AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0357680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTACTCCTACCAGCATTCT+TGG 0.203064 3_1:+41189204 None:intergenic
TTGCTTCATGTCATCTGTTT+TGG 0.221742 3_1:-41189231 Msa0357680:CDS
GTCTTCAGCACCTTCTTCTT+TGG 0.261686 3_1:+41191184 None:intergenic
CAAAATTTCTGATTGGCTTT+CGG 0.295091 3_1:-41191360 None:intergenic
GAGCAAACCTTTCACCGTTC+TGG 0.303491 3_1:+41191278 None:intergenic
AAAGGACAGTTGAAAATAAG+AGG 0.395303 3_1:-41191136 Msa0357680:CDS
GATTGGCTTTCGGTGGGAAC+AGG 0.442402 3_1:-41191350 Msa0357680:CDS
AGGAAGCGCAGCAACGATTC+CGG 0.449386 3_1:-41191330 Msa0357680:CDS
AAGCATGAATCGATACATAA+AGG 0.452152 3_1:-41191154 Msa0357680:CDS
ACTGGAAAACCTTCTGCAAA+CGG 0.491750 3_1:+41189256 None:intergenic
AATTTCTGATTGGCTTTCGG+TGG 0.517763 3_1:-41191357 None:intergenic
GTTGTTGATCCGTTTGCAGA+AGG 0.523300 3_1:-41189265 Msa0357680:CDS
GTTGCAGCTTCCAAAGAAGA+AGG 0.528880 3_1:-41191194 Msa0357680:intron
ATTTATTGATTGCAGGGACA+AGG 0.544031 3_1:-41188845 Msa0357680:intron
AGATGACATGAAGCAATGAC+TGG 0.561776 3_1:+41189238 None:intergenic
CCAAGAATGCTGGTAGGAGT+AGG 0.574609 3_1:-41189204 Msa0357680:intron
GGAAGAATTATCTTCGCCGC+CGG 0.577024 3_1:+41191311 None:intergenic
ATTTCTGATTGGCTTTCGGT+GGG 0.581141 3_1:-41191356 None:intergenic
CTGCAAGTTTGATGATGAAG+AGG 0.584355 3_1:-41191096 Msa0357680:intron
AAAAGCAACATGGACACCAT+AGG 0.595275 3_1:+41188986 None:intergenic
AAGGACAGTTGAAAATAAGA+GGG 0.618390 3_1:-41191135 Msa0357680:CDS
CATGAACCTTAAAAGCAACA+TGG 0.619303 3_1:+41188976 None:intergenic
AAGCGCAGCAACGATTCCGG+CGG 0.644728 3_1:-41191327 Msa0357680:CDS
GGATAACCAAGAATGCTGGT+AGG 0.645233 3_1:-41189210 Msa0357680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGAATTTTTTTCTATATAT+AGG - chr3_1:41190603-41190622 Msa0357680:intron 10.0%
!! AAAACAAATAAAATTAAGAA+CGG + chr3_1:41191285-41191304 None:intergenic 10.0%
!!! TTTCGTAAATTTTGATAATT+TGG - chr3_1:41189861-41189880 Msa0357680:intron 15.0%
!!! TCTATCTATTTTTGATAATT+TGG - chr3_1:41189930-41189949 Msa0357680:intron 15.0%
!! TAGATCAAGTTTAAATTAAT+TGG + chr3_1:41190227-41190246 None:intergenic 15.0%
!! AAAAGCAATTAACATTAATA+TGG + chr3_1:41190330-41190349 None:intergenic 15.0%
!!! TGTTTTATGGTTTAAATTAA+TGG - chr3_1:41190675-41190694 Msa0357680:intron 15.0%
!! AACTCTATAAAATAATCTAA+AGG + chr3_1:41191065-41191084 None:intergenic 15.0%
