AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0135990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135990 MtrunA17_Chr1g0207011 92.347 196 10 4 1 191 3 198 1.70e-118 333
Msa0135990 MtrunA17_Chr5g0393821 50.510 196 72 11 1 187 1 180 5.48e-42 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135990 AT4G33800.2 41.040 173 77 9 9 169 5 164 2.03e-21 86.7
Msa0135990 AT4G33800.1 41.040 173 77 9 9 169 5 164 2.03e-21 86.7

Find 57 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACATGTTCATTATCCTTT+AGG 0.222495 1_3:+67772825 Msa0135980:five_prime_UTR
ATGGTTTCTGATGCTTCTTC+TGG 0.266605 1_3:-67777599 Msa0135990:CDS
AGCACCACCAACATGGAATT+TGG 0.289275 1_3:+67772998 Msa0135980:CDS
TCATACCAGGAAGAACAATT+TGG 0.315479 1_3:-67777084 Msa0135990:CDS
ACAAAGTTATGTAGGAAATA+TGG 0.330154 1_3:-67777246 Msa0135990:CDS
TATGAAAACACTAGATTCAT+AGG 0.343147 1_3:-67777704 Msa0135990:CDS
TAGCTAGAAAGAATTGAAAA+AGG 0.343625 1_3:-67777799 Msa0135990:five_prime_UTR
GAAGAGGCGATGAGTTCTAT+AGG 0.348824 1_3:+67772875 Msa0135980:exon
TATAGGGCAAACCATGTTAA+TGG 0.362210 1_3:+67772892 Msa0135980:CDS
TATTTCCTACATAACTTTGT+TGG 0.375448 1_3:+67777249 None:intergenic
TGTAGTAGTGGATGTGAGTC+AGG 0.376527 1_3:-67777752 Msa0135990:CDS
CTTCTGAATGTATGCGTGTC+TGG 0.391835 1_3:+67773156 Msa0135980:intron
AGGAGTGGAAGATTTGTCTA+TGG 0.397750 1_3:-67777618 Msa0135990:CDS
AAATTGTTCTTCCTGGTATG+AGG 0.406008 1_3:+67777086 None:intergenic
GCTTAATAAACTTATCCTAA+AGG 0.408244 1_3:-67772840 Msa0135990:three_prime_UTR
TCTTTGTTTAGCAGCTGGTA+TGG 0.422441 1_3:-67776929 Msa0135990:intron
GGTGGAACTGAAGGGTACTA+TGG 0.434994 1_3:-67777683 Msa0135990:CDS
CCTTCCAAATTCCATGTTGG+TGG 0.438290 1_3:-67773002 Msa0135990:three_prime_UTR
TTGGTGGTGCTTCTCAAAGT+TGG 0.441638 1_3:-67772986 Msa0135990:three_prime_UTR
TGGTGGTGCTTCTCAAAGTT+GGG 0.449042 1_3:-67772985 Msa0135990:three_prime_UTR
AGGGTCTTGCGTTGCGGTTG+CGG 0.451542 1_3:-67773053 Msa0135990:three_prime_UTR
AAGAGGCGATGAGTTCTATA+GGG 0.479187 1_3:+67772876 Msa0135980:exon
AGAAGCAATGTCCTTGACTC+AGG 0.482163 1_3:-67773073 Msa0135990:three_prime_UTR
CAGAAGTGATGACGCTACAT+AGG 0.486769 1_3:-67773139 Msa0135990:three_prime_UTR
GCAGTGTCATGAAGAAAAGA+AGG 0.507146 1_3:+67777443 None:intergenic
GGTACAAAGAAACAGAAAGT+TGG 0.512053 1_3:-67777506 Msa0135990:CDS
ACAAACCAACAAAGTTATGT+AGG 0.515352 1_3:-67777254 Msa0135990:CDS
TTGGTTTGTCCCAACAACAT+TGG 0.519643 1_3:+67777268 None:intergenic
TGCTATTGAAGTTGTTGCAG+TGG 0.524195 1_3:-67776874 Msa0135990:three_prime_UTR
TCTCCTTCCAAATTCCATGT+TGG 0.526459 1_3:-67773005 Msa0135990:three_prime_UTR
GTTAATGGCACTAACAGTTA+CGG 0.549686 1_3:+67772907 Msa0135980:CDS
AACTGTTAGTGCCATTAACA+TGG 0.549756 1_3:-67772903 Msa0135990:three_prime_UTR
GTATGCATCATCCAATGTGC+AGG 0.549946 1_3:+67772937 Msa0135980:CDS
AGAAGTGATGACGCTACATA+GGG 0.557585 1_3:-67773138 Msa0135990:three_prime_UTR
TGACTCAGGGTCTTGCGTTG+CGG 0.557938 1_3:-67773059 Msa0135990:three_prime_UTR
ACATCATCATGTGGAAGATG+TGG 0.558107 1_3:+67777572 None:intergenic
AGATTCATAGGTGGAACTGA+AGG 0.568350 1_3:-67777692 Msa0135990:CDS
GTCACAAATGAGTATTCTGA+AGG 0.574749 1_3:-67777638 Msa0135990:CDS
AACATGGAATTTGGAAGGAG+AGG 0.586422 1_3:+67773007 Msa0135980:CDS
ATTATGAACTGCCTGCACAT+TGG 0.588435 1_3:-67772948 Msa0135990:three_prime_UTR
AAATGAGTATTCTGAAGGAG+TGG 0.595088 1_3:-67777633 Msa0135990:CDS
TTGTTTAGCAGCTGGTATGG+AGG 0.596105 1_3:-67776926 Msa0135990:intron
TTTGATTCTGAATGTAGTAG+TGG 0.600879 1_3:-67777764 Msa0135990:CDS
