AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0220960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220960 MtrunA17_Chr2g0325021 97.590 249 5 1 1 248 1 249 2.96e-176 484
Msa0220960 MtrunA17_Chr7g0263981 50.289 173 84 2 16 186 17 189 1.29e-51 168
Msa0220960 MtrunA17_Chr4g0029331 49.444 180 86 2 14 188 33 212 5.18e-48 159
Msa0220960 MtrunA17_Chr4g0029501 36.889 225 131 3 31 244 66 290 8.46e-41 141
Msa0220960 MtrunA17_Chr2g0326431 48.649 148 76 0 38 185 41 188 3.79e-40 139
Msa0220960 MtrunA17_Chr4g0029441 40.000 190 108 2 31 214 66 255 1.49e-38 135
Msa0220960 MtrunA17_Chr4g0026611 43.229 192 99 5 27 211 63 251 2.21e-38 135
Msa0220960 MtrunA17_Chr8g0379621 37.402 254 136 10 14 247 66 316 1.26e-35 129
Msa0220960 MtrunA17_Chr8g0379631 37.008 254 137 10 14 247 66 316 2.83e-35 127
Msa0220960 MtrunA17_Chr2g0326421 35.294 221 138 4 29 248 60 276 1.32e-33 122
Msa0220960 MtrunA17_Chr2g0284411 40.449 178 102 3 38 213 68 243 5.19e-33 121
Msa0220960 MtrunA17_Chr2g0284431 40.449 178 102 3 38 213 89 264 5.70e-33 121
Msa0220960 MtrunA17_Chr2g0284451 36.818 220 118 6 20 219 50 268 3.75e-32 119
Msa0220960 MtrunA17_Chr8g0379611 35.000 220 138 4 31 246 70 288 1.67e-31 118
Msa0220960 MtrunA17_Chr4g0029431 38.650 163 99 1 27 188 56 218 2.58e-30 114
Msa0220960 MtrunA17_Chr2g0326411 40.789 152 89 1 38 188 69 220 2.97e-30 114
Msa0220960 MtrunA17_Chr7g0246041 37.198 207 115 6 12 218 161 352 6.97e-29 112
Msa0220960 MtrunA17_Chr5g0448001 35.057 174 107 3 37 209 47 215 1.01e-28 109
Msa0220960 MtrunA17_Chr5g0448211 30.612 245 137 8 26 247 185 419 8.65e-19 84.7
Msa0220960 MtrunA17_Chr2g0296571 28.755 233 151 7 8 234 164 387 3.69e-14 71.2
Msa0220960 MtrunA17_Chr3g0102421 28.105 153 96 4 38 185 193 336 5.47e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220960 AT2G20520.1 52.869 244 101 5 12 248 9 245 1.05e-73 225
Msa0220960 AT1G03870.1 56.279 215 86 5 37 248 38 247 7.50e-72 220
Msa0220960 AT5G44130.1 56.889 225 92 3 6 226 3 226 7.32e-70 215
Msa0220960 AT5G03170.1 56.593 182 76 3 25 204 23 203 2.15e-61 193
Msa0220960 AT5G60490.1 47.682 151 78 1 36 185 37 187 1.51e-41 142
Msa0220960 AT2G04780.1 34.896 192 116 5 37 223 45 232 4.41e-24 97.4
Msa0220960 AT2G04780.2 34.896 192 116 5 37 223 45 232 4.41e-24 97.4
Msa0220960 AT3G60900.1 33.000 200 123 4 18 217 171 359 1.26e-23 99.0
Msa0220960 AT3G46550.1 29.487 234 152 6 20 248 190 415 2.21e-18 84.0
Msa0220960 AT2G45470.1 32.278 158 102 2 30 186 180 333 2.68e-18 83.6
Msa0220960 AT4G12730.1 37.349 166 92 7 25 187 178 334 2.55e-17 80.9
Msa0220960 AT5G55730.2 31.169 154 100 3 37 189 185 333 4.98e-13 68.6
Msa0220960 AT5G55730.1 31.169 154 100 3 37 189 185 333 4.98e-13 68.6

Find 94 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCCTGAGGGTGCTGGTGC+TGG 0.134067 2_1:+69497798 None:intergenic
AGGGTGCTGGTGCTGGGGCT+TGG 0.151988 2_1:+69497805 None:intergenic
AAGATTGCTGTGATGTTTAT+TGG 0.222736 2_1:+69497777 None:intergenic
GGCCTGAGGGTGCTGGTGCT+GGG 0.229466 2_1:+69497799 None:intergenic
GGGGAGGAATATTATTAATA+AGG 0.243127 2_1:+69497986 None:intergenic
TAATACCTGATGGAAGATTT+TGG 0.244650 2_1:+69497625 None:intergenic
CTTCTGGTAAAGAAGGTAAT+TGG 0.246525 2_1:-69497326 Msa0220960:CDS
GGGTGCTGGTGCTGGGGCTT+GGG 0.247233 2_1:+69497806 None:intergenic
CCAGAAGCTTGTGTTCTAAC+TGG 0.262827 2_1:+69497342 None:intergenic
TCATTTGATCTTCTTTGTCT+TGG 0.265276 2_1:-69496938 Msa0220960:three_prime_UTR
ATATTATTAATAAGGTAATT+TGG 0.268157 2_1:+69497994 None:intergenic
CATTTGATCTTCTTTGTCTT+GGG 0.284575 2_1:-69496937 Msa0220960:three_prime_UTR
TAACATTTGGTTGGAATGAT+AGG 0.285353 2_1:+69498131 None:intergenic
TCGGAAAGAGAGTAGTATTT+TGG 0.290230 2_1:+69497384 None:intergenic
CTGGTGCTGGGGCTTGGGCA+TGG 0.308800 2_1:+69497811 None:intergenic
GCAACCAACCAAAACAAATT+AGG 0.319290 2_1:-69498198 Msa0220960:five_prime_UTR
