AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1150960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1150960 MtrunA17_Chr7g0240771 86.207 406 33 9 1 399 1 390 0.0 635
Msa1150960 MtrunA17_Chr4g0008811 29.781 366 171 16 51 399 9 305 5.96e-22 95.1
Msa1150960 MtrunA17_Chr5g0436151 30.670 388 192 19 49 399 15 362 2.81e-16 79.7
Msa1150960 MtrunA17_Chr2g0328681 38.583 127 54 5 278 399 214 321 2.01e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1150960 AT3G54000.1 30.000 410 207 20 5 399 8 352 5.81e-20 90.9
Msa1150960 AT3G54000.3 31.672 341 173 17 5 330 8 303 2.08e-19 88.6
Msa1150960 AT3G54000.2 28.788 330 178 15 5 322 8 292 9.71e-13 68.9

Find 110 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATAGAGGAAAAGAAAA+AGG 0.220430 7_4:-34821027 None:intergenic
CTATATAGTCTTCAAGCTTT+AGG 0.234041 7_4:-34822713 None:intergenic
CTCTCCCATGTTTCGCATTT+CGG 0.242451 7_4:+34820979 Msa1150960:five_prime_UTR
CAATCAACCCTATGTGCTTT+TGG 0.244653 7_4:+34821578 Msa1150960:CDS
GAGAAAATGTGAAGGGGTTT+TGG 0.250864 7_4:-34821066 None:intergenic
ACTGATATCACTGCAATTTC+AGG 0.266164 7_4:-34823353 None:intergenic
GTCTTCAAGCTTTAGGTTAA+GGG 0.268229 7_4:-34822706 None:intergenic
TGATGAACCTTTGCGGCATT+TGG 0.275294 7_4:-34821609 None:intergenic
CTGAAAGGCTGAACACAATA+AGG 0.279562 7_4:+34821966 Msa1150960:CDS
TCCACCTCTAATGTTGAAAA+TGG 0.300073 7_4:+34822774 Msa1150960:CDS
CTGTAATACCACTGCAAATC+TGG 0.300724 7_4:-34823544 None:intergenic
GCGGGTATGCAGAAGCAAAA+TGG 0.310089 7_4:+34821989 Msa1150960:CDS
AGTCTTCAAGCTTTAGGTTA+AGG 0.313871 7_4:-34822707 None:intergenic
TGCTGCTGGCTGAGGCCTGC+TGG 0.319876 7_4:-34823030 None:intergenic
TAATAATAATAGAAGATAAA+TGG 0.327212 7_4:+34823255 Msa1150960:three_prime_UTR
AACAACAACGTTGCTGGTTT+AGG 0.336273 7_4:+34822073 Msa1150960:CDS
GCATTCTCACAAACACCATT+AGG 0.337817 7_4:-34821644 None:intergenic
ATTTGGATGACGGTGAGTTC+TGG 0.349881 7_4:+34821155 Msa1150960:CDS
GGAAATTGAAGTGAAAGAAA+AGG 0.359094 7_4:-34821091 None:intergenic
ATTTCATTGCTCACTGCTGC+TGG 0.359817 7_4:-34823044 None:intergenic
AGGGTTGTGGAGTTTGAATT+TGG 0.367866 7_4:-34822141 None:intergenic
CGGGTATGCAGAAGCAAAAT+GGG 0.371790 7_4:+34821990 Msa1150960:CDS
TTGCTCACTGCTGCTGGCTG+AGG 0.373008 7_4:-34823038 None:intergenic
GTTATGATTCTCAGAGGCTT+TGG 0.379710 7_4:-34821425 None:intergenic
TTGTTCATCATCGTCACTTT+CGG 0.392470 7_4:-34821350 None:intergenic
CTGTTGCACCTCCAAGGCAT+AGG 0.394744 7_4:+34822964 Msa1150960:CDS
GTTGAATTTCTTGATTCAAC+AGG 0.398365 7_4:-34822232 None:intergenic
GTTTCGCATTTCGGTAATCA+TGG 0.406161 7_4:+34820988 Msa1150960:five_prime_UTR
TCAAGCCAAGGTAGCCCTAA+TGG 0.413695 7_4:+34821629 Msa1150960:CDS
AGCGAAAGCGAAGAAGGTTT+CGG 0.413750 7_4:-34821232 None:intergenic
GAAATTGAAGTGAAAGAAAA+GGG 0.418507 7_4:-34821090 None:intergenic
AATAATAATAAATTGGTTTG+TGG 0.433439 7_4:+34823489 Msa1150960:three_prime_UTR
AATGGTTATGGTAGCAGTAC+TGG 0.435118 7_4:+34822187 Msa1150960:CDS
GCAGCAGCAACAATACCAGC+AGG 0.436574 7_4:+34823015 Msa1150960:CDS
AGGGGATTTGATGTAACAAA+AGG 0.449526 7_4:+34823171 Msa1150960:three_prime_UTR
CATTCTCACAAACACCATTA+GGG 0.451029 7_4:-34821643 None:intergenic
GGGGAAGAAGAATTTCTTTG+AGG 0.452557 7_4:-34821746 None:intergenic
AAATAATGTTAGGAGCCTCT+TGG 0.453899 7_4:+34823301 Msa1150960:three_prime_UTR
AAAGCTTGAAGACTATATAG+TGG 0.470534 7_4:+34822716 Msa1150960:CDS
CTTGGCTTGATGAACCTTTG+CGG 0.475577 7_4:-34821616 None:intergenic
ACATTATTACCCCTATGCCT+TGG 0.482077 7_4:-34822975 None:intergenic
