AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300310 MtrunA17_Chr2g0325021 95.833 240 9 1 1 239 1 240 4.23e-165 456
Msa0300310 MtrunA17_Chr7g0263981 50.289 173 84 2 16 186 17 189 1.49e-51 168
Msa0300310 MtrunA17_Chr4g0029331 49.444 180 86 2 14 188 33 212 4.05e-48 159
Msa0300310 MtrunA17_Chr2g0326431 48.649 148 76 0 38 185 41 188 3.24e-40 139
Msa0300310 MtrunA17_Chr4g0029501 37.557 221 127 3 31 240 66 286 3.57e-40 139
Msa0300310 MtrunA17_Chr4g0029441 40.000 190 108 2 31 214 66 255 1.03e-38 135
Msa0300310 MtrunA17_Chr4g0026611 43.784 185 97 4 31 211 70 251 1.31e-38 135
Msa0300310 MtrunA17_Chr8g0379621 42.458 179 93 6 14 184 66 242 3.11e-35 127
Msa0300310 MtrunA17_Chr8g0379631 41.899 179 94 6 14 184 66 242 1.70e-34 125
Msa0300310 MtrunA17_Chr2g0284411 40.449 178 102 3 38 213 68 243 6.32e-33 120
Msa0300310 MtrunA17_Chr2g0284431 40.449 178 102 3 38 213 89 264 7.23e-33 120
Msa0300310 MtrunA17_Chr2g0284451 35.685 241 132 7 20 240 50 287 1.79e-32 119
Msa0300310 MtrunA17_Chr2g0326421 36.000 200 127 1 29 227 60 259 3.75e-32 118
Msa0300310 MtrunA17_Chr8g0379611 43.421 152 85 1 31 181 70 221 1.36e-31 118
Msa0300310 MtrunA17_Chr2g0326411 40.789 152 89 1 38 188 69 220 2.29e-30 114
Msa0300310 MtrunA17_Chr4g0029431 38.650 163 99 1 27 188 56 218 2.62e-30 114
Msa0300310 MtrunA17_Chr7g0246041 37.198 207 115 6 12 218 161 352 7.53e-29 112
Msa0300310 MtrunA17_Chr5g0448001 35.057 174 107 3 37 209 47 215 1.30e-28 108
Msa0300310 MtrunA17_Chr5g0448211 31.064 235 129 8 26 237 185 409 1.31e-18 84.0
Msa0300310 MtrunA17_Chr2g0296571 29.630 189 123 6 8 190 164 348 2.81e-14 71.6
Msa0300310 MtrunA17_Chr3g0102421 28.105 153 96 4 38 185 193 336 5.54e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300310 AT2G20520.1 56.109 221 90 4 12 225 9 229 1.62e-71 219
Msa0300310 AT5G44130.1 56.889 225 92 3 6 226 3 226 3.68e-70 215
Msa0300310 AT1G03870.1 57.005 207 81 5 37 240 38 239 6.52e-69 212
Msa0300310 AT5G03170.1 56.593 182 76 3 25 204 23 203 1.38e-61 194
Msa0300310 AT5G60490.1 47.682 151 78 1 36 185 37 187 1.11e-41 142
Msa0300310 AT2G04780.1 34.783 207 119 6 37 238 45 240 4.11e-24 97.1
Msa0300310 AT2G04780.2 34.783 207 119 6 37 238 45 240 4.11e-24 97.1
Msa0300310 AT3G60900.1 33.000 200 123 4 18 217 171 359 9.07e-24 99.0
Msa0300310 AT2G45470.1 32.278 158 102 2 30 186 180 333 2.17e-18 84.0
Msa0300310 AT3G46550.1 31.579 171 109 5 20 185 190 357 3.01e-18 83.2
Msa0300310 AT4G12730.1 37.576 165 91 7 26 187 179 334 1.92e-17 80.9
Msa0300310 AT5G55730.2 31.169 154 100 3 37 189 185 333 5.16e-13 68.2
Msa0300310 AT5G55730.1 31.169 154 100 3 37 189 185 333 5.16e-13 68.2

Find 96 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCCTGAGGGTGCTGGTGC+TGG 0.134067 2_3:-55656931 None:intergenic
AGGGTGCTGGTGCTGGGGCT+TGG 0.151988 2_3:-55656924 None:intergenic
AAGATTGCTGTGATGTTTAT+TGG 0.222736 2_3:-55656952 None:intergenic
GGCCTGAGGGTGCTGGTGCT+GGG 0.229466 2_3:-55656930 None:intergenic
GGGGAGGAATATTATTAATA+AGG 0.243127 2_3:-55656743 None:intergenic
TAATACCTGATGGAAGATTT+TGG 0.244650 2_3:-55657104 None:intergenic
CTTCTGGTAAAGAAGGTAAT+TGG 0.246525 2_3:+55657403 Msa0300310:CDS
GGGTGCTGGTGCTGGGGCTT+GGG 0.247233 2_3:-55656923 None:intergenic
CCAGAAGCTTGTGTTCTAAC+TGG 0.262827 2_3:-55657387 None:intergenic
TGTTGGTTTGGATTTGTTGC+TGG 0.265011 2_3:+55657701 Msa0300310:CDS
TCATTTGATCTTCTTTGTCT+TGG 0.265276 2_3:+55657791 Msa0300310:three_prime_UTR
ATATTATTAATAAGGTAATT+TGG 0.268157 2_3:-55656735 None:intergenic
CATTTGATCTTCTTTGTCTT+GGG 0.284575 2_3:+55657792 Msa0300310:three_prime_UTR
TAACATTTGGTTGGAATGAT+AGG 0.285353 2_3:-55656598 None:intergenic
TCGGAAAGAGAGTAGTATTT+TGG 0.290230 2_3:-55657345 None:intergenic
