AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0115730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115730 MtrunA17_Chr1g0212121 93.578 218 14 0 1 218 1 218 2.18e-153 424
Msa0115730 MtrunA17_Chr1g0212181 86.697 218 29 0 1 218 1 218 1.77e-141 394
Msa0115730 MtrunA17_Chr1g0212171 76.923 221 48 1 1 218 1 221 7.09e-126 355
Msa0115730 MtrunA17_Chr1g0212091 54.825 228 84 3 1 217 1 220 5.53e-81 241
Msa0115730 MtrunA17_Chr1g0162171 55.310 226 83 3 5 218 4 223 5.48e-80 238
Msa0115730 MtrunA17_Chr8g0388871 48.889 180 91 1 40 218 6 185 2.78e-55 174
Msa0115730 MtrunA17_Chr6g0481891 44.792 192 101 1 27 218 13 199 1.50e-52 168
Msa0115730 MtrunA17_Chr1g0212111 63.964 111 40 0 108 218 47 157 2.99e-43 143
Msa0115730 MtrunA17_Chr8g0367701 36.478 159 100 1 57 215 110 267 3.43e-37 130
Msa0115730 MtrunA17_Chr3g0133221 36.364 154 97 1 62 215 103 255 1.82e-36 128
Msa0115730 MtrunA17_Chr1g0212131 85.938 64 9 0 155 218 1 64 8.94e-35 118
Msa0115730 MtrunA17_Chr7g0251801 35.583 163 105 0 55 217 114 276 2.64e-33 121
Msa0115730 MtrunA17_Chr2g0315771 32.967 182 119 2 39 217 46 227 4.56e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115730 AT5G23370.1 49.083 218 105 3 1 216 1 214 3.74e-70 214
Msa0115730 AT5G08350.1 51.220 205 91 2 12 216 18 213 1.29e-67 207
Msa0115730 AT5G23350.1 47.945 219 104 4 1 215 1 213 1.69e-66 204
Msa0115730 AT5G23360.1 44.954 218 108 3 1 217 1 207 2.31e-62 193
Msa0115730 AT4G01600.3 38.750 160 97 1 57 215 26 185 1.09e-37 130
Msa0115730 AT5G13200.1 37.654 162 101 0 55 216 100 261 1.56e-37 132
Msa0115730 AT4G01600.1 38.750 160 97 1 57 215 65 224 3.36e-37 130
Msa0115730 AT4G01600.2 34.500 200 116 4 31 215 20 219 1.10e-35 126
Msa0115730 AT2G22475.1 34.375 160 104 1 56 215 133 291 1.86e-34 124
Msa0115730 AT1G28200.1 34.591 159 103 1 57 215 97 254 1.28e-33 121
Msa0115730 AT1G28200.2 34.591 159 103 1 57 215 97 254 1.28e-33 121
Msa0115730 AT5G13200.2 36.697 109 69 0 55 163 100 208 2.03e-21 88.6
Msa0115730 AT2G22475.2 32.456 114 76 1 56 169 133 245 6.23e-17 77.4

Find 55 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTTTGATTTCTGGCTTA+TGG 0.171332 1_3:-34677826 Msa0115730:CDS
CAGTGGACAACTTTGATTTC+TGG 0.206063 1_3:-34677833 Msa0115730:CDS
AACTTTGATTTCTGGCTTAT+GGG 0.269568 1_3:-34677825 Msa0115730:CDS
AAACATTATAGTTGCAGAAT+TGG 0.280963 1_3:-34677636 Msa0115730:three_prime_UTR
AAGGTTACTCACCATGTTCT+TGG 0.292240 1_3:+34678466 None:intergenic
TTAATCAACAATATGTTGAT+AGG 0.341754 1_3:+34677489 None:intergenic
ATACCATCAGTTTCAAAATT+CGG 0.341905 1_3:-34678699 Msa0115730:CDS
CTTCTTCATGAGCTAGTTGT+TGG 0.342165 1_3:-34678734 Msa0115730:CDS
