AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0188870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188870 MtrunA17_Chr2g0277991 99.203 251 2 0 18 268 6 256 0.0 512
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188870 AT1G10780.2 60.000 250 100 0 19 268 5 254 5.63e-106 315
Msa0188870 AT1G10780.1 60.484 248 98 0 21 268 1 248 1.56e-105 314

Find 132 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATGAAGATCTGAAATT+AGG 0.132240 2_1:-4090594 None:intergenic
TTCTTCAAATGCTTGGTTTC+AGG 0.149041 2_1:-4092188 None:intergenic
TTATGGAACGTTTCCCATTT+AGG 0.175370 2_1:-4091918 None:intergenic
TGATCAAACTTATCCCTAAA+TGG 0.192449 2_1:+4091904 Msa0188870:CDS
CTTGAATCATTGGTAAAGTA+TGG 0.210999 2_1:+4092950 Msa0188870:three_prime_UTR
AGAGTATACATGATTGATTT+TGG 0.231137 2_1:+4092377 Msa0188870:three_prime_UTR
AACCAAACCTTTGATTCTTT+TGG 0.254388 2_1:-4093367 None:intergenic
AAATCAACAAAATCAATTTC+TGG 0.256476 2_1:-4092548 None:intergenic
CTGTGTATGGCCTTGCTTCA+TGG 0.264549 2_1:+4091736 Msa0188870:CDS
TTTACTTGTTAATTGCTTGC+AGG 0.266377 2_1:+4092353 Msa0188870:three_prime_UTR
TATGGAACGTTTCCCATTTA+GGG 0.268647 2_1:-4091917 None:intergenic
CTTCAAGGATCCTAAGATTA+TGG 0.284602 2_1:-4091935 None:intergenic
TTGAATTCAGATTTGGCGTT+TGG 0.292412 2_1:+4090948 Msa0188870:intron
GCTTCATGGCTATCACTTGC+AGG 0.307648 2_1:+4091750 Msa0188870:CDS
GGGAAACGTTCCATAATCTT+AGG 0.315212 2_1:+4091925 Msa0188870:CDS
TGAATTCAGATTTGGCGTTT+GGG 0.327339 2_1:+4090949 Msa0188870:intron
TGAATTTGTGATTCCATGTT+TGG 0.332405 2_1:+4092871 Msa0188870:three_prime_UTR
AGTAGCAACCTTGTCAGATT+TGG 0.335278 2_1:-4092011 None:intergenic
ACAACAAATGATCATTGCTT+TGG 0.342019 2_1:-4093470 None:intergenic
TTCAGAAGTCTAGCCGTAAC+TGG 0.343031 2_1:-4093142 None:intergenic
TTAGGATCCTTGAAGTTGTT+GGG 0.351315 2_1:+4091943 Msa0188870:CDS
AAAATGAAGATCTGAAATTA+GGG 0.353802 2_1:-4090593 None:intergenic
TCAAGATTATGTTACACTTT+CGG 0.366892 2_1:+4093405 Msa0188870:three_prime_UTR
TGCAGTAAATGCAATGATTC+AGG 0.369212 2_1:+4091983 Msa0188870:CDS
TACAAGGCTGTAGTTGGATT+AGG 0.380330 2_1:+4092162 Msa0188870:CDS
AAGCTCTTGGTGTGTGTTGT+TGG 0.381446 2_1:-4090561 None:intergenic
CTTAGGATCCTTGAAGTTGT+TGG 0.389083 2_1:+4091942 Msa0188870:CDS
TTTATTGCTTGTCGATGCCT+TGG 0.396042 2_1:+4093506 Msa0188870:three_prime_UTR
TCTTCAAATGCTTGGTTTCA+GGG 0.396771 2_1:-4092187 None:intergenic
CAACAAATGATCATTGCTTT+GGG 0.397588 2_1:-4093469 None:intergenic
AATGAGCACTCTTGAATCAT+TGG 0.400416 2_1:+4092940 Msa0188870:three_prime_UTR
CCAATGCAATCCAACTTTGA+TGG 0.404251 2_1:-4091840 None:intergenic
TACTGCCCTTTCTCTGTGTA+TGG 0.406508 2_1:+4091723 Msa0188870:CDS
TCTTGAATAAGCTTGATTGT+AGG 0.409281 2_1:-4093338 None:intergenic
TGAAAGATTCTTCAAATGCT+TGG 0.409351 2_1:-4092195 None:intergenic
CTCTTGCCCCAACAACTTCA+AGG 0.412272 2_1:-4091950 None:intergenic
TTATGTTACACTTTCGGAAA+AGG 0.412378 2_1:+4093411 Msa0188870:three_prime_UTR
AAGGCATCGACAAGCAATAA+AGG 0.420147 2_1:-4093504 None:intergenic
GACCAAAAGAATCAAAGGTT+TGG 0.420514 2_1:+4093365 Msa0188870:three_prime_UTR
GATCAAACTTATCCCTAAAT+GGG 0.424364 2_1:+4091905 Msa0188870:CDS
TCTTGGAAATTTATGCTACA+TGG 0.426045 2_1:+4093307 Msa0188870:three_prime_UTR
ACAAGGCTGTAGTTGGATTA+GGG 0.427712 2_1:+4092163 Msa0188870:CDS
CATTCGACTACCTGCACTGT+TGG 0.430763 2_1:-4092222 None:intergenic
ACAAAATCAATTTCTGGAAA+GGG 0.432172 2_1:-4092542 None:intergenic
TATCAAGATTCTTCAGATGA+AGG 0.439513 2_1:+4092994 Msa0188870:three_prime_UTR
TATGTTACACTTTCGGAAAA+GGG 0.440873 2_1:+4093412 Msa0188870:three_prime_UTR
