AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0089200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0089200 MtrunA17_Chr1g0207011 92.857 196 9 4 1 191 3 198 7.87e-119 335
Msa0089200 MtrunA17_Chr5g0393821 51.020 196 71 11 1 187 1 180 2.04e-42 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0089200 AT4G33800.2 39.535 172 81 8 9 169 5 164 2.38e-21 86.3
Msa0089200 AT4G33800.1 39.535 172 81 8 9 169 5 164 2.38e-21 86.3

Find 54 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACATGTTCATTATCCTTT+AGG 0.222495 1_2:-70639375 Msa0089210:five_prime_UTR
ATGGTTTCTGATGCTTCTTC+TGG 0.266605 1_2:+70634647 Msa0089200:CDS
AGCACCACCAACATGGAATT+TGG 0.289275 1_2:-70639202 Msa0089210:CDS
TCATACCAGGAAGAACAATT+TGG 0.315479 1_2:+70635162 Msa0089200:CDS
ACAAAGTTATGTAGGAAATA+TGG 0.330154 1_2:+70635000 Msa0089200:CDS
TATGAAAACACTAGATTCAT+AGG 0.343147 1_2:+70634542 Msa0089200:CDS
GAAGAGGCGATGAGTTCTAT+AGG 0.348824 1_2:-70639325 Msa0089210:exon
TATAGGGCAAACCATGTTAA+TGG 0.362210 1_2:-70639308 Msa0089210:CDS
TATTTCCTACATAACTTTGT+TGG 0.375448 1_2:-70634997 None:intergenic
TGTAGTAGTGGATGTGAGTC+AGG 0.376527 1_2:+70634494 Msa0089200:CDS
CTTCTGAATGTATGCGTGTC+TGG 0.391835 1_2:-70639044 Msa0089210:intron
AGGAGTGGAAGATTTGTCTA+TGG 0.397750 1_2:+70634628 Msa0089200:CDS
AAATTGTTCTTCCTGGTATG+AGG 0.406008 1_2:-70635160 None:intergenic
GCTTAATAAACTTATCCTAA+AGG 0.408244 1_2:+70639360 Msa0089200:three_prime_UTR
TCTTTGTTTAGCAGCTGGTA+TGG 0.422441 1_2:+70635317 Msa0089200:intron
GGTGGAACTGAAGGGTACTA+TGG 0.434994 1_2:+70634563 Msa0089200:CDS
CCTTCCAAATTCCATGTTGG+TGG 0.438290 1_2:+70639198 Msa0089200:three_prime_UTR
AGGGTCTTGCGTTGCGGTTG+CGG 0.451542 1_2:+70639147 Msa0089200:three_prime_UTR
AAGAGGCGATGAGTTCTATA+GGG 0.479187 1_2:-70639324 Msa0089210:exon
CAGAAGTGATGACGCTACAT+AGG 0.486769 1_2:+70639061 Msa0089200:three_prime_UTR
GCAGTGTCATGAAGAAAAGA+AGG 0.507146 1_2:-70634803 None:intergenic
GGTACAAAGAAACAGAAAGT+TGG 0.512053 1_2:+70634740 Msa0089200:CDS
ACAAACCAACAAAGTTATGT+AGG 0.515352 1_2:+70634992 Msa0089200:CDS
TTGGTTTGTCCCAACAACAT+TGG 0.519643 1_2:-70634978 None:intergenic
TGCTATTGAAGTTGTTGCAG+TGG 0.524195 1_2:+70635372 Msa0089200:three_prime_UTR
TCTCCTTCCAAATTCCATGT+TGG 0.526459 1_2:+70639195 Msa0089200:three_prime_UTR
AGAAGCAATATCCTTGACTC+AGG 0.533694 1_2:+70639127 Msa0089200:three_prime_UTR
GTTAATGGCACTAACAGTTA+CGG 0.549686 1_2:-70639293 Msa0089210:CDS
AACTGTTAGTGCCATTAACA+TGG 0.549756 1_2:+70639297 Msa0089200:three_prime_UTR
GTATGCATCATCCAATGTGC+AGG 0.549946 1_2:-70639263 Msa0089210:CDS
AGAAGTGATGACGCTACATA+GGG 0.557585 1_2:+70639062 Msa0089200:three_prime_UTR
TGACTCAGGGTCTTGCGTTG+CGG 0.557938 1_2:+70639141 Msa0089200:three_prime_UTR
ACATCATCATGTGGAAGATG+TGG 0.558107 1_2:-70634674 None:intergenic
AGATTCATAGGTGGAACTGA+AGG 0.568350 1_2:+70634554 Msa0089200:CDS
GTCACAAATGAGTATTCTGA+AGG 0.574749 1_2:+70634608 Msa0089200:CDS
AACATGGAATTTGGAAGGAG+AGG 0.586422 1_2:-70639193 Msa0089210:CDS
ATTATGAACTGCCTGCACAT+TGG 0.588435 1_2:+70639252 Msa0089200:three_prime_UTR
AAATGAGTATTCTGAAGGAG+TGG 0.595088 1_2:+70634613 Msa0089200:CDS
TTGTTTAGCAGCTGGTATGG+AGG 0.596105 1_2:+70635320 Msa0089200:intron
TTTGATTCTGAATGTAGTAG+TGG 0.600879 1_2:+70634482 Msa0089200:CDS
CCACCAACATGGAATTTGGA+AGG 0.619705 1_2:-70639198 Msa0089210:CDS
GTAGGGAAAATCCTCATACC+AGG 0.622430 1_2:+70635149 Msa0089200:intron