AAGGAATGAAGTTTAACATT+TGG 0.331747 2_1:+69498118 None:intergenic
GCTGGAAGGAATGTTGGTTT+TGG 0.343086 2_1:-69497039 Msa0220960:CDS
TCCTTTGGCTGTTTATCAAT+TGG 0.345240 2_1:-69497214 Msa0220960:CDS
TTGTATTCATTTGTCAACTA+AGG 0.352391 2_1:-69498070 Msa0220960:five_prime_UTR
AAGTCAAGCCTAATTTGTTT+TGG 0.353273 2_1:+69498190 None:intergenic
TTTGGATTTGTTGCTGGGCT+TGG 0.356493 2_1:-69497021 Msa0220960:CDS
TTCTGGTAAAGAAGGTAATT+GGG 0.362073 2_1:-69497325 Msa0220960:CDS
GGGGCTTGGGCATGGATTTG+TGG 0.365915 2_1:+69497819 None:intergenic
ATCACATCGAAGTTGCAATA+AGG 0.381378 2_1:+69498099 None:intergenic
AGATTGCTGTGATGTTTATT+GGG 0.382866 2_1:+69497778 None:intergenic
GTTTGAAACTGTTAAGAAAT+CGG 0.392584 2_1:+69497365 None:intergenic
GTGAGAAGAATGAGTGCAAC+AGG 0.394196 2_1:+69497861 None:intergenic
TTTGAGACAACAATTTCCTT+TGG 0.394518 2_1:-69497229 Msa0220960:CDS
TGTCTCAAAGCATTGTTGAT+AGG 0.394899 2_1:+69497243 None:intergenic
TTTGGAAGGCATTGTCAGTT+GGG 0.404503 2_1:+69497643 None:intergenic
GAGAATTTGTTGGCATGAGC+TGG 0.413850 2_1:+69498019 None:intergenic
AACCAAGTCAATGTTTCAAC+TGG 0.415400 2_1:-69497279 Msa0220960:CDS
AATGAAGTTTAACATTTGGT+TGG 0.422441 2_1:+69498122 None:intergenic
ATGATAGGAAATTACATGTT+GGG 0.430578 2_1:+69498146 None:intergenic
CTTCAGAAGTTGAGTTGAGT+TGG 0.431445 2_1:+69497682 None:intergenic
TAAGATTGGAAGATGAGGTT+TGG 0.432635 2_1:+69497929 None:intergenic
AACATTGACTTGGTTACCTT+GGG 0.438570 2_1:+69497287 None:intergenic
AAGTTGAGTTGAGTTGGGTT+TGG 0.439465 2_1:+69497688 None:intergenic
TTTATTGGGCCTGAGGGTGC+TGG 0.445785 2_1:+69497792 None:intergenic
GCCTGAGGGTGCTGGTGCTG+GGG 0.448279 2_1:+69497800 None:intergenic
TAGTGAAGGAGAATTAAGAT+TGG 0.450447 2_1:+69497915 None:intergenic
ATGGATTTGTGGAGTTAAGA+AGG 0.451732 2_1:+69497830 None:intergenic
TTCAGAAGTTGAGTTGAGTT+GGG 0.456224 2_1:+69497683 None:intergenic
TGGGCTTGGTTTGATTTGCA+TGG 0.457325 2_1:-69497007 Msa0220960:CDS
ACTCCGAGCAGCGACGTTGA+AGG 0.459155 2_1:-69497105 Msa0220960:CDS
CCAGTTAGAACACAAGCTTC+TGG 0.459667 2_1:-69497342 Msa0220960:CDS
CAGAAGCTTGTGTTCTAACT+GGG 0.462249 2_1:+69497343 None:intergenic
GCCCCAGCACCAGCACCCTC+AGG 0.463369 2_1:-69497801 Msa0220960:CDS
AAGAAGGATGATAGTGCAGC+TGG 0.464254 2_1:-69497057 Msa0220960:CDS
GGGCTTGGTTTGATTTGCAT+GGG 0.469150 2_1:-69497006 Msa0220960:CDS
AAACATTGACTTGGTTACCT+TGG 0.472673 2_1:+69497286 None:intergenic
AGTATGTGGAGAGGTGGAGA+GGG 0.475055 2_1:+69497954 None:intergenic
GCCTTCCAAAATCTTCCATC+AGG 0.478103 2_1:-69497630 Msa0220960:intron
TAATTTGGTTGAGAATTTGT+TGG 0.480655 2_1:+69498009 None:intergenic
TCATCCTTCTTACTAGAAGC+TGG 0.491718 2_1:+69497069 None:intergenic
ATCTCCAGCTTCTAGTAAGA+AGG 0.492739 2_1:-69497073 Msa0220960:CDS
TGTATTCATTTGTCAACTAA+GGG 0.493196 2_1:-69498069 Msa0220960:five_prime_UTR
AATGATAGGAAATTACATGT+TGG 0.497561 2_1:+69498145 None:intergenic
GAGTATGTGGAGAGGTGGAG+AGG 0.505022 2_1:+69497953 None:intergenic
CTCCAGTTGAAACATTGACT+TGG 0.506376 2_1:+69497277 None:intergenic
GAATGAGTTGAACTTTCTGT+TGG 0.508496 2_1:+69497421 None:intergenic
GGTTTGATTTGATATGAACC+TGG 0.511093 2_1:-69496916 Msa0220960:three_prime_UTR
AGAATTAAGATTGGAAGATG+AGG 0.512656 2_1:+69497924 None:intergenic
ACACAAGCTTCTGGTAAAGA+AGG 0.515910 2_1:-69497333 Msa0220960:CDS
CAACTCAACTCAACTTCTGA+AGG 0.527769 2_1:-69497681 Msa0220960:CDS
GGTCTTCACTTCACTTCCCA+AGG 0.534840 2_1:-69497303 Msa0220960:CDS
GATGAGGTTTGGTGAGTATG+TGG 0.538971 2_1:+69497940 None:intergenic
TAAGTCTCCATCTTCATCTC+CGG 0.540687 2_1:-69497157 Msa0220960:CDS
TTCTTACTAGAAGCTGGAGA+TGG 0.544262 2_1:+69497075 None:intergenic
TTTCATTGAGGAGACGAATG+AGG 0.550194 2_1:+69497730 None:intergenic
TCACCTTCAACGTCGCTGCT+CGG 0.550784 2_1:+69497102 None:intergenic
TGGATTTGTGGAGTTAAGAA+GGG 0.551170 2_1:+69497831 None:intergenic