GAAGCAATGTCCTTGACTCA+GGG 0.617035 1_3:-67773072 Msa0135990:three_prime_UTR
CCACCAACATGGAATTTGGA+AGG 0.619705 1_3:+67773002 Msa0135980:CDS
GTAGGGAAAATCCTCATACC+AGG 0.622430 1_3:-67777097 Msa0135990:intron
TGCGGTTGCGGCGATAACAG+TGG 0.623047 1_3:-67773041 Msa0135990:three_prime_UTR
CAACGCAAGACCCTGAGTCA+AGG 0.623179 1_3:+67773062 Msa0135980:CDS
TTTGAGAAGCACCACCAACA+TGG 0.627251 1_3:+67772991 Msa0135980:CDS
TGCAACAACTTCAATAGCAG+AGG 0.636583 1_3:+67776878 None:intergenic
TCATCATGTGGAAGATGTGG+AGG 0.636849 1_3:+67777575 None:intergenic
TTGAAGTAAACATCATCATG+TGG 0.637487 1_3:+67777563 None:intergenic
GATTCATAGGTGGAACTGAA+GGG 0.638366 1_3:-67777691 Msa0135990:CDS
GTAGTGGATGTGAGTCAGGT+TGG 0.642732 1_3:-67777748 Msa0135990:CDS
TGTCATGAAGAAAAGAAGGA+AGG 0.667075 1_3:+67777447 None:intergenic
GAAAACACTAGATTCATAGG+TGG 0.672436 1_3:-67777701 Msa0135990:CDS
AATATGGTAGACTATTCACA+AGG 0.724521 1_3:-67777230 Msa0135990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAGATATTGAAATTATA+TGG - chr1_3:67774199-67774218 Msa0135990:intron 10.0%
!! ATTATTTCAATGTAAATAAA+AGG + chr1_3:67774402-67774421 None:intergenic 10.0%
!! AAAAGAATTAATACAATTAA+GGG + chr1_3:67774446-67774465 None:intergenic 10.0%
!! AAAAAGAATTAATACAATTA+AGG + chr1_3:67774447-67774466 None:intergenic 10.0%
!!! TATAAACAAAATCATTTTTT+AGG - chr1_3:67774651-67774670 Msa0135990:intron 10.0%
!! AAATTATAAATCATATACAT+TGG - chr1_3:67774701-67774720 Msa0135990:intron 10.0%
!! AATTACTTATTTAATTAAAC+AGG + chr1_3:67775250-67775269 None:intergenic 10.0%
!!! ATATCATATGTTTTAATTAA+TGG + chr1_3:67776477-67776496 None:intergenic 10.0%
!!! TTTTAAAAAGATGATATATA+TGG + chr1_3:67776736-67776755 None:intergenic 10.0%
!! CATGTGATTATATAAAAAAT+CGG - chr1_3:67774582-67774601 Msa0135990:intron 15.0%
!! TCACTAATAACTTATTTATT+TGG - chr1_3:67774675-67774694 Msa0135990:intron 15.0%
!! AAAGGAATAAAAAAAATCAA+AGG + chr1_3:67775209-67775228 None:intergenic 15.0%
!!! TATACTATAATCTAAAGTTT+TGG - chr1_3:67775373-67775392 Msa0135990:intron 15.0%
!!! AATATTCCTATACAATTTTA+TGG + chr1_3:67775538-67775557 None:intergenic 15.0%
!! AAATTGTATAGGAATATTAA+AGG - chr1_3:67775540-67775559 Msa0135990:intron 15.0%
!! AATTGTATAGGAATATTAAA+GGG - chr1_3:67775541-67775560 Msa0135990:intron 15.0%
!!! TCTTTTTAAAAGTTTTTCAT+AGG - chr1_3:67776745-67776764 Msa0135990:intron 15.0%
!!! ACTAATTATTTTGAGGTAAT+TGG - chr1_3:67773471-67773490 Msa0135990:intron 20.0%
!!! AATTTGTTTGTGTTATTTGT+AGG - chr1_3:67773553-67773572 Msa0135990:intron 20.0%
!!! ATTTGTTTGTGTTATTTGTA+GGG - chr1_3:67773554-67773573 Msa0135990:intron 20.0%
!! TAATTGATAAATTAATCCGA+AGG + chr1_3:67774240-67774259 None:intergenic 20.0%
!!! GTGTATGTACTTTTATTTTT+TGG + chr1_3:67774311-67774330 None:intergenic 20.0%
!! CCATATATTATAACATTTGT+CGG + chr1_3:67774500-67774519 None:intergenic 20.0%
!! CAAAAAAAACTAAACGAAAT+GGG + chr1_3:67775051-67775070 None:intergenic 20.0%
!! ACAAAAAAAACTAAACGAAA+TGG + chr1_3:67775052-67775071 None:intergenic 20.0%
!!! AGCTTCTAAAAAAATTATTG+TGG - chr1_3:67776185-67776204 Msa0135990:intron 20.0%
!!! GCTTCTAAAAAAATTATTGT+GGG - chr1_3:67776186-67776205 Msa0135990:intron 20.0%
!! AAACTAATAACATGTACAAT+TGG - chr1_3:67776345-67776364 Msa0135990:intron 20.0%
!!! TGTTTTAATTAATGGAGAAT+TGG + chr1_3:67776469-67776488 None:intergenic 20.0%
!!! GTAAATTTTGATTTTCTGTT+GGG + chr1_3:67777006-67777025 None:intergenic 20.0%
!!! TGTAAATTTTGATTTTCTGT+TGG + chr1_3:67777007-67777026 None:intergenic 20.0%
