Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0291600 | KEH17167.1 | 90.625 | 96 | 9 | 0 | 1 | 96 | 1 | 96 | 6.10e-35 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0291600 | A0A072TID1 | 90.625 | 96 | 9 | 0 | 1 | 96 | 1 | 96 | 2.91e-35 | 125 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023610 | Msa0291600 | 0.858306 | 9.020834e-63 | -8.615850e-47 |
| Msa0068630 | Msa0291600 | 0.821006 | 5.079258e-53 | -8.615850e-47 |
| Msa0071340 | Msa0291600 | 0.830977 | 2.166332e-55 | -8.615850e-47 |
| Msa0153200 | Msa0291600 | 0.900327 | 8.182165e-78 | -8.615850e-47 |
| Msa0162580 | Msa0291600 | 0.835231 | 1.893120e-56 | -8.615850e-47 |
| Msa0211680 | Msa0291600 | 0.908643 | 1.365370e-81 | -8.615850e-47 |
| Msa0291600 | Msa0533430 | 0.804219 | 2.399502e-49 | -8.615850e-47 |
| Msa0291600 | Msa0675510 | 0.837130 | 6.232148e-57 | -8.615850e-47 |
| Msa0291600 | Msa0818010 | 0.802268 | 6.080416e-49 | -8.615850e-47 |
| Msa0291600 | Msa0858360 | 0.800849 | 1.188362e-48 | -8.615850e-47 |
| Msa0291600 | Msa0884920 | 0.801559 | 8.505661e-49 | -8.615850e-47 |
| Msa0291600 | Msa0902020 | 0.825116 | 5.585309e-54 | -8.615850e-47 |
| Msa0291600 | Msa0912500 | 0.803433 | 3.493994e-49 | -8.615850e-47 |
| Msa0291600 | Msa1006250 | 0.806172 | 9.358939e-50 | -8.615850e-47 |
| Msa0291600 | Msa1006360 | 0.814480 | 1.507754e-51 | -8.615850e-47 |
| Msa0291600 | Msa1182730 | 0.803242 | 3.828345e-49 | -8.615850e-47 |
| Msa0291600 | Msa1187060 | 0.800622 | 1.321896e-48 | -8.615850e-47 |
| Msa0291600 | Msa1232060 | 0.805641 | 1.210095e-49 | -8.615850e-47 |
| Msa0291600 | Msa1347360 | 0.804417 | 2.181584e-49 | -8.615850e-47 |
| Msa0291600 | Msa1463140 | 0.898948 | 3.214485e-77 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTTACCAGCCATAGCTT+TGG | 0.395295 | 2_3:-40258468 | Msa0291600:CDS |
| TTCATAAGTGTTAAACGTCT+TGG | 0.397731 | 2_3:+40258641 | None:intergenic |
| GAACGAAGCACAAAAGGATA+TGG | 0.440435 | 2_3:-40258673 | Msa0291600:CDS |
| TGTTCTGTTGACATCAGTTA+AGG | 0.466515 | 2_3:+40258445 | None:intergenic |
| GATGTCAACAGAACAGTTGT+TGG | 0.472083 | 2_3:-40258437 | Msa0291600:CDS |
| AAGCACAAAAGGATATGGTT+TGG | 0.477101 | 2_3:-40258668 | Msa0291600:CDS |
| AATCGTGAACGAAGCACAAA+AGG | 0.486280 | 2_3:-40258679 | Msa0291600:CDS |
| GAGCACATCCTCAACAAGTT+GGG | 0.490646 | 2_3:-40258522 | Msa0291600:CDS |
| CACTTATGAAGATGCTCTCA+TGG | 0.494764 | 2_3:-40258628 | Msa0291600:intron |
| ATCAGTTAAGGCCAAAGCTA+TGG | 0.533572 | 2_3:+40258457 | None:intergenic |
| GGAGCACATCCTCAACAAGT+TGG | 0.535238 | 2_3:-40258523 | Msa0291600:CDS |
| AAGTTTCACAGTTGAAGTAG+TGG | 0.566854 | 2_3:-40258414 | Msa0291600:CDS |
| CTTGTTATCCCAACTTGTTG+AGG | 0.571320 | 2_3:+40258514 | None:intergenic |
| TTTCACAGTTGAAGTAGTGG+TGG | 0.573034 | 2_3:-40258411 | Msa0291600:CDS |
