AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0363320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0363320 MtrunA17_Chr3g0102051 93.158 190 8 1 36 225 35 219 1.38e-125 354
Msa0363320 MtrunA17_Chr5g0399711 58.824 102 42 0 120 221 121 222 1.88e-38 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0363320 AT5G11750.1 73.377 154 33 3 74 225 80 227 3.08e-73 222
Msa0363320 AT4G11630.1 72.848 151 33 2 75 225 81 223 2.11e-72 220
Msa0363320 AT5G11750.2 65.896 173 37 4 74 225 80 251 1.13e-70 216
Msa0363320 AT1G24240.1 71.154 156 37 2 70 225 73 220 1.19e-63 197
Msa0363320 AT4G17560.1 60.000 105 42 0 120 224 117 221 3.91e-36 127
Msa0363320 AT5G47190.1 59.804 102 41 0 120 221 121 222 1.11e-35 126
Msa0363320 AT1G24240.2 60.759 79 23 2 70 148 73 143 1.55e-21 87.4

Find 68 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCGTGTTTCTATTATAAA+AGG 0.194759 3_1:-55586955 Msa0363320:CDS
GGGTCTGGTGTTGCGGATAT+TGG 0.227977 3_1:-55587881 Msa0363320:CDS
TTGATGCGGGGAGGCAATTC+AGG 0.268789 3_1:+55587320 None:intergenic
CTTTCGATCATATCGTGTTT+TGG 0.276614 3_1:-55586027 Msa0363320:three_prime_UTR
GCGGATATTGGGTTGATTAT+TGG 0.296717 3_1:-55587869 Msa0363320:CDS
TTGTCAGGCGTCCACAGTTC+TGG 0.310894 3_1:-55587945 Msa0363320:intron
TGTGGACAGGTCTGGACTTC+AGG 0.311298 3_1:-55587918 Msa0363320:CDS
ATCCCTCAGGTAGTAGAGTT+TGG 0.318153 3_1:+55586238 None:intergenic
GGTCTGGTGTTGCGGATATT+GGG 0.355504 3_1:-55587880 Msa0363320:CDS
ACCTTTATCTCCTTTATGTT+AGG 0.356884 3_1:+55586287 None:intergenic
ACCTAACATAAAGGAGATAA+AGG 0.358575 3_1:-55586288 Msa0363320:CDS
CGGGGAGGCAATTCAGGTTC+TGG 0.369112 3_1:+55587326 None:intergenic
CTTCAGGTGCTAAGCAGAGC+TGG 0.383541 3_1:-55587902 Msa0363320:CDS
CTGGTTTGTGTGGACAGGTC+TGG 0.390687 3_1:-55587926 Msa0363320:CDS
ACACTACATTCAGACTTAGA+AGG 0.398714 3_1:-55586900 Msa0363320:CDS
GAAGGATGGTAGCTGGGGTT+GGG 0.398853 3_1:-55586882 Msa0363320:CDS
TTGTCCCTCATTTCCGAAGC+AGG 0.430695 3_1:-55587400 Msa0363320:CDS
ATATAACCAGGCTTTATATC+AGG 0.433635 3_1:+55587117 None:intergenic
AGAAGGATGGTAGCTGGGGT+TGG 0.434672 3_1:-55586883 Msa0363320:CDS
ATGCTGAAATGAAGGCAATC+TGG 0.441606 3_1:+55587433 None:intergenic
CAGTTCTGGTTTGTGTGGAC+AGG 0.444101 3_1:-55587931 Msa0363320:CDS
CGGTAGACATAAACCTGCTT+CGG 0.445201 3_1:+55587387 None:intergenic
TAATCTCTTAAATTTGATGC+GGG 0.446449 3_1:+55587307 None:intergenic
AGAATGCATGTGAATACATA+TGG 0.454243 3_1:+55586128 None:intergenic
GACTTAGAAGGATGGTAGCT+GGG 0.461287 3_1:-55586888 Msa0363320:CDS
GGCGTGTTTCTATTATAAAA+GGG 0.465947 3_1:-55586954 Msa0363320:CDS
GTGCTAAGCAGAGCTGGGTC+TGG 0.471202 3_1:-55587896 Msa0363320:CDS
AGACTTAGAAGGATGGTAGC+TGG 0.494744 3_1:-55586889 Msa0363320:CDS
AGACAAAGAAGCAGTGGAGG+AGG 0.496174 3_1:-55587160 Msa0363320:CDS
ATAAACCTGCTTCGGAAATG+AGG 0.502963 3_1:+55587395 None:intergenic
TAAAAGGGATTGTTATTGCA+AGG 0.505129 3_1:-55586939 Msa0363320:CDS
GTATCCTCTAGAGATGAGTC+TGG 0.510292 3_1:-55587467 Msa0363320:CDS
