AlfalfaGEDB | Msa1023310

M. sativa cultivar zhongmu-4 / Msa1023310

KEGG: K00799
GO: -
PFAM: PF13417.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023310	XP_003623195.1	60.452	177	34	1	5	145	1	177	4.98e-60	202
Msa1023310	XP_003623195.1	94.872	195	10	0	148	342	30	224	4.23e-132	386

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023310	sp\|P32110\|GSTX6_SOYBN	71.981	207	54	2	137	342	22	225	2.41e-98	293
Msa1023310	sp\|P32110\|GSTX6_SOYBN	50.777	193	54	4	5	156	1	193	7.14e-50	169

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023310	G7L6G9	60.452	177	34	1	5	145	1	177	2.38e-60	202
Msa1023310	G7L6G9	94.872	195	10	0	148	342	30	224	2.02e-132	386

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001830	Msa1023310	0.877851	4.511303e-69	-8.615850e-47
Msa0002250	Msa1023310	0.817867	2.639709e-52	-8.615850e-47
Msa0007470	Msa1023310	0.841124	5.746314e-58	-8.615850e-47
Msa0015090	Msa1023310	0.833682	4.634069e-56	-8.615850e-47
Msa0017320	Msa1023310	0.806047	9.944563e-50	-8.615850e-47
Msa0026630	Msa1023310	0.849496	3.114827e-60	-8.615850e-47
Msa0032080	Msa1023310	0.820099	8.203611e-53	-8.615850e-47
Msa0035080	Msa1023310	0.816596	5.097819e-52	-8.615850e-47
Msa0041840	Msa1023310	0.831187	1.923907e-55	-8.615850e-47
Msa0049750	Msa1023310	0.824647	7.205219e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023310	MtrunA17_Chr7g0240561	94.872	195	10	0	148	342	30	224	3.89e-136	386
Msa1023310	MtrunA17_Chr7g0240561	60.452	177	34	1	5	145	1	177	4.58e-64	202
Msa1023310	MtrunA17_Chr7g0240581	81.538	195	36	0	148	342	30	224	1.11e-115	334
Msa1023310	MtrunA17_Chr7g0240581	74.286	175	9	1	5	143	1	175	5.54e-80	243
Msa1023310	MtrunA17_Chr7g0240551	79.487	195	40	0	148	342	30	224	2.80e-110	320
Msa1023310	MtrunA17_Chr7g0240551	60.109	183	37	1	5	151	1	183	1.10e-63	201
Msa1023310	MtrunA17_Chr7g0240511	78.462	195	37	1	148	342	30	219	1.05e-105	308
Msa1023310	MtrunA17_Chr7g0240511	57.627	177	34	2	5	145	1	172	3.02e-55	179
Msa1023310	MtrunA17_Chr7g0240531	77.436	195	44	0	148	342	30	224	1.74e-105	308
Msa1023310	MtrunA17_Chr7g0240531	58.192	177	38	1	5	145	1	177	6.37e-59	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023310	AT3G09270.1	50.254	197	94	4	148	341	29	224	9.26e-61	194
Msa1023310	AT3G09270.1	39.796	196	76	5	7	160	2	197	1.21e-31	119
Msa1023310	AT2G29420.1	46.875	192	100	2	148	338	32	222	1.27e-59	191
Msa1023310	AT2G29420.1	40.805	174	67	3	7	144	5	178	2.94e-30	115
Msa1023310	AT2G29450.1	46.597	191	97	3	137	325	21	208	4.93e-46	156
Msa1023310	AT2G29450.1	38.415	164	63	4	7	132	2	165	1.18e-18	84.0
Msa1023310	AT2G29490.1	42.500	200	111	4	129	325	10	208	7.16e-46	156
Msa1023310	AT2G29490.1	43.030	165	57	4	5	132	1	165	1.27e-23	97.4
Msa1023310	AT2G29460.1	41.667	216	122	4	129	341	10	224	3.16e-45	154
Msa1023310	AT2G29460.1	39.623	159	60	3	5	127	1	159	1.84e-19	86.3

