AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1433900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433900 MtrunA17_Chr2g0325021 97.590 249 5 1 1 248 1 249 2.96e-176 484
Msa1433900 MtrunA17_Chr7g0263981 50.289 173 84 2 16 186 17 189 1.29e-51 168
Msa1433900 MtrunA17_Chr4g0029331 49.444 180 86 2 14 188 33 212 5.18e-48 159
Msa1433900 MtrunA17_Chr4g0029501 36.889 225 131 3 31 244 66 290 8.46e-41 141
Msa1433900 MtrunA17_Chr2g0326431 48.649 148 76 0 38 185 41 188 3.79e-40 139
Msa1433900 MtrunA17_Chr4g0029441 40.000 190 108 2 31 214 66 255 1.49e-38 135
Msa1433900 MtrunA17_Chr4g0026611 43.229 192 99 5 27 211 63 251 2.21e-38 135
Msa1433900 MtrunA17_Chr8g0379621 37.402 254 136 10 14 247 66 316 1.26e-35 129
Msa1433900 MtrunA17_Chr8g0379631 37.008 254 137 10 14 247 66 316 2.83e-35 127
Msa1433900 MtrunA17_Chr2g0326421 35.294 221 138 4 29 248 60 276 1.32e-33 122
Msa1433900 MtrunA17_Chr2g0284411 40.449 178 102 3 38 213 68 243 5.19e-33 121
Msa1433900 MtrunA17_Chr2g0284431 40.449 178 102 3 38 213 89 264 5.70e-33 121
Msa1433900 MtrunA17_Chr2g0284451 36.818 220 118 6 20 219 50 268 3.75e-32 119
Msa1433900 MtrunA17_Chr8g0379611 35.000 220 138 4 31 246 70 288 1.67e-31 118
Msa1433900 MtrunA17_Chr4g0029431 38.650 163 99 1 27 188 56 218 2.58e-30 114
Msa1433900 MtrunA17_Chr2g0326411 40.789 152 89 1 38 188 69 220 2.97e-30 114
Msa1433900 MtrunA17_Chr7g0246041 37.198 207 115 6 12 218 161 352 6.97e-29 112
Msa1433900 MtrunA17_Chr5g0448001 35.057 174 107 3 37 209 47 215 1.01e-28 109
Msa1433900 MtrunA17_Chr5g0448211 30.612 245 137 8 26 247 185 419 8.65e-19 84.7
Msa1433900 MtrunA17_Chr2g0296571 28.755 233 151 7 8 234 164 387 3.69e-14 71.2
Msa1433900 MtrunA17_Chr3g0102421 28.105 153 96 4 38 185 193 336 5.47e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433900 AT2G20520.1 52.869 244 101 5 12 248 9 245 1.05e-73 225
Msa1433900 AT1G03870.1 56.279 215 86 5 37 248 38 247 7.50e-72 220
Msa1433900 AT5G44130.1 56.889 225 92 3 6 226 3 226 7.32e-70 215
Msa1433900 AT5G03170.1 56.593 182 76 3 25 204 23 203 2.15e-61 193
Msa1433900 AT5G60490.1 47.682 151 78 1 36 185 37 187 1.51e-41 142
Msa1433900 AT2G04780.1 34.896 192 116 5 37 223 45 232 4.41e-24 97.4
Msa1433900 AT2G04780.2 34.896 192 116 5 37 223 45 232 4.41e-24 97.4
Msa1433900 AT3G60900.1 33.000 200 123 4 18 217 171 359 1.26e-23 99.0
Msa1433900 AT3G46550.1 29.487 234 152 6 20 248 190 415 2.21e-18 84.0
Msa1433900 AT2G45470.1 32.278 158 102 2 30 186 180 333 2.68e-18 83.6
Msa1433900 AT4G12730.1 37.349 166 92 7 25 187 178 334 2.55e-17 80.9
Msa1433900 AT5G55730.2 31.169 154 100 3 37 189 185 333 4.98e-13 68.6
Msa1433900 AT5G55730.1 31.169 154 100 3 37 189 185 333 4.98e-13 68.6

Find 94 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCCTGAGGGTGCTGGTGC+TGG 0.134067 tig0027435:-36105 None:intergenic
AGGGTGCTGGTGCTGGGGCT+TGG 0.151988 tig0027435:-36098 None:intergenic
AAGATTGCTGTGATGTTTAT+TGG 0.222736 tig0027435:-36126 None:intergenic
GGCCTGAGGGTGCTGGTGCT+GGG 0.229466 tig0027435:-36104 None:intergenic
GGGGAGGAATATTATTAATA+AGG 0.243127 tig0027435:-35917 None:intergenic
TAATACCTGATGGAAGATTT+TGG 0.244650 tig0027435:-36278 None:intergenic
CTTCTGGTAAAGAAGGTAAT+TGG 0.246525 tig0027435:+36577 Msa1433900:CDS
GGGTGCTGGTGCTGGGGCTT+GGG 0.247233 tig0027435:-36097 None:intergenic
CCAGAAGCTTGTGTTCTAAC+TGG 0.262827 tig0027435:-36561 None:intergenic
TCATTTGATCTTCTTTGTCT+TGG 0.265276 tig0027435:+36965 Msa1433900:three_prime_UTR
ATATTATTAATAAGGTAATT+TGG 0.268157 tig0027435:-35909 None:intergenic
CATTTGATCTTCTTTGTCTT+GGG 0.284575 tig0027435:+36966 Msa1433900:three_prime_UTR
TAACATTTGGTTGGAATGAT+AGG 0.285353 tig0027435:-35772 None:intergenic
TCGGAAAGAGAGTAGTATTT+TGG 0.290230 tig0027435:-36519 None:intergenic
