AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0208110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0208110 MtrunA17_Chr2g0307011 100.000 91 0 0 1 91 287 377 1.02e-61 191
Msa0208110 MtrunA17_Chr3g0081621 38.750 80 48 1 1 80 375 453 1.03e-12 62.0
Msa0208110 MtrunA17_Chr8g0354091 38.750 80 48 1 1 80 277 355 1.24e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0208110 AT2G26830.1 60.714 84 33 0 3 86 287 370 1.39e-34 121
Msa0208110 AT4G09760.1 39.744 78 46 1 3 80 266 342 3.80e-12 60.5
Msa0208110 AT4G09760.2 39.744 78 46 1 3 80 266 342 3.80e-12 60.5

Find 41 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATATTGTTCGTTCATATT+TGG 0.100547 2_1:-42384778 None:intergenic
ACAAACACACTTCTGGAATA+TGG 0.258033 2_1:+42384414 Msa0208110:five_prime_UTR
GATATTGTTCGTTCATATTT+GGG 0.276266 2_1:-42384777 None:intergenic
GTATTGTAATCCAGAAAATT+AGG 0.282777 2_1:+42386904 Msa0208110:three_prime_UTR
AGATTTACTAATAAATAAAT+AGG 0.283666 2_1:-42384494 None:intergenic
CAAGATAATCAAAATCTATT+GGG 0.308596 2_1:-42386606 None:intergenic
CCAAGATAATCAAAATCTAT+TGG 0.314209 2_1:-42386607 None:intergenic
TCAATGAAGTAAAGTTTATC+TGG 0.320782 2_1:-42384550 None:intergenic
ACTACGATCAAAGCAAATTT+AGG 0.335363 2_1:+42386847 Msa0208110:three_prime_UTR
AACATTTAACTACATGAATC+AGG 0.346353 2_1:-42386771 None:intergenic
TGGATTACAATACAACAATA+AGG 0.362339 2_1:-42386894 None:intergenic
TAAGGAGACAAACACACTTC+TGG 0.363805 2_1:+42384407 Msa0208110:five_prime_UTR
ACGAACAATATCATTTCTTC+AGG 0.383658 2_1:+42384788 Msa0208110:CDS
ACTTCTGGAATATGGGCATT+TGG 0.401789 2_1:+42384422 Msa0208110:five_prime_UTR
TTCAGATGACATAATTTGTT+CGG 0.410215 2_1:+42387013 Msa0208110:three_prime_UTR
ATTACCTCTTGAGGTCTGTC+TGG 0.424572 2_1:-42384823 None:intergenic
CAAACACACTTCTGGAATAT+GGG 0.454061 2_1:+42384415 Msa0208110:five_prime_UTR
ATTCTCATGTCCAGAGAGAA+TGG 0.454181 2_1:+42384463 Msa0208110:five_prime_UTR
TCTACTCATTCCTTTCTAAG+AGG 0.456775 2_1:-42386699 None:intergenic
GAAAGAAAAGCTTGTATCAA+TGG 0.461918 2_1:+42386666 Msa0208110:CDS
GGTTATCAAACCATTCTCTC+TGG 0.474616 2_1:-42384473 None:intergenic
TTCGATACAACGAGTACAAA+AGG 0.487153 2_1:+42386641 Msa0208110:CDS
TAGGCCTTTAGTTTGAGCTC+TGG 0.487772 2_1:+42386866 Msa0208110:three_prime_UTR
TAGTTTATAATAAAGTCATA+AGG 0.492591 2_1:+42384389 Msa0208110:five_prime_UTR
AACTACAGAGGCTATGACAT+AGG 0.495161 2_1:+42384592 Msa0208110:five_prime_UTR
CATTCTTTCTAGATGTATTG+TGG 0.495682 2_1:+42386944 Msa0208110:three_prime_UTR
CGCTATCCTACCTCTTAGAA+AGG 0.496082 2_1:+42386689 Msa0208110:CDS
TCTTAAAACTTTGTATGTTG+AGG 0.497216 2_1:+42386429 Msa0208110:CDS
TTTCTGTTATCAAACAAACT+TGG 0.520098 2_1:-42384343 None:intergenic
GCTACCAGAGCTCAAACTAA+AGG 0.526105 2_1:-42386870 None:intergenic
AATCACTTTGCAGAATATGC+TGG 0.528361 2_1:+42384616 Msa0208110:five_prime_UTR
AGATAGTTTCTACTTCATCA+AGG 0.534604 2_1:+42386721 Msa0208110:three_prime_UTR
ACTACAGAGGCTATGACATA+GGG 0.549695 2_1:+42384593 Msa0208110:five_prime_UTR
ACAGCCAGACAGACCTCAAG+AGG 0.575109 2_1:+42384819 Msa0208110:CDS
TGAGGTAAATACATATGCAC+TGG 0.582931 2_1:+42386447 Msa0208110:CDS