TTAGGAGCCGGAGATGAAGA+TGG 0.552898 2_1:+69497150 None:intergenic
ATAAATACAAAGAATAAGCC+AGG 0.554395 2_1:+69496898 None:intergenic
GTGATGTTTATTGGGCCTGA+GGG 0.556035 2_1:+69497786 None:intergenic
TCCAATTGATAAACAGCCAA+AGG 0.570662 2_1:+69497213 None:intergenic
TTGGAAGGCATTGTCAGTTG+GGG 0.572321 2_1:+69497644 None:intergenic
TTGGTGAGTATGTGGAGAGG+TGG 0.574461 2_1:+69497948 None:intergenic
GGCTGTTTATCAATTGGACA+AGG 0.577680 2_1:-69497208 Msa0220960:CDS
ATTGAGGAGACGAATGAGGA+AGG 0.579677 2_1:+69497734 None:intergenic
TGTAGTGGTGAGTGCCATGT+TGG 0.588720 2_1:+69497887 None:intergenic
GAACTTTCTGTTGGTCTGAG+AGG 0.589738 2_1:+69497430 None:intergenic
GTATGTGGAGAGGTGGAGAG+GGG 0.592940 2_1:+69497955 None:intergenic
AGTGCAGCTGGAAGGAATGT+TGG 0.593131 2_1:-69497045 Msa0220960:CDS
TGTGATGTTTATTGGGCCTG+AGG 0.596385 2_1:+69497785 None:intergenic
AGTATAAATAAGTTTCACAT+TGG 0.607356 2_1:-69496800 Msa0220960:three_prime_UTR
CCATGTTGGTAAGTTAGTGA+AGG 0.621537 2_1:+69497901 None:intergenic
TCTGGTAAAGAAGGTAATTG+GGG 0.623440 2_1:-69497324 Msa0220960:CDS
CCTTCACTAACTTACCAACA+TGG 0.626947 2_1:-69497901 Msa0220960:exon
AGGATGATAGTGCAGCTGGA+AGG 0.628901 2_1:-69497053 Msa0220960:CDS
TGAGTTGTTGAGTTTCATTG+AGG 0.637415 2_1:+69497718 None:intergenic
GAGTGCAACAGGTGTTGTAG+TGG 0.647059 2_1:+69497872 None:intergenic
GGTTTGGTGAGTATGTGGAG+AGG 0.756503 2_1:+69497945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATTAATAAGGTAATT+TGG + chr2_1:69496990-69497009 None:intergenic 10.0%
!! ATATTATTAATAAGGTAATT+TGG + chr2_1:69496990-69497009 None:intergenic 10.0%
!! TTTATATATATAATACCTGA+TGG + chr2_1:69497369-69497388 None:intergenic 15.0%
!! TTTATATATATAATACCTGA+TGG + chr2_1:69497369-69497388 None:intergenic 15.0%
!! TACATGCAAGTTATAATTAT+AGG + chr2_1:69497481-69497500 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG - chr2_1:69497516-69497535 Msa0220960:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG - chr2_1:69498181-69498200 Msa0220960:five_prime_UTR 20.0%
!! TACATGCAAGTTATAATTAT+AGG + chr2_1:69497481-69497500 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG - chr2_1:69497516-69497535 Msa0220960:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG - chr2_1:69498181-69498200 Msa0220960:five_prime_UTR 20.0%
! ATGATAGGAAATTACATGTT+GGG + chr2_1:69496838-69496857 None:intergenic 25.0%
! AATGATAGGAAATTACATGT+TGG + chr2_1:69496839-69496858 None:intergenic 25.0%
! AATGAAGTTTAACATTTGGT+TGG + chr2_1:69496862-69496881 None:intergenic 25.0%
! AAGGAATGAAGTTTAACATT+TGG + chr2_1:69496866-69496885 None:intergenic 25.0%
! TTGTATTCATTTGTCAACTA+AGG - chr2_1:69496911-69496930 Msa0220960:three_prime_UTR 25.0%
! TGTATTCATTTGTCAACTAA+GGG - chr2_1:69496912-69496931 Msa0220960:three_prime_UTR 25.0%
! TAATTTGGTTGAGAATTTGT+TGG + chr2_1:69496975-69496994 None:intergenic 25.0%
!! GTTTGAAACTGTTAAGAAAT+CGG + chr2_1:69497619-69497638 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG + chr2_1:69498086-69498105 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG - chr2_1:69498136-69498155 Msa0220960:five_prime_UTR 25.0%
! ATGATAGGAAATTACATGTT+GGG + chr2_1:69496838-69496857 None:intergenic 25.0%
! AATGATAGGAAATTACATGT+TGG + chr2_1:69496839-69496858 None:intergenic 25.0%
! AATGAAGTTTAACATTTGGT+TGG + chr2_1:69496862-69496881 None:intergenic 25.0%
! AAGGAATGAAGTTTAACATT+TGG + chr2_1:69496866-69496885 None:intergenic 25.0%
! TTGTATTCATTTGTCAACTA+AGG - chr2_1:69496911-69496930 Msa0220960:three_prime_UTR 25.0%
! TGTATTCATTTGTCAACTAA+GGG - chr2_1:69496912-69496931 Msa0220960:three_prime_UTR 25.0%
! TAATTTGGTTGAGAATTTGT+TGG + chr2_1:69496975-69496994 None:intergenic 25.0%
!! GTTTGAAACTGTTAAGAAAT+CGG + chr2_1:69497619-69497638 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG + chr2_1:69498086-69498105 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG - chr2_1:69498136-69498155 Msa0220960:five_prime_UTR 25.0%
