AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0337710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337710 MtrunA17_Chr2g0325091 99.070 215 2 0 1 215 1 215 6.54e-159 437
Msa0337710 MtrunA17_Chr4g0046261 80.556 216 39 2 1 213 1 216 1.23e-128 361
Msa0337710 MtrunA17_Chr2g0283131 80.093 216 40 2 1 213 1 216 2.83e-128 360
Msa0337710 MtrunA17_Chr4g0040781 80.556 216 39 2 1 213 1 216 4.16e-128 360
Msa0337710 MtrunA17_Chr2g0315551 75.943 212 48 3 5 214 7 217 7.54e-118 334
Msa0337710 MtrunA17_Chr2g0319951 72.170 212 55 2 5 213 7 217 9.97e-108 308
Msa0337710 MtrunA17_Chr3g0112681 66.981 212 67 2 4 213 5 215 5.12e-102 294
Msa0337710 MtrunA17_Chr2g0276941 65.566 212 70 1 5 213 9 220 2.87e-101 292
Msa0337710 MtrunA17_Chr4g0032201 66.667 213 67 3 4 214 5 215 1.03e-100 290
Msa0337710 MtrunA17_Chr1g0174281 58.065 217 85 2 2 212 6 222 2.57e-89 262
Msa0337710 MtrunA17_Chr6g0482721 59.242 211 83 3 5 213 6 215 1.13e-87 257
Msa0337710 MtrunA17_Chr3g0123821 56.279 215 90 1 2 212 7 221 2.19e-86 254
Msa0337710 MtrunA17_Chr8g0381841 57.339 218 88 2 2 214 8 225 8.49e-86 253
Msa0337710 MtrunA17_Chr7g0231131 55.814 215 90 2 2 212 6 219 1.97e-85 252
Msa0337710 MtrunA17_Chr8g0346421 54.673 214 94 1 4 214 19 232 9.21e-81 240
Msa0337710 MtrunA17_Chr7g0275991 55.869 213 89 3 6 213 15 227 1.29e-80 240
Msa0337710 MtrunA17_Chr8g0392931 56.132 212 89 2 5 213 8 218 2.72e-79 236
Msa0337710 MtrunA17_Chr7g0214241 58.454 207 83 2 10 213 14 220 7.19e-79 235
Msa0337710 MtrunA17_Chr1g0189361 55.660 212 87 2 9 213 12 223 3.63e-77 231
Msa0337710 MtrunA17_Chr8g0351861 60.773 181 70 1 2 181 6 186 1.02e-75 228
Msa0337710 MtrunA17_Chr7g0234201 56.863 204 88 0 10 213 12 215 2.80e-75 226
Msa0337710 MtrunA17_Chr2g0290891 46.009 213 104 2 8 213 3 211 1.15e-61 191
Msa0337710 MtrunA17_Chr6g0458081 43.868 212 110 2 8 213 3 211 1.35e-58 183
Msa0337710 MtrunA17_Chr4g0055651 45.024 211 107 1 4 214 1 202 5.05e-58 182
Msa0337710 MtrunA17_Chr7g0250531 45.024 211 107 1 4 214 1 202 9.03e-57 178
Msa0337710 MtrunA17_Chr8g0335471 46.445 211 105 2 4 214 1 203 1.07e-56 178
Msa0337710 MtrunA17_Chr8g0349711 46.154 208 108 2 10 213 5 212 1.08e-54 174
Msa0337710 MtrunA17_Chr2g0276931 44.444 207 105 2 7 212 5 202 7.32e-54 171
Msa0337710 MtrunA17_Chr1g0182301 42.925 212 111 3 7 214 11 216 5.96e-52 167
Msa0337710 MtrunA17_Chr8g0349681 46.698 212 99 3 8 213 381 584 6.19e-52 176
Msa0337710 MtrunA17_Chr8g0349681 46.988 166 88 0 8 173 172 337 6.74e-46 160
Msa0337710 MtrunA17_Chr8g0349681 50.641 156 77 0 10 165 12 167 9.75e-46 159
Msa0337710 MtrunA17_Chr4g0030911 44.076 211 105 4 7 212 11 213 6.19e-52 166
Msa0337710 MtrunA17_Chr7g0263071 43.458 214 107 4 7 214 11 216 1.33e-51 166
Msa0337710 MtrunA17_Chr1g0182321 42.453 212 112 3 7 214 11 216 3.35e-51 165
Msa0337710 MtrunA17_Chr1g0212521 42.788 208 113 3 7 212 11 214 3.62e-51 164
Msa0337710 MtrunA17_Chr8g0384311 46.190 210 101 7 6 212 8 208 1.33e-49 160
Msa0337710 MtrunA17_Chr2g0327911 41.284 218 108 5 3 212 7 212 4.68e-48 157
Msa0337710 MtrunA17_Chr1g0159481 45.455 209 105 6 7 213 13 214 6.37e-48 156
Msa0337710 MtrunA17_Chr1g0199331 44.720 161 88 1 13 172 33 193 2.44e-45 149
Msa0337710 MtrunA17_Chr3g0130271 43.981 216 104 8 3 212 4 208 2.60e-45 149
Msa0337710 MtrunA17_Chr3g0109721 43.125 160 91 0 13 172 12 171 1.04e-44 147
Msa0337710 MtrunA17_Chr5g0433561 42.500 160 92 0 13 172 12 171 2.24e-43 144
Msa0337710 MtrunA17_Chr7g0265001 42.788 208 111 4 8 213 13 214 7.77e-43 143
Msa0337710 MtrunA17_Chr1g0180291 43.478 207 108 5 8 212 11 210 6.33e-42 141
Msa0337710 MtrunA17_Chr1g0197331 41.981 212 112 5 5 213 9 212 1.15e-41 140
Msa0337710 MtrunA17_Chr3g0123971 33.333 195 112 1 12 206 10 186 1.17e-38 132
Msa0337710 MtrunA17_Chr4g0055081 33.178 214 113 3 12 213 10 205 2.03e-38 132
Msa0337710 MtrunA17_Chr8g0390561 39.375 160 97 0 13 172 11 170 2.06e-38 131
Msa0337710 MtrunA17_Chr5g0400131 34.694 196 109 2 12 206 10 187 4.84e-38 130
Msa0337710 MtrunA17_Chr8g0382221 35.057 174 113 0 12 185 10 183 6.69e-38 130
Msa0337710 MtrunA17_Chr6g0466301 40.000 165 92 3 11 168 8 172 1.81e-37 129
Msa0337710 MtrunA17_Chr6g0465491 40.000 165 92 3 11 168 8 172 1.81e-37 129
Msa0337710 MtrunA17_Chr1g0201121 40.964 166 89 4 11 168 8 172 3.59e-36 126
Msa0337710 MtrunA17_Chr8g0383261 39.759 166 91 4 11 168 12 176 2.62e-35 124