ACTGTGATTATTACCTCTTG+AGG 0.585155 2_1:-42384832 None:intergenic
AAGATAATCAAAATCTATTG+GGG 0.603378 2_1:-42386605 None:intergenic
CTCATTCCTTTCTAAGAGGT+AGG 0.603691 2_1:-42386695 None:intergenic
ACATTTAACTACATGAATCA+GGG 0.626795 2_1:-42386770 None:intergenic
CAGAGACCAGAAAAGGTGTG+AGG 0.696052 2_1:-42386470 None:intergenic
TATGCATCATACAACTACAG+AGG 0.713439 2_1:+42384580 Msa0208110:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTTACTAATAAATAAAT+AGG - chr2_1:42384497-42384516 None:intergenic 10.0%
!!! TAAAGTATTTTTTTACATTT+GGG - chr2_1:42385868-42385887 None:intergenic 10.0%
!!! ATAAAGTATTTTTTTACATT+TGG - chr2_1:42385869-42385888 None:intergenic 10.0%
!! TTAAAAAAAATTATTCAATG+CGG + chr2_1:42386058-42386077 Msa0208110:intron 10.0%
!! TAGTTTATAATAAAGTCATA+AGG + chr2_1:42384389-42384408 Msa0208110:five_prime_UTR 15.0%
!! AAACTATCATTGATATTTAA+AGG + chr2_1:42385933-42385952 Msa0208110:intron 15.0%
!! TAAAAAAAATTATTCAATGC+GGG + chr2_1:42386059-42386078 Msa0208110:intron 15.0%
!!! ATATAGGTTATTTTCTTTTT+CGG + chr2_1:42386354-42386373 Msa0208110:intron 15.0%
!!! TTTAACTACTTTTTTTTATC+AGG - chr2_1:42386798-42386817 None:intergenic 15.0%
!!! TATTCAATATTTTATCATGC+AGG + chr2_1:42384752-42384771 Msa0208110:intron 20.0%
!!! TGTCTTTTTATTTTGACTTT+TGG + chr2_1:42385040-42385059 Msa0208110:intron 20.0%
!!! GTCTTTTTATTTTGACTTTT+GGG + chr2_1:42385041-42385060 Msa0208110:intron 20.0%
!! ATTAATCTCAAATTGATACT+TGG + chr2_1:42385133-42385152 Msa0208110:intron 20.0%
!!! TAATCCTATTTTTGTAGAAT+AGG + chr2_1:42385284-42385303 Msa0208110:intron 20.0%
!!! AATCCTATTTTTGTAGAATA+GGG + chr2_1:42385285-42385304 Msa0208110:intron 20.0%
!!! TAATCTTTTACGATTCAAAT+CGG - chr2_1:42385318-42385337 None:intergenic 20.0%
!! AAAAAAAATTATTCAATGCG+GGG + chr2_1:42386060-42386079 Msa0208110:intron 20.0%
!! GTACTATATAACTGTAATAT+AGG + chr2_1:42386338-42386357 Msa0208110:intron 20.0%
!! TTAATACCAATTCATAATAC+AGG + chr2_1:42386393-42386412 Msa0208110:intron 20.0%
!! TCTTATTAAAATGATATCCA+TGG + chr2_1:42386543-42386562 Msa0208110:intron 20.0%
!! TATTTCTTTGCATAATAAAC+AGG + chr2_1:42386573-42386592 Msa0208110:intron 20.0%
!! AAGATAATCAAAATCTATTG+GGG - chr2_1:42386608-42386627 None:intergenic 20.0%
!! CAAGATAATCAAAATCTATT+GGG - chr2_1:42386609-42386628 None:intergenic 20.0%
!!! GATCTTTACAAATTTTGATA+TGG + chr2_1:42386968-42386987 Msa0208110:three_prime_UTR 20.0%
! TTTCTGTTATCAAACAAACT+TGG - chr2_1:42384346-42384365 None:intergenic 25.0%
! TCAATGAAGTAAAGTTTATC+TGG - chr2_1:42384553-42384572 None:intergenic 25.0%
! GATATTGTTCGTTCATATTT+GGG - chr2_1:42384780-42384799 None:intergenic 25.0%
! TGATATTGTTCGTTCATATT+TGG - chr2_1:42384781-42384800 None:intergenic 25.0%
!!! TTTATTTTGACTTTTGGGTA+TGG + chr2_1:42385046-42385065 Msa0208110:intron 25.0%
!!! TTATTTTGACTTTTGGGTAT+GGG + chr2_1:42385047-42385066 Msa0208110:intron 25.0%
! TGATACTTGGAAAATTTACT+TGG + chr2_1:42385146-42385165 Msa0208110:intron 25.0%
!! AATCTTTTACGATTCAAATC+GGG - chr2_1:42385317-42385336 None:intergenic 25.0%
! TCATTTGGAAATTAAACAGA+AGG - chr2_1:42385428-42385447 None:intergenic 25.0%
