AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0337740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337740 MtrunA17_Chr2g0325021 90.763 249 6 1 1 232 1 249 2.60e-160 443
Msa0337740 MtrunA17_Chr7g0263981 39.086 197 101 3 16 193 17 213 6.20e-37 130
Msa0337740 MtrunA17_Chr4g0029331 45.556 180 76 4 14 171 33 212 8.47e-37 130
Msa0337740 MtrunA17_Chr4g0029501 34.222 225 121 5 31 228 66 290 1.27e-30 114
Msa0337740 MtrunA17_Chr4g0029441 36.181 199 100 3 31 210 66 256 6.59e-29 110
Msa0337740 MtrunA17_Chr2g0326431 43.919 148 66 2 38 168 41 188 1.69e-28 108
Msa0337740 MtrunA17_Chr4g0026611 38.418 177 87 4 31 185 70 246 4.14e-27 105
Msa0337740 MtrunA17_Chr2g0284431 34.831 178 96 2 38 197 89 264 1.49e-25 101
Msa0337740 MtrunA17_Chr2g0326421 32.287 223 126 5 29 232 60 276 2.36e-25 100
Msa0337740 MtrunA17_Chr2g0284411 34.831 178 96 2 38 197 68 243 2.58e-25 100
Msa0337740 MtrunA17_Chr8g0379621 32.549 255 131 10 14 231 66 316 1.39e-24 99.0
Msa0337740 MtrunA17_Chr8g0379631 32.157 255 132 10 14 231 66 316 2.76e-24 98.2
Msa0337740 MtrunA17_Chr8g0379611 32.579 221 126 4 31 230 70 288 7.90e-23 94.4
Msa0337740 MtrunA17_Chr4g0029431 33.742 163 90 1 27 171 56 218 8.58e-22 90.9
Msa0337740 MtrunA17_Chr2g0284451 31.674 221 112 7 20 203 50 268 1.51e-21 90.5
Msa0337740 MtrunA17_Chr7g0246041 31.933 238 124 7 12 215 161 394 1.57e-21 92.0
Msa0337740 MtrunA17_Chr2g0326411 34.211 152 82 1 38 171 69 220 2.33e-21 90.1
Msa0337740 MtrunA17_Chr5g0448001 29.762 168 99 3 37 186 47 213 1.43e-18 82.0
Msa0337740 MtrunA17_Chr5g0448211 25.417 240 140 5 26 231 185 419 8.40e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337740 AT2G20520.1 46.122 245 100 6 12 232 9 245 1.83e-59 188
Msa0337740 AT1G03870.1 47.907 215 88 4 37 232 38 247 2.21e-56 180
Msa0337740 AT5G44130.1 50.655 229 88 5 6 213 3 227 5.15e-54 174
Msa0337740 AT5G03170.1 49.451 182 72 3 25 187 23 203 9.43e-49 160
Msa0337740 AT5G60490.1 40.940 149 70 2 38 168 39 187 9.10e-30 111
Msa0337740 AT2G04780.1 30.729 192 108 5 37 207 45 232 1.39e-16 76.6
Msa0337740 AT2G04780.2 30.729 192 108 5 37 207 45 232 1.39e-16 76.6
Msa0337740 AT3G60900.1 30.851 188 115 5 18 192 171 356 2.71e-16 77.4
Msa0337740 AT4G12730.1 32.195 205 115 8 26 212 179 377 1.16e-12 67.0
Msa0337740 AT2G45470.1 31.844 179 105 5 30 193 180 356 1.53e-11 63.5

Find 64 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCCTGAGGGTGCTGGTGC+TGG 0.134067 2_4:-57806717 None:intergenic
AGGGTGCTGGTGCTGGGGCT+TGG 0.151988 2_4:-57806710 None:intergenic
AAGATTGCTGTGATGTTTAT+TGG 0.222736 2_4:-57806738 None:intergenic
GGCCTGAGGGTGCTGGTGCT+GGG 0.229466 2_4:-57806716 None:intergenic
CTTCTGGTAAAGAAGGTAAT+TGG 0.246525 2_4:+57807189 Msa0337740:CDS
GGGTGCTGGTGCTGGGGCTT+GGG 0.247233 2_4:-57806709 None:intergenic
CCAGAAGCTTGTGTTCTAAC+TGG 0.262827 2_4:-57807173 None:intergenic
TCATTTGATCTTCTTTGTCT+TGG 0.265276 2_4:+57807580 Msa0337740:three_prime_UTR
CATTTGATCTTCTTTGTCTT+GGG 0.284575 2_4:+57807581 Msa0337740:three_prime_UTR
TCGGAAAGAGAGTAGTATTT+TGG 0.290230 2_4:-57807131 None:intergenic
CTGGTGCTGGGGCTTGGGCA+TGG 0.308800 2_4:-57806704 None:intergenic
GCTGGAAGGAATGTTGGTTT+TGG 0.343086 2_4:+57807479 Msa0337740:CDS
TCCTTTGGCTGTTTATCAAT+TGG 0.345240 2_4:+57807301 Msa0337740:CDS
TTTGGATTTGTTGCTGGGCT+TGG 0.356493 2_4:+57807497 Msa0337740:CDS
TTCTGGTAAAGAAGGTAATT+GGG 0.362073 2_4:+57807190 Msa0337740:CDS
GGGGCTTGGGCATGGATTTG+TGG 0.365915 2_4:-57806696 None:intergenic
AGATTGCTGTGATGTTTATT+GGG 0.382866 2_4:-57806737 None:intergenic
AACATTGACTTGGTTACCTT+GGG 0.391497 2_4:-57807228 None:intergenic
GTTTGAAACTGTTAAGAAAT+CGG 0.392584 2_4:-57807150 None:intergenic
GTGAGAAGAATGAGTGCAAC+AGG 0.394196 2_4:-57806654 None:intergenic
TTTGAGACAACAATTTCCTT+TGG 0.394518 2_4:+57807286 Msa0337740:CDS