!!! TATTTTATAGAGTTATTTTC+TGG - chr3_1:41191071-41191090 Msa0357680:intron 15.0%
!!! AAGAAATTTTTTGAAACAAT+TGG + chr3_1:41191311-41191330 None:intergenic 15.0%
!!! TTGTTGTTATTTTGATTGTT+TGG - chr3_1:41188945-41188964 Msa0357680:intron 20.0%
!! AATCTAATCATACTCAAATT+AGG + chr3_1:41189144-41189163 None:intergenic 20.0%
!!! CTTTTTCAACTCAAAATTAT+TGG - chr3_1:41189215-41189234 Msa0357680:CDS 20.0%
!!! TACTTTGTTTTTGTTACTTA+TGG - chr3_1:41189370-41189389 Msa0357680:intron 20.0%
!! AGCATGAAAAAACATATATA+AGG + chr3_1:41190039-41190058 None:intergenic 20.0%
!! ACATATGTCTACAAAAATAT+TGG + chr3_1:41190069-41190088 None:intergenic 20.0%
!!! ACTCTAGAATTTAATATAGA+AGG + chr3_1:41190194-41190213 None:intergenic 20.0%
!!! TTTATGAATTAAGCTTTGTT+TGG - chr3_1:41190884-41190903 Msa0357680:intron 20.0%
!!! TTTTAGTTGTTTGTACATAA+TGG - chr3_1:41191013-41191032 Msa0357680:intron 20.0%
! GAAAAAGTTCAGATACATAA+AGG + chr3_1:41189202-41189221 None:intergenic 25.0%
! TGATGACTAATCTAGAAAAT+GGG + chr3_1:41189267-41189286 None:intergenic 25.0%
!! TTGATGACTAATCTAGAAAA+TGG + chr3_1:41189268-41189287 None:intergenic 25.0%
! GTAGAAGATATAACTAACAA+AGG - chr3_1:41189458-41189477 Msa0357680:intron 25.0%
! AAACTAATTCCGACAAAATT+GGG + chr3_1:41189487-41189506 None:intergenic 25.0%
! AAAACTAATTCCGACAAAAT+TGG + chr3_1:41189488-41189507 None:intergenic 25.0%
!! AATGAAGCATTATAACAAAC+AGG + chr3_1:41189542-41189561 None:intergenic 25.0%
! GTAGCTTAATCAAGATTATA+AGG + chr3_1:41189742-41189761 None:intergenic 25.0%
!! AAACTCATCACAACATTTTT+AGG + chr3_1:41189764-41189783 None:intergenic 25.0%
! TTGATTAGAGGACAAAATAT+TGG + chr3_1:41189978-41189997 None:intergenic 25.0%
!!! CTAAATTTGTTTTCATCCAT+TGG - chr3_1:41190119-41190138 Msa0357680:intron 25.0%
! AAACTTGATCTAATACATCA+TGG - chr3_1:41190235-41190254 Msa0357680:intron 25.0%
!! AGTGCTAATTTACTGTTTTA+TGG - chr3_1:41190662-41190681 Msa0357680:intron 25.0%
! ATTCAAACGATATCAAAACA+GGG + chr3_1:41190714-41190733 None:intergenic 25.0%
! AATTCAAACGATATCAAAAC+AGG + chr3_1:41190715-41190734 None:intergenic 25.0%
! CAACTTATAAAATCTGATGT+TGG - chr3_1:41190771-41190790 Msa0357680:intron 25.0%
! GTTATGAAAGATGTTGAATA+GGG - chr3_1:41190793-41190812 Msa0357680:intron 25.0%
AAGCATGAATCGATACATAA+AGG - chr3_1:41189031-41189050 Msa0357680:CDS 30.0%