CTGGTGCTGGGGCTTGGGCA+TGG 0.308800 2_3:-55656918 None:intergenic
GCAACCAACCAAAACAAATT+AGG 0.319290 2_3:+55656531 Msa0300310:five_prime_UTR
AAGGAATGAAGTTTAACATT+TGG 0.331747 2_3:-55656611 None:intergenic
TCCTTTGGCTGTTTATCAAT+TGG 0.345240 2_3:+55657515 Msa0300310:CDS
TTGTATTCATTTGTCAACTA+AGG 0.352391 2_3:+55656659 Msa0300310:five_prime_UTR
AAGTCAAGCCTAATTTGTTT+TGG 0.353273 2_3:-55656539 None:intergenic
TTCTGGTAAAGAAGGTAATT+GGG 0.362073 2_3:+55657404 Msa0300310:CDS
GGGGCTTGGGCATGGATTTG+TGG 0.365915 2_3:-55656910 None:intergenic
TTTGGATTTGTTGCTGGGCT+TGG 0.372505 2_3:+55657707 Msa0300310:CDS
ATCACATCGAAGTTGCAATA+AGG 0.381378 2_3:-55656630 None:intergenic
AGATTGCTGTGATGTTTATT+GGG 0.382866 2_3:-55656951 None:intergenic
GTTTGAAACTGTTAAGAAAT+CGG 0.392584 2_3:-55657364 None:intergenic
GTGAGAAGAATGAGTGCAAC+AGG 0.394196 2_3:-55656868 None:intergenic
TTTGAGACAACAATTTCCTT+TGG 0.394518 2_3:+55657500 Msa0300310:CDS
TGTCTCAAAGCATTGTTGAT+AGG 0.394899 2_3:-55657486 None:intergenic
TTTGGAAGGCATTGTCAGTT+GGG 0.404503 2_3:-55657086 None:intergenic
GTTGGTTTGGATTTGTTGCT+GGG 0.413627 2_3:+55657702 Msa0300310:CDS
GAGAATTTGTTGGCATGAGC+TGG 0.413850 2_3:-55656710 None:intergenic
AACCAAGTCAATGTTTCAAC+TGG 0.415400 2_3:+55657450 Msa0300310:CDS
AATGAAGTTTAACATTTGGT+TGG 0.422441 2_3:-55656607 None:intergenic
ATGATAGGAAATTACATGTT+GGG 0.430578 2_3:-55656583 None:intergenic
CTTCAGAAGTTGAGTTGAGT+TGG 0.431445 2_3:-55657047 None:intergenic
TAAGATTGGAAGATGAGGTT+TGG 0.432635 2_3:-55656800 None:intergenic
AACATTGACTTGGTTACCTT+GGG 0.438570 2_3:-55657442 None:intergenic
AAGTTGAGTTGAGTTGGGTT+TGG 0.439465 2_3:-55657041 None:intergenic
TTTATTGGGCCTGAGGGTGC+TGG 0.445785 2_3:-55656937 None:intergenic
GCCTGAGGGTGCTGGTGCTG+GGG 0.448279 2_3:-55656929 None:intergenic
TAGTGAAGGAGAATTAAGAT+TGG 0.450447 2_3:-55656814 None:intergenic
ATGGATTTGTGGAGTTAAGA+AGG 0.451732 2_3:-55656899 None:intergenic
TTCAGAAGTTGAGTTGAGTT+GGG 0.456224 2_3:-55657046 None:intergenic
TGGGCTTGGTTTGATTTGCA+TGG 0.457325 2_3:+55657721 Msa0300310:exon
ACTCCGAGCAGCGACGTTGA+AGG 0.459155 2_3:+55657624 Msa0300310:CDS
CCAGTTAGAACACAAGCTTC+TGG 0.459667 2_3:+55657387 Msa0300310:CDS
CAGAAGCTTGTGTTCTAACT+GGG 0.462249 2_3:-55657386 None:intergenic
AGCTGGAAGGAATGTTGGTT+TGG 0.462893 2_3:+55657689 Msa0300310:CDS
GCCCCAGCACCAGCACCCTC+AGG 0.463369 2_3:+55656928 Msa0300310:CDS
AAGAAGGATGATAGTGCAGC+TGG 0.464254 2_3:+55657672 Msa0300310:CDS
GGGCTTGGTTTGATTTGCAT+GGG 0.469150 2_3:+55657722 Msa0300310:exon
AAACATTGACTTGGTTACCT+TGG 0.472673 2_3:-55657443 None:intergenic
AGTATGTGGAGAGGTGGAGA+GGG 0.475055 2_3:-55656775 None:intergenic
GCCTTCCAAAATCTTCCATC+AGG 0.478103 2_3:+55657099 Msa0300310:CDS
TAATTTGGTTGAGAATTTGT+TGG 0.480655 2_3:-55656720 None:intergenic
TCATCCTTCTTACTAGAAGC+TGG 0.491718 2_3:-55657660 None:intergenic
ATCTCCAGCTTCTAGTAAGA+AGG 0.492739 2_3:+55657656 Msa0300310:CDS
TGTATTCATTTGTCAACTAA+GGG 0.493196 2_3:+55656660 Msa0300310:five_prime_UTR
AATGATAGGAAATTACATGT+TGG 0.497561 2_3:-55656584 None:intergenic
GAGTATGTGGAGAGGTGGAG+AGG 0.505022 2_3:-55656776 None:intergenic
CTCCAGTTGAAACATTGACT+TGG 0.506376 2_3:-55657452 None:intergenic
GAATGAGTTGAACTTTCTGT+TGG 0.508496 2_3:-55657308 None:intergenic
GGTTTGATTTGATATGAACC+TGG 0.511093 2_3:+55657813 Msa0300310:three_prime_UTR
AGAATTAAGATTGGAAGATG+AGG 0.512656 2_3:-55656805 None:intergenic
ACACAAGCTTCTGGTAAAGA+AGG 0.515910 2_3:+55657396 Msa0300310:CDS
CAACTCAACTCAACTTCTGA+AGG 0.527769 2_3:+55657048 Msa0300310:CDS
GGTCTTCACTTCACTTCCCA+AGG 0.534840 2_3:+55657426 Msa0300310:CDS
GATGAGGTTTGGTGAGTATG+TGG 0.538971 2_3:-55656789 None:intergenic
TAAGTCTCCATCTTCATCTC+CGG 0.540687 2_3:+55657572 Msa0300310:CDS