CTGGTGCTGGGGCTTGGGCA+TGG 0.308800 tig0027435:-36092 None:intergenic
GCAACCAACCAAAACAAATT+AGG 0.319290 tig0027435:+35705 Msa1433900:five_prime_UTR
AAGGAATGAAGTTTAACATT+TGG 0.331747 tig0027435:-35785 None:intergenic
GCTGGAAGGAATGTTGGTTT+TGG 0.343086 tig0027435:+36864 Msa1433900:CDS
TCCTTTGGCTGTTTATCAAT+TGG 0.345240 tig0027435:+36689 Msa1433900:CDS
TTGTATTCATTTGTCAACTA+AGG 0.352391 tig0027435:+35833 Msa1433900:five_prime_UTR
AAGTCAAGCCTAATTTGTTT+TGG 0.353273 tig0027435:-35713 None:intergenic
TTTGGATTTGTTGCTGGGCT+TGG 0.356493 tig0027435:+36882 Msa1433900:CDS
TTCTGGTAAAGAAGGTAATT+GGG 0.362073 tig0027435:+36578 Msa1433900:CDS
GGGGCTTGGGCATGGATTTG+TGG 0.365915 tig0027435:-36084 None:intergenic
ATCACATCGAAGTTGCAATA+AGG 0.381378 tig0027435:-35804 None:intergenic
AGATTGCTGTGATGTTTATT+GGG 0.382866 tig0027435:-36125 None:intergenic
GTTTGAAACTGTTAAGAAAT+CGG 0.392584 tig0027435:-36538 None:intergenic
GTGAGAAGAATGAGTGCAAC+AGG 0.394196 tig0027435:-36042 None:intergenic
TTTGAGACAACAATTTCCTT+TGG 0.394518 tig0027435:+36674 Msa1433900:CDS
TGTCTCAAAGCATTGTTGAT+AGG 0.394899 tig0027435:-36660 None:intergenic
TTTGGAAGGCATTGTCAGTT+GGG 0.404503 tig0027435:-36260 None:intergenic
GAGAATTTGTTGGCATGAGC+TGG 0.413850 tig0027435:-35884 None:intergenic
AACCAAGTCAATGTTTCAAC+TGG 0.415400 tig0027435:+36624 Msa1433900:CDS
AATGAAGTTTAACATTTGGT+TGG 0.422441 tig0027435:-35781 None:intergenic
ATGATAGGAAATTACATGTT+GGG 0.430578 tig0027435:-35757 None:intergenic
CTTCAGAAGTTGAGTTGAGT+TGG 0.431445 tig0027435:-36221 None:intergenic
TAAGATTGGAAGATGAGGTT+TGG 0.432635 tig0027435:-35974 None:intergenic
AACATTGACTTGGTTACCTT+GGG 0.438570 tig0027435:-36616 None:intergenic
AAGTTGAGTTGAGTTGGGTT+TGG 0.439465 tig0027435:-36215 None:intergenic
TTTATTGGGCCTGAGGGTGC+TGG 0.445785 tig0027435:-36111 None:intergenic
GCCTGAGGGTGCTGGTGCTG+GGG 0.448279 tig0027435:-36103 None:intergenic
TAGTGAAGGAGAATTAAGAT+TGG 0.450447 tig0027435:-35988 None:intergenic
ATGGATTTGTGGAGTTAAGA+AGG 0.451732 tig0027435:-36073 None:intergenic
TTCAGAAGTTGAGTTGAGTT+GGG 0.456224 tig0027435:-36220 None:intergenic
TGGGCTTGGTTTGATTTGCA+TGG 0.457325 tig0027435:+36896 Msa1433900:CDS
ACTCCGAGCAGCGACGTTGA+AGG 0.459155 tig0027435:+36798 Msa1433900:CDS
CCAGTTAGAACACAAGCTTC+TGG 0.459667 tig0027435:+36561 Msa1433900:CDS
CAGAAGCTTGTGTTCTAACT+GGG 0.462249 tig0027435:-36560 None:intergenic
GCCCCAGCACCAGCACCCTC+AGG 0.463369 tig0027435:+36102 Msa1433900:CDS
AAGAAGGATGATAGTGCAGC+TGG 0.464254 tig0027435:+36846 Msa1433900:CDS
GGGCTTGGTTTGATTTGCAT+GGG 0.469150 tig0027435:+36897 Msa1433900:CDS
AAACATTGACTTGGTTACCT+TGG 0.472673 tig0027435:-36617 None:intergenic
AGTATGTGGAGAGGTGGAGA+GGG 0.475055 tig0027435:-35949 None:intergenic
GCCTTCCAAAATCTTCCATC+AGG 0.478103 tig0027435:+36273 Msa1433900:CDS
TAATTTGGTTGAGAATTTGT+TGG 0.480655 tig0027435:-35894 None:intergenic
TCATCCTTCTTACTAGAAGC+TGG 0.491718 tig0027435:-36834 None:intergenic
ATCTCCAGCTTCTAGTAAGA+AGG 0.492739 tig0027435:+36830 Msa1433900:CDS
TGTATTCATTTGTCAACTAA+GGG 0.493196 tig0027435:+35834 Msa1433900:five_prime_UTR
AATGATAGGAAATTACATGT+TGG 0.497561 tig0027435:-35758 None:intergenic
GAGTATGTGGAGAGGTGGAG+AGG 0.505022 tig0027435:-35950 None:intergenic
CTCCAGTTGAAACATTGACT+TGG 0.506376 tig0027435:-36626 None:intergenic
GAATGAGTTGAACTTTCTGT+TGG 0.508496 tig0027435:-36482 None:intergenic
GGTTTGATTTGATATGAACC+TGG 0.511093 tig0027435:+36987 Msa1433900:three_prime_UTR
AGAATTAAGATTGGAAGATG+AGG 0.512656 tig0027435:-35979 None:intergenic
ACACAAGCTTCTGGTAAAGA+AGG 0.515910 tig0027435:+36570 Msa1433900:CDS
CAACTCAACTCAACTTCTGA+AGG 0.527769 tig0027435:+36222 Msa1433900:CDS
GGTCTTCACTTCACTTCCCA+AGG 0.534840 tig0027435:+36600 Msa1433900:CDS
GATGAGGTTTGGTGAGTATG+TGG 0.538971 tig0027435:-35963 None:intergenic