AACAAAATCAATTTCTGGAA+AGG 0.445993 2_1:-4092543 None:intergenic
GAAGATCTGAAATTAGGGTT+AGG 0.448228 2_1:-4090588 None:intergenic
TTATTGCTTGTCGATGCCTT+GGG 0.450150 2_1:+4093507 Msa0188870:three_prime_UTR
CACGGTGCATGTATCTGAAC+CGG 0.452753 2_1:-4093057 None:intergenic
TATATGAAGGTTGAATTCAC+CGG 0.457387 2_1:+4092500 Msa0188870:three_prime_UTR
AGGGTTAGGTTACAAGCTCT+TGG 0.459613 2_1:-4090574 None:intergenic
ACATGAAAGCCCATCAAAGT+TGG 0.466038 2_1:+4091830 Msa0188870:CDS
ATATCAAACTTGCGCAGCTT+GGG 0.468187 2_1:-4092584 None:intergenic
AGCTCTTGGTGTGTGTTGTT+GGG 0.468906 2_1:-4090560 None:intergenic
CTGGCATAGTGCTGCAAACA+TGG 0.469002 2_1:-4092615 None:intergenic
GCAAGGCCATACACAGAGAA+AGG 0.474113 2_1:-4091729 None:intergenic
TACAATCAAGCTTATTCAAG+AGG 0.477254 2_1:+4093340 Msa0188870:three_prime_UTR
ATGTGGAAACTCTAAAATTG+TGG 0.481641 2_1:+4091874 Msa0188870:CDS
AGGTCCGTCTCTCTCTCATC+TGG 0.487819 2_1:+4091770 Msa0188870:CDS
GATATCAAACTTGCGCAGCT+TGG 0.490212 2_1:-4092585 None:intergenic
CTCTCATCTGGAGCTTCGAA+TGG 0.496250 2_1:+4091782 Msa0188870:CDS
TTATCTAATTCAGAGAATGA+AGG 0.497659 2_1:+4093239 Msa0188870:three_prime_UTR
CGATGACTTCCTTGCAAAGA+TGG 0.498978 2_1:+4093443 Msa0188870:three_prime_UTR
TTACTTGTTAATTGCTTGCA+GGG 0.499651 2_1:+4092354 Msa0188870:three_prime_UTR
TCGAGGTACAAGGCTGTAGT+TGG 0.501402 2_1:+4092156 Msa0188870:CDS
ATGTACTGAAGTATGACATC+AGG 0.505612 2_1:-4091504 None:intergenic
ATCAGCCGTTGAGAGATTAG+AGG 0.507988 2_1:+4091689 Msa0188870:CDS
CTTTCAATTTCCAACAGTGC+AGG 0.508430 2_1:+4092212 Msa0188870:CDS
AATAAGCTTGATTGTAGGCA+TGG 0.511461 2_1:-4093333 None:intergenic
CAACCTTGTCAGATTTGGAC+AGG 0.511798 2_1:-4092006 None:intergenic
CAATGCAATCCAACTTTGAT+GGG 0.512313 2_1:-4091839 None:intergenic
CCATCAAAGTTGGATTGCAT+TGG 0.513791 2_1:+4091840 Msa0188870:CDS
CAAGGCCATACACAGAGAAA+GGG 0.514970 2_1:-4091728 None:intergenic
TGACATCATTGTGAAAAGAA+TGG 0.515415 2_1:+4091665 Msa0188870:CDS
AACAAGACAAAGAGAAACTA+GGG 0.515915 2_1:-4093552 None:intergenic
ATCAAGATTCTTCAGATGAA+GGG 0.518042 2_1:+4092995 Msa0188870:three_prime_UTR
CTGCGCAAGTTTGATATCCA+TGG 0.520959 2_1:+4092590 Msa0188870:three_prime_UTR
AGTACAGTAAAACAATGTCT+TGG 0.524086 2_1:+4093290 Msa0188870:three_prime_UTR
TATCTAATTCAGAGAATGAA+GGG 0.524169 2_1:+4093240 Msa0188870:three_prime_UTR
CCCTGGAGTTTCTGTCAATG+AGG 0.528486 2_1:+4092411 Msa0188870:three_prime_UTR
CCCTCATTGACAGAAACTCC+AGG 0.531655 2_1:-4092412 None:intergenic
CTGACAAGGTTGCTACTGCT+TGG 0.532871 2_1:+4092017 Msa0188870:CDS
TGTGGAAACTCTAAAATTGT+GGG 0.532904 2_1:+4091875 Msa0188870:CDS
TTCGTACTGATTTCCAGTTA+CGG 0.533926 2_1:+4093129 Msa0188870:three_prime_UTR
TGTGATTCCATGTTTGGAGG+AGG 0.535055 2_1:+4092877 Msa0188870:three_prime_UTR
TTGAATCATTGGTAAAGTAT+GGG 0.539200 2_1:+4092951 Msa0188870:three_prime_UTR
GAATTCAGATTTGGCGTTTG+GGG 0.542302 2_1:+4090950 Msa0188870:intron
AAACAAGACAAAGAGAAACT+AGG 0.551859 2_1:-4093553 None:intergenic
TTGCAAGGAAGTCATCGCAT+TGG 0.553807 2_1:-4093437 None:intergenic
AGGGAGACCAAAAGAATCAA+AGG 0.556544 2_1:+4093360 Msa0188870:three_prime_UTR
TAGGATCCTTGAAGTTGTTG+GGG 0.559049 2_1:+4091944 Msa0188870:CDS
TGTGAAACATCTTTATATGA+AGG 0.559278 2_1:+4092487 Msa0188870:three_prime_UTR
GCAATTGTGTTTCTAAGCGA+TGG 0.559701 2_1:+4091565 Msa0188870:CDS
ATGAAGACCTCCTCCAAACA+TGG 0.559835 2_1:-4092884 None:intergenic
ATTGAATCTCTCGAGGTACA+AGG 0.560371 2_1:+4092146 Msa0188870:CDS
CCTGGAGTTTCTGTCAATGA+GGG 0.562266 2_1:+4092412 Msa0188870:three_prime_UTR
