AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1062020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1062020 MtrunA17_Chr7g0240581 95.089 224 11 0 1 224 1 224 2.33e-159 439
Msa1062020 MtrunA17_Chr7g0240561 80.357 224 44 0 1 224 1 224 7.74e-136 380
Msa1062020 MtrunA17_Chr7g0240551 77.679 224 50 0 1 224 1 224 1.31e-130 367
Msa1062020 MtrunA17_Chr7g0240531 74.554 224 57 0 1 224 1 224 2.93e-124 351
Msa1062020 MtrunA17_Chr7g0240511 76.339 224 48 1 1 224 1 219 7.51e-124 349
Msa1062020 MtrunA17_Chr7g0240541 73.214 224 60 0 1 224 1 224 5.28e-122 345
Msa1062020 MtrunA17_Chr7g0240521 76.889 225 51 1 1 224 8 232 1.13e-116 332
Msa1062020 MtrunA17_Chr7g0240681 69.333 225 68 1 1 224 1 225 4.83e-115 327
Msa1062020 MtrunA17_Chr7g0240631 67.713 223 72 0 1 223 1 223 2.22e-114 326
Msa1062020 MtrunA17_Chr7g0240641 68.161 223 71 0 1 223 1 223 5.81e-114 325
Msa1062020 MtrunA17_Chr7g0240611 66.816 223 70 1 1 223 1 219 2.49e-107 308
Msa1062020 MtrunA17_Chr7g0240691 62.557 219 81 1 6 223 36 254 2.83e-103 299
Msa1062020 MtrunA17_Chr4g0008791 60.987 223 87 0 1 223 1 223 3.35e-103 297
Msa1062020 MtrunA17_Chr7g0240671 64.734 207 69 1 8 214 5 207 3.35e-96 280
Msa1062020 MtrunA17_Chr4g0008781 62.150 214 79 2 3 215 4 216 5.90e-94 274
Msa1062020 MtrunA17_Chr7g0240651 65.766 222 72 1 1 222 1 218 5.74e-93 271
Msa1062020 MtrunA17_Chr7g0240661 64.019 214 73 1 1 214 1 210 7.58e-88 259
Msa1062020 MtrunA17_Chr1g0196771 45.249 221 116 2 5 222 1 219 1.42e-65 202
Msa1062020 MtrunA17_Chr6g0483141 47.826 207 106 2 8 213 4 209 5.19e-64 198
Msa1062020 MtrunA17_Chr8g0379581 46.789 218 114 2 4 220 2 218 9.75e-64 197
Msa1062020 MtrunA17_Chr3g0132581 48.636 220 111 2 5 223 3 221 1.36e-62 194
Msa1062020 MtrunA17_Chr8g0379561 45.161 217 116 3 1 216 1 215 1.36e-58 184
Msa1062020 MtrunA17_Chr1g0212301 45.070 213 114 2 7 216 3 215 2.38e-58 183
Msa1062020 MtrunA17_Chr5g0417011 41.892 222 121 3 1 215 1 221 5.82e-58 182
Msa1062020 MtrunA17_Chr1g0196691 42.273 220 122 3 8 223 29 247 4.96e-56 178
Msa1062020 MtrunA17_Chr1g0196721 42.986 221 120 4 7 223 2 220 1.96e-54 173
Msa1062020 MtrunA17_Chr5g0415411 43.182 220 115 3 1 212 1 218 4.54e-54 173
Msa1062020 MtrunA17_Chr1g0196661 42.081 221 122 4 8 224 4 222 1.54e-53 171
Msa1062020 MtrunA17_Chr8g0363751 42.922 219 117 3 5 215 3 221 1.66e-51 166
Msa1062020 MtrunA17_Chr4g0029341 39.906 213 124 2 6 215 3 214 2.03e-51 166
Msa1062020 MtrunA17_Chr1g0196711 41.627 209 116 4 8 212 4 210 4.13e-50 162
Msa1062020 MtrunA17_Chr2g0312791 40.196 204 118 3 6 207 3 204 3.44e-44 147
Msa1062020 MtrunA17_Chr2g0312771 37.321 209 126 4 6 212 3 208 1.17e-42 143
Msa1062020 MtrunA17_Chr3g0107731 42.000 200 111 4 7 204 9 205 1.42e-42 143
Msa1062020 MtrunA17_Chr2g0312751 36.866 217 129 5 6 217 3 216 1.44e-42 143
Msa1062020 MtrunA17_Chr2g0312781 37.799 209 125 4 1 207 15 220 4.99e-42 142
Msa1062020 MtrunA17_Chr2g0312731 37.864 206 123 4 6 209 3 205 2.98e-41 140
Msa1062020 MtrunA17_Chr4g0069471 43.820 178 96 4 3 177 21 197 1.43e-39 135
Msa1062020 MtrunA17_Chr3g0107741 39.901 203 117 4 4 204 6 205 4.89e-39 134
Msa1062020 MtrunA17_Chr2g0312811 37.915 211 124 5 6 212 3 210 1.81e-38 132
Msa1062020 MtrunA17_Chr5g0433801 38.164 207 123 4 3 207 2 205 1.83e-38 132
Msa1062020 MtrunA17_Chr8g0357241 41.270 189 106 4 6 192 3 188 2.19e-38 132
Msa1062020 MtrunA17_Chr8g0391631 46.452 155 75 3 7 153 21 175 2.58e-38 130
Msa1062020 MtrunA17_Chr2g0312721 37.864 206 123 4 6 209 3 205 4.41e-38 131
Msa1062020 MtrunA17_Chr8g0357211 40.957 188 106 4 6 191 3 187 1.50e-37 130
Msa1062020 MtrunA17_Chr2g0312691 38.693 199 117 4 8 204 5 200 3.17e-36 127
Msa1062020 MtrunA17_Chr2g0312681 36.634 202 123 4 8 207 5 203 1.50e-35 125
Msa1062020 MtrunA17_Chr2g0312711 36.715 207 125 4 8 212 5 207 1.12e-30 112
