AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0603750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603750 MtrunA17_Chr4g0032451 77.241 145 33 0 13 157 10 154 1.05e-77 228
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGAACCTCATTCTCAAAT+AGG 0.255937 4_2:-48415980 None:intergenic
CACAATTTGACCGCAATCTA+TGG 0.270659 4_2:-48416403 None:intergenic
AAAATGAGCTGAATCAATTT+AGG 0.306772 4_2:-48416767 None:intergenic
CATGAGAGAGGACGCGATTT+AGG 0.306895 4_2:-48416584 None:intergenic
CAGAGATAGAGGTAGCAAAA+TGG 0.323011 4_2:+48416672 Msa0603750:three_prime_UTR
TTACTGACCTCTTTACATAA+TGG 0.325529 4_2:-48416065 None:intergenic
GGAAGAGGATATGTCTCTTC+AGG 0.339040 4_2:+48415920 Msa0603750:CDS
ACAAGAACATATCCTTTAGA+AGG 0.368831 4_2:+48416529 Msa0603750:three_prime_UTR
ACCTCTATTGTCAATGCAAC+TGG 0.372040 4_2:-48416167 None:intergenic
AAACTACCGGAGCATCTTGC+AGG 0.376357 4_2:+48416041 Msa0603750:CDS
AACTGCCCTATTTGAGAATG+AGG 0.416735 4_2:+48415974 Msa0603750:CDS
TGAATTGATTGCAGCCGAAT+TGG 0.427925 4_2:+48415899 Msa0603750:CDS
GACTTAATTGAGGAACAATC+AGG 0.432812 4_2:+48416137 Msa0603750:CDS
GTGAACCTCATTCTCAAATA+GGG 0.436037 4_2:-48415979 None:intergenic
TCTTCAGGATTCATTGAGAA+AGG 0.440370 4_2:+48415935 Msa0603750:CDS
TTAGGGATTACCAGTTAAAA+CGG 0.449452 4_2:-48414134 None:intergenic
TTCAGAGGCGCAGAGCTACT+AGG 0.458273 4_2:-48414165 None:intergenic
CAATATTGATGACTTAATTG+AGG 0.464389 4_2:+48416127 Msa0603750:CDS
AGACATATCCTCTTCCAATT+CGG 0.468675 4_2:-48415913 None:intergenic
AGAGATAGAGGTAGCAAAAT+GGG 0.478511 4_2:+48416673 Msa0603750:three_prime_UTR
CAATCCACTGAAGATATGTA+AGG 0.478649 4_2:+48416443 Msa0603750:three_prime_UTR
CATCTATTAGGATAGTAAAG+TGG 0.499249 4_2:-48416829 None:intergenic
TAGGGATTACCAGTTAAAAC+GGG 0.499942 4_2:-48414133 None:intergenic
ATTTATCGATGAATTGTACA+TGG 0.501762 4_2:+48416217 Msa0603750:CDS
TTCCGAGAAGTCCATGGAAA+AGG 0.505472 4_2:+48415661 Msa0603750:CDS
AAATATGCATGCAGAGATAG+AGG 0.505997 4_2:+48416661 Msa0603750:three_prime_UTR
TTGAGTCGAAGTGTTGGACT+TGG 0.506935 4_2:+48416741 Msa0603750:three_prime_UTR
ATCGATGAATTGTACATGGA+TGG 0.519577 4_2:+48416221 Msa0603750:CDS
TGCAGGTCCATTATGTAAAG+AGG 0.525512 4_2:+48416058 Msa0603750:CDS
GATTGCAGCCGAATTGGAAG+AGG 0.533785 4_2:+48415905 Msa0603750:CDS
CAACATTTCCGAGAAGTCCA+TGG 0.547363 4_2:+48415655 Msa0603750:CDS
ATCTATTAGGATAGTAAAGT+GGG 0.548839 4_2:-48416828 None:intergenic