!! AGTATTTCAAGTGTGATTTA+AGG + chr2_1:42385485-42385504 Msa0208110:intron 25.0%
!! AAGGTTTTCTTTCTTTGATT+CGG + chr2_1:42385504-42385523 Msa0208110:intron 25.0%
!!! CACACATATTTTTGAAAACT+CGG + chr2_1:42385572-42385591 Msa0208110:intron 25.0%
!!! TATTTTTTTACATTTGGGAC+GGG - chr2_1:42385863-42385882 None:intergenic 25.0%
!!! GTATTTTTTTACATTTGGGA+CGG - chr2_1:42385864-42385883 None:intergenic 25.0%
! TCTTAAAACTTTGTATGTTG+AGG + chr2_1:42386429-42386448 Msa0208110:CDS 25.0%
! CCAAGATAATCAAAATCTAT+TGG - chr2_1:42386610-42386629 None:intergenic 25.0%
!!! CCAATAGATTTTGATTATCT+TGG + chr2_1:42386607-42386626 Msa0208110:CDS 25.0%
! ACATTTAACTACATGAATCA+GGG - chr2_1:42386773-42386792 None:intergenic 25.0%
! AACATTTAACTACATGAATC+AGG - chr2_1:42386774-42386793 None:intergenic 25.0%
! TGGATTACAATACAACAATA+AGG - chr2_1:42386897-42386916 None:intergenic 25.0%
! GTATTGTAATCCAGAAAATT+AGG + chr2_1:42386904-42386923 Msa0208110:three_prime_UTR 25.0%
!!! TTCAGATGACATAATTTGTT+CGG + chr2_1:42387013-42387032 Msa0208110:three_prime_UTR 25.0%
GCAAGCATAAATAACTTACA+AGG - chr2_1:42384659-42384678 None:intergenic 30.0%
ACGAACAATATCATTTCTTC+AGG + chr2_1:42384788-42384807 Msa0208110:CDS 30.0%
AGGTAATAATCACAGTGAAA+GGG + chr2_1:42384839-42384858 Msa0208110:intron 30.0%
! ACTATATGGATCTCGTTTTA+CGG - chr2_1:42384916-42384935 None:intergenic 30.0%
TCCATATAGTCTATCAACTA+TGG + chr2_1:42384926-42384945 Msa0208110:intron 30.0%
AAGACTTTACAATTCGAATC+GGG - chr2_1:42385218-42385237 None:intergenic 30.0%
CAAGACTTTACAATTCGAAT+CGG - chr2_1:42385219-42385238 None:intergenic 30.0%
! ATCTTGCGATTTAGTGATTT+TGG + chr2_1:42385386-42385405 Msa0208110:intron 30.0%
! TTTTCCCTGATTTCAAAACA+CGG + chr2_1:42385785-42385804 Msa0208110:intron 30.0%
CAAAGAAATATCAATAGCCA+TGG - chr2_1:42386563-42386582 None:intergenic 30.0%
GAAAGAAAAGCTTGTATCAA+TGG + chr2_1:42386666-42386685 Msa0208110:CDS 30.0%
AGATAGTTTCTACTTCATCA+AGG + chr2_1:42386721-42386740 Msa0208110:three_prime_UTR 30.0%
ACTACGATCAAAGCAAATTT+AGG + chr2_1:42386847-42386866 Msa0208110:three_prime_UTR 30.0%
! GATTTGAAAGCCTAATTTTC+TGG - chr2_1:42386917-42386936 None:intergenic 30.0%
CATTCTTTCTAGATGTATTG+TGG + chr2_1:42386944-42386963 Msa0208110:three_prime_UTR 30.0%
!! ACAAACACACTTCTGGAATA+TGG + chr2_1:42384414-42384433 Msa0208110:five_prime_UTR 35.0%
!! CAAACACACTTCTGGAATAT+GGG + chr2_1:42384415-42384434 Msa0208110:five_prime_UTR 35.0%
TATGCATCATACAACTACAG+AGG + chr2_1:42384580-42384599 Msa0208110:five_prime_UTR 35.0%
AATCACTTTGCAGAATATGC+TGG + chr2_1:42384616-42384635 Msa0208110:five_prime_UTR 35.0%
!! CTTGCTTTTCTTGCTTTACT+CGG + chr2_1:42384674-42384693 Msa0208110:intron 35.0%
ACTGTGATTATTACCTCTTG+AGG - chr2_1:42384835-42384854 None:intergenic 35.0%
GAGGTAATAATCACAGTGAA+AGG + chr2_1:42384838-42384857 Msa0208110:intron 35.0%
! TTTACGGATGCTTGATAATC+TGG - chr2_1:42384900-42384919 None:intergenic 35.0%
!! GCCATAGTTGATAGACTATA+TGG - chr2_1:42384930-42384949 None:intergenic 35.0%
GACAGAAAAGATGATGATCA+TGG - chr2_1:42384952-42384971 None:intergenic 35.0%
TACAAATGCTAACACTGAAG+TGG - chr2_1:42385017-42385036 None:intergenic 35.0%