GATATTGGGTTGATTATTGG+TGG 0.510439 3_1:-55587866 Msa0363320:CDS
AACCGCGAGGCACATTATGC+AGG 0.510916 3_1:-55587279 Msa0363320:CDS
ACAGAACCATGCTGAAATGA+AGG 0.517294 3_1:+55587425 None:intergenic
CAGGTATTCACCTAACATAA+AGG 0.524817 3_1:-55586297 Msa0363320:CDS
GACACTAGTCTTCAGCCTGT+TGG 0.531116 3_1:-55586189 Msa0363320:three_prime_UTR
CAGAGCTGGGTCTGGTGTTG+CGG 0.531967 3_1:-55587888 Msa0363320:CDS
TCTAGAGATGAGTCTGGTAA+TGG 0.532054 3_1:-55587461 Msa0363320:CDS
CTAATCTCTTAAATTTGATG+CGG 0.540456 3_1:+55587306 None:intergenic
TCATATATCATAGGAAGTAC+CGG 0.546725 3_1:-55586986 Msa0363320:intron
GAGGTACCTGATATAAAGCC+TGG 0.550742 3_1:-55587123 Msa0363320:CDS
GAAACACGCCTCTTGTTCTC+CGG 0.554578 3_1:+55586967 None:intergenic
TTCAGGTGCTAAGCAGAGCT+GGG 0.555663 3_1:-55587901 Msa0363320:CDS
AGGCAATTCAGGTTCTGGTA+AGG 0.561434 3_1:+55587331 None:intergenic
CAAAACACGATATGATCGAA+AGG 0.561824 3_1:+55586028 None:intergenic
TTTAGACAAAGAAGCAGTGG+AGG 0.569964 3_1:-55587163 Msa0363320:CDS
GTTATTGCAAGGCGTAATTG+TGG 0.573207 3_1:-55586928 Msa0363320:CDS
AAAACACGATATGATCGAAA+GGG 0.580980 3_1:+55586029 None:intergenic
TAAACCTGCTTCGGAAATGA+GGG 0.601675 3_1:+55587396 None:intergenic
CTCTTAAATTTGATGCGGGG+AGG 0.603126 3_1:+55587311 None:intergenic
AGGAAGTACCGGAGAACAAG+AGG 0.604082 3_1:-55586975 Msa0363320:CDS
GGAGGTGAAAGAACAGAGAG+AGG 0.615909 3_1:-55587142 Msa0363320:CDS
GTCCACAGTTCTGGTTTGTG+TGG 0.617070 3_1:-55587936 Msa0363320:CDS
TTGAGCTGCACAATATAACC+AGG 0.624977 3_1:+55587105 None:intergenic
AGAATCAACAGAATTTGTCT+CGG 0.631893 3_1:+55587367 None:intergenic
TACATTCAGACTTAGAAGGA+TGG 0.634854 3_1:-55586896 Msa0363320:CDS
GAGCCAAACTCTACTACCTG+AGG 0.635973 3_1:-55586241 Msa0363320:CDS
AGATTGCCTTCATTTCAGCA+TGG 0.636188 3_1:-55587431 Msa0363320:CDS
ACTTAGAAGGATGGTAGCTG+GGG 0.639779 3_1:-55586887 Msa0363320:CDS
CTAGAGGATACATTCGTCTG+AGG 0.640083 3_1:+55587479 None:intergenic
AATCTCTTAAATTTGATGCG+GGG 0.640370 3_1:+55587308 None:intergenic
ATTACCAGACTCATCTCTAG+AGG 0.648515 3_1:+55587463 None:intergenic
GTCCACACAAACCAGAACTG+TGG 0.675331 3_1:+55587934 None:intergenic
AGCCAAACTCTACTACCTGA+GGG 0.676552 3_1:-55586240 Msa0363320:CDS
TTATATTGTGCAGCTCAAAG+TGG 0.710180 3_1:-55587100 Msa0363320:intron
TGCCTGCATAATGTGCCTCG+CGG 0.710446 3_1:+55587277 None:intergenic
AGAGATTAGATAAAACCGCG+AGG 0.761190 3_1:-55587292 Msa0363320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATGTAATAATTTTAGA+TGG + chr3_1:55587889-55587908 None:intergenic 10.0%
!!! AAAAATGTAATAATTTTAGA+TGG + chr3_1:55587889-55587908 None:intergenic 10.0%
!! TAATCATTTGTAAAAATTGT+TGG - chr3_1:55586736-55586755 Msa0363320:intron 15.0%
!!! TTCTTTTTATTCTCTTTTTT+GGG + chr3_1:55587320-55587339 None:intergenic 15.0%
!!! ATTCTTTTTATTCTCTTTTT+TGG + chr3_1:55587321-55587340 None:intergenic 15.0%
!! TTATTTGAATTATTGTTATG+AGG - chr3_1:55587377-55587396 Msa0363320:CDS 15.0%
!!! TTAATAATTTTGTTATACTC+AGG - chr3_1:55587789-55587808 Msa0363320:intron 15.0%