TAAGTCTCCATCTTCATCTC+CGG 0.540687 tig0027435:+36746 Msa1433900:CDS
TTCTTACTAGAAGCTGGAGA+TGG 0.544262 tig0027435:-36828 None:intergenic
TTTCATTGAGGAGACGAATG+AGG 0.550194 tig0027435:-36173 None:intergenic
TCACCTTCAACGTCGCTGCT+CGG 0.550784 tig0027435:-36801 None:intergenic
TGGATTTGTGGAGTTAAGAA+GGG 0.551170 tig0027435:-36072 None:intergenic
TTAGGAGCCGGAGATGAAGA+TGG 0.552898 tig0027435:-36753 None:intergenic
ATAAATACAAAGAATAAGCC+AGG 0.554395 tig0027435:-37005 None:intergenic
GTGATGTTTATTGGGCCTGA+GGG 0.556035 tig0027435:-36117 None:intergenic
TCCAATTGATAAACAGCCAA+AGG 0.570662 tig0027435:-36690 None:intergenic
TTGGAAGGCATTGTCAGTTG+GGG 0.572321 tig0027435:-36259 None:intergenic
TTGGTGAGTATGTGGAGAGG+TGG 0.574461 tig0027435:-35955 None:intergenic
GGCTGTTTATCAATTGGACA+AGG 0.577680 tig0027435:+36695 Msa1433900:CDS
ATTGAGGAGACGAATGAGGA+AGG 0.579677 tig0027435:-36169 None:intergenic
TGTAGTGGTGAGTGCCATGT+TGG 0.588720 tig0027435:-36016 None:intergenic
GAACTTTCTGTTGGTCTGAG+AGG 0.589738 tig0027435:-36473 None:intergenic
GTATGTGGAGAGGTGGAGAG+GGG 0.592940 tig0027435:-35948 None:intergenic
AGTGCAGCTGGAAGGAATGT+TGG 0.593131 tig0027435:+36858 Msa1433900:CDS
TGTGATGTTTATTGGGCCTG+AGG 0.596385 tig0027435:-36118 None:intergenic
AGTATAAATAAGTTTCACAT+TGG 0.607356 tig0027435:+37103 Msa1433900:three_prime_UTR
CCATGTTGGTAAGTTAGTGA+AGG 0.621537 tig0027435:-36002 None:intergenic
TCTGGTAAAGAAGGTAATTG+GGG 0.623440 tig0027435:+36579 Msa1433900:CDS
CCTTCACTAACTTACCAACA+TGG 0.626947 tig0027435:+36002 Msa1433900:exon
AGGATGATAGTGCAGCTGGA+AGG 0.628901 tig0027435:+36850 Msa1433900:CDS
TGAGTTGTTGAGTTTCATTG+AGG 0.637415 tig0027435:-36185 None:intergenic
GAGTGCAACAGGTGTTGTAG+TGG 0.647059 tig0027435:-36031 None:intergenic
GGTTTGGTGAGTATGTGGAG+AGG 0.756503 tig0027435:-35958 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATTAATAAGGTAATT+TGG - tig0027435:35912-35931 None:intergenic 10.0%
!! ATATTATTAATAAGGTAATT+TGG - tig0027435:35912-35931 None:intergenic 10.0%
!! TTTATATATATAATACCTGA+TGG - tig0027435:36291-36310 None:intergenic 15.0%
!! TTTATATATATAATACCTGA+TGG - tig0027435:36291-36310 None:intergenic 15.0%
!! TACATGCAAGTTATAATTAT+AGG - tig0027435:36403-36422 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG + tig0027435:36438-36457 Msa1433900:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG + tig0027435:37103-37122 Msa1433900:three_prime_UTR 20.0%
!! TACATGCAAGTTATAATTAT+AGG - tig0027435:36403-36422 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG + tig0027435:36438-36457 Msa1433900:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG + tig0027435:37103-37122 Msa1433900:three_prime_UTR 20.0%
! ATGATAGGAAATTACATGTT+GGG - tig0027435:35760-35779 None:intergenic 25.0%
! AATGATAGGAAATTACATGT+TGG - tig0027435:35761-35780 None:intergenic 25.0%
! AATGAAGTTTAACATTTGGT+TGG - tig0027435:35784-35803 None:intergenic 25.0%
! AAGGAATGAAGTTTAACATT+TGG - tig0027435:35788-35807 None:intergenic 25.0%
! TTGTATTCATTTGTCAACTA+AGG + tig0027435:35833-35852 Msa1433900:five_prime_UTR 25.0%
! TGTATTCATTTGTCAACTAA+GGG + tig0027435:35834-35853 Msa1433900:five_prime_UTR 25.0%
! TAATTTGGTTGAGAATTTGT+TGG - tig0027435:35897-35916 None:intergenic 25.0%
!! GTTTGAAACTGTTAAGAAAT+CGG - tig0027435:36541-36560 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG - tig0027435:37008-37027 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG + tig0027435:37058-37077 Msa1433900:three_prime_UTR 25.0%
! ATGATAGGAAATTACATGTT+GGG - tig0027435:35760-35779 None:intergenic 25.0%
! AATGATAGGAAATTACATGT+TGG - tig0027435:35761-35780 None:intergenic 25.0%
! AATGAAGTTTAACATTTGGT+TGG - tig0027435:35784-35803 None:intergenic 25.0%
! AAGGAATGAAGTTTAACATT+TGG - tig0027435:35788-35807 None:intergenic 25.0%
