Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577330 | XP_003629630.2 | 90.831 | 1167 | 99 | 3 | 48 | 1208 | 1 | 1165 | 0.0 | 2100 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003080 | Msa0577330 | 0.800249 | 1.574504e-48 | -8.615850e-47 |
| Msa0005020 | Msa0577330 | 0.841470 | 4.661628e-58 | -8.615850e-47 |
| Msa0015570 | Msa0577330 | 0.831631 | 1.496200e-55 | -8.615850e-47 |
| Msa0016040 | Msa0577330 | 0.854406 | 1.257387e-61 | -8.615850e-47 |
| Msa0016530 | Msa0577330 | 0.817398 | 3.366150e-52 | -8.615850e-47 |
| Msa0018920 | Msa0577330 | 0.858971 | 5.710574e-63 | -8.615850e-47 |
| Msa0019930 | Msa0577330 | 0.845883 | 3.075346e-59 | -8.615850e-47 |
| Msa0020540 | Msa0577330 | 0.881662 | 1.992739e-70 | -8.615850e-47 |
| Msa0027800 | Msa0577330 | 0.829405 | 5.244883e-55 | -8.615850e-47 |
| Msa0030070 | Msa0577330 | 0.824725 | 6.908438e-54 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577330 | MtrunA17_Chr8g0377891 | 90.831 | 1167 | 99 | 3 | 48 | 1208 | 1 | 1165 | 0.0 | 2100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577330 | AT3G23740.1 | 33.724 | 341 | 179 | 9 | 895 | 1209 | 242 | 561 | 5.43e-37 | 148 |
Find 274 sgRNAs with CRISPR-Local
Find 429 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCCAAGAGGATGCAGA+GGG | 0.661927 | 4_1:-81631233 | Msa0577330:CDS |
| AAAAGGAACAGGAACGGGAA+CGG | 0.566531 | 4_1:+81635156 | None:intergenic |
| AAAAGGAAGCAATAACAAAA+AGG | 0.364259 | 4_1:-81632156 | Msa0577330:CDS |
| AAAAGGCTAATGGATGCTAT+GGG | 0.591299 | 4_1:-81634338 | Msa0577330:CDS |
| AAAGCCCTCTGCATCCTCTT+GGG | 0.411441 | 4_1:+81631229 | None:intergenic |
| AAAGCTCACCGTACCCGCTC+CGG | 0.482065 | 4_1:-81635280 | Msa0577330:CDS |
| AAAGGAGTTCTCGAGATAGT+AGG | 0.415123 | 4_1:+81631470 | None:intergenic |
| AAATCTCTATGAACAGAAAT+TGG | 0.285206 | 4_1:+81631441 | None:intergenic |
| AAATTGTATTGGCAATTCGG+AGG | 0.653709 | 4_1:+81632736 | None:intergenic |
| AACAATTTCAAAGGTAAACG+CGG | 0.645334 | 4_1:+81634386 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTTATATATGTGTGTGTGA+TGG | + | chr4_1:81629887-81629906 | None:intergenic | 30.0% | |
| TCAGCTGAAATTTCTGAATT+AGG | - | chr4_1:81630474-81630493 | Msa0577330:intron | 30.0% | |
| CAGCTGAAATTTCTGAATTA+GGG | - | chr4_1:81630475-81630494 | Msa0577330:intron | 30.0% | |
| GAAATTTCTGAATTAGGGTT+TGG | - | chr4_1:81630480-81630499 | Msa0577330:intron | 30.0% | |
| TTCAAAGGAGTAAATCTGTA+AGG | - | chr4_1:81630796-81630815 | Msa0577330:intron | 30.0% | |
| TTTCATCATTGCATATGTGT+TGG | - | chr4_1:81630834-81630853 | Msa0577330:intron | 30.0% | |