Msa0337710 MtrunA17_Chr4g0034871 34.928 209 116 7 11 211 8 204 1.52e-34 122
Msa0337710 MtrunA17_Chr2g0331691 39.645 169 92 5 11 171 10 176 1.87e-34 122
Msa0337710 MtrunA17_Chr4g0004661 39.506 162 91 3 11 165 64 225 1.04e-32 119
Msa0337710 MtrunA17_Chr6g0466161 36.667 150 88 3 26 168 5 154 8.58e-25 96.7
Msa0337710 MtrunA17_Chr6g0465471 36.667 150 88 3 26 168 5 154 8.86e-25 96.7
Msa0337710 MtrunA17_Chr3g0138121 31.613 155 103 2 12 166 14 165 1.34e-23 94.0
Msa0337710 MtrunA17_Chr3g0138111 31.613 155 103 2 12 166 14 165 1.65e-23 93.6
Msa0337710 MtrunA17_Chr3g0138101 31.613 155 103 2 12 166 14 165 1.65e-23 93.6
Msa0337710 MtrunA17_Chr4g0046211 38.333 120 71 3 11 129 6 123 2.62e-17 75.5
Msa0337710 MtrunA17_Chr4g0037021 31.795 195 114 7 11 190 6 196 2.81e-17 76.6
Msa0337710 MtrunA17_Chr2g0325101 38.333 120 71 3 11 129 6 123 6.00e-17 75.9
Msa0337710 MtrunA17_Chr5g0405791 36.667 120 73 3 11 129 6 123 7.93e-17 75.5
Msa0337710 MtrunA17_Chr6g0466431 35.354 99 55 1 11 109 8 97 1.44e-16 72.4
Msa0337710 MtrunA17_Chr6g0465611 35.354 99 55 1 11 109 8 97 1.44e-16 72.4
Msa0337710 MtrunA17_Chr8g0371501 36.667 120 73 3 11 129 6 123 2.95e-16 73.9
Msa0337710 MtrunA17_Chr3g0117931 30.539 167 102 5 11 165 8 172 4.91e-16 73.6
Msa0337710 MtrunA17_Chr6g0486041 35.537 121 75 3 11 130 6 124 1.22e-15 72.4
Msa0337710 MtrunA17_Chr1g0182951 69.048 42 13 0 120 161 1 42 8.24e-14 63.5
Msa0337710 MtrunA17_Chr1g0178291 31.138 167 102 5 8 171 428 584 3.33e-13 67.8
Msa0337710 MtrunA17_Chr5g0443631 24.260 169 122 2 12 175 105 272 1.91e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337710 AT5G60860.1 80.952 210 39 1 5 213 7 216 1.22e-126 356
Msa0337710 AT3G15060.1 77.725 211 46 1 5 214 7 217 6.18e-123 347
Msa0337710 AT4G18430.1 76.389 216 48 2 1 213 1 216 6.25e-122 345
Msa0337710 AT1G28550.1 76.037 217 48 3 1 213 1 217 1.03e-121 344
Msa0337710 AT2G33870.1 75.943 212 49 2 5 214 7 218 1.18e-119 339
Msa0337710 AT5G45750.1 77.143 210 46 2 5 213 7 215 2.07e-118 335
Msa0337710 AT4G18800.1 76.667 210 45 2 5 213 7 213 1.02e-116 331
Msa0337710 AT1G16920.1 72.986 211 55 2 4 213 6 215 1.10e-112 321
Msa0337710 AT1G06400.1 73.333 210 54 2 5 213 7 215 1.14e-112 321
Msa0337710 AT1G09630.1 65.258 213 71 2 5 215 6 217 2.22e-103 298
Msa0337710 AT1G07410.1 64.151 212 73 2 4 214 5 214 4.72e-100 289
Msa0337710 AT5G59150.1 63.721 215 72 3 4 214 5 217 1.45e-95 278
Msa0337710 AT3G46830.1 63.256 215 73 3 4 214 5 217 2.65e-95 277
Msa0337710 AT3G46830.2 63.256 215 73 3 4 214 5 217 2.65e-95 277
Msa0337710 AT5G47960.1 58.333 216 84 3 5 215 9 223 1.14e-87 258
Msa0337710 AT3G12160.1 57.209 215 87 2 2 212 6 219 1.32e-87 258
Msa0337710 AT4G39990.1 57.619 210 86 1 9 215 15 224 4.91e-85 251
Msa0337710 AT5G65270.1 56.164 219 90 2 2 214 8 226 1.13e-84 251
Msa0337710 AT5G47520.1 57.009 214 88 1 5 214 8 221 1.73e-82 245
Msa0337710 AT1G18200.1 54.260 223 85 3 5 212 7 227 4.56e-80 239
Msa0337710 AT1G73640.1 56.039 207 84 2 5 204 7 213 2.35e-78 235
Msa0337710 AT1G05810.1 57.895 209 84 2 9 214 10 217 2.38e-78 234
Msa0337710 AT3G07410.1 56.944 216 90 2 1 213 1 216 6.94e-78 233
Msa0337710 AT1G05810.2 57.895 209 84 2 9 214 56 263 6.94e-78 234
Msa0337710 AT2G31680.1 55.238 210 91 1 9 215 10 219 1.24e-77 233
Msa0337710 AT2G43130.1 57.692 208 81 2 9 213 10 213 1.35e-77 232
Msa0337710 AT1G01200.1 54.630 216 92 4 4 213 21 236 3.75e-77 232
Msa0337710 AT5G59150.2 57.831 166 64 3 53 214 3 166 1.01e-62 192
Msa0337710 AT4G17170.1 46.009 213 104 2 8 213 3 211 1.02e-61 192
Msa0337710 AT4G35860.1 44.976 209 112 1 8 213 3 211 1.77e-59 186
Msa0337710 AT4G17160.1 45.411 207 106 2 8 212 3 204 3.73e-58 182
Msa0337710 AT1G02130.1 45.972 211 106 3 4 214 1 203 2.10e-56 178
Msa0337710 AT4G17530.1 45.972 211 105 3 4 214 1 202 3.59e-55 175
Msa0337710 AT5G47200.1 44.651 215 102 3 4 214 1 202 7.86e-55 174
Msa0337710 AT3G11730.1 44.231 208 106 3 7 212 4 203 3.12e-53 170
Msa0337710 AT5G03520.1 46.226 212 101 4 7 213 11 214 4.63e-53 170
Msa0337710 AT3G46060.2 43.925 214 106 4 7 214 11 216 2.69e-51 165
Msa0337710 AT3G46060.3 43.925 214 106 4 7 214 11 216 2.69e-51 165
Msa0337710 AT3G46060.1 43.925 214 106 4 7 214 11 216 2.69e-51 165
Msa0337710 AT3G53610.2 42.991 214 108 4 7 214 11 216 7.78e-51 164
Msa0337710 AT3G53610.3 42.991 214 108 4 7 214 11 216 7.78e-51 164