TTCTTACTAGAAGCTGGAGA+TGG 0.544262 2_3:-55657654 None:intergenic
TTTCATTGAGGAGACGAATG+AGG 0.550194 2_3:-55656999 None:intergenic
TCACCTTCAACGTCGCTGCT+CGG 0.550784 2_3:-55657627 None:intergenic
TGGATTTGTGGAGTTAAGAA+GGG 0.551170 2_3:-55656898 None:intergenic
TTAGGAGCCGGAGATGAAGA+TGG 0.552898 2_3:-55657579 None:intergenic
ATAAATACAAAGAATAAGCC+AGG 0.554395 2_3:-55657831 None:intergenic
GTGATGTTTATTGGGCCTGA+GGG 0.556035 2_3:-55656943 None:intergenic
TCCAATTGATAAACAGCCAA+AGG 0.570662 2_3:-55657516 None:intergenic
TTGGAAGGCATTGTCAGTTG+GGG 0.572321 2_3:-55657085 None:intergenic
TTGGTGAGTATGTGGAGAGG+TGG 0.574461 2_3:-55656781 None:intergenic
GGCTGTTTATCAATTGGACA+AGG 0.577680 2_3:+55657521 Msa0300310:CDS
ATTGAGGAGACGAATGAGGA+AGG 0.579677 2_3:-55656995 None:intergenic
TGTAGTGGTGAGTGCCATGT+TGG 0.588720 2_3:-55656842 None:intergenic
GAACTTTCTGTTGGTCTGAG+AGG 0.589738 2_3:-55657299 None:intergenic
GTATGTGGAGAGGTGGAGAG+GGG 0.592940 2_3:-55656774 None:intergenic
AGTGCAGCTGGAAGGAATGT+TGG 0.593131 2_3:+55657684 Msa0300310:CDS
TGTGATGTTTATTGGGCCTG+AGG 0.596385 2_3:-55656944 None:intergenic
AGTATAAATAAGTTTCACAT+TGG 0.607356 2_3:+55657929 Msa0300310:three_prime_UTR
CCATGTTGGTAAGTTAGTGA+AGG 0.621537 2_3:-55656828 None:intergenic
TCTGGTAAAGAAGGTAATTG+GGG 0.623440 2_3:+55657405 Msa0300310:CDS
CCTTCACTAACTTACCAACA+TGG 0.626947 2_3:+55656828 Msa0300310:exon
AGGATGATAGTGCAGCTGGA+AGG 0.628901 2_3:+55657676 Msa0300310:CDS
TGAGTTGTTGAGTTTCATTG+AGG 0.637415 2_3:-55657011 None:intergenic
GAGTGCAACAGGTGTTGTAG+TGG 0.647059 2_3:-55656857 None:intergenic
GGTTTGGTGAGTATGTGGAG+AGG 0.756503 2_3:-55656784 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATTAATAAGGTAATT+TGG - chr2_3:55656738-55656757 None:intergenic 10.0%
!! ATATTATTAATAAGGTAATT+TGG - chr2_3:55656738-55656757 None:intergenic 10.0%
!! TTTATATATATAATACCTGA+TGG - chr2_3:55657117-55657136 None:intergenic 15.0%
!! TTTATATATATAATACCTGA+TGG - chr2_3:55657117-55657136 None:intergenic 15.0%
!! TACATGCAAGTTATAATTAT+AGG - chr2_3:55657229-55657248 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG + chr2_3:55657264-55657283 Msa0300310:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG + chr2_3:55657929-55657948 Msa0300310:three_prime_UTR 20.0%
!! TACATGCAAGTTATAATTAT+AGG - chr2_3:55657229-55657248 None:intergenic 20.0%
!! TAACATTTATGTCTATTATC+AGG + chr2_3:55657264-55657283 Msa0300310:intron 20.0%
!! AGTATAAATAAGTTTCACAT+TGG + chr2_3:55657929-55657948 Msa0300310:three_prime_UTR 20.0%
! ATGATAGGAAATTACATGTT+GGG - chr2_3:55656586-55656605 None:intergenic 25.0%
! AATGATAGGAAATTACATGT+TGG - chr2_3:55656587-55656606 None:intergenic 25.0%
! AATGAAGTTTAACATTTGGT+TGG - chr2_3:55656610-55656629 None:intergenic 25.0%
! AAGGAATGAAGTTTAACATT+TGG - chr2_3:55656614-55656633 None:intergenic 25.0%
! TTGTATTCATTTGTCAACTA+AGG + chr2_3:55656659-55656678 Msa0300310:five_prime_UTR 25.0%
! TGTATTCATTTGTCAACTAA+GGG + chr2_3:55656660-55656679 Msa0300310:five_prime_UTR 25.0%
! TAATTTGGTTGAGAATTTGT+TGG - chr2_3:55656723-55656742 None:intergenic 25.0%
!! GTTTGAAACTGTTAAGAAAT+CGG - chr2_3:55657367-55657386 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG - chr2_3:55657834-55657853 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG + chr2_3:55657884-55657903 Msa0300310:three_prime_UTR 25.0%
! ATGATAGGAAATTACATGTT+GGG - chr2_3:55656586-55656605 None:intergenic 25.0%
! AATGATAGGAAATTACATGT+TGG - chr2_3:55656587-55656606 None:intergenic 25.0%
! AATGAAGTTTAACATTTGGT+TGG - chr2_3:55656610-55656629 None:intergenic 25.0%
! AAGGAATGAAGTTTAACATT+TGG - chr2_3:55656614-55656633 None:intergenic 25.0%
! TTGTATTCATTTGTCAACTA+AGG + chr2_3:55656659-55656678 Msa0300310:five_prime_UTR 25.0%