!! TTTTGGTTGGTTGCATTATA+TGG + chr2_1:69496777-69496796 None:intergenic 30.0%
!!! AAGTCAAGCCTAATTTGTTT+TGG + chr2_1:69496794-69496813 None:intergenic 30.0%
TAACATTTGGTTGGAATGAT+AGG + chr2_1:69496853-69496872 None:intergenic 30.0%
GGGGAGGAATATTATTAATA+AGG + chr2_1:69496998-69497017 None:intergenic 30.0%
AGAATTAAGATTGGAAGATG+AGG + chr2_1:69497060-69497079 None:intergenic 30.0%
TAGTGAAGGAGAATTAAGAT+TGG + chr2_1:69497069-69497088 None:intergenic 30.0%
AGATTGCTGTGATGTTTATT+GGG + chr2_1:69497206-69497225 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG + chr2_1:69497207-69497226 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG + chr2_1:69497359-69497378 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG + chr2_1:69497590-69497609 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG - chr2_1:69497656-69497675 Msa0220960:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG - chr2_1:69497752-69497771 Msa0220960:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG - chr2_1:69497798-69497817 Msa0220960:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG - chr2_1:69498043-69498062 Msa0220960:five_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG - chr2_1:69498044-69498063 Msa0220960:five_prime_UTR 30.0%
!! TTTTGGTTGGTTGCATTATA+TGG + chr2_1:69496777-69496796 None:intergenic 30.0%
!!! AAGTCAAGCCTAATTTGTTT+TGG + chr2_1:69496794-69496813 None:intergenic 30.0%
TAACATTTGGTTGGAATGAT+AGG + chr2_1:69496853-69496872 None:intergenic 30.0%
GGGGAGGAATATTATTAATA+AGG + chr2_1:69496998-69497017 None:intergenic 30.0%
AGAATTAAGATTGGAAGATG+AGG + chr2_1:69497060-69497079 None:intergenic 30.0%
TAGTGAAGGAGAATTAAGAT+TGG + chr2_1:69497069-69497088 None:intergenic 30.0%
AGATTGCTGTGATGTTTATT+GGG + chr2_1:69497206-69497225 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG + chr2_1:69497207-69497226 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG + chr2_1:69497359-69497378 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG + chr2_1:69497590-69497609 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG - chr2_1:69497656-69497675 Msa0220960:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG - chr2_1:69497752-69497771 Msa0220960:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG - chr2_1:69497798-69497817 Msa0220960:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG - chr2_1:69498043-69498062 Msa0220960:five_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG - chr2_1:69498044-69498063 Msa0220960:five_prime_UTR 30.0%
GCAACCAACCAAAACAAATT+AGG - chr2_1:69496783-69496802 Msa0220960:three_prime_UTR 35.0%
!!! CAAGCCTAATTTGTTTTGGT+TGG + chr2_1:69496790-69496809 None:intergenic 35.0%
ATCACATCGAAGTTGCAATA+AGG + chr2_1:69496885-69496904 None:intergenic 35.0%
TAAGATTGGAAGATGAGGTT+TGG + chr2_1:69497055-69497074 None:intergenic 35.0%
TGGATTTGTGGAGTTAAGAA+GGG + chr2_1:69497153-69497172 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG + chr2_1:69497154-69497173 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG - chr2_1:69497216-69497235 Msa0220960:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG + chr2_1:69497266-69497285 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG + chr2_1:69497301-69497320 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG + chr2_1:69497563-69497582 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG + chr2_1:69497600-69497619 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG - chr2_1:69497655-69497674 Msa0220960:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG - chr2_1:69497657-69497676 Msa0220960:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG + chr2_1:69497697-69497716 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG + chr2_1:69497698-69497717 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG - chr2_1:69497702-69497721 Msa0220960:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG + chr2_1:69497741-69497760 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG - chr2_1:69497767-69497786 Msa0220960:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG + chr2_1:69497771-69497790 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG + chr2_1:69497807-69497826 None:intergenic 35.0%