Msa0337710 AT3G53610.1 42.991 214 108 4 7 214 11 216 7.78e-51 164
Msa0337710 AT3G09900.1 44.393 214 104 4 7 213 11 216 8.72e-51 164
Msa0337710 AT5G59840.1 42.991 214 108 4 7 214 11 216 4.37e-50 162
Msa0337710 AT5G45130.1 40.299 201 108 4 13 213 12 200 7.80e-47 153
Msa0337710 AT1G43890.3 47.701 174 88 3 6 177 8 180 3.93e-46 152
Msa0337710 AT1G43890.1 47.701 174 88 3 6 177 8 180 3.93e-46 152
Msa0337710 AT1G43890.2 47.701 174 88 3 6 177 8 180 3.93e-46 152
Msa0337710 AT3G54840.1 47.134 157 82 1 13 168 35 191 9.37e-46 150
Msa0337710 AT3G54840.3 46.795 156 82 1 14 168 2 157 2.65e-45 148
Msa0337710 AT5G45130.2 39.698 199 108 4 15 213 22 208 4.14e-45 149
Msa0337710 AT3G54840.2 47.403 154 80 1 13 165 35 188 5.52e-45 148
Msa0337710 AT4G19640.1 42.331 163 94 0 13 175 12 174 6.78e-45 148
Msa0337710 AT5G03530.2 43.750 208 108 5 8 213 10 210 4.59e-44 147
Msa0337710 AT5G03530.1 43.750 208 108 5 8 213 10 210 4.59e-44 147
Msa0337710 AT4G35860.2 41.463 164 93 1 53 213 2 165 1.56e-40 136
Msa0337710 AT5G64990.1 37.267 161 101 0 12 172 8 168 3.52e-38 131
Msa0337710 AT2G22290.1 33.168 202 130 2 12 213 10 206 7.05e-38 130
Msa0337710 AT2G44610.1 33.333 216 112 3 12 213 10 207 7.09e-38 130
Msa0337710 AT1G22740.1 42.515 167 85 5 11 168 8 172 8.01e-38 130
Msa0337710 AT5G03520.2 42.775 173 86 4 46 213 40 204 1.08e-37 130
Msa0337710 AT5G10260.1 36.943 157 99 0 12 168 10 166 1.24e-36 127
Msa0337710 AT4G39890.1 36.364 165 104 1 12 175 10 174 1.93e-36 127
Msa0337710 AT4G09720.1 39.394 165 93 3 11 168 8 172 5.92e-36 125
Msa0337710 AT5G64990.2 35.714 168 101 1 12 172 8 175 1.90e-35 125
Msa0337710 AT3G09910.3 42.781 187 100 4 8 192 10 191 2.92e-35 124
Msa0337710 AT3G09910.1 42.781 187 100 4 8 192 10 191 2.92e-35 124
Msa0337710 AT3G09910.2 42.781 187 100 4 8 192 10 191 2.92e-35 124
Msa0337710 AT2G21880.1 40.000 165 92 3 11 168 9 173 3.00e-35 124
Msa0337710 AT4G19640.2 43.077 130 74 0 13 142 12 141 5.34e-35 121
Msa0337710 AT5G46025.1 57.547 106 44 1 73 177 6 111 7.01e-35 120
Msa0337710 AT1G52280.1 40.237 169 91 5 11 171 8 174 7.25e-35 123
Msa0337710 AT4G19640.3 42.748 131 75 0 13 143 12 142 1.56e-34 122
Msa0337710 AT4G09720.4 38.824 170 92 4 11 168 8 177 2.42e-34 122
Msa0337710 AT3G18820.1 39.506 162 91 3 11 165 8 169 1.12e-33 120
Msa0337710 AT3G09910.5 43.114 167 92 3 8 172 10 175 1.34e-33 119
Msa0337710 AT3G09910.4 43.114 167 92 3 8 172 10 175 1.34e-33 119
Msa0337710 AT4G09720.3 37.500 176 92 4 11 168 8 183 2.58e-33 119
Msa0337710 AT3G16100.1 39.264 163 90 4 11 165 8 169 4.29e-33 118
Msa0337710 AT1G49300.2 37.725 167 97 3 11 170 8 174 1.29e-32 117
Msa0337710 AT1G49300.1 37.725 167 97 3 11 170 8 174 1.29e-32 117
Msa0337710 AT5G39620.1 39.024 164 93 3 12 168 6 169 2.31e-32 116
Msa0337710 AT2G21880.4 37.838 148 85 3 28 168 1 148 1.50e-26 101
Msa0337710 AT2G21880.3 37.838 148 85 3 28 168 1 148 1.50e-26 101
Msa0337710 AT4G09720.6 39.130 138 77 3 38 168 1 138 2.06e-25 97.8
Msa0337710 AT4G09720.5 39.130 138 77 3 38 168 1 138 2.06e-25 97.8
Msa0337710 AT4G09720.2 39.130 138 77 3 38 168 1 138 2.06e-25 97.8
Msa0337710 AT2G21880.2 35.152 165 92 4 11 168 9 165 2.75e-25 98.2
Msa0337710 AT5G55080.1 31.056 161 108 2 12 172 14 171 1.77e-23 94.0
Msa0337710 AT5G20020.1 31.613 155 103 2 12 166 14 165 1.95e-23 94.0
Msa0337710 AT5G20010.1 31.613 155 103 2 12 166 14 165 2.23e-23 94.0
Msa0337710 AT5G55190.1 31.613 155 103 2 12 166 14 165 2.35e-23 93.6
Msa0337710 AT2G44690.1 32.778 180 107 5 11 178 18 195 1.28e-19 83.6
Msa0337710 AT4G08190.1 50.704 71 32 1 95 165 60 127 5.15e-18 77.4
Msa0337710 AT4G35020.1 34.000 150 89 4 11 159 6 146 3.95e-17 76.6
Msa0337710 AT4G35020.2 34.000 150 89 4 11 159 6 146 3.95e-17 76.6
Msa0337710 AT4G35020.3 34.000 150 89 4 11 159 6 146 3.95e-17 76.6
Msa0337710 AT1G20090.1 37.500 120 72 3 11 129 5 122 1.64e-16 75.1
Msa0337710 AT1G75840.1 36.667 120 73 3 11 129 6 123 2.64e-16 74.3
Msa0337710 AT5G45970.1 37.500 120 72 3 11 129 6 123 4.57e-16 73.9
Msa0337710 AT2G17800.2 36.667 120 73 3 11 129 6 123 5.11e-16 73.6
Msa0337710 AT2G17800.1 36.667 120 73 3 11 129 6 123 5.11e-16 73.6
Msa0337710 AT3G51300.1 37.288 118 71 3 13 129 8 123 5.61e-16 73.6
Msa0337710 AT4G35950.1 36.667 120 73 3 11 129 6 123 5.85e-16 73.6
Msa0337710 AT3G48040.1 31.138 167 101 5 11 165 8 172 7.35e-16 73.6
Msa0337710 AT4G28950.1 35.833 120 74 3 11 129 6 123 1.02e-15 73.2