! ATTTGAGATTAATCCCTGAC+AGG - chr2_1:42385126-42385145 None:intergenic 35.0%
TGGAAAATTTACTTGGCTAG+TGG + chr2_1:42385153-42385172 Msa0208110:intron 35.0%
AAAATTTACTTGGCTAGTGG+TGG + chr2_1:42385156-42385175 Msa0208110:intron 35.0%
! TATTTTGTTACTCCTACCCA+TGG + chr2_1:42385182-42385201 Msa0208110:intron 35.0%
CCACCCTATTCTACAAAAAT+AGG - chr2_1:42385291-42385310 None:intergenic 35.0%
!!! CCTATTTTTGTAGAATAGGG+TGG + chr2_1:42385288-42385307 Msa0208110:intron 35.0%
TGTGTACTCTTAATCGATGA+AGG - chr2_1:42385361-42385380 None:intergenic 35.0%
! CATGAGAGTTTGAAGTCATT+TGG - chr2_1:42385443-42385462 None:intergenic 35.0%
TTTCCCTGATTTCAAAACAC+GGG + chr2_1:42385786-42385805 Msa0208110:intron 35.0%
!!! ATACCCGTGTTTTGAAATCA+GGG - chr2_1:42385792-42385811 None:intergenic 35.0%
TGATTTCAAAACACGGGTAT+GGG + chr2_1:42385792-42385811 Msa0208110:intron 35.0%
ATGTATTACAACGTTGCTCT+TGG + chr2_1:42386009-42386028 Msa0208110:intron 35.0%
TGTATTACAACGTTGCTCTT+GGG + chr2_1:42386010-42386029 Msa0208110:intron 35.0%
AAAATTATTCAATGCGGGGA+CGG + chr2_1:42386064-42386083 Msa0208110:intron 35.0%
ATTCAATTTCTCATCCTCGT+TGG + chr2_1:42386130-42386149 Msa0208110:intron 35.0%
TTCAATTTCTCATCCTCGTT+GGG + chr2_1:42386131-42386150 Msa0208110:intron 35.0%
ACGGGTAAGTATATGAGAAT+CGG + chr2_1:42386167-42386186 Msa0208110:intron 35.0%
AAGTATATGAGAATCGGGTA+TGG + chr2_1:42386173-42386192 Msa0208110:intron 35.0%
AGTATATGAGAATCGGGTAT+GGG + chr2_1:42386174-42386193 Msa0208110:intron 35.0%
ATAATTTACATGCCCTCAAG+TGG - chr2_1:42386314-42386333 None:intergenic 35.0%
CAGACACCTGTATTATGAAT+TGG - chr2_1:42386402-42386421 None:intergenic 35.0%
TGAGGTAAATACATATGCAC+TGG + chr2_1:42386447-42386466 Msa0208110:CDS 35.0%
! TTTTGTACTCGTTGTATCGA+AGG - chr2_1:42386642-42386661 None:intergenic 35.0%
TTCGATACAACGAGTACAAA+AGG + chr2_1:42386641-42386660 Msa0208110:CDS 35.0%
TCTACTCATTCCTTTCTAAG+AGG - chr2_1:42386702-42386721 None:intergenic 35.0%
TAAGGAGACAAACACACTTC+TGG + chr2_1:42384407-42384426 Msa0208110:five_prime_UTR 40.0%
!! ACTTCTGGAATATGGGCATT+TGG + chr2_1:42384422-42384441 Msa0208110:five_prime_UTR 40.0%
ATTCTCATGTCCAGAGAGAA+TGG + chr2_1:42384463-42384482 Msa0208110:five_prime_UTR 40.0%
GGTTATCAAACCATTCTCTC+TGG - chr2_1:42384476-42384495 None:intergenic 40.0%
AACTACAGAGGCTATGACAT+AGG + chr2_1:42384592-42384611 Msa0208110:five_prime_UTR 40.0%
ACTACAGAGGCTATGACATA+GGG + chr2_1:42384593-42384612 Msa0208110:five_prime_UTR 40.0%
TCTGTCACGCGTGTTTATTT+AGG + chr2_1:42384966-42384985 Msa0208110:intron 40.0%
!!! TTTTGACTTTTGGGTATGGG+AGG + chr2_1:42385050-42385069 Msa0208110:intron 40.0%
!! TTTGACTTTTGGGTATGGGA+GGG + chr2_1:42385051-42385070 Msa0208110:intron 40.0%
ACAAAATTCACTGACCTGTC+AGG + chr2_1:42385109-42385128 Msa0208110:intron 40.0%
CAAAATTCACTGACCTGTCA+GGG + chr2_1:42385110-42385129 Msa0208110:intron 40.0%
ATCGGGATTGTAACTACCAT+GGG - chr2_1:42385201-42385220 None:intergenic 40.0%
AATCGGGATTGTAACTACCA+TGG - chr2_1:42385202-42385221 None:intergenic 40.0%
TGTGTGCTCTTAATCGTTGA+GGG - chr2_1:42385262-42385281 None:intergenic 40.0%
TTGTGTGCTCTTAATCGTTG+AGG - chr2_1:42385263-42385282 None:intergenic 40.0%
