AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0309550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0309550 MtrunA17_Chr2g0295501 93.182 44 3 0 1 44 1 44 3.56e-24 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGGAAGATTGGCTTAGA+AGG 0.290742 2_4:-4103115 Msa0309550:CDS
TGCATGCTGCTCCTTCTTGT+TGG 0.293203 2_4:+4103064 None:intergenic
AGCATGCAAGACCTTGCTTC+TGG 0.370001 2_4:-4103049 Msa0309550:CDS
AGGATGGAACTGTTCTTGTT+GGG 0.402264 2_4:-4103018 Msa0309550:CDS
AAGGATGGAACTGTTCTTGT+TGG 0.408271 2_4:-4103019 Msa0309550:CDS
TCATCAGACCTAAGGAAGAT+TGG 0.412607 2_4:-4103124 Msa0309550:CDS
GCTTTAATAGACAAGTTCTA+TGG 0.431998 2_4:-4103088 Msa0309550:CDS
GCATGCAAGACCTTGCTTCT+GGG 0.437774 2_4:-4103048 Msa0309550:CDS
ATCAAAGTCTTCTGTAGCTA+AGG 0.443745 2_4:-4102840 Msa0309550:CDS
CTTCTAAGCCAATCTTCCTT+AGG 0.445559 2_4:+4103116 None:intergenic
TCAAAGTCTTCTGTAGCTAA+GGG 0.531279 2_4:-4102839 Msa0309550:CDS
TAAGGAAGATTGGCTTAGAA+GGG 0.540665 2_4:-4103114 Msa0309550:CDS
GCTTCTGGGATTAGCAAGGA+TGG 0.553628 2_4:-4103034 Msa0309550:CDS
CACCTCCTTCATCAGACCTA+AGG 0.563117 2_4:-4103132 Msa0309550:CDS
AGTTCTATGGTCCAACAAGA+AGG 0.572566 2_4:-4103075 Msa0309550:CDS
GGATGGAACTGTTCTTGTTG+GGG 0.579567 2_4:-4103017 Msa0309550:intron
ATCTTCCTTAGGTCTGATGA+AGG 0.601958 2_4:+4103127 None:intergenic
CCTTGCTTCTGGGATTAGCA+AGG 0.605996 2_4:-4103038 Msa0309550:CDS
CCTTGCTAATCCCAGAAGCA+AGG 0.646637 2_4:+4103038 None:intergenic
TTCCTTAGGTCTGATGAAGG+AGG 0.672818 2_4:+4103130 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGTGCTTAAAATTTAAA+AGG + chr2_4:4103015-4103034 None:intergenic 15.0%
!!! AATTTTCCTTAAATTTAAAG+TGG - chr2_4:4103032-4103051 Msa0309550:CDS 15.0%
!!! ATTTTCCTTAAATTTAAAGT+GGG - chr2_4:4103033-4103052 Msa0309550:CDS 15.0%
GCTTTAATAGACAAGTTCTA+TGG - chr2_4:4102874-4102893 Msa0309550:CDS 30.0%
AAAATTACCCCAAAATCGTT+GGG - chr2_4:4102984-4103003 Msa0309550:intron 30.0%
TGAGACCCACTTTAAATTTA+AGG + chr2_4:4103041-4103060 None:intergenic 30.0%
ATATGTAGAAAAAAGCTCGA+AGG - chr2_4:4103082-4103101 Msa0309550:CDS 30.0%
!! TAAGGAAGATTGGCTTAGAA+GGG - chr2_4:4102848-4102867 Msa0309550:CDS 35.0%
!! TTTGGGGTAATTTTCCCTTA+GGG + chr2_4:4102978-4102997 None:intergenic 35.0%
!!! TTTTGGGGTAATTTTCCCTT+AGG + chr2_4:4102979-4102998 None:intergenic 35.0%
GAAAATTACCCCAAAATCGT+TGG - chr2_4:4102983-4103002 Msa0309550:intron 35.0%
AAATTACCCCAAAATCGTTG+GGG - chr2_4:4102985-4103004 Msa0309550:intron 35.0%
ATCAAAGTCTTCTGTAGCTA+AGG - chr2_4:4103122-4103141 Msa0309550:CDS 35.0%
TCAAAGTCTTCTGTAGCTAA+GGG - chr2_4:4103123-4103142 Msa0309550:CDS 35.0%