! TTGTATTCATTTGTCAACTA+AGG + tig0027435:35833-35852 Msa1433900:five_prime_UTR 25.0%
! TGTATTCATTTGTCAACTAA+GGG + tig0027435:35834-35853 Msa1433900:five_prime_UTR 25.0%
! TAATTTGGTTGAGAATTTGT+TGG - tig0027435:35897-35916 None:intergenic 25.0%
!! GTTTGAAACTGTTAAGAAAT+CGG - tig0027435:36541-36560 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG - tig0027435:37008-37027 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG + tig0027435:37058-37077 Msa1433900:three_prime_UTR 25.0%
!! TTTTGGTTGGTTGCATTATA+TGG - tig0027435:35699-35718 None:intergenic 30.0%
!!! AAGTCAAGCCTAATTTGTTT+TGG - tig0027435:35716-35735 None:intergenic 30.0%
TAACATTTGGTTGGAATGAT+AGG - tig0027435:35775-35794 None:intergenic 30.0%
GGGGAGGAATATTATTAATA+AGG - tig0027435:35920-35939 None:intergenic 30.0%
AGAATTAAGATTGGAAGATG+AGG - tig0027435:35982-36001 None:intergenic 30.0%
TAGTGAAGGAGAATTAAGAT+TGG - tig0027435:35991-36010 None:intergenic 30.0%
AGATTGCTGTGATGTTTATT+GGG - tig0027435:36128-36147 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG - tig0027435:36129-36148 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG - tig0027435:36281-36300 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG - tig0027435:36512-36531 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG + tig0027435:36578-36597 Msa1433900:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG + tig0027435:36674-36693 Msa1433900:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG + tig0027435:36720-36739 Msa1433900:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG + tig0027435:36965-36984 Msa1433900:three_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG + tig0027435:36966-36985 Msa1433900:three_prime_UTR 30.0%
!! TTTTGGTTGGTTGCATTATA+TGG - tig0027435:35699-35718 None:intergenic 30.0%
!!! AAGTCAAGCCTAATTTGTTT+TGG - tig0027435:35716-35735 None:intergenic 30.0%
TAACATTTGGTTGGAATGAT+AGG - tig0027435:35775-35794 None:intergenic 30.0%
GGGGAGGAATATTATTAATA+AGG - tig0027435:35920-35939 None:intergenic 30.0%
AGAATTAAGATTGGAAGATG+AGG - tig0027435:35982-36001 None:intergenic 30.0%
TAGTGAAGGAGAATTAAGAT+TGG - tig0027435:35991-36010 None:intergenic 30.0%
AGATTGCTGTGATGTTTATT+GGG - tig0027435:36128-36147 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG - tig0027435:36129-36148 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG - tig0027435:36281-36300 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG - tig0027435:36512-36531 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG + tig0027435:36578-36597 Msa1433900:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG + tig0027435:36674-36693 Msa1433900:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG + tig0027435:36720-36739 Msa1433900:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG + tig0027435:36965-36984 Msa1433900:three_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG + tig0027435:36966-36985 Msa1433900:three_prime_UTR 30.0%
GCAACCAACCAAAACAAATT+AGG + tig0027435:35705-35724 Msa1433900:five_prime_UTR 35.0%
!!! CAAGCCTAATTTGTTTTGGT+TGG - tig0027435:35712-35731 None:intergenic 35.0%
ATCACATCGAAGTTGCAATA+AGG - tig0027435:35807-35826 None:intergenic 35.0%
TAAGATTGGAAGATGAGGTT+TGG - tig0027435:35977-35996 None:intergenic 35.0%