TGTCTCAAAGCATTGTTGAT+AGG 0.394899 2_4:-57807272 None:intergenic
AACCAAGTCAATGTTTCAAC+TGG 0.415400 2_4:+57807236 Msa0337740:CDS
CTTCAGAAGTTGAGTTGAGT+TGG 0.431445 2_4:-57806833 None:intergenic
AAGTTGAGTTGAGTTGGGTT+TGG 0.439465 2_4:-57806827 None:intergenic
TTTATTGGGCCTGAGGGTGC+TGG 0.445785 2_4:-57806723 None:intergenic
GCCTGAGGGTGCTGGTGCTG+GGG 0.448279 2_4:-57806715 None:intergenic
ATGGATTTGTGGAGTTAAGA+AGG 0.451732 2_4:-57806685 None:intergenic
TTCAGAAGTTGAGTTGAGTT+GGG 0.456224 2_4:-57806832 None:intergenic
TGGGCTTGGTTTGATTTGCA+TGG 0.457325 2_4:+57807511 Msa0337740:CDS
CCAGTTAGAACACAAGCTTC+TGG 0.459667 2_4:+57807173 Msa0337740:CDS
CAGAAGCTTGTGTTCTAACT+GGG 0.462249 2_4:-57807172 None:intergenic
GCCCCAGCACCAGCACCCTC+AGG 0.463369 2_4:+57806714 Msa0337740:CDS
AAGAAGGATGATAGTGCAGC+TGG 0.464254 2_4:+57807461 Msa0337740:CDS
GGGCTTGGTTTGATTTGCAT+GGG 0.469150 2_4:+57807512 Msa0337740:CDS
CCGAGCAGCAGCGACGTTGA+AGG 0.469244 2_4:+57807413 Msa0337740:CDS
AAACATTGACTTGGTTACCT+TGG 0.472673 2_4:-57807229 None:intergenic
CTCCAGTTGAAACATTGACT+TGG 0.506376 2_4:-57807238 None:intergenic
GAATGAGTTGAACTTTCTGT+TGG 0.508496 2_4:-57807094 None:intergenic
GGTTTGATTTGATATGAACC+TGG 0.511093 2_4:+57807602 Msa0337740:three_prime_UTR
ACACAAGCTTCTGGTAAAGA+AGG 0.515910 2_4:+57807182 Msa0337740:CDS
ATCTCCAGCGTCTAGTAAGA+AGG 0.521844 2_4:+57807445 Msa0337740:CDS
CCTTCAACGTCGCTGCTGCT+CGG 0.523660 2_4:-57807413 None:intergenic
TTCTTACTAGACGCTGGAGA+TGG 0.526597 2_4:-57807443 None:intergenic
CAACTCAACTCAACTTCTGA+AGG 0.527769 2_4:+57806834 Msa0337740:CDS
TAAGTCTCCATCTTCATCTC+CGG 0.540687 2_4:+57807358 Msa0337740:CDS
TTTCATTGAGGAGACGAATG+AGG 0.550194 2_4:-57806785 None:intergenic
TGGATTTGTGGAGTTAAGAA+GGG 0.551170 2_4:-57806684 None:intergenic
TTAGGAGCCGGAGATGAAGA+TGG 0.552898 2_4:-57807365 None:intergenic
ATAAATACAAAGAATAAGCC+AGG 0.554395 2_4:-57807620 None:intergenic
GTGATGTTTATTGGGCCTGA+GGG 0.556035 2_4:-57806729 None:intergenic
GGTCTTCACTTCAATTCCCA+AGG 0.569295 2_4:+57807212 Msa0337740:CDS
TCCAATTGATAAACAGCCAA+AGG 0.570662 2_4:-57807302 None:intergenic
GGCTGTTTATCAATTGGACA+AGG 0.577680 2_4:+57807307 Msa0337740:CDS
ATTGAGGAGACGAATGAGGA+AGG 0.579677 2_4:-57806781 None:intergenic
GAACTTTCTGTTGGTCTGAG+AGG 0.589738 2_4:-57807085 None:intergenic
AGTGCAGCTGGAAGGAATGT+TGG 0.593131 2_4:+57807473 Msa0337740:CDS
TGTGATGTTTATTGGGCCTG+AGG 0.596385 2_4:-57806730 None:intergenic
TCATCCTTCTTACTAGACGC+TGG 0.596871 2_4:-57807449 None:intergenic
AGTATAAATAAGTTTCACAT+TGG 0.607356 2_4:+57807718 Msa0337740:three_prime_UTR
TCTGGTAAAGAAGGTAATTG+GGG 0.623440 2_4:+57807191 Msa0337740:CDS
AGGATGATAGTGCAGCTGGA+AGG 0.628901 2_4:+57807465 Msa0337740:CDS
TGAGTTGTTGAGTTTCATTG+AGG 0.637415 2_4:-57806797 None:intergenic
GAGTGCAACAGGTGTTGTAG+TGG 0.647059 2_4:-57806643 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATATATAATACCTGA+TGG - chr2_4:57806903-57806922 None:intergenic 15.0%
!! TTTATATATATAATACCTGA+TGG - chr2_4:57806903-57806922 None:intergenic 15.0%
!! TACATGCAAGTTATAATTAT+AGG - chr2_4:57807015-57807034 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG + chr2_4:57807050-57807069 Msa0337740:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG + chr2_4:57807718-57807737 Msa0337740:three_prime_UTR 20.0%
!! TACATGCAAGTTATAATTAT+AGG - chr2_4:57807015-57807034 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG + chr2_4:57807050-57807069 Msa0337740:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG + chr2_4:57807718-57807737 Msa0337740:three_prime_UTR 20.0%
!! GTTTGAAACTGTTAAGAAAT+CGG - chr2_4:57807153-57807172 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG - chr2_4:57807623-57807642 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG + chr2_4:57807673-57807692 Msa0337740:three_prime_UTR 25.0%
