QI=137|1|1|1|0.75|0.8|5|728|142
chr1_3 exon 9152453 9152592 9152453 ID=Msa0102400-mRNA-1:exon:3670;Parent=Msa0102400-mRNA-1
chr1_3 exon 9152687 9152836 9152687 ID=Msa0102400-mRNA-1:exon:3671;Parent=Msa0102400-mRNA-1
chr1_3 exon 9153677 9153850 9153677 ID=Msa0102400-mRNA-1:exon:3672;Parent=Msa0102400-mRNA-1
chr1_3 exon 9155002 9155061 9155002 ID=Msa0102400-mRNA-1:exon:3673;Parent=Msa0102400-mRNA-1
chr1_3 exon 9155288 9156057 9155288 ID=Msa0102400-mRNA-1:exon:3674;Parent=Msa0102400-mRNA-1
chr1_3 five_prime_UTR 9152453 9152589 9152453 ID=Msa0102400-mRNA-1:five_prime_utr;Parent=Msa0102400-mRNA-1
chr1_3 CDS 9152590 9152592 9152590 ID=Msa0102400-mRNA-1:cds;Parent=Msa0102400-mRNA-1
chr1_3 CDS 9152687 9152836 9152687 ID=Msa0102400-mRNA-1:cds;Parent=Msa0102400-mRNA-1
chr1_3 CDS 9153677 9153850 9153677 ID=Msa0102400-mRNA-1:cds;Parent=Msa0102400-mRNA-1
chr1_3 CDS 9155002 9155061 9155002 ID=Msa0102400-mRNA-1:cds;Parent=Msa0102400-mRNA-1
chr1_3 CDS 9155288 9155329 9155288 ID=Msa0102400-mRNA-1:cds;Parent=Msa0102400-mRNA-1
chr1_3 three_prime_UTR 9155330 9156057 9155330 ID=Msa0102400-mRNA-1:three_prime_utr;Parent=Msa0102400-mRNA-1
Gene Sequence

>Msa0102400

ATGATCCGATTCATTCTGTTGCAGAACAGACAGGGTAAAACCCGTTTAGCCAAATACTATGTTCCTCTTGAAGATTCCGAGAAACACAAGGTCGAATACGAGGTTCATCGCCTTGTCGTAAATAGAGATCCCAAATACACTAATTTCGTTGAGTTTCGTACACACAAGGTAATATACCGGAGATATGCTGGACTGTTTTTCTCGCTTTGTGTTGACATCACTGATAATGAGTTGGCATATTTAGAGTGCATCCATTTGTTTGTTGAAATCTTGGATCACTTCTTCAGCAATGTCTGCGAACTTGATTTGGTTTTTAACTTCCACAAGGTCTACCTTATACTTGATGAATTCATTCTAGCGGGTGAACTGCAAGAAACAAGCAAGAAGGCTATCATTGAAAGAATGGGGGAGCTTGAAAAACTAGAGTGA
Protein sequence

>Msa0102400

MIRFILLQNRQGKTRLAKYYVPLEDSEKHKVEYEVHRLVVNRDPKYTNFVEFRTHKVIYRRYAGLFFSLCVDITDNELAYLECIHLFVEILDHFFSNVCELDLVFNFHKVYLILDEFILAGELQETSKKAIIERMGELEKLE*