TGGATTTGTGGAGTTAAGAA+GGG - tig0027435:36075-36094 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG - tig0027435:36076-36095 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG + tig0027435:36138-36157 Msa1433900:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG - tig0027435:36188-36207 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG - tig0027435:36223-36242 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG - tig0027435:36485-36504 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG - tig0027435:36522-36541 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG + tig0027435:36577-36596 Msa1433900:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG + tig0027435:36579-36598 Msa1433900:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG - tig0027435:36619-36638 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG - tig0027435:36620-36639 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG + tig0027435:36624-36643 Msa1433900:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG - tig0027435:36663-36682 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG + tig0027435:36689-36708 Msa1433900:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG - tig0027435:36693-36712 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG - tig0027435:36729-36748 None:intergenic 35.0%
!!! TTGGTTTTGGATTTGTTGCT+GGG + tig0027435:36877-36896 Msa1433900:CDS 35.0%
!! GGTTTGATTTGATATGAACC+TGG + tig0027435:36987-37006 Msa1433900:three_prime_UTR 35.0%
GCAACCAACCAAAACAAATT+AGG + tig0027435:35705-35724 Msa1433900:five_prime_UTR 35.0%
!!! CAAGCCTAATTTGTTTTGGT+TGG - tig0027435:35712-35731 None:intergenic 35.0%
ATCACATCGAAGTTGCAATA+AGG - tig0027435:35807-35826 None:intergenic 35.0%
TAAGATTGGAAGATGAGGTT+TGG - tig0027435:35977-35996 None:intergenic 35.0%
TGGATTTGTGGAGTTAAGAA+GGG - tig0027435:36075-36094 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG - tig0027435:36076-36095 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG + tig0027435:36138-36157 Msa1433900:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG - tig0027435:36188-36207 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG - tig0027435:36223-36242 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG - tig0027435:36485-36504 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG - tig0027435:36522-36541 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG + tig0027435:36577-36596 Msa1433900:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG + tig0027435:36579-36598 Msa1433900:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG - tig0027435:36619-36638 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG - tig0027435:36620-36639 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG + tig0027435:36624-36643 Msa1433900:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG - tig0027435:36663-36682 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG + tig0027435:36689-36708 Msa1433900:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG - tig0027435:36693-36712 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG - tig0027435:36729-36748 None:intergenic 35.0%
!!! TTGGTTTTGGATTTGTTGCT+GGG + tig0027435:36877-36896 Msa1433900:CDS 35.0%
!! GGTTTGATTTGATATGAACC+TGG + tig0027435:36987-37006 Msa1433900:three_prime_UTR 35.0%
! CCATGTTGGTAAGTTAGTGA+AGG - tig0027435:36005-36024 None:intergenic 40.0%
CCTTCACTAACTTACCAACA+TGG + tig0027435:36002-36021 Msa1433900:exon 40.0%
TTTCATTGAGGAGACGAATG+AGG - tig0027435:36176-36195 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG - tig0027435:36218-36237 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG - tig0027435:36224-36243 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG + tig0027435:36222-36241 Msa1433900:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG - tig0027435:36263-36282 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG - tig0027435:36264-36283 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG - tig0027435:36277-36296 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG - tig0027435:36563-36582 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG + tig0027435:36570-36589 Msa1433900:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG - tig0027435:36629-36648 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG + tig0027435:36695-36714 Msa1433900:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG + tig0027435:36746-36765 Msa1433900:CDS 40.0%