TGCGGTTGCGGCGATAACAG+TGG 0.623047 1_2:+70639159 Msa0089200:three_prime_UTR
TTTGAGAAGCACCACCAACA+TGG 0.627251 1_2:-70639209 Msa0089210:CDS
CAACGCAAGACCCTGAGTCA+AGG 0.631019 1_2:-70639138 Msa0089210:CDS
TGCAACAACTTCAATAGCAG+AGG 0.636583 1_2:-70635368 None:intergenic
TCATCATGTGGAAGATGTGG+AGG 0.636849 1_2:-70634671 None:intergenic
TTGAAGTAAACATCATCATG+TGG 0.637487 1_2:-70634683 None:intergenic
GATTCATAGGTGGAACTGAA+GGG 0.638366 1_2:+70634555 Msa0089200:CDS
GTAGTGGATGTGAGTCAGGT+TGG 0.642732 1_2:+70634498 Msa0089200:CDS
GAAGCAATATCCTTGACTCA+GGG 0.656361 1_2:+70639128 Msa0089200:three_prime_UTR
TGTCATGAAGAAAAGAAGGA+AGG 0.667075 1_2:-70634799 None:intergenic
GAAAACACTAGATTCATAGG+TGG 0.672436 1_2:+70634545 Msa0089200:CDS
AATATGGTAGACTATTCACA+AGG 0.724521 1_2:+70635016 Msa0089200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAGATATTGAAATTATA+TGG + chr1_2:70635777-70635796 Msa0089200:intron 10.0%
!! AAAAGAATTAATACAATTAA+GGG - chr1_2:70636024-70636043 None:intergenic 10.0%
!! AAAAAGAATTAATACAATTA+AGG - chr1_2:70636025-70636044 None:intergenic 10.0%
!!! TATAAACAAAATCATTTTTT+AGG + chr1_2:70636229-70636248 Msa0089200:intron 10.0%
!! AAATTATAAATCATATACAT+TGG + chr1_2:70636279-70636298 Msa0089200:intron 10.0%
!! AATTACTTATTTAATTAAAC+AGG - chr1_2:70636828-70636847 None:intergenic 10.0%
!!! ATATCATATGTTTTAATTAA+TGG - chr1_2:70638010-70638029 None:intergenic 10.0%
!!! TTTTAAAAAGATGATATATA+TGG - chr1_2:70638269-70638288 None:intergenic 10.0%
!! CATGTGATTATATAAAAAAT+CGG + chr1_2:70636160-70636179 Msa0089200:intron 15.0%
!! TCACTAATAACTTATTTATT+TGG + chr1_2:70636253-70636272 Msa0089200:intron 15.0%
!! AAAGGAATAAAAAAAATCAA+AGG - chr1_2:70636787-70636806 None:intergenic 15.0%
!!! TATACTATAATCTAAAGTTT+TGG + chr1_2:70636951-70636970 Msa0089200:intron 15.0%
!!! AATATTCCTATACAATTTTA+TGG - chr1_2:70637115-70637134 None:intergenic 15.0%
!! AAATTGTATAGGAATATTAA+AGG + chr1_2:70637117-70637136 Msa0089200:intron 15.0%
!! AATTGTATAGGAATATTAAA+GGG + chr1_2:70637118-70637137 Msa0089200:intron 15.0%
!!! TCTTTTTAAAAGTTTTTCAT+AGG + chr1_2:70638278-70638297 Msa0089200:intron 15.0%
!!! ACTAATTATTTTGAGGTAAT+TGG + chr1_2:70635049-70635068 Msa0089200:intron 20.0%
!!! AATTTGTTTGTGTTATTTGT+AGG + chr1_2:70635131-70635150 Msa0089200:intron 20.0%
!!! ATTTGTTTGTGTTATTTGTA+GGG + chr1_2:70635132-70635151 Msa0089200:intron 20.0%
!! TAATTGATAAATTAATCCGA+AGG - chr1_2:70635818-70635837 None:intergenic 20.0%
!!! GTGTATGTACTTTTATTTTT+TGG - chr1_2:70635889-70635908 None:intergenic 20.0%
!! CCATATATTATAACATTTGT+CGG - chr1_2:70636078-70636097 None:intergenic 20.0%
!! CAAAAAAAACTAAACGAAAT+GGG - chr1_2:70636629-70636648 None:intergenic 20.0%
!! ACAAAAAAAACTAAACGAAA+TGG - chr1_2:70636630-70636649 None:intergenic 20.0%
!!! AGCTTCTAAAAAAATTATTG+TGG + chr1_2:70637718-70637737 Msa0089200:intron 20.0%
!!! GCTTCTAAAAAAATTATTGT+GGG + chr1_2:70637719-70637738 Msa0089200:intron 20.0%
!! AAACTAATAACATGTACAAT+TGG + chr1_2:70637878-70637897 Msa0089200:intron 20.0%
!!! TCATATGTTTTAATTAATGG+AGG - chr1_2:70638007-70638026 None:intergenic 20.0%
!!! GTAAATTTTGATTTTCTGTT+GGG - chr1_2:70638539-70638558 Msa0089210:three_prime_UTR 20.0%
!!! TGTAAATTTTGATTTTCTGT+TGG - chr1_2:70638540-70638559 Msa0089210:three_prime_UTR 20.0%
!! AAAATCAAAATTTACAAGCT+AGG + chr1_2:70638544-70638563 Msa0089200:intron 20.0%