! TGTATTCATTTGTCAACTAA+GGG + chr2_3:55656660-55656679 Msa0300310:five_prime_UTR 25.0%
! TAATTTGGTTGAGAATTTGT+TGG - chr2_3:55656723-55656742 None:intergenic 25.0%
!! GTTTGAAACTGTTAAGAAAT+CGG - chr2_3:55657367-55657386 None:intergenic 25.0%
! ATAAATACAAAGAATAAGCC+AGG - chr2_3:55657834-55657853 None:intergenic 25.0%
!!! GATTGTCTTACATATTTTGA+AGG + chr2_3:55657884-55657903 Msa0300310:three_prime_UTR 25.0%
!! TTTTGGTTGGTTGCATTATA+TGG - chr2_3:55656525-55656544 None:intergenic 30.0%
!!! AAGTCAAGCCTAATTTGTTT+TGG - chr2_3:55656542-55656561 None:intergenic 30.0%
TAACATTTGGTTGGAATGAT+AGG - chr2_3:55656601-55656620 None:intergenic 30.0%
GGGGAGGAATATTATTAATA+AGG - chr2_3:55656746-55656765 None:intergenic 30.0%
AGAATTAAGATTGGAAGATG+AGG - chr2_3:55656808-55656827 None:intergenic 30.0%
TAGTGAAGGAGAATTAAGAT+TGG - chr2_3:55656817-55656836 None:intergenic 30.0%
AGATTGCTGTGATGTTTATT+GGG - chr2_3:55656954-55656973 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG - chr2_3:55656955-55656974 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG - chr2_3:55657107-55657126 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG - chr2_3:55657338-55657357 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG + chr2_3:55657404-55657423 Msa0300310:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG + chr2_3:55657500-55657519 Msa0300310:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG + chr2_3:55657546-55657565 Msa0300310:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG + chr2_3:55657791-55657810 Msa0300310:three_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG + chr2_3:55657792-55657811 Msa0300310:three_prime_UTR 30.0%
!! TTTTGGTTGGTTGCATTATA+TGG - chr2_3:55656525-55656544 None:intergenic 30.0%
!!! AAGTCAAGCCTAATTTGTTT+TGG - chr2_3:55656542-55656561 None:intergenic 30.0%
TAACATTTGGTTGGAATGAT+AGG - chr2_3:55656601-55656620 None:intergenic 30.0%
GGGGAGGAATATTATTAATA+AGG - chr2_3:55656746-55656765 None:intergenic 30.0%
AGAATTAAGATTGGAAGATG+AGG - chr2_3:55656808-55656827 None:intergenic 30.0%
TAGTGAAGGAGAATTAAGAT+TGG - chr2_3:55656817-55656836 None:intergenic 30.0%
AGATTGCTGTGATGTTTATT+GGG - chr2_3:55656954-55656973 None:intergenic 30.0%
AAGATTGCTGTGATGTTTAT+TGG - chr2_3:55656955-55656974 None:intergenic 30.0%
! TAATACCTGATGGAAGATTT+TGG - chr2_3:55657107-55657126 None:intergenic 30.0%
!!! AGTAGTATTTTGGTGTAACA+TGG - chr2_3:55657338-55657357 None:intergenic 30.0%
TTCTGGTAAAGAAGGTAATT+GGG + chr2_3:55657404-55657423 Msa0300310:CDS 30.0%
! TTTGAGACAACAATTTCCTT+TGG + chr2_3:55657500-55657519 Msa0300310:CDS 30.0%
! TTGTTACCTTCTGAACTTTT+TGG + chr2_3:55657546-55657565 Msa0300310:CDS 30.0%
TCATTTGATCTTCTTTGTCT+TGG + chr2_3:55657791-55657810 Msa0300310:three_prime_UTR 30.0%
CATTTGATCTTCTTTGTCTT+GGG + chr2_3:55657792-55657811 Msa0300310:three_prime_UTR 30.0%
GCAACCAACCAAAACAAATT+AGG + chr2_3:55656531-55656550 Msa0300310:five_prime_UTR 35.0%
!!! CAAGCCTAATTTGTTTTGGT+TGG - chr2_3:55656538-55656557 None:intergenic 35.0%
ATCACATCGAAGTTGCAATA+AGG - chr2_3:55656633-55656652 None:intergenic 35.0%
TAAGATTGGAAGATGAGGTT+TGG - chr2_3:55656803-55656822 None:intergenic 35.0%