TTGAAAAGCGAAAGCGAAGA+AGG 0.482963 7_4:-34821238 None:intergenic
GGAGGAAGAAATGTTGTTGA+TGG 0.488016 7_4:+34822040 Msa1150960:CDS
TTATTACCCCTATGCCTTGG+AGG 0.489609 7_4:-34822972 None:intergenic
TTATTCATAGTAAGTAACTA+AGG 0.495204 7_4:-34823593 None:intergenic
TGTTGCACCTCCAAGGCATA+GGG 0.498023 7_4:+34822965 Msa1150960:CDS
TGTTGAGCATGTTGTAGTGT+TGG 0.499882 7_4:-34822115 None:intergenic
GTCATTTCCAAAAGCACATA+GGG 0.503258 7_4:-34821585 None:intergenic
ATGATGAAAATGATATGAAA+TGG 0.513671 7_4:+34823100 Msa1150960:three_prime_UTR
AAACACCATTAGGGCTACCT+TGG 0.514969 7_4:-34821634 None:intergenic
AAATGGTCCTTTGATTTGCT+TGG 0.515224 7_4:+34823117 Msa1150960:three_prime_UTR
TTTCAGCAAAGACAAACAAA+TGG 0.515645 7_4:+34822019 Msa1150960:CDS
TTTCGCATTTCGGTAATCAT+GGG 0.519762 7_4:+34820989 Msa1150960:five_prime_UTR
TAGGTTTGTCATCATCTGCA+TGG 0.523719 7_4:+34822092 Msa1150960:CDS
GATTACCGAAATGCGAAACA+TGG 0.528517 7_4:-34820984 None:intergenic
TCAGAGCAAGGGAATCCATA+AGG 0.528814 7_4:-34821286 None:intergenic
ACAGCTCTCATGTAGGGTTG+TGG 0.529286 7_4:-34822154 None:intergenic
GTCATCCAAATTCTCAGCCA+TGG 0.531206 7_4:-34821143 None:intergenic
TGAAAGGCTGAACACAATAA+GGG 0.531358 7_4:+34821967 Msa1150960:CDS
AGTAAGTAACTAAGGTGGAC+AGG 0.531804 7_4:-34823585 None:intergenic
GAATCATAACAACAATAACT+TGG 0.540471 7_4:+34821438 Msa1150960:CDS
AAAGGGAGAGAAAATGTGAA+GGG 0.545860 7_4:-34821073 None:intergenic
TGCATTTGTTCCAGCTAGAG+TGG 0.546208 7_4:+34822677 Msa1150960:CDS
AAGCTTGAAGACTATATAGT+GGG 0.550779 7_4:+34822717 Msa1150960:CDS
AGTCATTTCCAAAAGCACAT+AGG 0.552956 7_4:-34821586 None:intergenic
GGACAGGTTGGCATCAAAGA+AGG 0.554332 7_4:-34823569 None:intergenic
TGTGTCGTCACCGTCGTCGT+CGG 0.554477 7_4:-34821197 None:intergenic
AGGAAAACAGCTCTCATGTA+GGG 0.560500 7_4:-34822160 None:intergenic
GGCTTTGACCAGATTTGCAG+TGG 0.562137 7_4:+34823536 Msa1150960:three_prime_UTR
CAGAGGCTTTGGAATCAACA+TGG 0.562511 7_4:-34821414 None:intergenic
TAGCTGCTGTTGCACCTCCA+AGG 0.563262 7_4:+34822958 Msa1150960:CDS
AAGGAAAACAGCTCTCATGT+AGG 0.567572 7_4:-34822161 None:intergenic
TTTCTTGATTCAACAGGACG+AGG 0.572592 7_4:-34822226 None:intergenic
AAATCAAGCTTCCACAGGAG+TGG 0.577332 7_4:+34823063 Msa1150960:CDS
AGTAACTAAGGTGGACAGGT+TGG 0.585188 7_4:-34823581 None:intergenic
CAATGAAATCAAGCTTCCAC+AGG 0.589243 7_4:+34823058 Msa1150960:CDS
AATGCTACCAAGCAAATCAA+AGG 0.589835 7_4:-34823124 None:intergenic
TGATAATGAGAAAGTGATTG+TGG 0.597158 7_4:-34821818 None:intergenic
AAAAGGGAGAGAAAATGTGA+AGG 0.597677 7_4:-34821074 None:intergenic
TATGGTAGCAGTACTGGCAC+TGG 0.599660 7_4:+34822193 Msa1150960:CDS
AATGACTCCAAATGCCGCAA+AGG 0.600691 7_4:+34821602 Msa1150960:CDS
CATCAGTACGTCCACTCCTG+TGG 0.602987 7_4:-34823074 None:intergenic
CGCAAAGGTTCATCAAGCCA+AGG 0.603040 7_4:+34821617 Msa1150960:CDS
GATGCAAGTGTTGTTGCGGC+TGG 0.606152 7_4:-34822743 None:intergenic
AGGCTGAACACAATAAGGGC+GGG 0.606490 7_4:+34821971 Msa1150960:CDS
AAGGGAGAGAAAATGTGAAG+GGG 0.606991 7_4:-34821072 None:intergenic
CAGCAAAGACAAACAAATGG+AGG 0.610080 7_4:+34822022 Msa1150960:CDS
GGTCATTAGAAAGTCTCTCA+AGG 0.612790 7_4:+34823399 Msa1150960:three_prime_UTR
ATTACCGAAATGCGAAACAT+GGG 0.619209 7_4:-34820983 None:intergenic
TTACTATGAATAATAATACA+AGG 0.623391 7_4:+34823603 Msa1150960:three_prime_UTR
ATTGTTGTTATGATTCTCAG+AGG 0.625609 7_4:-34821431 None:intergenic
GGTGAACTGAGATCAGAGCA+AGG 0.626730 7_4:-34821298 None:intergenic
TTTGGAATCAACATGGAGAG+AGG 0.627829 7_4:-34821407 None:intergenic