Msa1062020 MtrunA17_Chr2g0313561 44.615 65 35 1 51 114 2 66 2.87e-15 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1062020 AT3G09270.1 50.893 224 106 4 3 223 2 224 1.55e-76 230
Msa1062020 AT2G29420.1 48.402 219 111 2 3 220 5 222 3.11e-72 219
Msa1062020 AT2G29490.1 47.847 209 106 3 1 207 1 208 4.04e-60 188
Msa1062020 AT2G29450.1 43.498 223 124 2 3 223 2 224 6.21e-57 180
Msa1062020 AT2G29460.1 43.694 222 122 3 1 220 1 221 1.71e-55 177
Msa1062020 AT2G29480.1 44.498 209 113 3 1 207 1 208 2.11e-54 174
Msa1062020 AT1G10370.1 43.439 221 116 3 1 214 1 219 4.57e-53 171
Msa1062020 AT2G29440.1 41.778 225 127 4 1 223 1 223 8.13e-53 170
Msa1062020 AT2G29470.1 43.810 210 114 4 1 207 1 209 1.16e-52 169
Msa1062020 AT1G69930.1 38.839 224 131 2 2 219 7 230 9.02e-52 167
Msa1062020 AT1G10360.1 41.262 206 114 2 6 204 4 209 2.92e-50 163
Msa1062020 AT1G27130.1 40.000 225 126 4 1 217 1 224 3.07e-49 160
Msa1062020 AT1G59700.1 44.037 218 114 3 5 214 4 221 4.92e-49 160
Msa1062020 AT1G59670.1 43.269 208 110 3 5 204 4 211 7.69e-49 160
Msa1062020 AT5G62480.1 39.367 221 123 4 5 215 6 225 2.10e-45 151
Msa1062020 AT1G27140.1 38.767 227 129 4 1 218 1 226 6.86e-45 150
Msa1062020 AT1G78320.1 40.865 208 118 4 5 210 2 206 3.34e-44 147
Msa1062020 AT1G17170.1 40.826 218 121 5 6 220 3 215 4.13e-44 147
Msa1062020 AT1G78370.1 40.488 205 117 4 10 212 7 208 1.69e-43 145
Msa1062020 AT1G78380.1 41.063 207 117 4 8 212 5 208 1.87e-43 145
Msa1062020 AT1G69920.1 37.445 227 132 4 1 217 26 252 1.38e-42 144
Msa1062020 AT1G78340.1 41.148 209 118 4 6 212 3 208 1.49e-42 143
Msa1062020 AT1G74590.1 34.361 227 142 3 1 221 1 226 6.25e-42 142
Msa1062020 AT1G53680.1 38.571 210 124 4 1 207 1 208 1.08e-40 139
Msa1062020 AT1G17180.1 37.799 209 125 4 6 212 3 208 1.93e-39 135
Msa1062020 AT1G78360.1 39.904 208 119 5 8 212 5 209 1.43e-38 133
Msa1062020 AT1G17190.1 38.785 214 124 5 1 212 1 209 2.23e-38 132
Msa1062020 AT3G43800.1 35.749 207 124 6 4 204 2 205 1.56e-35 125
Msa1062020 AT5G62480.2 31.364 220 116 4 5 215 6 199 7.39e-28 105
Msa1062020 AT1G78320.2 33.816 207 107 4 5 210 2 179 1.40e-27 104
Msa1062020 AT5G62480.3 31.364 220 115 4 5 215 6 198 1.62e-27 104
Msa1062020 AT2G02380.1 28.837 215 134 6 5 207 9 216 1.16e-16 76.3
Msa1062020 AT2G02390.1 29.358 218 133 7 3 207 4 213 3.27e-16 75.1
Msa1062020 AT2G02390.3 28.444 225 133 8 3 207 4 220 8.53e-14 68.6
Msa1062020 AT2G02390.2 40.206 97 54 2 3 96 4 99 2.72e-13 66.2

Find 58 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGCCTTGGCTCGTTTC+TGG 0.120947 7_2:+52514000 Msa1062020:CDS
TTACATACTTAGGGAATTTA+AGG 0.207184 7_2:+52515318 Msa1062020:three_prime_UTR
GAGCTTAAGGATAATTTCTT+TGG 0.282538 7_2:+52515049 Msa1062020:CDS
TAAGGATCAGAAGGCAATAT+TGG 0.299422 7_2:-52513975 None:intergenic
ACAGGAACCTTCTTGTAAAC+AGG 0.304362 7_2:-52513876 None:intergenic
AGTTATGGTGTTACATACTT+AGG 0.333531 7_2:+52515308 Msa1062020:three_prime_UTR
GCCTCCTAGAGAAGGACTTT+TGG 0.337294 7_2:+52515237 Msa1062020:CDS
GCTAATCAAACAACTGTCTT+AGG 0.337875 7_2:-52513694 None:intergenic
AATTCTTGAGAATGAGCTTA+AGG 0.354197 7_2:+52515036 Msa1062020:CDS
CGCTAAGATGGCATCAAATC+AGG 0.356601 7_2:+52513716 Msa1062020:exon
TTCTTTGGTGGAGAGGAGAT+TGG 0.368042 7_2:+52515064 Msa1062020:CDS
GGCAATTTATCCTTAACAAC+TGG 0.375316 7_2:-52515217 None:intergenic
TTGACTCCATTTGTAAAGCT+TGG 0.384299 7_2:-52515179 None:intergenic
CACCAACCATCCAGTTGTTA+AGG 0.388600 7_2:+52515207 Msa1062020:CDS
TGACTCCATTTGTAAAGCTT+GGG 0.390868 7_2:-52515178 None:intergenic
CTTCAAAATAGATTAAGTTA+TGG 0.394308 7_2:+52515293 Msa1062020:exon
GGAATTTAAGGAGAATTGTC+TGG 0.396718 7_2:+52515330 Msa1062020:three_prime_UTR
TTTGTTGCTTCTTGTACAAT+GGG 0.408906 7_2:-52515127 None:intergenic
TACAAATATGAGTTAGAAAA+GGG 0.419037 7_2:+52513819 Msa1062020:CDS