CTTTGATAAGAAGGACTTCA+TGG 0.357671 1_3:-34677606 Msa0115730:three_prime_UTR
TAATTCGGAGCACATGGTTA+TGG 0.366438 1_3:-34677540 Msa0115730:three_prime_UTR
TAAAGAGAAAATTGAGCTTA+GGG 0.381007 1_3:-34678264 Msa0115730:CDS
TATTGATCTGTCACTGCAAA+AGG 0.396030 1_3:+34678087 None:intergenic
AACATTATAGTTGCAGAATT+GGG 0.397100 1_3:-34677635 Msa0115730:three_prime_UTR
ATAAAGAGAAAATTGAGCTT+AGG 0.408838 1_3:-34678265 Msa0115730:CDS
ACACACTTGATATTCTTTAG+TGG 0.408894 1_3:+34677909 None:intergenic
TAACAAATTCGAACAAGAAA+AGG 0.412169 1_3:-34678524 Msa0115730:CDS
TGAAACTGATGGTATATGAT+TGG 0.417569 1_3:+34678707 None:intergenic
AATGCGTAGGATCCGCTATA+AGG 0.432925 1_3:-34678041 Msa0115730:intron
CAAGAAAAGGCTTGCTAGGA+AGG 0.445665 1_3:-34678511 Msa0115730:CDS
TGTTATCTATCCACCACATC+TGG 0.458472 1_3:-34678151 Msa0115730:CDS
CTTGATATTCTTTAGTGGAA+TGG 0.461377 1_3:+34677914 None:intergenic
GGGCACTGTCTTTGATAAGA+AGG 0.475678 1_3:-34677615 Msa0115730:three_prime_UTR
ACCACATCTGGCCCTCTAGC+TGG 0.475765 1_3:-34678139 Msa0115730:CDS
CGCATGCTTTGTTACATGAA+AGG 0.488384 1_3:-34677442 Msa0115730:three_prime_UTR
AACTTTCAAATATCTTGAGC+AGG 0.496933 1_3:-34677784 Msa0115730:CDS
CGAACAAGAAAAGGCTTGCT+AGG 0.498890 1_3:-34678515 Msa0115730:CDS
ACTAGCAGTAGAAGTTGAAG+AGG 0.506600 1_3:+34678810 None:intergenic
ATATTTATGTACTTCTGCGT+TGG 0.511134 1_3:+34677861 None:intergenic
ACATATTGTTGATTAAATTG+TGG 0.518758 1_3:-34677482 Msa0115730:three_prime_UTR
CCTTCTCGTTCGAGATGAAG+AGG 0.537454 1_3:+34678113 None:intergenic
ATAAAAGTCTTTAATCAGAA+AGG 0.537723 1_3:-34678067 Msa0115730:CDS
AGCTAGAGGGCCAGATGTGG+TGG 0.540629 1_3:+34678141 None:intergenic
ATGAAGAGGAGACCAGCTAG+AGG 0.548150 1_3:+34678127 None:intergenic
ATGGTAGAAAGCAAGATGGA+AGG 0.559002 1_3:-34677408 Msa0115730:three_prime_UTR
ATAATATAAATAGTCGAGGC+TGG 0.562226 1_3:-34677716 Msa0115730:three_prime_UTR
AGGGGCTCAAATTCTTCAAG+TGG 0.580559 1_3:-34678245 Msa0115730:CDS
GAATGAAGCTTCACGTTGCG+AGG 0.581949 1_3:-34677694 Msa0115730:three_prime_UTR
AATTCTTCAAGTGGGTGGAG+TGG 0.586812 1_3:-34678236 Msa0115730:CDS
GGTTATGGTTCATTTCAGCT+CGG 0.591369 1_3:-34677525 Msa0115730:three_prime_UTR
CCTCTTCATCTCGAACGAGA+AGG 0.596051 1_3:-34678113 Msa0115730:CDS
ATCTGGGACTTACCTTATAG+CGG 0.601651 1_3:+34678029 None:intergenic
CTATTTATATTATGCACATG+TGG 0.606244 1_3:+34677726 None:intergenic
ATCAGAAAGGTCAAATGCGT+AGG 0.606952 1_3:-34678054 Msa0115730:CDS
GCTCAAATTCTTCAAGTGGG+TGG 0.609214 1_3:-34678241 Msa0115730:CDS
GGGGCTCAAATTCTTCAAGT+GGG 0.611003 1_3:-34678244 Msa0115730:CDS
ATGCTTTGTTACATGAAAGG+AGG 0.618476 1_3:-34677439 Msa0115730:three_prime_UTR