GGCAACAACAAATTGCTGAA+AGG 0.630304 7_4:+34821951 Msa1150960:CDS
GAAGCAGCAAGAGAATCGAT+TGG 0.636063 7_4:-34821319 None:intergenic
TTTGGAAGGTAAAGTAACAG+GGG 0.638650 7_4:-34821765 None:intergenic
GGGTATGCAGAAGCAAAATG+GGG 0.639834 7_4:+34821991 Msa1150960:CDS
CAGTTTCTCTCCGACGACGA+CGG 0.644936 7_4:+34821187 Msa1150960:CDS
GTGAACTGAGATCAGAGCAA+GGG 0.649275 7_4:-34821297 None:intergenic
AAACGATGCAAGTGTTGTTG+CGG 0.650274 7_4:-34822747 None:intergenic
TGCTACCATAACCATTACCA+AGG 0.652239 7_4:-34822180 None:intergenic
TTCATAGTAAGTAACTAAGG+TGG 0.653746 7_4:-34823590 None:intergenic
AAAGCACATAGGGTTGATTG+TGG 0.656361 7_4:-34821575 None:intergenic
ATGGCTGAGAATTTGGATGA+CGG 0.669713 7_4:+34821145 Msa1150960:CDS
AAGGCTGAACACAATAAGGG+CGG 0.671077 7_4:+34821970 Msa1150960:CDS
GGTATGCAGAAGCAAAATGG+GGG 0.674755 7_4:+34821992 Msa1150960:CDS
GTTGCACCTCCAAGGCATAG+GGG 0.686212 7_4:+34822966 Msa1150960:CDS
AGCTTGAAGACTATATAGTG+GGG 0.692039 7_4:+34822718 Msa1150960:CDS
TCGTCGGAGAGAAACTGCGG+CGG 0.707718 7_4:-34821181 None:intergenic
TCGTCGTCGGAGAGAAACTG+CGG 0.728597 7_4:-34821184 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATACAGTTTATAA+GGG - chr7_4:34822868-34822887 None:intergenic 10.0%
!! ATAAATAAATACAGTTTATA+AGG - chr7_4:34822869-34822888 None:intergenic 10.0%
!! ATAAACTGTATTTATTTATT+TGG + chr7_4:34822870-34822889 Msa1150960:intron 10.0%
!! TAAACTGTATTTATTTATTT+GGG + chr7_4:34822871-34822890 Msa1150960:intron 10.0%
!! TAATAATAATAGAAGATAAA+TGG + chr7_4:34823255-34823274 Msa1150960:three_prime_UTR 10.0%
!!! TTTTTTTTGTTTTTTCCTTT+TGG + chr7_4:34821480-34821499 Msa1150960:intron 15.0%
!!! TTTTTTTGTTTTTTCCTTTT+GGG + chr7_4:34821481-34821500 Msa1150960:intron 15.0%
!!! AAATGTCGTATTTTTAATAA+TGG + chr7_4:34821875-34821894 Msa1150960:intron 15.0%
!!! AATGTCGTATTTTTAATAAT+GGG + chr7_4:34821876-34821895 Msa1150960:intron 15.0%
!! AAATTATTAAATATCACAAC+CGG - chr7_4:34822549-34822568 None:intergenic 15.0%
!! AAACTGTATTTATTTATTTG+GGG + chr7_4:34822872-34822891 Msa1150960:intron 15.0%
!!! TTTTGTTTATCTTTTTTGTT+TGG + chr7_4:34823451-34823470 Msa1150960:three_prime_UTR 15.0%
!!! TTTTTTGTTTGGTTTTATTA+GGG + chr7_4:34823462-34823481 Msa1150960:three_prime_UTR 15.0%
!! GGGTAATAATAATAATAAAT+TGG + chr7_4:34823482-34823501 Msa1150960:three_prime_UTR 15.0%
!!! AATAATAATAAATTGGTTTG+TGG + chr7_4:34823489-34823508 Msa1150960:three_prime_UTR 15.0%
!! TTAATAGAGGAAAAGAAAAA+GGG - chr7_4:34821029-34821048 None:intergenic 20.0%
!! TTTAATAGAGGAAAAGAAAA+AGG - chr7_4:34821030-34821049 None:intergenic 20.0%
!!! TTTTTTGTTTTTTCCTTTTG+GGG + chr7_4:34821482-34821501 Msa1150960:intron 20.0%
!! AAAAATAATAAAACCCCAAA+AGG - chr7_4:34821498-34821517 None:intergenic 20.0%
!! AAAATTAACATCATCATCAT+GGG - chr7_4:34822283-34822302 None:intergenic 20.0%
!!! AATGTTTTAATGCATTTAAG+AGG + chr7_4:34822355-34822374 Msa1150960:intron 20.0%
!!! ATGTTTTAATGCATTTAAGA+GGG + chr7_4:34822356-34822375 Msa1150960:intron 20.0%
!!! TTTAAAATGTTACAACACAA+TGG + chr7_4:34822405-34822424 Msa1150960:intron 20.0%
!!! TTTTAATTGTTTTGAATGTG+TGG + chr7_4:34822522-34822541 Msa1150960:intron 20.0%
!!! AACTTTACCAATTTTTTTTG+TGG + chr7_4:34822629-34822648 Msa1150960:intron 20.0%
!! ACAAAAACCACAAAAAAAAT+TGG - chr7_4:34822639-34822658 None:intergenic 20.0%
!! AACTGTATTTATTTATTTGG+GGG + chr7_4:34822873-34822892 Msa1150960:intron 20.0%
!! ATGATGAAAATGATATGAAA+TGG + chr7_4:34823100-34823119 Msa1150960:three_prime_UTR 20.0%
!!! AGTTTTATAGAAGAAACAAT+AGG + chr7_4:34823199-34823218 Msa1150960:three_prime_UTR 20.0%