!! AAAATCAAAATTTACAAGCT+AGG - chr1_3:67777011-67777030 Msa0135990:intron 20.0%
! TAGCTAGAAAGAATTGAAAA+AGG - chr1_3:67772869-67772888 Msa0135990:three_prime_UTR 25.0%
!! TGTTTTCATAAGAATGTTCA+AGG + chr1_3:67772954-67772973 Msa0135980:CDS 25.0%
! TATGAAAACACTAGATTCAT+AGG - chr1_3:67772964-67772983 Msa0135990:three_prime_UTR 25.0%
!! CTTTTCAACTGTAAGAAATA+TGG - chr1_3:67773266-67773285 Msa0135990:intron 25.0%
! TATTTCCTACATAACTTTGT+TGG + chr1_3:67773422-67773441 Msa0135980:intron 25.0%
! ACAAAGTTATGTAGGAAATA+TGG - chr1_3:67773422-67773441 Msa0135990:intron 25.0%
!!! TTCAGCAACTAATTATTTTG+AGG - chr1_3:67773464-67773483 Msa0135990:intron 25.0%
! TGTTACTCAAAGATTAATGA+AGG - chr1_3:67773818-67773837 Msa0135990:intron 25.0%
! ACTGAATTGACAAAGTAAAA+AGG + chr1_3:67773861-67773880 None:intergenic 25.0%
!! TTTTTACTTTGTCAATTCAG+TGG - chr1_3:67773860-67773879 Msa0135990:intron 25.0%
!!! GTAATTTTGTTGTTAATCGA+TGG - chr1_3:67773931-67773950 Msa0135990:intron 25.0%
! GATTACAAGCTAATTTAGTT+AGG - chr1_3:67773955-67773974 Msa0135990:intron 25.0%
! GTAGATTCATCAAAATAACA+AGG + chr1_3:67774006-67774025 None:intergenic 25.0%
! TTTCAGTACATTAAGATGTA+CGG + chr1_3:67774356-67774375 None:intergenic 25.0%
! GAATTAATACAATTAAGGGT+GGG + chr1_3:67774442-67774461 None:intergenic 25.0%
! AGAATTAATACAATTAAGGG+TGG + chr1_3:67774443-67774462 None:intergenic 25.0%
! CCGACAAATGTTATAATATA+TGG - chr1_3:67774497-67774516 Msa0135990:intron 25.0%
! TAGCTTATAAACGAATCTAA+AGG + chr1_3:67774757-67774776 None:intergenic 25.0%
! AATCAGATTACATGCATATT+TGG + chr1_3:67774841-67774860 None:intergenic 25.0%
!! TGATTCTAGAAAAGAGAATA+TGG - chr1_3:67774856-67774875 Msa0135990:intron 25.0%
!! TTTGTCAACTTTATCTCTTT+TGG - chr1_3:67775067-67775086 Msa0135990:intron 25.0%
!! TTGTCAACTTTATCTCTTTT+GGG - chr1_3:67775068-67775087 Msa0135990:intron 25.0%
! AATTGACTAGGAATAAGTAA+AGG + chr1_3:67775227-67775246 None:intergenic 25.0%
! TAATTAAACAGGAATTGACT+AGG + chr1_3:67775239-67775258 None:intergenic 25.0%
!!! CTTAACTTTTTCAACCTTTA+TGG + chr1_3:67775289-67775308 None:intergenic 25.0%
! CATAAAGGTTGAAAAAGTTA+AGG - chr1_3:67775287-67775306 Msa0135990:intron 25.0%
!! AATATGACCTTTGAGAATTT+AGG - chr1_3:67775312-67775331 Msa0135990:intron 25.0%
! AAAAACCTAAGTAAAGAAGA+AGG - chr1_3:67775423-67775442 Msa0135990:intron 25.0%
! TGCAATCCATAAAATTGTAT+AGG - chr1_3:67775529-67775548 Msa0135990:intron 25.0%
! TATAGGAATATTAAAGGGTA+TGG - chr1_3:67775546-67775565 Msa0135990:intron 25.0%
!! ATAGGAATATTAAAGGGTAT+GGG - chr1_3:67775547-67775566 Msa0135990:intron 25.0%
! ATAAAGAGGATATCAGAAAA+TGG + chr1_3:67775677-67775696 None:intergenic 25.0%
! AACATTTAACCTCAAATCAA+CGG + chr1_3:67775908-67775927 None:intergenic 25.0%
! TATCCAATTTCCAATTTCAT+TGG + chr1_3:67776390-67776409 None:intergenic 25.0%
!!! GTTTTGAAATAGAGTTCAAA+AGG - chr1_3:67776508-67776527 Msa0135990:intron 25.0%
!!! TTAATGAAAAGTGTTAGACT+TGG + chr1_3:67776856-67776875 None:intergenic 25.0%
! AGATTACCAAATAGAAAAGA+GGG + chr1_3:67776928-67776947 None:intergenic 25.0%
! TAGATTACCAAATAGAAAAG+AGG + chr1_3:67776929-67776948 None:intergenic 25.0%
! TTATTACAAGCGTAATTACA+AGG + chr1_3:67777184-67777203 None:intergenic 25.0%
! GCTTAATAAACTTATCCTAA+AGG - chr1_3:67777828-67777847 Msa0135990:five_prime_UTR 25.0%
TTTGATTCTGAATGTAGTAG+TGG - chr1_3:67772904-67772923 Msa0135990:three_prime_UTR 30.0%
TTGAAGTAAACATCATCATG+TGG + chr1_3:67773108-67773127 Msa0135980:intron 30.0%