!!! TTGGTTTTGGATTTGTTGCT+GGG - chr2_1:69497955-69497974 Msa0220960:five_prime_UTR 35.0%
!! GGTTTGATTTGATATGAACC+TGG - chr2_1:69498065-69498084 Msa0220960:five_prime_UTR 35.0%
GCAACCAACCAAAACAAATT+AGG - chr2_1:69496783-69496802 Msa0220960:three_prime_UTR 35.0%
!!! CAAGCCTAATTTGTTTTGGT+TGG + chr2_1:69496790-69496809 None:intergenic 35.0%
ATCACATCGAAGTTGCAATA+AGG + chr2_1:69496885-69496904 None:intergenic 35.0%
TAAGATTGGAAGATGAGGTT+TGG + chr2_1:69497055-69497074 None:intergenic 35.0%
TGGATTTGTGGAGTTAAGAA+GGG + chr2_1:69497153-69497172 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG + chr2_1:69497154-69497173 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG - chr2_1:69497216-69497235 Msa0220960:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG + chr2_1:69497266-69497285 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG + chr2_1:69497301-69497320 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG + chr2_1:69497563-69497582 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG + chr2_1:69497600-69497619 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG - chr2_1:69497655-69497674 Msa0220960:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG - chr2_1:69497657-69497676 Msa0220960:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG + chr2_1:69497697-69497716 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG + chr2_1:69497698-69497717 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG - chr2_1:69497702-69497721 Msa0220960:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG + chr2_1:69497741-69497760 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG - chr2_1:69497767-69497786 Msa0220960:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG + chr2_1:69497771-69497790 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG + chr2_1:69497807-69497826 None:intergenic 35.0%
!!! TTGGTTTTGGATTTGTTGCT+GGG - chr2_1:69497955-69497974 Msa0220960:five_prime_UTR 35.0%
!! GGTTTGATTTGATATGAACC+TGG - chr2_1:69498065-69498084 Msa0220960:five_prime_UTR 35.0%
! CCATGTTGGTAAGTTAGTGA+AGG + chr2_1:69497083-69497102 None:intergenic 40.0%
CCTTCACTAACTTACCAACA+TGG - chr2_1:69497080-69497099 Msa0220960:CDS 40.0%
TTTCATTGAGGAGACGAATG+AGG + chr2_1:69497254-69497273 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG + chr2_1:69497296-69497315 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG + chr2_1:69497302-69497321 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG - chr2_1:69497300-69497319 Msa0220960:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG + chr2_1:69497341-69497360 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG + chr2_1:69497342-69497361 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG + chr2_1:69497355-69497374 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG + chr2_1:69497641-69497660 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG - chr2_1:69497648-69497667 Msa0220960:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG + chr2_1:69497707-69497726 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG - chr2_1:69497773-69497792 Msa0220960:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG - chr2_1:69497824-69497843 Msa0220960:CDS 40.0%
TTCTTACTAGAAGCTGGAGA+TGG + chr2_1:69497909-69497928 None:intergenic 40.0%
