AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0145310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0145310 MtrunA17_Chr1g0153581 94.737 95 1 1 1 91 1 95 3.49e-61 181
Msa0145310 MtrunA17_Chr1g0153601 89.474 95 6 1 1 91 1 95 3.08e-55 166
Msa0145310 MtrunA17_Chr1g0153561 86.316 95 9 1 1 91 1 95 1.54e-54 164
Msa0145310 MtrunA17_Chr1g0153611 65.591 93 29 1 1 90 1 93 1.16e-38 124
Msa0145310 MtrunA17_Chr1g0153551 45.000 100 46 1 1 91 1 100 2.50e-19 75.5
Msa0145310 MtrunA17_Chr1g0153541 43.678 87 45 1 5 91 21 103 2.01e-16 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGATTAGTCCGTCTTTAT+TGG 0.241517 1_4:+4987195 None:intergenic
TATCTCCTTACCCGTGGTTT+TGG 0.273881 1_4:-4987145 Msa0145310:CDS
TGATCCATAGACACCCTCTT+TGG 0.332546 1_4:+4987373 None:intergenic
TATCCCAAAGAGGGTGTCTA+TGG 0.383107 1_4:-4987377 Msa0145310:CDS
GATCCATAGACACCCTCTTT+GGG 0.423736 1_4:+4987374 None:intergenic
CAACGATTGGCTCTCCATCC+AGG 0.437823 1_4:+4987235 None:intergenic
AGCTTTCATCAAACAACGAT+TGG 0.498009 1_4:+4987222 None:intergenic
TCTTGCCAAAACCACGGGTA+AGG 0.519368 1_4:+4987140 None:intergenic
CGATGGTCGTTACACAAAGA+AGG 0.521782 1_4:-4987296 Msa0145310:CDS
TAGGTTCTTACTACCCTGGA+TGG 0.528156 1_4:-4987249 Msa0145310:CDS
TTAGTTCTTGCCAAAACCAC+GGG 0.528157 1_4:+4987135 None:intergenic
TGGATCAAATCAACTTTGAA+AGG 0.536831 1_4:-4987357 Msa0145310:CDS
GATTTAGGTTCTTACTACCC+TGG 0.543944 1_4:-4987253 Msa0145310:CDS
AACGATTGGCTCTCCATCCA+GGG 0.580860 1_4:+4987236 None:intergenic
ATTAGTTCTTGCCAAAACCA+CGG 0.592102 1_4:+4987134 None:intergenic
GCCTGTGTTTATCCCAAAGA+GGG 0.602626 1_4:-4987386 Msa0145310:CDS
ATTGATTATCTCCTTACCCG+TGG 0.619921 1_4:-4987151 Msa0145310:CDS
AAAGCTGATGCCAATAAAGA+CGG 0.628000 1_4:-4987205 Msa0145310:CDS
TGCCTGTGTTTATCCCAAAG+AGG 0.630941 1_4:-4987387 Msa0145310:CDS
ACCCTCTTTGGGATAAACAC+AGG 0.645492 1_4:+4987385 None:intergenic
AGACGGACTAATCAGTGGCG+AGG 0.659853 1_4:-4987188 Msa0145310:CDS
AATAAAGACGGACTAATCAG+TGG 0.668016 1_4:-4987193 Msa0145310:CDS
AAGGCGGATATCAATAACGA+TGG 0.697636 1_4:-4987313 Msa0145310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGAACATTATAGAAAAAT+TGG - chr1_4:4987185-4987204 Msa0145310:CDS 20.0%
! CATTATAGAAAAATTGGAGA+AGG - chr1_4:4987191-4987210 Msa0145310:CDS 25.0%
!!! AAAAAAGCTCTAAAGGATTT+AGG - chr1_4:4987255-4987274 Msa0145310:CDS 25.0%
TGGATCAAATCAACTTTGAA+AGG - chr1_4:4987166-4987185 Msa0145310:CDS 30.0%
TATAGAAAAATTGGAGAAGG+CGG - chr1_4:4987194-4987213 Msa0145310:CDS 30.0%