!! GTTTGAAACTGTTAAGAAAT+CGG - chr2_4:57807153-57807172 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG - chr2_4:57807623-57807642 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG + chr2_4:57807673-57807692 Msa0337740:three_prime_UTR 25.0%
AGATTGCTGTGATGTTTATT+GGG - chr2_4:57806740-57806759 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG - chr2_4:57806741-57806760 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG - chr2_4:57806893-57806912 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG - chr2_4:57807124-57807143 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG + chr2_4:57807190-57807209 Msa0337740:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG + chr2_4:57807286-57807305 Msa0337740:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG + chr2_4:57807332-57807351 Msa0337740:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG + chr2_4:57807580-57807599 Msa0337740:three_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG + chr2_4:57807581-57807600 Msa0337740:three_prime_UTR 30.0%
AGATTGCTGTGATGTTTATT+GGG - chr2_4:57806740-57806759 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG - chr2_4:57806741-57806760 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG - chr2_4:57806893-57806912 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG - chr2_4:57807124-57807143 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG + chr2_4:57807190-57807209 Msa0337740:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG + chr2_4:57807286-57807305 Msa0337740:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG + chr2_4:57807332-57807351 Msa0337740:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG + chr2_4:57807580-57807599 Msa0337740:three_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG + chr2_4:57807581-57807600 Msa0337740:three_prime_UTR 30.0%
TGGATTTGTGGAGTTAAGAA+GGG - chr2_4:57806687-57806706 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG - chr2_4:57806688-57806707 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG + chr2_4:57806750-57806769 Msa0337740:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG - chr2_4:57806800-57806819 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG - chr2_4:57806835-57806854 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG - chr2_4:57807097-57807116 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG - chr2_4:57807134-57807153 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG + chr2_4:57807189-57807208 Msa0337740:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG + chr2_4:57807191-57807210 Msa0337740:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG - chr2_4:57807231-57807250 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG - chr2_4:57807232-57807251 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG + chr2_4:57807236-57807255 Msa0337740:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG - chr2_4:57807275-57807294 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG + chr2_4:57807301-57807320 Msa0337740:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG - chr2_4:57807305-57807324 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG - chr2_4:57807341-57807360 None:intergenic 35.0%
!!! TTGGTTTTGGATTTGTTGCT+GGG + chr2_4:57807492-57807511 Msa0337740:CDS 35.0%
!! GGTTTGATTTGATATGAACC+TGG + chr2_4:57807602-57807621 Msa0337740:three_prime_UTR 35.0%
