Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1182730 | KEH18048.1 | 74.324 | 74 | 18 | 1 | 1 | 73 | 1 | 74 | 6.34e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1182730 | sp|P93331|NOD1_MEDTR | 44.595 | 74 | 39 | 2 | 1 | 73 | 1 | 73 | 4.42e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023610 | Msa1182730 | 0.826803 | 2.220155e-54 | -8.615850e-47 |
| Msa0071340 | Msa1182730 | 0.811699 | 6.143652e-51 | -8.615850e-47 |
| Msa0627410 | Msa1182730 | 0.803534 | 3.329197e-49 | -8.615850e-47 |
| Msa0675510 | Msa1182730 | 0.818999 | 1.462296e-52 | -8.615850e-47 |
| Msa0153200 | Msa1182730 | 0.851585 | 8.064881e-61 | -8.615850e-47 |
| Msa0162580 | Msa1182730 | 0.814261 | 1.685556e-51 | -8.615850e-47 |
| Msa0211680 | Msa1182730 | 0.819258 | 1.276723e-52 | -8.615850e-47 |
| Msa0456200 | Msa1182730 | 0.804534 | 2.062994e-49 | -8.615850e-47 |
| Msa0999340 | Msa1182730 | 0.837921 | 3.907996e-57 | -8.615850e-47 |
| Msa0999390 | Msa1182730 | 0.814637 | 1.391901e-51 | -8.615850e-47 |
| Msa1006250 | Msa1182730 | 0.836939 | 6.975005e-57 | -8.615850e-47 |
| Msa0291600 | Msa1182730 | 0.803242 | 3.828345e-49 | -8.615850e-47 |
| Msa0353690 | Msa1182730 | 0.810179 | 1.311185e-50 | -8.615850e-47 |
| Msa1131820 | Msa1182730 | 0.800789 | 1.222175e-48 | -8.615850e-47 |
| Msa1182730 | Msa1288000 | 0.810054 | 1.394797e-50 | -8.615850e-47 |
| Msa1182730 | Msa1310530 | 0.941394 | 4.489278e-101 | -8.615850e-47 |
| Msa1182730 | Msa1347360 | 0.988213 | 3.806852e-173 | -8.615850e-47 |
| Msa1182730 | Msa1359820 | 0.838400 | 2.941216e-57 | -8.615850e-47 |
| Msa1182730 | Msa1463140 | 0.835052 | 2.100281e-56 | -8.615850e-47 |
| Msa0533430 | Msa1182730 | 0.805973 | 1.030736e-49 | -8.615850e-47 |
| Msa0884920 | Msa1182730 | 0.839713 | 1.344034e-57 | -8.615850e-47 |
| Msa0912500 | Msa1182730 | 0.809607 | 1.740553e-50 | -8.615850e-47 |
| Msa0965750 | Msa1182730 | 0.802879 | 4.549471e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1182730 | MtrunA17_Chr8g0337731 | 74.324 | 74 | 18 | 1 | 1 | 73 | 1 | 74 | 5.83e-33 | 108 |
| Msa1182730 | MtrunA17_Chr8g0337721 | 71.622 | 74 | 20 | 1 | 1 | 73 | 1 | 74 | 3.28e-30 | 101 |
| Msa1182730 | MtrunA17_Chr8g0337781 | 54.795 | 73 | 33 | 0 | 1 | 73 | 1 | 73 | 9.72e-24 | 85.1 |
| Msa1182730 | MtrunA17_Chr8g0337741 | 59.722 | 72 | 28 | 1 | 3 | 73 | 1 | 72 | 1.25e-22 | 82.0 |
| Msa1182730 | MtrunA17_Chr8g0337711 | 49.315 | 73 | 37 | 0 | 1 | 73 | 1 | 73 | 2.33e-20 | 76.6 |
| Msa1182730 | MtrunA17_Chr8g0337061 | 40.278 | 72 | 42 | 1 | 1 | 71 | 9 | 80 | 3.24e-13 | 58.5 |