GTTTGTTGCTTCTTGTACAA+TGG 0.430175 7_2:-52515128 None:intergenic
ATCACAAGGGATTCTGATAT+AGG 0.433145 7_2:-52513918 None:intergenic
GACCAAGTTCATAGATGATA+AGG 0.434027 7_2:+52514022 Msa1062020:CDS
GTTATGGTGTTACATACTTA+GGG 0.434449 7_2:+52515309 Msa1062020:three_prime_UTR
ATCTCCACCCTGCTCACAAA+TGG 0.436953 7_2:-52513771 None:intergenic
ATACAAATATGAGTTAGAAA+AGG 0.446746 7_2:+52513818 Msa1062020:CDS
CTTAAGGATAATTTCTTTGG+TGG 0.454634 7_2:+52515052 Msa1062020:CDS
GGAGATTGCTCTAACATTGA+AGG 0.460355 7_2:+52513788 Msa1062020:CDS
TTGTTTGATTAGCGCTAAGA+TGG 0.460885 7_2:+52513704 Msa1062020:exon
TTTAAACAGTGAGAAATCCT+AGG 0.469805 7_2:-52515394 None:intergenic
AATTTCCCAAGCTTTACAAA+TGG 0.474830 7_2:+52515173 Msa1062020:CDS
TACCTTATCATCTATGAACT+TGG 0.479710 7_2:-52514024 None:intergenic
ATCCTTAACAACTGGATGGT+TGG 0.488666 7_2:-52515209 None:intergenic
AACTGTAAAAGAAACAATCC+TGG 0.514445 7_2:+52513625 None:intergenic
TTATCATTATGAACTAATAC+AGG 0.517988 7_2:-52513894 None:intergenic
GGATAATTTCTTTGGTGGAG+AGG 0.526031 7_2:+52515057 Msa1062020:CDS
GATAAATTGCCTCCTAGAGA+AGG 0.547220 7_2:+52515229 Msa1062020:CDS
TGATCCTTACAAAAGAGCCT+TGG 0.548089 7_2:+52513989 Msa1062020:CDS
TGGCAAGTCCATTTGTGAGC+AGG 0.549506 7_2:+52513763 Msa1062020:CDS
TTGAGTATGTCGATGAGACC+TGG 0.549849 7_2:+52513943 Msa1062020:CDS
TAAGCCAAAAGTCCTTCTCT+AGG 0.550201 7_2:-52515241 None:intergenic
CAGGAACCTTCTTGTAAACA+GGG 0.551890 7_2:-52513875 None:intergenic
ACTTCTATATATATAGAGCC+AGG 0.557159 7_2:-52513643 None:intergenic
AGAACGTGAGAAAGCTATTG+AGG 0.557970 7_2:+52514994 Msa1062020:CDS
ATTTATCCTTAACAACTGGA+TGG 0.561475 7_2:-52515213 None:intergenic
AATATGAGTTAGAAAAGGGA+GGG 0.564400 7_2:+52513823 Msa1062020:CDS
ATACAACCCTGTTTACAAGA+AGG 0.566628 7_2:+52513869 Msa1062020:CDS
GGCAAGTCCATTTGTGAGCA+GGG 0.574715 7_2:+52513764 Msa1062020:CDS
AAATATGAGTTAGAAAAGGG+AGG 0.578650 7_2:+52513822 Msa1062020:CDS
AAATTAGATATATGCAACCT+AGG 0.591911 7_2:+52515377 Msa1062020:three_prime_UTR
GAGATTGCTCTAACATTGAA+GGG 0.597295 7_2:+52513789 Msa1062020:CDS
CGACATACTCAAGAATCACA+AGG 0.607291 7_2:-52513932 None:intergenic
TAATTAAAATAGTGTAAACA+AGG 0.616902 7_2:-52515433 None:intergenic
GCCAAAAGTCCTTCTCTAGG+AGG 0.619286 7_2:-52515238 None:intergenic
ACCAGTGTTTAAGTGCTGCA+CGG 0.628510 7_2:+52514945 Msa1062020:intron
CTTGGTCCAGAAACGAGCCA+AGG 0.682377 7_2:-52514006 None:intergenic
TTGTTGCTTCTTGTACAATG+GGG 0.684989 7_2:-52515126 None:intergenic
GACATACTCAAGAATCACAA+GGG 0.702786 7_2:-52513931 None:intergenic
AAGTCCATTTGTGAGCAGGG+TGG 0.747804 7_2:+52513767 Msa1062020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTTCATTTTTAAATTA+CGG + chr7_2:52514448-52514467 Msa1062020:intron 10.0%
!!! TACTTTTCTTTTTAAAATTA+CGG - chr7_2:52514577-52514596 None:intergenic 10.0%
!!! TAGTTTTCATTTTTAAATTA+CGG + chr7_2:52514448-52514467 Msa1062020:intron 10.0%
!!! TACTTTTCTTTTTAAAATTA+CGG - chr7_2:52514577-52514596 None:intergenic 10.0%
!!! TCATTTTTAAAGTGTATAAA+TGG + chr7_2:52514122-52514141 Msa1062020:intron 15.0%
!!! CATTTTTAAAGTGTATAAAT+GGG + chr7_2:52514123-52514142 Msa1062020:intron 15.0%
!!! ATTTTTAAAGTGTATAAATG+GGG + chr7_2:52514124-52514143 Msa1062020:intron 15.0%
!! AATAAATATGATATTTGAAG+CGG - chr7_2:52514681-52514700 None:intergenic 15.0%
!! AATATTTAAGAAGAATAATC+TGG + chr7_2:52514811-52514830 Msa1062020:intron 15.0%
!! TAATTAAAATAGTGTAAACA+AGG - chr7_2:52515436-52515455 None:intergenic 15.0%
!!! TCATTTTTAAAGTGTATAAA+TGG + chr7_2:52514122-52514141 Msa1062020:intron 15.0%
!!! CATTTTTAAAGTGTATAAAT+GGG + chr7_2:52514123-52514142 Msa1062020:intron 15.0%
!!! ATTTTTAAAGTGTATAAATG+GGG + chr7_2:52514124-52514143 Msa1062020:intron 15.0%
!! AATAAATATGATATTTGAAG+CGG - chr7_2:52514681-52514700 None:intergenic 15.0%
!! AATATTTAAGAAGAATAATC+TGG + chr7_2:52514811-52514830 Msa1062020:intron 15.0%
