Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0281260 | KEH37197.1 | 90.625 | 64 | 6 | 0 | 1 | 64 | 1 | 64 | 8.06e-34 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0281260 | A0A072V5T5 | 90.625 | 64 | 6 | 0 | 1 | 64 | 1 | 64 | 3.85e-34 | 120 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0012060 | Msa0281260 | 0.800569 | 1.355267e-48 | -8.615850e-47 |
| Msa0145310 | Msa0281260 | 0.804594 | 2.004490e-49 | -8.615850e-47 |
| Msa0173990 | Msa0281260 | 0.807406 | 5.134850e-50 | -8.615850e-47 |
| Msa0281260 | Msa0309550 | 0.833293 | 5.794997e-56 | -8.615850e-47 |
| Msa0281260 | Msa0309560 | 0.984829 | 1.034808e-161 | -8.615850e-47 |
| Msa0281260 | Msa0315290 | 0.804944 | 1.694145e-49 | -8.615850e-47 |
| Msa0281260 | Msa0379640 | 0.812144 | 4.915372e-51 | -8.615850e-47 |
| Msa0281260 | Msa0380740 | 0.802631 | 5.118207e-49 | -8.615850e-47 |
| Msa0281260 | Msa0466390 | 0.826289 | 2.943477e-54 | -8.615850e-47 |
| Msa0281260 | Msa0484780 | 0.806215 | 9.166810e-50 | -8.615850e-47 |
| Msa0281260 | Msa0536220 | 0.825725 | 4.007187e-54 | -8.615850e-47 |
| Msa0281260 | Msa0639820 | 0.804240 | 2.375799e-49 | -8.615850e-47 |
| Msa0281260 | Msa0669440 | 0.808992 | 2.358580e-50 | -8.615850e-47 |
| Msa0281260 | Msa0806090 | 0.809343 | 1.983382e-50 | -8.615850e-47 |
| Msa0281260 | Msa1082500 | 0.819315 | 1.239098e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0281260 | MtrunA17_Chr2g0295501 | 92.157 | 51 | 4 | 0 | 1 | 51 | 1 | 51 | 1.33e-29 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATGCAAGACCGTGTTTC+TGG | 0.222496 | 2_3:-16625003 | Msa0281260:CDS |
| CTAAGGAAGATTGGCTTAGA+AGG | 0.290742 | 2_3:-16625069 | Msa0281260:CDS |
| TGCATGCTGACCCTTCTTGT+TGG | 0.297128 | 2_3:+16625018 | None:intergenic |
| GCATGCAAGACCGTGTTTCT+GGG | 0.399891 | 2_3:-16625002 | Msa0281260:CDS |
| TCATCAGACCTAAGGAAGAT+TGG | 0.412607 | 2_3:-16625078 | Msa0281260:CDS |
| ATCAAAGTCTTCTGTAGCTA+AGG | 0.443745 | 2_3:-16624803 | None:intergenic |
| CTTCTAAGCCAATCTTCCTT+AGG | 0.445559 | 2_3:+16625070 | None:intergenic |
| GTTTCTGGGGTTAGCAAGGA+TGG | 0.473183 | 2_3:-16624988 | Msa0281260:CDS |
| TAAGGAAGATTGGCTTAGAA+GGG | 0.540665 | 2_3:-16625068 | Msa0281260:CDS |
| CCGTGTTTCTGGGGTTAGCA+AGG | 0.548213 | 2_3:-16624992 | Msa0281260:CDS |
| CACCTCCTTCATCAGACCTA+AGG | 0.563117 | 2_3:-16625086 | Msa0281260:CDS |
| TCAAAGTCTTCTGTAGCTAA+GGG | 0.567714 | 2_3:-16624802 | None:intergenic |
| CATGCAAGACCGTGTTTCTG+GGG | 0.590602 | 2_3:-16625001 | Msa0281260:CDS |
| ATCTTCCTTAGGTCTGATGA+AGG | 0.601958 | 2_3:+16625081 | None:intergenic |
| CCTTGCTAACCCCAGAAACA+CGG | 0.645121 | 2_3:+16624992 | None:intergenic |
| TTCCTTAGGTCTGATGAAGG+AGG | 0.672818 | 2_3:+16625084 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCTTTAATAGACAAGTTTTA+TGG | - | chr2_3:16624853-16624872 | Msa0281260:intron | 25.0% |
| ! | TACCATAACATGAAACTTTA+AGG | + | chr2_3:16625026-16625045 | None:intergenic | 25.0% |
| TGCCTTAAAGTTTCATGTTA+TGG | - | chr2_3:16625021-16625040 | Msa0281260:CDS | 30.0% | |
| ATATGTAGAAAAAAGCTCGA+AGG | - | chr2_3:16625051-16625070 | Msa0281260:CDS | 30.0% | |