CAAACTTGCGCAGCTTGGGA+TGG 0.564636 2_1:-4092580 None:intergenic
TTGCTACTGCTTGGATGTGA+AGG 0.570826 2_1:+4092026 Msa0188870:CDS
GTGCTGCAAACATGGCTCCA+TGG 0.570847 2_1:-4092607 None:intergenic
ACAGAAACTCCAGGGATGCA+AGG 0.571452 2_1:-4092403 None:intergenic
ATATGAAGGTTGAATTCACC+GGG 0.576078 2_1:+4092501 Msa0188870:three_prime_UTR
GCAGGTTGATCGAAATTGAT+CGG 0.581191 2_1:-4092052 None:intergenic
AATTCAGATTTGGCGTTTGG+GGG 0.585966 2_1:+4090951 Msa0188870:intron
ATATTGTCACATGTCAGTAA+TGG 0.592308 2_1:+4091525 Msa0188870:CDS
GAGCTTCGAATGGACAACCT+TGG 0.594522 2_1:+4091792 Msa0188870:CDS
ATTCTATTCGAATTATCCCA+CGG 0.597758 2_1:-4093075 None:intergenic
GTATAATCTCATTATCGCCA+AGG 0.600599 2_1:-4091809 None:intergenic
GGCAGAGATTCGATTTCTGT+AGG 0.601489 2_1:-4091483 None:intergenic
TTGAAGAGCATCATAGTCCC+CGG 0.602248 2_1:-4092519 None:intergenic
TTTGGGCACCCATCTTTGCA+AGG 0.608811 2_1:-4093452 None:intergenic
ATTTGTGATTCCATGTTTGG+AGG 0.609528 2_1:+4092874 Msa0188870:three_prime_UTR
CCCTAAAATTGAATCTCTCG+AGG 0.610186 2_1:+4092139 Msa0188870:CDS
AGTTGTTGGGGCAAGAGTCG+AGG 0.613896 2_1:+4091956 Msa0188870:CDS
CAAGTGATAGCCATGAAGCA+AGG 0.624718 2_1:-4091746 None:intergenic
AAAGAAAATTGAGCTTACAA+AGG 0.627242 2_1:-4092329 None:intergenic
GGTTCAGATACATGCACCGT+GGG 0.628609 2_1:+4093059 Msa0188870:three_prime_UTR
ACAATCAAGCTTATTCAAGA+GGG 0.630838 2_1:+4093341 Msa0188870:three_prime_UTR
CTGAAGAATCTTGATAACCA+TGG 0.631753 2_1:-4092987 None:intergenic
AGCTCCAGATGAGAGAGAGA+CGG 0.634676 2_1:-4091774 None:intergenic
CTGGAGTTTCTGTCAATGAG+GGG 0.644439 2_1:+4092413 Msa0188870:three_prime_UTR
GATGACTTCCTTGCAAAGAT+GGG 0.646616 2_1:+4093444 Msa0188870:three_prime_UTR
TAGCTCCTCTAATCTCTCAA+CGG 0.648817 2_1:-4091694 None:intergenic
TGGAGTTTCTGTCAATGAGG+GGG 0.656210 2_1:+4092414 Msa0188870:three_prime_UTR
CCTCATTGACAGAAACTCCA+GGG 0.657818 2_1:-4092411 None:intergenic
AATTGCAATATGCAACATCA+CGG 0.658583 2_1:-4091548 None:intergenic
AGGCCTGTCCAAATCTGACA+AGG 0.667331 2_1:+4092003 Msa0188870:CDS
AGAGATTCGATTTCTGTAGG+CGG 0.681192 2_1:-4091480 None:intergenic
CATTGGTGCAGCAGTCAATG+TGG 0.696850 2_1:+4091857 Msa0188870:CDS
CGGTTCAGATACATGCACCG+TGG 0.706295 2_1:+4093058 Msa0188870:three_prime_UTR
GTGGAAACTCTAAAATTGTG+GGG 0.713865 2_1:+4091876 Msa0188870:CDS
TATGAAGGTTGAATTCACCG+GGG 0.788676 2_1:+4092502 Msa0188870:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATGAAGATCTGAAATTA+GGG - chr2_1:4090596-4090615 None:intergenic 20.0%
!!! ATGTTTTTTGAATTCAGATT+TGG + chr2_1:4090941-4090960 Msa0188870:intron 20.0%
!! TTACTCATATATTGCTAATT+TGG + chr2_1:4091157-4091176 Msa0188870:intron 20.0%
!! TTGTTTAAGTACAAAAGAAT+TGG + chr2_1:4091217-4091236 Msa0188870:intron 20.0%
!! TGTTTAAGTACAAAAGAATT+GGG + chr2_1:4091218-4091237 Msa0188870:intron 20.0%
!! CTATTTGTATATTGCAAATT+TGG + chr2_1:4091386-4091405 Msa0188870:intron 20.0%
!! AAATCAACAAAATCAATTTC+TGG - chr2_1:4092551-4092570 None:intergenic 20.0%
!! ATATTATAACCATGATTTCA+AGG + chr2_1:4092696-4092715 Msa0188870:intron 20.0%
!!! AAGATATCCATTTATTTTAC+AGG + chr2_1:4092841-4092860 Msa0188870:intron 20.0%
!! GAAAATGAAGATCTGAAATT+AGG - chr2_1:4090597-4090616 None:intergenic 25.0%
! TATAGTGCTTAGATCTTAAT+TGG + chr2_1:4090784-4090803 Msa0188870:intron 25.0%
! ATAGTGCTTAGATCTTAATT+GGG + chr2_1:4090785-4090804 Msa0188870:intron 25.0%
! AATAAAACCTAGATTGGAAA+AGG - chr2_1:4090846-4090865 None:intergenic 25.0%
! GTGATAATTAAGATAGTTAG+AGG + chr2_1:4091026-4091045 Msa0188870:intron 25.0%
!! TGTTAGAGTAATTTTATTCG+AGG + chr2_1:4091070-4091089 Msa0188870:intron 25.0%