!! TAATTAAAATAGTGTAAACA+AGG - chr7_2:52515436-52515455 None:intergenic 15.0%
!! ATACAAATATGAGTTAGAAA+AGG + chr7_2:52513818-52513837 Msa1062020:CDS 20.0%
!! TACAAATATGAGTTAGAAAA+GGG + chr7_2:52513819-52513838 Msa1062020:CDS 20.0%
!! TTATCATTATGAACTAATAC+AGG - chr7_2:52513897-52513916 None:intergenic 20.0%
!!! ACACTGGTTTTAAAAAATTA+AGG - chr7_2:52514933-52514952 None:intergenic 20.0%
!! CTTCAAAATAGATTAAGTTA+TGG + chr7_2:52515293-52515312 Msa1062020:exon 20.0%
!! ATACAAATATGAGTTAGAAA+AGG + chr7_2:52513818-52513837 Msa1062020:CDS 20.0%
!! TACAAATATGAGTTAGAAAA+GGG + chr7_2:52513819-52513838 Msa1062020:CDS 20.0%
!! TTATCATTATGAACTAATAC+AGG - chr7_2:52513897-52513916 None:intergenic 20.0%
!!! ACACTGGTTTTAAAAAATTA+AGG - chr7_2:52514933-52514952 None:intergenic 20.0%
!! CTTCAAAATAGATTAAGTTA+TGG + chr7_2:52515293-52515312 Msa1062020:exon 20.0%
! TGTATATTATATGCAGAGAT+CGG - chr7_2:52514167-52514186 None:intergenic 25.0%
! TAAAATATTTCACAAGCATG+TGG + chr7_2:52514257-52514276 Msa1062020:intron 25.0%
!!! ATGTATTGTTTTTCTCTATG+CGG - chr7_2:52514334-52514353 None:intergenic 25.0%
! ATAGAGAAAAACAATACATG+TGG + chr7_2:52514335-52514354 Msa1062020:intron 25.0%
! TTATTCAACAAAACGAGTAA+CGG + chr7_2:52514696-52514715 Msa1062020:intron 25.0%
! ATGACTATTATATATGCATG+TGG + chr7_2:52514719-52514738 Msa1062020:intron 25.0%
!! TGAAATATAATGACTTGTGA+CGG - chr7_2:52514768-52514787 None:intergenic 25.0%
! TTACATACTTAGGGAATTTA+AGG + chr7_2:52515318-52515337 Msa1062020:three_prime_UTR 25.0%
! AAATTAGATATATGCAACCT+AGG + chr7_2:52515377-52515396 Msa1062020:three_prime_UTR 25.0%
!!! ATGTTATTTTCATGGGAATA+AGG - chr7_2:52515470-52515489 None:intergenic 25.0%
! TGTATATTATATGCAGAGAT+CGG - chr7_2:52514167-52514186 None:intergenic 25.0%
! TAAAATATTTCACAAGCATG+TGG + chr7_2:52514257-52514276 Msa1062020:intron 25.0%
!!! ATGTATTGTTTTTCTCTATG+CGG - chr7_2:52514334-52514353 None:intergenic 25.0%
! ATAGAGAAAAACAATACATG+TGG + chr7_2:52514335-52514354 Msa1062020:intron 25.0%
! TTATTCAACAAAACGAGTAA+CGG + chr7_2:52514696-52514715 Msa1062020:intron 25.0%
! ATGACTATTATATATGCATG+TGG + chr7_2:52514719-52514738 Msa1062020:intron 25.0%
!! TGAAATATAATGACTTGTGA+CGG - chr7_2:52514768-52514787 None:intergenic 25.0%
! TTACATACTTAGGGAATTTA+AGG + chr7_2:52515318-52515337 Msa1062020:three_prime_UTR 25.0%
! AAATTAGATATATGCAACCT+AGG + chr7_2:52515377-52515396 Msa1062020:three_prime_UTR 25.0%
!!! ATGTTATTTTCATGGGAATA+AGG - chr7_2:52515470-52515489 None:intergenic 25.0%
ACTTCTATATATATAGAGCC+AGG - chr7_2:52513646-52513665 None:intergenic 30.0%
AAATATGAGTTAGAAAAGGG+AGG + chr7_2:52513822-52513841 Msa1062020:CDS 30.0%
AATATGAGTTAGAAAAGGGA+GGG + chr7_2:52513823-52513842 Msa1062020:CDS 30.0%
!! CAATATTGGATTTTGTTTCC+AGG - chr7_2:52513964-52513983 None:intergenic 30.0%
TACCTTATCATCTATGAACT+TGG - chr7_2:52514027-52514046 None:intergenic 30.0%
AAATACCACAAACTTCACTA+AGG + chr7_2:52514380-52514399 Msa1062020:intron 30.0%
AATACCACAAACTTCACTAA+GGG + chr7_2:52514381-52514400 Msa1062020:intron 30.0%
AATTCTTGAGAATGAGCTTA+AGG + chr7_2:52515036-52515055 Msa1062020:CDS 30.0%
GAGCTTAAGGATAATTTCTT+TGG + chr7_2:52515049-52515068 Msa1062020:CDS 30.0%
CTTAAGGATAATTTCTTTGG+TGG + chr7_2:52515052-52515071 Msa1062020:CDS 30.0%
! TTTGTTGCTTCTTGTACAAT+GGG - chr7_2:52515130-52515149 None:intergenic 30.0%
AATTTCCCAAGCTTTACAAA+TGG + chr7_2:52515173-52515192 Msa1062020:CDS 30.0%
ATTTATCCTTAACAACTGGA+TGG - chr7_2:52515216-52515235 None:intergenic 30.0%
!! AGTTATGGTGTTACATACTT+AGG + chr7_2:52515308-52515327 Msa1062020:three_prime_UTR 30.0%
!! GTTATGGTGTTACATACTTA+GGG + chr7_2:52515309-52515328 Msa1062020:three_prime_UTR 30.0%
TTTAAACAGTGAGAAATCCT+AGG - chr7_2:52515397-52515416 None:intergenic 30.0%