TGGATTTGTGGAGTTAAGAA+GGG - chr2_4:57806687-57806706 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG - chr2_4:57806688-57806707 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG + chr2_4:57806750-57806769 Msa0337740:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG - chr2_4:57806800-57806819 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG - chr2_4:57806835-57806854 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG - chr2_4:57807097-57807116 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG - chr2_4:57807134-57807153 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG + chr2_4:57807189-57807208 Msa0337740:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG + chr2_4:57807191-57807210 Msa0337740:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG - chr2_4:57807231-57807250 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG - chr2_4:57807232-57807251 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG + chr2_4:57807236-57807255 Msa0337740:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG - chr2_4:57807275-57807294 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG + chr2_4:57807301-57807320 Msa0337740:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG - chr2_4:57807305-57807324 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG - chr2_4:57807341-57807360 None:intergenic 35.0%
!!! TTGGTTTTGGATTTGTTGCT+GGG + chr2_4:57807492-57807511 Msa0337740:CDS 35.0%
!! GGTTTGATTTGATATGAACC+TGG + chr2_4:57807602-57807621 Msa0337740:three_prime_UTR 35.0%
TTTCATTGAGGAGACGAATG+AGG - chr2_4:57806788-57806807 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG - chr2_4:57806830-57806849 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG - chr2_4:57806836-57806855 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG + chr2_4:57806834-57806853 Msa0337740:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG - chr2_4:57806875-57806894 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG - chr2_4:57806876-57806895 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG - chr2_4:57806889-57806908 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG - chr2_4:57807175-57807194 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG + chr2_4:57807182-57807201 Msa0337740:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG - chr2_4:57807241-57807260 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG + chr2_4:57807307-57807326 Msa0337740:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG + chr2_4:57807358-57807377 Msa0337740:CDS 40.0%
!!! GTTGGTTTTGGATTTGTTGC+TGG + chr2_4:57807491-57807510 Msa0337740:CDS 40.0%
TTTCATTGAGGAGACGAATG+AGG - chr2_4:57806788-57806807 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG - chr2_4:57806830-57806849 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG - chr2_4:57806836-57806855 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG + chr2_4:57806834-57806853 Msa0337740:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG - chr2_4:57806875-57806894 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG - chr2_4:57806876-57806895 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG - chr2_4:57806889-57806908 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG - chr2_4:57807175-57807194 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG + chr2_4:57807182-57807201 Msa0337740:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG - chr2_4:57807241-57807260 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG + chr2_4:57807307-57807326 Msa0337740:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG + chr2_4:57807358-57807377 Msa0337740:CDS 40.0%
!!! GTTGGTTTTGGATTTGTTGC+TGG + chr2_4:57807491-57807510 Msa0337740:CDS 40.0%