AAAATATGTGTGCGTGTCTG+CGG - chr2_1:42385564-42385583 None:intergenic 40.0%
!! AGCTTTGCTCTGGCTTTAAA+CGG - chr2_1:42385658-42385677 None:intergenic 40.0%
AAAACCCGTCAAATTGGGTT+TGG - chr2_1:42385731-42385750 None:intergenic 40.0%
ATACCCAAACCCAGTCAAAA+CGG - chr2_1:42385756-42385775 None:intergenic 40.0%
!! TTTTGACTGGGTTTGGGTAT+GGG + chr2_1:42385756-42385775 Msa0208110:intron 40.0%
!!! CATACCCGTGTTTTGAAATC+AGG - chr2_1:42385793-42385812 None:intergenic 40.0%
CTGATTTCAAAACACGGGTA+TGG + chr2_1:42385791-42385810 Msa0208110:intron 40.0%
! TTTACATTTGGGACGGGTTT+GGG - chr2_1:42385857-42385876 None:intergenic 40.0%
!! TTTTACATTTGGGACGGGTT+TGG - chr2_1:42385858-42385877 None:intergenic 40.0%
GTATTACAACGTTGCTCTTG+GGG + chr2_1:42386011-42386030 Msa0208110:intron 40.0%
AAATTATTCAATGCGGGGAC+GGG + chr2_1:42386065-42386084 Msa0208110:intron 40.0%
CGGGTAAGTATATGAGAATC+GGG + chr2_1:42386168-42386187 Msa0208110:intron 40.0%
GTATATGAGAATCGGGTATG+GGG + chr2_1:42386175-42386194 Msa0208110:intron 40.0%
ATACAGATAGGCATGGCAAT+GGG - chr2_1:42386231-42386250 None:intergenic 40.0%
! CTCATTCCTTTCTAAGAGGT+AGG - chr2_1:42386698-42386717 None:intergenic 40.0%
ATTACCTCTTGAGGTCTGTC+TGG - chr2_1:42384826-42384845 None:intergenic 45.0%
!! TTGACTTTTGGGTATGGGAG+GGG + chr2_1:42385052-42385071 Msa0208110:intron 45.0%
GGATTGTAACTACCATGGGT+AGG - chr2_1:42385197-42385216 None:intergenic 45.0%
AAATATGTGTGCGTGTCTGC+GGG - chr2_1:42385563-42385582 None:intergenic 45.0%
! CCTCAGATTAGTCGTTCCTT+GGG - chr2_1:42385605-42385624 None:intergenic 45.0%
CCAAGGAACGACTAATCTGA+GGG + chr2_1:42385603-42385622 Msa0208110:intron 45.0%
CAAGGAACGACTAATCTGAG+GGG + chr2_1:42385604-42385623 Msa0208110:intron 45.0%
CCAATTCCACCATCCATTTG+CGG + chr2_1:42385628-42385647 Msa0208110:intron 45.0%
CAATTCCACCATCCATTTGC+GGG + chr2_1:42385629-42385648 Msa0208110:intron 45.0%
AATTCCACCATCCATTTGCG+GGG + chr2_1:42385630-42385649 Msa0208110:intron 45.0%
!! CTTTGCTCTGGCTTTAAACG+GGG - chr2_1:42385656-42385675 None:intergenic 45.0%
!! GCTTTGCTCTGGCTTTAAAC+GGG - chr2_1:42385657-42385676 None:intergenic 45.0%
CTACATACAGAGCTTTGCTC+TGG - chr2_1:42385668-42385687 None:intergenic 45.0%
CAGAGCAAAGCTCTGTATGT+AGG + chr2_1:42385666-42385685 Msa0208110:intron 45.0%
CAAAGCTCTGTATGTAGGCA+TGG + chr2_1:42385671-42385690 Msa0208110:intron 45.0%
!! AAATTGGGTTTGGTTCGGTC+GGG - chr2_1:42385721-42385740 None:intergenic 45.0%
!! CAAATTGGGTTTGGTTCGGT+CGG - chr2_1:42385722-42385741 None:intergenic 45.0%
! CCGTCAAATTGGGTTTGGTT+CGG - chr2_1:42385726-42385745 None:intergenic 45.0%
CCGAACCAAACCCAATTTGA+CGG + chr2_1:42385723-42385742 Msa0208110:intron 45.0%
CGAACCAAACCCAATTTGAC+GGG + chr2_1:42385724-42385743 Msa0208110:intron 45.0%
TACCCAAACCCAGTCAAAAC+GGG - chr2_1:42385755-42385774 None:intergenic 45.0%
!!! GTTTTGACTGGGTTTGGGTA+TGG + chr2_1:42385755-42385774 Msa0208110:intron 45.0%
TTCAAAACACGGGTATGGGA+CGG + chr2_1:42385796-42385815 Msa0208110:intron 45.0%
AATTATTCAATGCGGGGACG+GGG + chr2_1:42386066-42386085 Msa0208110:intron 45.0%
TTTCTCATCCTCGTTGGGTA+TGG + chr2_1:42386136-42386155 Msa0208110:intron 45.0%