| AACAATTTCAAAGGTAAACG+CGG | + | chr4_1:81630936-81630955 | None:intergenic | 30.0% | |
| TTTGTAATTGAATGAACCTG+GGG | + | chr4_1:81630964-81630983 | None:intergenic | 30.0% | |
| CAGGTTCATTCAATTACAAA+AGG | - | chr4_1:81630964-81630983 | Msa0577330:intron | 30.0% | |
| TCATGATTATCAGATGTCAA+AGG | + | chr4_1:81631317-81631336 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 81629823 | 81635518 | 81629823 | ID=Msa0577330;Name=Msa0577330 |
| chr4_1 | mRNA | 81629823 | 81635518 | 81629823 | ID=Msa0577330-mRNA-1;Parent=Msa0577330;Name=Msa0577330-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.88|1|1|1|9|264|1212 |
| chr4_1 | exon | 81629823 | 81630167 | 81629823 | ID=Msa0577330-mRNA-1:exon:29390;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81630242 | 81630340 | 81630242 | ID=Msa0577330-mRNA-1:exon:29389;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81631041 | 81631738 | 81631041 | ID=Msa0577330-mRNA-1:exon:29388;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81631848 | 81631950 | 81631848 | ID=Msa0577330-mRNA-1:exon:29387;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81632106 | 81632992 | 81632106 | ID=Msa0577330-mRNA-1:exon:29386;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81633113 | 81633215 | 81633113 | ID=Msa0577330-mRNA-1:exon:29385;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81633506 | 81634092 | 81633506 | ID=Msa0577330-mRNA-1:exon:29384;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81634327 | 81634417 | 81634327 | ID=Msa0577330-mRNA-1:exon:29383;Parent=Msa0577330-mRNA-1 |
| chr4_1 | exon | 81634529 | 81635518 | 81634529 | ID=Msa0577330-mRNA-1:exon:29382;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81634529 | 81635518 | 81634529 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81634327 | 81634417 | 81634327 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81633506 | 81634092 | 81633506 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81633113 | 81633215 | 81633113 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81632106 | 81632992 | 81632106 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81631848 | 81631950 | 81631848 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81631041 | 81631738 | 81631041 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81630242 | 81630340 | 81630242 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | CDS | 81630087 | 81630167 | 81630087 | ID=Msa0577330-mRNA-1:cds;Parent=Msa0577330-mRNA-1 |