TTCTTACTAGAAGCTGGAGA+TGG - tig0027435:36831-36850 None:intergenic 40.0%
ATCTCCAGCTTCTAGTAAGA+AGG + tig0027435:36830-36849 Msa1433900:CDS 40.0%
TCATCCTTCTTACTAGAAGC+TGG - tig0027435:36837-36856 None:intergenic 40.0%
!!! GTTGGTTTTGGATTTGTTGC+TGG + tig0027435:36876-36895 Msa1433900:CDS 40.0%
! CCATGTTGGTAAGTTAGTGA+AGG - tig0027435:36005-36024 None:intergenic 40.0%
CCTTCACTAACTTACCAACA+TGG + tig0027435:36002-36021 Msa1433900:exon 40.0%
TTTCATTGAGGAGACGAATG+AGG - tig0027435:36176-36195 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG - tig0027435:36218-36237 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG - tig0027435:36224-36243 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG + tig0027435:36222-36241 Msa1433900:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG - tig0027435:36263-36282 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG - tig0027435:36264-36283 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG - tig0027435:36277-36296 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG - tig0027435:36563-36582 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG + tig0027435:36570-36589 Msa1433900:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG - tig0027435:36629-36648 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG + tig0027435:36695-36714 Msa1433900:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG + tig0027435:36746-36765 Msa1433900:CDS 40.0%
TTCTTACTAGAAGCTGGAGA+TGG - tig0027435:36831-36850 None:intergenic 40.0%
ATCTCCAGCTTCTAGTAAGA+AGG + tig0027435:36830-36849 Msa1433900:CDS 40.0%
TCATCCTTCTTACTAGAAGC+TGG - tig0027435:36837-36856 None:intergenic 40.0%
!!! GTTGGTTTTGGATTTGTTGC+TGG + tig0027435:36876-36895 Msa1433900:CDS 40.0%
!! GAGAATTTGTTGGCATGAGC+TGG - tig0027435:35887-35906 None:intergenic 45.0%
!!! GTGGAGAGGGGTTTTATTTG+GGG - tig0027435:35939-35958 None:intergenic 45.0%
!!! GGTGGAGAGGGGTTTTATTT+GGG - tig0027435:35940-35959 None:intergenic 45.0%
!!! AGGTGGAGAGGGGTTTTATT+TGG - tig0027435:35941-35960 None:intergenic 45.0%
!! GATGAGGTTTGGTGAGTATG+TGG - tig0027435:35966-35985 None:intergenic 45.0%
GTGAGAAGAATGAGTGCAAC+AGG - tig0027435:36045-36064 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG - tig0027435:36120-36139 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG - tig0027435:36121-36140 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG - tig0027435:36172-36191 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG - tig0027435:36262-36281 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG + tig0027435:36273-36292 Msa1433900:CDS 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG - tig0027435:36476-36495 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG - tig0027435:36564-36583 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG + tig0027435:36561-36580 Msa1433900:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG - tig0027435:36774-36793 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG + tig0027435:36846-36865 Msa1433900:CDS 45.0%
!!! GCTGGAAGGAATGTTGGTTT+TGG + tig0027435:36864-36883 Msa1433900:CDS 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG + tig0027435:36882-36901 Msa1433900:CDS 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG + tig0027435:36896-36915 Msa1433900:CDS 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG + tig0027435:36897-36916 Msa1433900:CDS 45.0%
!! GAGAATTTGTTGGCATGAGC+TGG - tig0027435:35887-35906 None:intergenic 45.0%