TGTCTGAGACAACCTTCTAA+AGG 0.551083 4_2:-48416541 None:intergenic
AATGGACCTGCAAGATGCTC+CGG 0.552350 4_2:-48416047 None:intergenic
CATAGATTGCGGTCAAATTG+TGG 0.553076 4_2:+48416404 Msa0603750:exon
TCCAGTTGCATTGACAATAG+AGG 0.563787 4_2:+48416166 Msa0603750:CDS
GCAAAATGGGTCTGATTCGT+CGG 0.564076 4_2:+48416686 Msa0603750:three_prime_UTR
TGCAATAATTGAGAAACTAC+CGG 0.569756 4_2:+48416028 Msa0603750:CDS
TACAGATTGAGTCGAAGTGT+TGG 0.577991 4_2:+48416735 Msa0603750:three_prime_UTR
TGTATCTGCTACTTGTTCAG+AGG 0.584728 4_2:-48414180 None:intergenic
AAGGTATGCTGCTTTGTATG+TGG 0.584948 4_2:+48416250 Msa0603750:CDS
TGGGTGTAGGAACAAAATTG+AGG 0.593531 4_2:+48415609 Msa0603750:intron
TGTACATGGATGGACAAGAA+AGG 0.594997 4_2:+48416231 Msa0603750:CDS
GGGCTTAAACATGTGGCTGA+CGG 0.596336 4_2:+48416805 Msa0603750:three_prime_UTR
AATCCACTGAAGATATGTAA+GGG 0.600170 4_2:+48416444 Msa0603750:three_prime_UTR
ACTTCATACATCTCATGACC+AGG 0.605483 4_2:-48416478 None:intergenic
ATCCACTGAAGATATGTAAG+GGG 0.622846 4_2:+48416445 Msa0603750:three_prime_UTR
TATCAAATTAAACATGAGAG+AGG 0.633734 4_2:-48416596 None:intergenic
AAGTAGCAGATACAACAAGT+TGG 0.636568 4_2:+48414189 Msa0603750:five_prime_UTR
AGACAGAAAGCCATAGATTG+CGG 0.662411 4_2:+48416393 Msa0603750:exon
ACTTAATTGAGGAACAATCA+GGG 0.662414 4_2:+48416138 Msa0603750:CDS
TCCACTGAAGATATGTAAGG+GGG 0.683717 4_2:+48416446 Msa0603750:three_prime_UTR
TGTATGTGGAGAAGTCTAGT+CGG 0.687076 4_2:+48416264 Msa0603750:CDS
ATACATGAAACTGAGAACAG+AGG 0.729964 4_2:+48416091 Msa0603750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATCTTCTATTTAAATTA+TGG - chr4_2:48414802-48414821 None:intergenic 15.0%
!! ATTAATAAAAGAGACATTTA+TGG - chr4_2:48415182-48415201 None:intergenic 15.0%
!! AAATGAAATTCTTGAAAATG+TGG + chr4_2:48414668-48414687 Msa0603750:intron 20.0%
!!! ATCATCTAAATGATCATTTT+GGG - chr4_2:48414730-48414749 None:intergenic 20.0%
!!! TATCATCTAAATGATCATTT+TGG - chr4_2:48414731-48414750 None:intergenic 20.0%
!!! TTTTGCGTTGTTATTTTATT+TGG + chr4_2:48414835-48414854 Msa0603750:intron 20.0%
!!! TTTGCGTTGTTATTTTATTT+GGG + chr4_2:48414836-48414855 Msa0603750:intron 20.0%
!!! TATAATTTTCAAACAAAGAG+AGG - chr4_2:48414937-48414956 None:intergenic 20.0%
!!! GTCTCTTTTATTAATCATTT+CGG + chr4_2:48415187-48415206 Msa0603750:intron 20.0%
!! TCAAATCACTATTTAGATTT+AGG + chr4_2:48415213-48415232 Msa0603750:intron 20.0%
!!! ACTATTTAGATTTAGGATAA+TGG + chr4_2:48415220-48415239 Msa0603750:intron 20.0%