!! TGTTTTCATAAGAATGTTCA+AGG - chr1_2:70634532-70634551 None:intergenic 25.0%
! TATGAAAACACTAGATTCAT+AGG + chr1_2:70634542-70634561 Msa0089200:CDS 25.0%
!! CTTTTCAACTGTAAGAAATA+TGG + chr1_2:70634844-70634863 Msa0089200:intron 25.0%
! TATTTCCTACATAACTTTGT+TGG - chr1_2:70635000-70635019 None:intergenic 25.0%
! ACAAAGTTATGTAGGAAATA+TGG + chr1_2:70635000-70635019 Msa0089200:CDS 25.0%
!!! TTCAGCAACTAATTATTTTG+AGG + chr1_2:70635042-70635061 Msa0089200:CDS 25.0%
! TGTTACTCAAAGATTAATGA+AGG + chr1_2:70635396-70635415 Msa0089200:intron 25.0%
! ACTGAATTGACAAAGTAAAA+AGG - chr1_2:70635439-70635458 None:intergenic 25.0%
!! TTTTTACTTTGTCAATTCAG+TGG + chr1_2:70635438-70635457 Msa0089200:intron 25.0%
!!! GTAATTTTGTTGTTAATCGA+TGG + chr1_2:70635509-70635528 Msa0089200:intron 25.0%
! GATTACAAGCTAATTTAGTT+AGG + chr1_2:70635533-70635552 Msa0089200:intron 25.0%
! GTAGATTCATCAAAATAACA+AGG - chr1_2:70635584-70635603 None:intergenic 25.0%
! TTTCAGTACATTAAGATGTA+CGG - chr1_2:70635934-70635953 None:intergenic 25.0%
! GAATTAATACAATTAAGGGT+GGG - chr1_2:70636020-70636039 None:intergenic 25.0%
! AGAATTAATACAATTAAGGG+TGG - chr1_2:70636021-70636040 None:intergenic 25.0%
! CCGACAAATGTTATAATATA+TGG + chr1_2:70636075-70636094 Msa0089200:intron 25.0%
! TAGCTTATAAACGAATCTAA+AGG - chr1_2:70636335-70636354 None:intergenic 25.0%
! AATCAGATTACATGCATATT+TGG - chr1_2:70636419-70636438 None:intergenic 25.0%
!! TGATTCTAGAAAAGAGAATA+TGG + chr1_2:70636434-70636453 Msa0089200:intron 25.0%
!! TTTGTCAACTTTATCTCTTT+TGG + chr1_2:70636645-70636664 Msa0089200:intron 25.0%
!! TTGTCAACTTTATCTCTTTT+GGG + chr1_2:70636646-70636665 Msa0089200:intron 25.0%
! AATTGACTAGGAATAAGTAA+AGG - chr1_2:70636805-70636824 None:intergenic 25.0%
! TAATTAAACAGGAATTGACT+AGG - chr1_2:70636817-70636836 None:intergenic 25.0%
!!! CTTAACTTTTTCAACCTTTA+TGG - chr1_2:70636867-70636886 None:intergenic 25.0%
! CATAAAGGTTGAAAAAGTTA+AGG + chr1_2:70636865-70636884 Msa0089200:intron 25.0%
!! AATATGACCTTTGAGAATTT+AGG + chr1_2:70636890-70636909 Msa0089200:intron 25.0%
! AAAAACCTAAGTAAAGAAGA+AGG + chr1_2:70637000-70637019 Msa0089200:intron 25.0%
! TGCAATCCATAAAATTGTAT+AGG + chr1_2:70637106-70637125 Msa0089200:intron 25.0%
! TATAGGAATATTAAAGGGTA+TGG + chr1_2:70637123-70637142 Msa0089200:intron 25.0%
!! ATAGGAATATTAAAGGGTAT+GGG + chr1_2:70637124-70637143 Msa0089200:intron 25.0%
! ATAAAGAGGATATCAGAAAA+TGG - chr1_2:70637254-70637273 None:intergenic 25.0%
! AACATTTAACCTCAAATCAA+CGG - chr1_2:70637485-70637504 None:intergenic 25.0%
! TATCCAATTTCCAATTTCAT+TGG - chr1_2:70637923-70637942 None:intergenic 25.0%
!!! TGTTTTAATTAATGGAGGAT+TGG - chr1_2:70638002-70638021 None:intergenic 25.0%
!!! GTTTTGAAATAGAGTTCAAA+AGG + chr1_2:70638041-70638060 Msa0089200:intron 25.0%
! AGATTACCAAATAGAAAAGA+GGG - chr1_2:70638461-70638480 Msa0089210:three_prime_UTR 25.0%
! TAGATTACCAAATAGAAAAG+AGG - chr1_2:70638462-70638481 Msa0089210:three_prime_UTR 25.0%
! TTATTACAAGCGTAATTACA+AGG - chr1_2:70638717-70638736 Msa0089210:CDS 25.0%
! AGAATATTTGAAGAAAACAG+AGG - chr1_2:70638855-70638874 Msa0089210:intron 25.0%
! GCTTAATAAACTTATCCTAA+AGG + chr1_2:70639360-70639379 Msa0089200:three_prime_UTR 25.0%
TTTGATTCTGAATGTAGTAG+TGG + chr1_2:70634482-70634501 Msa0089200:CDS 30.0%
TTGAAGTAAACATCATCATG+TGG - chr1_2:70634686-70634705 None:intergenic 30.0%
!! ATTTTTCAGACCAATGTTGT+TGG + chr1_2:70634968-70634987 Msa0089200:intron 30.0%