! TGTACTTAAACAATACTAAC+CGG - chr2_1:4091210-4091229 None:intergenic 25.0%
! AAAGAAAATTGAGCTTACAA+AGG - chr2_1:4092332-4092351 None:intergenic 25.0%
!!! AGAGTATACATGATTGATTT+TGG + chr2_1:4092377-4092396 Msa0188870:three_prime_UTR 25.0%
! TGTGAAACATCTTTATATGA+AGG + chr2_1:4092487-4092506 Msa0188870:three_prime_UTR 25.0%
!! ACAAAATCAATTTCTGGAAA+GGG - chr2_1:4092545-4092564 None:intergenic 25.0%
!! AACAAAATCAATTTCTGGAA+AGG - chr2_1:4092546-4092565 None:intergenic 25.0%
! GAATCAACCTGTAAAATAAA+TGG - chr2_1:4092851-4092870 None:intergenic 25.0%
!! ATTTTCTGTTCAGCATTTAA+TGG - chr2_1:4092923-4092942 None:intergenic 25.0%
! TTGAATCATTGGTAAAGTAT+GGG + chr2_1:4092951-4092970 Msa0188870:three_prime_UTR 25.0%
! TAAAGTATGGGAAAAATCTA+AGG + chr2_1:4092963-4092982 Msa0188870:three_prime_UTR 25.0%
!!! ATTTTTTTGATGAGATTTGC+CGG + chr2_1:4093038-4093057 Msa0188870:three_prime_UTR 25.0%
!! TTGATGCAATTTTTAACACA+GGG + chr2_1:4093103-4093122 Msa0188870:three_prime_UTR 25.0%
!!! TGATAATTAGTTCACTTTTG+TGG + chr2_1:4093166-4093185 Msa0188870:three_prime_UTR 25.0%
! TTATCTAATTCAGAGAATGA+AGG + chr2_1:4093239-4093258 Msa0188870:three_prime_UTR 25.0%
! TATCTAATTCAGAGAATGAA+GGG + chr2_1:4093240-4093259 Msa0188870:three_prime_UTR 25.0%
! TCAAGATTATGTTACACTTT+CGG + chr2_1:4093405-4093424 Msa0188870:three_prime_UTR 25.0%
CAGCTAAGTTAAAGCAAAAA+GGG - chr2_1:4090674-4090693 None:intergenic 30.0%
GCAAAGAATAAAACCTAGAT+TGG - chr2_1:4090852-4090871 None:intergenic 30.0%
! GAGGGTAATTTGAAGTAAAT+GGG + chr2_1:4090984-4091003 Msa0188870:intron 30.0%
AAACAATACTAACCGGTATA+TGG - chr2_1:4091203-4091222 None:intergenic 30.0%
! GTATGATTTGAAGTCGAAAT+CGG - chr2_1:4091365-4091384 None:intergenic 30.0%
ATATTGTCACATGTCAGTAA+TGG + chr2_1:4091525-4091544 Msa0188870:CDS 30.0%
AATTGCAATATGCAACATCA+CGG - chr2_1:4091551-4091570 None:intergenic 30.0%
! CAATGATGTCAGAATTTTCA+CGG - chr2_1:4091656-4091675 None:intergenic 30.0%
TGACATCATTGTGAAAAGAA+TGG + chr2_1:4091665-4091684 Msa0188870:CDS 30.0%
!! ATGTGGAAACTCTAAAATTG+TGG + chr2_1:4091874-4091893 Msa0188870:CDS 30.0%
!! TGTGGAAACTCTAAAATTGT+GGG + chr2_1:4091875-4091894 Msa0188870:CDS 30.0%
TGATCAAACTTATCCCTAAA+TGG + chr2_1:4091904-4091923 Msa0188870:CDS 30.0%
GATCAAACTTATCCCTAAAT+GGG + chr2_1:4091905-4091924 Msa0188870:CDS 30.0%
TGAAAGATTCTTCAAATGCT+TGG - chr2_1:4092198-4092217 None:intergenic 30.0%
GGAGAGCAATAAAAAAACAA+AGG - chr2_1:4092285-4092304 None:intergenic 30.0%
!!! TTGTTTTTTTATTGCTCTCC+TGG + chr2_1:4092285-4092304 Msa0188870:CDS 30.0%
TCCTGGAATTTACTAAAAAG+TGG + chr2_1:4092302-4092321 Msa0188870:CDS 30.0%
!!! ACCACTTTTTAGTAAATTCC+AGG - chr2_1:4092306-4092325 None:intergenic 30.0%
! TTTACTTGTTAATTGCTTGC+AGG + chr2_1:4092353-4092372 Msa0188870:three_prime_UTR 30.0%
! TTACTTGTTAATTGCTTGCA+GGG + chr2_1:4092354-4092373 Msa0188870:three_prime_UTR 30.0%
TATATGAAGGTTGAATTCAC+CGG + chr2_1:4092500-4092519 Msa0188870:three_prime_UTR 30.0%
TGAATTTGTGATTCCATGTT+TGG + chr2_1:4092871-4092890 Msa0188870:three_prime_UTR 30.0%
CTTGAATCATTGGTAAAGTA+TGG + chr2_1:4092950-4092969 Msa0188870:three_prime_UTR 30.0%
TATCAAGATTCTTCAGATGA+AGG + chr2_1:4092994-4093013 Msa0188870:three_prime_UTR 30.0%
ATCAAGATTCTTCAGATGAA+GGG + chr2_1:4092995-4093014 Msa0188870:three_prime_UTR 30.0%
ATTCTATTCGAATTATCCCA+CGG - chr2_1:4093078-4093097 None:intergenic 30.0%
! CTTGATGCAATTTTTAACAC+AGG + chr2_1:4093102-4093121 Msa0188870:three_prime_UTR 30.0%
! ACTGTACTTTTGACAATCAT+GGG - chr2_1:4093278-4093297 None:intergenic 30.0%
