Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1369830 | XP_003608913.2 | 99.147 | 703 | 6 | 0 | 1 | 703 | 1 | 703 | 0.0 | 1422 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1369830 | sp|Q94BR1|MRF1_ARATH | 75.356 | 702 | 169 | 4 | 1 | 700 | 1 | 700 | 0.0 | 1065 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026780 | Msa1369830 | 0.801960 | 7.034468e-49 | -8.615850e-47 |
| Msa0029700 | Msa1369830 | 0.839739 | 1.323142e-57 | -8.615850e-47 |
| Msa0080020 | Msa1369830 | 0.844806 | 6.017923e-59 | -8.615850e-47 |
| Msa0110240 | Msa1369830 | 0.802891 | 4.524874e-49 | -8.615850e-47 |
| Msa0124960 | Msa1369830 | 0.844552 | 7.046593e-59 | -8.615850e-47 |
| Msa0154730 | Msa1369830 | 0.842463 | 2.546116e-58 | -8.615850e-47 |
| Msa0307380 | Msa1369830 | 0.814999 | 1.157150e-51 | -8.615850e-47 |
| Msa0311900 | Msa1369830 | 0.800733 | 1.255044e-48 | -8.615850e-47 |
| Msa0316140 | Msa1369830 | 0.837436 | 5.205233e-57 | -8.615850e-47 |
| Msa0372100 | Msa1369830 | 0.801968 | 7.009360e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1369830 | MtrunA17_Chr4g0058021 | 99.147 | 703 | 6 | 0 | 1 | 703 | 1 | 703 | 0.0 | 1422 |
| Msa1369830 | MtrunA17_Chr6g0465321 | 56.752 | 622 | 255 | 5 | 86 | 696 | 22 | 640 | 0.0 | 687 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1369830 | AT5G63190.2 | 75.356 | 702 | 169 | 4 | 1 | 700 | 1 | 700 | 0.0 | 1065 |
| Msa1369830 | AT5G63190.1 | 75.356 | 702 | 169 | 4 | 1 | 700 | 1 | 700 | 0.0 | 1065 |
| Msa1369830 | AT4G24800.4 | 74.856 | 696 | 171 | 3 | 5 | 699 | 2 | 694 | 0.0 | 1025 |
| Msa1369830 | AT4G24800.1 | 74.856 | 696 | 171 | 3 | 5 | 699 | 2 | 694 | 0.0 | 1025 |
| Msa1369830 | AT4G24800.3 | 74.856 | 696 | 171 | 3 | 5 | 699 | 2 | 694 | 0.0 | 1025 |
| Msa1369830 | AT4G24800.2 | 74.856 | 696 | 171 | 3 | 5 | 699 | 2 | 694 | 0.0 | 1025 |
| Msa1369830 | AT3G48390.1 | 72.442 | 606 | 162 | 2 | 96 | 700 | 30 | 631 | 0.0 | 904 |
| Msa1369830 | AT1G22730.1 | 50.156 | 642 | 284 | 9 | 78 | 698 | 51 | 677 | 0.0 | 603 |
Find 249 sgRNAs with CRISPR-Local
Find 387 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCCTTTCACCATTTGGC+TGG | 0.441435 | tig0018912:-132634 | None:intergenic |
| AAACGGTGGTGTGAGAGAAA+CGG | 0.568099 | tig0018912:-129537 | None:intergenic |
| AAAGAACAGAGAAAAGGAAA+TGG | 0.507361 | tig0018912:+132971 | Msa1369830:CDS |
| AAAGTGATGAATCGGTGGAA+AGG | 0.668878 | tig0018912:-129485 | None:intergenic |
| AAAGTTAGGGCGGGCTAAGA+AGG | 0.432147 | tig0018912:+130215 | Msa1369830:CDS |
| AAATCCATCCCTAATCTGTG+TGG | 0.607645 | tig0018912:-132142 | None:intergenic |
| AAATGCTGAAGATACAACGC+TGG | 0.642106 | tig0018912:+133073 | Msa1369830:CDS |
| AACAGTAAAGGTTCTGCTGA+AGG | 0.520154 | tig0018912:-132570 | None:intergenic |