!! TTTTTCAGACCAATGTTGTT+GGG + chr1_2:70634969-70634988 Msa0089200:intron 30.0%
ACAAACCAACAAAGTTATGT+AGG + chr1_2:70634992-70635011 Msa0089200:CDS 30.0%
AATATGGTAGACTATTCACA+AGG + chr1_2:70635016-70635035 Msa0089200:CDS 30.0%
AAAAACCAAATTGTTCTTCC+TGG - chr1_2:70635170-70635189 None:intergenic 30.0%
!!! AGTTCATTTTTTGAGAGAGA+TGG - chr1_2:70635198-70635217 None:intergenic 30.0%
CTCTCAAAAAATGAACTTCA+AGG + chr1_2:70635201-70635220 Msa0089200:CDS 30.0%
ACAAGCTAATTTAGTTAGGA+AGG + chr1_2:70635537-70635556 Msa0089200:intron 30.0%
! AGTAGAACAGATTTTGAATG+AGG - chr1_2:70635719-70635738 None:intergenic 30.0%
GTTGTATATTACTCAACCTT+CGG + chr1_2:70635799-70635818 Msa0089200:intron 30.0%
! AATTTAGTCACACACTGATT+AGG + chr1_2:70636126-70636145 Msa0089200:intron 30.0%
GGAATTTGACAAAATTACGA+GGG - chr1_2:70636388-70636407 None:intergenic 30.0%
AGGAATTTGACAAAATTACG+AGG - chr1_2:70636389-70636408 None:intergenic 30.0%
! CGTAATTTTGTCAAATTCCT+AGG + chr1_2:70636389-70636408 Msa0089200:intron 30.0%
!! ATATGGCAAAAGTGTTATGA+AGG + chr1_2:70636451-70636470 Msa0089200:intron 30.0%
!! TATGGCAAAAGTGTTATGAA+GGG + chr1_2:70636452-70636471 Msa0089200:intron 30.0%
GACAGTATACAAATATGATG+AGG + chr1_2:70636487-70636506 Msa0089200:intron 30.0%
AGTATACAAATATGATGAGG+CGG + chr1_2:70636490-70636509 Msa0089200:intron 30.0%
TTCCATTCCAACATCAATAT+TGG - chr1_2:70636521-70636540 None:intergenic 30.0%
TTGTCATCCTAAATTCTCAA+AGG - chr1_2:70636900-70636919 None:intergenic 30.0%
TAGTATATGAGAAAACACGT+GGG - chr1_2:70636938-70636957 None:intergenic 30.0%
ATAGTATATGAGAAAACACG+TGG - chr1_2:70636939-70636958 None:intergenic 30.0%
GTTAACCTTCTTCTTTACTT+AGG - chr1_2:70637008-70637027 None:intergenic 30.0%
! TACGTGTTTGATTTCTCTTT+GGG - chr1_2:70637030-70637049 None:intergenic 30.0%
! TTACGTGTTTGATTTCTCTT+TGG - chr1_2:70637031-70637050 None:intergenic 30.0%
ATTACAACAAGGATAGATAG+AGG - chr1_2:70637200-70637219 None:intergenic 30.0%
CCTCTATTACAATTACAACA+AGG - chr1_2:70637211-70637230 None:intergenic 30.0%
! CCTTGTTGTAATTGTAATAG+AGG + chr1_2:70637208-70637227 Msa0089200:intron 30.0%
TGTTGTAATTGTAATAGAGG+AGG + chr1_2:70637211-70637230 Msa0089200:intron 30.0%
! TCAGAAAATGGACAAGTTTT+TGG - chr1_2:70637242-70637261 None:intergenic 30.0%
AAAGTTATGTCAAGGGAATT+GGG - chr1_2:70637306-70637325 None:intergenic 30.0%
AAAAGTTATGTCAAGGGAAT+TGG - chr1_2:70637307-70637326 None:intergenic 30.0%
TCTCATGCTGTAAATGATTT+CGG + chr1_2:70637459-70637478 Msa0089200:intron 30.0%
!! AGCTCAACAATAGTTCTAAA+CGG + chr1_2:70637659-70637678 Msa0089200:intron 30.0%
!! GGTGGATTAGAATCTATAAT+TGG + chr1_2:70637695-70637714 Msa0089200:intron 30.0%
! CTTAAAAGCACAAAACAAGA+TGG + chr1_2:70638075-70638094 Msa0089200:intron 30.0%
TAATGGTAGATCTCATTTGA+TGG + chr1_2:70638151-70638170 Msa0089200:intron 30.0%
CTATTACTTTCATGCATTCT+TGG - chr1_2:70638241-70638260 None:intergenic 30.0%
! AAGTTTTTCATAGGTTTCGT+CGG + chr1_2:70638287-70638306 Msa0089200:intron 30.0%
ATGGTCAACTAAAGAAGATT+AGG - chr1_2:70638413-70638432 Msa0089210:three_prime_UTR 30.0%
TTGACCATACATATCTTACT+AGG + chr1_2:70638425-70638444 Msa0089200:intron 30.0%
TTTGCCTAGTAAGATATGTA+TGG - chr1_2:70638432-70638451 Msa0089210:three_prime_UTR 30.0%
! TGATTTTCTGTTGGGAAATT+CGG - chr1_2:70638531-70638550 Msa0089210:three_prime_UTR 30.0%
ATCAAAATTTACAAGCTAGG+TGG + chr1_2:70638547-70638566 Msa0089200:intron 30.0%
