Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0320440 | KEH37197.1 | 80.000 | 65 | 13 | 0 | 1 | 65 | 1 | 65 | 3.48e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0198440 | Msa0320440 | 0.804579 | 2.018959e-49 | -8.615850e-47 |
| Msa0200370 | Msa0320440 | 0.979323 | 1.021822e-147 | -8.615850e-47 |
| Msa0200870 | Msa0320440 | 0.809140 | 2.192614e-50 | -8.615850e-47 |
| Msa0243470 | Msa0320440 | 0.824796 | 6.647926e-54 | -8.615850e-47 |
| Msa0278670 | Msa0320440 | 0.807094 | 5.977721e-50 | -8.615850e-47 |
| Msa0281270 | Msa0320440 | 0.978690 | 2.352181e-146 | -8.615850e-47 |
| Msa0281940 | Msa0320440 | 0.834570 | 2.776548e-56 | -8.615850e-47 |
| Msa0282170 | Msa0320440 | 0.827656 | 1.387141e-54 | -8.615850e-47 |
| Msa0309550 | Msa0320440 | 0.841353 | 5.001820e-58 | -8.615850e-47 |
| Msa0318070 | Msa0320440 | 0.807428 | 5.079884e-50 | -8.615850e-47 |
| Msa0320440 | Msa0337740 | -0.801304 | 9.592103e-49 | -8.615850e-47 |
| Msa0320440 | Msa0363460 | 0.807222 | 5.615500e-50 | -8.615850e-47 |
| Msa0320440 | Msa0411000 | 0.806518 | 7.911577e-50 | -8.615850e-47 |
| Msa0320440 | Msa0418430 | 0.809932 | 1.482127e-50 | -8.615850e-47 |
| Msa0320440 | Msa0419620 | -0.845944 | 2.960637e-59 | -8.615850e-47 |
| Msa0320440 | Msa0455230 | 0.807791 | 4.253753e-50 | -8.615850e-47 |
| Msa0320440 | Msa0511720 | -0.827038 | 1.950147e-54 | -8.615850e-47 |
| Msa0320440 | Msa0530950 | 0.812107 | 5.005802e-51 | -8.615850e-47 |
| Msa0320440 | Msa0576600 | 0.802873 | 4.562543e-49 | -8.615850e-47 |
| Msa0320440 | Msa0628100 | 0.813830 | 2.098747e-51 | -8.615850e-47 |
| Msa0320440 | Msa0656270 | 0.800368 | 1.489550e-48 | -8.615850e-47 |
| Msa0320440 | Msa0722930 | 0.822773 | 1.979299e-53 | -8.615850e-47 |
| Msa0320440 | Msa0723140 | 0.814124 | 1.807492e-51 | -8.615850e-47 |
| Msa0320440 | Msa0724050 | 0.800931 | 1.143289e-48 | -8.615850e-47 |
| Msa0320440 | Msa0801360 | 0.801831 | 7.479609e-49 | -8.615850e-47 |
| Msa0320440 | Msa0807050 | 0.822792 | 1.959917e-53 | -8.615850e-47 |
| Msa0320440 | Msa0832990 | 0.823432 | 1.389287e-53 | -8.615850e-47 |
| Msa0320440 | Msa1132950 | 0.832078 | 1.160463e-55 | -8.615850e-47 |
| Msa0320440 | Msa1282920 | 0.803322 | 3.684485e-49 | -8.615850e-47 |
| Msa0320440 | Msa1292730 | 0.808461 | 3.062587e-50 | -8.615850e-47 |
| Msa0320440 | Msa1305750 | 0.800004 | 1.765879e-48 | -8.615850e-47 |
| Msa0320440 | Msa1421870 | 0.801118 | 1.046954e-48 | -8.615850e-47 |
| Msa0320440 | Msa1432790 | 0.988352 | 1.109971e-173 | -8.615850e-47 |