!!! GTGGAGAGGGGTTTTATTTG+GGG - tig0027435:35939-35958 None:intergenic 45.0%
!!! GGTGGAGAGGGGTTTTATTT+GGG - tig0027435:35940-35959 None:intergenic 45.0%
!!! AGGTGGAGAGGGGTTTTATT+TGG - tig0027435:35941-35960 None:intergenic 45.0%
!! GATGAGGTTTGGTGAGTATG+TGG - tig0027435:35966-35985 None:intergenic 45.0%
GTGAGAAGAATGAGTGCAAC+AGG - tig0027435:36045-36064 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG - tig0027435:36120-36139 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG - tig0027435:36121-36140 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG - tig0027435:36172-36191 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG - tig0027435:36262-36281 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG + tig0027435:36273-36292 Msa1433900:CDS 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG - tig0027435:36476-36495 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG - tig0027435:36564-36583 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG + tig0027435:36561-36580 Msa1433900:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG - tig0027435:36774-36793 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG + tig0027435:36846-36865 Msa1433900:CDS 45.0%
!!! GCTGGAAGGAATGTTGGTTT+TGG + tig0027435:36864-36883 Msa1433900:CDS 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG + tig0027435:36882-36901 Msa1433900:CDS 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG + tig0027435:36896-36915 Msa1433900:CDS 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG + tig0027435:36897-36916 Msa1433900:CDS 45.0%
!!! ATAGTAATTTTTTTTATTAA+AGG - tig0027435:37129-37148 None:intergenic 5.0%
!!! ATAGTAATTTTTTTTATTAA+AGG - tig0027435:37129-37148 None:intergenic 5.0%
!!! GAGAGGGGTTTTATTTGGGG+AGG - tig0027435:35936-35955 None:intergenic 50.0%
AGTATGTGGAGAGGTGGAGA+GGG - tig0027435:35952-35971 None:intergenic 50.0%
TTGGTGAGTATGTGGAGAGG+TGG - tig0027435:35958-35977 None:intergenic 50.0%
!! GGTTTGGTGAGTATGTGGAG+AGG - tig0027435:35961-35980 None:intergenic 50.0%
TGTAGTGGTGAGTGCCATGT+TGG - tig0027435:36019-36038 None:intergenic 50.0%
! GAGTGCAACAGGTGTTGTAG+TGG - tig0027435:36034-36053 None:intergenic 50.0%
GGTCTTCACTTCACTTCCCA+AGG + tig0027435:36600-36619 Msa1433900:CDS 50.0%
TTAGGAGCCGGAGATGAAGA+TGG - tig0027435:36756-36775 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG - tig0027435:36768-36787 None:intergenic 50.0%
!!! TCGCTGCTCGGAGTTTTAGA+AGG - tig0027435:36792-36811 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG + tig0027435:36850-36869 Msa1433900:CDS 50.0%
AGTGCAGCTGGAAGGAATGT+TGG + tig0027435:36858-36877 Msa1433900:CDS 50.0%
!!! GAGAGGGGTTTTATTTGGGG+AGG - tig0027435:35936-35955 None:intergenic 50.0%
AGTATGTGGAGAGGTGGAGA+GGG - tig0027435:35952-35971 None:intergenic 50.0%
TTGGTGAGTATGTGGAGAGG+TGG - tig0027435:35958-35977 None:intergenic 50.0%
!! GGTTTGGTGAGTATGTGGAG+AGG - tig0027435:35961-35980 None:intergenic 50.0%
TGTAGTGGTGAGTGCCATGT+TGG - tig0027435:36019-36038 None:intergenic 50.0%
! GAGTGCAACAGGTGTTGTAG+TGG - tig0027435:36034-36053 None:intergenic 50.0%
GGTCTTCACTTCACTTCCCA+AGG + tig0027435:36600-36619 Msa1433900:CDS 50.0%
TTAGGAGCCGGAGATGAAGA+TGG - tig0027435:36756-36775 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG - tig0027435:36768-36787 None:intergenic 50.0%
!!! TCGCTGCTCGGAGTTTTAGA+AGG - tig0027435:36792-36811 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG + tig0027435:36850-36869 Msa1433900:CDS 50.0%
AGTGCAGCTGGAAGGAATGT+TGG + tig0027435:36858-36877 Msa1433900:CDS 50.0%
GTATGTGGAGAGGTGGAGAG+GGG - tig0027435:35951-35970 None:intergenic 55.0%
GAGTATGTGGAGAGGTGGAG+AGG - tig0027435:35953-35972 None:intergenic 55.0%