! ATTACTTTGAGTTCGTAGAA+GGG - chr1_2:70638694-70638713 Msa0089210:CDS 30.0%
!! TTTAATCACAGTTTTGTGTG+TGG - chr1_2:70638754-70638773 Msa0089210:intron 30.0%
AAGCAAACTAAAAAGAGAAG+AGG - chr1_2:70639344-70639363 Msa0089210:five_prime_UTR 30.0%
GAAAACACTAGATTCATAGG+TGG + chr1_2:70634545-70634564 Msa0089200:CDS 35.0%
! TGACTCTTTTGTCTTTGTGT+TGG - chr1_2:70634592-70634611 None:intergenic 35.0%
GTCACAAATGAGTATTCTGA+AGG + chr1_2:70634608-70634627 Msa0089200:CDS 35.0%
! AAATGAGTATTCTGAAGGAG+TGG + chr1_2:70634613-70634632 Msa0089200:CDS 35.0%
! GTAGTGAAAAAGCACAAGAA+AGG + chr1_2:70634719-70634738 Msa0089200:CDS 35.0%
GGTACAAAGAAACAGAAAGT+TGG + chr1_2:70634740-70634759 Msa0089200:CDS 35.0%
TGTCATGAAGAAAAGAAGGA+AGG - chr1_2:70634802-70634821 None:intergenic 35.0%
AAATTGTTCTTCCTGGTATG+AGG - chr1_2:70635163-70635182 None:intergenic 35.0%
TCATACCAGGAAGAACAATT+TGG + chr1_2:70635162-70635181 Msa0089200:CDS 35.0%
! TTCATTCTTTGTTTAGCAGC+TGG + chr1_2:70635312-70635331 Msa0089200:intron 35.0%
CACAATCTAAATCCATGGAA+TGG + chr1_2:70635852-70635871 Msa0089200:intron 35.0%
GAACATATACTTCCATTCCA+TGG - chr1_2:70635867-70635886 None:intergenic 35.0%
AAGGGTGGGAAAAAACTTTA+CGG - chr1_2:70636006-70636025 None:intergenic 35.0%
!! CAAAAGTGTTATGAAGGGAT+GGG + chr1_2:70636457-70636476 Msa0089200:intron 35.0%
GGAAATGCCAATATTGATGT+TGG + chr1_2:70636511-70636530 Msa0089200:intron 35.0%
TGCCAATATTGATGTTGGAA+TGG + chr1_2:70636516-70636535 Msa0089200:intron 35.0%
! TCAACTTTATCTCTTTTGGG+AGG + chr1_2:70636649-70636668 Msa0089200:intron 35.0%
!!! CTTTTTCAACCTTTATGGAC+TGG - chr1_2:70636862-70636881 None:intergenic 35.0%
AGTATATGAGAAAACACGTG+GGG - chr1_2:70636937-70636956 None:intergenic 35.0%
!! CAATTTTATGGATTGCAGCT+AGG - chr1_2:70637103-70637122 None:intergenic 35.0%
TGACCATTGCCAATTTAGTT+TGG + chr1_2:70637275-70637294 Msa0089200:intron 35.0%
CATCCAAACTAAATTGGCAA+TGG - chr1_2:70637281-70637300 None:intergenic 35.0%
TGGGAACATCCAAACTAAAT+TGG - chr1_2:70637287-70637306 None:intergenic 35.0%
GTGCACAAAAGTTATGTCAA+GGG - chr1_2:70637313-70637332 None:intergenic 35.0%
AGTGCACAAAAGTTATGTCA+AGG - chr1_2:70637314-70637333 None:intergenic 35.0%
GTGTAGCACATTTATTTCCT+TGG - chr1_2:70637358-70637377 None:intergenic 35.0%
! TTTGGTGTGACATAATGAGT+TGG - chr1_2:70637406-70637425 None:intergenic 35.0%
CATTATGTCACACCAAAAAC+TGG + chr1_2:70637409-70637428 Msa0089200:intron 35.0%
TATTGTTACACCATGCAGTA+GGG - chr1_2:70637565-70637584 None:intergenic 35.0%
TTATTGTTACACCATGCAGT+AGG - chr1_2:70637566-70637585 None:intergenic 35.0%
CTAAACGGACACTAATATCA+CGG + chr1_2:70637674-70637693 Msa0089200:intron 35.0%
TGTGGGTAAACACTAACTTT+AGG + chr1_2:70637736-70637755 Msa0089200:intron 35.0%
TAAACACTAACTTTAGGTGG+TGG + chr1_2:70637742-70637761 Msa0089200:intron 35.0%
CTTCACGTTTAGTCAATAGT+CGG + chr1_2:70637774-70637793 Msa0089200:intron 35.0%
GTGCCAATGAAATTGGAAAT+TGG + chr1_2:70637917-70637936 Msa0089200:intron 35.0%
CTCATGACTTTGGATCAATT+TGG - chr1_2:70638107-70638126 None:intergenic 35.0%
! GATCTACCATTATGTCACTT+AGG - chr1_2:70638143-70638162 None:intergenic 35.0%
!! ATGAAAAGTGTTAGACTTCG+AGG - chr1_2:70638386-70638405 None:intergenic 35.0%
! AACCATCCCTCTTTTCTATT+TGG + chr1_2:70638452-70638471 Msa0089200:intron 35.0%
TACCAAATAGAAAAGAGGGA+TGG - chr1_2:70638457-70638476 Msa0089210:three_prime_UTR 35.0%
!! TGTTAGCTTTAAGCCAATCA+AGG + chr1_2:70638618-70638637 Msa0089200:intron 35.0%