ATCTTCCTTAGGTCTGATGA+AGG + chr2_4:4102838-4102857 None:intergenic 40.0%
TCATCAGACCTAAGGAAGAT+TGG - chr2_4:4102838-4102857 Msa0309550:CDS 40.0%
CTTCTAAGCCAATCTTCCTT+AGG + chr2_4:4102849-4102868 None:intergenic 40.0%
!! CTAAGGAAGATTGGCTTAGA+AGG - chr2_4:4102847-4102866 Msa0309550:CDS 40.0%
AGTTCTATGGTCCAACAAGA+AGG - chr2_4:4102887-4102906 Msa0309550:intron 40.0%
AAGGATGGAACTGTTCTTGT+TGG - chr2_4:4102943-4102962 Msa0309550:intron 40.0%
AGGATGGAACTGTTCTTGTT+GGG - chr2_4:4102944-4102963 Msa0309550:intron 40.0%
TGGGTCTCAGTTTCATGTTA+TGG - chr2_4:4103052-4103071 Msa0309550:CDS 40.0%
TTCCTTAGGTCTGATGAAGG+AGG + chr2_4:4102835-4102854 None:intergenic 45.0%
GGATGGAACTGTTCTTGTTG+GGG - chr2_4:4102945-4102964 Msa0309550:intron 45.0%
TGTTGGGGTAAGTTACCCTA+AGG - chr2_4:4102960-4102979 Msa0309550:intron 45.0%
GTTGGGGTAAGTTACCCTAA+GGG - chr2_4:4102961-4102980 Msa0309550:intron 45.0%
! AGGTCTACCCCAACGATTTT+GGG + chr2_4:4102995-4103014 None:intergenic 45.0%
! AAGGTCTACCCCAACGATTT+TGG + chr2_4:4102996-4103015 None:intergenic 45.0%
CACCTCCTTCATCAGACCTA+AGG - chr2_4:4102830-4102849 Msa0309550:CDS 50.0%
TGCATGCTGCTCCTTCTTGT+TGG + chr2_4:4102901-4102920 None:intergenic 50.0%
AGCATGCAAGACCTTGCTTC+TGG - chr2_4:4102913-4102932 Msa0309550:intron 50.0%
GCATGCAAGACCTTGCTTCT+GGG - chr2_4:4102914-4102933 Msa0309550:intron 50.0%
CCTTGCTAATCCCAGAAGCA+AGG + chr2_4:4102927-4102946 None:intergenic 50.0%
!! CCTTGCTTCTGGGATTAGCA+AGG - chr2_4:4102924-4102943 Msa0309550:intron 50.0%
!! GCTTCTGGGATTAGCAAGGA+TGG - chr2_4:4102928-4102947 Msa0309550:intron 50.0%
! GGTCTACCCCAACGATTTTG+GGG + chr2_4:4102994-4103013 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 4102826 4103158 4102826 ID=Msa0309550;Name=Msa0309550
chr2_4 mRNA 4102826 4103158 4102826 ID=Msa0309550-mRNA-1;Parent=Msa0309550;Name=Msa0309550-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0|0|1|1|1|2|0|69
chr2_4 exon 4103018 4103158 4103018 ID=Msa0309550-mRNA-1:exon:1437;Parent=Msa0309550-mRNA-1
chr2_4 exon 4102826 4102894 4102826 ID=Msa0309550-mRNA-1:exon:1436;Parent=Msa0309550-mRNA-1
chr2_4 CDS 4103018 4103158 4103018 ID=Msa0309550-mRNA-1:cds;Parent=Msa0309550-mRNA-1
chr2_4 CDS 4102826 4102894 4102826 ID=Msa0309550-mRNA-1:cds;Parent=Msa0309550-mRNA-1
Gene Sequence

>Msa0309550

ATGGCACCTCCTTCATCAGACCTAAGGAAGATTGGCTTAGAAGGGTTTGCTTTAATAGACAAGTTCTATGGTCCAACAAGAAGGAGCAGCATGCAAGACCTTGCTTCTGGGATTAGCAAGGATGGAACTGTTCTTGTTGGGAAAAAAGCTCGAAGGCATGTGATCATATTTAAATCAAAGTCTTCTGTAGCTAAGGGAGAGTATATGTAG

Protein sequence

>Msa0309550

MAPPSSDLRKIGLEGFALIDKFYGPTRRSSMQDLASGISKDGTVLVGKKARRHVIIFKSKSSVAKGEYM*