Msa0337710 AT5G62880.1 30.899 178 105 6 11 172 8 183 1.79e-15 72.8
Msa0337710 AT3G48040.2 35.833 120 74 3 11 129 8 125 3.83e-15 70.9
Msa0337710 AT5G62880.2 35.000 120 75 3 11 129 8 125 8.14e-15 70.5
Msa0337710 AT4G35020.4 35.246 122 76 3 13 133 8 127 4.68e-14 67.4
Msa0337710 AT4G35020.5 35.246 122 76 3 13 133 8 127 4.68e-14 67.4

Find 50 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTAAATTCTCATCATTT+TGG 0.200341 2_4:+57793790 Msa0337710:three_prime_UTR
CATTATTGTTGTTACAATTC+AGG 0.242895 2_4:+57793931 Msa0337710:three_prime_UTR
TTCAACACCGATGGTGGATT+TGG 0.251856 2_4:-57792161 None:intergenic
CTGAATTGTAACAACAATAA+TGG 0.327085 2_4:-57793930 None:intergenic
ATCATCTTTACCAATATTGA+TGG 0.346184 2_4:-57793677 None:intergenic
GCTTGTTAAGGCTCAGATTT+GGG 0.349305 2_4:+57792216 Msa0337710:CDS
TGGAGCTGTTGGCGCATTGC+TGG 0.351844 2_4:+57793339 Msa0337710:CDS
AGCTTGTTAAGGCTCAGATT+TGG 0.356509 2_4:+57792215 Msa0337710:CDS
GAAAGAAAACATATATTTCA+TGG 0.359463 2_4:+57793525 Msa0337710:CDS
AAAATTCGATGGTAAAATAA+AGG 0.371266 2_4:-57793847 None:intergenic
TTTCGGTTGCTGAGATATAT+AGG 0.409081 2_4:+57793886 Msa0337710:three_prime_UTR
GATTCTTGATAAGCTTGTTA+AGG 0.421134 2_4:+57792204 Msa0337710:CDS
GAGAGACGTGTACTCTTTGA+AGG 0.424290 2_4:-57791952 None:intergenic
AAGGGAGAGACCATCAATAT+TGG 0.434305 2_4:+57793667 Msa0337710:CDS
CACTAGAAATGAATTCAACT+TGG 0.437767 2_4:+57792135 Msa0337710:CDS
TGGCTCTGCGTCAGTGCCTA+AGG 0.440225 2_4:+57793648 Msa0337710:CDS
AATTTGCTACTCGCAGTGTC+AGG 0.455604 2_4:+57792182 Msa0337710:CDS
TATGTTGTAATCATGCTTGT+AGG 0.477521 2_4:+57793439 Msa0337710:CDS
AAATATTGAGAGGTGGTTGA+AGG 0.482020 2_4:+57793393 Msa0337710:CDS
TTGATCGGTGATTCTGGTGT+TGG 0.493431 2_4:+57792088 Msa0337710:CDS
AGTGAATCTAGATAAAAGAT+TGG 0.496154 2_4:-57792116 None:intergenic
ATATTGATGGTCTCTCCCTT+AGG 0.505876 2_4:-57793664 None:intergenic
GTAGGAGACTGTTACAGAAA+AGG 0.507063 2_4:-57791921 None:intergenic
TTCTTTCTCAGCAAATGTGG+TGG 0.512980 2_4:-57793509 None:intergenic
AGAGATGTATACATTAGAAG+AGG 0.513980 2_4:+57793753 Msa0337710:three_prime_UTR
AAGACTCTTGAGAAAGAGAA+TGG 0.519287 2_4:+57793628 Msa0337710:CDS
AGCAAATTCAACACCGATGG+TGG 0.523464 2_4:-57792167 None:intergenic
GTTTCAGATGTTAAGAAGAG+TGG 0.531135 2_4:+57793700 Msa0337710:CDS
GGGATACTGCTGGTCAAGAA+AGG 0.539209 2_4:+57792236 Msa0337710:CDS
ATACGATTACTTGTTCAAGT+TGG 0.541653 2_4:+57792063 Msa0337710:CDS
TGCACTAGAGTCGCTGAATG+TGG 0.555293 2_4:+57793555 Msa0337710:CDS
GCATACTACCGTGGAGCTGT+TGG 0.560757 2_4:+57793328 Msa0337710:CDS
GAGCGTGAATTCTATTCATA+CGG 0.567954 2_4:+57791895 None:intergenic
GAATGTGGACAATGCTTTCG+TGG 0.568957 2_4:+57793570 Msa0337710:CDS
GACGTGTACTCTTTGAAGGA+AGG 0.571566 2_4:-57791948 None:intergenic
GCTCAGATTTGGGATACTGC+TGG 0.571910 2_4:+57792226 Msa0337710:CDS
TACAAGCATGATTACAACAT+AGG 0.576438 2_4:-57793437 None:intergenic
GGCTCTGCGTCAGTGCCTAA+GGG 0.585884 2_4:+57793649 Msa0337710:CDS
ACACTTGCAAGCTGTGTCAA+CGG 0.600216 2_4:+57793480 Msa0337710:CDS
TGCTGGTGTACGACTCAACA+AGG 0.604227 2_4:+57793356 Msa0337710:CDS
CAACCGAAAACACATTACAT+AGG 0.609931 2_4:-57793872 None:intergenic
TTACATAGGACAAAATTCGA+TGG 0.629469 2_4:-57793858 None:intergenic
AGTAGCAAATTCAACACCGA+TGG 0.634065 2_4:-57792170 None:intergenic
TTGGAATCCAAATCCACCAT+CGG 0.638269 2_4:+57792154 Msa0337710:CDS
CATTTGAAAATATTGAGAGG+TGG 0.642162 2_4:+57793386 Msa0337710:CDS
GGAGACTGTTACAGAAAAGG+AGG 0.650383 2_4:-57791918 None:intergenic
GCAATGCGCCAACAGCTCCA+CGG 0.668645 2_4:-57793336 None:intergenic
TCTTTGAAGGAAGGAAACGT+AGG 0.691114 2_4:-57791939 None:intergenic
TGACATTTGAAAATATTGAG+AGG 0.704546 2_4:+57793383 Msa0337710:CDS
ATCACAAGTGCATACTACCG+TGG 0.721157 2_4:+57793319 Msa0337710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAAAAACATTTCAAA+TGG - chr2_4:57793819-57793838 None:intergenic 10.0%
!! AAATATAAAAACATTTCAAA+TGG - chr2_4:57793819-57793838 None:intergenic 10.0%
!!! TACTCTTTCATTTTTAATGT+CGG + chr2_4:57792586-57792605 Msa0337710:intron 20.0%
!! TTCTTAATATTGTAGAGAAT+TGG + chr2_4:57792675-57792694 Msa0337710:intron 20.0%
!! TCTTAATATTGTAGAGAATT+GGG + chr2_4:57792676-57792695 Msa0337710:intron 20.0%
!! ATATTGGAAGAAATAAACAA+TGG - chr2_4:57792751-57792770 None:intergenic 20.0%
!! TGAAAAAACTAAATGGATAT+TGG - chr2_4:57792767-57792786 None:intergenic 20.0%