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Find 72 sgRNAs with CRISPR-Local

Find 817 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTGCCTCGTAGAGAA+GGG	0.551755	7_1:+50679956	Msa1023310:CDS
AAAGAGCCTTGGCTCGTTTC+TGG	0.160272	7_1:+50679095	Msa1023310:CDS
AAATATGAGGAAGAAAAGCA+GGG	0.615123	7_1:+50678917	Msa1023310:CDS
AAATATGAGTTAGAAAAGGG+AGG	0.578650	7_1:+50673417	Msa1023310:CDS
AAATTAGATATATTGAGCCT+TGG	0.491800	7_1:+50680115	Msa1023310:three_prime_UTR
AAGTCCATTTGTGAGCAGGG+TGG	0.747804	7_1:+50673362	Msa1023310:CDS
AATATGAGGAAGAAAAGCAG+GGG	0.696106	7_1:+50678918	Msa1023310:CDS
AATATGAGTTAGAAAAGGGA+GGG	0.564400	7_1:+50673418	Msa1023310:CDS
AATTAGCATAGAAGTATATT+CGG	0.378495	7_1:-50680237	None:intergenic
AATTTCCTAAGCTTTACAAT+TGG	0.445365	7_1:+50679899	Msa1023310:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTAAACAGTGAGAAATCCT+AGG	-	chr7_1:50667326-50667345	None:intergenic	30.0%
TCTCACTGTTTAAAAAGTCT+AGG	+	chr7_1:50667332-50667351	Msa1023310:intron	30.0%
AAGTGCGTTAAAACTGATAT+TGG	+	chr7_1:50667621-50667640	Msa1023310:intron	30.0%
AACCTGAACAAATAATGAGA+GGG	+	chr7_1:50667879-50667898	Msa1023310:intron	30.0%
GAAAAACCTAATATCATCGT+CGG	+	chr7_1:50668206-50668225	Msa1023310:intron	30.0%
CGTCTTACTTATACTTTCTT+AGG	+	chr7_1:50668516-50668535	Msa1023310:intron	30.0%
CGAGAATAGAACATCATTAT+AGG	-	chr7_1:50668585-50668604	None:intergenic	30.0%
ACTAAGAAATCTCAAACCAA+AGG	+	chr7_1:50669152-50669171	Msa1023310:intron	30.0%
GTAGAAGCTTAGAAATACTT+GGG	-	chr7_1:50669223-50669242	None:intergenic	30.0%
ATGTAGAGATCAAATGAAGA+AGG	+	chr7_1:50669256-50669275	Msa1023310:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	50667241	50680314	50667241	ID=Msa1023310;Name=Msa1023310
chr7_1	mRNA	50667241	50680314	50667241	ID=Msa1023310-mRNA-1;Parent=Msa1023310;Name=Msa1023310-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0\|0.25\|0.2\|1\|0.25\|0.2\|5\|285\|342
chr7_1	exon	50667241	50667245	50667241	ID=Msa1023310-mRNA-1:exon:9935;Parent=Msa1023310-mRNA-1
chr7_1	exon	50673311	50673530	50673311	ID=Msa1023310-mRNA-1:exon:9936;Parent=Msa1023310-mRNA-1
chr7_1	exon	50674671	50674886	50674671	ID=Msa1023310-mRNA-1:exon:9937;Parent=Msa1023310-mRNA-1
chr7_1	exon	50678905	50679138	50678905	ID=Msa1023310-mRNA-1:exon:9938;Parent=Msa1023310-mRNA-1
chr7_1	exon	50679676	50680314	50679676	ID=Msa1023310-mRNA-1:exon:9939;Parent=Msa1023310-mRNA-1
chr7_1	CDS	50667241	50667245	50667241	ID=Msa1023310-mRNA-1:cds;Parent=Msa1023310-mRNA-1
chr7_1	CDS	50673311	50673530	50673311	ID=Msa1023310-mRNA-1:cds;Parent=Msa1023310-mRNA-1
chr7_1	CDS	50674671	50674886	50674671	ID=Msa1023310-mRNA-1:cds;Parent=Msa1023310-mRNA-1
chr7_1	CDS	50678905	50679138	50678905	ID=Msa1023310-mRNA-1:cds;Parent=Msa1023310-mRNA-1
chr7_1	CDS	50679676	50680029	50679676	ID=Msa1023310-mRNA-1:cds;Parent=Msa1023310-mRNA-1
chr7_1	three_prime_UTR	50680030	50680314	50680030	ID=Msa1023310-mRNA-1:three_prime_utr;Parent=Msa1023310-mRNA-1