AAGCTTTCAGAAGCAGAAAA+GGG - chr1_2:70638647-70638666 Msa0089210:CDS 35.0%
! TTACTTTGAGTTCGTAGAAG+GGG - chr1_2:70638693-70638712 Msa0089210:CDS 35.0%
! GATTACTTTGAGTTCGTAGA+AGG - chr1_2:70638695-70638714 Msa0089210:CDS 35.0%
AAATGACAGAGAGGTAAGTA+GGG - chr1_2:70638819-70638838 Msa0089210:intron 35.0%
AAAATGACAGAGAGGTAAGT+AGG - chr1_2:70638820-70638839 Msa0089210:intron 35.0%
GAAGAAAACAGAGGAAAACA+AGG - chr1_2:70638846-70638865 Msa0089210:CDS 35.0%
!!! CGACATGAACATGTTTTTAG+TGG - chr1_2:70638923-70638942 Msa0089210:intron 35.0%
TAATCAAATGTGACTGTGTC+AGG + chr1_2:70639008-70639027 Msa0089200:intron 35.0%
GTTAATGGCACTAACAGTTA+CGG - chr1_2:70639296-70639315 Msa0089210:CDS 35.0%
! AACTGTTAGTGCCATTAACA+TGG + chr1_2:70639297-70639316 Msa0089200:three_prime_UTR 35.0%
TATAGGGCAAACCATGTTAA+TGG - chr1_2:70639311-70639330 Msa0089210:CDS 35.0%
AGATTCATAGGTGGAACTGA+AGG + chr1_2:70634554-70634573 Msa0089200:CDS 40.0%
GATTCATAGGTGGAACTGAA+GGG + chr1_2:70634555-70634574 Msa0089200:CDS 40.0%
AGGAGTGGAAGATTTGTCTA+TGG + chr1_2:70634628-70634647 Msa0089200:CDS 40.0%
ATGGTTTCTGATGCTTCTTC+TGG + chr1_2:70634647-70634666 Msa0089200:CDS 40.0%
ACATCATCATGTGGAAGATG+TGG - chr1_2:70634677-70634696 None:intergenic 40.0%
GCAGTGTCATGAAGAAAAGA+AGG - chr1_2:70634806-70634825 None:intergenic 40.0%
!! TTGGTTTGTCCCAACAACAT+TGG - chr1_2:70634981-70635000 None:intergenic 40.0%
! TCTTTGTTTAGCAGCTGGTA+TGG + chr1_2:70635317-70635336 Msa0089200:intron 40.0%
TGCAACAACTTCAATAGCAG+AGG - chr1_2:70635371-70635390 None:intergenic 40.0%
TGCTATTGAAGTTGTTGCAG+TGG + chr1_2:70635372-70635391 Msa0089200:three_prime_UTR 40.0%
GCATGCACAATCTAAATCCA+TGG + chr1_2:70635847-70635866 Msa0089200:intron 40.0%
! TAGTCACACACTGATTAGGT+AGG + chr1_2:70636130-70636149 Msa0089200:intron 40.0%
CATGCATATTTGGATTGCCT+AGG - chr1_2:70636409-70636428 None:intergenic 40.0%
!! GCAAAAGTGTTATGAAGGGA+TGG + chr1_2:70636456-70636475 Msa0089200:intron 40.0%
CTTTGAGTCAGGAAGATATG+AGG - chr1_2:70636589-70636608 None:intergenic 40.0%
CTCATATCTTCCTGACTCAA+AGG + chr1_2:70636587-70636606 Msa0089200:intron 40.0%
ATTCTGCTACCAGTCCATAA+AGG + chr1_2:70636850-70636869 Msa0089200:intron 40.0%
!! TTGGTTTGGTTTGGTTTGGA+TGG - chr1_2:70637176-70637195 None:intergenic 40.0%
AACAAGGATAGATAGAGGCT+TGG - chr1_2:70637195-70637214 None:intergenic 40.0%
TTGGCAATGGTCACATAAAG+AGG - chr1_2:70637268-70637287 None:intergenic 40.0%
! GCATATCTCGATCCAGTTTT+TGG - chr1_2:70637424-70637443 None:intergenic 40.0%
! AAAAGTTGATCCCTACTGCA+TGG + chr1_2:70637552-70637571 Msa0089200:intron 40.0%
AGAACTATTGTTGAGCTGCA+CGG - chr1_2:70637656-70637675 None:intergenic 40.0%
GGGTAAACACTAACTTTAGG+TGG + chr1_2:70637739-70637758 Msa0089200:intron 40.0%
TACACGAGTGCCAATGAAAT+TGG + chr1_2:70637910-70637929 Msa0089200:intron 40.0%
ATTGATCCAAAGTCATGAGC+AGG + chr1_2:70638108-70638127 Msa0089200:intron 40.0%
CGCAAACCTAAGTGACATAA+TGG + chr1_2:70638134-70638153 Msa0089200:intron 40.0%
CATTGCCTCAAATATGACTC+TGG - chr1_2:70638313-70638332 None:intergenic 40.0%
GGTACAGTTTCTTTCTTGCA+AGG - chr1_2:70638504-70638523 Msa0089210:three_prime_UTR 40.0%
TCTGTTGGGAAATTCGGATA+TGG - chr1_2:70638525-70638544 Msa0089210:three_prime_UTR 40.0%
!! CAGAAAAGGGTATCCTTGAT+TGG - chr1_2:70638634-70638653 Msa0089210:CDS 40.0%
GAAGCTTTCAGAAGCAGAAA+AGG - chr1_2:70638648-70638667 Msa0089210:CDS 40.0%