TTTATATTATGCACATGTGG+CGG 0.623871 1_3:+34677729 None:intergenic
GGAATGTTCTGAGCTACAAG+AGG 0.629624 1_3:+34678831 None:intergenic
ACCAGCTAGAGGGCCAGATG+TGG 0.630645 1_3:+34678138 None:intergenic
TGAAGAGGAGACCAGCTAGA+GGG 0.639442 1_3:+34678128 None:intergenic
AACTAGCTCATGAAGAAGTG+AGG 0.642281 1_3:+34678739 None:intergenic
GTACATAAATATAGTTACAG+TGG 0.643140 1_3:-34677850 Msa0115730:CDS
GTGCATAATATAAATAGTCG+AGG 0.643295 1_3:-34677720 Msa0115730:three_prime_UTR
AAAGAGAAAATTGAGCTTAG+GGG 0.647324 1_3:-34678263 Msa0115730:CDS
GAGTGGAGAAAGTGTTTATG+CGG 0.656181 1_3:-34678219 Msa0115730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTTAAAATTCTTAA+TGG - chr1_3:34678250-34678269 Msa0115730:CDS 10.0%
!!! AAGATTTTTAAAATTCTTAA+TGG - chr1_3:34678250-34678269 Msa0115730:CDS 10.0%
!! ATAAAGATATTGCAAACAAA+AGG + chr1_3:34677825-34677844 None:intergenic 20.0%
!! ATAAAAGTCTTTAATCAGAA+AGG - chr1_3:34678146-34678165 Msa0115730:CDS 20.0%
!! TTAATCAACAATATGTTGAT+AGG + chr1_3:34678727-34678746 None:intergenic 20.0%
!!! ACATATTGTTGATTAAATTG+TGG - chr1_3:34678731-34678750 Msa0115730:CDS 20.0%
!! ATAAAGATATTGCAAACAAA+AGG + chr1_3:34677825-34677844 None:intergenic 20.0%
!! ATAAAAGTCTTTAATCAGAA+AGG - chr1_3:34678146-34678165 Msa0115730:CDS 20.0%
!! TTAATCAACAATATGTTGAT+AGG + chr1_3:34678727-34678746 None:intergenic 20.0%
!!! ACATATTGTTGATTAAATTG+TGG - chr1_3:34678731-34678750 Msa0115730:CDS 20.0%
! ATACCATCAGTTTCAAAATT+CGG - chr1_3:34677514-34677533 Msa0115730:three_prime_UTR 25.0%
! TAACAAATTCGAACAAGAAA+AGG - chr1_3:34677689-34677708 Msa0115730:three_prime_UTR 25.0%
! ATAAACGATGCAATAACAAT+TGG + chr1_3:34677792-34677811 None:intergenic 25.0%
! ATAAAGAGAAAATTGAGCTT+AGG - chr1_3:34677948-34677967 Msa0115730:intron 25.0%
! TAAAGAGAAAATTGAGCTTA+GGG - chr1_3:34677949-34677968 Msa0115730:intron 25.0%
! AGGTTTGTGTATATTAATCT+GGG + chr1_3:34678203-34678222 None:intergenic 25.0%
! TAGGTTTGTGTATATTAATC+TGG + chr1_3:34678204-34678223 None:intergenic 25.0%
! GTACATAAATATAGTTACAG+TGG - chr1_3:34678363-34678382 Msa0115730:intron 25.0%
! CTATTTATATTATGCACATG+TGG + chr1_3:34678490-34678509 None:intergenic 25.0%
! AAACATTATAGTTGCAGAAT+TGG - chr1_3:34678577-34678596 Msa0115730:intron 25.0%
! AACATTATAGTTGCAGAATT+GGG - chr1_3:34678578-34678597 Msa0115730:intron 25.0%
!!! CATCTAGCTGAATTTTAATT+CGG - chr1_3:34678658-34678677 Msa0115730:CDS 25.0%
!!! ATGAAAGGAGGTTATTTTTA+TGG - chr1_3:34678786-34678805 Msa0115730:five_prime_UTR 25.0%
! ATACCATCAGTTTCAAAATT+CGG - chr1_3:34677514-34677533 Msa0115730:three_prime_UTR 25.0%
! TAACAAATTCGAACAAGAAA+AGG - chr1_3:34677689-34677708 Msa0115730:three_prime_UTR 25.0%