| Msa0320440 | Msa1432800 | 0.955128 | 6.175160e-113 | -8.615850e-47 |
| Msa0320440 | Msa1450020 | 0.824023 | 1.010230e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0320440 | MtrunA17_Chr2g0295501 | 89.286 | 56 | 6 | 0 | 1 | 56 | 1 | 56 | 2.45e-31 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATGCAAGATCGTGCTTC+TGG | 0.277502 | 2_4:+20933364 | Msa0320440:CDS |
| CTAAGGAAGATTGGCTTAGA+AGG | 0.290742 | 2_4:+20933298 | Msa0320440:CDS |
| TGCATGCTGACCCTTCTTGT+TGG | 0.323679 | 2_4:-20933349 | None:intergenic |
| GCATGCAAGATCGTGCTTCT+GGG | 0.397696 | 2_4:+20933365 | Msa0320440:CDS |
| TCATCAGACCTAAGGAAGAT+TGG | 0.412607 | 2_4:+20933289 | Msa0320440:CDS |
| CTTCTAAGCCAATCTTCCTT+AGG | 0.445559 | 2_4:-20933297 | None:intergenic |
| GCTTCTGGGGTTAGCAAGGA+TGG | 0.500998 | 2_4:+20933379 | Msa0320440:CDS |
| TAAGGAAGATTGGCTTAGAA+GGG | 0.540665 | 2_4:+20933299 | Msa0320440:CDS |
| CACCTCCTTCATCAGACCTA+AGG | 0.563117 | 2_4:+20933281 | Msa0320440:CDS |
| CATGCAAGATCGTGCTTCTG+GGG | 0.577242 | 2_4:+20933366 | Msa0320440:CDS |
| TCGTGCTTCTGGGGTTAGCA+AGG | 0.589442 | 2_4:+20933375 | Msa0320440:CDS |
| ATCTTCCTTAGGTCTGATGA+AGG | 0.601958 | 2_4:-20933286 | None:intergenic |
| TTCCTTAGGTCTGATGAAGG+AGG | 0.672818 | 2_4:-20933283 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAGTGCTTAAAATTTAAA+GGG | - | chr2_4:20933472-20933491 | None:intergenic | 20.0% |
| !! | AAGTAGTGCTTAAAATTTAA+AGG | - | chr2_4:20933473-20933492 | None:intergenic | 20.0% |
| !! | GCTTTAATAGACAAGTTTTA+TGG | + | chr2_4:20933325-20933344 | Msa0320440:CDS | 25.0% |
| !! | ACTTTGCCTTAAATTTTAAG+TGG | + | chr2_4:20933489-20933508 | Msa0320440:intron | 25.0% |
| !!! | CTTTGCCTTAAATTTTAAGT+GGG | + | chr2_4:20933490-20933509 | Msa0320440:intron | 25.0% |
| TGAGACCCACTTAAAATTTA+AGG | - | chr2_4:20933498-20933517 | None:intergenic | 30.0% | |
| TATGTAGAAAAAAAGCTCGA+AGG | + | chr2_4:20933540-20933559 | Msa0320440:intron | 30.0% | |
| !! | TAAGGAAGATTGGCTTAGAA+GGG | + | chr2_4:20933299-20933318 | Msa0320440:CDS | 35.0% |
| !! | AGTTTTATGGTCCAACAAGA+AGG | + | chr2_4:20933338-20933357 | Msa0320440:CDS | 35.0% |
| ! | GTTTTATGGTCCAACAAGAA+GGG | + | chr2_4:20933339-20933358 | Msa0320440:CDS | 35.0% |
| ATCGAATGTAAGTTACCCAA+AGG | + | chr2_4:20933423-20933442 | Msa0320440:intron | 35.0% | |
| TCGAATGTAAGTTACCCAAA+GGG | + | chr2_4:20933424-20933443 | Msa0320440:intron | 35.0% | |
| ATCTTCCTTAGGTCTGATGA+AGG | - | chr2_4:20933289-20933308 | None:intergenic | 40.0% | |