! TACTGTACTTTTGACAATCA+TGG - chr2_1:4093279-4093298 None:intergenic 30.0%
AGTACAGTAAAACAATGTCT+TGG + chr2_1:4093290-4093309 Msa0188870:three_prime_UTR 30.0%
TCTTGGAAATTTATGCTACA+TGG + chr2_1:4093307-4093326 Msa0188870:three_prime_UTR 30.0%
TCTTGAATAAGCTTGATTGT+AGG - chr2_1:4093341-4093360 None:intergenic 30.0%
TACAATCAAGCTTATTCAAG+AGG + chr2_1:4093340-4093359 Msa0188870:three_prime_UTR 30.0%
ACAATCAAGCTTATTCAAGA+GGG + chr2_1:4093341-4093360 Msa0188870:three_prime_UTR 30.0%
! AACCAAACCTTTGATTCTTT+TGG - chr2_1:4093370-4093389 None:intergenic 30.0%
TTATGTTACACTTTCGGAAA+AGG + chr2_1:4093411-4093430 Msa0188870:three_prime_UTR 30.0%
TATGTTACACTTTCGGAAAA+GGG + chr2_1:4093412-4093431 Msa0188870:three_prime_UTR 30.0%
CAACAAATGATCATTGCTTT+GGG - chr2_1:4093472-4093491 None:intergenic 30.0%
ACAACAAATGATCATTGCTT+TGG - chr2_1:4093473-4093492 None:intergenic 30.0%
AACAAGACAAAGAGAAACTA+GGG - chr2_1:4093555-4093574 None:intergenic 30.0%
AAACAAGACAAAGAGAAACT+AGG - chr2_1:4093556-4093575 None:intergenic 30.0%
! GAAGATCTGAAATTAGGGTT+AGG - chr2_1:4090591-4090610 None:intergenic 35.0%
CCAGCTAAGTTAAAGCAAAA+AGG - chr2_1:4090675-4090694 None:intergenic 35.0%
!! CCTTTTTGCTTTAACTTAGC+TGG + chr2_1:4090672-4090691 Msa0188870:intron 35.0%
!! TGCATCCAATGTTCAAGTTT+TGG + chr2_1:4090737-4090756 Msa0188870:intron 35.0%
!! GCATCCAATGTTCAAGTTTT+GGG + chr2_1:4090738-4090757 Msa0188870:intron 35.0%
TGAACCCAAAACTTGAACAT+TGG - chr2_1:4090745-4090764 None:intergenic 35.0%
! AAACTCACCTTTTCCAATCT+AGG + chr2_1:4090836-4090855 Msa0188870:intron 35.0%
TTGAATTCAGATTTGGCGTT+TGG + chr2_1:4090948-4090967 Msa0188870:intron 35.0%
TGAATTCAGATTTGGCGTTT+GGG + chr2_1:4090949-4090968 Msa0188870:intron 35.0%
! GGAGGGTAATTTGAAGTAAA+TGG + chr2_1:4090983-4091002 Msa0188870:intron 35.0%
TAAATGGGAGCTAAACTCAA+AGG + chr2_1:4090999-4091018 Msa0188870:intron 35.0%
AAATGGGAGCTAAACTCAAA+GGG + chr2_1:4091000-4091019 Msa0188870:intron 35.0%
TATTTGATCCGTCCATATAC+CGG + chr2_1:4091188-4091207 Msa0188870:intron 35.0%
AATACTAACCGGTATATGGA+CGG - chr2_1:4091199-4091218 None:intergenic 35.0%
ATGTACTGAAGTATGACATC+AGG - chr2_1:4091507-4091526 None:intergenic 35.0%
GTATAATCTCATTATCGCCA+AGG - chr2_1:4091812-4091831 None:intergenic 35.0%
CAATGCAATCCAACTTTGAT+GGG - chr2_1:4091842-4091861 None:intergenic 35.0%
!! GTGGAAACTCTAAAATTGTG+GGG + chr2_1:4091876-4091895 Msa0188870:CDS 35.0%
TATGGAACGTTTCCCATTTA+GGG - chr2_1:4091920-4091939 None:intergenic 35.0%
TTATGGAACGTTTCCCATTT+AGG - chr2_1:4091921-4091940 None:intergenic 35.0%
CTTCAAGGATCCTAAGATTA+TGG - chr2_1:4091938-4091957 None:intergenic 35.0%
TTAGGATCCTTGAAGTTGTT+GGG + chr2_1:4091943-4091962 Msa0188870:CDS 35.0%
TGCAGTAAATGCAATGATTC+AGG + chr2_1:4091983-4092002 Msa0188870:CDS 35.0%
! AAGCTGGATTTTTATGGTCT+TGG + chr2_1:4092092-4092111 Msa0188870:CDS 35.0%
! AGCTGGATTTTTATGGTCTT+GGG + chr2_1:4092093-4092112 Msa0188870:CDS 35.0%
! CTCGAGAGATTCAATTTTAG+GGG - chr2_1:4092141-4092160 None:intergenic 35.0%
! CCTCGAGAGATTCAATTTTA+GGG - chr2_1:4092142-4092161 None:intergenic 35.0%
! ACCTCGAGAGATTCAATTTT+AGG - chr2_1:4092143-4092162 None:intergenic 35.0%
TCTTCAAATGCTTGGTTTCA+GGG - chr2_1:4092190-4092209 None:intergenic 35.0%
TTCTTCAAATGCTTGGTTTC+AGG - chr2_1:4092191-4092210 None:intergenic 35.0%
ATATGAAGGTTGAATTCACC+GGG + chr2_1:4092501-4092520 Msa0188870:three_prime_UTR 35.0%
!! CATGTTTTAGACTGTTCTTC+TGG - chr2_1:4092637-4092656 None:intergenic 35.0%
! AAGCTACACACTATAGCTTT+TGG + chr2_1:4092719-4092738 Msa0188870:intron 35.0%