!!! CAAACTTTGAAAAAACTAAA+TGG - chr2_4:57792774-57792793 None:intergenic 20.0%
!!! AAATTGAACTCTTATTTTAG+AGG - chr2_4:57793068-57793087 None:intergenic 20.0%
!! GAAAGAAAACATATATTTCA+TGG + chr2_4:57793525-57793544 Msa0337710:CDS 20.0%
!! AAAATTCGATGGTAAAATAA+AGG - chr2_4:57793850-57793869 None:intergenic 20.0%
!!! TACTCTTTCATTTTTAATGT+CGG + chr2_4:57792586-57792605 Msa0337710:intron 20.0%
!! TTCTTAATATTGTAGAGAAT+TGG + chr2_4:57792675-57792694 Msa0337710:intron 20.0%
!! TCTTAATATTGTAGAGAATT+GGG + chr2_4:57792676-57792695 Msa0337710:intron 20.0%
!! ATATTGGAAGAAATAAACAA+TGG - chr2_4:57792751-57792770 None:intergenic 20.0%
!! TGAAAAAACTAAATGGATAT+TGG - chr2_4:57792767-57792786 None:intergenic 20.0%
!!! CAAACTTTGAAAAAACTAAA+TGG - chr2_4:57792774-57792793 None:intergenic 20.0%
!!! AAATTGAACTCTTATTTTAG+AGG - chr2_4:57793068-57793087 None:intergenic 20.0%
!! GAAAGAAAACATATATTTCA+TGG + chr2_4:57793525-57793544 Msa0337710:CDS 20.0%
!! AAAATTCGATGGTAAAATAA+AGG - chr2_4:57793850-57793869 None:intergenic 20.0%
! GATCGAAAAATGAATGAAAA+GGG - chr2_4:57791996-57792015 None:intergenic 25.0%
! AGATCGAAAAATGAATGAAA+AGG - chr2_4:57791997-57792016 None:intergenic 25.0%
!! TTTTTCATCATCATCATCAA+TGG + chr2_4:57792024-57792043 Msa0337710:exon 25.0%
!! TTTTCATCATCATCATCAAT+GGG + chr2_4:57792025-57792044 Msa0337710:exon 25.0%
! AGTGAATCTAGATAAAAGAT+TGG - chr2_4:57792119-57792138 None:intergenic 25.0%
! AGAATGAAATTGAAGAGAAA+AGG - chr2_4:57792267-57792286 None:intergenic 25.0%
!!! GCAAAAAGTGTTTTTTTTTG+TGG + chr2_4:57792321-57792340 Msa0337710:intron 25.0%
!!! AGTGTTTTTTTTTGTGGTTT+TGG + chr2_4:57792327-57792346 Msa0337710:intron 25.0%
!!! GTGTTTTTTTTTGTGGTTTT+GGG + chr2_4:57792328-57792347 Msa0337710:intron 25.0%
! GATTGATGAATTGAATTGAA+TGG + chr2_4:57792428-57792447 Msa0337710:intron 25.0%
!!! TACTTTTTTGCTTTTGATCT+TGG + chr2_4:57792472-57792491 Msa0337710:intron 25.0%
!!! TGTATCTTAGAATTTGTTGT+TGG + chr2_4:57792526-57792545 Msa0337710:intron 25.0%
!!! GTATCTTAGAATTTGTTGTT+GGG + chr2_4:57792527-57792546 Msa0337710:intron 25.0%
!!! ACTCTTTCATTTTTAATGTC+GGG + chr2_4:57792587-57792606 Msa0337710:intron 25.0%
! GTTCAATCTGTTGTTAAATA+AGG + chr2_4:57792610-57792629 Msa0337710:intron 25.0%
! ATGATCACAAAAACTATAGA+TGG + chr2_4:57792715-57792734 Msa0337710:intron 25.0%
!! CAATTTGTTCAATATATCGA+AGG + chr2_4:57792794-57792813 Msa0337710:intron 25.0%
! AAAACGAGTTTACAAGATTT+AGG - chr2_4:57793154-57793173 None:intergenic 25.0%
! AGACAGAAAAATTCTCTAAA+TGG - chr2_4:57793196-57793215 None:intergenic 25.0%
! TGACATTTGAAAATATTGAG+AGG + chr2_4:57793383-57793402 Msa0337710:CDS 25.0%
! ATCATCTTTACCAATATTGA+TGG - chr2_4:57793680-57793699 None:intergenic 25.0%
!! AGAGTAAATTCTCATCATTT+TGG + chr2_4:57793790-57793809 Msa0337710:three_prime_UTR 25.0%
!! GAGTAAATTCTCATCATTTT+GGG + chr2_4:57793791-57793810 Msa0337710:three_prime_UTR 25.0%
! CTGAATTGTAACAACAATAA+TGG - chr2_4:57793933-57793952 None:intergenic 25.0%
!! CATTATTGTTGTTACAATTC+AGG + chr2_4:57793931-57793950 Msa0337710:three_prime_UTR 25.0%
! GATCGAAAAATGAATGAAAA+GGG - chr2_4:57791996-57792015 None:intergenic 25.0%
! AGATCGAAAAATGAATGAAA+AGG - chr2_4:57791997-57792016 None:intergenic 25.0%
!! TTTTTCATCATCATCATCAA+TGG + chr2_4:57792024-57792043 Msa0337710:exon 25.0%
!! TTTTCATCATCATCATCAAT+GGG + chr2_4:57792025-57792044 Msa0337710:exon 25.0%
! AGTGAATCTAGATAAAAGAT+TGG - chr2_4:57792119-57792138 None:intergenic 25.0%
! AGAATGAAATTGAAGAGAAA+AGG - chr2_4:57792267-57792286 None:intergenic 25.0%
!!! GCAAAAAGTGTTTTTTTTTG+TGG + chr2_4:57792321-57792340 Msa0337710:intron 25.0%
!!! AGTGTTTTTTTTTGTGGTTT+TGG + chr2_4:57792327-57792346 Msa0337710:intron 25.0%
!!! GTGTTTTTTTTTGTGGTTTT+GGG + chr2_4:57792328-57792347 Msa0337710:intron 25.0%
! GATTGATGAATTGAATTGAA+TGG + chr2_4:57792428-57792447 Msa0337710:intron 25.0%
!!! TACTTTTTTGCTTTTGATCT+TGG + chr2_4:57792472-57792491 Msa0337710:intron 25.0%
!!! TGTATCTTAGAATTTGTTGT+TGG + chr2_4:57792526-57792545 Msa0337710:intron 25.0%
!!! GTATCTTAGAATTTGTTGTT+GGG + chr2_4:57792527-57792546 Msa0337710:intron 25.0%
!!! ACTCTTTCATTTTTAATGTC+GGG + chr2_4:57792587-57792606 Msa0337710:intron 25.0%
! GTTCAATCTGTTGTTAAATA+AGG + chr2_4:57792610-57792629 Msa0337710:intron 25.0%
! ATGATCACAAAAACTATAGA+TGG + chr2_4:57792715-57792734 Msa0337710:intron 25.0%