AAAGGACAGTTGAAAATAAG+AGG - chr3_1:41189049-41189068 Msa0357680:CDS 30.0%
AAGGACAGTTGAAAATAAGA+GGG - chr3_1:41189050-41189069 Msa0357680:CDS 30.0%
!!! TTTGGTTTTTGTTTCAGCTT+TGG - chr3_1:41189400-41189419 Msa0357680:intron 30.0%
GTTATATCTTCTACATCTTG+TGG + chr3_1:41189452-41189471 None:intergenic 30.0%
AAGCATTATAACAAACAGGT+GGG + chr3_1:41189538-41189557 None:intergenic 30.0%
GTTTCATCATTTATCTTCCA+CGG - chr3_1:41189626-41189645 Msa0357680:intron 30.0%
ATAACTTTAAACCTAAACCG+TGG + chr3_1:41189646-41189665 None:intergenic 30.0%
! TGATAATTTGGTGACTTACA+TGG - chr3_1:41189873-41189892 Msa0357680:intron 30.0%
! GATAATTTGGTGACTTACAT+GGG - chr3_1:41189874-41189893 Msa0357680:intron 30.0%
TACATGGGAGATTCTATAAT+AGG - chr3_1:41189889-41189908 Msa0357680:intron 30.0%
GCATCAGAAAATTTGATTAG+AGG + chr3_1:41189990-41190009 None:intergenic 30.0%
CAAGTACTTTAGACTTCAAA+AGG + chr3_1:41190432-41190451 None:intergenic 30.0%
! TGAAGTCTAAAGTACTTGAT+TGG - chr3_1:41190434-41190453 Msa0357680:intron 30.0%
AGATTGCATATAAAAGACAC+CGG + chr3_1:41190638-41190657 None:intergenic 30.0%
GATTATAATCTCAGTTTCCA+CGG + chr3_1:41190748-41190767 None:intergenic 30.0%
GGTTATGAAAGATGTTGAAT+AGG - chr3_1:41190792-41190811 Msa0357680:intron 30.0%
! TGCTGCTAATTTTACACTTT+TGG - chr3_1:41191120-41191139 Msa0357680:CDS 30.0%
! TGCTAATTTTACACTTTTGG+TGG - chr3_1:41191123-41191142 Msa0357680:CDS 30.0%
! GCTAATTTTACACTTTTGGT+GGG - chr3_1:41191124-41191143 Msa0357680:CDS 30.0%
! CTAATTTTACACTTTTGGTG+GGG - chr3_1:41191125-41191144 Msa0357680:CDS 30.0%
!! TTTTGTTTGTTCAAGTCCTA+TGG - chr3_1:41191183-41191202 Msa0357680:CDS 30.0%
!!! TGCTTTTAAGGTTCATGTAT+TGG - chr3_1:41191215-41191234 Msa0357680:intron 30.0%
ATTGTATACTGTCCAAAAAC+AGG + chr3_1:41191252-41191271 None:intergenic 30.0%
TTCCAAGAGAAAAACCAGAA+CGG - chr3_1:41188893-41188912 Msa0357680:intron 35.0%
TGAGCTATAATTATGAGACG+AGG - chr3_1:41189307-41189326 Msa0357680:intron 35.0%
TTATGAGCAAGCACAAAAAC+AGG + chr3_1:41189333-41189352 None:intergenic 35.0%
!!! GTTACTTATGGTTGCTGTTT+TGG - chr3_1:41189382-41189401 Msa0357680:intron 35.0%
TGGCAAATATTCCTAAGAAC+AGG - chr3_1:41189420-41189439 Msa0357680:intron 35.0%
TATCTTCTACATCTTGTGGT+TGG + chr3_1:41189448-41189467 None:intergenic 35.0%
! CAAAGGTAACCCAATTTTGT+CGG - chr3_1:41189475-41189494 Msa0357680:intron 35.0%