! TACTTTGAGTTCGTAGAAGG+GGG - chr1_2:70638692-70638711 Msa0089210:CDS 40.0%
AATGACAGAGAGGTAAGTAG+GGG - chr1_2:70638818-70638837 Msa0089210:intron 40.0%
CAAGGAGAAAAATGACAGAG+AGG - chr1_2:70638828-70638847 Msa0089210:CDS 40.0%
! AGAAGTGATGACGCTACATA+GGG + chr1_2:70639062-70639081 Msa0089200:three_prime_UTR 40.0%
AGAAGCAATATCCTTGACTC+AGG + chr1_2:70639127-70639146 Msa0089200:three_prime_UTR 40.0%
GAAGCAATATCCTTGACTCA+GGG + chr1_2:70639128-70639147 Msa0089200:three_prime_UTR 40.0%
AACATGGAATTTGGAAGGAG+AGG - chr1_2:70639196-70639215 Msa0089210:CDS 40.0%
TCTCCTTCCAAATTCCATGT+TGG + chr1_2:70639195-70639214 Msa0089200:three_prime_UTR 40.0%
ATTATGAACTGCCTGCACAT+TGG + chr1_2:70639252-70639271 Msa0089200:three_prime_UTR 40.0%
AAGAGGCGATGAGTTCTATA+GGG - chr1_2:70639327-70639346 Msa0089210:exon 40.0%
!! TGTAGTAGTGGATGTGAGTC+AGG + chr1_2:70634494-70634513 Msa0089200:CDS 45.0%
TCATCATGTGGAAGATGTGG+AGG - chr1_2:70634674-70634693 None:intergenic 45.0%
GTAGGGAAAATCCTCATACC+AGG + chr1_2:70635149-70635168 Msa0089200:intron 45.0%
TTGTTTAGCAGCTGGTATGG+AGG + chr1_2:70635320-70635339 Msa0089200:intron 45.0%
GGTGGGAAAAAACTTTACGG+TGG - chr1_2:70636003-70636022 None:intergenic 45.0%
AACGCATGTTCCTTTGAGTC+AGG - chr1_2:70636600-70636619 None:intergenic 45.0%
!! GTTTGGTTTGGTTTGGATGG+TGG - chr1_2:70637173-70637192 None:intergenic 45.0%
!! AGGCTTGGTTTGGTTTGGTT+TGG - chr1_2:70637180-70637199 None:intergenic 45.0%
!! GATAGAGGCTTGGTTTGGTT+TGG - chr1_2:70637185-70637204 None:intergenic 45.0%
! GGATAGATAGAGGCTTGGTT+TGG - chr1_2:70637190-70637209 None:intergenic 45.0%
CAGTTGAAAGCTCTATGCCA+AGG + chr1_2:70637338-70637357 Msa0089200:intron 45.0%
!! TGATTTCGGCCGTTGATTTG+AGG + chr1_2:70637473-70637492 Msa0089200:intron 45.0%
AACGGACACTAATATCACGG+TGG + chr1_2:70637677-70637696 Msa0089200:intron 45.0%
GCTTCTGTTGCTAACACTTG+TGG + chr1_2:70637843-70637862 Msa0089200:intron 45.0%
GTTTGCAAGGAAAGGAAGAG+GGG - chr1_2:70638670-70638689 Msa0089210:CDS 45.0%
GGTTTGCAAGGAAAGGAAGA+GGG - chr1_2:70638671-70638690 Msa0089210:CDS 45.0%
!! ACAGTTTTGTGTGTGGCAGA+GGG - chr1_2:70638747-70638766 Msa0089210:intron 45.0%
TCAGCATCGTGTTCTCTCAT+AGG - chr1_2:70638951-70638970 Msa0089210:intron 45.0%
TGACTGTGTCAGGTCAATGT+CGG + chr1_2:70639018-70639037 Msa0089200:intron 45.0%
CTTCTGAATGTATGCGTGTC+TGG - chr1_2:70639047-70639066 Msa0089210:intron 45.0%
! CAGAAGTGATGACGCTACAT+AGG + chr1_2:70639061-70639080 Msa0089200:three_prime_UTR 45.0%
CCACCAACATGGAATTTGGA+AGG - chr1_2:70639201-70639220 Msa0089210:CDS 45.0%
CCTTCCAAATTCCATGTTGG+TGG + chr1_2:70639198-70639217 Msa0089200:three_prime_UTR 45.0%
AGCACCACCAACATGGAATT+TGG - chr1_2:70639205-70639224 Msa0089210:CDS 45.0%
!! TTTGAGAAGCACCACCAACA+TGG - chr1_2:70639212-70639231 Msa0089210:CDS 45.0%
GTATGCATCATCCAATGTGC+AGG - chr1_2:70639266-70639285 Msa0089210:CDS 45.0%
GAAGAGGCGATGAGTTCTAT+AGG - chr1_2:70639328-70639347 Msa0089210:exon 45.0%
!! GTAGTGGATGTGAGTCAGGT+TGG + chr1_2:70634498-70634517 Msa0089200:CDS 50.0%
!! GGTGGAACTGAAGGGTACTA+TGG + chr1_2:70634563-70634582 Msa0089200:CDS 50.0%
GAGTCAGGAAGATATGAGGC+AGG - chr1_2:70636585-70636604 None:intergenic 50.0%
TCCAAACAGACAGGCTTGGA+GGG - chr1_2:70636729-70636748 None:intergenic 50.0%
TTCCAAACAGACAGGCTTGG+AGG - chr1_2:70636730-70636749 None:intergenic 50.0%
AGCTTCCAAACAGACAGGCT+TGG - chr1_2:70636733-70636752 None:intergenic 50.0%