!! CAATTTGTTCAATATATCGA+AGG + chr2_4:57792794-57792813 Msa0337710:intron 25.0%
! AAAACGAGTTTACAAGATTT+AGG - chr2_4:57793154-57793173 None:intergenic 25.0%
! AGACAGAAAAATTCTCTAAA+TGG - chr2_4:57793196-57793215 None:intergenic 25.0%
! TGACATTTGAAAATATTGAG+AGG + chr2_4:57793383-57793402 Msa0337710:CDS 25.0%
! ATCATCTTTACCAATATTGA+TGG - chr2_4:57793680-57793699 None:intergenic 25.0%
!! AGAGTAAATTCTCATCATTT+TGG + chr2_4:57793790-57793809 Msa0337710:three_prime_UTR 25.0%
!! GAGTAAATTCTCATCATTTT+GGG + chr2_4:57793791-57793810 Msa0337710:three_prime_UTR 25.0%
! CTGAATTGTAACAACAATAA+TGG - chr2_4:57793933-57793952 None:intergenic 25.0%
!! CATTATTGTTGTTACAATTC+AGG + chr2_4:57793931-57793950 Msa0337710:three_prime_UTR 25.0%
ATACGATTACTTGTTCAAGT+TGG + chr2_4:57792063-57792082 Msa0337710:CDS 30.0%
!!! TTGTTCAAGTTGGTTTTGAT+CGG + chr2_4:57792073-57792092 Msa0337710:CDS 30.0%
CACTAGAAATGAATTCAACT+TGG + chr2_4:57792135-57792154 Msa0337710:CDS 30.0%
!! GATTCTTGATAAGCTTGTTA+AGG + chr2_4:57792204-57792223 Msa0337710:CDS 30.0%
!! GGGTTTTGATCTTAATGTTT+AGG + chr2_4:57792348-57792367 Msa0337710:intron 30.0%
!! GACAGTGATAATTTGTTGTT+GGG + chr2_4:57792370-57792389 Msa0337710:intron 30.0%
AATGGAGTGAAAATGCAAAA+GGG + chr2_4:57792446-57792465 Msa0337710:intron 30.0%
!! CTCTTTCATTTTTAATGTCG+GGG + chr2_4:57792588-57792607 Msa0337710:intron 30.0%
GGAAGAAATAAACAATGGTT+AGG - chr2_4:57792746-57792765 None:intergenic 30.0%
!! GACTTTTAGAAGAGAAACTA+TGG - chr2_4:57792918-57792937 None:intergenic 30.0%
AGGAAAAAGTAAGTGCATAT+TGG - chr2_4:57793048-57793067 None:intergenic 30.0%
! AAATGGAACTCTCATTTTAG+AGG - chr2_4:57793179-57793198 None:intergenic 30.0%
AATAAGAGACTACAAGATTC+AGG - chr2_4:57793271-57793290 None:intergenic 30.0%
CATTTGAAAATATTGAGAGG+TGG + chr2_4:57793386-57793405 Msa0337710:CDS 30.0%
TACAAGCATGATTACAACAT+AGG - chr2_4:57793440-57793459 None:intergenic 30.0%
TATGTTGTAATCATGCTTGT+AGG + chr2_4:57793439-57793458 Msa0337710:CDS 30.0%
AGAGATGTATACATTAGAAG+AGG + chr2_4:57793753-57793772 Msa0337710:three_prime_UTR 30.0%
TTACATAGGACAAAATTCGA+TGG - chr2_4:57793861-57793880 None:intergenic 30.0%
! TGTCCTATGTAATGTGTTTT+CGG + chr2_4:57793869-57793888 Msa0337710:three_prime_UTR 30.0%
ATACGATTACTTGTTCAAGT+TGG + chr2_4:57792063-57792082 Msa0337710:CDS 30.0%
!!! TTGTTCAAGTTGGTTTTGAT+CGG + chr2_4:57792073-57792092 Msa0337710:CDS 30.0%
CACTAGAAATGAATTCAACT+TGG + chr2_4:57792135-57792154 Msa0337710:CDS 30.0%
!! GATTCTTGATAAGCTTGTTA+AGG + chr2_4:57792204-57792223 Msa0337710:CDS 30.0%
!! GGGTTTTGATCTTAATGTTT+AGG + chr2_4:57792348-57792367 Msa0337710:intron 30.0%
!! GACAGTGATAATTTGTTGTT+GGG + chr2_4:57792370-57792389 Msa0337710:intron 30.0%
AATGGAGTGAAAATGCAAAA+GGG + chr2_4:57792446-57792465 Msa0337710:intron 30.0%
!! CTCTTTCATTTTTAATGTCG+GGG + chr2_4:57792588-57792607 Msa0337710:intron 30.0%
GGAAGAAATAAACAATGGTT+AGG - chr2_4:57792746-57792765 None:intergenic 30.0%
!! GACTTTTAGAAGAGAAACTA+TGG - chr2_4:57792918-57792937 None:intergenic 30.0%
AGGAAAAAGTAAGTGCATAT+TGG - chr2_4:57793048-57793067 None:intergenic 30.0%
! AAATGGAACTCTCATTTTAG+AGG - chr2_4:57793179-57793198 None:intergenic 30.0%
AATAAGAGACTACAAGATTC+AGG - chr2_4:57793271-57793290 None:intergenic 30.0%
CATTTGAAAATATTGAGAGG+TGG + chr2_4:57793386-57793405 Msa0337710:CDS 30.0%
TACAAGCATGATTACAACAT+AGG - chr2_4:57793440-57793459 None:intergenic 30.0%
TATGTTGTAATCATGCTTGT+AGG + chr2_4:57793439-57793458 Msa0337710:CDS 30.0%
AGAGATGTATACATTAGAAG+AGG + chr2_4:57793753-57793772 Msa0337710:three_prime_UTR 30.0%
TTACATAGGACAAAATTCGA+TGG - chr2_4:57793861-57793880 None:intergenic 30.0%
! TGTCCTATGTAATGTGTTTT+CGG + chr2_4:57793869-57793888 Msa0337710:three_prime_UTR 30.0%
!! GGACAGTGATAATTTGTTGT+TGG + chr2_4:57792369-57792388 Msa0337710:intron 35.0%
GAATGGAGTGAAAATGCAAA+AGG + chr2_4:57792445-57792464 Msa0337710:intron 35.0%
AAGACACTGATCATAAACAG+AGG + chr2_4:57792500-57792519 Msa0337710:intron 35.0%
!! AATTTGTTGTTGGGTAGAGA+AGG + chr2_4:57792379-57792398 Msa0337710:intron 35.0%
ATGATTGTTCCAATTACCCT+TGG + chr2_4:57792830-57792849 Msa0337710:intron 35.0%
!! AGTTCTAGAAGAGAATCCAA+GGG - chr2_4:57792849-57792868 None:intergenic 35.0%
GAAGAGAAACTATGGCTAAT+TGG - chr2_4:57792910-57792929 None:intergenic 35.0%
AAGATGTCTTACTGCATGTT+TGG + chr2_4:57792983-57793002 Msa0337710:intron 35.0%
GTAAGTGCATATTGGTATCA+CGG - chr2_4:57793040-57793059 None:intergenic 35.0%