ATCTCCAGCTTCTAGTAAGA+AGG - chr2_1:69497908-69497927 Msa0220960:five_prime_UTR 40.0%
TCATCCTTCTTACTAGAAGC+TGG + chr2_1:69497915-69497934 None:intergenic 40.0%
!!! GTTGGTTTTGGATTTGTTGC+TGG - chr2_1:69497954-69497973 Msa0220960:five_prime_UTR 40.0%
! CCATGTTGGTAAGTTAGTGA+AGG + chr2_1:69497083-69497102 None:intergenic 40.0%
CCTTCACTAACTTACCAACA+TGG - chr2_1:69497080-69497099 Msa0220960:CDS 40.0%
TTTCATTGAGGAGACGAATG+AGG + chr2_1:69497254-69497273 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG + chr2_1:69497296-69497315 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG + chr2_1:69497302-69497321 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG - chr2_1:69497300-69497319 Msa0220960:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG + chr2_1:69497341-69497360 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG + chr2_1:69497342-69497361 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG + chr2_1:69497355-69497374 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG + chr2_1:69497641-69497660 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG - chr2_1:69497648-69497667 Msa0220960:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG + chr2_1:69497707-69497726 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG - chr2_1:69497773-69497792 Msa0220960:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG - chr2_1:69497824-69497843 Msa0220960:CDS 40.0%
TTCTTACTAGAAGCTGGAGA+TGG + chr2_1:69497909-69497928 None:intergenic 40.0%
ATCTCCAGCTTCTAGTAAGA+AGG - chr2_1:69497908-69497927 Msa0220960:five_prime_UTR 40.0%
TCATCCTTCTTACTAGAAGC+TGG + chr2_1:69497915-69497934 None:intergenic 40.0%
!!! GTTGGTTTTGGATTTGTTGC+TGG - chr2_1:69497954-69497973 Msa0220960:five_prime_UTR 40.0%
!! GAGAATTTGTTGGCATGAGC+TGG + chr2_1:69496965-69496984 None:intergenic 45.0%
!!! GTGGAGAGGGGTTTTATTTG+GGG + chr2_1:69497017-69497036 None:intergenic 45.0%
!!! GGTGGAGAGGGGTTTTATTT+GGG + chr2_1:69497018-69497037 None:intergenic 45.0%
!!! AGGTGGAGAGGGGTTTTATT+TGG + chr2_1:69497019-69497038 None:intergenic 45.0%
!! GATGAGGTTTGGTGAGTATG+TGG + chr2_1:69497044-69497063 None:intergenic 45.0%
GTGAGAAGAATGAGTGCAAC+AGG + chr2_1:69497123-69497142 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG + chr2_1:69497198-69497217 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG + chr2_1:69497199-69497218 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG + chr2_1:69497250-69497269 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG + chr2_1:69497340-69497359 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG - chr2_1:69497351-69497370 Msa0220960:CDS 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG + chr2_1:69497554-69497573 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG + chr2_1:69497642-69497661 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG - chr2_1:69497639-69497658 Msa0220960:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG + chr2_1:69497852-69497871 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG - chr2_1:69497924-69497943 Msa0220960:five_prime_UTR 45.0%
!!! GCTGGAAGGAATGTTGGTTT+TGG - chr2_1:69497942-69497961 Msa0220960:five_prime_UTR 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG - chr2_1:69497960-69497979 Msa0220960:five_prime_UTR 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG - chr2_1:69497974-69497993 Msa0220960:five_prime_UTR 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG - chr2_1:69497975-69497994 Msa0220960:five_prime_UTR 45.0%
!! GAGAATTTGTTGGCATGAGC+TGG + chr2_1:69496965-69496984 None:intergenic 45.0%
!!! GTGGAGAGGGGTTTTATTTG+GGG + chr2_1:69497017-69497036 None:intergenic 45.0%
!!! GGTGGAGAGGGGTTTTATTT+GGG + chr2_1:69497018-69497037 None:intergenic 45.0%