!! ATTTTTCAGACCAATGTTGT+TGG - chr1_3:67773390-67773409 Msa0135990:intron 30.0%
!! TTTTTCAGACCAATGTTGTT+GGG - chr1_3:67773391-67773410 Msa0135990:intron 30.0%
ACAAACCAACAAAGTTATGT+AGG - chr1_3:67773414-67773433 Msa0135990:intron 30.0%
AATATGGTAGACTATTCACA+AGG - chr1_3:67773438-67773457 Msa0135990:intron 30.0%
AAAAACCAAATTGTTCTTCC+TGG + chr1_3:67773592-67773611 Msa0135980:exon 30.0%
!!! AGTTCATTTTTTGAGAGAGA+TGG + chr1_3:67773620-67773639 Msa0135980:intron 30.0%
CTCTCAAAAAATGAACTTCA+AGG - chr1_3:67773623-67773642 Msa0135990:intron 30.0%
ACAAGCTAATTTAGTTAGGA+AGG - chr1_3:67773959-67773978 Msa0135990:intron 30.0%
! AGTAGAACAGATTTTGAATG+AGG + chr1_3:67774141-67774160 None:intergenic 30.0%
GTTGTATATTACTCAACCTT+CGG - chr1_3:67774221-67774240 Msa0135990:intron 30.0%
! AATTTAGTCACACACTGATT+AGG - chr1_3:67774548-67774567 Msa0135990:intron 30.0%
GGAATTTGACAAAATTACGA+GGG + chr1_3:67774810-67774829 None:intergenic 30.0%
AGGAATTTGACAAAATTACG+AGG + chr1_3:67774811-67774830 None:intergenic 30.0%
! CGTAATTTTGTCAAATTCCT+AGG - chr1_3:67774811-67774830 Msa0135990:intron 30.0%
!! ATATGGCAAAAGTGTTATGA+AGG - chr1_3:67774873-67774892 Msa0135990:intron 30.0%
!! TATGGCAAAAGTGTTATGAA+GGG - chr1_3:67774874-67774893 Msa0135990:intron 30.0%
GACAGTATACAAATATGATG+AGG - chr1_3:67774909-67774928 Msa0135990:intron 30.0%
AGTATACAAATATGATGAGG+CGG - chr1_3:67774912-67774931 Msa0135990:intron 30.0%
TTCCATTCCAACATCAATAT+TGG + chr1_3:67774943-67774962 None:intergenic 30.0%
TTGTCATCCTAAATTCTCAA+AGG + chr1_3:67775322-67775341 None:intergenic 30.0%
TAGTATATGAGAAAACACGT+GGG + chr1_3:67775360-67775379 None:intergenic 30.0%
ATAGTATATGAGAAAACACG+TGG + chr1_3:67775361-67775380 None:intergenic 30.0%
GTTAACCTTCTTCTTTACTT+AGG + chr1_3:67775431-67775450 None:intergenic 30.0%
! TACGTGTTTGATTTCTCTTT+GGG + chr1_3:67775453-67775472 None:intergenic 30.0%
! TTACGTGTTTGATTTCTCTT+TGG + chr1_3:67775454-67775473 None:intergenic 30.0%
ATTACAACAAGGATAGATAG+AGG + chr1_3:67775623-67775642 None:intergenic 30.0%
CCTCTATTACAATTACAACA+AGG + chr1_3:67775634-67775653 None:intergenic 30.0%
! CCTTGTTGTAATTGTAATAG+AGG - chr1_3:67775631-67775650 Msa0135990:intron 30.0%
TGTTGTAATTGTAATAGAGG+AGG - chr1_3:67775634-67775653 Msa0135990:intron 30.0%
! TCAGAAAATGGACAAGTTTT+TGG + chr1_3:67775665-67775684 None:intergenic 30.0%
AAAGTTATGTCAAGGGAATT+GGG + chr1_3:67775729-67775748 None:intergenic 30.0%
AAAAGTTATGTCAAGGGAAT+TGG + chr1_3:67775730-67775749 None:intergenic 30.0%
TCTCATGCTGTAAATGATTT+CGG - chr1_3:67775882-67775901 Msa0135990:intron 30.0%
GAGGTTAAATGTTTCGATTT+CGG - chr1_3:67775915-67775934 Msa0135990:intron 30.0%
!! AGCTCAACAATAGTTCTAAA+CGG - chr1_3:67776126-67776145 Msa0135990:intron 30.0%
!! GGTGGATTAGAATCTATAAT+TGG - chr1_3:67776162-67776181 Msa0135990:intron 30.0%
! CTTAAAAGCACAAAACAAGA+TGG - chr1_3:67776542-67776561 Msa0135990:intron 30.0%
TAATGGTAGATCTCATTTGA+TGG - chr1_3:67776618-67776637 Msa0135990:intron 30.0%
CTATTACTTTCATGCATTCT+TGG + chr1_3:67776708-67776727 None:intergenic 30.0%
! AAGTTTTTCATAGGTTTCGT+CGG - chr1_3:67776754-67776773 Msa0135990:intron 30.0%
ATGGTCAACTAAAGAAGATT+AGG + chr1_3:67776880-67776899 None:intergenic 30.0%
TTGACCATACATATCTTACT+AGG - chr1_3:67776892-67776911 Msa0135990:exon 30.0%
TTTGCCTAGTAAGATATGTA+TGG + chr1_3:67776899-67776918 None:intergenic 30.0%
! TGATTTTCTGTTGGGAAATT+CGG + chr1_3:67776998-67777017 None:intergenic 30.0%