! ATAAACGATGCAATAACAAT+TGG + chr1_3:34677792-34677811 None:intergenic 25.0%
! ATAAAGAGAAAATTGAGCTT+AGG - chr1_3:34677948-34677967 Msa0115730:intron 25.0%
! TAAAGAGAAAATTGAGCTTA+GGG - chr1_3:34677949-34677968 Msa0115730:intron 25.0%
! AGGTTTGTGTATATTAATCT+GGG + chr1_3:34678203-34678222 None:intergenic 25.0%
! TAGGTTTGTGTATATTAATC+TGG + chr1_3:34678204-34678223 None:intergenic 25.0%
! GTACATAAATATAGTTACAG+TGG - chr1_3:34678363-34678382 Msa0115730:intron 25.0%
! CTATTTATATTATGCACATG+TGG + chr1_3:34678490-34678509 None:intergenic 25.0%
! AAACATTATAGTTGCAGAAT+TGG - chr1_3:34678577-34678596 Msa0115730:intron 25.0%
! AACATTATAGTTGCAGAATT+GGG - chr1_3:34678578-34678597 Msa0115730:intron 25.0%
!!! CATCTAGCTGAATTTTAATT+CGG - chr1_3:34678658-34678677 Msa0115730:CDS 25.0%
!!! ATGAAAGGAGGTTATTTTTA+TGG - chr1_3:34678786-34678805 Msa0115730:five_prime_UTR 25.0%
TGAAACTGATGGTATATGAT+TGG + chr1_3:34677509-34677528 None:intergenic 30.0%
!!! TAACCGAATTTTGAAACTGA+TGG + chr1_3:34677520-34677539 None:intergenic 30.0%
!! CTTACTTTTGCTTTGATGTT+TGG + chr1_3:34677588-34677607 None:intergenic 30.0%
ATCATGAACATCTGTATTGA+TGG - chr1_3:34677631-34677650 Msa0115730:three_prime_UTR 30.0%
! AACGATAAAGCACTCAATAT+TGG + chr1_3:34677662-34677681 None:intergenic 30.0%
ATCAAGTAAATGACTGTACA+AGG + chr1_3:34677861-34677880 None:intergenic 30.0%
AAAGAGAAAATTGAGCTTAG+GGG - chr1_3:34677950-34677969 Msa0115730:intron 30.0%
CTTGATATTCTTTAGTGGAA+TGG + chr1_3:34678302-34678321 None:intergenic 30.0%
ACACACTTGATATTCTTTAG+TGG + chr1_3:34678307-34678326 None:intergenic 30.0%
ATATTTATGTACTTCTGCGT+TGG + chr1_3:34678355-34678374 None:intergenic 30.0%
!! AACTTTGATTTCTGGCTTAT+GGG - chr1_3:34678388-34678407 Msa0115730:intron 30.0%
AACTTTCAAATATCTTGAGC+AGG - chr1_3:34678429-34678448 Msa0115730:intron 30.0%
TTTATATTATGCACATGTGG+CGG + chr1_3:34678487-34678506 None:intergenic 30.0%
GTGCATAATATAAATAGTCG+AGG - chr1_3:34678493-34678512 Msa0115730:CDS 30.0%
ATAATATAAATAGTCGAGGC+TGG - chr1_3:34678497-34678516 Msa0115730:CDS 30.0%
! TTTTATGGTAGAAAGCAAGA+TGG - chr1_3:34678801-34678820 Msa0115730:five_prime_UTR 30.0%
TGAAACTGATGGTATATGAT+TGG + chr1_3:34677509-34677528 None:intergenic 30.0%
!!! TAACCGAATTTTGAAACTGA+TGG + chr1_3:34677520-34677539 None:intergenic 30.0%
!! CTTACTTTTGCTTTGATGTT+TGG + chr1_3:34677588-34677607 None:intergenic 30.0%
ATCATGAACATCTGTATTGA+TGG - chr1_3:34677631-34677650 Msa0115730:three_prime_UTR 30.0%
! AACGATAAAGCACTCAATAT+TGG + chr1_3:34677662-34677681 None:intergenic 30.0%
ATCAAGTAAATGACTGTACA+AGG + chr1_3:34677861-34677880 None:intergenic 30.0%