!!! CTAGATTCTTTATTTTAATA+TGG - chr3_1:55587856-55587875 Msa0363320:intron 15.0%
!! TAATCATTTGTAAAAATTGT+TGG - chr3_1:55586736-55586755 Msa0363320:intron 15.0%
!!! TTCTTTTTATTCTCTTTTTT+GGG + chr3_1:55587320-55587339 None:intergenic 15.0%
!!! ATTCTTTTTATTCTCTTTTT+TGG + chr3_1:55587321-55587340 None:intergenic 15.0%
!! TTATTTGAATTATTGTTATG+AGG - chr3_1:55587377-55587396 Msa0363320:CDS 15.0%
!!! TTAATAATTTTGTTATACTC+AGG - chr3_1:55587789-55587808 Msa0363320:intron 15.0%
!!! CTAGATTCTTTATTTTAATA+TGG - chr3_1:55587856-55587875 Msa0363320:intron 15.0%
!!! TTTTTATGTCTTGTTTTTGT+TGG - chr3_1:55586326-55586345 Msa0363320:intron 20.0%
!! AGATATACATAAATTATGGA+AGG + chr3_1:55587118-55587137 None:intergenic 20.0%
!! AAGCAGATATACATAAATTA+TGG + chr3_1:55587122-55587141 None:intergenic 20.0%
!!! TCTCTATTTTTTGACTTTTA+AGG - chr3_1:55587249-55587268 Msa0363320:intron 20.0%
!!! TATTTTTTGACTTTTAAGGA+GGG - chr3_1:55587253-55587272 Msa0363320:intron 20.0%
!!! AGAATATGATGATGAATTTA+AGG - chr3_1:55587351-55587370 Msa0363320:CDS 20.0%
!!! GTATTTTCTAAAGTAACATA+AGG + chr3_1:55587478-55587497 None:intergenic 20.0%
!! AAGCACAATTAACTTTAAAT+TGG + chr3_1:55587580-55587599 None:intergenic 20.0%
!!! TAGTGTCAGTTTTTATTTTA+GGG + chr3_1:55587742-55587761 None:intergenic 20.0%
!!! TTTTTATGTCTTGTTTTTGT+TGG - chr3_1:55586326-55586345 Msa0363320:intron 20.0%
!! AGATATACATAAATTATGGA+AGG + chr3_1:55587118-55587137 None:intergenic 20.0%
!! AAGCAGATATACATAAATTA+TGG + chr3_1:55587122-55587141 None:intergenic 20.0%
!!! TCTCTATTTTTTGACTTTTA+AGG - chr3_1:55587249-55587268 Msa0363320:intron 20.0%
!!! TATTTTTTGACTTTTAAGGA+GGG - chr3_1:55587253-55587272 Msa0363320:intron 20.0%
!!! AGAATATGATGATGAATTTA+AGG - chr3_1:55587351-55587370 Msa0363320:CDS 20.0%
!!! GTATTTTCTAAAGTAACATA+AGG + chr3_1:55587478-55587497 None:intergenic 20.0%
!! AAGCACAATTAACTTTAAAT+TGG + chr3_1:55587580-55587599 None:intergenic 20.0%
!!! TAGTGTCAGTTTTTATTTTA+GGG + chr3_1:55587742-55587761 None:intergenic 20.0%
! TAATAAAACAGCCTAATAAC+CGG + chr3_1:55586182-55586201 None:intergenic 25.0%
!!! GTTTTATTAGTTGAGCAATT+AGG - chr3_1:55586193-55586212 Msa0363320:three_prime_UTR 25.0%
! AAAAAGTTGTTTATCCAAGT+TGG - chr3_1:55586382-55586401 Msa0363320:intron 25.0%
! AAAAGTTGTTTATCCAAGTT+GGG - chr3_1:55586383-55586402 Msa0363320:intron 25.0%
! TAATCTCTTAAATTTGATGC+GGG + chr3_1:55586640-55586659 None:intergenic 25.0%
! CTAATCTCTTAAATTTGATG+CGG + chr3_1:55586641-55586660 None:intergenic 25.0%
!! CTTCTTTGTCTAAAATCTAA+AGG + chr3_1:55586775-55586794 None:intergenic 25.0%
! ACTGTTATGTCATATATCAT+AGG - chr3_1:55586949-55586968 Msa0363320:CDS 25.0%
!!! ATATTCATGCTATTTTGAAC+TGG - chr3_1:55587202-55587221 Msa0363320:intron 25.0%
! AAAATAGAGACATAAATCAC+AGG + chr3_1:55587239-55587258 None:intergenic 25.0%
!!! CTATTTTTTGACTTTTAAGG+AGG - chr3_1:55587252-55587271 Msa0363320:intron 25.0%
!! GGTCTGTTTGAATAAATTTT+TGG + chr3_1:55587457-55587476 None:intergenic 25.0%
!!! CTAGTGTCAGTTTTTATTTT+AGG + chr3_1:55587743-55587762 None:intergenic 25.0%