ATCAAAATTTACAAGCTAGG+TGG - chr1_3:67777014-67777033 Msa0135990:intron 30.0%
! ATTACTTTGAGTTCGTAGAA+GGG + chr1_3:67777161-67777180 None:intergenic 30.0%
!! TTTAATCACAGTTTTGTGTG+TGG + chr1_3:67777221-67777240 None:intergenic 30.0%
AAGCAAACTAAAAAGAGAAG+AGG + chr1_3:67777812-67777831 None:intergenic 30.0%
GAAAACACTAGATTCATAGG+TGG - chr1_3:67772967-67772986 Msa0135990:three_prime_UTR 35.0%
GTCACAAATGAGTATTCTGA+AGG - chr1_3:67773030-67773049 Msa0135990:three_prime_UTR 35.0%
! AAATGAGTATTCTGAAGGAG+TGG - chr1_3:67773035-67773054 Msa0135990:three_prime_UTR 35.0%
! GTAGTGAAAAAGCACAAGAA+AGG - chr1_3:67773141-67773160 Msa0135990:three_prime_UTR 35.0%
GGTACAAAGAAACAGAAAGT+TGG - chr1_3:67773162-67773181 Msa0135990:three_prime_UTR 35.0%
TGTCATGAAGAAAAGAAGGA+AGG + chr1_3:67773224-67773243 Msa0135980:intron 35.0%
AAATTGTTCTTCCTGGTATG+AGG + chr1_3:67773585-67773604 Msa0135980:CDS 35.0%
TCATACCAGGAAGAACAATT+TGG - chr1_3:67773584-67773603 Msa0135990:intron 35.0%
! TTCATTCTTTGTTTAGCAGC+TGG - chr1_3:67773734-67773753 Msa0135990:intron 35.0%
CACAATCTAAATCCATGGAA+TGG - chr1_3:67774274-67774293 Msa0135990:intron 35.0%
GAACATATACTTCCATTCCA+TGG + chr1_3:67774289-67774308 None:intergenic 35.0%
AAGGGTGGGAAAAAACTTTA+CGG + chr1_3:67774428-67774447 None:intergenic 35.0%
!! CAAAAGTGTTATGAAGGGAT+GGG - chr1_3:67774879-67774898 Msa0135990:intron 35.0%
GGAAATGCCAATATTGATGT+TGG - chr1_3:67774933-67774952 Msa0135990:intron 35.0%
TGCCAATATTGATGTTGGAA+TGG - chr1_3:67774938-67774957 Msa0135990:intron 35.0%
! TCAACTTTATCTCTTTTGGG+AGG - chr1_3:67775071-67775090 Msa0135990:intron 35.0%
!!! CTTTTTCAACCTTTATGGAC+TGG + chr1_3:67775284-67775303 None:intergenic 35.0%
AGTATATGAGAAAACACGTG+GGG + chr1_3:67775359-67775378 None:intergenic 35.0%
!! CAATTTTATGGATTGCAGCT+AGG + chr1_3:67775526-67775545 None:intergenic 35.0%
TGACCATTGCCAATTTAGTT+TGG - chr1_3:67775698-67775717 Msa0135990:intron 35.0%
CATCCAAACTAAATTGGCAA+TGG + chr1_3:67775704-67775723 None:intergenic 35.0%
TGGGAACATCCAAACTAAAT+TGG + chr1_3:67775710-67775729 None:intergenic 35.0%
GTGCACAAAAGTTATGTCAA+GGG + chr1_3:67775736-67775755 None:intergenic 35.0%
AGTGCACAAAAGTTATGTCA+AGG + chr1_3:67775737-67775756 None:intergenic 35.0%
GTGTAGCACATTTATTTCCT+TGG + chr1_3:67775781-67775800 None:intergenic 35.0%
! TTTGGTGTGACATAATGAGT+TGG + chr1_3:67775829-67775848 None:intergenic 35.0%
CATTATGTCACACCAAAAAC+TGG - chr1_3:67775832-67775851 Msa0135990:intron 35.0%
TGCAGTTTGACCTAAATCAA+TGG + chr1_3:67775941-67775960 None:intergenic 35.0%
TATTGTTACACCATGCAGTA+GGG + chr1_3:67776032-67776051 None:intergenic 35.0%
TTATTGTTACACCATGCAGT+AGG + chr1_3:67776033-67776052 None:intergenic 35.0%
CTAAACGGACACTAATATCA+CGG - chr1_3:67776141-67776160 Msa0135990:intron 35.0%
TGTGGGTAAACACTAACTTT+AGG - chr1_3:67776203-67776222 Msa0135990:intron 35.0%
TAAACACTAACTTTAGGTGG+TGG - chr1_3:67776209-67776228 Msa0135990:intron 35.0%
CTTCACGTTTAGTCAATAGT+CGG - chr1_3:67776241-67776260 Msa0135990:intron 35.0%
GTGCCAATGAAATTGGAAAT+TGG - chr1_3:67776384-67776403 Msa0135990:intron 35.0%
CTCATGACTTTGGATCAATT+TGG + chr1_3:67776574-67776593 None:intergenic 35.0%
! GATCTACCATTATGTCACTT+AGG + chr1_3:67776610-67776629 None:intergenic 35.0%
!! ATGAAAAGTGTTAGACTTGG+AGG + chr1_3:67776853-67776872 None:intergenic 35.0%
! AACCATCCCTCTTTTCTATT+TGG - chr1_3:67776919-67776938 Msa0135990:intron 35.0%
TACCAAATAGAAAAGAGGGA+TGG + chr1_3:67776924-67776943 None:intergenic 35.0%