AAAGAGAAAATTGAGCTTAG+GGG - chr1_3:34677950-34677969 Msa0115730:intron 30.0%
CTTGATATTCTTTAGTGGAA+TGG + chr1_3:34678302-34678321 None:intergenic 30.0%
ACACACTTGATATTCTTTAG+TGG + chr1_3:34678307-34678326 None:intergenic 30.0%
ATATTTATGTACTTCTGCGT+TGG + chr1_3:34678355-34678374 None:intergenic 30.0%
!! AACTTTGATTTCTGGCTTAT+GGG - chr1_3:34678388-34678407 Msa0115730:intron 30.0%
AACTTTCAAATATCTTGAGC+AGG - chr1_3:34678429-34678448 Msa0115730:intron 30.0%
TTTATATTATGCACATGTGG+CGG + chr1_3:34678487-34678506 None:intergenic 30.0%
GTGCATAATATAAATAGTCG+AGG - chr1_3:34678493-34678512 Msa0115730:CDS 30.0%
ATAATATAAATAGTCGAGGC+TGG - chr1_3:34678497-34678516 Msa0115730:CDS 30.0%
! TTTTATGGTAGAAAGCAAGA+TGG - chr1_3:34678801-34678820 Msa0115730:five_prime_UTR 30.0%
! CTTTTGCTTTGATGTTTGGA+TGG + chr1_3:34677584-34677603 None:intergenic 35.0%
TGATTCGTCTGTAAAGGAAA+TGG + chr1_3:34677615-34677634 None:intergenic 35.0%
TGTTCATGATTCGTCTGTAA+AGG + chr1_3:34677621-34677640 None:intergenic 35.0%
ATTGAGTGCTTTATCGTTTC+AGG - chr1_3:34677664-34677683 Msa0115730:three_prime_UTR 35.0%
TCACAAAAAGTCCAAGAACA+TGG - chr1_3:34677736-34677755 Msa0115730:three_prime_UTR 35.0%
AAACACATCTGAGTCTTACA+AGG + chr1_3:34677769-34677788 None:intergenic 35.0%
GATATTGCAAACAAAAGGCA+TGG + chr1_3:34677820-34677839 None:intergenic 35.0%
TATTGATCTGTCACTGCAAA+AGG + chr1_3:34678129-34678148 None:intergenic 35.0%
ACAAACCTATTCATATCTCG+CGG - chr1_3:34678215-34678234 Msa0115730:CDS 35.0%
AAAAACCGCGAGATATGAAT+AGG + chr1_3:34678223-34678242 None:intergenic 35.0%
TATGTATATCTCTCATGCAC+AGG - chr1_3:34678273-34678292 Msa0115730:CDS 35.0%
!! CAACTTTGATTTCTGGCTTA+TGG - chr1_3:34678387-34678406 Msa0115730:intron 35.0%
CTTTGATAAGAAGGACTTCA+TGG - chr1_3:34678607-34678626 Msa0115730:intron 35.0%
! AGCTAGATGTTTTGTGCTTT+AGG + chr1_3:34678647-34678666 None:intergenic 35.0%
!!! GAATTTTAATTCGGAGCACA+TGG - chr1_3:34678667-34678686 Msa0115730:CDS 35.0%
! ATGCTTTGTTACATGAAAGG+AGG - chr1_3:34678774-34678793 Msa0115730:five_prime_UTR 35.0%
! CTTTTGCTTTGATGTTTGGA+TGG + chr1_3:34677584-34677603 None:intergenic 35.0%
TGATTCGTCTGTAAAGGAAA+TGG + chr1_3:34677615-34677634 None:intergenic 35.0%
TGTTCATGATTCGTCTGTAA+AGG + chr1_3:34677621-34677640 None:intergenic 35.0%
ATTGAGTGCTTTATCGTTTC+AGG - chr1_3:34677664-34677683 Msa0115730:three_prime_UTR 35.0%
TCACAAAAAGTCCAAGAACA+TGG - chr1_3:34677736-34677755 Msa0115730:three_prime_UTR 35.0%
AAACACATCTGAGTCTTACA+AGG + chr1_3:34677769-34677788 None:intergenic 35.0%
GATATTGCAAACAAAAGGCA+TGG + chr1_3:34677820-34677839 None:intergenic 35.0%