! TTAATCACAAAAATTCCAAC+AGG + chr3_1:55587773-55587792 None:intergenic 25.0%
! CTTCAAAAAAATTCAGAGTT+GGG + chr3_1:55587998-55588017 None:intergenic 25.0%
! TCTTCAAAAAAATTCAGAGT+TGG + chr3_1:55587999-55588018 None:intergenic 25.0%
! TAATAAAACAGCCTAATAAC+CGG + chr3_1:55586182-55586201 None:intergenic 25.0%
!!! GTTTTATTAGTTGAGCAATT+AGG - chr3_1:55586193-55586212 Msa0363320:three_prime_UTR 25.0%
! AAAAAGTTGTTTATCCAAGT+TGG - chr3_1:55586382-55586401 Msa0363320:intron 25.0%
! AAAAGTTGTTTATCCAAGTT+GGG - chr3_1:55586383-55586402 Msa0363320:intron 25.0%
! TAATCTCTTAAATTTGATGC+GGG + chr3_1:55586640-55586659 None:intergenic 25.0%
! CTAATCTCTTAAATTTGATG+CGG + chr3_1:55586641-55586660 None:intergenic 25.0%
!! CTTCTTTGTCTAAAATCTAA+AGG + chr3_1:55586775-55586794 None:intergenic 25.0%
! ACTGTTATGTCATATATCAT+AGG - chr3_1:55586949-55586968 Msa0363320:CDS 25.0%
!!! ATATTCATGCTATTTTGAAC+TGG - chr3_1:55587202-55587221 Msa0363320:intron 25.0%
! AAAATAGAGACATAAATCAC+AGG + chr3_1:55587239-55587258 None:intergenic 25.0%
!!! CTATTTTTTGACTTTTAAGG+AGG - chr3_1:55587252-55587271 Msa0363320:intron 25.0%
!! GGTCTGTTTGAATAAATTTT+TGG + chr3_1:55587457-55587476 None:intergenic 25.0%
!!! CTAGTGTCAGTTTTTATTTT+AGG + chr3_1:55587743-55587762 None:intergenic 25.0%
! TTAATCACAAAAATTCCAAC+AGG + chr3_1:55587773-55587792 None:intergenic 25.0%
! CTTCAAAAAAATTCAGAGTT+GGG + chr3_1:55587998-55588017 None:intergenic 25.0%
! TCTTCAAAAAAATTCAGAGT+TGG + chr3_1:55587999-55588018 None:intergenic 25.0%
AATAAAACAGCCTAATAACC+GGG + chr3_1:55586181-55586200 None:intergenic 30.0%
TTTGTCACAATTTCTTGTGT+AGG - chr3_1:55586431-55586450 Msa0363320:intron 30.0%
AGAATCAACAGAATTTGTCT+CGG + chr3_1:55586580-55586599 None:intergenic 30.0%
AATCTCTTAAATTTGATGCG+GGG + chr3_1:55586639-55586658 None:intergenic 30.0%
! ATATAACCAGGCTTTATATC+AGG + chr3_1:55586830-55586849 None:intergenic 30.0%
! TCATATATCATAGGAAGTAC+CGG - chr3_1:55586958-55586977 Msa0363320:CDS 30.0%
! AGGCGTGTTTCTATTATAAA+AGG - chr3_1:55586989-55587008 Msa0363320:intron 30.0%
! GGCGTGTTTCTATTATAAAA+GGG - chr3_1:55586990-55587009 Msa0363320:intron 30.0%
! TAAAAGGGATTGTTATTGCA+AGG - chr3_1:55587005-55587024 Msa0363320:intron 30.0%
!! ATAAGAGTATCAATTAGCTC+AGG + chr3_1:55587625-55587644 None:intergenic 30.0%
CTAATTGATACTCTTATGAC+AGG - chr3_1:55587628-55587647 Msa0363320:intron 30.0%
ACCTAACATAAAGGAGATAA+AGG - chr3_1:55587656-55587675 Msa0363320:intron 30.0%
ACCTTTATCTCCTTTATGTT+AGG + chr3_1:55587660-55587679 None:intergenic 30.0%
AGAATGCATGTGAATACATA+TGG + chr3_1:55587819-55587838 None:intergenic 30.0%
AAAACACGATATGATCGAAA+GGG + chr3_1:55587918-55587937 None:intergenic 30.0%
AATAAAACAGCCTAATAACC+GGG + chr3_1:55586181-55586200 None:intergenic 30.0%
TTTGTCACAATTTCTTGTGT+AGG - chr3_1:55586431-55586450 Msa0363320:intron 30.0%
AGAATCAACAGAATTTGTCT+CGG + chr3_1:55586580-55586599 None:intergenic 30.0%