! TACACACTATAGCTTTTGGA+AGG + chr2_1:4092723-4092742 Msa0188870:intron 35.0%
ATTTGTGATTCCATGTTTGG+AGG + chr2_1:4092874-4092893 Msa0188870:three_prime_UTR 35.0%
AATGAGCACTCTTGAATCAT+TGG + chr2_1:4092940-4092959 Msa0188870:three_prime_UTR 35.0%
! CTGAAGAATCTTGATAACCA+TGG - chr2_1:4092990-4093009 None:intergenic 35.0%
!! TTCGTACTGATTTCCAGTTA+CGG + chr2_1:4093129-4093148 Msa0188870:three_prime_UTR 35.0%
! ACTTTTGACAATCATGGGAA+AGG - chr2_1:4093273-4093292 None:intergenic 35.0%
AATAAGCTTGATTGTAGGCA+TGG - chr2_1:4093336-4093355 None:intergenic 35.0%
GACCAAAAGAATCAAAGGTT+TGG + chr2_1:4093365-4093384 Msa0188870:three_prime_UTR 35.0%
GAATTCAGATTTGGCGTTTG+GGG + chr2_1:4090950-4090969 Msa0188870:intron 40.0%
AATTCAGATTTGGCGTTTGG+GGG + chr2_1:4090951-4090970 Msa0188870:intron 40.0%
!! TTTGGGGGTGTTAATTTGGA+GGG + chr2_1:4090966-4090985 Msa0188870:CDS 40.0%
AATTGGGAGCTAAACTGAGA+AGG + chr2_1:4091234-4091253 Msa0188870:intron 40.0%
ATTGGGAGCTAAACTGAGAA+GGG + chr2_1:4091235-4091254 Msa0188870:intron 40.0%
TGCGAGAGTTAGATGATTTG+AGG + chr2_1:4091304-4091323 Msa0188870:intron 40.0%
ATTCTGATGCTTGTGCATGA+CGG + chr2_1:4091337-4091356 Msa0188870:intron 40.0%
AGAGATTCGATTTCTGTAGG+CGG - chr2_1:4091483-4091502 None:intergenic 40.0%
! GCAATTGTGTTTCTAAGCGA+TGG + chr2_1:4091565-4091584 Msa0188870:CDS 40.0%
! ATGTCAGAATTTTCACGGTG+AGG - chr2_1:4091651-4091670 None:intergenic 40.0%
TAGCTCCTCTAATCTCTCAA+CGG - chr2_1:4091697-4091716 None:intergenic 40.0%
ACATGAAAGCCCATCAAAGT+TGG + chr2_1:4091830-4091849 Msa0188870:CDS 40.0%
CCAATGCAATCCAACTTTGA+TGG - chr2_1:4091843-4091862 None:intergenic 40.0%
CCATCAAAGTTGGATTGCAT+TGG + chr2_1:4091840-4091859 Msa0188870:CDS 40.0%
GGGAAACGTTCCATAATCTT+AGG + chr2_1:4091925-4091944 Msa0188870:CDS 40.0%
CTTAGGATCCTTGAAGTTGT+TGG + chr2_1:4091942-4091961 Msa0188870:CDS 40.0%
TAGGATCCTTGAAGTTGTTG+GGG + chr2_1:4091944-4091963 Msa0188870:CDS 40.0%
AGTAGCAACCTTGTCAGATT+TGG - chr2_1:4092014-4092033 None:intergenic 40.0%
!! GCAGGTTGATCGAAATTGAT+CGG - chr2_1:4092055-4092074 None:intergenic 40.0%
!!! TTCGATCAACCTGCCATTTT+TGG + chr2_1:4092061-4092080 Msa0188870:CDS 40.0%
! CAGTGCAAGCTGGATTTTTA+TGG + chr2_1:4092086-4092105 Msa0188870:CDS 40.0%
!! GAGAGATTCAATTTTAGGGG+AGG - chr2_1:4092138-4092157 None:intergenic 40.0%
CCCTAAAATTGAATCTCTCG+AGG + chr2_1:4092139-4092158 Msa0188870:CDS 40.0%
ATTGAATCTCTCGAGGTACA+AGG + chr2_1:4092146-4092165 Msa0188870:CDS 40.0%
TACAAGGCTGTAGTTGGATT+AGG + chr2_1:4092162-4092181 Msa0188870:CDS 40.0%
ACAAGGCTGTAGTTGGATTA+GGG + chr2_1:4092163-4092182 Msa0188870:CDS 40.0%
CTTTCAATTTCCAACAGTGC+AGG + chr2_1:4092212-4092231 Msa0188870:CDS 40.0%
TATGAAGGTTGAATTCACCG+GGG + chr2_1:4092502-4092521 Msa0188870:three_prime_UTR 40.0%
ATATCAAACTTGCGCAGCTT+GGG - chr2_1:4092587-4092606 None:intergenic 40.0%
GTGTAGCTTCCTTGAAATCA+TGG - chr2_1:4092708-4092727 None:intergenic 40.0%
!! CAGTTTGTTTTCTTCGACAC+AGG - chr2_1:4092750-4092769 None:intergenic 40.0%
CTGTGTCGAAGAAAACAAAC+TGG + chr2_1:4092748-4092767 Msa0188870:intron 40.0%
TGGGAAAAATCTAAGGACCA+TGG + chr2_1:4092970-4092989 Msa0188870:three_prime_UTR 40.0%
AGGGAGACCAAAAGAATCAA+AGG + chr2_1:4093360-4093379 Msa0188870:three_prime_UTR 40.0%
GATGACTTCCTTGCAAAGAT+GGG + chr2_1:4093444-4093463 Msa0188870:three_prime_UTR 40.0%
!! AAGGCATCGACAAGCAATAA+AGG - chr2_1:4093507-4093526 None:intergenic 40.0%
TTTATTGCTTGTCGATGCCT+TGG + chr2_1:4093506-4093525 Msa0188870:three_prime_UTR 40.0%