TATTGATCTGTCACTGCAAA+AGG + chr1_3:34678129-34678148 None:intergenic 35.0%
ACAAACCTATTCATATCTCG+CGG - chr1_3:34678215-34678234 Msa0115730:CDS 35.0%
AAAAACCGCGAGATATGAAT+AGG + chr1_3:34678223-34678242 None:intergenic 35.0%
TATGTATATCTCTCATGCAC+AGG - chr1_3:34678273-34678292 Msa0115730:CDS 35.0%
!! CAACTTTGATTTCTGGCTTA+TGG - chr1_3:34678387-34678406 Msa0115730:intron 35.0%
CTTTGATAAGAAGGACTTCA+TGG - chr1_3:34678607-34678626 Msa0115730:intron 35.0%
! AGCTAGATGTTTTGTGCTTT+AGG + chr1_3:34678647-34678666 None:intergenic 35.0%
!!! GAATTTTAATTCGGAGCACA+TGG - chr1_3:34678667-34678686 Msa0115730:CDS 35.0%
! ATGCTTTGTTACATGAAAGG+AGG - chr1_3:34678774-34678793 Msa0115730:five_prime_UTR 35.0%
ACTAGCAGTAGAAGTTGAAG+AGG + chr1_3:34677406-34677425 None:intergenic 40.0%
AACTAGCTCATGAAGAAGTG+AGG + chr1_3:34677477-34677496 None:intergenic 40.0%
CTTCTTCATGAGCTAGTTGT+TGG - chr1_3:34677479-34677498 Msa0115730:three_prime_UTR 40.0%
AAGGTTACTCACCATGTTCT+TGG + chr1_3:34677750-34677769 None:intergenic 40.0%
!! GAGTGGAGAAAGTGTTTATG+CGG - chr1_3:34677994-34678013 Msa0115730:intron 40.0%
TGTTATCTATCCACCACATC+TGG - chr1_3:34678062-34678081 Msa0115730:CDS 40.0%
ATCAGAAAGGTCAAATGCGT+AGG - chr1_3:34678159-34678178 Msa0115730:CDS 40.0%
! ATCTGGGACTTACCTTATAG+CGG + chr1_3:34678187-34678206 None:intergenic 40.0%
! CAGTGGACAACTTTGATTTC+TGG - chr1_3:34678380-34678399 Msa0115730:intron 40.0%
TAATTCGGAGCACATGGTTA+TGG - chr1_3:34678673-34678692 Msa0115730:CDS 40.0%
GGTTATGGTTCATTTCAGCT+CGG - chr1_3:34678688-34678707 Msa0115730:CDS 40.0%
! CGCATGCTTTGTTACATGAA+AGG - chr1_3:34678771-34678790 Msa0115730:five_prime_UTR 40.0%
ATGGTAGAAAGCAAGATGGA+AGG - chr1_3:34678805-34678824 Msa0115730:five_prime_UTR 40.0%
ACTAGCAGTAGAAGTTGAAG+AGG + chr1_3:34677406-34677425 None:intergenic 40.0%
AACTAGCTCATGAAGAAGTG+AGG + chr1_3:34677477-34677496 None:intergenic 40.0%
CTTCTTCATGAGCTAGTTGT+TGG - chr1_3:34677479-34677498 Msa0115730:three_prime_UTR 40.0%
AAGGTTACTCACCATGTTCT+TGG + chr1_3:34677750-34677769 None:intergenic 40.0%
!! GAGTGGAGAAAGTGTTTATG+CGG - chr1_3:34677994-34678013 Msa0115730:intron 40.0%
TGTTATCTATCCACCACATC+TGG - chr1_3:34678062-34678081 Msa0115730:CDS 40.0%
ATCAGAAAGGTCAAATGCGT+AGG - chr1_3:34678159-34678178 Msa0115730:CDS 40.0%
! ATCTGGGACTTACCTTATAG+CGG + chr1_3:34678187-34678206 None:intergenic 40.0%
! CAGTGGACAACTTTGATTTC+TGG - chr1_3:34678380-34678399 Msa0115730:intron 40.0%
TAATTCGGAGCACATGGTTA+TGG - chr1_3:34678673-34678692 Msa0115730:CDS 40.0%
GGTTATGGTTCATTTCAGCT+CGG - chr1_3:34678688-34678707 Msa0115730:CDS 40.0%
! CGCATGCTTTGTTACATGAA+AGG - chr1_3:34678771-34678790 Msa0115730:five_prime_UTR 40.0%