GTGACAGCTTCCAAACAGAC+AGG - chr1_2:70636738-70636757 None:intergenic 50.0%
!! GGTTTGGTTTGGATGGTGGT+TGG - chr1_2:70637169-70637188 None:intergenic 50.0%
GCGTATCCTGCTCATGACTT+TGG - chr1_2:70638117-70638136 None:intergenic 50.0%
ACTCGCACATGAGACTAGCA+TGG - chr1_2:70638177-70638196 None:intergenic 50.0%
GTCGGCCAGAGTCATATTTG+AGG + chr1_2:70638305-70638324 Msa0089200:intron 50.0%
GGGTTTGCAAGGAAAGGAAG+AGG - chr1_2:70638672-70638691 Msa0089210:CDS 50.0%
AGAAGGGGGTTTGCAAGGAA+AGG - chr1_2:70638678-70638697 Msa0089210:CDS 50.0%
TTCGTAGAAGGGGGTTTGCA+AGG - chr1_2:70638683-70638702 Msa0089210:CDS 50.0%
!! CACAGTTTTGTGTGTGGCAG+AGG - chr1_2:70638748-70638767 Msa0089210:intron 50.0%
TCCCTCCAAGCCTGTCTGTT+TGG + chr1_2:70636725-70636744 Msa0089200:intron 55.0%
CAACGCAAGACCCTGAGTCA+AGG - chr1_2:70639141-70639160 Msa0089210:CDS 55.0%
TGACTCAGGGTCTTGCGTTG+CGG + chr1_2:70639141-70639160 Msa0089200:three_prime_UTR 55.0%
AGGGTCTTGCGTTGCGGTTG+CGG + chr1_2:70639147-70639166 Msa0089200:three_prime_UTR 60.0%
TGCGGTTGCGGCGATAACAG+TGG + chr1_2:70639159-70639178 Msa0089200:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 70634409 70639391 70634409 ID=Msa0089200;Name=Msa0089200
chr1_2 mRNA 70634409 70639391 70634409 ID=Msa0089200-mRNA-1;Parent=Msa0089200;Name=Msa0089200-mRNA-1;_AED=0.14;_eAED=0.14;_QI=64|1|1|1|0.75|0.6|5|401|191
chr1_2 exon 70634409 70634853 70634409 ID=Msa0089200-mRNA-1:exon:24290;Parent=Msa0089200-mRNA-1
chr1_2 exon 70634977 70635063 70634977 ID=Msa0089200-mRNA-1:exon:24291;Parent=Msa0089200-mRNA-1
chr1_2 exon 70635153 70635229 70635153 ID=Msa0089200-mRNA-1:exon:24292;Parent=Msa0089200-mRNA-1
chr1_2 exon 70635331 70635407 70635331 ID=Msa0089200-mRNA-1:exon:24293;Parent=Msa0089200-mRNA-1
chr1_2 exon 70639037 70639391 70639037 ID=Msa0089200-mRNA-1:exon:24294;Parent=Msa0089200-mRNA-1
chr1_2 five_prime_UTR 70634409 70634472 70634409 ID=Msa0089200-mRNA-1:five_prime_utr;Parent=Msa0089200-mRNA-1
chr1_2 CDS 70634473 70634853 70634473 ID=Msa0089200-mRNA-1:cds;Parent=Msa0089200-mRNA-1
chr1_2 CDS 70634977 70635063 70634977 ID=Msa0089200-mRNA-1:cds;Parent=Msa0089200-mRNA-1
chr1_2 CDS 70635153 70635229 70635153 ID=Msa0089200-mRNA-1:cds;Parent=Msa0089200-mRNA-1
chr1_2 CDS 70635331 70635361 70635331 ID=Msa0089200-mRNA-1:cds;Parent=Msa0089200-mRNA-1
chr1_2 three_prime_UTR 70635362 70635407 70635362 ID=Msa0089200-mRNA-1:three_prime_utr;Parent=Msa0089200-mRNA-1
chr1_2 three_prime_UTR 70639037 70639391 70639037 ID=Msa0089200-mRNA-1:three_prime_utr;Parent=Msa0089200-mRNA-1
Gene Sequence

>Msa0089200

ATGAGTGCATTTGATTCTGAATGTAGTAGTGGATGTGAGTCAGGTTGGACTCTATACCTTGAACATTCTTATGAAAACACTAGATTCATAGGTGGAACTGAAGGGTACTATGGAAACCAACACAAAGACAAAAGAGTCACAAATGAGTATTCTGAAGGAGTGGAAGATTTGTCTATGGTTTCTGATGCTTCTTCTGGTCCTCCACATCTTCCACATGATGATGTTTACTTCAATGAGAAAGACAATGTAGTGAAAAAGCACAAGAAAGGTACAAAGAAACAGAAAGTTGGAGAAAATAAACAACATGATGTTGAAGATCAACAACACCTTCCTTCTTTTCTTCATGACACTGCTAGCTCTCATGTCTTTGACTTTTCAACTACCAATGTTGTTGGGACAAACCAACAAAGTTATGTAGGAAATATGGTAGACTATTCACAAGGTTTTTCAGCAACTAATTATTTTGAGGGAAAATCCTCATACCAGGAAGAACAATTTGGTTTTTTACAACCATCTCTCTCAAAAAATGAACTTCAAGGAAACAACTGGTATGGAGGAAAAAATCAGTTCTATTAA
Protein sequence

>Msa0089200

MSAFDSECSSGCESGWTLYLEHSYENTRFIGGTEGYYGNQHKDKRVTNEYSEGVEDLSMVSDASSGPPHLPHDDVYFNEKDNVVKKHKKGTKKQKVGENKQHDVEDQQHLPSFLHDTASSHVFDFSTTNVVGTNQQSYVGNMVDYSQGFSATNYFEGKSSYQEEQFGFLQPSLSKNELQGNNWYGGKNQFY*