| AACTACTGTTACTGACCCCT+TGG | 0.468009 | tig0018912:+131906 | Msa1369830:CDS |
| AAGAATATCGAAACCCTAAA+CGG | 0.517598 | tig0018912:-129554 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAATATCGAAACCCTAAA+CGG | - | tig0018912:129557-129576 | None:intergenic | 30.0% | |
| AGATGAATAGGTGATAGATT+AGG | + | tig0018912:130406-130425 | Msa1369830:intron | 30.0% | |
| CTAAAAAGTGGTATTGTATC+TGG | - | tig0018912:130780-130799 | None:intergenic | 30.0% | |
| AGCTAATCTTATTGATCATG+AGG | + | tig0018912:131148-131167 | Msa1369830:intron | 30.0% | |
| CATGCGATAATAATCTACAT+TGG | + | tig0018912:131425-131444 | Msa1369830:intron | 30.0% | |
| GTACAGAAGAGTTAACAATA+TGG | - | tig0018912:131762-131781 | None:intergenic | 30.0% | |
| ACAACAAGAGAAAGATAGAA+TGG | - | tig0018912:131806-131825 | None:intergenic | 30.0% | |
| AGATGAATACTTCAGTAATG+GGG | + | tig0018912:131960-131979 | Msa1369830:CDS | 30.0% | |
| TTTAACAGCTTCAACAGTAA+AGG | - | tig0018912:132585-132604 | None:intergenic | 30.0% | |
| AAAGAACAGAGAAAAGGAAA+TGG | + | tig0018912:132971-132990 | Msa1369830:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018912 | gene | 129479 | 134076 | 129479 | ID=Msa1369830;Name=Msa1369830 |
| tig0018912 | mRNA | 129479 | 134076 | 129479 | ID=Msa1369830-mRNA-1;Parent=Msa1369830;Name=Msa1369830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=248|1|1|1|0.66|0.5|4|397|703 |
| tig0018912 | exon | 129479 | 129695 | 129479 | ID=Msa1369830-mRNA-1:exon:1351;Parent=Msa1369830-mRNA-1 |
| tig0018912 | exon | 129984 | 130237 | 129984 | ID=Msa1369830-mRNA-1:exon:1352;Parent=Msa1369830-mRNA-1 |
| tig0018912 | exon | 131465 | 131559 | 131465 | ID=Msa1369830-mRNA-1:exon:1353;Parent=Msa1369830-mRNA-1 |
| tig0018912 | exon | 131886 | 134076 | 131886 | ID=Msa1369830-mRNA-1:exon:1354;Parent=Msa1369830-mRNA-1 |
| tig0018912 | five_prime_UTR | 129479 | 129695 | 129479 | ID=Msa1369830-mRNA-1:five_prime_utr;Parent=Msa1369830-mRNA-1 |
| tig0018912 | five_prime_UTR | 129984 | 130014 | 129984 | ID=Msa1369830-mRNA-1:five_prime_utr;Parent=Msa1369830-mRNA-1 |
| tig0018912 | CDS | 130015 | 130237 | 130015 | ID=Msa1369830-mRNA-1:cds;Parent=Msa1369830-mRNA-1 |
| tig0018912 | CDS | 131465 | 131559 | 131465 | ID=Msa1369830-mRNA-1:cds;Parent=Msa1369830-mRNA-1 |
| tig0018912 | CDS | 131886 | 133679 | 131886 | ID=Msa1369830-mRNA-1:cds;Parent=Msa1369830-mRNA-1 |
| tig0018912 | three_prime_UTR | 133680 | 134076 | 133680 | ID=Msa1369830-mRNA-1:three_prime_utr;Parent=Msa1369830-mRNA-1 |
| Gene Sequence |