!! TGTTAGCTTTAAGCCAATCA+AGG - chr1_3:67777085-67777104 Msa0135990:CDS 35.0%
AAGCTTTCAGAAGCAGAAAA+GGG + chr1_3:67777114-67777133 None:intergenic 35.0%
! TTACTTTGAGTTCGTAGAAG+GGG + chr1_3:67777160-67777179 None:intergenic 35.0%
! GATTACTTTGAGTTCGTAGA+AGG + chr1_3:67777162-67777181 None:intergenic 35.0%
AAATGACAGAGAGGTAAGTA+GGG + chr1_3:67777286-67777305 None:intergenic 35.0%
AAAATGACAGAGAGGTAAGT+AGG + chr1_3:67777287-67777306 None:intergenic 35.0%
!!! CGACATGAACATGTTTTTAG+TGG + chr1_3:67777390-67777409 None:intergenic 35.0%
TAATCAAATGTGACTGTGTC+AGG - chr1_3:67777476-67777495 Msa0135990:CDS 35.0%
GTTAATGGCACTAACAGTTA+CGG + chr1_3:67777764-67777783 None:intergenic 35.0%
! AACTGTTAGTGCCATTAACA+TGG - chr1_3:67777765-67777784 Msa0135990:CDS 35.0%
TATAGGGCAAACCATGTTAA+TGG + chr1_3:67777779-67777798 None:intergenic 35.0%
AGATTCATAGGTGGAACTGA+AGG - chr1_3:67772976-67772995 Msa0135990:three_prime_UTR 40.0%
GATTCATAGGTGGAACTGAA+GGG - chr1_3:67772977-67772996 Msa0135990:three_prime_UTR 40.0%
! TGACTCTTTTGTCTCTGTGT+TGG + chr1_3:67773014-67773033 Msa0135980:CDS 40.0%
AGGAGTGGAAGATTTGTCTA+TGG - chr1_3:67773050-67773069 Msa0135990:three_prime_UTR 40.0%
ATGGTTTCTGATGCTTCTTC+TGG - chr1_3:67773069-67773088 Msa0135990:three_prime_UTR 40.0%
ACATCATCATGTGGAAGATG+TGG + chr1_3:67773099-67773118 Msa0135980:intron 40.0%
GCAGTGTCATGAAGAAAAGA+AGG + chr1_3:67773228-67773247 Msa0135980:intron 40.0%
!! TTGGTTTGTCCCAACAACAT+TGG + chr1_3:67773403-67773422 Msa0135980:intron 40.0%
! TCTTTGTTTAGCAGCTGGTA+TGG - chr1_3:67773739-67773758 Msa0135990:intron 40.0%
TGCAACAACTTCAATAGCAG+AGG + chr1_3:67773793-67773812 Msa0135980:three_prime_UTR 40.0%
TGCTATTGAAGTTGTTGCAG+TGG - chr1_3:67773794-67773813 Msa0135990:intron 40.0%
GCATGCACAATCTAAATCCA+TGG - chr1_3:67774269-67774288 Msa0135990:intron 40.0%
! TAGTCACACACTGATTAGGT+AGG - chr1_3:67774552-67774571 Msa0135990:intron 40.0%
CATGCATATTTGGATTGCCT+AGG + chr1_3:67774831-67774850 None:intergenic 40.0%
!! GCAAAAGTGTTATGAAGGGA+TGG - chr1_3:67774878-67774897 Msa0135990:intron 40.0%
CTTTGAGTCAGGAAGATATG+AGG + chr1_3:67775011-67775030 None:intergenic 40.0%
CTCATATCTTCCTGACTCAA+AGG - chr1_3:67775009-67775028 Msa0135990:intron 40.0%
ATTCTGCTACCAGTCCATAA+AGG - chr1_3:67775272-67775291 Msa0135990:intron 40.0%
!! TTGGTTTGGTTTGGTTTGGA+TGG + chr1_3:67775599-67775618 None:intergenic 40.0%
AACAAGGATAGATAGAGGCT+TGG + chr1_3:67775618-67775637 None:intergenic 40.0%
TTGGCAATGGTCACATAAAG+AGG + chr1_3:67775691-67775710 None:intergenic 40.0%
! GCATATCTCGATCCAGTTTT+TGG + chr1_3:67775847-67775866 None:intergenic 40.0%
!! TCGATTTCGGCCATTGATTT+AGG - chr1_3:67775928-67775947 Msa0135990:intron 40.0%
! AAAAGTTGATCCCTACTGCA+TGG - chr1_3:67776019-67776038 Msa0135990:intron 40.0%
AGAACTATTGTTGAGCTGCA+CGG + chr1_3:67776123-67776142 None:intergenic 40.0%
GGGTAAACACTAACTTTAGG+TGG - chr1_3:67776206-67776225 Msa0135990:intron 40.0%
TACACGAGTGCCAATGAAAT+TGG - chr1_3:67776377-67776396 Msa0135990:intron 40.0%
ATTGATCCAAAGTCATGAGC+AGG - chr1_3:67776575-67776594 Msa0135990:intron 40.0%
CGCAAACCTAAGTGACATAA+TGG - chr1_3:67776601-67776620 Msa0135990:intron 40.0%
CATTGCCTCAAATATGACTC+TGG + chr1_3:67776780-67776799 None:intergenic 40.0%
GGTACAGTTTCTTTCTTGCA+AGG + chr1_3:67776971-67776990 None:intergenic 40.0%
TCTGTTGGGAAATTCGGATA+TGG + chr1_3:67776992-67777011 None:intergenic 40.0%
!! CAGAAAAGGGTATCCTTGAT+TGG + chr1_3:67777101-67777120 None:intergenic 40.0%