AAATATTGAGAGGTGGTTGA+AGG + chr2_4:57793393-57793412 Msa0337710:CDS 35.0%
! GTTTTCTTTCTCAGCAAATG+TGG - chr2_4:57793515-57793534 None:intergenic 35.0%
AAGACTCTTGAGAAAGAGAA+TGG + chr2_4:57793628-57793647 Msa0337710:CDS 35.0%
GTTTCAGATGTTAAGAAGAG+TGG + chr2_4:57793700-57793719 Msa0337710:CDS 35.0%
CAACCGAAAACACATTACAT+AGG - chr2_4:57793875-57793894 None:intergenic 35.0%
TTTCGGTTGCTGAGATATAT+AGG + chr2_4:57793886-57793905 Msa0337710:three_prime_UTR 35.0%
!! GGACAGTGATAATTTGTTGT+TGG + chr2_4:57792369-57792388 Msa0337710:intron 35.0%
GAATGGAGTGAAAATGCAAA+AGG + chr2_4:57792445-57792464 Msa0337710:intron 35.0%
AAGACACTGATCATAAACAG+AGG + chr2_4:57792500-57792519 Msa0337710:intron 35.0%
!! AATTTGTTGTTGGGTAGAGA+AGG + chr2_4:57792379-57792398 Msa0337710:intron 35.0%
ATGATTGTTCCAATTACCCT+TGG + chr2_4:57792830-57792849 Msa0337710:intron 35.0%
!! AGTTCTAGAAGAGAATCCAA+GGG - chr2_4:57792849-57792868 None:intergenic 35.0%
GAAGAGAAACTATGGCTAAT+TGG - chr2_4:57792910-57792929 None:intergenic 35.0%
AAGATGTCTTACTGCATGTT+TGG + chr2_4:57792983-57793002 Msa0337710:intron 35.0%
GTAAGTGCATATTGGTATCA+CGG - chr2_4:57793040-57793059 None:intergenic 35.0%
AAATATTGAGAGGTGGTTGA+AGG + chr2_4:57793393-57793412 Msa0337710:CDS 35.0%
! GTTTTCTTTCTCAGCAAATG+TGG - chr2_4:57793515-57793534 None:intergenic 35.0%
AAGACTCTTGAGAAAGAGAA+TGG + chr2_4:57793628-57793647 Msa0337710:CDS 35.0%
GTTTCAGATGTTAAGAAGAG+TGG + chr2_4:57793700-57793719 Msa0337710:CDS 35.0%
CAACCGAAAACACATTACAT+AGG - chr2_4:57793875-57793894 None:intergenic 35.0%
TTTCGGTTGCTGAGATATAT+AGG + chr2_4:57793886-57793905 Msa0337710:three_prime_UTR 35.0%
GTAGGAGACTGTTACAGAAA+AGG - chr2_4:57791924-57791943 None:intergenic 40.0%
! TCTTTGAAGGAAGGAAACGT+AGG - chr2_4:57791942-57791961 None:intergenic 40.0%
!!! TTGGTTTTGATCGGTGATTC+TGG + chr2_4:57792082-57792101 Msa0337710:CDS 40.0%
TTGGAATCCAAATCCACCAT+CGG + chr2_4:57792154-57792173 Msa0337710:CDS 40.0%
AGTAGCAAATTCAACACCGA+TGG - chr2_4:57792173-57792192 None:intergenic 40.0%
!! AGCTTGTTAAGGCTCAGATT+TGG + chr2_4:57792215-57792234 Msa0337710:CDS 40.0%
! GCTTGTTAAGGCTCAGATTT+GGG + chr2_4:57792216-57792235 Msa0337710:CDS 40.0%
GTTGGGTAGAGAATGAATTG+AGG + chr2_4:57792387-57792406 Msa0337710:intron 40.0%
TGTTGGGTAGAGAAGGAATT+TGG + chr2_4:57792543-57792562 Msa0337710:intron 40.0%
GTTGGGTAGAGAAGGAATTT+GGG + chr2_4:57792544-57792563 Msa0337710:intron 40.0%
GAAGAGAATCCAAGGGTAAT+TGG - chr2_4:57792842-57792861 None:intergenic 40.0%
!! GAGTTCTAGAAGAGAATCCA+AGG - chr2_4:57792850-57792869 None:intergenic 40.0%
TTCTTTCTCAGCAAATGTGG+TGG - chr2_4:57793512-57793531 None:intergenic 40.0%
! ATATTGATGGTCTCTCCCTT+AGG - chr2_4:57793667-57793686 None:intergenic 40.0%
AAGGGAGAGACCATCAATAT+TGG + chr2_4:57793667-57793686 Msa0337710:CDS 40.0%
GTAGGAGACTGTTACAGAAA+AGG - chr2_4:57791924-57791943 None:intergenic 40.0%
! TCTTTGAAGGAAGGAAACGT+AGG - chr2_4:57791942-57791961 None:intergenic 40.0%
!!! TTGGTTTTGATCGGTGATTC+TGG + chr2_4:57792082-57792101 Msa0337710:CDS 40.0%
TTGGAATCCAAATCCACCAT+CGG + chr2_4:57792154-57792173 Msa0337710:CDS 40.0%
AGTAGCAAATTCAACACCGA+TGG - chr2_4:57792173-57792192 None:intergenic 40.0%
!! AGCTTGTTAAGGCTCAGATT+TGG + chr2_4:57792215-57792234 Msa0337710:CDS 40.0%
! GCTTGTTAAGGCTCAGATTT+GGG + chr2_4:57792216-57792235 Msa0337710:CDS 40.0%
GTTGGGTAGAGAATGAATTG+AGG + chr2_4:57792387-57792406 Msa0337710:intron 40.0%
TGTTGGGTAGAGAAGGAATT+TGG + chr2_4:57792543-57792562 Msa0337710:intron 40.0%
GTTGGGTAGAGAAGGAATTT+GGG + chr2_4:57792544-57792563 Msa0337710:intron 40.0%
GAAGAGAATCCAAGGGTAAT+TGG - chr2_4:57792842-57792861 None:intergenic 40.0%
!! GAGTTCTAGAAGAGAATCCA+AGG - chr2_4:57792850-57792869 None:intergenic 40.0%
TTCTTTCTCAGCAAATGTGG+TGG - chr2_4:57793512-57793531 None:intergenic 40.0%
! ATATTGATGGTCTCTCCCTT+AGG - chr2_4:57793667-57793686 None:intergenic 40.0%
AAGGGAGAGACCATCAATAT+TGG + chr2_4:57793667-57793686 Msa0337710:CDS 40.0%
GGAGACTGTTACAGAAAAGG+AGG - chr2_4:57791921-57791940 None:intergenic 45.0%
! GACGTGTACTCTTTGAAGGA+AGG - chr2_4:57791951-57791970 None:intergenic 45.0%
GAGAGACGTGTACTCTTTGA+AGG - chr2_4:57791955-57791974 None:intergenic 45.0%
TTGATCGGTGATTCTGGTGT+TGG + chr2_4:57792088-57792107 Msa0337710:CDS 45.0%
!! TTCAACACCGATGGTGGATT+TGG - chr2_4:57792164-57792183 None:intergenic 45.0%