!!! CTATTTAGATTTAGGATAAT+GGG + chr4_2:48415221-48415240 Msa0603750:intron 20.0%
!! AATTAATCATTAGCAATCAT+TGG - chr4_2:48415304-48415323 None:intergenic 20.0%
!! CATAAACTAATTCATATGTT+AGG + chr4_2:48415384-48415403 Msa0603750:intron 20.0%
!! AAATATTTATTATTGGGTGT+AGG + chr4_2:48415596-48415615 Msa0603750:intron 20.0%
!! AAAACTGTAAGAAGTTAAAA+AGG - chr4_2:48416332-48416351 None:intergenic 20.0%
!!! GCTGTTTTAATAAGCTATAT+TGG - chr4_2:48414472-48414491 None:intergenic 25.0%
! TATAAGTTGTTGATCAAGAA+CGG + chr4_2:48414595-48414614 Msa0603750:intron 25.0%
!! ATCGATGATAATGTTTATGT+TGG + chr4_2:48414645-48414664 Msa0603750:intron 25.0%
! GGTTAATACTGAAAATATAG+AGG + chr4_2:48414689-48414708 Msa0603750:intron 25.0%
!!! TTGCGTTGTTATTTTATTTG+GGG + chr4_2:48414837-48414856 Msa0603750:intron 25.0%
! GGTGTAAATTTAAAAGTTGA+GGG + chr4_2:48415242-48415261 Msa0603750:intron 25.0%
! GTGTAAATTTAAAAGTTGAG+GGG + chr4_2:48415243-48415262 Msa0603750:intron 25.0%
! TGTAAATTTAAAAGTTGAGG+GGG + chr4_2:48415244-48415263 Msa0603750:intron 25.0%
!!! TTTATGTTAAAAAGTATCCC+AGG - chr4_2:48415370-48415389 None:intergenic 25.0%
!!! TTTTAAGTTTGATTAGTCTG+TGG + chr4_2:48415446-48415465 Msa0603750:intron 25.0%
!!! GAAACATTTTTTTCAAAAGC+AGG - chr4_2:48415735-48415754 None:intergenic 25.0%
!! AAAAAAATGTTTCTTGCTGT+GGG + chr4_2:48415742-48415761 Msa0603750:intron 25.0%
!! CAATATTGATGACTTAATTG+AGG + chr4_2:48416127-48416146 Msa0603750:CDS 25.0%
! ATTTATCGATGAATTGTACA+TGG + chr4_2:48416217-48416236 Msa0603750:CDS 25.0%
! TATCAAATTAAACATGAGAG+AGG - chr4_2:48416599-48416618 None:intergenic 25.0%
! TAAAAAATAGAGGACTAAAC+GGG - chr4_2:48416717-48416736 None:intergenic 25.0%
! GTAAAAAATAGAGGACTAAA+CGG - chr4_2:48416718-48416737 None:intergenic 25.0%
! ACTCAATCTGTAAAAAATAG+AGG - chr4_2:48416727-48416746 None:intergenic 25.0%
! AAAATGAGCTGAATCAATTT+AGG - chr4_2:48416770-48416789 None:intergenic 25.0%
ATTCACAACAAACATAGATC+AGG + chr4_2:48414355-48414374 Msa0603750:intron 30.0%
CATAGATCAGGAAAGTTAAA+CGG + chr4_2:48414367-48414386 Msa0603750:intron 30.0%
ACATAAGTTGTCAATAGCAT+AGG - chr4_2:48414519-48414538 None:intergenic 30.0%
GCTATTGACAACTTATGTTT+AGG + chr4_2:48414521-48414540 Msa0603750:intron 30.0%
TGATCAACAACTTATAGTCA+GGG - chr4_2:48414591-48414610 None:intergenic 30.0%
TTGATCAACAACTTATAGTC+AGG - chr4_2:48414592-48414611 None:intergenic 30.0%
!! TGTTGATGATATTGAAGATG+TGG + chr4_2:48414773-48414792 Msa0603750:intron 30.0%