| GTTAAGGCCAAAGCTATGGC+TGG | 0.581908 | 2_3:+40258461 | None:intergenic |
| AGTAGAAGAATATGATGCCA+TGG | 0.617826 | 2_3:+40258719 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCAAGAATCTTTCTAAAAA+TGG | - | chr2_3:40258499-40258518 | Msa0291600:CDS | 20.0% |
| !!! | TCAAGAATCTTTCTAAAAAT+GGG | - | chr2_3:40258500-40258519 | Msa0291600:CDS | 20.0% |
| !! | GTATGATTTTCTATCTACTA+TGG | - | chr2_3:40258522-40258541 | Msa0291600:CDS | 25.0% |
| ! | TTCACGATTGAAACATTTTC+AGG | + | chr2_3:40258391-40258410 | None:intergenic | 30.0% |
| TTCATAAGTGTTAAACGTCT+TGG | + | chr2_3:40258443-40258462 | None:intergenic | 30.0% | |
| ! | CTATCTACTATGGTTTTTCA+AGG | - | chr2_3:40258532-40258551 | Msa0291600:intron | 30.0% |
| AAGCACAAAAGGATATGGTT+TGG | - | chr2_3:40258413-40258432 | Msa0291600:CDS | 35.0% | |
| !!! | TACTATGGTTTTTCAAGGCT+AGG | - | chr2_3:40258537-40258556 | Msa0291600:intron | 35.0% |
| TGTTCTGTTGACATCAGTTA+AGG | + | chr2_3:40258639-40258658 | None:intergenic | 35.0% | |
| AAGTTTCACAGTTGAAGTAG+TGG | - | chr2_3:40258667-40258686 | Msa0291600:CDS | 35.0% | |
| AATCGTGAACGAAGCACAAA+AGG | - | chr2_3:40258402-40258421 | Msa0291600:CDS | 40.0% | |
| GAACGAAGCACAAAAGGATA+TGG | - | chr2_3:40258408-40258427 | Msa0291600:CDS | 40.0% | |
| CACTTATGAAGATGCTCTCA+TGG | - | chr2_3:40258453-40258472 | Msa0291600:CDS | 40.0% | |
| !! | CTTGTTATCCCAACTTGTTG+AGG | + | chr2_3:40258570-40258589 | None:intergenic | 40.0% |
| TTTCTTACCAGCCATAGCTT+TGG | - | chr2_3:40258613-40258632 | Msa0291600:intron | 40.0% | |
| ATCAGTTAAGGCCAAAGCTA+TGG | + | chr2_3:40258627-40258646 | None:intergenic | 40.0% | |
| GATGTCAACAGAACAGTTGT+TGG | - | chr2_3:40258644-40258663 | Msa0291600:CDS | 40.0% | |
| TTTCACAGTTGAAGTAGTGG+TGG | - | chr2_3:40258670-40258689 | Msa0291600:CDS | 40.0% | |
| GAGCACATCCTCAACAAGTT+GGG | - | chr2_3:40258559-40258578 | Msa0291600:intron | 45.0% | |
| GGAGCACATCCTCAACAAGT+TGG | - | chr2_3:40258558-40258577 | Msa0291600:intron | 50.0% | |
| GTTAAGGCCAAAGCTATGGC+TGG | + | chr2_3:40258623-40258642 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 40258364 | 40258739 | 40258364 | ID=Msa0291600;Name=Msa0291600 |
| chr2_3 | mRNA | 40258364 | 40258739 | 40258364 | ID=Msa0291600-mRNA-1;Parent=Msa0291600;Name=Msa0291600-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|1|1|1|2|0|98 |
| chr2_3 | exon | 40258629 | 40258739 | 40258629 | ID=Msa0291600-mRNA-1:exon:12931;Parent=Msa0291600-mRNA-1 |
| chr2_3 | exon | 40258364 | 40258549 | 40258364 | ID=Msa0291600-mRNA-1:exon:12930;Parent=Msa0291600-mRNA-1 |
| chr2_3 | CDS | 40258629 | 40258739 | 40258629 | ID=Msa0291600-mRNA-1:cds;Parent=Msa0291600-mRNA-1 |
| chr2_3 | CDS | 40258364 | 40258549 | 40258364 | ID=Msa0291600-mRNA-1:cds;Parent=Msa0291600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