| !! | TAAGGAAGATTGGCTTAGAA+GGG | - | chr2_3:16624827-16624846 | Msa0281260:CDS | 35.0% |
| !! | AGTTTTATGGTCCAACAAGA+AGG | - | chr2_3:16624866-16624885 | Msa0281260:intron | 35.0% |
| ! | GTTTTATGGTCCAACAAGAA+GGG | - | chr2_3:16624867-16624886 | Msa0281260:intron | 35.0% |
| ATCGAATGTAAGTTACCCTA+AGG | - | chr2_3:16624951-16624970 | Msa0281260:intron | 35.0% | |
| TCGAATGTAAGTTACCCTAA+GGG | - | chr2_3:16624952-16624971 | Msa0281260:intron | 35.0% | |
| ATCTTCCTTAGGTCTGATGA+AGG | + | chr2_3:16624817-16624836 | None:intergenic | 40.0% | |
| TCATCAGACCTAAGGAAGAT+TGG | - | chr2_3:16624817-16624836 | Msa0281260:CDS | 40.0% | |
| CTTCTAAGCCAATCTTCCTT+AGG | + | chr2_3:16624828-16624847 | None:intergenic | 40.0% | |
| !! | CTAAGGAAGATTGGCTTAGA+AGG | - | chr2_3:16624826-16624845 | Msa0281260:CDS | 40.0% |
| CGAATGTAAGTTACCCTAAG+GGG | - | chr2_3:16624953-16624972 | Msa0281260:intron | 40.0% | |
| TTCCTTAGGTCTGATGAAGG+AGG | + | chr2_3:16624814-16624833 | None:intergenic | 45.0% | |
| !! | AACGATTTTGGGTCCCCTTA+GGG | + | chr2_3:16624969-16624988 | None:intergenic | 45.0% |
| TAAGGGGACCCAAAATCGTT+GGG | - | chr2_3:16624969-16624988 | Msa0281260:CDS | 45.0% | |
| ! | GAGTCTACCCCAACGATTTT+GGG | + | chr2_3:16624980-16624999 | None:intergenic | 45.0% |
| ! | AGAGTCTACCCCAACGATTT+TGG | + | chr2_3:16624981-16625000 | None:intergenic | 45.0% |
| CACCTCCTTCATCAGACCTA+AGG | - | chr2_3:16624809-16624828 | Msa0281260:CDS | 50.0% | |
| TGCATGCTGACCCTTCTTGT+TGG | + | chr2_3:16624880-16624899 | None:intergenic | 50.0% | |
| AGCATGCAAGACCGTGTTTC+TGG | - | chr2_3:16624892-16624911 | Msa0281260:intron | 50.0% | |
| GCATGCAAGACCGTGTTTCT+GGG | - | chr2_3:16624893-16624912 | Msa0281260:intron | 50.0% | |
| ! | CATGCAAGACCGTGTTTCTG+GGG | - | chr2_3:16624894-16624913 | Msa0281260:intron | 50.0% |
| CCTTGCTAACCCCAGAAACA+CGG | + | chr2_3:16624906-16624925 | None:intergenic | 50.0% | |
| !! | GTTTCTGGGGTTAGCAAGGA+TGG | - | chr2_3:16624907-16624926 | Msa0281260:intron | 50.0% |
| !! | CAACGATTTTGGGTCCCCTT+AGG | + | chr2_3:16624970-16624989 | None:intergenic | 50.0% |
| CTAAGGGGACCCAAAATCGT+TGG | - | chr2_3:16624968-16624987 | Msa0281260:CDS | 50.0% | |
| AAGGGGACCCAAAATCGTTG+GGG | - | chr2_3:16624970-16624989 | Msa0281260:CDS | 50.0% | |
| !! | CCGTGTTTCTGGGGTTAGCA+AGG | - | chr2_3:16624903-16624922 | Msa0281260:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 16624805 | 16625112 | 16624805 | ID=Msa0281260;Name=Msa0281260 |
| chr2_3 | mRNA | 16624805 | 16625112 | 16624805 | ID=Msa0281260-mRNA-1;Parent=Msa0281260;Name=Msa0281260-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|68 |
| chr2_3 | exon | 16624960 | 16625112 | 16624960 | ID=Msa0281260-mRNA-1:exon:6843;Parent=Msa0281260-mRNA-1 |
| chr2_3 | exon | 16624805 | 16624858 | 16624805 | ID=Msa0281260-mRNA-1:exon:6842;Parent=Msa0281260-mRNA-1 |
| chr2_3 | CDS | 16624960 | 16625112 | 16624960 | ID=Msa0281260-mRNA-1:cds;Parent=Msa0281260-mRNA-1 |
| chr2_3 | CDS | 16624805 | 16624858 | 16624805 | ID=Msa0281260-mRNA-1:cds;Parent=Msa0281260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