| Msa1182730 | MtrunA17_Chr6g0458787 | 44.595 | 74 | 39 | 2 | 1 | 73 | 1 | 73 | 4.14e-13 | 58.2 |
| Msa1182730 | MtrunA17_Chr6g0458744 | 44.595 | 74 | 39 | 2 | 1 | 73 | 37 | 109 | 1.21e-12 | 57.8 |
| Msa1182730 | MtrunA17_Chr6g0458644 | 44.595 | 74 | 39 | 2 | 1 | 73 | 37 | 109 | 1.21e-12 | 57.8 |
| Msa1182730 | MtrunA17_Chr6g0458701 | 44.595 | 74 | 39 | 2 | 1 | 73 | 37 | 109 | 1.58e-12 | 57.4 |
| Msa1182730 | MtrunA17_Chr6g0458807 | 41.892 | 74 | 41 | 2 | 1 | 73 | 1 | 73 | 1.59e-12 | 56.6 |
| Msa1182730 | MtrunA17_Chr8g0337751 | 46.575 | 73 | 32 | 2 | 1 | 73 | 1 | 66 | 1.31e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAGATGAAAACACTTCT+TGG | 0.303033 | 8_1:+17909184 | Msa1182730:CDS |
| TGTGACTTTGTTTGCAAGAA+TGG | 0.416156 | 8_1:+17909745 | Msa1182730:CDS |
| GTGACTTTGTTTGCAAGAAT+GGG | 0.440082 | 8_1:+17909746 | Msa1182730:CDS |
| CACACACTTCACTTAGAGTT+GGG | 0.463643 | 8_1:-17909726 | None:intergenic |
| TTCTTTGCTAGTTCATGCGT+TGG | 0.502913 | 8_1:+17909703 | Msa1182730:CDS |
| TCACACACTTCACTTAGAGT+TGG | 0.517794 | 8_1:-17909727 | None:intergenic |
| AAGAATGGGGACAAATTACC+AGG | 0.567345 | 8_1:+17909760 | Msa1182730:CDS |
| GAAGCTGTGGTGCATATTGA+AGG | 0.581749 | 8_1:+17909661 | Msa1182730:CDS |
| GAGCAGCTAAGAGAATAAAG+AGG | 0.606301 | 8_1:-17909219 | None:intergenic |
| TGACTTTGTTTGCAAGAATG+GGG | 0.653532 | 8_1:+17909747 | Msa1182730:CDS |
| GTAAAATGCAGAAGAAGCTG+TGG | 0.696941 | 8_1:+17909648 | Msa1182730:intron |
| AATGGGGACAAATTACCAGG+TGG | 0.759307 | 8_1:+17909763 | Msa1182730:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATATGCTTTTTTTGAACA+AGG | + | chr8_1:17909248-17909267 | Msa1182730:intron | 20.0% |
| !!! | TAAGCAAATTTTCATCTTTA+AGG | + | chr8_1:17909353-17909372 | Msa1182730:intron | 20.0% |
| !!! | TAAGCAAATTTTCATCTTTA+AGG | - | chr8_1:17909353-17909372 | None:intergenic | 20.0% |
| !! | AATTTGCTTATAAATGAGAC+CGG | - | chr8_1:17909443-17909462 | None:intergenic | 25.0% |
| !! | AATTTGCTTATAAATGAGAC+CGG | + | chr8_1:17909443-17909462 | Msa1182730:intron | 25.0% |
| !!! | TTTTTTTGAACAAGGCTTGT+AGG | + | chr8_1:17909256-17909275 | Msa1182730:intron | 30.0% |
| ! | GAACTTTAGAAGAGAAAAGA+AGG | - | chr8_1:17909305-17909324 | None:intergenic | 30.0% |
| CATTGAATAAACGATGTTTG+TGG | + | chr8_1:17909378-17909397 | Msa1182730:intron | 30.0% | |
| ATGGAGATGAAAACACTTCT+TGG | + | chr8_1:17909184-17909203 | Msa1182730:CDS | 35.0% | |
| ! | TGTGACTTTGTTTGCAAGAA+TGG | + | chr8_1:17909745-17909764 | Msa1182730:CDS | 35.0% |
| ! | GTGACTTTGTTTGCAAGAAT+GGG | + | chr8_1:17909746-17909765 | Msa1182730:CDS | 35.0% |