!!! AGGTGGAGAGGGGTTTTATT+TGG + chr2_1:69497019-69497038 None:intergenic 45.0%
!! GATGAGGTTTGGTGAGTATG+TGG + chr2_1:69497044-69497063 None:intergenic 45.0%
GTGAGAAGAATGAGTGCAAC+AGG + chr2_1:69497123-69497142 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG + chr2_1:69497198-69497217 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG + chr2_1:69497199-69497218 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG + chr2_1:69497250-69497269 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG + chr2_1:69497340-69497359 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG - chr2_1:69497351-69497370 Msa0220960:CDS 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG + chr2_1:69497554-69497573 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG + chr2_1:69497642-69497661 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG - chr2_1:69497639-69497658 Msa0220960:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG + chr2_1:69497852-69497871 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG - chr2_1:69497924-69497943 Msa0220960:five_prime_UTR 45.0%
!!! GCTGGAAGGAATGTTGGTTT+TGG - chr2_1:69497942-69497961 Msa0220960:five_prime_UTR 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG - chr2_1:69497960-69497979 Msa0220960:five_prime_UTR 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG - chr2_1:69497974-69497993 Msa0220960:five_prime_UTR 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG - chr2_1:69497975-69497994 Msa0220960:five_prime_UTR 45.0%
!!! ATAGTAATTTTTTTTATTAA+AGG + chr2_1:69498207-69498226 None:intergenic 5.0%
!!! ATAGTAATTTTTTTTATTAA+AGG + chr2_1:69498207-69498226 None:intergenic 5.0%
!!! GAGAGGGGTTTTATTTGGGG+AGG + chr2_1:69497014-69497033 None:intergenic 50.0%
AGTATGTGGAGAGGTGGAGA+GGG + chr2_1:69497030-69497049 None:intergenic 50.0%
TTGGTGAGTATGTGGAGAGG+TGG + chr2_1:69497036-69497055 None:intergenic 50.0%
!! GGTTTGGTGAGTATGTGGAG+AGG + chr2_1:69497039-69497058 None:intergenic 50.0%
TGTAGTGGTGAGTGCCATGT+TGG + chr2_1:69497097-69497116 None:intergenic 50.0%
! GAGTGCAACAGGTGTTGTAG+TGG + chr2_1:69497112-69497131 None:intergenic 50.0%
GGTCTTCACTTCACTTCCCA+AGG - chr2_1:69497678-69497697 Msa0220960:CDS 50.0%
TTAGGAGCCGGAGATGAAGA+TGG + chr2_1:69497834-69497853 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG + chr2_1:69497846-69497865 None:intergenic 50.0%
!!! TCGCTGCTCGGAGTTTTAGA+AGG + chr2_1:69497870-69497889 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG - chr2_1:69497928-69497947 Msa0220960:five_prime_UTR 50.0%
AGTGCAGCTGGAAGGAATGT+TGG - chr2_1:69497936-69497955 Msa0220960:five_prime_UTR 50.0%
!!! GAGAGGGGTTTTATTTGGGG+AGG + chr2_1:69497014-69497033 None:intergenic 50.0%
AGTATGTGGAGAGGTGGAGA+GGG + chr2_1:69497030-69497049 None:intergenic 50.0%
TTGGTGAGTATGTGGAGAGG+TGG + chr2_1:69497036-69497055 None:intergenic 50.0%
!! GGTTTGGTGAGTATGTGGAG+AGG + chr2_1:69497039-69497058 None:intergenic 50.0%
TGTAGTGGTGAGTGCCATGT+TGG + chr2_1:69497097-69497116 None:intergenic 50.0%
! GAGTGCAACAGGTGTTGTAG+TGG + chr2_1:69497112-69497131 None:intergenic 50.0%
GGTCTTCACTTCACTTCCCA+AGG - chr2_1:69497678-69497697 Msa0220960:CDS 50.0%
TTAGGAGCCGGAGATGAAGA+TGG + chr2_1:69497834-69497853 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG + chr2_1:69497846-69497865 None:intergenic 50.0%
!!! TCGCTGCTCGGAGTTTTAGA+AGG + chr2_1:69497870-69497889 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG - chr2_1:69497928-69497947 Msa0220960:five_prime_UTR 50.0%
AGTGCAGCTGGAAGGAATGT+TGG - chr2_1:69497936-69497955 Msa0220960:five_prime_UTR 50.0%
GTATGTGGAGAGGTGGAGAG+GGG + chr2_1:69497029-69497048 None:intergenic 55.0%
GAGTATGTGGAGAGGTGGAG+AGG + chr2_1:69497031-69497050 None:intergenic 55.0%
! TTTATTGGGCCTGAGGGTGC+TGG + chr2_1:69497192-69497211 None:intergenic 55.0%