AATCTCTTAAATTTGATGCG+GGG + chr3_1:55586639-55586658 None:intergenic 30.0%
! ATATAACCAGGCTTTATATC+AGG + chr3_1:55586830-55586849 None:intergenic 30.0%
! TCATATATCATAGGAAGTAC+CGG - chr3_1:55586958-55586977 Msa0363320:CDS 30.0%
! AGGCGTGTTTCTATTATAAA+AGG - chr3_1:55586989-55587008 Msa0363320:intron 30.0%
! GGCGTGTTTCTATTATAAAA+GGG - chr3_1:55586990-55587009 Msa0363320:intron 30.0%
! TAAAAGGGATTGTTATTGCA+AGG - chr3_1:55587005-55587024 Msa0363320:intron 30.0%
!! ATAAGAGTATCAATTAGCTC+AGG + chr3_1:55587625-55587644 None:intergenic 30.0%
CTAATTGATACTCTTATGAC+AGG - chr3_1:55587628-55587647 Msa0363320:intron 30.0%
ACCTAACATAAAGGAGATAA+AGG - chr3_1:55587656-55587675 Msa0363320:intron 30.0%
ACCTTTATCTCCTTTATGTT+AGG + chr3_1:55587660-55587679 None:intergenic 30.0%
AGAATGCATGTGAATACATA+TGG + chr3_1:55587819-55587838 None:intergenic 30.0%
AAAACACGATATGATCGAAA+GGG + chr3_1:55587918-55587937 None:intergenic 30.0%
!!! ATCTGATTTTTGCTGTTGTC+AGG - chr3_1:55585984-55586003 Msa0363320:three_prime_UTR 35.0%
!! GATATTGGGTTGATTATTGG+TGG - chr3_1:55586078-55586097 Msa0363320:three_prime_UTR 35.0%
! CAAGTTGGGATAATCTCTTA+GGG - chr3_1:55586397-55586416 Msa0363320:intron 35.0%
! GATTTTAGACAAAGAAGCAG+TGG - chr3_1:55586778-55586797 Msa0363320:intron 35.0%
TTATATTGTGCAGCTCAAAG+TGG - chr3_1:55586844-55586863 Msa0363320:intron 35.0%
ACACTACATTCAGACTTAGA+AGG - chr3_1:55587044-55587063 Msa0363320:intron 35.0%
TACATTCAGACTTAGAAGGA+TGG - chr3_1:55587048-55587067 Msa0363320:intron 35.0%
TGGAAGGTAATACTTACAGT+GGG + chr3_1:55587102-55587121 None:intergenic 35.0%
ATGGAAGGTAATACTTACAG+TGG + chr3_1:55587103-55587122 None:intergenic 35.0%
! AAGGAGGGTGTAGTTAAAAT+AGG - chr3_1:55587268-55587287 Msa0363320:CDS 35.0%
CAGGTATTCACCTAACATAA+AGG - chr3_1:55587647-55587666 Msa0363320:intron 35.0%
CAAAACACGATATGATCGAA+AGG + chr3_1:55587919-55587938 None:intergenic 35.0%
! CTTTCGATCATATCGTGTTT+TGG - chr3_1:55587917-55587936 Msa0363320:CDS 35.0%
!!! GATCATATCGTGTTTTGGAA+AGG - chr3_1:55587922-55587941 Msa0363320:CDS 35.0%
!!! ATCTGATTTTTGCTGTTGTC+AGG - chr3_1:55585984-55586003 Msa0363320:three_prime_UTR 35.0%
!! GATATTGGGTTGATTATTGG+TGG - chr3_1:55586078-55586097 Msa0363320:three_prime_UTR 35.0%
! CAAGTTGGGATAATCTCTTA+GGG - chr3_1:55586397-55586416 Msa0363320:intron 35.0%
! GATTTTAGACAAAGAAGCAG+TGG - chr3_1:55586778-55586797 Msa0363320:intron 35.0%
TTATATTGTGCAGCTCAAAG+TGG - chr3_1:55586844-55586863 Msa0363320:intron 35.0%
ACACTACATTCAGACTTAGA+AGG - chr3_1:55587044-55587063 Msa0363320:intron 35.0%
TACATTCAGACTTAGAAGGA+TGG - chr3_1:55587048-55587067 Msa0363320:intron 35.0%
TGGAAGGTAATACTTACAGT+GGG + chr3_1:55587102-55587121 None:intergenic 35.0%
ATGGAAGGTAATACTTACAG+TGG + chr3_1:55587103-55587122 None:intergenic 35.0%
! AAGGAGGGTGTAGTTAAAAT+AGG - chr3_1:55587268-55587287 Msa0363320:CDS 35.0%
CAGGTATTCACCTAACATAA+AGG - chr3_1:55587647-55587666 Msa0363320:intron 35.0%
CAAAACACGATATGATCGAA+AGG + chr3_1:55587919-55587938 None:intergenic 35.0%