| ! | TGACTTTGTTTGCAAGAATG+GGG | + | chr8_1:17909747-17909766 | Msa1182730:CDS | 35.0% |
| GAGCAGCTAAGAGAATAAAG+AGG | - | chr8_1:17909222-17909241 | None:intergenic | 40.0% | |
| TTGCTTATAAATGAGACCGG+AGG | - | chr8_1:17909446-17909465 | None:intergenic | 40.0% | |
| CATTGAATGAACGATGTCTG+TGG | - | chr8_1:17909408-17909427 | None:intergenic | 40.0% | |
| TTGCTTATAAATGAGACCGG+AGG | + | chr8_1:17909446-17909465 | Msa1182730:intron | 40.0% | |
| TGCTTATAAATGAGACCGGA+GGG | + | chr8_1:17909447-17909466 | Msa1182730:intron | 40.0% | |
| AATCAGAATCATATCCCCTC+CGG | - | chr8_1:17909465-17909484 | None:intergenic | 40.0% | |
| GTAAAATGCAGAAGAAGCTG+TGG | + | chr8_1:17909648-17909667 | Msa1182730:intron | 40.0% | |
| TTCTTTGCTAGTTCATGCGT+TGG | + | chr8_1:17909703-17909722 | Msa1182730:CDS | 40.0% | |
| CACACACTTCACTTAGAGTT+GGG | - | chr8_1:17909729-17909748 | None:intergenic | 40.0% | |
| TCACACACTTCACTTAGAGT+TGG | - | chr8_1:17909730-17909749 | None:intergenic | 40.0% | |
| AAGAATGGGGACAAATTACC+AGG | + | chr8_1:17909760-17909779 | Msa1182730:CDS | 40.0% | |
| GTTCTTTCGAGATACTCCTC+CGG | + | chr8_1:17909321-17909340 | Msa1182730:intron | 45.0% | |
| GCTTATAAATGAGACCGGAG+GGG | + | chr8_1:17909448-17909467 | Msa1182730:intron | 45.0% | |
| GAAGCTGTGGTGCATATTGA+AGG | + | chr8_1:17909661-17909680 | Msa1182730:CDS | 45.0% | |
| AATGGGGACAAATTACCAGG+TGG | + | chr8_1:17909763-17909782 | Msa1182730:CDS | 45.0% | |
| !! | TCATTTTTGCAGGAACCACC+TGG | - | chr8_1:17909781-17909800 | None:intergenic | 45.0% |
| AGGTGGTTCCTGCAAAAATG+AGG | + | chr8_1:17909780-17909799 | Msa1182730:CDS | 45.0% | |
| !! | CATGCACTCCTCATTTTTGC+AGG | - | chr8_1:17909791-17909810 | None:intergenic | 45.0% |
| !!! | TAATATTTTCAATTATTAAA+AGG | - | chr8_1:17909511-17909530 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 17909184 | 17909822 | 17909184 | ID=Msa1182730;Name=Msa1182730 |
| chr8_1 | mRNA | 17909184 | 17909822 | 17909184 | ID=Msa1182730-mRNA-1;Parent=Msa1182730;Name=Msa1182730-mRNA-1;_AED=0.51;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|73 |
| chr8_1 | exon | 17909184 | 17909241 | 17909184 | ID=Msa1182730-mRNA-1:exon:6781;Parent=Msa1182730-mRNA-1 |
| chr8_1 | exon | 17909659 | 17909822 | 17909659 | ID=Msa1182730-mRNA-1:exon:6782;Parent=Msa1182730-mRNA-1 |
| chr8_1 | CDS | 17909184 | 17909241 | 17909184 | ID=Msa1182730-mRNA-1:cds;Parent=Msa1182730-mRNA-1 |
| chr8_1 | CDS | 17909659 | 17909822 | 17909659 | ID=Msa1182730-mRNA-1:cds;Parent=Msa1182730-mRNA-1 |
| Gene Sequence |
| Protein sequence |