!!! CTTTTTTGTTTGGTTTTATT+AGG + chr7_4:34823461-34823480 Msa1150960:three_prime_UTR 20.0%
!! AGACAATGAAAAAATTAAGA+AGG + chr7_4:34823513-34823532 Msa1150960:three_prime_UTR 20.0%
!! GACAATGAAAAAATTAAGAA+GGG + chr7_4:34823514-34823533 Msa1150960:three_prime_UTR 20.0%
!! ACAATGAAAAAATTAAGAAG+GGG + chr7_4:34823515-34823534 Msa1150960:three_prime_UTR 20.0%
!! TTATTCATAGTAAGTAACTA+AGG - chr7_4:34823596-34823615 None:intergenic 20.0%
!!! GTTTGTTAGGATTTTAATAG+AGG - chr7_4:34821042-34821061 None:intergenic 25.0%
! GAAATTGAAGTGAAAGAAAA+GGG - chr7_4:34821093-34821112 None:intergenic 25.0%
! GAATCATAACAACAATAACT+TGG + chr7_4:34821438-34821457 Msa1150960:CDS 25.0%
! AGAAATTCAACAAAAAAACC+AGG + chr7_4:34822244-34822263 Msa1150960:CDS 25.0%
! CAAAATTAACATCATCATCA+TGG - chr7_4:34822284-34822303 None:intergenic 25.0%
! CTAAATCATAAACGAAAAAC+GGG - chr7_4:34822472-34822491 None:intergenic 25.0%
! CCTAAATCATAAACGAAAAA+CGG - chr7_4:34822473-34822492 None:intergenic 25.0%
! ACTGTATTTATTTATTTGGG+GGG + chr7_4:34822874-34822893 Msa1150960:intron 25.0%
!!! AAGAAACAATAGGTCTTTTT+TGG + chr7_4:34823209-34823228 Msa1150960:three_prime_UTR 25.0%
! AAGAGGCTAAAAATAATGTT+AGG + chr7_4:34823291-34823310 Msa1150960:three_prime_UTR 25.0%
!! ACTTTTTGTTCTTTTTGATG+AGG + chr7_4:34823377-34823396 Msa1150960:three_prime_UTR 25.0%
!! CTTTTTGTTCTTTTTGATGA+GGG + chr7_4:34823378-34823397 Msa1150960:three_prime_UTR 25.0%
GAAAAGAAAAAGGGATTATG+TGG - chr7_4:34821020-34821039 None:intergenic 30.0%
GGAAATTGAAGTGAAAGAAA+AGG - chr7_4:34821094-34821113 None:intergenic 30.0%
AACAGAGAGAAAAAAAACAG+AGG - chr7_4:34821115-34821134 None:intergenic 30.0%
! ATTGTTGTTATGATTCTCAG+AGG - chr7_4:34821434-34821453 None:intergenic 30.0%
!!! GTTTTTATTGTCGTTTTGCA+GGG + chr7_4:34821545-34821564 Msa1150960:intron 30.0%
!! TTTTGGAAGGTAAAGTAACA+GGG - chr7_4:34821769-34821788 None:intergenic 30.0%
!! TTTTTGGAAGGTAAAGTAAC+AGG - chr7_4:34821770-34821789 None:intergenic 30.0%
! TGATAATGAGAAAGTGATTG+TGG - chr7_4:34821821-34821840 None:intergenic 30.0%
TTTCAGCAAAGACAAACAAA+TGG + chr7_4:34822019-34822038 Msa1150960:CDS 30.0%
GTTGAATTTCTTGATTCAAC+AGG - chr7_4:34822235-34822254 None:intergenic 30.0%
!!! TGTACGTTTTTTTGCGTTTT+TGG + chr7_4:34822328-34822347 Msa1150960:intron 30.0%
! TTTTAATGCATTTAAGAGGG+TGG + chr7_4:34822359-34822378 Msa1150960:intron 30.0%
!! CCGTTTTTCGTTTATGATTT+AGG + chr7_4:34822470-34822489 Msa1150960:intron 30.0%
! TTAATGTACGTTTTTGCTAC+TGG + chr7_4:34822499-34822518 Msa1150960:intron 30.0%
CTATATAGTCTTCAAGCTTT+AGG - chr7_4:34822716-34822735 None:intergenic 30.0%
AAAGCTTGAAGACTATATAG+TGG + chr7_4:34822716-34822735 Msa1150960:CDS 30.0%
AAGCTTGAAGACTATATAGT+GGG + chr7_4:34822717-34822736 Msa1150960:CDS 30.0%
! AGTACCATTTTCAACATTAG+AGG - chr7_4:34822781-34822800 None:intergenic 30.0%
GGGGGTTTATTTAATCAAAA+GGG + chr7_4:34822892-34822911 Msa1150960:intron 30.0%
GGGGTTTATTTAATCAAAAG+GGG + chr7_4:34822893-34822912 Msa1150960:intron 30.0%
GGGTTTATTTAATCAAAAGG+GGG + chr7_4:34822894-34822913 Msa1150960:intron 30.0%
GGTTTATTTAATCAAAAGGG+GGG + chr7_4:34822895-34822914 Msa1150960:intron 30.0%
!! ATTTGCTTGGTAGCATTTTA+AGG + chr7_4:34823130-34823149 Msa1150960:three_prime_UTR 30.0%
!! TTTGCTTGGTAGCATTTTAA+GGG + chr7_4:34823131-34823150 Msa1150960:three_prime_UTR 30.0%
!!! TCTTTTTTGGATGAAGATGT+AGG + chr7_4:34823222-34823241 Msa1150960:three_prime_UTR 30.0%