TTCTCATCCTCGTTGGGTAT+GGG + chr2_1:42386137-42386156 Msa0208110:intron 45.0%
TACAGATAGGCATGGCAATG+GGG - chr2_1:42386230-42386249 None:intergenic 45.0%
CATACAGATAGGCATGGCAA+TGG - chr2_1:42386232-42386251 None:intergenic 45.0%
CATTGCCATGCCTATCTGTA+TGG + chr2_1:42386230-42386249 Msa0208110:intron 45.0%
!!! GTCTCAGTTTTGATTCCCAC+CGG - chr2_1:42386277-42386296 None:intergenic 45.0%
!! ACACCTTTTCTGGTCTCTGT+GGG + chr2_1:42386474-42386493 Msa0208110:CDS 45.0%
CGCTATCCTACCTCTTAGAA+AGG + chr2_1:42386689-42386708 Msa0208110:CDS 45.0%
!! TAGGCCTTTAGTTTGAGCTC+TGG + chr2_1:42386866-42386885 Msa0208110:three_prime_UTR 45.0%
GCTACCAGAGCTCAAACTAA+AGG - chr2_1:42386873-42386892 None:intergenic 45.0%
!! TGACTTTTGGGTATGGGAGG+GGG + chr2_1:42385053-42385072 Msa0208110:intron 50.0%
AATATGTGTGCGTGTCTGCG+GGG - chr2_1:42385562-42385581 None:intergenic 50.0%
AAAACTCGGTATCCAGCCCA+AGG + chr2_1:42385586-42385605 Msa0208110:intron 50.0%
! AGATTAGTCGTTCCTTGGGC+TGG - chr2_1:42385601-42385620 None:intergenic 50.0%
CCCAAGGAACGACTAATCTG+AGG + chr2_1:42385602-42385621 Msa0208110:intron 50.0%
! CCCTCAGATTAGTCGTTCCT+TGG - chr2_1:42385606-42385625 None:intergenic 50.0%
!! CCGCAAATGGATGGTGGAAT+TGG - chr2_1:42385631-42385650 None:intergenic 50.0%
CGGGGAAAACCCGTCAAATT+GGG - chr2_1:42385736-42385755 None:intergenic 50.0%
ACGGGGAAAACCCGTCAAAT+TGG - chr2_1:42385737-42385756 None:intergenic 50.0%
!!! GGGTTTTCCCCGTTTTGACT+GGG + chr2_1:42385744-42385763 Msa0208110:intron 50.0%
!!! TTCCCCGTTTTGACTGGGTT+TGG + chr2_1:42385749-42385768 Msa0208110:intron 50.0%
!!! TCCCCGTTTTGACTGGGTTT+GGG + chr2_1:42385750-42385769 Msa0208110:intron 50.0%
ACCCAAACCCAGTCAAAACG+GGG - chr2_1:42385754-42385773 None:intergenic 50.0%
ACTGGGTTTGGGTATGGGTA+TGG + chr2_1:42385761-42385780 Msa0208110:intron 50.0%
CTGGGTTTGGGTATGGGTAT+GGG + chr2_1:42385762-42385781 Msa0208110:intron 50.0%
TCAAAACACGGGTATGGGAC+GGG + chr2_1:42385797-42385816 Msa0208110:intron 50.0%
GGGACGGGTAACAGGTATAT+AGG + chr2_1:42385812-42385831 Msa0208110:intron 50.0%
! TTTGGGACGGGTTTGGGTAT+GGG - chr2_1:42385851-42385870 None:intergenic 50.0%
! ATTTGGGACGGGTTTGGGTA+TGG - chr2_1:42385852-42385871 None:intergenic 50.0%
ATCCTCGTTGGGTATGGGTA+GGG + chr2_1:42386142-42386161 Msa0208110:intron 50.0%
GTTGGGTATGGGTAGGGTAA+CGG + chr2_1:42386148-42386167 Msa0208110:intron 50.0%
TTGGGTATGGGTAGGGTAAC+GGG + chr2_1:42386149-42386168 Msa0208110:intron 50.0%
ATGAGAATCGGGTATGGGGA+CGG + chr2_1:42386179-42386198 Msa0208110:intron 50.0%
CTGGTCCATACAGATAGGCA+TGG - chr2_1:42386238-42386257 None:intergenic 50.0%
!! GTGGACTGGTCCATACAGAT+AGG - chr2_1:42386243-42386262 None:intergenic 50.0%
ATGGACCAGTCCACCGAAAT+TGG + chr2_1:42386249-42386268 Msa0208110:intron 50.0%
! ATTCCCACCGGTGCCAATTT+CGG - chr2_1:42386265-42386284 None:intergenic 50.0%
TGAATGCCCTGTTCCACTTG+AGG + chr2_1:42386298-42386317 Msa0208110:intron 50.0%
GAATGCCCTGTTCCACTTGA+GGG + chr2_1:42386299-42386318 Msa0208110:intron 50.0%
ACATGCCCTCAAGTGGAACA+GGG - chr2_1:42386307-42386326 None:intergenic 50.0%
TACATGCCCTCAAGTGGAAC+AGG - chr2_1:42386308-42386327 None:intergenic 50.0%