TGGATTTGTGGAGTTAAGAA+GGG - chr2_3:55656901-55656920 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG - chr2_3:55656902-55656921 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG + chr2_3:55656964-55656983 Msa0300310:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG - chr2_3:55657014-55657033 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG - chr2_3:55657049-55657068 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG - chr2_3:55657311-55657330 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG - chr2_3:55657348-55657367 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG + chr2_3:55657403-55657422 Msa0300310:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG + chr2_3:55657405-55657424 Msa0300310:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG - chr2_3:55657445-55657464 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG - chr2_3:55657446-55657465 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG + chr2_3:55657450-55657469 Msa0300310:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG - chr2_3:55657489-55657508 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG + chr2_3:55657515-55657534 Msa0300310:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG - chr2_3:55657519-55657538 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG - chr2_3:55657555-55657574 None:intergenic 35.0%
!! GGTTTGATTTGATATGAACC+TGG + chr2_3:55657813-55657832 Msa0300310:three_prime_UTR 35.0%
GCAACCAACCAAAACAAATT+AGG + chr2_3:55656531-55656550 Msa0300310:five_prime_UTR 35.0%
!!! CAAGCCTAATTTGTTTTGGT+TGG - chr2_3:55656538-55656557 None:intergenic 35.0%
ATCACATCGAAGTTGCAATA+AGG - chr2_3:55656633-55656652 None:intergenic 35.0%
TAAGATTGGAAGATGAGGTT+TGG - chr2_3:55656803-55656822 None:intergenic 35.0%
TGGATTTGTGGAGTTAAGAA+GGG - chr2_3:55656901-55656920 None:intergenic 35.0%
ATGGATTTGTGGAGTTAAGA+AGG - chr2_3:55656902-55656921 None:intergenic 35.0%
! ACAGCAATCTTTGAAAAAGC+TGG + chr2_3:55656964-55656983 Msa0300310:CDS 35.0%
TGAGTTGTTGAGTTTCATTG+AGG - chr2_3:55657014-55657033 None:intergenic 35.0%
!! TTCAGAAGTTGAGTTGAGTT+GGG - chr2_3:55657049-55657068 None:intergenic 35.0%
! GAATGAGTTGAACTTTCTGT+TGG - chr2_3:55657311-55657330 None:intergenic 35.0%
! TCGGAAAGAGAGTAGTATTT+TGG - chr2_3:55657348-55657367 None:intergenic 35.0%
CTTCTGGTAAAGAAGGTAAT+TGG + chr2_3:55657403-55657422 Msa0300310:CDS 35.0%
TCTGGTAAAGAAGGTAATTG+GGG + chr2_3:55657405-55657424 Msa0300310:CDS 35.0%
!! AACATTGACTTGGTTACCTT+GGG - chr2_3:55657445-55657464 None:intergenic 35.0%
!! AAACATTGACTTGGTTACCT+TGG - chr2_3:55657446-55657465 None:intergenic 35.0%
AACCAAGTCAATGTTTCAAC+TGG + chr2_3:55657450-55657469 Msa0300310:CDS 35.0%
!! TGTCTCAAAGCATTGTTGAT+AGG - chr2_3:55657489-55657508 None:intergenic 35.0%
TCCTTTGGCTGTTTATCAAT+TGG + chr2_3:55657515-55657534 Msa0300310:CDS 35.0%
TCCAATTGATAAACAGCCAA+AGG - chr2_3:55657519-55657538 None:intergenic 35.0%
TTAGCACCAAAAAGTTCAGA+AGG - chr2_3:55657555-55657574 None:intergenic 35.0%
!! GGTTTGATTTGATATGAACC+TGG + chr2_3:55657813-55657832 Msa0300310:three_prime_UTR 35.0%
! CCATGTTGGTAAGTTAGTGA+AGG - chr2_3:55656831-55656850 None:intergenic 40.0%
CCTTCACTAACTTACCAACA+TGG + chr2_3:55656828-55656847 Msa0300310:exon 40.0%
TTTCATTGAGGAGACGAATG+AGG - chr2_3:55657002-55657021 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG - chr2_3:55657044-55657063 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG - chr2_3:55657050-55657069 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG + chr2_3:55657048-55657067 Msa0300310:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG - chr2_3:55657089-55657108 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG - chr2_3:55657090-55657109 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG - chr2_3:55657103-55657122 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG - chr2_3:55657389-55657408 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG + chr2_3:55657396-55657415 Msa0300310:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG - chr2_3:55657455-55657474 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG + chr2_3:55657521-55657540 Msa0300310:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG + chr2_3:55657572-55657591 Msa0300310:CDS 40.0%