!!! ATGGTTTTTTGTTTAGCAAG+AGG + chr7_4:34823274-34823293 Msa1150960:three_prime_UTR 30.0%
! TCACTTCTTTTTAATCCAAG+AGG - chr7_4:34823319-34823338 None:intergenic 30.0%
TTCATAGTAAGTAACTAAGG+TGG - chr7_4:34823593-34823612 None:intergenic 30.0%
ATTACCGAAATGCGAAACAT+GGG - chr7_4:34820986-34821005 None:intergenic 35.0%
TTTCGCATTTCGGTAATCAT+GGG + chr7_4:34820989-34821008 Msa1150960:five_prime_UTR 35.0%
AGAAAAAGGGATTATGTGGA+GGG - chr7_4:34821016-34821035 None:intergenic 35.0%
AAGAAAAAGGGATTATGTGG+AGG - chr7_4:34821017-34821036 None:intergenic 35.0%
AAAGGGAGAGAAAATGTGAA+GGG - chr7_4:34821076-34821095 None:intergenic 35.0%
AAAAGGGAGAGAAAATGTGA+AGG - chr7_4:34821077-34821096 None:intergenic 35.0%
! CTCTCTGTTTTCATTTTCCA+TGG + chr7_4:34821126-34821145 Msa1150960:exon 35.0%
! ATTTTCCATGGCTGAGAATT+TGG + chr7_4:34821138-34821157 Msa1150960:exon 35.0%
AATCCATAAGGAAAAAGCAG+AGG - chr7_4:34821277-34821296 None:intergenic 35.0%
! TTGTTCATCATCGTCACTTT+CGG - chr7_4:34821353-34821372 None:intergenic 35.0%
!!! CGTTTTTATTGTCGTTTTGC+AGG + chr7_4:34821544-34821563 Msa1150960:intron 35.0%
! GTCATTTCCAAAAGCACATA+GGG - chr7_4:34821588-34821607 None:intergenic 35.0%
! AGTCATTTCCAAAAGCACAT+AGG - chr7_4:34821589-34821608 None:intergenic 35.0%
CATTCTCACAAACACCATTA+GGG - chr7_4:34821646-34821665 None:intergenic 35.0%
TTTGGAAGGTAAAGTAACAG+GGG - chr7_4:34821768-34821787 None:intergenic 35.0%
CAAAAAAGCTCAAATCAGCA+GGG - chr7_4:34821796-34821815 None:intergenic 35.0%
!!! GTTGATGATTTTAGTTGCAG+AGG + chr7_4:34821924-34821943 Msa1150960:intron 35.0%
!!! GATTTTAGTTGCAGAGGATT+AGG + chr7_4:34821930-34821949 Msa1150960:intron 35.0%
TGAAAGGCTGAACACAATAA+GGG + chr7_4:34821967-34821986 Msa1150960:CDS 35.0%
!!! GTTTTCCTTGGTAATGGTTA+TGG + chr7_4:34822175-34822194 Msa1150960:CDS 35.0%
ATGGGAAAAAAGTAATCACC+TGG - chr7_4:34822265-34822284 None:intergenic 35.0%
!!! ATTGTTTTGAATGTGTGGAC+CGG + chr7_4:34822527-34822546 Msa1150960:intron 35.0%
!! GTCTTCAAGCTTTAGGTTAA+GGG - chr7_4:34822709-34822728 None:intergenic 35.0%
!! AGTCTTCAAGCTTTAGGTTA+AGG - chr7_4:34822710-34822729 None:intergenic 35.0%
AGCTTGAAGACTATATAGTG+GGG + chr7_4:34822718-34822737 Msa1150960:CDS 35.0%
TCCACCTCTAATGTTGAAAA+TGG + chr7_4:34822774-34822793 Msa1150960:CDS 35.0%
! ACCATTTTCAACATTAGAGG+TGG - chr7_4:34822778-34822797 None:intergenic 35.0%
GGGGGGTTTATTTAATCAAA+AGG + chr7_4:34822891-34822910 Msa1150960:intron 35.0%
!! AAATGGTCCTTTGATTTGCT+TGG + chr7_4:34823117-34823136 Msa1150960:three_prime_UTR 35.0%
AATGCTACCAAGCAAATCAA+AGG - chr7_4:34823127-34823146 None:intergenic 35.0%
!! TGGTAGCATTTTAAGGGATT+TGG + chr7_4:34823137-34823156 Msa1150960:three_prime_UTR 35.0%
!! GGTAGCATTTTAAGGGATTT+GGG + chr7_4:34823138-34823157 Msa1150960:three_prime_UTR 35.0%
!! GTAGCATTTTAAGGGATTTG+GGG + chr7_4:34823139-34823158 Msa1150960:three_prime_UTR 35.0%
!! TAGCATTTTAAGGGATTTGG+GGG + chr7_4:34823140-34823159 Msa1150960:three_prime_UTR 35.0%
AGGGGATTTGATGTAACAAA+AGG + chr7_4:34823171-34823190 Msa1150960:three_prime_UTR 35.0%
AAATAATGTTAGGAGCCTCT+TGG + chr7_4:34823301-34823320 Msa1150960:three_prime_UTR 35.0%
ACTGATATCACTGCAATTTC+AGG - chr7_4:34823356-34823375 None:intergenic 35.0%
CTCTCAAGGAAAGACAAAAA+TGG + chr7_4:34823413-34823432 Msa1150960:three_prime_UTR 35.0%
TCAAGGAAAGACAAAAATGG+TGG + chr7_4:34823416-34823435 Msa1150960:three_prime_UTR 35.0%
GATTACCGAAATGCGAAACA+TGG - chr7_4:34820987-34821006 None:intergenic 40.0%
GTTTCGCATTTCGGTAATCA+TGG + chr7_4:34820988-34821007 Msa1150960:five_prime_UTR 40.0%