!! GGCATATTTTTACTTAGAGT+CGG + chr4_2:48414858-48414877 Msa0603750:intron 30.0%
!! GCATATTTTTACTTAGAGTC+GGG + chr4_2:48414859-48414878 Msa0603750:intron 30.0%
GGGTGTAAATTTAAAAGTTG+AGG + chr4_2:48415241-48415260 Msa0603750:intron 30.0%
! CTTGTGGATGATTATTGATT+AGG + chr4_2:48415474-48415493 Msa0603750:intron 30.0%
! GGATGATTATTGATTAGGAT+TGG + chr4_2:48415479-48415498 Msa0603750:intron 30.0%
CTGATTTATGCACAATCTAT+TGG - chr4_2:48415568-48415587 None:intergenic 30.0%
GAAAAAAATGTTTCTTGCTG+TGG + chr4_2:48415741-48415760 Msa0603750:intron 30.0%
AACTTGAATTTGGCTGTAAA+TGG + chr4_2:48415815-48415834 Msa0603750:intron 30.0%
!!! TGCTATTTTGAAAGAGAAGA+CGG + chr4_2:48416004-48416023 Msa0603750:CDS 30.0%
TGCAATAATTGAGAAACTAC+CGG + chr4_2:48416028-48416047 Msa0603750:CDS 30.0%
TTACTGACCTCTTTACATAA+TGG - chr4_2:48416068-48416087 None:intergenic 30.0%
ACTTAATTGAGGAACAATCA+GGG + chr4_2:48416138-48416157 Msa0603750:CDS 30.0%
AATCCACTGAAGATATGTAA+GGG + chr4_2:48416444-48416463 Msa0603750:three_prime_UTR 30.0%
ACAAGAACATATCCTTTAGA+AGG + chr4_2:48416529-48416548 Msa0603750:three_prime_UTR 30.0%
CTCAGACATTGTTTAAAAAG+AGG + chr4_2:48416556-48416575 Msa0603750:three_prime_UTR 30.0%
!!! GCTCATTTTGTCTTGTTTTT+TGG + chr4_2:48416781-48416800 Msa0603750:three_prime_UTR 30.0%
!!! CATTTTGTCTTGTTTTTTGG+CGG + chr4_2:48416784-48416803 Msa0603750:three_prime_UTR 30.0%
!!! ATTTTGTCTTGTTTTTTGGC+GGG + chr4_2:48416785-48416804 Msa0603750:three_prime_UTR 30.0%
!!! GCTACTAGGTTTTGTTGTTA+GGG - chr4_2:48414154-48414173 None:intergenic 35.0%
!!! AGCTACTAGGTTTTGTTGTT+AGG - chr4_2:48414155-48414174 None:intergenic 35.0%
AAGTAGCAGATACAACAAGT+TGG + chr4_2:48414189-48414208 Msa0603750:five_prime_UTR 35.0%
GAAAATCATCATCATCATCC+AGG + chr4_2:48414220-48414239 Msa0603750:five_prime_UTR 35.0%
GTCAATAGCATAGGTTCTAT+GGG - chr4_2:48414510-48414529 None:intergenic 35.0%
TGTCAATAGCATAGGTTCTA+TGG - chr4_2:48414511-48414530 None:intergenic 35.0%
TCCATAAATCAGTATGACAG+CGG - chr4_2:48415127-48415146 None:intergenic 35.0%
AGTGACTACTTCAATACAGA+GGG - chr4_2:48415327-48415346 None:intergenic 35.0%
AAGTGACTACTTCAATACAG+AGG - chr4_2:48415328-48415347 None:intergenic 35.0%
! TGTTAGGTCACTTGTTGAAT+AGG + chr4_2:48415400-48415419 Msa0603750:intron 35.0%
! TGATATCCAATAGTGTTGCT+AGG + chr4_2:48415527-48415546 Msa0603750:intron 35.0%
TGTAGAAGAGAATAAGCAAC+CGG + chr4_2:48415790-48415809 Msa0603750:intron 35.0%