! CTTTCGATCATATCGTGTTT+TGG - chr3_1:55587917-55587936 Msa0363320:CDS 35.0%
!!! GATCATATCGTGTTTTGGAA+AGG - chr3_1:55587922-55587941 Msa0363320:CDS 35.0%
!! GCGGATATTGGGTTGATTAT+TGG - chr3_1:55586075-55586094 Msa0363320:three_prime_UTR 40.0%
ATTGGTGGAATTAGTGTCAG+TGG - chr3_1:55586093-55586112 Msa0363320:three_prime_UTR 40.0%
TTGGTGGAATTAGTGTCAGT+GGG - chr3_1:55586094-55586113 Msa0363320:three_prime_UTR 40.0%
TATCTGCATACTGTCTTAGC+AGG - chr3_1:55586243-55586262 Msa0363320:CDS 40.0%
CCTAAGAGATTATCCCAACT+TGG + chr3_1:55586399-55586418 None:intergenic 40.0%
! CCAAGTTGGGATAATCTCTT+AGG - chr3_1:55586396-55586415 Msa0363320:intron 40.0%
ATTACCAGACTCATCTCTAG+AGG + chr3_1:55586484-55586503 None:intergenic 40.0%
TCTAGAGATGAGTCTGGTAA+TGG - chr3_1:55586483-55586502 Msa0363320:intron 40.0%
ATGCTGAAATGAAGGCAATC+TGG + chr3_1:55586514-55586533 None:intergenic 40.0%
AGATTGCCTTCATTTCAGCA+TGG - chr3_1:55586513-55586532 Msa0363320:intron 40.0%
ACAGAACCATGCTGAAATGA+AGG + chr3_1:55586522-55586541 None:intergenic 40.0%
TAAACCTGCTTCGGAAATGA+GGG + chr3_1:55586551-55586570 None:intergenic 40.0%
ATAAACCTGCTTCGGAAATG+AGG + chr3_1:55586552-55586571 None:intergenic 40.0%
AGAGATTAGATAAAACCGCG+AGG - chr3_1:55586652-55586671 Msa0363320:intron 40.0%
TTTAGACAAAGAAGCAGTGG+AGG - chr3_1:55586781-55586800 Msa0363320:intron 40.0%
TTGAGCTGCACAATATAACC+AGG + chr3_1:55586842-55586861 None:intergenic 40.0%
GTTATTGCAAGGCGTAATTG+TGG - chr3_1:55587016-55587035 Msa0363320:intron 40.0%
AACTCGACTCAACAAAACTC+CGG + chr3_1:55587162-55587181 None:intergenic 40.0%
! CAATGTGCGTTTTGTGTACA+TGG - chr3_1:55587521-55587540 Msa0363320:intron 40.0%
CAACACAAAACGCATTGAAC+TGG + chr3_1:55587550-55587569 None:intergenic 40.0%
!! GCGGATATTGGGTTGATTAT+TGG - chr3_1:55586075-55586094 Msa0363320:three_prime_UTR 40.0%
ATTGGTGGAATTAGTGTCAG+TGG - chr3_1:55586093-55586112 Msa0363320:three_prime_UTR 40.0%
TTGGTGGAATTAGTGTCAGT+GGG - chr3_1:55586094-55586113 Msa0363320:three_prime_UTR 40.0%
TATCTGCATACTGTCTTAGC+AGG - chr3_1:55586243-55586262 Msa0363320:CDS 40.0%
CCTAAGAGATTATCCCAACT+TGG + chr3_1:55586399-55586418 None:intergenic 40.0%
! CCAAGTTGGGATAATCTCTT+AGG - chr3_1:55586396-55586415 Msa0363320:intron 40.0%
ATTACCAGACTCATCTCTAG+AGG + chr3_1:55586484-55586503 None:intergenic 40.0%
TCTAGAGATGAGTCTGGTAA+TGG - chr3_1:55586483-55586502 Msa0363320:intron 40.0%
ATGCTGAAATGAAGGCAATC+TGG + chr3_1:55586514-55586533 None:intergenic 40.0%
AGATTGCCTTCATTTCAGCA+TGG - chr3_1:55586513-55586532 Msa0363320:intron 40.0%
ACAGAACCATGCTGAAATGA+AGG + chr3_1:55586522-55586541 None:intergenic 40.0%
TAAACCTGCTTCGGAAATGA+GGG + chr3_1:55586551-55586570 None:intergenic 40.0%
ATAAACCTGCTTCGGAAATG+AGG + chr3_1:55586552-55586571 None:intergenic 40.0%
AGAGATTAGATAAAACCGCG+AGG - chr3_1:55586652-55586671 Msa0363320:intron 40.0%
TTTAGACAAAGAAGCAGTGG+AGG - chr3_1:55586781-55586800 Msa0363320:intron 40.0%
TTGAGCTGCACAATATAACC+AGG + chr3_1:55586842-55586861 None:intergenic 40.0%