ATGGTAGAAAGCAAGATGGA+AGG - chr1_3:34678805-34678824 Msa0115730:five_prime_UTR 40.0%
! GGAATGTTCTGAGCTACAAG+AGG + chr1_3:34677385-34677404 None:intergenic 45.0%
! CGAACAAGAAAAGGCTTGCT+AGG - chr1_3:34677698-34677717 Msa0115730:three_prime_UTR 45.0%
!! CAAGAAAAGGCTTGCTAGGA+AGG - chr1_3:34677702-34677721 Msa0115730:three_prime_UTR 45.0%
AGGGGCTCAAATTCTTCAAG+TGG - chr1_3:34677968-34677987 Msa0115730:intron 45.0%
GGGGCTCAAATTCTTCAAGT+GGG - chr1_3:34677969-34677988 Msa0115730:intron 45.0%
GCTCAAATTCTTCAAGTGGG+TGG - chr1_3:34677972-34677991 Msa0115730:intron 45.0%
AATTCTTCAAGTGGGTGGAG+TGG - chr1_3:34677977-34677996 Msa0115730:intron 45.0%
!!! GCGGTATTTTAGTGTGAGTG+AGG - chr1_3:34678013-34678032 Msa0115730:intron 45.0%
AATGCGTAGGATCCGCTATA+AGG - chr1_3:34678172-34678191 Msa0115730:CDS 45.0%
! GGGCACTGTCTTTGATAAGA+AGG - chr1_3:34678598-34678617 Msa0115730:intron 45.0%
! GGAATGTTCTGAGCTACAAG+AGG + chr1_3:34677385-34677404 None:intergenic 45.0%
! CGAACAAGAAAAGGCTTGCT+AGG - chr1_3:34677698-34677717 Msa0115730:three_prime_UTR 45.0%
!! CAAGAAAAGGCTTGCTAGGA+AGG - chr1_3:34677702-34677721 Msa0115730:three_prime_UTR 45.0%
AGGGGCTCAAATTCTTCAAG+TGG - chr1_3:34677968-34677987 Msa0115730:intron 45.0%
GGGGCTCAAATTCTTCAAGT+GGG - chr1_3:34677969-34677988 Msa0115730:intron 45.0%
GCTCAAATTCTTCAAGTGGG+TGG - chr1_3:34677972-34677991 Msa0115730:intron 45.0%
AATTCTTCAAGTGGGTGGAG+TGG - chr1_3:34677977-34677996 Msa0115730:intron 45.0%
!!! GCGGTATTTTAGTGTGAGTG+AGG - chr1_3:34678013-34678032 Msa0115730:intron 45.0%
AATGCGTAGGATCCGCTATA+AGG - chr1_3:34678172-34678191 Msa0115730:CDS 45.0%
! GGGCACTGTCTTTGATAAGA+AGG - chr1_3:34678598-34678617 Msa0115730:intron 45.0%
!! ATTTAAAAATTATGTATAAT+AGG + chr1_3:34677894-34677913 None:intergenic 5.0%
!! ATTTAAAAATTATGTATAAT+AGG + chr1_3:34677894-34677913 None:intergenic 5.0%
TGAAGAGGAGACCAGCTAGA+GGG + chr1_3:34678088-34678107 None:intergenic 50.0%
ATGAAGAGGAGACCAGCTAG+AGG + chr1_3:34678089-34678108 None:intergenic 50.0%
CCTTCTCGTTCGAGATGAAG+AGG + chr1_3:34678103-34678122 None:intergenic 50.0%
CCTCTTCATCTCGAACGAGA+AGG - chr1_3:34678100-34678119 Msa0115730:CDS 50.0%
GAATGAAGCTTCACGTTGCG+AGG - chr1_3:34678519-34678538 Msa0115730:CDS 50.0%
TGAAGAGGAGACCAGCTAGA+GGG + chr1_3:34678088-34678107 None:intergenic 50.0%
ATGAAGAGGAGACCAGCTAG+AGG + chr1_3:34678089-34678108 None:intergenic 50.0%
CCTTCTCGTTCGAGATGAAG+AGG + chr1_3:34678103-34678122 None:intergenic 50.0%
CCTCTTCATCTCGAACGAGA+AGG - chr1_3:34678100-34678119 Msa0115730:CDS 50.0%
GAATGAAGCTTCACGTTGCG+AGG - chr1_3:34678519-34678538 Msa0115730:CDS 50.0%