!!! GGGTTTTGGTTAGGTTTGTT+AGG - chr7_4:34821055-34821074 None:intergenic 40.0%
!!! AATGTGAAGGGGTTTTGGTT+AGG - chr7_4:34821064-34821083 None:intergenic 40.0%
! GAGAAAATGTGAAGGGGTTT+TGG - chr7_4:34821069-34821088 None:intergenic 40.0%
AAGGGAGAGAAAATGTGAAG+GGG - chr7_4:34821075-34821094 None:intergenic 40.0%
ATGGCTGAGAATTTGGATGA+CGG + chr7_4:34821145-34821164 Msa1150960:CDS 40.0%
TTGAAAAGCGAAAGCGAAGA+AGG - chr7_4:34821241-34821260 None:intergenic 40.0%
!! TCGCTTTCGCTTTTCAACAA+TGG + chr7_4:34821244-34821263 Msa1150960:CDS 40.0%
!!! GATCCTCTGCTTTTTCCTTA+TGG + chr7_4:34821271-34821290 Msa1150960:CDS 40.0%
! TTTGGAATCAACATGGAGAG+AGG - chr7_4:34821410-34821429 None:intergenic 40.0%
GTTATGATTCTCAGAGGCTT+TGG - chr7_4:34821428-34821447 None:intergenic 40.0%
!!! TTTATTGTCGTTTTGCAGGG+AGG + chr7_4:34821548-34821567 Msa1150960:intron 40.0%
! AAAGCACATAGGGTTGATTG+TGG - chr7_4:34821578-34821597 None:intergenic 40.0%
! CAATCAACCCTATGTGCTTT+TGG + chr7_4:34821578-34821597 Msa1150960:CDS 40.0%
GCATTCTCACAAACACCATT+AGG - chr7_4:34821647-34821666 None:intergenic 40.0%
GGGGAAGAAGAATTTCTTTG+AGG - chr7_4:34821749-34821768 None:intergenic 40.0%
!! CAAATCAGCAGGGTTGTTTT+TGG - chr7_4:34821786-34821805 None:intergenic 40.0%
GCAAAAAAGCTCAAATCAGC+AGG - chr7_4:34821797-34821816 None:intergenic 40.0%
GGCAACAACAAATTGCTGAA+AGG + chr7_4:34821951-34821970 Msa1150960:CDS 40.0%
CTGAAAGGCTGAACACAATA+AGG + chr7_4:34821966-34821985 Msa1150960:CDS 40.0%
CAGCAAAGACAAACAAATGG+AGG + chr7_4:34822022-34822041 Msa1150960:CDS 40.0%
GGAGGAAGAAATGTTGTTGA+TGG + chr7_4:34822040-34822059 Msa1150960:CDS 40.0%
AACAACAACAACAACGTTGC+TGG + chr7_4:34822067-34822086 Msa1150960:CDS 40.0%
!! AACAACAACGTTGCTGGTTT+AGG + chr7_4:34822073-34822092 Msa1150960:CDS 40.0%
TAGGTTTGTCATCATCTGCA+TGG + chr7_4:34822092-34822111 Msa1150960:CDS 40.0%
TGTTGAGCATGTTGTAGTGT+TGG - chr7_4:34822118-34822137 None:intergenic 40.0%
AGGGTTGTGGAGTTTGAATT+TGG - chr7_4:34822144-34822163 None:intergenic 40.0%
AGGAAAACAGCTCTCATGTA+GGG - chr7_4:34822163-34822182 None:intergenic 40.0%
AAGGAAAACAGCTCTCATGT+AGG - chr7_4:34822164-34822183 None:intergenic 40.0%
!! TACATGAGAGCTGTTTTCCT+TGG + chr7_4:34822163-34822182 Msa1150960:CDS 40.0%
!! AGAGCTGTTTTCCTTGGTAA+TGG + chr7_4:34822169-34822188 Msa1150960:CDS 40.0%
TGCTACCATAACCATTACCA+AGG - chr7_4:34822183-34822202 None:intergenic 40.0%
AATGGTTATGGTAGCAGTAC+TGG + chr7_4:34822187-34822206 Msa1150960:CDS 40.0%
TTTCTTGATTCAACAGGACG+AGG - chr7_4:34822229-34822248 None:intergenic 40.0%
! AAACGATGCAAGTGTTGTTG+CGG - chr7_4:34822750-34822769 None:intergenic 40.0%
ACATTATTACCCCTATGCCT+TGG - chr7_4:34822978-34822997 None:intergenic 40.0%
CAATGAAATCAAGCTTCCAC+AGG + chr7_4:34823058-34823077 Msa1150960:CDS 40.0%
!!! GGATTTGGGGGAGTATTTTA+GGG + chr7_4:34823152-34823171 Msa1150960:three_prime_UTR 40.0%
!!! GATTTGGGGGAGTATTTTAG+GGG + chr7_4:34823153-34823172 Msa1150960:three_prime_UTR 40.0%
GGTCATTAGAAAGTCTCTCA+AGG + chr7_4:34823399-34823418 Msa1150960:three_prime_UTR 40.0%
AGGAAAGACAAAAATGGTGG+TGG + chr7_4:34823419-34823438 Msa1150960:three_prime_UTR 40.0%
!! AAGACAAAAATGGTGGTGGA+TGG + chr7_4:34823423-34823442 Msa1150960:three_prime_UTR 40.0%
!! AGACAAAAATGGTGGTGGAT+GGG + chr7_4:34823424-34823443 Msa1150960:three_prime_UTR 40.0%
CTGTAATACCACTGCAAATC+TGG - chr7_4:34823547-34823566 None:intergenic 40.0%
AGTAAGTAACTAAGGTGGAC+AGG - chr7_4:34823588-34823607 None:intergenic 40.0%