GTTATTGCAAGGCGTAATTG+TGG - chr3_1:55587016-55587035 Msa0363320:intron 40.0%
AACTCGACTCAACAAAACTC+CGG + chr3_1:55587162-55587181 None:intergenic 40.0%
! CAATGTGCGTTTTGTGTACA+TGG - chr3_1:55587521-55587540 Msa0363320:intron 40.0%
CAACACAAAACGCATTGAAC+TGG + chr3_1:55587550-55587569 None:intergenic 40.0%
TGGTGGAATTAGTGTCAGTG+GGG - chr3_1:55586095-55586114 Msa0363320:three_prime_UTR 45.0%
CTTCATGATCCAGTGAAACC+CGG - chr3_1:55586160-55586179 Msa0363320:three_prime_UTR 45.0%
TCCAGTGAAACCCGGTTATT+AGG - chr3_1:55586168-55586187 Msa0363320:three_prime_UTR 45.0%
CTAGAGGATACATTCGTCTG+AGG + chr3_1:55586468-55586487 None:intergenic 45.0%
! GTATCCTCTAGAGATGAGTC+TGG - chr3_1:55586477-55586496 Msa0363320:intron 45.0%
CGGTAGACATAAACCTGCTT+CGG + chr3_1:55586560-55586579 None:intergenic 45.0%
! AGGCAATTCAGGTTCTGGTA+AGG + chr3_1:55586616-55586635 None:intergenic 45.0%
CTCTTAAATTTGATGCGGGG+AGG + chr3_1:55586636-55586655 None:intergenic 45.0%
! GAGGTACCTGATATAAAGCC+TGG - chr3_1:55586821-55586840 Msa0363320:intron 45.0%
AGACTTAGAAGGATGGTAGC+TGG - chr3_1:55587055-55587074 Msa0363320:intron 45.0%
GACTTAGAAGGATGGTAGCT+GGG - chr3_1:55587056-55587075 Msa0363320:intron 45.0%
ACTTAGAAGGATGGTAGCTG+GGG - chr3_1:55587057-55587076 Msa0363320:intron 45.0%
AGCCAAACTCTACTACCTGA+GGG - chr3_1:55587704-55587723 Msa0363320:intron 45.0%
ATCCCTCAGGTAGTAGAGTT+TGG + chr3_1:55587709-55587728 None:intergenic 45.0%
! GGGCATTCATTTTATCCCTC+AGG + chr3_1:55587722-55587741 None:intergenic 45.0%
TGGTGGAATTAGTGTCAGTG+GGG - chr3_1:55586095-55586114 Msa0363320:three_prime_UTR 45.0%
CTTCATGATCCAGTGAAACC+CGG - chr3_1:55586160-55586179 Msa0363320:three_prime_UTR 45.0%
TCCAGTGAAACCCGGTTATT+AGG - chr3_1:55586168-55586187 Msa0363320:three_prime_UTR 45.0%
CTAGAGGATACATTCGTCTG+AGG + chr3_1:55586468-55586487 None:intergenic 45.0%
! GTATCCTCTAGAGATGAGTC+TGG - chr3_1:55586477-55586496 Msa0363320:intron 45.0%
CGGTAGACATAAACCTGCTT+CGG + chr3_1:55586560-55586579 None:intergenic 45.0%
! AGGCAATTCAGGTTCTGGTA+AGG + chr3_1:55586616-55586635 None:intergenic 45.0%
CTCTTAAATTTGATGCGGGG+AGG + chr3_1:55586636-55586655 None:intergenic 45.0%
! GAGGTACCTGATATAAAGCC+TGG - chr3_1:55586821-55586840 Msa0363320:intron 45.0%
AGACTTAGAAGGATGGTAGC+TGG - chr3_1:55587055-55587074 Msa0363320:intron 45.0%
GACTTAGAAGGATGGTAGCT+GGG - chr3_1:55587056-55587075 Msa0363320:intron 45.0%
ACTTAGAAGGATGGTAGCTG+GGG - chr3_1:55587057-55587076 Msa0363320:intron 45.0%
AGCCAAACTCTACTACCTGA+GGG - chr3_1:55587704-55587723 Msa0363320:intron 45.0%
ATCCCTCAGGTAGTAGAGTT+TGG + chr3_1:55587709-55587728 None:intergenic 45.0%
! GGGCATTCATTTTATCCCTC+AGG + chr3_1:55587722-55587741 None:intergenic 45.0%
!! GTCCACAGTTCTGGTTTGTG+TGG - chr3_1:55586008-55586027 Msa0363320:three_prime_UTR 50.0%
GTCCACACAAACCAGAACTG+TGG + chr3_1:55586013-55586032 None:intergenic 50.0%
!! CAGTTCTGGTTTGTGTGGAC+AGG - chr3_1:55586013-55586032 Msa0363320:three_prime_UTR 50.0%