| chr4_1 | three_prime_UTR | 81629823 | 81630086 | 81629823 | ID=Msa0577330-mRNA-1:three_prime_utr;Parent=Msa0577330-mRNA-1 |
| Gene Sequence |
>Msa0577330 TTGGATTTTTATCAGAAATCACGCCACAAAATTTTCCCGCCAACAAAAACCCTTGTTCCCGCCATCACACACACATATATAAACAGCGCGCCAATTTTCACACATTTCGAGACCAAAGCAGCAGAGCAGAGCAGAGCAGCCATGGACGTGATCCGAAATTCCCGCAAACGGAACGTCCGATCTCCGCCACCCGCCTCATCCACATTCACCCGCAGCAAAGCTCACCGTACCCGCTCCGGTCAGATCTGGTCCGGTTCAAGTTCCGTTTCAAGTTCCCGCAGCAAAAAGAATCAACCATACTCAGTTCCGGTTCAAGTTCCGTTTCAAGAGGAACCCTCTTCCGTTCCCGTTCCTGTTCCTTTTTGTGGAAACGATGTAACCAAAGGACTTTCAGATTCGGAATTCAAGAATCTAATTCATGATATTTGCAATGAATATTGTGAATCAGATTCCGAAGATGACGATCTTGTTAAGGATCCTTTCAAGAAAGCAAAACGTGATGATGCTAATCTACCTTGTGCTACCACCAAGGACCTCCGTGCGAGGCGAATTTATTCCCCACTTGCCGGCGCCGGCGATTCTGATCGGGAAGTAGCTGAATTAGGGTTTTATGCTCCGTCGGTTCTGGAAAATGGCAGTGTGAGGACGGAATCAGCTGAAATTTCTGAATTAGGGTTTGGATCGCCATCAATTGCTGAAAACGGCGATACACCTGTGGATGATGCGAGGATCAAATCTAATAAGATTCTCGACGAGCCTCTGAATTTTGATAGGGGAATCACCAAGCTGTCAGATGAAGATTTGGGCGAAGAATCATTACAAACGACACCACCTAATGATGTCATCTATGTAGGTTTAGAAGCTAGCTGCGATGTGAGGAATGTGGAAGAGGCAGACACACTTGTTGTCAAAGATATGTCTCCTGCTGCTAATGTAGCTGAAAATTCAAGCGAGGGCCGGAAAAATAGCTCTGTTCAAAGGAGTAAATCTGTACTCAAACCGCGTTTACCTTTGAAATTGTTCAAAGCCCCAGGTTCATTCAATTACAAAAGGCTAATGGATGCTATGGGAGATAATTTCGGAATACCGAAATTGGGTCATTGTCATGAAGACAAGAAGGTTATGGGAGGTCAGGGATTGAAACTTCCTTTGACATCTGATAATCATGAAGGTTCTAAGCCTGAAACTAAGATTGATAGTTGCACCATGGGTGATACCAATGGGCCAGAACTGGCATCTTCACCAATGCAAGTAGATACTGAGAAAGTGAATGGCGAGTGTTTATCTGTGCCATCTGTGGATGATAAATGCTTGAGTGAATCAAAGGTGGATCTGGCTGCAGATTTTCTGGGTGCTAGAGATGTTGGTAATGCTTTCGACCACGGTGATGTTAAGCAAGATTCTTTCAACAGGAATAATCTTGACTGTGCTAAGGCTGCTGATCACAATGGCTGTGCCTTAGAGCAGCTTGGTGTTTTGAATGAAGATTGTATACCAATGACCCGTGATACTGAAATATGTGATGATATTGTGCTCAATGGAGACCAAATGCAGAGCATACCTCAGAATGTCAAACCTATGGATCTGACCAGAAACACTCAAGAAAGCACTGTTGAAGCGTTATACCCCAAAGCCCATAAGAGAAATAATCCACTCAAGAGCAAACATGTTCATAAACCATATTTGCACGGGAAGCTGATCAAAACCCCTGGTTCAGTCAACTATAGGAGGTTGCTTCCATATTTTAAGAATCTTCCCAAAGATGACTATGGTACATCTGGTGATCAGGCCCATCACAAAAATGAAGAGGCGGATTTATATGCAAAGGAGTTCCAACTTCCTTTGCCATCTCAAAGTGAAGAAGCTTCTATTAGCAGACAAATGACTGTCAGTGGCTCTATCCATGACAAAGTTGACTTGAATACTTTGGAAAACAACATCTCAGTCAATCCTGCTAATATGTCATCTCATGGCTACCAGCCAAAATTGCCATCTTTCCAAGACTCCTCCGAATTGCCAATACAATTTGACGCAAAAGAAATGGCACATGAGTGCTTATCTGCACCCTCTGTTGAGGCTAACTCTGAGAAAACTGTCATCAGTTCTAAAGATGAATGCTTGAGTGAATCAGCGACTGATCCTTGTTCCGTAATGGTGTGTGATAAGATTGTCACAAATGGTGGGTCACCACCCAAAGAACAAAATTTGCTTAATGTCAATAATGATATCTCCAACCTTTCATTTGAGCATCATTCAAGCAATGAGAAAGGATTCACTGAAAGAAAGCAGTTTGTGAGTCTGGAGAAGCATGAATCTGTTAGCAGATGTCCTCCTGAAGCTCAGAGTCTAAATCAATTGGATCCTAATATGTTAGATGCTGAAGAGAATGAAACAAGCAGCCATGCAATTTGCAAAAATGACGATGTTGTGGTTTTATGTCACGTCACCATCTCAAATGAAGAATCTAGTTCACAATCAGAGAAGATTATTTCTGAAAAACCTGCGTTACCAGACACTGCAACACATGGTGGTGGCAAAGAAAAAAGTCTTCTGAATGGCCTAGTTAATGGTTCAAAGATGCCTTCAAAAGGAAGCAATAACAAAAAGGCTAGTTTTGCTGGTGAGATGAAAAATGGTTTGGAATCCAATACTACACCGGTTCTAAATCGCTGCCCATGGGTTAAGTTTTTAAAACATGCTGGGTCTTTGAACTACAAAAGATTGCTTCCGATTTTGTTGGAAAATGAGAAGTCTAATTCCTGTGCTTCGAATAATGATCATCGCCCCAAACTTCAGAAGCTTTTGGATCACACACCATTTCTGCCAATTTCAGATTCAAACTTGCAAGTAACTCCTGGAAGTGATTCAAATGATTGTGATCCTATGGGTGATAGTACAGGAAATTCTGGTGCTCAGCAAGAAACAGAGTTGCAAGCTTGTGACTTAAATAGTGATAGTTTAAGCCCAAGATCTCAACTACAAGATAAACAGATTGTGTCAAATAGACCCTACTATCTCGAGAACTCCTTTGTTTCACCAATTTCTGTTCATAGAGATTTACCAGTCACACCTTTAGGTCCTATAGCTGATCAAGTTGTCACTAGAGAAGAAGTGGGTACACCTGCCTTGTCCAACAGGGAGAAATCACCTGAAACACCTGGATGTTGTCGGAGCTTGTCCGAAATGAAAGTCACAGATCAACTTGATGCCCTTGGCGTTAGTTCCCGAAAAGGAATTTTGAGAAAAAACCCAAGAGGATGCAGAGGGCTTTGCACATGTTTGAACTGTGTTTCTTTTCGCCTTCATGCAGAAAGAGCATTTGAATTTTCTAGAAATCAGCTGCTAGATGCCGAAGAGGTTGCCCAAGACCTTATGAAGGAAGTATCCCATCTCAGAAACATGTTGTTAGAAAGATGTACTGATAATGTCAACGACACTCCTCTTTTGGATGGAAGTCAGGTGAAAGAAGCCTGCAGGAAAGCATCTGAAACAGAACAACTTGCAAAGGATCGACTTAGCCAGATGAATGATGATCTTAACATACATTGCAGAACACCTAGTCTGCAGCGACCAAGTGTTAAGTTTTCAGATCGTGTTGAGGAAAAGGTCATTCAACCAAACAGATTAAAAAATGGTTAA |
| Protein sequence |
>Msa0577330 LDFYQKSRHKIFPPTKTLVPAITHTYINSAPIFTHFETKAAEQSRAAMDVIRNSRKRNVRSPPPASSTFTRSKAHRTRSGQIWSGSSSVSSSRSKKNQPYSVPVQVPFQEEPSSVPVPVPFCGNDVTKGLSDSEFKNLIHDICNEYCESDSEDDDLVKDPFKKAKRDDANLPCATTKDLRARRIYSPLAGAGDSDREVAELGFYAPSVLENGSVRTESAEISELGFGSPSIAENGDTPVDDARIKSNKILDEPLNFDRGITKLSDEDLGEESLQTTPPNDVIYVGLEASCDVRNVEEADTLVVKDMSPAANVAENSSEGRKNSSVQRSKSVLKPRLPLKLFKAPGSFNYKRLMDAMGDNFGIPKLGHCHEDKKVMGGQGLKLPLTSDNHEGSKPETKIDSCTMGDTNGPELASSPMQVDTEKVNGECLSVPSVDDKCLSESKVDLAADFLGARDVGNAFDHGDVKQDSFNRNNLDCAKAADHNGCALEQLGVLNEDCIPMTRDTEICDDIVLNGDQMQSIPQNVKPMDLTRNTQESTVEALYPKAHKRNNPLKSKHVHKPYLHGKLIKTPGSVNYRRLLPYFKNLPKDDYGTSGDQAHHKNEEADLYAKEFQLPLPSQSEEASISRQMTVSGSIHDKVDLNTLENNISVNPANMSSHGYQPKLPSFQDSSELPIQFDAKEMAHECLSAPSVEANSEKTVISSKDECLSESATDPCSVMVCDKIVTNGGSPPKEQNLLNVNNDISNLSFEHHSSNEKGFTERKQFVSLEKHESVSRCPPEAQSLNQLDPNMLDAEENETSSHAICKNDDVVVLCHVTISNEESSSQSEKIISEKPALPDTATHGGGKEKSLLNGLVNGSKMPSKGSNNKKASFAGEMKNGLESNTTPVLNRCPWVKFLKHAGSLNYKRLLPILLENEKSNSCASNNDHRPKLQKLLDHTPFLPISDSNLQVTPGSDSNDCDPMGDSTGNSGAQQETELQACDLNSDSLSPRSQLQDKQIVSNRPYYLENSFVSPISVHRDLPVTPLGPIADQVVTREEVGTPALSNREKSPETPGCCRSLSEMKVTDQLDALGVSSRKGILRKNPRGCRGLCTCLNCVSFRLHAERAFEFSRNQLLDAEEVAQDLMKEVSHLRNMLLERCTDNVNDTPLLDGSQVKEACRKASETEQLAKDRLSQMNDDLNIHCRTPSLQRPSVKFSDRVEEKVIQPNRLKNG* |