! ACAGCCAAATTCAAGTTTAC+CGG - chr4_2:48415812-48415831 None:intergenic 35.0%
TGAATTTGGCTGTAAATGGA+TGG + chr4_2:48415819-48415838 Msa0603750:intron 35.0%
GAATTTGGCTGTAAATGGAT+GGG + chr4_2:48415820-48415839 Msa0603750:intron 35.0%
!! CACAAACAGTTTCTAGTAAG+CGG - chr4_2:48415864-48415883 None:intergenic 35.0%
AGACATATCCTCTTCCAATT+CGG - chr4_2:48415916-48415935 None:intergenic 35.0%
TCTTCAGGATTCATTGAGAA+AGG + chr4_2:48415935-48415954 Msa0603750:CDS 35.0%
GTGAACCTCATTCTCAAATA+GGG - chr4_2:48415982-48416001 None:intergenic 35.0%
ATACATGAAACTGAGAACAG+AGG + chr4_2:48416091-48416110 Msa0603750:CDS 35.0%
GACTTAATTGAGGAACAATC+AGG + chr4_2:48416137-48416156 Msa0603750:CDS 35.0%
ATCGATGAATTGTACATGGA+TGG + chr4_2:48416221-48416240 Msa0603750:CDS 35.0%
! ACAGTTTTTCTTGTCACTGT+AGG + chr4_2:48416344-48416363 Msa0603750:intron 35.0%
CAATCCACTGAAGATATGTA+AGG + chr4_2:48416443-48416462 Msa0603750:three_prime_UTR 35.0%
ATCCACTGAAGATATGTAAG+GGG + chr4_2:48416445-48416464 Msa0603750:three_prime_UTR 35.0%
AAATATGCATGCAGAGATAG+AGG + chr4_2:48416661-48416680 Msa0603750:three_prime_UTR 35.0%
AGAGATAGAGGTAGCAAAAT+GGG + chr4_2:48416673-48416692 Msa0603750:three_prime_UTR 35.0%
! TGTATCTGCTACTTGTTCAG+AGG - chr4_2:48414183-48414202 None:intergenic 40.0%
CGAACAGAAAAAGAGAGAAG+GGG - chr4_2:48414253-48414272 None:intergenic 40.0%
CCGAACAGAAAAAGAGAGAA+GGG - chr4_2:48414254-48414273 None:intergenic 40.0%
! CCCTTCTCTCTTTTTCTGTT+CGG + chr4_2:48414251-48414270 Msa0603750:intron 40.0%
TCCGAACAGAAAAAGAGAGA+AGG - chr4_2:48414255-48414274 None:intergenic 40.0%
ACTTATAGTCAGGGTGTGTA+TGG - chr4_2:48414582-48414601 None:intergenic 40.0%
GATCAAGAACGGTCAATTTC+AGG + chr4_2:48414606-48414625 Msa0603750:intron 40.0%
TAGAGTCGTCCCTATCAAAT+TGG - chr4_2:48414888-48414907 None:intergenic 40.0%
!! TAGGGACGACTCTATTTTGA+AGG + chr4_2:48414894-48414913 Msa0603750:intron 40.0%
!! TCCGCTGTCATACTGATTTA+TGG + chr4_2:48415123-48415142 Msa0603750:intron 40.0%
TTTATACAAGCCTATGCTCC+TGG + chr4_2:48415349-48415368 Msa0603750:intron 40.0%
TTATACAAGCCTATGCTCCT+GGG + chr4_2:48415350-48415369 Msa0603750:intron 40.0%
!! AAAAAGTATCCCAGGAGCAT+AGG - chr4_2:48415362-48415381 None:intergenic 40.0%
TTAGTCTGTGGATGTTCTTG+TGG + chr4_2:48415458-48415477 Msa0603750:intron 40.0%
CAAAAGCCTAGCAACACTAT+TGG - chr4_2:48415536-48415555 None:intergenic 40.0%
!! CAATAGTGTTGCTAGGCTTT+TGG + chr4_2:48415534-48415553 Msa0603750:intron 40.0%