TCTCACTGTTTAAAAAGTCT+AGG + chr7_2:52515403-52515422 Msa1062020:three_prime_UTR 30.0%
!!! TGATGGCATGTTATTTTCAT+GGG - chr7_2:52515477-52515496 None:intergenic 30.0%
ACTTCTATATATATAGAGCC+AGG - chr7_2:52513646-52513665 None:intergenic 30.0%
AAATATGAGTTAGAAAAGGG+AGG + chr7_2:52513822-52513841 Msa1062020:CDS 30.0%
AATATGAGTTAGAAAAGGGA+GGG + chr7_2:52513823-52513842 Msa1062020:CDS 30.0%
!! CAATATTGGATTTTGTTTCC+AGG - chr7_2:52513964-52513983 None:intergenic 30.0%
TACCTTATCATCTATGAACT+TGG - chr7_2:52514027-52514046 None:intergenic 30.0%
AAATACCACAAACTTCACTA+AGG + chr7_2:52514380-52514399 Msa1062020:intron 30.0%
AATACCACAAACTTCACTAA+GGG + chr7_2:52514381-52514400 Msa1062020:intron 30.0%
AATTCTTGAGAATGAGCTTA+AGG + chr7_2:52515036-52515055 Msa1062020:CDS 30.0%
GAGCTTAAGGATAATTTCTT+TGG + chr7_2:52515049-52515068 Msa1062020:CDS 30.0%
CTTAAGGATAATTTCTTTGG+TGG + chr7_2:52515052-52515071 Msa1062020:CDS 30.0%
! TTTGTTGCTTCTTGTACAAT+GGG - chr7_2:52515130-52515149 None:intergenic 30.0%
AATTTCCCAAGCTTTACAAA+TGG + chr7_2:52515173-52515192 Msa1062020:CDS 30.0%
ATTTATCCTTAACAACTGGA+TGG - chr7_2:52515216-52515235 None:intergenic 30.0%
!! AGTTATGGTGTTACATACTT+AGG + chr7_2:52515308-52515327 Msa1062020:three_prime_UTR 30.0%
!! GTTATGGTGTTACATACTTA+GGG + chr7_2:52515309-52515328 Msa1062020:three_prime_UTR 30.0%
TTTAAACAGTGAGAAATCCT+AGG - chr7_2:52515397-52515416 None:intergenic 30.0%
TCTCACTGTTTAAAAAGTCT+AGG + chr7_2:52515403-52515422 Msa1062020:three_prime_UTR 30.0%
!!! TGATGGCATGTTATTTTCAT+GGG - chr7_2:52515477-52515496 None:intergenic 30.0%
GCTAATCAAACAACTGTCTT+AGG - chr7_2:52513697-52513716 None:intergenic 35.0%
TTGTTTGATTAGCGCTAAGA+TGG + chr7_2:52513704-52513723 Msa1062020:exon 35.0%
! CAGGAAAATGTGAAGCTTTT+TGG + chr7_2:52513735-52513754 Msa1062020:CDS 35.0%
!!! TGTGAAGCTTTTTGGAATGA+TGG + chr7_2:52513743-52513762 Msa1062020:CDS 35.0%
GAGATTGCTCTAACATTGAA+GGG + chr7_2:52513789-52513808 Msa1062020:CDS 35.0%
ATACAACCCTGTTTACAAGA+AGG + chr7_2:52513869-52513888 Msa1062020:CDS 35.0%
ATCACAAGGGATTCTGATAT+AGG - chr7_2:52513921-52513940 None:intergenic 35.0%
GACATACTCAAGAATCACAA+GGG - chr7_2:52513934-52513953 None:intergenic 35.0%
TAAGGATCAGAAGGCAATAT+TGG - chr7_2:52513978-52513997 None:intergenic 35.0%
GACCAAGTTCATAGATGATA+AGG + chr7_2:52514022-52514041 Msa1062020:CDS 35.0%
GAATTACGTTCATGTGATCT+CGG - chr7_2:52514208-52514227 None:intergenic 35.0%
ATATTTCACAAGCATGTGGA+TGG + chr7_2:52514261-52514280 Msa1062020:intron 35.0%
ATACGCTAGAGAGACATTTA+GGG - chr7_2:52514644-52514663 None:intergenic 35.0%
TATACGCTAGAGAGACATTT+AGG - chr7_2:52514645-52514664 None:intergenic 35.0%
!! TATAATGACTTGTGACGGAT+TGG - chr7_2:52514763-52514782 None:intergenic 35.0%
! TTGTTGCTTCTTGTACAATG+GGG - chr7_2:52515129-52515148 None:intergenic 35.0%
! GTTTGTTGCTTCTTGTACAA+TGG - chr7_2:52515131-52515150 None:intergenic 35.0%
TGACTCCATTTGTAAAGCTT+GGG - chr7_2:52515181-52515200 None:intergenic 35.0%
TTGACTCCATTTGTAAAGCT+TGG - chr7_2:52515182-52515201 None:intergenic 35.0%
GGCAATTTATCCTTAACAAC+TGG - chr7_2:52515220-52515239 None:intergenic 35.0%
GGAATTTAAGGAGAATTGTC+TGG + chr7_2:52515330-52515349 Msa1062020:three_prime_UTR 35.0%
!!! GTGATGGCATGTTATTTTCA+TGG - chr7_2:52515478-52515497 None:intergenic 35.0%
GCTAATCAAACAACTGTCTT+AGG - chr7_2:52513697-52513716 None:intergenic 35.0%
TTGTTTGATTAGCGCTAAGA+TGG + chr7_2:52513704-52513723 Msa1062020:exon 35.0%
! CAGGAAAATGTGAAGCTTTT+TGG + chr7_2:52513735-52513754 Msa1062020:CDS 35.0%
!!! TGTGAAGCTTTTTGGAATGA+TGG + chr7_2:52513743-52513762 Msa1062020:CDS 35.0%
GAGATTGCTCTAACATTGAA+GGG + chr7_2:52513789-52513808 Msa1062020:CDS 35.0%
ATACAACCCTGTTTACAAGA+AGG + chr7_2:52513869-52513888 Msa1062020:CDS 35.0%
ATCACAAGGGATTCTGATAT+AGG - chr7_2:52513921-52513940 None:intergenic 35.0%