AGCTAGAGGGCCAGATGTGG+TGG + chr1_3:34678075-34678094 None:intergenic 60.0%
ACCACATCTGGCCCTCTAGC+TGG - chr1_3:34678074-34678093 Msa0115730:CDS 60.0%
ACCAGCTAGAGGGCCAGATG+TGG + chr1_3:34678078-34678097 None:intergenic 60.0%
AGCTAGAGGGCCAGATGTGG+TGG + chr1_3:34678075-34678094 None:intergenic 60.0%
ACCACATCTGGCCCTCTAGC+TGG - chr1_3:34678074-34678093 Msa0115730:CDS 60.0%
ACCAGCTAGAGGGCCAGATG+TGG + chr1_3:34678078-34678097 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 34677382 34678853 34677382 ID=Msa0115730;Name=Msa0115730
chr1_3 mRNA 34677382 34678853 34677382 ID=Msa0115730-mRNA-1;Parent=Msa0115730;Name=Msa0115730-mRNA-1;_AED=0.06;_eAED=0.06;_QI=85|1|0.75|1|1|1|4|385|218
chr1_3 exon 34677382 34677940 34677382 ID=Msa0115730-mRNA-1:exon:10947;Parent=Msa0115730-mRNA-1
chr1_3 exon 34678042 34678316 34678042 ID=Msa0115730-mRNA-1:exon:10946;Parent=Msa0115730-mRNA-1
chr1_3 exon 34678478 34678549 34678478 ID=Msa0115730-mRNA-1:exon:10945;Parent=Msa0115730-mRNA-1
chr1_3 exon 34678633 34678853 34678633 ID=Msa0115730-mRNA-1:exon:10944;Parent=Msa0115730-mRNA-1
chr1_3 five_prime_UTR 34678769 34678853 34678769 ID=Msa0115730-mRNA-1:five_prime_utr;Parent=Msa0115730-mRNA-1
chr1_3 CDS 34678633 34678768 34678633 ID=Msa0115730-mRNA-1:cds;Parent=Msa0115730-mRNA-1
chr1_3 CDS 34678478 34678549 34678478 ID=Msa0115730-mRNA-1:cds;Parent=Msa0115730-mRNA-1
chr1_3 CDS 34678042 34678316 34678042 ID=Msa0115730-mRNA-1:cds;Parent=Msa0115730-mRNA-1
chr1_3 CDS 34677767 34677940 34677767 ID=Msa0115730-mRNA-1:cds;Parent=Msa0115730-mRNA-1
chr1_3 three_prime_UTR 34677382 34677766 34677382 ID=Msa0115730-mRNA-1:three_prime_utr;Parent=Msa0115730-mRNA-1
Gene Sequence

>Msa0115730

ATGCAGACCTCACTTCTTCATGAGCTAGTTGTTGGAACTCCAATCATATACCATCAGTTTCAAAATTCGGTTAACAAATACTTACTTGATTCTAACTCTCATCAATGTCAATATCCATCCAAACATCAAAGCAAAAGTTTAACAAATTCGAACAAGAAAAGGCTTGCTAGGAAGGCTGATAGTCTCTCACAAAAAGTCCAAGAACATGTAAGACTGAGAGCAAACATTTCTGAAACAATAAAGAGAAAATTGAGCTTAGGGGCTCAAATTCTTCAAGTGGGTGGAGTGGAGAAAGTGTTTATGCGGTATTTTAGTGTGAGTGAGGACGAGAATCTGTTGAAAATTTCACAGTGTTATCTATCCACCACATCTGGCCCTCTAGCTGGTCTCCTCTTCATCTCGAACGAGAAGGTTGCCTTTTGCAGTGACAGATCAATAAAAGTCTTTAATCAGAAAGGTCAAATGCGTAGGATCCGCTATAAGGTTGCCATTCCACTAAAGAATATCAAGTGTGTGAATGAAAGTCAAAATGTTGCGAAGCCAACGCAGAAGTACATAAATATAGTTACAGTGGACAACTTTGATTTCTGGCTTATGGGTGTCTTCAAATATCATAAAACTTTCAAATATCTTGAGCAGGCAATTTCTCAAGCTTAG
Protein sequence

>Msa0115730

MQTSLLHELVVGTPIIYHQFQNSVNKYLLDSNSHQCQYPSKHQSKSLTNSNKKRLARKADSLSQKVQEHVRLRANISETIKRKLSLGAQILQVGGVEKVFMRYFSVSEDENLLKISQCYLSTTSGPLAGLLFISNEKVAFCSDRSIKVFNQKGQMRRIRYKVAIPLKNIKCVNESQNVAKPTQKYINIVTVDNFDFWLMGVFKYHKTFKYLEQAISQA*