TGGGTGTAGGAACAAAATTG+AGG + chr4_2:48415609-48415628 Msa0603750:intron 40.0%
GCAACCGGTAAACTTGAATT+TGG + chr4_2:48415805-48415824 Msa0603750:intron 40.0%
CGCTTACTAGAAACTGTTTG+TGG + chr4_2:48415862-48415881 Msa0603750:intron 40.0%
TGAATTGATTGCAGCCGAAT+TGG + chr4_2:48415899-48415918 Msa0603750:CDS 40.0%
AACTGCCCTATTTGAGAATG+AGG + chr4_2:48415974-48415993 Msa0603750:CDS 40.0%
CGTGAACCTCATTCTCAAAT+AGG - chr4_2:48415983-48416002 None:intergenic 40.0%
TGCAGGTCCATTATGTAAAG+AGG + chr4_2:48416058-48416077 Msa0603750:CDS 40.0%
! TCCAGTTGCATTGACAATAG+AGG + chr4_2:48416166-48416185 Msa0603750:CDS 40.0%
ACCTCTATTGTCAATGCAAC+TGG - chr4_2:48416170-48416189 None:intergenic 40.0%
TGTACATGGATGGACAAGAA+AGG + chr4_2:48416231-48416250 Msa0603750:CDS 40.0%
!! AAGGTATGCTGCTTTGTATG+TGG + chr4_2:48416250-48416269 Msa0603750:CDS 40.0%
TGTATGTGGAGAAGTCTAGT+CGG + chr4_2:48416264-48416283 Msa0603750:CDS 40.0%
CTAGTCGGTAAGTTATACAC+TGG + chr4_2:48416279-48416298 Msa0603750:intron 40.0%
AGACAGAAAGCCATAGATTG+CGG + chr4_2:48416393-48416412 Msa0603750:exon 40.0%
CACAATTTGACCGCAATCTA+TGG - chr4_2:48416406-48416425 None:intergenic 40.0%
CATAGATTGCGGTCAAATTG+TGG + chr4_2:48416404-48416423 Msa0603750:exon 40.0%
TCCACTGAAGATATGTAAGG+GGG + chr4_2:48416446-48416465 Msa0603750:three_prime_UTR 40.0%
GTAAGGGGGTAAAAAAAACC+TGG + chr4_2:48416460-48416479 Msa0603750:three_prime_UTR 40.0%
ACTTCATACATCTCATGACC+AGG - chr4_2:48416481-48416500 None:intergenic 40.0%
! TGTCTGAGACAACCTTCTAA+AGG - chr4_2:48416544-48416563 None:intergenic 40.0%
CAGAGATAGAGGTAGCAAAA+TGG + chr4_2:48416672-48416691 Msa0603750:three_prime_UTR 40.0%
TACAGATTGAGTCGAAGTGT+TGG + chr4_2:48416735-48416754 Msa0603750:three_prime_UTR 40.0%
TTCCTTCCCCTTTCTCGATA+CGG + chr4_2:48414277-48414296 Msa0603750:intron 45.0%
ACGGTTGAGTAGACGATGTT+TGG + chr4_2:48414386-48414405 Msa0603750:intron 45.0%
CGGTTGAGTAGACGATGTTT+GGG + chr4_2:48414387-48414406 Msa0603750:intron 45.0%
AGTCGGGTCTCCAATTTGAT+AGG + chr4_2:48414875-48414894 Msa0603750:intron 45.0%
GTCGGGTCTCCAATTTGATA+GGG + chr4_2:48414876-48414895 Msa0603750:intron 45.0%
!! GGACGACTCTATTTTGAAGG+AGG + chr4_2:48414897-48414916 Msa0603750:intron 45.0%
TCAAACAAAGAGAGGCAGCA+AGG - chr4_2:48414929-48414948 None:intergenic 45.0%
GACAGCGGAGTATACAATCA+GGG - chr4_2:48415112-48415131 None:intergenic 45.0%
TGACAGCGGAGTATACAATC+AGG - chr4_2:48415113-48415132 None:intergenic 45.0%