GAAGCTTTCAGAAGCAGAAA+AGG + chr1_3:67777115-67777134 None:intergenic 40.0%
! TACTTTGAGTTCGTAGAAGG+GGG + chr1_3:67777159-67777178 None:intergenic 40.0%
AATGACAGAGAGGTAAGTAG+GGG + chr1_3:67777285-67777304 None:intergenic 40.0%
CAAGGAGAAAAATGACAGAG+AGG + chr1_3:67777295-67777314 None:intergenic 40.0%
! AGAAGTGATGACGCTACATA+GGG - chr1_3:67777530-67777549 Msa0135990:CDS 40.0%
AACATGGAATTTGGAAGGAG+AGG + chr1_3:67777664-67777683 None:intergenic 40.0%
TCTCCTTCCAAATTCCATGT+TGG - chr1_3:67777663-67777682 Msa0135990:CDS 40.0%
ATTATGAACTGCCTGCACAT+TGG - chr1_3:67777720-67777739 Msa0135990:CDS 40.0%
AAGAGGCGATGAGTTCTATA+GGG + chr1_3:67777795-67777814 None:intergenic 40.0%
!! TGTAGTAGTGGATGTGAGTC+AGG - chr1_3:67772916-67772935 Msa0135990:three_prime_UTR 45.0%
TCATCATGTGGAAGATGTGG+AGG + chr1_3:67773096-67773115 Msa0135980:intron 45.0%
GTAGGGAAAATCCTCATACC+AGG - chr1_3:67773571-67773590 Msa0135990:intron 45.0%
TTGTTTAGCAGCTGGTATGG+AGG - chr1_3:67773742-67773761 Msa0135990:intron 45.0%
GGTGGGAAAAAACTTTACGG+TGG + chr1_3:67774425-67774444 None:intergenic 45.0%
AACGCATGTTCCTTTGAGTC+AGG + chr1_3:67775022-67775041 None:intergenic 45.0%
!! GTTTGGTTTGGTTTGGATGG+TGG + chr1_3:67775596-67775615 None:intergenic 45.0%
!! AGGCTTGGTTTGGTTTGGTT+TGG + chr1_3:67775603-67775622 None:intergenic 45.0%
!! GATAGAGGCTTGGTTTGGTT+TGG + chr1_3:67775608-67775627 None:intergenic 45.0%
! GGATAGATAGAGGCTTGGTT+TGG + chr1_3:67775613-67775632 None:intergenic 45.0%
CAGTTGAAAGCTCTATGCCA+AGG - chr1_3:67775761-67775780 Msa0135990:intron 45.0%
!! TGATTTCGGCCGTTGATTTG+AGG - chr1_3:67775896-67775915 Msa0135990:intron 45.0%
AACGGACACTAATATCACGG+TGG - chr1_3:67776144-67776163 Msa0135990:intron 45.0%
GCTTCTGTTGCTAACACTTG+TGG - chr1_3:67776310-67776329 Msa0135990:intron 45.0%
GTTTGCAAGGAAAGGAAGAG+GGG + chr1_3:67777137-67777156 None:intergenic 45.0%
GGTTTGCAAGGAAAGGAAGA+GGG + chr1_3:67777138-67777157 None:intergenic 45.0%
!! ACAGTTTTGTGTGTGGCAGA+GGG + chr1_3:67777214-67777233 None:intergenic 45.0%
TCAGCATCGTGTTCTCTCAT+AGG + chr1_3:67777418-67777437 None:intergenic 45.0%
TGACTGTGTCAGGTCAATGT+CGG - chr1_3:67777486-67777505 Msa0135990:CDS 45.0%
CTTCTGAATGTATGCGTGTC+TGG + chr1_3:67777515-67777534 None:intergenic 45.0%
! CAGAAGTGATGACGCTACAT+AGG - chr1_3:67777529-67777548 Msa0135990:CDS 45.0%
AGAAGCAATGTCCTTGACTC+AGG - chr1_3:67777595-67777614 Msa0135990:CDS 45.0%
GAAGCAATGTCCTTGACTCA+GGG - chr1_3:67777596-67777615 Msa0135990:CDS 45.0%
CCACCAACATGGAATTTGGA+AGG + chr1_3:67777669-67777688 None:intergenic 45.0%
CCTTCCAAATTCCATGTTGG+TGG - chr1_3:67777666-67777685 Msa0135990:CDS 45.0%
AGCACCACCAACATGGAATT+TGG + chr1_3:67777673-67777692 None:intergenic 45.0%
!! TTTGAGAAGCACCACCAACA+TGG + chr1_3:67777680-67777699 None:intergenic 45.0%
!! TTGGTGGTGCTTCTCAAAGT+TGG - chr1_3:67777682-67777701 Msa0135990:CDS 45.0%
! TGGTGGTGCTTCTCAAAGTT+GGG - chr1_3:67777683-67777702 Msa0135990:CDS 45.0%
GTATGCATCATCCAATGTGC+AGG + chr1_3:67777734-67777753 None:intergenic 45.0%
GAAGAGGCGATGAGTTCTAT+AGG + chr1_3:67777796-67777815 None:intergenic 45.0%
!! GTAGTGGATGTGAGTCAGGT+TGG - chr1_3:67772920-67772939 Msa0135990:three_prime_UTR 50.0%
!! GGTGGAACTGAAGGGTACTA+TGG - chr1_3:67772985-67773004 Msa0135990:three_prime_UTR 50.0%
GAGTCAGGAAGATATGAGGC+AGG + chr1_3:67775007-67775026 None:intergenic 50.0%
TCCAAACAGACAGGCTTGGA+GGG + chr1_3:67775151-67775170 None:intergenic 50.0%
TTCCAAACAGACAGGCTTGG+AGG + chr1_3:67775152-67775171 None:intergenic 50.0%