AGCATTATAACAAACAGGTG+GGG + chr3_1:41189537-41189556 None:intergenic 35.0%
! GAAGCATTATAACAAACAGG+TGG + chr3_1:41189539-41189558 None:intergenic 35.0%
TTCAACACATATTTGCCTGA+TGG - chr3_1:41189567-41189586 Msa0357680:intron 35.0%
TCATTTATCTTCCACGGTTT+AGG - chr3_1:41189632-41189651 Msa0357680:intron 35.0%
!! TCATGGTGATTTGATAATGC+AGG - chr3_1:41190252-41190271 Msa0357680:intron 35.0%
!! GTGATTTGATAATGCAGGTT+TGG - chr3_1:41190257-41190276 Msa0357680:intron 35.0%
TGTCTTTGGCCTATTCATAT+GGG - chr3_1:41190280-41190299 Msa0357680:intron 35.0%
! CACACTTTTCCCATATGAAT+AGG + chr3_1:41190292-41190311 None:intergenic 35.0%
! TTCATATGGGAAAAGTGTGA+AGG - chr3_1:41190293-41190312 Msa0357680:intron 35.0%
ATATCTTGAAGCTTTCACAC+TGG + chr3_1:41190363-41190382 None:intergenic 35.0%
TTGACCATCTCAATTTGCAT+GGG + chr3_1:41190573-41190592 None:intergenic 35.0%
ATTGACCATCTCAATTTGCA+TGG + chr3_1:41190574-41190593 None:intergenic 35.0%
TATATATAGGTGCTTCAACC+CGG - chr3_1:41190616-41190635 Msa0357680:intron 35.0%
GATTGCATATAAAAGACACC+GGG + chr3_1:41190637-41190656 None:intergenic 35.0%
! TTGCTTCATGTCATCTGTTT+TGG - chr3_1:41190954-41190973 Msa0357680:intron 35.0%
! TTTTGGATAACCAAGAATGC+TGG - chr3_1:41190971-41190990 Msa0357680:intron 35.0%
CATGAACCTTAAAAGCAACA+TGG + chr3_1:41191212-41191231 None:intergenic 35.0%
! TCCTTCCATTTATTGATTGC+AGG - chr3_1:41191333-41191352 Msa0357680:CDS 35.0%
! CCTTCCATTTATTGATTGCA+GGG - chr3_1:41191334-41191353 Msa0357680:CDS 35.0%
TTGTCCCTGCAATCAATAAA+TGG + chr3_1:41191341-41191360 None:intergenic 35.0%
! ATTTATTGATTGCAGGGACA+AGG - chr3_1:41191340-41191359 Msa0357680:CDS 35.0%
AGAAAAACCAGAACGGTGAA+AGG - chr3_1:41188900-41188919 Msa0357680:intron 40.0%
!! CTGCAAGTTTGATGATGAAG+AGG - chr3_1:41189089-41189108 Msa0357680:intron 40.0%
TATAACAAACAGGTGGGGAA+GGG + chr3_1:41189532-41189551 None:intergenic 40.0%
TTATAACAAACAGGTGGGGA+AGG + chr3_1:41189533-41189552 None:intergenic 40.0%
TTCCAAAACTCAGTTGAGTG+TGG + chr3_1:41189604-41189623 None:intergenic 40.0%
TTATATCACTCACGTGTAGC+TGG + chr3_1:41190151-41190170 None:intergenic 40.0%
!! TAATGCAGGTTTGGTGTCTT+TGG - chr3_1:41190266-41190285 Msa0357680:intron 40.0%
GTGTCTTTGGCCTATTCATA+TGG - chr3_1:41190279-41190298 Msa0357680:intron 40.0%
CATCTTGGACAATAGCTTCA+TGG + chr3_1:41190502-41190521 None:intergenic 40.0%