Gene Sequence

>Msa1023310

ATGGTCGCTAAGATGGCATCAAATCAGGAAAATGTGAAGCTTTTTGGAATGATGGCAAGTCCATTTGTGAGCAGGGTGGAGATTGCTCTAACATTGAAGGGAGTTGAATACAAATATGAGTTAGAAAAGGGAGGGAACTTGAGTGATACACTCATAAAATACAACCCTGTTTACAAGAAGGTTCCTGTATTAGTTCATAATGATAAGCCTATATCAGAATCCCTTTGTTTAAGTACTGCACGGAAAGCTGCTTTCACTATTGATGAGAAAGAACGTGAGAAAGCTATTGAGGAAACAGCAGAGACTTTTCAAATTCTTGAGAATGAGCTCAAAGGAAAATTCTTTGGTGGAGAGGAGATTGGAATTGTTGATATAGCTGCAGTCTTCATAGCTTTTTGGTTCCCCATCGTACAAGAAGCAACAAACTTGAAATTTTCACTAGGTGTTGAATACAAATATGAGGAAGAAAAGCAGGGGAACTTGAGTGATACAGTCATTAAATACAACCCTGTTTATAAGAAAGTTCCTGTATTAGTTCATAATGGCAAACCTATATCAGAGTCACTTGTGATTCTTGAATATATTGATGAGACTTGGAAACAAAATCCAATCTTGCCTTCTGATCCTTATAAAAGAGCCTTGGCTCGTTTCTGGTCCAAGTTCATAGATGATAAGTGTATGATTCCTGCATGGAAAACTGCTTTCACTCTTGATGAGAAAGAACGTGAGAAGAGTTTTGAGGAAACAAAAGAGGCTTTTCAATTTCTTGAGAATGAGCTCAAGGGCAAGTTCTTTGGTGGAGATGAGATTGGACTTATTGACATTACTGCTGTCTTTATAGCTTTTTGGTTCCCTATTGTACAAGAAGTAACAGGACTCAAGTTATTCACAAGTGAGAAATTTCCTAAGCTTTACAATTGGAGCCAAGAATTCAACAACCATCCAATTGTCAAGGAAAATTTGCCTCGTAGAGAAGGGCTTTTGGCCTTCTTCAAAGCTCGTTATGAAAGTCTTGTTGCTTCAAAATAG

Protein sequence

>Msa1023310

MVAKMASNQENVKLFGMMASPFVSRVEIALTLKGVEYKYELEKGGNLSDTLIKYNPVYKKVPVLVHNDKPISESLCLSTARKAAFTIDEKEREKAIEETAETFQILENELKGKFFGGEEIGIVDIAAVFIAFWFPIVQEATNLKFSLGVEYKYEEEKQGNLSDTVIKYNPVYKKVPVLVHNGKPISESLVILEYIDETWKQNPILPSDPYKRALARFWSKFIDDKCMIPAWKTAFTLDEKEREKSFEETKEAFQFLENELKGKFFGGDEIGLIDITAVFIAFWFPIVQEVTGLKLFTSEKFPKLYNWSQEFNNHPIVKENLPRREGLLAFFKARYESLVASK*