TTCTTACTAGAAGCTGGAGA+TGG - chr2_3:55657657-55657676 None:intergenic 40.0%
ATCTCCAGCTTCTAGTAAGA+AGG + chr2_3:55657656-55657675 Msa0300310:CDS 40.0%
TCATCCTTCTTACTAGAAGC+TGG - chr2_3:55657663-55657682 None:intergenic 40.0%
!! TGTTGGTTTGGATTTGTTGC+TGG + chr2_3:55657701-55657720 Msa0300310:CDS 40.0%
!! GTTGGTTTGGATTTGTTGCT+GGG + chr2_3:55657702-55657721 Msa0300310:CDS 40.0%
! CCATGTTGGTAAGTTAGTGA+AGG - chr2_3:55656831-55656850 None:intergenic 40.0%
CCTTCACTAACTTACCAACA+TGG + chr2_3:55656828-55656847 Msa0300310:exon 40.0%
TTTCATTGAGGAGACGAATG+AGG - chr2_3:55657002-55657021 None:intergenic 40.0%
!! AAGTTGAGTTGAGTTGGGTT+TGG - chr2_3:55657044-55657063 None:intergenic 40.0%
!! CTTCAGAAGTTGAGTTGAGT+TGG - chr2_3:55657050-55657069 None:intergenic 40.0%
CAACTCAACTCAACTTCTGA+AGG + chr2_3:55657048-55657067 Msa0300310:CDS 40.0%
!! TTTGGAAGGCATTGTCAGTT+GGG - chr2_3:55657089-55657108 None:intergenic 40.0%
!!! TTTTGGAAGGCATTGTCAGT+TGG - chr2_3:55657090-55657109 None:intergenic 40.0%
! ACCTGATGGAAGATTTTGGA+AGG - chr2_3:55657103-55657122 None:intergenic 40.0%
CAGAAGCTTGTGTTCTAACT+GGG - chr2_3:55657389-55657408 None:intergenic 40.0%
ACACAAGCTTCTGGTAAAGA+AGG + chr2_3:55657396-55657415 Msa0300310:CDS 40.0%
! CTCCAGTTGAAACATTGACT+TGG - chr2_3:55657455-55657474 None:intergenic 40.0%
GGCTGTTTATCAATTGGACA+AGG + chr2_3:55657521-55657540 Msa0300310:CDS 40.0%
TAAGTCTCCATCTTCATCTC+CGG + chr2_3:55657572-55657591 Msa0300310:CDS 40.0%
TTCTTACTAGAAGCTGGAGA+TGG - chr2_3:55657657-55657676 None:intergenic 40.0%
ATCTCCAGCTTCTAGTAAGA+AGG + chr2_3:55657656-55657675 Msa0300310:CDS 40.0%
TCATCCTTCTTACTAGAAGC+TGG - chr2_3:55657663-55657682 None:intergenic 40.0%
!! TGTTGGTTTGGATTTGTTGC+TGG + chr2_3:55657701-55657720 Msa0300310:CDS 40.0%
!! GTTGGTTTGGATTTGTTGCT+GGG + chr2_3:55657702-55657721 Msa0300310:CDS 40.0%
!! GAGAATTTGTTGGCATGAGC+TGG - chr2_3:55656713-55656732 None:intergenic 45.0%
!!! GTGGAGAGGGGTTTTATTTG+GGG - chr2_3:55656765-55656784 None:intergenic 45.0%
!!! GGTGGAGAGGGGTTTTATTT+GGG - chr2_3:55656766-55656785 None:intergenic 45.0%
!!! AGGTGGAGAGGGGTTTTATT+TGG - chr2_3:55656767-55656786 None:intergenic 45.0%
!! GATGAGGTTTGGTGAGTATG+TGG - chr2_3:55656792-55656811 None:intergenic 45.0%
GTGAGAAGAATGAGTGCAAC+AGG - chr2_3:55656871-55656890 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG - chr2_3:55656946-55656965 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG - chr2_3:55656947-55656966 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG - chr2_3:55656998-55657017 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG - chr2_3:55657088-55657107 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG + chr2_3:55657099-55657118 Msa0300310:CDS 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG - chr2_3:55657302-55657321 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG - chr2_3:55657390-55657409 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG + chr2_3:55657387-55657406 Msa0300310:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG - chr2_3:55657600-55657619 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG + chr2_3:55657672-55657691 Msa0300310:CDS 45.0%
! AGCTGGAAGGAATGTTGGTT+TGG + chr2_3:55657689-55657708 Msa0300310:CDS 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG + chr2_3:55657707-55657726 Msa0300310:CDS 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG + chr2_3:55657721-55657740 Msa0300310:exon 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG + chr2_3:55657722-55657741 Msa0300310:exon 45.0%
!! GAGAATTTGTTGGCATGAGC+TGG - chr2_3:55656713-55656732 None:intergenic 45.0%