GTGAGAAGAATGAGTGCAAC+AGG - chr2_4:57806657-57806676 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG - chr2_4:57806732-57806751 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG - chr2_4:57806733-57806752 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG - chr2_4:57806784-57806803 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG - chr2_4:57806874-57806893 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG + chr2_4:57806885-57806904 Msa0337740:intron 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG - chr2_4:57807088-57807107 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG - chr2_4:57807176-57807195 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG + chr2_4:57807173-57807192 Msa0337740:CDS 45.0%
GGTCTTCACTTCAATTCCCA+AGG + chr2_4:57807212-57807231 Msa0337740:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG - chr2_4:57807386-57807405 None:intergenic 45.0%
TTCTTACTAGACGCTGGAGA+TGG - chr2_4:57807446-57807465 None:intergenic 45.0%
ATCTCCAGCGTCTAGTAAGA+AGG + chr2_4:57807445-57807464 Msa0337740:CDS 45.0%
TCATCCTTCTTACTAGACGC+TGG - chr2_4:57807452-57807471 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG + chr2_4:57807461-57807480 Msa0337740:CDS 45.0%
!!! GCTGGAAGGAATGTTGGTTT+TGG + chr2_4:57807479-57807498 Msa0337740:CDS 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG + chr2_4:57807497-57807516 Msa0337740:CDS 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG + chr2_4:57807511-57807530 Msa0337740:CDS 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG + chr2_4:57807512-57807531 Msa0337740:CDS 45.0%
GTGAGAAGAATGAGTGCAAC+AGG - chr2_4:57806657-57806676 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG - chr2_4:57806732-57806751 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG - chr2_4:57806733-57806752 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG - chr2_4:57806784-57806803 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG - chr2_4:57806874-57806893 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG + chr2_4:57806885-57806904 Msa0337740:intron 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG - chr2_4:57807088-57807107 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG - chr2_4:57807176-57807195 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG + chr2_4:57807173-57807192 Msa0337740:CDS 45.0%
GGTCTTCACTTCAATTCCCA+AGG + chr2_4:57807212-57807231 Msa0337740:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG - chr2_4:57807386-57807405 None:intergenic 45.0%
TTCTTACTAGACGCTGGAGA+TGG - chr2_4:57807446-57807465 None:intergenic 45.0%
ATCTCCAGCGTCTAGTAAGA+AGG + chr2_4:57807445-57807464 Msa0337740:CDS 45.0%
TCATCCTTCTTACTAGACGC+TGG - chr2_4:57807452-57807471 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG + chr2_4:57807461-57807480 Msa0337740:CDS 45.0%
!!! GCTGGAAGGAATGTTGGTTT+TGG + chr2_4:57807479-57807498 Msa0337740:CDS 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG + chr2_4:57807497-57807516 Msa0337740:CDS 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG + chr2_4:57807511-57807530 Msa0337740:CDS 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG + chr2_4:57807512-57807531 Msa0337740:CDS 45.0%
!!! ATAGTAATTTTTTTTATTAA+AGG - chr2_4:57807744-57807763 None:intergenic 5.0%
!!! ATAGTAATTTTTTTTATTAA+AGG - chr2_4:57807744-57807763 None:intergenic 5.0%
! GAGTGCAACAGGTGTTGTAG+TGG - chr2_4:57806646-57806665 None:intergenic 50.0%
TTAGGAGCCGGAGATGAAGA+TGG - chr2_4:57807368-57807387 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG - chr2_4:57807380-57807399 None:intergenic 50.0%
!!! CTGCTGCTCGGAGTTTTAGA+AGG - chr2_4:57807404-57807423 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG + chr2_4:57807465-57807484 Msa0337740:CDS 50.0%
AGTGCAGCTGGAAGGAATGT+TGG + chr2_4:57807473-57807492 Msa0337740:CDS 50.0%
! GAGTGCAACAGGTGTTGTAG+TGG - chr2_4:57806646-57806665 None:intergenic 50.0%
TTAGGAGCCGGAGATGAAGA+TGG - chr2_4:57807368-57807387 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG - chr2_4:57807380-57807399 None:intergenic 50.0%