! CAGAGACCAGAAAAGGTGTG+AGG - chr2_1:42386473-42386492 None:intergenic 50.0%
!! CACACCTTTTCTGGTCTCTG+TGG + chr2_1:42386473-42386492 Msa0208110:CDS 50.0%
!! CACCTTTTCTGGTCTCTGTG+GGG + chr2_1:42386475-42386494 Msa0208110:CDS 50.0%
!! ACCTTTTCTGGTCTCTGTGG+GGG + chr2_1:42386476-42386495 Msa0208110:CDS 50.0%
ACAGCCAGACAGACCTCAAG+AGG + chr2_1:42384819-42384838 Msa0208110:CDS 55.0%
!! GACTTTTGGGTATGGGAGGG+GGG + chr2_1:42385054-42385073 Msa0208110:intron 55.0%
!! ACTTTTGGGTATGGGAGGGG+GGG + chr2_1:42385055-42385074 Msa0208110:intron 55.0%
ATATGTGTGCGTGTCTGCGG+GGG - chr2_1:42385561-42385580 None:intergenic 55.0%
TTTAAACGGGGCCCCGCAAA+TGG - chr2_1:42385644-42385663 None:intergenic 55.0%
GGCAACAAAACCCATACCCG+TGG + chr2_1:42385692-42385711 Msa0208110:intron 55.0%
!! TTGGTTCGGTCGGGTAACCA+CGG - chr2_1:42385712-42385731 None:intergenic 55.0%
!! CGGGTTTTCCCCGTTTTGAC+TGG + chr2_1:42385743-42385762 Msa0208110:intron 55.0%
TTTGGGTATGGGTTCGGGGT+GGG - chr2_1:42385840-42385859 None:intergenic 55.0%
ACGGGTTTGGGTATGGGTTC+GGG - chr2_1:42385845-42385864 None:intergenic 55.0%
! GACGGGTTTGGGTATGGGTT+CGG - chr2_1:42385846-42385865 None:intergenic 55.0%
TCGGGAATACCCGAACCCAT+CGG + chr2_1:42386094-42386113 Msa0208110:intron 55.0%
CATCCTCGTTGGGTATGGGT+AGG + chr2_1:42386141-42386160 Msa0208110:intron 55.0%
TACCCTACCCATACCCAACG+AGG - chr2_1:42386147-42386166 None:intergenic 55.0%
TGAGAATCGGGTATGGGGAC+GGG + chr2_1:42386180-42386199 Msa0208110:intron 55.0%
AGATAGGCATGGCAATGGGG+CGG - chr2_1:42386227-42386246 None:intergenic 55.0%
CAGTCCACCGAAATTGGCAC+CGG + chr2_1:42386255-42386274 Msa0208110:intron 55.0%
!! CACTGGCCTCACACCTTTTC+TGG + chr2_1:42386464-42386483 Msa0208110:CDS 55.0%
GTCTCTGTGGGGGCTAATTC+AGG + chr2_1:42386486-42386505 Msa0208110:CDS 55.0%
TATGTGTGCGTGTCTGCGGG+GGG - chr2_1:42385560-42385579 None:intergenic 60.0%
GGTCGGGTAACCACGGGTAT+GGG - chr2_1:42385705-42385724 None:intergenic 60.0%
!! TGGTTCGGTCGGGTAACCAC+GGG - chr2_1:42385711-42385730 None:intergenic 60.0%
ACGGGTATGGGACGGGTAAC+AGG + chr2_1:42385804-42385823 Msa0208110:intron 60.0%
GTTTGGGTATGGGTTCGGGG+TGG - chr2_1:42385841-42385860 None:intergenic 60.0%
CGGGTTTGGGTATGGGTTCG+GGG - chr2_1:42385844-42385863 None:intergenic 60.0%
ATTCAATGCGGGGACGGGGA+TGG + chr2_1:42386070-42386089 Msa0208110:intron 60.0%
TTCAATGCGGGGACGGGGAT+GGG + chr2_1:42386071-42386090 Msa0208110:intron 60.0%
CGGGAATACCCGAACCCATC+GGG + chr2_1:42386095-42386114 Msa0208110:intron 60.0%
GGGAATACCCGAACCCATCG+GGG + chr2_1:42386096-42386115 Msa0208110:intron 60.0%
ATACCCGAACCCATCGGGGA+CGG + chr2_1:42386100-42386119 Msa0208110:intron 60.0%
ATCTCATCCCCGTCCCCGAT+GGG - chr2_1:42386112-42386131 None:intergenic 60.0%
AATCTCATCCCCGTCCCCGA+TGG - chr2_1:42386113-42386132 None:intergenic 60.0%
ATAGGCATGGCAATGGGGCG+GGG - chr2_1:42386225-42386244 None:intergenic 60.0%
GATAGGCATGGCAATGGGGC+GGG - chr2_1:42386226-42386245 None:intergenic 60.0%
! CGGTGCCAATTTCGGTGGAC+TGG - chr2_1:42386257-42386276 None:intergenic 60.0%
! TCCACCGAAATTGGCACCGG+TGG + chr2_1:42386258-42386277 Msa0208110:intron 60.0%