! GGAACTCAAAAAAGCTCTAA+AGG - chr1_4:4987248-4987267 Msa0145310:CDS 35.0%
AGCTTTCATCAAACAACGAT+TGG + chr1_4:4987304-4987323 None:intergenic 35.0%
AAAGCTGATGCCAATAAAGA+CGG - chr1_4:4987318-4987337 Msa0145310:CDS 35.0%
! ACTGATTAGTCCGTCTTTAT+TGG + chr1_4:4987331-4987350 None:intergenic 35.0%
!! AATAAAGACGGACTAATCAG+TGG - chr1_4:4987330-4987349 Msa0145310:CDS 35.0%
AAGGCGGATATCAATAACGA+TGG - chr1_4:4987210-4987229 Msa0145310:CDS 40.0%
! GATTTAGGTTCTTACTACCC+TGG - chr1_4:4987270-4987289 Msa0145310:CDS 40.0%
ATTGATTATCTCCTTACCCG+TGG - chr1_4:4987372-4987391 Msa0145310:CDS 40.0%
! TTAGTTCTTGCCAAAACCAC+GGG + chr1_4:4987391-4987410 None:intergenic 40.0%
TGCCTGTGTTTATCCCAAAG+AGG - chr1_4:4987136-4987155 Msa0145310:CDS 45.0%
GCCTGTGTTTATCCCAAAGA+GGG - chr1_4:4987137-4987156 Msa0145310:CDS 45.0%
!! ACCCTCTTTGGGATAAACAC+AGG + chr1_4:4987141-4987160 None:intergenic 45.0%
!! TATCCCAAAGAGGGTGTCTA+TGG - chr1_4:4987146-4987165 Msa0145310:CDS 45.0%
GATCCATAGACACCCTCTTT+GGG + chr1_4:4987152-4987171 None:intergenic 45.0%
TGATCCATAGACACCCTCTT+TGG + chr1_4:4987153-4987172 None:intergenic 45.0%
CGATGGTCGTTACACAAAGA+AGG - chr1_4:4987227-4987246 Msa0145310:CDS 45.0%
TAGGTTCTTACTACCCTGGA+TGG - chr1_4:4987274-4987293 Msa0145310:CDS 45.0%
! TATCTCCTTACCCGTGGTTT+TGG - chr1_4:4987378-4987397 Msa0145310:CDS 45.0%
! AACGATTGGCTCTCCATCCA+GGG + chr1_4:4987290-4987309 None:intergenic 50.0%
TCTTGCCAAAACCACGGGTA+AGG + chr1_4:4987386-4987405 None:intergenic 50.0%
! CAACGATTGGCTCTCCATCC+AGG + chr1_4:4987291-4987310 None:intergenic 55.0%
!! AGACGGACTAATCAGTGGCG+AGG - chr1_4:4987335-4987354 Msa0145310:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 4987135 4987410 4987135 ID=Msa0145310;Name=Msa0145310
chr1_4 mRNA 4987135 4987410 4987135 ID=Msa0145310-mRNA-1;Parent=Msa0145310;Name=Msa0145310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|91
chr1_4 exon 4987135 4987410 4987135 ID=Msa0145310-mRNA-1:exon:2062;Parent=Msa0145310-mRNA-1
chr1_4 CDS 4987135 4987410 4987135 ID=Msa0145310-mRNA-1:cds;Parent=Msa0145310-mRNA-1
Gene Sequence

>Msa0145310

ATGCCTGTGTTTATCCCAAAGAGGGTGTCTATGGATCAAATCAACTTTGAAAGGAACATTATAGAAAAATTGGAGAAGGCGGATATCAATAACGATGGTCGTTACACAAAGAAGGAACTCAAAAAAGCTCTAAAGGATTTAGGTTCTTACTACCCTGGATGGAGAGCCAATCGTTGTTTGATGAAAGCTGATGCCAATAAAGACGGACTAATCAGTGGCGAGGAAATCGACACTTTAATTGATTATCTCCTTACCCGTGGTTTTGGCAAGAACTAA

Protein sequence

>Msa0145310

MPVFIPKRVSMDQINFERNIIEKLEKADINNDGRYTKKELKKALKDLGSYYPGWRANRCLMKADANKDGLISGEEIDTLIDYLLTRGFGKN*