TGAATCCCCTGGTTAGTAGA+TGG + chr4_2:48415273-48415292 Msa0603750:intron 45.0%
CAACATTTCCGAGAAGTCCA+TGG + chr4_2:48415655-48415674 Msa0603750:CDS 45.0%
TTCCGAGAAGTCCATGGAAA+AGG + chr4_2:48415661-48415680 Msa0603750:CDS 45.0%
! CACCTTTTCCATGGACTTCT+CGG - chr4_2:48415666-48415685 None:intergenic 45.0%
! TGTATGTCGCACCTTTTCCA+TGG - chr4_2:48415675-48415694 None:intergenic 45.0%
GGAAGAGGATATGTCTCTTC+AGG + chr4_2:48415920-48415939 Msa0603750:CDS 45.0%
ACCCCCTTACATATCTTCAG+TGG - chr4_2:48416450-48416469 None:intergenic 45.0%
! GCAAAATGGGTCTGATTCGT+CGG + chr4_2:48416686-48416705 Msa0603750:three_prime_UTR 45.0%
!! TTGAGTCGAAGTGTTGGACT+TGG + chr4_2:48416741-48416760 Msa0603750:three_prime_UTR 45.0%
!!! TTTTGGCGGGCTTAAACATG+TGG + chr4_2:48416798-48416817 Msa0603750:three_prime_UTR 45.0%
!! TTATTCTAAATATTTATTAT+TGG + chr4_2:48415589-48415608 Msa0603750:intron 5.0%
!! TATTCTAAATATTTATTATT+GGG + chr4_2:48415590-48415609 Msa0603750:intron 5.0%
GAGAGAAGGGGAAACAAACC+TGG - chr4_2:48414241-48414260 None:intergenic 50.0%
TGAATTGCGCCGCATTGAAG+GGG - chr4_2:48414340-48414359 None:intergenic 50.0%
GTGAATTGCGCCGCATTGAA+GGG - chr4_2:48414341-48414360 None:intergenic 50.0%
TGTGAATTGCGCCGCATTGA+AGG - chr4_2:48414342-48414361 None:intergenic 50.0%
CAGAGCCATCTACTAACCAG+GGG - chr4_2:48415281-48415300 None:intergenic 50.0%
GCAGAGCCATCTACTAACCA+GGG - chr4_2:48415282-48415301 None:intergenic 50.0%
! CTTGCTGTGGGCAAAACTGA+AGG + chr4_2:48415754-48415773 Msa0603750:intron 50.0%
GATTGCAGCCGAATTGGAAG+AGG + chr4_2:48415905-48415924 Msa0603750:CDS 50.0%
! AAACTACCGGAGCATCTTGC+AGG + chr4_2:48416041-48416060 Msa0603750:CDS 50.0%
AATGGACCTGCAAGATGCTC+CGG - chr4_2:48416050-48416069 None:intergenic 50.0%
CATGAGAGAGGACGCGATTT+AGG - chr4_2:48416587-48416606 None:intergenic 50.0%
GGGCTTAAACATGTGGCTGA+CGG + chr4_2:48416805-48416824 Msa0603750:three_prime_UTR 50.0%
! TTCAGAGGCGCAGAGCTACT+AGG - chr4_2:48414168-48414187 None:intergenic 55.0%
CTTCCCCTTTCTCGATACGG+CGG + chr4_2:48414280-48414299 Msa0603750:intron 55.0%
GGCAGAGCCATCTACTAACC+AGG - chr4_2:48415283-48415302 None:intergenic 55.0%
CGCCGTATCGAGAAAGGGGA+AGG - chr4_2:48414282-48414301 None:intergenic 60.0%
TGCGCCGCCGTATCGAGAAA+GGG - chr4_2:48414287-48414306 None:intergenic 60.0%
CCGCATTGAAGGGGCGGATA+CGG - chr4_2:48414331-48414350 None:intergenic 60.0%
CCGTATCCGCCCCTTCAATG+CGG + chr4_2:48414328-48414347 Msa0603750:intron 60.0%