!! CCACCGAAATTGGCACCGGT+GGG + chr2_1:42386259-42386278 Msa0208110:intron 60.0%
GCCCCCACAGAGACCAGAAA+AGG - chr2_1:42386480-42386499 None:intergenic 60.0%
AACGGGGCCCCGCAAATGGA+TGG - chr2_1:42385640-42385659 None:intergenic 65.0%
CGGTCGGGTAACCACGGGTA+TGG - chr2_1:42385706-42385725 None:intergenic 65.0%
TACCCGAACCCATCGGGGAC+GGG + chr2_1:42386101-42386120 Msa0208110:intron 65.0%
! ATCCCCGTCCCCGATGGGTT+CGG - chr2_1:42386107-42386126 None:intergenic 65.0%
! CCCACCGGTGCCAATTTCGG+TGG - chr2_1:42386262-42386281 None:intergenic 65.0%
GCGTGTCTGCGGGGGGATGT+GGG - chr2_1:42385553-42385572 None:intergenic 70.0%
TGCGTGTCTGCGGGGGGATG+TGG - chr2_1:42385554-42385573 None:intergenic 70.0%
GGGGCCCCGCAAATGGATGG+TGG - chr2_1:42385637-42385656 None:intergenic 70.0%
ATGCGGGGACGGGGATGGGT+CGG + chr2_1:42386075-42386094 Msa0208110:intron 70.0%
ACCCGAACCCATCGGGGACG+GGG + chr2_1:42386102-42386121 Msa0208110:intron 70.0%
! TCCCCGTCCCCGATGGGTTC+GGG - chr2_1:42386106-42386125 None:intergenic 70.0%
CATGGCAATGGGGCGGGGTG+GGG - chr2_1:42386220-42386239 None:intergenic 70.0%
GCATGGCAATGGGGCGGGGT+GGG - chr2_1:42386221-42386240 None:intergenic 70.0%
TGCGGGGACGGGGATGGGTC+GGG + chr2_1:42386076-42386095 Msa0208110:intron 75.0%
CAATGGGGCGGGGTGGGGAC+GGG - chr2_1:42386215-42386234 None:intergenic 75.0%
GCAATGGGGCGGGGTGGGGA+CGG - chr2_1:42386216-42386235 None:intergenic 75.0%
GGCATGGCAATGGGGCGGGG+TGG - chr2_1:42386222-42386241 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 42384332 42387041 42384332 ID=Msa0208110;Name=Msa0208110
chr2_1 mRNA 42384332 42387041 42384332 ID=Msa0208110-mRNA-1;Parent=Msa0208110;Name=Msa0208110-mRNA-1;_AED=0.00;_eAED=0.00;_QI=338|1|1|1|1|1|4|321|91
chr2_1 exon 42384332 42384659 42384332 ID=Msa0208110-mRNA-1:exon:12712;Parent=Msa0208110-mRNA-1
chr2_1 exon 42384774 42384840 42384774 ID=Msa0208110-mRNA-1:exon:12713;Parent=Msa0208110-mRNA-1
chr2_1 exon 42386415 42386507 42386415 ID=Msa0208110-mRNA-1:exon:12714;Parent=Msa0208110-mRNA-1
chr2_1 exon 42386595 42387041 42386595 ID=Msa0208110-mRNA-1:exon:12715;Parent=Msa0208110-mRNA-1
chr2_1 five_prime_UTR 42384332 42384659 42384332 ID=Msa0208110-mRNA-1:five_prime_utr;Parent=Msa0208110-mRNA-1
chr2_1 five_prime_UTR 42384774 42384783 42384774 ID=Msa0208110-mRNA-1:five_prime_utr;Parent=Msa0208110-mRNA-1
chr2_1 CDS 42384784 42384840 42384784 ID=Msa0208110-mRNA-1:cds;Parent=Msa0208110-mRNA-1
chr2_1 CDS 42386415 42386507 42386415 ID=Msa0208110-mRNA-1:cds;Parent=Msa0208110-mRNA-1
chr2_1 CDS 42386595 42386720 42386595 ID=Msa0208110-mRNA-1:cds;Parent=Msa0208110-mRNA-1
chr2_1 three_prime_UTR 42386721 42387041 42386721 ID=Msa0208110-mRNA-1:three_prime_utr;Parent=Msa0208110-mRNA-1
Gene Sequence

>Msa0208110

ATGAACGAACAATATCATTTCTTCAGGCATTACTTACAGCCAGACAGACCTCAAGAGGTGTCTGAGAATGATCTTAAAACTTTGTATGTTGAGGTAAATACATATGCACTGGCCTCACACCTTTTCTGGTCTCTGTGGGGGCTAATTCAGGCAAAGATGTCCCCAATAGATTTTGATTATCTTGGTTACTTTTTCCTTCGATACAACGAGTACAAAAGGCAGAAAGAAAAGCTTGTATCAATGGCGCTATCCTACCTCTTAGAAAGGAATGAGTAG
Protein sequence

>Msa0208110

MNEQYHFFRHYLQPDRPQEVSENDLKTLYVEVNTYALASHLFWSLWGLIQAKMSPIDFDYLGYFFLRYNEYKRQKEKLVSMALSYLLERNE*