GACATACTCAAGAATCACAA+GGG - chr7_2:52513934-52513953 None:intergenic 35.0%
TAAGGATCAGAAGGCAATAT+TGG - chr7_2:52513978-52513997 None:intergenic 35.0%
GACCAAGTTCATAGATGATA+AGG + chr7_2:52514022-52514041 Msa1062020:CDS 35.0%
GAATTACGTTCATGTGATCT+CGG - chr7_2:52514208-52514227 None:intergenic 35.0%
ATATTTCACAAGCATGTGGA+TGG + chr7_2:52514261-52514280 Msa1062020:intron 35.0%
ATACGCTAGAGAGACATTTA+GGG - chr7_2:52514644-52514663 None:intergenic 35.0%
TATACGCTAGAGAGACATTT+AGG - chr7_2:52514645-52514664 None:intergenic 35.0%
!! TATAATGACTTGTGACGGAT+TGG - chr7_2:52514763-52514782 None:intergenic 35.0%
! TTGTTGCTTCTTGTACAATG+GGG - chr7_2:52515129-52515148 None:intergenic 35.0%
! GTTTGTTGCTTCTTGTACAA+TGG - chr7_2:52515131-52515150 None:intergenic 35.0%
TGACTCCATTTGTAAAGCTT+GGG - chr7_2:52515181-52515200 None:intergenic 35.0%
TTGACTCCATTTGTAAAGCT+TGG - chr7_2:52515182-52515201 None:intergenic 35.0%
GGCAATTTATCCTTAACAAC+TGG - chr7_2:52515220-52515239 None:intergenic 35.0%
GGAATTTAAGGAGAATTGTC+TGG + chr7_2:52515330-52515349 Msa1062020:three_prime_UTR 35.0%
!!! GTGATGGCATGTTATTTTCA+TGG - chr7_2:52515478-52515497 None:intergenic 35.0%
GGAGATTGCTCTAACATTGA+AGG + chr7_2:52513788-52513807 Msa1062020:CDS 40.0%
CAGGAACCTTCTTGTAAACA+GGG - chr7_2:52513878-52513897 None:intergenic 40.0%
ACAGGAACCTTCTTGTAAAC+AGG - chr7_2:52513879-52513898 None:intergenic 40.0%
CGACATACTCAAGAATCACA+AGG - chr7_2:52513935-52513954 None:intergenic 40.0%
! GCTCTTTTGTAAGGATCAGA+AGG - chr7_2:52513987-52514006 None:intergenic 40.0%
TGATCCTTACAAAAGAGCCT+TGG + chr7_2:52513989-52514008 Msa1062020:CDS 40.0%
! TCTTTATTTGTGCGCATGTG+TGG + chr7_2:52514233-52514252 Msa1062020:intron 40.0%
! AACTTCACTAAGGGTGTGTT+TGG + chr7_2:52514390-52514409 Msa1062020:intron 40.0%
TGTGTTTGGATGGAAGGTTT+AGG + chr7_2:52514404-52514423 Msa1062020:intron 40.0%
! ATTTGAAGCGGTTCTCAATG+TGG - chr7_2:52514669-52514688 None:intergenic 40.0%
AGAACGTGAGAAAGCTATTG+AGG + chr7_2:52514994-52515013 Msa1062020:CDS 40.0%
GGATAATTTCTTTGGTGGAG+AGG + chr7_2:52515057-52515076 Msa1062020:CDS 40.0%
! CTGCAGTCTTCATAGCTTTT+TGG + chr7_2:52515101-52515120 Msa1062020:CDS 40.0%
ATCCTTAACAACTGGATGGT+TGG - chr7_2:52515212-52515231 None:intergenic 40.0%
GATAAATTGCCTCCTAGAGA+AGG + chr7_2:52515229-52515248 Msa1062020:CDS 40.0%
TAAGCCAAAAGTCCTTCTCT+AGG - chr7_2:52515244-52515263 None:intergenic 40.0%
GGAGATTGCTCTAACATTGA+AGG + chr7_2:52513788-52513807 Msa1062020:CDS 40.0%
CAGGAACCTTCTTGTAAACA+GGG - chr7_2:52513878-52513897 None:intergenic 40.0%
ACAGGAACCTTCTTGTAAAC+AGG - chr7_2:52513879-52513898 None:intergenic 40.0%
CGACATACTCAAGAATCACA+AGG - chr7_2:52513935-52513954 None:intergenic 40.0%
! GCTCTTTTGTAAGGATCAGA+AGG - chr7_2:52513987-52514006 None:intergenic 40.0%
TGATCCTTACAAAAGAGCCT+TGG + chr7_2:52513989-52514008 Msa1062020:CDS 40.0%
! TCTTTATTTGTGCGCATGTG+TGG + chr7_2:52514233-52514252 Msa1062020:intron 40.0%
! AACTTCACTAAGGGTGTGTT+TGG + chr7_2:52514390-52514409 Msa1062020:intron 40.0%
TGTGTTTGGATGGAAGGTTT+AGG + chr7_2:52514404-52514423 Msa1062020:intron 40.0%
! ATTTGAAGCGGTTCTCAATG+TGG - chr7_2:52514669-52514688 None:intergenic 40.0%
AGAACGTGAGAAAGCTATTG+AGG + chr7_2:52514994-52515013 Msa1062020:CDS 40.0%
GGATAATTTCTTTGGTGGAG+AGG + chr7_2:52515057-52515076 Msa1062020:CDS 40.0%
! CTGCAGTCTTCATAGCTTTT+TGG + chr7_2:52515101-52515120 Msa1062020:CDS 40.0%
ATCCTTAACAACTGGATGGT+TGG - chr7_2:52515212-52515231 None:intergenic 40.0%
GATAAATTGCCTCCTAGAGA+AGG + chr7_2:52515229-52515248 Msa1062020:CDS 40.0%
TAAGCCAAAAGTCCTTCTCT+AGG - chr7_2:52515244-52515263 None:intergenic 40.0%
CGCTAAGATGGCATCAAATC+AGG + chr7_2:52513716-52513735 Msa1062020:exon 45.0%
TTGAGTATGTCGATGAGACC+TGG + chr7_2:52513943-52513962 Msa1062020:CDS 45.0%
AGAGATCGGAGTTCGAACTT+CGG - chr7_2:52514153-52514172 None:intergenic 45.0%