>Msa1369830 ATGGCGTCGAACGAGGGATTTTTGACTGAAGGACAGAGGGAGATGTTGAAAATTGCGAGTCAGAACGCTGAGAATTTGTCGTCGTCGCCTAAGTCGCCGTCGACATTGCTTGCTGATCATCATCACATCAAAGCTCCTGCTGGTGGTAAGGCACAGACTGCTGGGATTGCTGTGAGGCATGTGCGTCGGTCGCATTCTGGAAAGTTAGGGCGGGCTAAGAAGGATGGTGCTGGTGGTAAGGGCACTTGGGGGAAATTGCTGGACACAGAAATTGATTCTCACATAGACCGCAATGATCCAAATTATGATAGTGGCGAGGAACCCTATGAGCTGGTTGGAACTACTGTTACTGACCCCTTGGATGAATTCAAGAAAGCAGTGGTATCCCTCATAGATGAATACTTCAGTAATGGGGATGTTGATTTGGCAGCATCTGACCTAAGAGAACTTGGCTCAAGTGAATATTATCCATACTTCATTAAGCGACTTGTTTCCATGGCAATGGACAGGCATGATAAGGAGAAAGAAATGGCTTCTGTTCTGCTTTCAGCACTGTATGCTGATGTCATTAGTCCCACACAGATTAGGGATGGATTTTTTATGCTTATTGAATCTGCTGATGATCTTGCAGTGGATATCCTGGATGCAGTTGACATCCTTGCTTTATTCCTGGCACGTGCTGTTGTTGATGATATTCTTCCACCAGCCTTTCTTGCCAGGGCAAGGAAGGCTCTTCCGGAATCTTCCAAGGGAGCTGAGGTAGTCAAGACTGCCGAGAAGAGCTATCTCTCAGCTCCACACCATGCAGAACTTGTGGAAAGACGATGGGGTGGTAGCACTCACATTACTGTTGAAGAAATGAAGAAAAAGATTGCTGATTTACTTAAAGAATATGTTGATAGTGGCGAGACACTTGAAGCCTGTAGGTGTATACGTGAGTTGGGAGTTGCATTCTTCCATCACGAGGTTGTGAAGAAGGCCCTGGTACTTGCCATGGAGATTCCTTCAGCAGAACCTTTACTGTTGAAGCTGTTAAAAGAAGCAGCAGCAGAAGGACTGATTAGTTCCAGCCAAATGGTGAAAGGGTTTTCTCGATTGGAAGAAGGCCTAGATGATCTTGCTCTTGATATTCCATCAGCTAAAGCCTTGTTTCAGTCATTTGTCCCCAAGGCAATATCTGAAGGATGGCTTGATGCTTCCTTTGCCAATCCAGCTGGTGAAAATGGGGAATTCCAAGTTGAAGATGAGAATGTGAGGAAGTACAAAAAGGAAGCTGTAACTATAATTCATGAGTATTTTCTCTCTGACGACATTCCTGAACTCATTCGAAGCCTAGAAGATCTTGGAGCACCTGAGTATAACCCAATATTTTTGAAGAGGTTAATAACACTTGCTTTGGACCGAAAGAACAGAGAAAAGGAAATGGCATCTGTCCTGCTATCTGCACTTCATATAGAGATATTCTCAACAGAGGATATTGTTAATGGTTTTGTCATGCTTCTGGAAAATGCTGAAGATACAACGCTGGATATACTGGATGCTTCGAATGAGCTTGCTCTGTTTTTAGCTCGGGCTGTGATTGATGATGTACTTGCCCCATTGAACTTGGATGAAATTGGCAGCAGACTACCACCAAAATGCAGTGGTAGTGAAACTGTGCGTATGGCTCGAACACTCTCTTCTGCTCGTCATGCAGGTGAAAGGCTATTGAGATGCTGGGGCGGGGGAACTGGATGGGCTGTGGAGGATGCTAAGGACAAGATCACAAAGCTCTTGGAGGAGTATGAAAGTGGGGGGGTTGTCGGTGAAGCATGCCAGTGTATTCGTGACTTGGGGATGCCTTTCTTCAACCATGAGGTAGTGAAGAAAGCTTTGGTTATGGCCATGGAGAAGAAGAATGATAGGATGCTGGATTTGCTGCAGGAGTGCTTCAGTGAAGGCCTCATCACCACCAATCAGCTGACTAAAGGATTCACCCGGATTAAGGAGGGTCTTGATGATCTGGCTCTGGACATTCCAAACGCCAAGGAAAAATTTGCTTTCTATGTGGAGCATGCACAGACCAAGGGGTGGCTTCTACCATCATTTGATTCCTCTGCCCCAGATGTCTAG |
| Protein sequence |
>Msa1369830 MASNEGFLTEGQREMLKIASQNAENLSSSPKSPSTLLADHHHIKAPAGGKAQTAGIAVRHVRRSHSGKLGRAKKDGAGGKGTWGKLLDTEIDSHIDRNDPNYDSGEEPYELVGTTVTDPLDEFKKAVVSLIDEYFSNGDVDLAASDLRELGSSEYYPYFIKRLVSMAMDRHDKEKEMASVLLSALYADVISPTQIRDGFFMLIESADDLAVDILDAVDILALFLARAVVDDILPPAFLARARKALPESSKGAEVVKTAEKSYLSAPHHAELVERRWGGSTHITVEEMKKKIADLLKEYVDSGETLEACRCIRELGVAFFHHEVVKKALVLAMEIPSAEPLLLKLLKEAAAEGLISSSQMVKGFSRLEEGLDDLALDIPSAKALFQSFVPKAISEGWLDASFANPAGENGEFQVEDENVRKYKKEAVTIIHEYFLSDDIPELIRSLEDLGAPEYNPIFLKRLITLALDRKNREKEMASVLLSALHIEIFSTEDIVNGFVMLLENAEDTTLDILDASNELALFLARAVIDDVLAPLNLDEIGSRLPPKCSGSETVRMARTLSSARHAGERLLRCWGGGTGWAVEDAKDKITKLLEEYESGGVVGEACQCIRDLGMPFFNHEVVKKALVMAMEKKNDRMLDLLQECFSEGLITTNQLTKGFTRIKEGLDDLALDIPNAKEKFAFYVEHAQTKGWLLPSFDSSAPDV* |