AGCTTCCAAACAGACAGGCT+TGG + chr1_3:67775155-67775174 None:intergenic 50.0%
GTGACAGCTTCCAAACAGAC+AGG + chr1_3:67775160-67775179 None:intergenic 50.0%
!! GGTTTGGTTTGGATGGTGGT+TGG + chr1_3:67775592-67775611 None:intergenic 50.0%
GCGTATCCTGCTCATGACTT+TGG + chr1_3:67776584-67776603 None:intergenic 50.0%
ACTCGCACATGAGACTAGCA+TGG + chr1_3:67776644-67776663 None:intergenic 50.0%
GTCGGCCAGAGTCATATTTG+AGG - chr1_3:67776772-67776791 Msa0135990:intron 50.0%
GGGTTTGCAAGGAAAGGAAG+AGG + chr1_3:67777139-67777158 None:intergenic 50.0%
AGAAGGGGGTTTGCAAGGAA+AGG + chr1_3:67777145-67777164 None:intergenic 50.0%
TTCGTAGAAGGGGGTTTGCA+AGG + chr1_3:67777150-67777169 None:intergenic 50.0%
!! CACAGTTTTGTGTGTGGCAG+AGG + chr1_3:67777215-67777234 None:intergenic 50.0%
TCCCTCCAAGCCTGTCTGTT+TGG - chr1_3:67775147-67775166 Msa0135990:intron 55.0%
CAACGCAAGACCCTGAGTCA+AGG + chr1_3:67777609-67777628 None:intergenic 55.0%
TGACTCAGGGTCTTGCGTTG+CGG - chr1_3:67777609-67777628 Msa0135990:CDS 55.0%
AGGGTCTTGCGTTGCGGTTG+CGG - chr1_3:67777615-67777634 Msa0135990:CDS 60.0%
TGCGGTTGCGGCGATAACAG+TGG - chr1_3:67777627-67777646 Msa0135990:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 67772831 67777859 67772831 ID=Msa0135990;Name=Msa0135990
chr1_3 mRNA 67772831 67777859 67772831 ID=Msa0135990-mRNA-1;Parent=Msa0135990;Name=Msa0135990-mRNA-1;_AED=0.14;_eAED=0.14;_QI=64|1|1|1|0.75|0.6|5|401|191
chr1_3 exon 67772831 67773185 67772831 ID=Msa0135990-mRNA-1:exon:23314;Parent=Msa0135990-mRNA-1
chr1_3 exon 67776861 67776937 67776861 ID=Msa0135990-mRNA-1:exon:23313;Parent=Msa0135990-mRNA-1
chr1_3 exon 67777039 67777115 67777039 ID=Msa0135990-mRNA-1:exon:23312;Parent=Msa0135990-mRNA-1
chr1_3 exon 67777205 67777291 67777205 ID=Msa0135990-mRNA-1:exon:23311;Parent=Msa0135990-mRNA-1
chr1_3 exon 67777415 67777859 67777415 ID=Msa0135990-mRNA-1:exon:23310;Parent=Msa0135990-mRNA-1
chr1_3 five_prime_UTR 67777796 67777859 67777796 ID=Msa0135990-mRNA-1:five_prime_utr;Parent=Msa0135990-mRNA-1
chr1_3 CDS 67777415 67777795 67777415 ID=Msa0135990-mRNA-1:cds;Parent=Msa0135990-mRNA-1
chr1_3 CDS 67777205 67777291 67777205 ID=Msa0135990-mRNA-1:cds;Parent=Msa0135990-mRNA-1
chr1_3 CDS 67777039 67777115 67777039 ID=Msa0135990-mRNA-1:cds;Parent=Msa0135990-mRNA-1
chr1_3 CDS 67776907 67776937 67776907 ID=Msa0135990-mRNA-1:cds;Parent=Msa0135990-mRNA-1
chr1_3 three_prime_UTR 67776861 67776906 67776861 ID=Msa0135990-mRNA-1:three_prime_utr;Parent=Msa0135990-mRNA-1
chr1_3 three_prime_UTR 67772831 67773185 67772831 ID=Msa0135990-mRNA-1:three_prime_utr;Parent=Msa0135990-mRNA-1
Gene Sequence

>Msa0135990

ATGAGTGCATTTGATTCTGAATGTAGTAGTGGATGTGAGTCAGGTTGGACTCTATACCTTGAACATTCTTATGAAAACACTAGATTCATAGGTGGAACTGAAGGGTACTATGGAAACCAACACAGAGACAAAAGAGTCACAAATGAGTATTCTGAAGGAGTGGAAGATTTGTCTATGGTTTCTGATGCTTCTTCTGGTCCTCCACATCTTCCACATGATGATGTTTACTTCAATGAGAAAGACAATGTAGTGAAAAAGCACAAGAAAGGTACAAAGAAACAGAAAGTTGGAGAAAATAAACAACATGATGTTGAAGATCAACAACACCTTCCTTCTTTTCTTCATGACACTGCTAGCTCTCATGTCTTTGACTTTTCAACTACCAATGTTGTTGGGACAAACCAACAAAGTTATGTAGGAAATATGGTAGACTATTCACAAGGTTTTTCAGCAACTAATTATTTTGAGGGAAAATCCTCATACCAGGAAGAACAATTTGGTTTTTTACAACCATCTCTCTCAAAAAATGAACTTCAAGGAAACAACTGGTATGGAGGAAAAAATCAGTTCTATTAA
Protein sequence

>Msa0135990

MSAFDSECSSGCESGWTLYLEHSYENTRFIGGTEGYYGNQHRDKRVTNEYSEGVEDLSMVSDASSGPPHLPHDDVYFNEKDNVVKKHKKGTKKQKVGENKQHDVEDQQHLPSFLHDTASSHVFDFSTTNVVGTNQQSYVGNMVDYSQGFSATNYFEGKSSYQEEQFGFLQPSLSKNELQGNNWYGGKNQFY*