! TGATCTCGGTTCAGTTGGTA+GGG - chr7_2:52514194-52514213 None:intergenic 45.0%
TCATGTGATCTCGGTTCAGT+TGG - chr7_2:52514199-52514218 None:intergenic 45.0%
CACACCCTTAGTGAAGTTTG+TGG - chr7_2:52514388-52514407 None:intergenic 45.0%
! TCACTAAGGGTGTGTTTGGA+TGG + chr7_2:52514394-52514413 Msa1062020:intron 45.0%
! TAAGGGTGTGTTTGGATGGA+AGG + chr7_2:52514398-52514417 Msa1062020:intron 45.0%
TTGGATGGAAGGTTTAGGAG+TGG + chr7_2:52514409-52514428 Msa1062020:intron 45.0%
ATGGAAGGTTTAGGAGTGGA+AGG + chr7_2:52514413-52514432 Msa1062020:intron 45.0%
TGGAAGGTTTAGGAGTGGAA+GGG + chr7_2:52514414-52514433 Msa1062020:intron 45.0%
TTGGATTGAGGGTTTAGGAG+GGG - chr7_2:52514616-52514635 None:intergenic 45.0%
CTTGGATTGAGGGTTTAGGA+GGG - chr7_2:52514617-52514636 None:intergenic 45.0%
TGTGCTTGGATTGAGGGTTT+AGG - chr7_2:52514621-52514640 None:intergenic 45.0%
TTAGGGTGTGCTTGGATTGA+GGG - chr7_2:52514627-52514646 None:intergenic 45.0%
! TTTAGGGTGTGCTTGGATTG+AGG - chr7_2:52514628-52514647 None:intergenic 45.0%
! AGAGACATTTAGGGTGTGCT+TGG - chr7_2:52514635-52514654 None:intergenic 45.0%
! ACCAGTGTTTAAGTGCTGCA+CGG + chr7_2:52514945-52514964 Msa1062020:intron 45.0%
TTCTTTGGTGGAGAGGAGAT+TGG + chr7_2:52515064-52515083 Msa1062020:CDS 45.0%
CACCAACCATCCAGTTGTTA+AGG + chr7_2:52515207-52515226 Msa1062020:CDS 45.0%
CGCTAAGATGGCATCAAATC+AGG + chr7_2:52513716-52513735 Msa1062020:exon 45.0%
TTGAGTATGTCGATGAGACC+TGG + chr7_2:52513943-52513962 Msa1062020:CDS 45.0%
AGAGATCGGAGTTCGAACTT+CGG - chr7_2:52514153-52514172 None:intergenic 45.0%
! TGATCTCGGTTCAGTTGGTA+GGG - chr7_2:52514194-52514213 None:intergenic 45.0%
TCATGTGATCTCGGTTCAGT+TGG - chr7_2:52514199-52514218 None:intergenic 45.0%
CACACCCTTAGTGAAGTTTG+TGG - chr7_2:52514388-52514407 None:intergenic 45.0%
! TCACTAAGGGTGTGTTTGGA+TGG + chr7_2:52514394-52514413 Msa1062020:intron 45.0%
! TAAGGGTGTGTTTGGATGGA+AGG + chr7_2:52514398-52514417 Msa1062020:intron 45.0%
TTGGATGGAAGGTTTAGGAG+TGG + chr7_2:52514409-52514428 Msa1062020:intron 45.0%
ATGGAAGGTTTAGGAGTGGA+AGG + chr7_2:52514413-52514432 Msa1062020:intron 45.0%
TGGAAGGTTTAGGAGTGGAA+GGG + chr7_2:52514414-52514433 Msa1062020:intron 45.0%
TTGGATTGAGGGTTTAGGAG+GGG - chr7_2:52514616-52514635 None:intergenic 45.0%
CTTGGATTGAGGGTTTAGGA+GGG - chr7_2:52514617-52514636 None:intergenic 45.0%
TGTGCTTGGATTGAGGGTTT+AGG - chr7_2:52514621-52514640 None:intergenic 45.0%
TTAGGGTGTGCTTGGATTGA+GGG - chr7_2:52514627-52514646 None:intergenic 45.0%
! TTTAGGGTGTGCTTGGATTG+AGG - chr7_2:52514628-52514647 None:intergenic 45.0%
! AGAGACATTTAGGGTGTGCT+TGG - chr7_2:52514635-52514654 None:intergenic 45.0%
! ACCAGTGTTTAAGTGCTGCA+CGG + chr7_2:52514945-52514964 Msa1062020:intron 45.0%
TTCTTTGGTGGAGAGGAGAT+TGG + chr7_2:52515064-52515083 Msa1062020:CDS 45.0%
CACCAACCATCCAGTTGTTA+AGG + chr7_2:52515207-52515226 Msa1062020:CDS 45.0%
TGGCAAGTCCATTTGTGAGC+AGG + chr7_2:52513763-52513782 Msa1062020:CDS 50.0%
GGCAAGTCCATTTGTGAGCA+GGG + chr7_2:52513764-52513783 Msa1062020:CDS 50.0%
AAGTCCATTTGTGAGCAGGG+TGG + chr7_2:52513767-52513786 Msa1062020:CDS 50.0%
ATCTCCACCCTGCTCACAAA+TGG - chr7_2:52513774-52513793 None:intergenic 50.0%
! CGAGCCAAGGCTCTTTTGTA+AGG - chr7_2:52513996-52514015 None:intergenic 50.0%
! AAAGAGCCTTGGCTCGTTTC+TGG + chr7_2:52514000-52514019 Msa1062020:CDS 50.0%
GTGATCTCGGTTCAGTTGGT+AGG - chr7_2:52514195-52514214 None:intergenic 50.0%
TTAGGAGTGGAAGGGAGATG+AGG + chr7_2:52514422-52514441 Msa1062020:intron 50.0%
TAGGAGTGGAAGGGAGATGA+GGG + chr7_2:52514423-52514442 Msa1062020:intron 50.0%
GCTTGGATTGAGGGTTTAGG+AGG - chr7_2:52514618-52514637 None:intergenic 50.0%
TCCGTGCAGCACTTAAACAC+TGG - chr7_2:52514949-52514968 None:intergenic 50.0%
! GCCTCCTAGAGAAGGACTTT+TGG + chr7_2:52515237-52515256 Msa1062020:CDS 50.0%