! TTTATTGGGCCTGAGGGTGC+TGG - tig0027435:36114-36133 None:intergenic 55.0%
TCACCTTCAACGTCGCTGCT+CGG - tig0027435:36804-36823 None:intergenic 55.0%
GTATGTGGAGAGGTGGAGAG+GGG - tig0027435:35951-35970 None:intergenic 55.0%
GAGTATGTGGAGAGGTGGAG+AGG - tig0027435:35953-35972 None:intergenic 55.0%
! TTTATTGGGCCTGAGGGTGC+TGG - tig0027435:36114-36133 None:intergenic 55.0%
TCACCTTCAACGTCGCTGCT+CGG - tig0027435:36804-36823 None:intergenic 55.0%
GGGGCTTGGGCATGGATTTG+TGG - tig0027435:36087-36106 None:intergenic 60.0%
ACTCCGAGCAGCGACGTTGA+AGG + tig0027435:36798-36817 Msa1433900:CDS 60.0%
GGGGCTTGGGCATGGATTTG+TGG - tig0027435:36087-36106 None:intergenic 60.0%
ACTCCGAGCAGCGACGTTGA+AGG + tig0027435:36798-36817 Msa1433900:CDS 60.0%
! CTGGTGCTGGGGCTTGGGCA+TGG - tig0027435:36095-36114 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG - tig0027435:36100-36119 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG - tig0027435:36101-36120 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG - tig0027435:36106-36125 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG - tig0027435:36107-36126 None:intergenic 70.0%
! CTGGTGCTGGGGCTTGGGCA+TGG - tig0027435:36095-36114 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG - tig0027435:36100-36119 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG - tig0027435:36101-36120 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG - tig0027435:36106-36125 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG - tig0027435:36107-36126 None:intergenic 70.0%
GCCCCAGCACCAGCACCCTC+AGG + tig0027435:36102-36121 Msa1433900:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG - tig0027435:36108-36127 None:intergenic 75.0%
GCCCCAGCACCAGCACCCTC+AGG + tig0027435:36102-36121 Msa1433900:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG - tig0027435:36108-36127 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0027435 gene 35691 37156 35691 ID=Msa1433900;Name=Msa1433900
tig0027435 mRNA 35691 37156 35691 ID=Msa1433900-mRNA-1;Parent=Msa1433900;Name=Msa1433900-mRNA-1;_AED=0.00;_eAED=0.00;_QI=330|1|1|1|1|1|2|224|248
tig0027435 exon 35691 36294 35691 ID=Msa1433900-mRNA-1:exon:8885;Parent=Msa1433900-mRNA-1
tig0027435 exon 36460 37156 36460 ID=Msa1433900-mRNA-1:exon:8886;Parent=Msa1433900-mRNA-1
tig0027435 five_prime_UTR 35691 36020 35691 ID=Msa1433900-mRNA-1:five_prime_utr;Parent=Msa1433900-mRNA-1
tig0027435 CDS 36021 36294 36021 ID=Msa1433900-mRNA-1:cds;Parent=Msa1433900-mRNA-1
tig0027435 CDS 36460 36932 36460 ID=Msa1433900-mRNA-1:cds;Parent=Msa1433900-mRNA-1
tig0027435 three_prime_UTR 36933 37156 36933 ID=Msa1433900-mRNA-1:three_prime_utr;Parent=Msa1433900-mRNA-1
Gene Sequence

>Msa1433900

ATGGCACTCACCACTACAACACCTGTTGCACTCATTCTTCTCACTCTCATTCCCTTCTTAACTCCACAAATCCATGCCCAAGCCCCAGCACCAGCACCCTCAGGCCCAATAAACATCACAGCAATCTTTGAAAAAGCTGGTCAATACACCTTCCTCATTCGTCTCCTCAATGAAACTCAACAACTCACTCAAATCCAAACCCAACTCAACTCAACTTCTGAAGGTTTCACAATCTTTGCCCCAACTGACAATGCCTTCCAAAATCTTCCATCAGGAGCAATCAACGACCTCTCAGACCAACAGAAAGTTCAACTCATTCTCTACCATGTTACACCAAAATACTACTCTCTTTCCGATTTCTTAACAGTTTCAAACCCAGTTAGAACACAAGCTTCTGGTAAAGAAGGTAATTGGGGTCTTCACTTCACTTCCCAAGGTAACCAAGTCAATGTTTCAACTGGAGTTGTTACTGTACCTATCAACAATGCTTTGAGACAACAATTTCCTTTGGCTGTTTATCAATTGGACAAGGTTTTGTTACCTTCTGAACTTTTTGGTGCTAAGTCTCCATCTTCATCTCCGGCTCCTAAGTCGTCGAAAACTCCTTCTAAAACTCCGAGCAGCGACGTTGAAGGTGATGCTCCATCTCCAGCTTCTAGTAAGAAGGATGATAGTGCAGCTGGAAGGAATGTTGGTTTTGGATTTGTTGCTGGGCTTGGTTTGATTTGCATGGGAGTTCTTTTTTGA
Protein sequence

>Msa1433900

MALTTTTPVALILLTLIPFLTPQIHAQAPAPAPSGPINITAIFEKAGQYTFLIRLLNETQQLTQIQTQLNSTSEGFTIFAPTDNAFQNLPSGAINDLSDQQKVQLILYHVTPKYYSLSDFLTVSNPVRTQASGKEGNWGLHFTSQGNQVNVSTGVVTVPINNALRQQFPLAVYQLDKVLLPSELFGAKSPSSSPAPKSSKTPSKTPSSDVEGDAPSPASSKKDDSAAGRNVGFGFVAGLGLICMGVLF*