TCACCTTCAACGTCGCTGCT+CGG + chr2_1:69497882-69497901 None:intergenic 55.0%
GTATGTGGAGAGGTGGAGAG+GGG + chr2_1:69497029-69497048 None:intergenic 55.0%
GAGTATGTGGAGAGGTGGAG+AGG + chr2_1:69497031-69497050 None:intergenic 55.0%
! TTTATTGGGCCTGAGGGTGC+TGG + chr2_1:69497192-69497211 None:intergenic 55.0%
TCACCTTCAACGTCGCTGCT+CGG + chr2_1:69497882-69497901 None:intergenic 55.0%
GGGGCTTGGGCATGGATTTG+TGG + chr2_1:69497165-69497184 None:intergenic 60.0%
ACTCCGAGCAGCGACGTTGA+AGG - chr2_1:69497876-69497895 Msa0220960:CDS 60.0%
GGGGCTTGGGCATGGATTTG+TGG + chr2_1:69497165-69497184 None:intergenic 60.0%
ACTCCGAGCAGCGACGTTGA+AGG - chr2_1:69497876-69497895 Msa0220960:CDS 60.0%
! CTGGTGCTGGGGCTTGGGCA+TGG + chr2_1:69497173-69497192 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG + chr2_1:69497178-69497197 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG + chr2_1:69497179-69497198 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG + chr2_1:69497184-69497203 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG + chr2_1:69497185-69497204 None:intergenic 70.0%
! CTGGTGCTGGGGCTTGGGCA+TGG + chr2_1:69497173-69497192 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG + chr2_1:69497178-69497197 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG + chr2_1:69497179-69497198 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG + chr2_1:69497184-69497203 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG + chr2_1:69497185-69497204 None:intergenic 70.0%
GCCCCAGCACCAGCACCCTC+AGG - chr2_1:69497180-69497199 Msa0220960:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG + chr2_1:69497186-69497205 None:intergenic 75.0%
GCCCCAGCACCAGCACCCTC+AGG - chr2_1:69497180-69497199 Msa0220960:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG + chr2_1:69497186-69497205 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69496769 69498234 69496769 ID=Msa0220960;Name=Msa0220960
chr2_1 mRNA 69496769 69498234 69496769 ID=Msa0220960-mRNA-1;Parent=Msa0220960;Name=Msa0220960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=330|1|1|1|1|1|2|224|248
chr2_1 exon 69496769 69497465 69496769 ID=Msa0220960-mRNA-1:exon:19693;Parent=Msa0220960-mRNA-1
chr2_1 exon 69497631 69498234 69497631 ID=Msa0220960-mRNA-1:exon:19692;Parent=Msa0220960-mRNA-1
chr2_1 five_prime_UTR 69497905 69498234 69497905 ID=Msa0220960-mRNA-1:five_prime_utr;Parent=Msa0220960-mRNA-1
chr2_1 CDS 69497631 69497904 69497631 ID=Msa0220960-mRNA-1:cds;Parent=Msa0220960-mRNA-1
chr2_1 CDS 69496993 69497465 69496993 ID=Msa0220960-mRNA-1:cds;Parent=Msa0220960-mRNA-1
chr2_1 three_prime_UTR 69496769 69496992 69496769 ID=Msa0220960-mRNA-1:three_prime_utr;Parent=Msa0220960-mRNA-1
Gene Sequence

>Msa0220960

ATGGCACTCACCACTACAACACCTGTTGCACTCATTCTTCTCACTCTCATTCCCTTCTTAACTCCACAAATCCATGCCCAAGCCCCAGCACCAGCACCCTCAGGCCCAATAAACATCACAGCAATCTTTGAAAAAGCTGGTCAATACACCTTCCTCATTCGTCTCCTCAATGAAACTCAACAACTCACTCAAATCCAAACCCAACTCAACTCAACTTCTGAAGGTTTCACAATCTTTGCCCCAACTGACAATGCCTTCCAAAATCTTCCATCAGGAGCAATCAACGACCTCTCAGACCAACAGAAAGTTCAACTCATTCTCTACCATGTTACACCAAAATACTACTCTCTTTCCGATTTCTTAACAGTTTCAAACCCAGTTAGAACACAAGCTTCTGGTAAAGAAGGTAATTGGGGTCTTCACTTCACTTCCCAAGGTAACCAAGTCAATGTTTCAACTGGAGTTGTTACTGTACCTATCAACAATGCTTTGAGACAACAATTTCCTTTGGCTGTTTATCAATTGGACAAGGTTTTGTTACCTTCTGAACTTTTTGGTGCTAAGTCTCCATCTTCATCTCCGGCTCCTAAGTCGTCGAAAACTCCTTCTAAAACTCCGAGCAGCGACGTTGAAGGTGATGCTCCATCTCCAGCTTCTAGTAAGAAGGATGATAGTGCAGCTGGAAGGAATGTTGGTTTTGGATTTGTTGCTGGGCTTGGTTTGATTTGCATGGGAGTTCTTTTTTGA
Protein sequence

>Msa0220960

MALTTTTPVALILLTLIPFLTPQIHAQAPAPAPSGPINITAIFEKAGQYTFLIRLLNETQQLTQIQTQLNSTSEGFTIFAPTDNAFQNLPSGAINDLSDQQKVQLILYHVTPKYYSLSDFLTVSNPVRTQASGKEGNWGLHFTSQGNQVNVSTGVVTVPINNALRQQFPLAVYQLDKVLLPSELFGAKSPSSSPAPKSSKTPSKTPSSDVEGDAPSPASSKKDDSAAGRNVGFGFVAGLGLICMGVLF*