GCCAAAAGTCCTTCTCTAGG+AGG - chr7_2:52515241-52515260 None:intergenic 50.0%
TGGCAAGTCCATTTGTGAGC+AGG + chr7_2:52513763-52513782 Msa1062020:CDS 50.0%
GGCAAGTCCATTTGTGAGCA+GGG + chr7_2:52513764-52513783 Msa1062020:CDS 50.0%
AAGTCCATTTGTGAGCAGGG+TGG + chr7_2:52513767-52513786 Msa1062020:CDS 50.0%
ATCTCCACCCTGCTCACAAA+TGG - chr7_2:52513774-52513793 None:intergenic 50.0%
! CGAGCCAAGGCTCTTTTGTA+AGG - chr7_2:52513996-52514015 None:intergenic 50.0%
! AAAGAGCCTTGGCTCGTTTC+TGG + chr7_2:52514000-52514019 Msa1062020:CDS 50.0%
GTGATCTCGGTTCAGTTGGT+AGG - chr7_2:52514195-52514214 None:intergenic 50.0%
TTAGGAGTGGAAGGGAGATG+AGG + chr7_2:52514422-52514441 Msa1062020:intron 50.0%
TAGGAGTGGAAGGGAGATGA+GGG + chr7_2:52514423-52514442 Msa1062020:intron 50.0%
GCTTGGATTGAGGGTTTAGG+AGG - chr7_2:52514618-52514637 None:intergenic 50.0%
TCCGTGCAGCACTTAAACAC+TGG - chr7_2:52514949-52514968 None:intergenic 50.0%
! GCCTCCTAGAGAAGGACTTT+TGG + chr7_2:52515237-52515256 Msa1062020:CDS 50.0%
GCCAAAAGTCCTTCTCTAGG+AGG - chr7_2:52515241-52515260 None:intergenic 50.0%
CTTGGTCCAGAAACGAGCCA+AGG - chr7_2:52514009-52514028 None:intergenic 55.0%
TTGAGGGTTTAGGAGGGGCA+GGG - chr7_2:52514611-52514630 None:intergenic 55.0%
ATTGAGGGTTTAGGAGGGGC+AGG - chr7_2:52514612-52514631 None:intergenic 55.0%
CTTGGTCCAGAAACGAGCCA+AGG - chr7_2:52514009-52514028 None:intergenic 55.0%
TTGAGGGTTTAGGAGGGGCA+GGG - chr7_2:52514611-52514630 None:intergenic 55.0%
ATTGAGGGTTTAGGAGGGGC+AGG - chr7_2:52514612-52514631 None:intergenic 55.0%
TAGGAGGGGCAGGGAGAGGA+GGG - chr7_2:52514602-52514621 None:intergenic 65.0%
TTAGGAGGGGCAGGGAGAGG+AGG - chr7_2:52514603-52514622 None:intergenic 65.0%
GGTTTAGGAGGGGCAGGGAG+AGG - chr7_2:52514606-52514625 None:intergenic 65.0%
TAGGAGGGGCAGGGAGAGGA+GGG - chr7_2:52514602-52514621 None:intergenic 65.0%
TTAGGAGGGGCAGGGAGAGG+AGG - chr7_2:52514603-52514622 None:intergenic 65.0%
GGTTTAGGAGGGGCAGGGAG+AGG - chr7_2:52514606-52514625 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 52513631 52515497 52513631 ID=Msa1062020;Name=Msa1062020
chr7_2 mRNA 52513631 52515497 52513631 ID=Msa1062020-mRNA-1;Parent=Msa1062020;Name=Msa1062020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=92|1|1|1|1|1|2|194|224
chr7_2 exon 52513631 52514043 52513631 ID=Msa1062020-mRNA-1:exon:9486;Parent=Msa1062020-mRNA-1
chr7_2 exon 52514950 52515497 52514950 ID=Msa1062020-mRNA-1:exon:9487;Parent=Msa1062020-mRNA-1
chr7_2 five_prime_UTR 52513631 52513722 52513631 ID=Msa1062020-mRNA-1:five_prime_utr;Parent=Msa1062020-mRNA-1
chr7_2 CDS 52513723 52514043 52513723 ID=Msa1062020-mRNA-1:cds;Parent=Msa1062020-mRNA-1
chr7_2 CDS 52514950 52515303 52514950 ID=Msa1062020-mRNA-1:cds;Parent=Msa1062020-mRNA-1
chr7_2 three_prime_UTR 52515304 52515497 52515304 ID=Msa1062020-mRNA-1:three_prime_utr;Parent=Msa1062020-mRNA-1
Gene Sequence

>Msa1062020

ATGGCATCAAATCAGGAAAATGTGAAGCTTTTTGGAATGATGGCAAGTCCATTTGTGAGCAGGGTGGAGATTGCTCTAACATTGAAGGGAGTTGAATACAAATATGAGTTAGAAAAGGGAGGGAACTTGAGTGATACACTCATAAAATACAACCCTGTTTACAAGAAGGTTCCTGTATTAGTTCATAATGATAAGCCTATATCAGAATCCCTTGTGATTCTTGAGTATGTCGATGAGACCTGGAAACAAAATCCAATATTGCCTTCTGATCCTTACAAAAGAGCCTTGGCTCGTTTCTGGACCAAGTTCATAGATGATAAGTGTTTAAGTGCTGCACGGAAAGCTGCTTTCACTATTGATGAGCAAGAACGTGAGAAAGCTATTGAGGAAACAGAAGAGACTTTTCAAATTCTTGAGAATGAGCTTAAGGATAATTTCTTTGGTGGAGAGGAGATTGGAATTGTTGATATTGCTGCAGTCTTCATAGCTTTTTGGTTCCCCATTGTACAAGAAGCAACAAACTTGAAGTTGTTCACTAGTGAGAAATTTCCCAAGCTTTACAAATGGAGTCAAGACTTCACCAACCATCCAGTTGTTAAGGATAAATTGCCTCCTAGAGAAGGACTTTTGGCTTACTTTAAAGCTCGTTATGAAAGTCTTCTTGCTTCAAAATAG
Protein sequence

>Msa1062020

MASNQENVKLFGMMASPFVSRVEIALTLKGVEYKYELEKGGNLSDTLIKYNPVYKKVPVLVHNDKPISESLVILEYVDETWKQNPILPSDPYKRALARFWTKFIDDKCLSAARKAAFTIDEQEREKAIEETEETFQILENELKDNFFGGEEIGIVDIAAVFIAFWFPIVQEATNLKLFTSEKFPKLYKWSQDFTNHPVVKDKLPPREGLLAYFKARYESLLASK*