CTCTCCCATGTTTCGCATTT+CGG + chr7_4:34820979-34820998 Msa1150960:five_prime_UTR 45.0%
GTCATCCAAATTCTCAGCCA+TGG - chr7_4:34821146-34821165 None:intergenic 45.0%
! ATTTGGATGACGGTGAGTTC+TGG + chr7_4:34821155-34821174 Msa1150960:CDS 45.0%
!! AGAAGGTTTCGGTTTTGTGG+TGG - chr7_4:34821224-34821243 None:intergenic 45.0%
! CGAAGAAGGTTTCGGTTTTG+TGG - chr7_4:34821227-34821246 None:intergenic 45.0%
AGCGAAAGCGAAGAAGGTTT+CGG - chr7_4:34821235-34821254 None:intergenic 45.0%
TCAGAGCAAGGGAATCCATA+AGG - chr7_4:34821289-34821308 None:intergenic 45.0%
GTGAACTGAGATCAGAGCAA+GGG - chr7_4:34821300-34821319 None:intergenic 45.0%
GAAGCAGCAAGAGAATCGAT+TGG - chr7_4:34821322-34821341 None:intergenic 45.0%
!! CAGAGGCTTTGGAATCAACA+TGG - chr7_4:34821417-34821436 None:intergenic 45.0%
AATGACTCCAAATGCCGCAA+AGG + chr7_4:34821602-34821621 Msa1150960:CDS 45.0%
TGATGAACCTTTGCGGCATT+TGG - chr7_4:34821612-34821631 None:intergenic 45.0%
! CTTGGCTTGATGAACCTTTG+CGG - chr7_4:34821619-34821638 None:intergenic 45.0%
AAACACCATTAGGGCTACCT+TGG - chr7_4:34821637-34821656 None:intergenic 45.0%
!!! TCAGCAGGGTTGTTTTTGGA+AGG - chr7_4:34821782-34821801 None:intergenic 45.0%
AAGGCTGAACACAATAAGGG+CGG + chr7_4:34821970-34821989 Msa1150960:CDS 45.0%
CGGGTATGCAGAAGCAAAAT+GGG + chr7_4:34821990-34822009 Msa1150960:CDS 45.0%
GGGTATGCAGAAGCAAAATG+GGG + chr7_4:34821991-34822010 Msa1150960:CDS 45.0%
GGTATGCAGAAGCAAAATGG+GGG + chr7_4:34821992-34822011 Msa1150960:CDS 45.0%
TGCATTTGTTCCAGCTAGAG+TGG + chr7_4:34822677-34822696 Msa1150960:CDS 45.0%
TTATTACCCCTATGCCTTGG+AGG - chr7_4:34822975-34822994 None:intergenic 45.0%
ATTTCATTGCTCACTGCTGC+TGG - chr7_4:34823047-34823066 None:intergenic 45.0%
AAATCAAGCTTCCACAGGAG+TGG + chr7_4:34823063-34823082 Msa1150960:CDS 45.0%
!!! GGGATTTGGGGGAGTATTTT+AGG + chr7_4:34823151-34823170 Msa1150960:three_prime_UTR 45.0%
AGTAACTAAGGTGGACAGGT+TGG - chr7_4:34823584-34823603 None:intergenic 45.0%
!!! TTTATTATTTTTTTACTTTT+AGG + chr7_4:34821506-34821525 Msa1150960:intron 5.0%
!!! TTATTATTTTTTTACTTTTA+GGG + chr7_4:34821507-34821526 Msa1150960:intron 5.0%
GGTGAACTGAGATCAGAGCA+AGG - chr7_4:34821301-34821320 None:intergenic 50.0%
CGCAAAGGTTCATCAAGCCA+AGG + chr7_4:34821617-34821636 Msa1150960:CDS 50.0%
TCAAGCCAAGGTAGCCCTAA+TGG + chr7_4:34821629-34821648 Msa1150960:CDS 50.0%
AGGCTGAACACAATAAGGGC+GGG + chr7_4:34821971-34821990 Msa1150960:CDS 50.0%
GCGGGTATGCAGAAGCAAAA+TGG + chr7_4:34821989-34822008 Msa1150960:CDS 50.0%
ACAGCTCTCATGTAGGGTTG+TGG - chr7_4:34822157-34822176 None:intergenic 50.0%
!! TATGGTAGCAGTACTGGCAC+TGG + chr7_4:34822193-34822212 Msa1150960:CDS 50.0%
TGTTGCACCTCCAAGGCATA+GGG + chr7_4:34822965-34822984 Msa1150960:CDS 50.0%
! GGCTTTGACCAGATTTGCAG+TGG + chr7_4:34823536-34823555 Msa1150960:three_prime_UTR 50.0%
! GGACAGGTTGGCATCAAAGA+AGG - chr7_4:34823572-34823591 None:intergenic 50.0%
TCGTCGTCGGAGAGAAACTG+CGG - chr7_4:34821187-34821206 None:intergenic 55.0%
CAGTTTCTCTCCGACGACGA+CGG + chr7_4:34821187-34821206 Msa1150960:CDS 55.0%
! GATGCAAGTGTTGTTGCGGC+TGG - chr7_4:34822746-34822765 None:intergenic 55.0%
TAGCTGCTGTTGCACCTCCA+AGG + chr7_4:34822958-34822977 Msa1150960:CDS 55.0%
CTGTTGCACCTCCAAGGCAT+AGG + chr7_4:34822964-34822983 Msa1150960:CDS 55.0%
GTTGCACCTCCAAGGCATAG+GGG + chr7_4:34822966-34822985 Msa1150960:CDS 55.0%
GCAGCAGCAACAATACCAGC+AGG + chr7_4:34823015-34823034 Msa1150960:CDS 55.0%
CATCAGTACGTCCACTCCTG+TGG - chr7_4:34823077-34823096 None:intergenic 55.0%