!!! CTGCTGCTCGGAGTTTTAGA+AGG - chr2_4:57807404-57807423 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG + chr2_4:57807465-57807484 Msa0337740:CDS 50.0%
AGTGCAGCTGGAAGGAATGT+TGG + chr2_4:57807473-57807492 Msa0337740:CDS 50.0%
! TTTATTGGGCCTGAGGGTGC+TGG - chr2_4:57806726-57806745 None:intergenic 55.0%
! TTTATTGGGCCTGAGGGTGC+TGG - chr2_4:57806726-57806745 None:intergenic 55.0%
GGGGCTTGGGCATGGATTTG+TGG - chr2_4:57806699-57806718 None:intergenic 60.0%
CCTTCAACGTCGCTGCTGCT+CGG - chr2_4:57807416-57807435 None:intergenic 60.0%
GGGGCTTGGGCATGGATTTG+TGG - chr2_4:57806699-57806718 None:intergenic 60.0%
CCTTCAACGTCGCTGCTGCT+CGG - chr2_4:57807416-57807435 None:intergenic 60.0%
CCGAGCAGCAGCGACGTTGA+AGG + chr2_4:57807413-57807432 Msa0337740:CDS 65.0%
CCGAGCAGCAGCGACGTTGA+AGG + chr2_4:57807413-57807432 Msa0337740:CDS 65.0%
! CTGGTGCTGGGGCTTGGGCA+TGG - chr2_4:57806707-57806726 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG - chr2_4:57806712-57806731 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG - chr2_4:57806713-57806732 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG - chr2_4:57806718-57806737 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG - chr2_4:57806719-57806738 None:intergenic 70.0%
! CTGGTGCTGGGGCTTGGGCA+TGG - chr2_4:57806707-57806726 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG - chr2_4:57806712-57806731 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG - chr2_4:57806713-57806732 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG - chr2_4:57806718-57806737 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG - chr2_4:57806719-57806738 None:intergenic 70.0%
GCCCCAGCACCAGCACCCTC+AGG + chr2_4:57806714-57806733 Msa0337740:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG - chr2_4:57806720-57806739 None:intergenic 75.0%
GCCCCAGCACCAGCACCCTC+AGG + chr2_4:57806714-57806733 Msa0337740:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG - chr2_4:57806720-57806739 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 57806633 57807771 57806633 ID=Msa0337740;Name=Msa0337740
chr2_4 mRNA 57806633 57807771 57806633 ID=Msa0337740-mRNA-1;Parent=Msa0337740;Name=Msa0337740-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0.5|1|1|1|2|224|232
chr2_4 exon 57806633 57806855 57806633 ID=Msa0337740-mRNA-1:exon:17638;Parent=Msa0337740-mRNA-1
chr2_4 exon 57807072 57807771 57807072 ID=Msa0337740-mRNA-1:exon:17639;Parent=Msa0337740-mRNA-1
chr2_4 CDS 57806633 57806855 57806633 ID=Msa0337740-mRNA-1:cds;Parent=Msa0337740-mRNA-1
chr2_4 CDS 57807072 57807547 57807072 ID=Msa0337740-mRNA-1:cds;Parent=Msa0337740-mRNA-1
chr2_4 three_prime_UTR 57807548 57807771 57807548 ID=Msa0337740-mRNA-1:three_prime_utr;Parent=Msa0337740-mRNA-1
Gene Sequence

>Msa0337740

ATGGCACTCACCACTACAACACCTGTTGCACTCATTCTTCTCACTCTCATTCCCTTCTTAACTCCACAAATCCATGCCCAAGCCCCAGCACCAGCACCCTCAGGCCCAATAAACATCACAGCAATCTTTGAAAAAGCTGGTCAATACACCTTCCTCATTCGTCTCCTCAATGAAACTCAACAACTCACTCAAATCCAAACCCAACTCAACTCAACTTCTGAAGGAGCAATCAACGACCTCTCAGACCAACAGAAAGTTCAACTCATTCTCTACCATGTTACACCAAAATACTACTCTCTTTCCGATTTCTTAACAGTTTCAAACCCAGTTAGAACACAAGCTTCTGGTAAAGAAGGTAATTGGGGTCTTCACTTCAATTCCCAAGGTAACCAAGTCAATGTTTCAACTGGAGTTGTTACTGTACCTATCAACAATGCTTTGAGACAACAATTTCCTTTGGCTGTTTATCAATTGGACAAGGTTTTGTTACCTTCTGAACTTTTTGGTGCTAAGTCTCCATCTTCATCTCCGGCTCCTAAGTCGTCGAAAACTCCTTCTAAAACTCCGAGCAGCAGCGACGTTGAAGGTGATGCTCCATCTCCAGCGTCTAGTAAGAAGGATGATAGTGCAGCTGGAAGGAATGTTGGTTTTGGATTTGTTGCTGGGCTTGGTTTGATTTGCATGGGAGTTCTTTTTTGA
Protein sequence

>Msa0337740

MALTTTTPVALILLTLIPFLTPQIHAQAPAPAPSGPINITAIFEKAGQYTFLIRLLNETQQLTQIQTQLNSTSEGAINDLSDQQKVQLILYHVTPKYYSLSDFLTVSNPVRTQASGKEGNWGLHFNSQGNQVNVSTGVVTVPINNALRQQFPLAVYQLDKVLLPSELFGAKSPSSSPAPKSSKTPSKTPSSSDVEGDAPSPASSKKDDSAAGRNVGFGFVAGLGLICMGVLF*