TTATTGCTTGTCGATGCCTT+GGG + chr2_1:4093507-4093526 Msa0188870:three_prime_UTR 40.0%
! GCCTTGGGCATAAGTTTTTA+AGG + chr2_1:4093522-4093541 Msa0188870:three_prime_UTR 40.0%
GCCTTAAAAACTTATGCCCA+AGG - chr2_1:4093526-4093545 None:intergenic 40.0%
! AGCTCTTGGTGTGTGTTGTT+GGG - chr2_1:4090563-4090582 None:intergenic 45.0%
! AAGCTCTTGGTGTGTGTTGT+TGG - chr2_1:4090564-4090583 None:intergenic 45.0%
AGGGTTAGGTTACAAGCTCT+TGG - chr2_1:4090577-4090596 None:intergenic 45.0%
!! GTTTGGGGGTGTTAATTTGG+AGG + chr2_1:4090965-4090984 Msa0188870:CDS 45.0%
GGCAGAGATTCGATTTCTGT+AGG - chr2_1:4091486-4091505 None:intergenic 45.0%
ATCAGCCGTTGAGAGATTAG+AGG + chr2_1:4091689-4091708 Msa0188870:CDS 45.0%
TACTGCCCTTTCTCTGTGTA+TGG + chr2_1:4091723-4091742 Msa0188870:CDS 45.0%
CAAGGCCATACACAGAGAAA+GGG - chr2_1:4091731-4091750 None:intergenic 45.0%
CAAGTGATAGCCATGAAGCA+AGG - chr2_1:4091749-4091768 None:intergenic 45.0%
CAACCTTGTCAGATTTGGAC+AGG - chr2_1:4092009-4092028 None:intergenic 45.0%
TTGCTACTGCTTGGATGTGA+AGG + chr2_1:4092026-4092045 Msa0188870:CDS 45.0%
AGCTTGCACTGCTCCAAAAA+TGG - chr2_1:4092077-4092096 None:intergenic 45.0%
!!! ATTTTTGGAGCAGTGCAAGC+TGG + chr2_1:4092076-4092095 Msa0188870:CDS 45.0%
!! TTTTGGAAACCTTGCATCCC+TGG + chr2_1:4092394-4092413 Msa0188870:three_prime_UTR 45.0%
CCTCATTGACAGAAACTCCA+GGG - chr2_1:4092414-4092433 None:intergenic 45.0%
CCTGGAGTTTCTGTCAATGA+GGG + chr2_1:4092412-4092431 Msa0188870:three_prime_UTR 45.0%
CTGGAGTTTCTGTCAATGAG+GGG + chr2_1:4092413-4092432 Msa0188870:three_prime_UTR 45.0%
TGGAGTTTCTGTCAATGAGG+GGG + chr2_1:4092414-4092433 Msa0188870:three_prime_UTR 45.0%
!! TTGAAGAGCATCATAGTCCC+CGG - chr2_1:4092522-4092541 None:intergenic 45.0%
GATATCAAACTTGCGCAGCT+TGG - chr2_1:4092588-4092607 None:intergenic 45.0%
CTGCGCAAGTTTGATATCCA+TGG + chr2_1:4092590-4092609 Msa0188870:three_prime_UTR 45.0%
TGTGATTCCATGTTTGGAGG+AGG + chr2_1:4092877-4092896 Msa0188870:three_prime_UTR 45.0%
ATGAAGACCTCCTCCAAACA+TGG - chr2_1:4092887-4092906 None:intergenic 45.0%
TTCAGAAGTCTAGCCGTAAC+TGG - chr2_1:4093145-4093164 None:intergenic 45.0%
TTGCAAGGAAGTCATCGCAT+TGG - chr2_1:4093440-4093459 None:intergenic 45.0%
CGATGACTTCCTTGCAAAGA+TGG + chr2_1:4093443-4093462 Msa0188870:three_prime_UTR 45.0%
!! GGCGTTTGGGGGTGTTAATT+TGG + chr2_1:4090962-4090981 Msa0188870:CDS 50.0%
GCAAGGCCATACACAGAGAA+AGG - chr2_1:4091732-4091751 None:intergenic 50.0%
! CTGTGTATGGCCTTGCTTCA+TGG + chr2_1:4091736-4091755 Msa0188870:CDS 50.0%
GCTTCATGGCTATCACTTGC+AGG + chr2_1:4091750-4091769 Msa0188870:CDS 50.0%
AGCTCCAGATGAGAGAGAGA+CGG - chr2_1:4091777-4091796 None:intergenic 50.0%
! CTCTCATCTGGAGCTTCGAA+TGG + chr2_1:4091782-4091801 Msa0188870:CDS 50.0%
GAGCTTCGAATGGACAACCT+TGG + chr2_1:4091792-4091811 Msa0188870:CDS 50.0%
CATTGGTGCAGCAGTCAATG+TGG + chr2_1:4091857-4091876 Msa0188870:CDS 50.0%
CTCTTGCCCCAACAACTTCA+AGG - chr2_1:4091953-4091972 None:intergenic 50.0%
AGGCCTGTCCAAATCTGACA+AGG + chr2_1:4092003-4092022 Msa0188870:CDS 50.0%
CTGACAAGGTTGCTACTGCT+TGG + chr2_1:4092017-4092036 Msa0188870:CDS 50.0%
TGCACTGCTCCAAAAATGGC+AGG - chr2_1:4092073-4092092 None:intergenic 50.0%
TCGAGGTACAAGGCTGTAGT+TGG + chr2_1:4092156-4092175 Msa0188870:CDS 50.0%
CATTCGACTACCTGCACTGT+TGG - chr2_1:4092225-4092244 None:intergenic 50.0%
ACAGAAACTCCAGGGATGCA+AGG - chr2_1:4092406-4092425 None:intergenic 50.0%
CCCTGGAGTTTCTGTCAATG+AGG + chr2_1:4092411-4092430 Msa0188870:three_prime_UTR 50.0%
CCCTCATTGACAGAAACTCC+AGG - chr2_1:4092415-4092434 None:intergenic 50.0%