TCGTCGGAGAGAAACTGCGG+CGG - chr7_4:34821184-34821203 None:intergenic 60.0%
TGTGTCGTCACCGTCGTCGT+CGG - chr7_4:34821200-34821219 None:intergenic 60.0%
! TTGCTCACTGCTGCTGGCTG+AGG - chr7_4:34823041-34823060 None:intergenic 60.0%
AGGGCATGCGCCACTCTAGC+TGG - chr7_4:34822690-34822709 None:intergenic 65.0%
! TGCTGCTGGCTGAGGCCTGC+TGG - chr7_4:34823033-34823052 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 34820956 34823624 34820956 ID=Msa1150960;Name=Msa1150960
chr7_4 mRNA 34820956 34823624 34820956 ID=Msa1150960-mRNA-1;Parent=Msa1150960;Name=Msa1150960-mRNA-1;_AED=0.13;_eAED=0.13;_QI=189|1|1|1|1|1|5|530|399
chr7_4 exon 34820956 34821459 34820956 ID=Msa1150960-mRNA-1:exon:8258;Parent=Msa1150960-mRNA-1
chr7_4 exon 34821566 34821856 34821566 ID=Msa1150960-mRNA-1:exon:8259;Parent=Msa1150960-mRNA-1
chr7_4 exon 34821938 34822265 34821938 ID=Msa1150960-mRNA-1:exon:8260;Parent=Msa1150960-mRNA-1
chr7_4 exon 34822667 34822795 34822667 ID=Msa1150960-mRNA-1:exon:8261;Parent=Msa1150960-mRNA-1
chr7_4 exon 34822958 34823624 34822958 ID=Msa1150960-mRNA-1:exon:8262;Parent=Msa1150960-mRNA-1
chr7_4 five_prime_UTR 34820956 34821144 34820956 ID=Msa1150960-mRNA-1:five_prime_utr;Parent=Msa1150960-mRNA-1
chr7_4 CDS 34821145 34821459 34821145 ID=Msa1150960-mRNA-1:cds;Parent=Msa1150960-mRNA-1
chr7_4 CDS 34821566 34821856 34821566 ID=Msa1150960-mRNA-1:cds;Parent=Msa1150960-mRNA-1
chr7_4 CDS 34821938 34822265 34821938 ID=Msa1150960-mRNA-1:cds;Parent=Msa1150960-mRNA-1
chr7_4 CDS 34822667 34822795 34822667 ID=Msa1150960-mRNA-1:cds;Parent=Msa1150960-mRNA-1
chr7_4 CDS 34822958 34823094 34822958 ID=Msa1150960-mRNA-1:cds;Parent=Msa1150960-mRNA-1
chr7_4 three_prime_UTR 34823095 34823624 34823095 ID=Msa1150960-mRNA-1:three_prime_utr;Parent=Msa1150960-mRNA-1
Gene Sequence

>Msa1150960

ATGGCTGAGAATTTGGATGACGGTGAGTTCTGGCTTCCGCCGCAGTTTCTCTCCGACGACGACGGTGACGACACAACCACCACAAAACCGAAACCTTCTTCGCTTTCGCTTTTCAACAATGGTGAAGATCCTCTGCTTTTTCCTTATGGATTCCCTTGCTCTGATCTCAGTTCACCAATCGATTCTCTTGCTGCTTCAAGCGAAACCGAAAGTGACGATGATGAACAACTCTTTGCTGAGTTAACTCGTTGCTTGTCTCGTTCCTCTCTCCATGTTGATTCCAAAGCCTCTGAGAATCATAACAACAATAACTTGGGAGGTTCACCACAATCAACCCTATGTGCTTTTGGAAATGACTCCAAATGCCGCAAAGGTTCATCAAGCCAAGGTAGCCCTAATGGTGTTTGTGAGAATGCTTCATCGAATTCTACTTTGAATCTGTTGCATGCTGCTGCTGATGAAGTTGAAACTTTGAGATTGAATCAACAAACTGTTCCTCAAAGAAATTCTTCTTCCCCTGTTACTTTACCTTCCAAAAACAACCCTGCTGATTTGAGCTTTTTTGCTCCACAATCACTTTCTCATTATCAATTGCAAATTGCTAATTTGCAGAGGATTAGGCAACAACAAATTGCTGAAAGGCTGAACACAATAAGGGCGGGTATGCAGAAGCAAAATGGGGGTGCATTTCAGCAAAGACAAACAAATGGAGGAAGAAATGTTGTTGATGGAAGAAACAACAACAACAACGTTGCTGGTTTAGGTTTGTCATCATCTGCATGGCCAACACTACAACATGCTCAACAAAACCAAATTCAAACTCCACAACCCTACATGAGAGCTGTTTTCCTTGGTAATGGTTATGGTAGCAGTACTGGCACTGGTGTGTTCTTGCCTCGTCCTGTTGAATCAAGAAATTCAACAAAAAAACCAGCTTGTGCAACTGCATTTGTTCCAGCTAGAGTGGCGCATGCCCTTAACCTAAAGCTTGAAGACTATATAGTGGGGTGCCAGCCGCAACAACACTTGCATCGTTTTAATTCCACCTCTAATGTTGAAAATGTAGCTGCTGTTGCACCTCCAAGGCATAGGGGTAATAATGTCAATTCACAAAAGAAGCGCAGCAGCAACAATACCAGCAGGCCTCAGCCAGCAGCAGTGAGCAATGAAATCAAGCTTCCACAGGAGTGGACGTACTGA
Protein sequence

>Msa1150960

MAENLDDGEFWLPPQFLSDDDGDDTTTTKPKPSSLSLFNNGEDPLLFPYGFPCSDLSSPIDSLAASSETESDDDEQLFAELTRCLSRSSLHVDSKASENHNNNNLGGSPQSTLCAFGNDSKCRKGSSSQGSPNGVCENASSNSTLNLLHAAADEVETLRLNQQTVPQRNSSSPVTLPSKNNPADLSFFAPQSLSHYQLQIANLQRIRQQQIAERLNTIRAGMQKQNGGAFQQRQTNGGRNVVDGRNNNNNVAGLGLSSSAWPTLQHAQQNQIQTPQPYMRAVFLGNGYGSSTGTGVFLPRPVESRNSTKKPACATAFVPARVAHALNLKLEDYIVGCQPQQHLHRFNSTSNVENVAAVAPPRHRGNNVNSQKKRSSNNTSRPQPAAVSNEIKLPQEWTY*