AGCAAATTCAACACCGATGG+TGG - chr2_4:57792170-57792189 None:intergenic 45.0%
!! AATTTGCTACTCGCAGTGTC+AGG + chr2_4:57792182-57792201 Msa0337710:CDS 45.0%
ATCACAAGTGCATACTACCG+TGG + chr2_4:57793319-57793338 Msa0337710:CDS 45.0%
ACACTTGCAAGCTGTGTCAA+CGG + chr2_4:57793480-57793499 Msa0337710:CDS 45.0%
GAATGTGGACAATGCTTTCG+TGG + chr2_4:57793570-57793589 Msa0337710:CDS 45.0%
GGAGACTGTTACAGAAAAGG+AGG - chr2_4:57791921-57791940 None:intergenic 45.0%
! GACGTGTACTCTTTGAAGGA+AGG - chr2_4:57791951-57791970 None:intergenic 45.0%
GAGAGACGTGTACTCTTTGA+AGG - chr2_4:57791955-57791974 None:intergenic 45.0%
TTGATCGGTGATTCTGGTGT+TGG + chr2_4:57792088-57792107 Msa0337710:CDS 45.0%
!! TTCAACACCGATGGTGGATT+TGG - chr2_4:57792164-57792183 None:intergenic 45.0%
AGCAAATTCAACACCGATGG+TGG - chr2_4:57792170-57792189 None:intergenic 45.0%
!! AATTTGCTACTCGCAGTGTC+AGG + chr2_4:57792182-57792201 Msa0337710:CDS 45.0%
ATCACAAGTGCATACTACCG+TGG + chr2_4:57793319-57793338 Msa0337710:CDS 45.0%
ACACTTGCAAGCTGTGTCAA+CGG + chr2_4:57793480-57793499 Msa0337710:CDS 45.0%
GAATGTGGACAATGCTTTCG+TGG + chr2_4:57793570-57793589 Msa0337710:CDS 45.0%
! GCTCAGATTTGGGATACTGC+TGG + chr2_4:57792226-57792245 Msa0337710:CDS 50.0%
GGGATACTGCTGGTCAAGAA+AGG + chr2_4:57792236-57792255 Msa0337710:CDS 50.0%
TGCTGGTGTACGACTCAACA+AGG + chr2_4:57793356-57793375 Msa0337710:CDS 50.0%
TGCACTAGAGTCGCTGAATG+TGG + chr2_4:57793555-57793574 Msa0337710:CDS 50.0%
! GCTCAGATTTGGGATACTGC+TGG + chr2_4:57792226-57792245 Msa0337710:CDS 50.0%
GGGATACTGCTGGTCAAGAA+AGG + chr2_4:57792236-57792255 Msa0337710:CDS 50.0%
TGCTGGTGTACGACTCAACA+AGG + chr2_4:57793356-57793375 Msa0337710:CDS 50.0%
TGCACTAGAGTCGCTGAATG+TGG + chr2_4:57793555-57793574 Msa0337710:CDS 50.0%
GCATACTACCGTGGAGCTGT+TGG + chr2_4:57793328-57793347 Msa0337710:CDS 55.0%
GCATACTACCGTGGAGCTGT+TGG + chr2_4:57793328-57793347 Msa0337710:CDS 55.0%
GCAATGCGCCAACAGCTCCA+CGG - chr2_4:57793339-57793358 None:intergenic 60.0%
TGGAGCTGTTGGCGCATTGC+TGG + chr2_4:57793339-57793358 Msa0337710:CDS 60.0%
! TGGCTCTGCGTCAGTGCCTA+AGG + chr2_4:57793648-57793667 Msa0337710:CDS 60.0%
GGCTCTGCGTCAGTGCCTAA+GGG + chr2_4:57793649-57793668 Msa0337710:CDS 60.0%
GCAATGCGCCAACAGCTCCA+CGG - chr2_4:57793339-57793358 None:intergenic 60.0%
TGGAGCTGTTGGCGCATTGC+TGG + chr2_4:57793339-57793358 Msa0337710:CDS 60.0%
! TGGCTCTGCGTCAGTGCCTA+AGG + chr2_4:57793648-57793667 Msa0337710:CDS 60.0%
GGCTCTGCGTCAGTGCCTAA+GGG + chr2_4:57793649-57793668 Msa0337710:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 57791899 57793977 57791899 ID=Msa0337710;Name=Msa0337710
chr2_4 mRNA 57791899 57793977 57791899 ID=Msa0337710-mRNA-1;Parent=Msa0337710;Name=Msa0337710-mRNA-1;_AED=0.05;_eAED=0.05;_QI=144|1|1|1|1|1|2|236|215
chr2_4 exon 57791899 57792257 57791899 ID=Msa0337710-mRNA-1:exon:17630;Parent=Msa0337710-mRNA-1
chr2_4 exon 57793309 57793977 57793309 ID=Msa0337710-mRNA-1:exon:17631;Parent=Msa0337710-mRNA-1
chr2_4 five_prime_UTR 57791899 57792042 57791899 ID=Msa0337710-mRNA-1:five_prime_utr;Parent=Msa0337710-mRNA-1
chr2_4 CDS 57792043 57792257 57792043 ID=Msa0337710-mRNA-1:cds;Parent=Msa0337710-mRNA-1
chr2_4 CDS 57793309 57793741 57793309 ID=Msa0337710-mRNA-1:cds;Parent=Msa0337710-mRNA-1
chr2_4 three_prime_UTR 57793742 57793977 57793742 ID=Msa0337710-mRNA-1:three_prime_utr;Parent=Msa0337710-mRNA-1
Gene Sequence

>Msa0337710

ATGGGTGCTGTAGACGAAGAATACGATTACTTGTTCAAGTTGGTTTTGATCGGTGATTCTGGTGTTGGAAAGTCCAATCTTTTATCTAGATTCACTAGAAATGAATTCAACTTGGAATCCAAATCCACCATCGGTGTTGAATTTGCTACTCGCAGTGTCAGGATTCTTGATAAGCTTGTTAAGGCTCAGATTTGGGATACTGCTGGTCAAGAAAGATATCGTGCAATCACAAGTGCATACTACCGTGGAGCTGTTGGCGCATTGCTGGTGTACGACTCAACAAGGCATGTGACATTTGAAAATATTGAGAGGTGGTTGAAGGAGCTTCGAGATCATACAGATGCCTATGTTGTAATCATGCTTGTAGGAAACAAAGCAGATCTTCGACACTTGCAAGCTGTGTCAACGGAAGAAGCCACCACATTTGCTGAGAAAGAAAACATATATTTCATGGAAACTTCTGCACTAGAGTCGCTGAATGTGGACAATGCTTTCGTGGAAGTGCTCACTCAAATATACAATGTTGTGAGTAAGAAGACTCTTGAGAAAGAGAATGGCTCTGCGTCAGTGCCTAAGGGAGAGACCATCAATATTGGTAAAGATGATGTTTCAGATGTTAAGAAGAGTGGATGCTGCAGTACAGCATAG
Protein sequence

>Msa0337710

MGAVDEEYDYLFKLVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSVRILDKLVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDSTRHVTFENIERWLKELRDHTDAYVVIMLVGNKADLRHLQAVSTEEATTFAEKENIYFMETSALESLNVDNAFVEVLTQIYNVVSKKTLEKENGSASVPKGETINIGKDDVSDVKKSGCCSTA*