TGTCAATGAGGGGGATACAG+TGG + chr2_1:4092423-4092442 Msa0188870:three_prime_UTR 50.0%
CTGGCATAGTGCTGCAAACA+TGG - chr2_1:4092618-4092637 None:intergenic 50.0%
CACGGTGCATGTATCTGAAC+CGG - chr2_1:4093060-4093079 None:intergenic 50.0%
GGTTCAGATACATGCACCGT+GGG + chr2_1:4093059-4093078 Msa0188870:three_prime_UTR 50.0%
TTTGGGCACCCATCTTTGCA+AGG - chr2_1:4093455-4093474 None:intergenic 50.0%
AGGCGGAGATTCGATTGCTG+TGG - chr2_1:4091466-4091485 None:intergenic 55.0%
AGGTCCGTCTCTCTCTCATC+TGG + chr2_1:4091770-4091789 Msa0188870:CDS 55.0%
AGTTGTTGGGGCAAGAGTCG+AGG + chr2_1:4091956-4091975 Msa0188870:CDS 55.0%
TGAGGGGGATACAGTGGTGT+TGG + chr2_1:4092429-4092448 Msa0188870:three_prime_UTR 55.0%
GAGGGGGATACAGTGGTGTT+GGG + chr2_1:4092430-4092449 Msa0188870:three_prime_UTR 55.0%
CAAACTTGCGCAGCTTGGGA+TGG - chr2_1:4092583-4092602 None:intergenic 55.0%
GTGCTGCAAACATGGCTCCA+TGG - chr2_1:4092610-4092629 None:intergenic 55.0%
CGGTTCAGATACATGCACCG+TGG + chr2_1:4093058-4093077 Msa0188870:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 4090551 4093637 4090551 ID=Msa0188870;Name=Msa0188870
chr2_1 mRNA 4090551 4093637 4090551 ID=Msa0188870-mRNA-1;Parent=Msa0188870;Name=Msa0188870-mRNA-1;_AED=0.01;_eAED=0.01;_QI=86|0.33|0.75|0.75|1|0.75|4|1093|301
chr2_1 exon 4090551 4090643 4090551 ID=Msa0188870-mRNA-1:exon:1826;Parent=Msa0188870-mRNA-1
chr2_1 exon 4090958 4090987 4090958 ID=Msa0188870-mRNA-1:exon:1827;Parent=Msa0188870-mRNA-1
chr2_1 exon 4091469 4092655 4091469 ID=Msa0188870-mRNA-1:exon:1828;Parent=Msa0188870-mRNA-1
chr2_1 exon 4092863 4093637 4092863 ID=Msa0188870-mRNA-1:exon:1829;Parent=Msa0188870-mRNA-1
chr2_1 five_prime_UTR 4090551 4090636 4090551 ID=Msa0188870-mRNA-1:five_prime_utr;Parent=Msa0188870-mRNA-1
chr2_1 CDS 4090637 4090643 4090637 ID=Msa0188870-mRNA-1:cds;Parent=Msa0188870-mRNA-1
chr2_1 CDS 4090958 4090987 4090958 ID=Msa0188870-mRNA-1:cds;Parent=Msa0188870-mRNA-1
chr2_1 CDS 4091469 4092337 4091469 ID=Msa0188870-mRNA-1:cds;Parent=Msa0188870-mRNA-1
chr2_1 three_prime_UTR 4092338 4092655 4092338 ID=Msa0188870-mRNA-1:three_prime_utr;Parent=Msa0188870-mRNA-1
chr2_1 three_prime_UTR 4092863 4093637 4092863 ID=Msa0188870-mRNA-1:three_prime_utr;Parent=Msa0188870-mRNA-1
Gene Sequence

>Msa0188870

ATGCGTAATTTGGCGTTTGGGGGTGTTAATTTGGAGGCAATCGAATCTCCGCCTACAGAAATCGAATCTCTGCCTGATGTCATACTTCAGTACATATTGTCACATGTCAGTAATGGCCGTGATGTTGCATATTGCAATTGTGTTTCTAAGCGATGGAAGAACTCAATGACTTGTATAAGAAGTCTCTATTTTACTCGCAATGCGTTTGATAACGCTCCTCACCGTGAAAATTCTGACATCATTGTGAAAAGAATGGTATCAGCCGTTGAGAGATTAGAGGAGCTAGTTGTGTACTGCCCTTTCTCTGTGTATGGCCTTGCTTCATGGCTATCACTTGCAGGTCCGTCTCTCTCTCATCTGGAGCTTCGAATGGACAACCTTGGCGATAATGAGATTATACATGAAAGCCCATCAAAGTTGGATTGCATTGGTGCAGCAGTCAATGTGGAAACTCTAAAATTGTGGGGTGTCTTGATCAAACTTATCCCTAAATGGGAAACGTTCCATAATCTTAGGATCCTTGAAGTTGTTGGGGCAAGAGTCGAGGATGCTGCAGTAAATGCAATGATTCAGGCCTGTCCAAATCTGACAAGGTTGCTACTGCTTGGATGTGAAGGTGTCCGATCAATTTCGATCAACCTGCCATTTTTGGAGCAGTGCAAGCTGGATTTTTATGGTCTTGGGAACTGTTCGCTTTCACTGACCTCCCCTAAAATTGAATCTCTCGAGGTACAAGGCTGTAGTTGGATTAGGGTCCCTGAAACCAAGCATTTGAAGAATCTTTCAATTTCCAACAGTGCAGGTAGTCGAATGTTTGAAGTGATTCTTGTACTTTCATATTTCTTATTGACCTTTGTTTTTTTATTGCTCTCCTGGAATTTACTAAAAAGTGGTTTGCCTTTGTAA
Protein sequence

>Msa0188870

MRNLAFGGVNLEAIESPPTEIESLPDVILQYILSHVSNGRDVAYCNCVSKRWKNSMTCIRSLYFTRNAFDNAPHRENSDIIVKRMVSAVERLEELVVYCPFSVYGLASWLSLAGPSLSHLELRMDNLGDNEIIHESPSKLDCIGAAVNVETLKLWGVLIKLIPKWETFHNLRILEVVGARVEDAAVNAMIQACPNLTRLLLLGCEGVRSISINLPFLEQCKLDFYGLGNCSLSLTSPKIESLEVQGCSWIRVPETKHLKNLSISNSAGSRMFEVILVLSYFLLTFVFLLLSWNLLKSGLPL*