| TCATCAGACCTAAGGAAGAT+TGG | + | chr2_4:20933289-20933308 | Msa0320440:CDS | 40.0% | |
| CTTCTAAGCCAATCTTCCTT+AGG | - | chr2_4:20933300-20933319 | None:intergenic | 40.0% | |
| !! | CTAAGGAAGATTGGCTTAGA+AGG | + | chr2_4:20933298-20933317 | Msa0320440:CDS | 40.0% |
| CGAATGTAAGTTACCCAAAG+GGG | + | chr2_4:20933425-20933444 | Msa0320440:intron | 40.0% | |
| TGGGTCTCAGTTTCATGTTA+TGG | + | chr2_4:20933509-20933528 | Msa0320440:intron | 40.0% | |
| TTCCTTAGGTCTGATGAAGG+AGG | - | chr2_4:20933286-20933305 | None:intergenic | 45.0% | |
| !! | AACGATTTTGGGTCCCCTTT+GGG | - | chr2_4:20933441-20933460 | None:intergenic | 45.0% |
| AAAGGGGACCCAAAATCGTT+GGG | + | chr2_4:20933441-20933460 | Msa0320440:intron | 45.0% | |
| CACCTCCTTCATCAGACCTA+AGG | + | chr2_4:20933281-20933300 | Msa0320440:CDS | 50.0% | |
| TGCATGCTGACCCTTCTTGT+TGG | - | chr2_4:20933352-20933371 | None:intergenic | 50.0% | |
| AGCATGCAAGATCGTGCTTC+TGG | + | chr2_4:20933364-20933383 | Msa0320440:CDS | 50.0% | |
| GCATGCAAGATCGTGCTTCT+GGG | + | chr2_4:20933365-20933384 | Msa0320440:CDS | 50.0% | |
| CATGCAAGATCGTGCTTCTG+GGG | + | chr2_4:20933366-20933385 | Msa0320440:CDS | 50.0% | |
| !! | CAACGATTTTGGGTCCCCTT+TGG | - | chr2_4:20933442-20933461 | None:intergenic | 50.0% |
| CAAAGGGGACCCAAAATCGT+TGG | + | chr2_4:20933440-20933459 | Msa0320440:intron | 50.0% | |
| AAGGGGACCCAAAATCGTTG+GGG | + | chr2_4:20933442-20933461 | Msa0320440:intron | 50.0% | |
| ! | GGGTCTACCCCAACGATTTT+GGG | - | chr2_4:20933452-20933471 | None:intergenic | 50.0% |
| ! | AGGGTCTACCCCAACGATTT+TGG | - | chr2_4:20933453-20933472 | None:intergenic | 50.0% |
| !! | TCGTGCTTCTGGGGTTAGCA+AGG | + | chr2_4:20933375-20933394 | Msa0320440:CDS | 55.0% |
| !! | GCTTCTGGGGTTAGCAAGGA+TGG | + | chr2_4:20933379-20933398 | Msa0320440:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 20933277 | 20933597 | 20933277 | ID=Msa0320440;Name=Msa0320440 |
| chr2_4 | mRNA | 20933277 | 20933597 | 20933277 | ID=Msa0320440-mRNA-1;Parent=Msa0320440;Name=Msa0320440-mRNA-1;_AED=0.10;_eAED=0.32;_QI=0|0|0|1|0|0.5|2|0|67 |
| chr2_4 | exon | 20933277 | 20933429 | 20933277 | ID=Msa0320440-mRNA-1:exon:7681;Parent=Msa0320440-mRNA-1 |
| chr2_4 | exon | 20933547 | 20933597 | 20933547 | ID=Msa0320440-mRNA-1:exon:7682;Parent=Msa0320440-mRNA-1 |
| chr2_4 | CDS | 20933277 | 20933429 | 20933277 | ID=Msa0320440-mRNA-1:cds;Parent=Msa0320440-mRNA-1 |
| chr2_4 | CDS | 20933547 | 20933597 | 20933547 | ID=Msa0320440-mRNA-1:cds;Parent=Msa0320440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