!! GTTTTGACAGCAACACATCT+TGG + chr3_1:41190517-41190536 None:intergenic 40.0%
TAGACCCATGCAAATTGAGA+TGG - chr3_1:41190566-41190585 Msa0357680:intron 40.0%
CAAAACAGGGATGCATGATT+TGG + chr3_1:41190701-41190720 None:intergenic 40.0%
TCTAGCAAAGGAAGGATCTT+CGG - chr3_1:41190838-41190857 Msa0357680:intron 40.0%
TGACAGGACAACATGTAGTT+TGG - chr3_1:41190861-41190880 Msa0357680:intron 40.0%
!! AAGCTTTGTTTGGTTTCTGC+AGG - chr3_1:41190894-41190913 Msa0357680:intron 40.0%
!! AGCTTTGTTTGGTTTCTGCA+GGG - chr3_1:41190895-41190914 Msa0357680:intron 40.0%
ACTGGAAAACCTTCTGCAAA+CGG + chr3_1:41190932-41190951 None:intergenic 40.0%
AGATGACATGAAGCAATGAC+TGG + chr3_1:41190950-41190969 None:intergenic 40.0%
! TTACACTTTTGGTGGGGTTT+TGG - chr3_1:41191131-41191150 Msa0357680:CDS 40.0%
AAAAGCAACATGGACACCAT+AGG + chr3_1:41191202-41191221 None:intergenic 40.0%
!!! TGGTGTCCATGTTGCTTTTA+AGG - chr3_1:41191203-41191222 Msa0357680:intron 40.0%
! GTGAGTTAGCAACCTGTTTT+TGG - chr3_1:41191237-41191256 Msa0357680:intron 40.0%
CCCTGCAATCAATAAATGGA+AGG + chr3_1:41191337-41191356 None:intergenic 40.0%
!!! CACCGTTCTGGTTTTTCTCT+TGG + chr3_1:41188898-41188917 None:intergenic 45.0%
GTTGCAGCTTCCAAAGAAGA+AGG - chr3_1:41188991-41189010 Msa0357680:CDS 45.0%
GTCTTCAGCACCTTCTTCTT+TGG + chr3_1:41189004-41189023 None:intergenic 45.0%
! TGTGGTTGGTTCCTGTTCTT+AGG + chr3_1:41189434-41189453 None:intergenic 45.0%
! TGCCACACTCAACTGAGTTT+TGG - chr3_1:41189599-41189618 Msa0357680:intron 45.0%
TTGAATTTCGTGAGAGTCCG+TGG - chr3_1:41190728-41190747 Msa0357680:intron 45.0%
GAATAGGGAAGCAAACCAAG+AGG - chr3_1:41190808-41190827 Msa0357680:intron 45.0%
TTGCTAGACAATCTGCCTCT+TGG + chr3_1:41190826-41190845 None:intergenic 45.0%
AGAGGCAGATTGTCTAGCAA+AGG - chr3_1:41190826-41190845 Msa0357680:intron 45.0%
GCAGATTGTCTAGCAAAGGA+AGG - chr3_1:41190830-41190849 Msa0357680:intron 45.0%
GTTGTTGATCCGTTTGCAGA+AGG - chr3_1:41190920-41190939 Msa0357680:intron 45.0%
GGATAACCAAGAATGCTGGT+AGG - chr3_1:41190975-41190994 Msa0357680:intron 45.0%
GGAAGAATTATCTTCGCCGC+CGG + chr3_1:41188877-41188896 None:intergenic 50.0%
GAGCAAACCTTTCACCGTTC+TGG + chr3_1:41188910-41188929 None:intergenic 50.0%
GTGGCAGAGAATCAACCATC+AGG + chr3_1:41189585-41189604 None:intergenic 50.0%
AAGGAAGGATCTTCGGTGAC+AGG - chr3_1:41190845-41190864 Msa0357680:intron 50.0%