!!! GTGGAGAGGGGTTTTATTTG+GGG - chr2_3:55656765-55656784 None:intergenic 45.0%
!!! GGTGGAGAGGGGTTTTATTT+GGG - chr2_3:55656766-55656785 None:intergenic 45.0%
!!! AGGTGGAGAGGGGTTTTATT+TGG - chr2_3:55656767-55656786 None:intergenic 45.0%
!! GATGAGGTTTGGTGAGTATG+TGG - chr2_3:55656792-55656811 None:intergenic 45.0%
GTGAGAAGAATGAGTGCAAC+AGG - chr2_3:55656871-55656890 None:intergenic 45.0%
GTGATGTTTATTGGGCCTGA+GGG - chr2_3:55656946-55656965 None:intergenic 45.0%
TGTGATGTTTATTGGGCCTG+AGG - chr2_3:55656947-55656966 None:intergenic 45.0%
ATTGAGGAGACGAATGAGGA+AGG - chr2_3:55656998-55657017 None:intergenic 45.0%
!! TTGGAAGGCATTGTCAGTTG+GGG - chr2_3:55657088-55657107 None:intergenic 45.0%
GCCTTCCAAAATCTTCCATC+AGG + chr2_3:55657099-55657118 Msa0300310:CDS 45.0%
! GAACTTTCTGTTGGTCTGAG+AGG - chr2_3:55657302-55657321 None:intergenic 45.0%
CCAGAAGCTTGTGTTCTAAC+TGG - chr2_3:55657390-55657409 None:intergenic 45.0%
CCAGTTAGAACACAAGCTTC+TGG + chr2_3:55657387-55657406 Msa0300310:CDS 45.0%
! GAAGGAGTTTTCGACGACTT+AGG - chr2_3:55657600-55657619 None:intergenic 45.0%
! AAGAAGGATGATAGTGCAGC+TGG + chr2_3:55657672-55657691 Msa0300310:CDS 45.0%
! AGCTGGAAGGAATGTTGGTT+TGG + chr2_3:55657689-55657708 Msa0300310:CDS 45.0%
!! TTTGGATTTGTTGCTGGGCT+TGG + chr2_3:55657707-55657726 Msa0300310:CDS 45.0%
!! TGGGCTTGGTTTGATTTGCA+TGG + chr2_3:55657721-55657740 Msa0300310:exon 45.0%
!! GGGCTTGGTTTGATTTGCAT+GGG + chr2_3:55657722-55657741 Msa0300310:exon 45.0%
!!! ATAGTAATTTTTTTTATTAA+AGG - chr2_3:55657955-55657974 None:intergenic 5.0%
!!! ATAGTAATTTTTTTTATTAA+AGG - chr2_3:55657955-55657974 None:intergenic 5.0%
!!! GAGAGGGGTTTTATTTGGGG+AGG - chr2_3:55656762-55656781 None:intergenic 50.0%
AGTATGTGGAGAGGTGGAGA+GGG - chr2_3:55656778-55656797 None:intergenic 50.0%
TTGGTGAGTATGTGGAGAGG+TGG - chr2_3:55656784-55656803 None:intergenic 50.0%
!! GGTTTGGTGAGTATGTGGAG+AGG - chr2_3:55656787-55656806 None:intergenic 50.0%
TGTAGTGGTGAGTGCCATGT+TGG - chr2_3:55656845-55656864 None:intergenic 50.0%
! GAGTGCAACAGGTGTTGTAG+TGG - chr2_3:55656860-55656879 None:intergenic 50.0%
GGTCTTCACTTCACTTCCCA+AGG + chr2_3:55657426-55657445 Msa0300310:CDS 50.0%
TTAGGAGCCGGAGATGAAGA+TGG - chr2_3:55657582-55657601 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG - chr2_3:55657594-55657613 None:intergenic 50.0%
!!! TCGCTGCTCGGAGTTTTAGA+AGG - chr2_3:55657618-55657637 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG + chr2_3:55657676-55657695 Msa0300310:CDS 50.0%
AGTGCAGCTGGAAGGAATGT+TGG + chr2_3:55657684-55657703 Msa0300310:CDS 50.0%
!!! GAGAGGGGTTTTATTTGGGG+AGG - chr2_3:55656762-55656781 None:intergenic 50.0%
AGTATGTGGAGAGGTGGAGA+GGG - chr2_3:55656778-55656797 None:intergenic 50.0%
TTGGTGAGTATGTGGAGAGG+TGG - chr2_3:55656784-55656803 None:intergenic 50.0%
!! GGTTTGGTGAGTATGTGGAG+AGG - chr2_3:55656787-55656806 None:intergenic 50.0%
TGTAGTGGTGAGTGCCATGT+TGG - chr2_3:55656845-55656864 None:intergenic 50.0%
! GAGTGCAACAGGTGTTGTAG+TGG - chr2_3:55656860-55656879 None:intergenic 50.0%
GGTCTTCACTTCACTTCCCA+AGG + chr2_3:55657426-55657445 Msa0300310:CDS 50.0%
TTAGGAGCCGGAGATGAAGA+TGG - chr2_3:55657582-55657601 None:intergenic 50.0%
! GTTTTCGACGACTTAGGAGC+CGG - chr2_3:55657594-55657613 None:intergenic 50.0%
!!! TCGCTGCTCGGAGTTTTAGA+AGG - chr2_3:55657618-55657637 None:intergenic 50.0%
! AGGATGATAGTGCAGCTGGA+AGG + chr2_3:55657676-55657695 Msa0300310:CDS 50.0%
AGTGCAGCTGGAAGGAATGT+TGG + chr2_3:55657684-55657703 Msa0300310:CDS 50.0%
GTATGTGGAGAGGTGGAGAG+GGG - chr2_3:55656777-55656796 None:intergenic 55.0%
GAGTATGTGGAGAGGTGGAG+AGG - chr2_3:55656779-55656798 None:intergenic 55.0%
! TTTATTGGGCCTGAGGGTGC+TGG - chr2_3:55656940-55656959 None:intergenic 55.0%