ATTGCGCCGCATTGAAGGGG+CGG - chr4_2:48414337-48414356 None:intergenic 60.0%
GCGCCGCCGTATCGAGAAAG+GGG - chr4_2:48414286-48414305 None:intergenic 65.0%
GTGCGCCGCCGTATCGAGAA+AGG - chr4_2:48414288-48414307 None:intergenic 65.0%
GCGCACGAGTATGCCATCGC+TGG + chr4_2:48414302-48414321 Msa0603750:intron 65.0%
GGGGGTTCGACTGAATCCCC+TGG + chr4_2:48415262-48415281 Msa0603750:intron 65.0%
GCGGATACGGCAGCCAGCGA+TGG - chr4_2:48414318-48414337 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 48414136 48416844 48414136 ID=Msa0603750;Name=Msa0603750
chr4_2 mRNA 48414136 48416844 48414136 ID=Msa0603750-mRNA-1;Parent=Msa0603750;Name=Msa0603750-mRNA-1;_AED=0.06;_eAED=0.06;_QI=135|1|1|1|0.66|0.5|4|436|157
chr4_2 exon 48414136 48414241 48414136 ID=Msa0603750-mRNA-1:exon:11163;Parent=Msa0603750-mRNA-1
chr4_2 exon 48415618 48415682 48415618 ID=Msa0603750-mRNA-1:exon:11164;Parent=Msa0603750-mRNA-1
chr4_2 exon 48415891 48416285 48415891 ID=Msa0603750-mRNA-1:exon:11165;Parent=Msa0603750-mRNA-1
chr4_2 exon 48416366 48416844 48416366 ID=Msa0603750-mRNA-1:exon:11166;Parent=Msa0603750-mRNA-1
chr4_2 five_prime_UTR 48414136 48414241 48414136 ID=Msa0603750-mRNA-1:five_prime_utr;Parent=Msa0603750-mRNA-1
chr4_2 five_prime_UTR 48415618 48415646 48415618 ID=Msa0603750-mRNA-1:five_prime_utr;Parent=Msa0603750-mRNA-1
chr4_2 CDS 48415647 48415682 48415647 ID=Msa0603750-mRNA-1:cds;Parent=Msa0603750-mRNA-1
chr4_2 CDS 48415891 48416285 48415891 ID=Msa0603750-mRNA-1:cds;Parent=Msa0603750-mRNA-1
chr4_2 CDS 48416366 48416408 48416366 ID=Msa0603750-mRNA-1:cds;Parent=Msa0603750-mRNA-1
chr4_2 three_prime_UTR 48416409 48416844 48416409 ID=Msa0603750-mRNA-1:three_prime_utr;Parent=Msa0603750-mRNA-1
Gene Sequence

>Msa0603750

ATGAAGTTCAACATTTCCGAGAAGTCCATGGAAAAGCAAATTGATGAATTGATTGCAGCCGAATTGGAAGAGGATATGTCTCTTCAGGATTCATTGAGAAAGGTTAATGACTTGTTTATAACTGCCCTATTTGAGAATGAGGTTCACGCTGCTATTTTGAAAGAGAAGACGGATGCAATAATTGAGAAACTACCGGAGCATCTTGCAGGTCCATTATGTAAAGAGGTCAGTAAAATATACATGAAACTGAGAACAGAGGTCAGTGAATTCTTCAATATTGATGACTTAATTGAGGAACAATCAGGGCGTGTTCCAGTTGCATTGACAATAGAGGTCATGAAAACGAACATGAAATTGCGAAAATTTATCGATGAATTGTACATGGATGGACAAGAAAGGTATGCTGCTTTGTATGTGGAGAAGTCTAGTCGGTTAATCAAAGACAAAACAGAAAAGCAAGACAGAAAGCCATAG
Protein sequence

>Msa0603750

MKFNISEKSMEKQIDELIAAELEEDMSLQDSLRKVNDLFITALFENEVHAAILKEKTDAIIEKLPEHLAGPLCKEVSKIYMKLRTEVSEFFNIDDLIEEQSGRVPVALTIEVMKTNMKLRKFIDELYMDGQERYAALYVEKSSRLIKDKTEKQDRKP*