CCTACTCCTACCAGCATTCT+TGG + chr3_1:41190984-41191003 None:intergenic 50.0%
CCAAGAATGCTGGTAGGAGT+AGG - chr3_1:41190981-41191000 Msa0357680:intron 50.0%
TTCGTCCTTTAGTCACGAGC+AGG - chr3_1:41191039-41191058 Msa0357680:intron 50.0%
AAAGGCCTGCTCGTGACTAA+AGG + chr3_1:41191047-41191066 None:intergenic 50.0%
! AGGAAGCGCAGCAACGATTC+CGG - chr3_1:41188855-41188874 Msa0357680:intron 55.0%
!! GTGTAGCTGGCTTGTGCCAA+TGG + chr3_1:41190138-41190157 None:intergenic 55.0%
AAGCGCAGCAACGATTCCGG+CGG - chr3_1:41188858-41188877 Msa0357680:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 41188837 41191370 41188837 ID=Msa0357680;Name=Msa0357680
chr3_1 mRNA 41188837 41191370 41188837 ID=Msa0357680-mRNA-1;Parent=Msa0357680;Name=Msa0357680-mRNA-1;_AED=0.63;_eAED=0.78;_QI=0|0|0.2|0.4|1|1|5|0|137
chr3_1 exon 41191293 41191370 41191293 ID=Msa0357680-mRNA-1:exon:7216;Parent=Msa0357680-mRNA-1
chr3_1 exon 41191097 41191209 41191097 ID=Msa0357680-mRNA-1:exon:7215;Parent=Msa0357680-mRNA-1
chr3_1 exon 41189205 41189291 41189205 ID=Msa0357680-mRNA-1:exon:7214;Parent=Msa0357680-mRNA-1
chr3_1 exon 41188983 41189102 41188983 ID=Msa0357680-mRNA-1:exon:7213;Parent=Msa0357680-mRNA-1
chr3_1 exon 41188837 41188852 41188837 ID=Msa0357680-mRNA-1:exon:7212;Parent=Msa0357680-mRNA-1
chr3_1 CDS 41191293 41191370 41191293 ID=Msa0357680-mRNA-1:cds;Parent=Msa0357680-mRNA-1
chr3_1 CDS 41191097 41191209 41191097 ID=Msa0357680-mRNA-1:cds;Parent=Msa0357680-mRNA-1
chr3_1 CDS 41189205 41189291 41189205 ID=Msa0357680-mRNA-1:cds;Parent=Msa0357680-mRNA-1
chr3_1 CDS 41188983 41189102 41188983 ID=Msa0357680-mRNA-1:cds;Parent=Msa0357680-mRNA-1
chr3_1 CDS 41188837 41188852 41188837 ID=Msa0357680-mRNA-1:cds;Parent=Msa0357680-mRNA-1
Gene Sequence

>Msa0357680

TTGGCTTTCGGTGGGAACAGGAAGCGCAGCAACGATTCCGGCGGCGAAGATAATTCTTCCAAGAGAAAAACCAGAACGCTTCCAAAGAAGAAGGTGCTGAAGACTATCTGCAAGCATGAATCGATACATAAAGGACAGTTGAAAATAAGAGGGAAGTTGATGAAACTATCTGCAAGTTTGATGATGAAGAGGGCTGTTGTTGATCCGTTTGCAGAAGGTTTTCCAGTCATTGCTTCATGTCATCTGTTTTGGATAACCAAGAATGCTGGTAGGAGTAGCAACATGTTATTCTATGCTGCTAATTTTACACTTTTGGTGGGGTTTTGGTGCAACAGAAAGTTCTTATTTGCTGCTATTTTTGTTTGTTCAAGTCCTATGGTGTCCATGTTGCTTTTAAGGGACAAGGACTGTTAA

Protein sequence

>Msa0357680

LAFGGNRKRSNDSGGEDNSSKRKTRTLPKKKVLKTICKHESIHKGQLKIRGKLMKLSASLMMKRAVVDPFAEGFPVIASCHLFWITKNAGRSSNMLFYAANFTLLVGFWCNRKFLFAAIFVCSSPMVSMLLLRDKDC*