TCACCTTCAACGTCGCTGCT+CGG - chr2_3:55657630-55657649 None:intergenic 55.0%
GTATGTGGAGAGGTGGAGAG+GGG - chr2_3:55656777-55656796 None:intergenic 55.0%
GAGTATGTGGAGAGGTGGAG+AGG - chr2_3:55656779-55656798 None:intergenic 55.0%
! TTTATTGGGCCTGAGGGTGC+TGG - chr2_3:55656940-55656959 None:intergenic 55.0%
TCACCTTCAACGTCGCTGCT+CGG - chr2_3:55657630-55657649 None:intergenic 55.0%
GGGGCTTGGGCATGGATTTG+TGG - chr2_3:55656913-55656932 None:intergenic 60.0%
ACTCCGAGCAGCGACGTTGA+AGG + chr2_3:55657624-55657643 Msa0300310:CDS 60.0%
GGGGCTTGGGCATGGATTTG+TGG - chr2_3:55656913-55656932 None:intergenic 60.0%
ACTCCGAGCAGCGACGTTGA+AGG + chr2_3:55657624-55657643 Msa0300310:CDS 60.0%
! CTGGTGCTGGGGCTTGGGCA+TGG - chr2_3:55656921-55656940 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG - chr2_3:55656926-55656945 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG - chr2_3:55656927-55656946 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG - chr2_3:55656932-55656951 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG - chr2_3:55656933-55656952 None:intergenic 70.0%
! CTGGTGCTGGGGCTTGGGCA+TGG - chr2_3:55656921-55656940 None:intergenic 70.0%
!! GGGTGCTGGTGCTGGGGCTT+GGG - chr2_3:55656926-55656945 None:intergenic 70.0%
!! AGGGTGCTGGTGCTGGGGCT+TGG - chr2_3:55656927-55656946 None:intergenic 70.0%
!! GCCTGAGGGTGCTGGTGCTG+GGG - chr2_3:55656932-55656951 None:intergenic 70.0%
!! GGCCTGAGGGTGCTGGTGCT+GGG - chr2_3:55656933-55656952 None:intergenic 70.0%
GCCCCAGCACCAGCACCCTC+AGG + chr2_3:55656928-55656947 Msa0300310:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG - chr2_3:55656934-55656953 None:intergenic 75.0%
GCCCCAGCACCAGCACCCTC+AGG + chr2_3:55656928-55656947 Msa0300310:CDS 75.0%
!! GGGCCTGAGGGTGCTGGTGC+TGG - chr2_3:55656934-55656953 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55656517 55657982 55656517 ID=Msa0300310;Name=Msa0300310
chr2_3 mRNA 55656517 55657982 55656517 ID=Msa0300310-mRNA-1;Parent=Msa0300310;Name=Msa0300310-mRNA-1;_AED=0.00;_eAED=0.00;_QI=330|1|1|1|1|1|2|248|240
chr2_3 exon 55656517 55657120 55656517 ID=Msa0300310-mRNA-1:exon:17771;Parent=Msa0300310-mRNA-1
chr2_3 exon 55657286 55657982 55657286 ID=Msa0300310-mRNA-1:exon:17772;Parent=Msa0300310-mRNA-1
chr2_3 five_prime_UTR 55656517 55656846 55656517 ID=Msa0300310-mRNA-1:five_prime_utr;Parent=Msa0300310-mRNA-1
chr2_3 CDS 55656847 55657120 55656847 ID=Msa0300310-mRNA-1:cds;Parent=Msa0300310-mRNA-1
chr2_3 CDS 55657286 55657734 55657286 ID=Msa0300310-mRNA-1:cds;Parent=Msa0300310-mRNA-1
chr2_3 three_prime_UTR 55657735 55657982 55657735 ID=Msa0300310-mRNA-1:three_prime_utr;Parent=Msa0300310-mRNA-1
Gene Sequence

>Msa0300310

ATGGCACTCACCACTACAACACCTGTTGCACTCATTCTTCTCACTCTCATTCCCTTCTTAACTCCACAAATCCATGCCCAAGCCCCAGCACCAGCACCCTCAGGCCCAATAAACATCACAGCAATCTTTGAAAAAGCTGGTCAATACACCTTCCTCATTCGTCTCCTCAATGAAACTCAACAACTCACTCAAATCCAAACCCAACTCAACTCAACTTCTGAAGGTTTCACAATCTTTGCCCCAACTGACAATGCCTTCCAAAATCTTCCATCAGGAGCAATCAACGACCTCTCAGACCAACAGAAAGTTCAACTCATTCTCTACCATGTTACACCAAAATACTACTCTCTTTCCGATTTCTTAACAGTTTCAAACCCAGTTAGAACACAAGCTTCTGGTAAAGAAGGTAATTGGGGTCTTCACTTCACTTCCCAAGGTAACCAAGTCAATGTTTCAACTGGAGTTGTTACTGTACCTATCAACAATGCTTTGAGACAACAATTTCCTTTGGCTGTTTATCAATTGGACAAGGTTTTGTTACCTTCTGAACTTTTTGGTGCTAAGTCTCCATCTTCATCTCCGGCTCCTAAGTCGTCGAAAACTCCTTCTAAAACTCCGAGCAGCGACGTTGAAGGTGATGCTCCATCTCCAGCTTCTAGTAAGAAGGATGATAGTGCAGCTGGAAGGAATGTTGGTTTGGATTTGTTGCTGGGCTTGGTTTGA
Protein sequence

>Msa0300310

MALTTTTPVALILLTLIPFLTPQIHAQAPAPAPSGPINITAIFEKAGQYTFLIRLLNETQQLTQIQTQLNSTSEGFTIFAPTDNAFQNLPSGAINDLSDQQKVQLILYHVTPKYYSLSDFLTVSNPVRTQASGKEGNWGLHFTSQGNQVNVSTGVVTVPINNALRQQFPLAVYQLDKVLLPSELFGAKSPSSSPAPKSSKTPSKTPSSDVEGDAPSPASSKKDDSAAGRNVGLDLLLGLV*