TTCAGGTGCTAAGCAGAGCT+GGG - chr3_1:55586043-55586062 Msa0363320:three_prime_UTR 50.0%
!! GGTCTGGTGTTGCGGATATT+GGG - chr3_1:55586064-55586083 Msa0363320:three_prime_UTR 50.0%
GCCTAATAACCGGGTTTCAC+TGG + chr3_1:55586172-55586191 None:intergenic 50.0%
! CTTAGCAGGCTGTGTCCTTT+TGG - chr3_1:55586257-55586276 Msa0363320:CDS 50.0%
! AGGCTGTGTCCTTTTGGTCT+GGG - chr3_1:55586263-55586282 Msa0363320:CDS 50.0%
! GGGGGTTTGGCATTTTTCGT+TGG - chr3_1:55586283-55586302 Msa0363320:CDS 50.0%
TTGTCCCTCATTTCCGAAGC+AGG - chr3_1:55586544-55586563 Msa0363320:intron 50.0%
AGACAAAGAAGCAGTGGAGG+AGG - chr3_1:55586784-55586803 Msa0363320:intron 50.0%
GGAGGTGAAAGAACAGAGAG+AGG - chr3_1:55586802-55586821 Msa0363320:intron 50.0%
!! AGGAAGTACCGGAGAACAAG+AGG - chr3_1:55586969-55586988 Msa0363320:CDS 50.0%
GAAACACGCCTCTTGTTCTC+CGG + chr3_1:55586980-55586999 None:intergenic 50.0%
CTGCTTGTAACTCTGCACCT+TGG - chr3_1:55587136-55587155 Msa0363320:CDS 50.0%
TTGTAACTCTGCACCTTGGC+CGG - chr3_1:55587140-55587159 Msa0363320:CDS 50.0%
ACTCAACAAAACTCCGGCCA+AGG + chr3_1:55587156-55587175 None:intergenic 50.0%
GAGCCAAACTCTACTACCTG+AGG - chr3_1:55587703-55587722 Msa0363320:intron 50.0%
!! GACACTAGTCTTCAGCCTGT+TGG - chr3_1:55587755-55587774 Msa0363320:intron 50.0%
!! GTCCACAGTTCTGGTTTGTG+TGG - chr3_1:55586008-55586027 Msa0363320:three_prime_UTR 50.0%
GTCCACACAAACCAGAACTG+TGG + chr3_1:55586013-55586032 None:intergenic 50.0%
!! CAGTTCTGGTTTGTGTGGAC+AGG - chr3_1:55586013-55586032 Msa0363320:three_prime_UTR 50.0%
TTCAGGTGCTAAGCAGAGCT+GGG - chr3_1:55586043-55586062 Msa0363320:three_prime_UTR 50.0%
!! GGTCTGGTGTTGCGGATATT+GGG - chr3_1:55586064-55586083 Msa0363320:three_prime_UTR 50.0%
GCCTAATAACCGGGTTTCAC+TGG + chr3_1:55586172-55586191 None:intergenic 50.0%
! CTTAGCAGGCTGTGTCCTTT+TGG - chr3_1:55586257-55586276 Msa0363320:CDS 50.0%
! AGGCTGTGTCCTTTTGGTCT+GGG - chr3_1:55586263-55586282 Msa0363320:CDS 50.0%
! GGGGGTTTGGCATTTTTCGT+TGG - chr3_1:55586283-55586302 Msa0363320:CDS 50.0%
TTGTCCCTCATTTCCGAAGC+AGG - chr3_1:55586544-55586563 Msa0363320:intron 50.0%
AGACAAAGAAGCAGTGGAGG+AGG - chr3_1:55586784-55586803 Msa0363320:intron 50.0%
GGAGGTGAAAGAACAGAGAG+AGG - chr3_1:55586802-55586821 Msa0363320:intron 50.0%
!! AGGAAGTACCGGAGAACAAG+AGG - chr3_1:55586969-55586988 Msa0363320:CDS 50.0%
GAAACACGCCTCTTGTTCTC+CGG + chr3_1:55586980-55586999 None:intergenic 50.0%
CTGCTTGTAACTCTGCACCT+TGG - chr3_1:55587136-55587155 Msa0363320:CDS 50.0%
TTGTAACTCTGCACCTTGGC+CGG - chr3_1:55587140-55587159 Msa0363320:CDS 50.0%
ACTCAACAAAACTCCGGCCA+AGG + chr3_1:55587156-55587175 None:intergenic 50.0%
GAGCCAAACTCTACTACCTG+AGG - chr3_1:55587703-55587722 Msa0363320:intron 50.0%
!! GACACTAGTCTTCAGCCTGT+TGG - chr3_1:55587755-55587774 Msa0363320:intron 50.0%
TTGTCAGGCGTCCACAGTTC+TGG - chr3_1:55585999-55586018 Msa0363320:three_prime_UTR 55.0%
! CTGGTTTGTGTGGACAGGTC+TGG - chr3_1:55586018-55586037 Msa0363320:three_prime_UTR 55.0%
TGTGGACAGGTCTGGACTTC+AGG - chr3_1:55586026-55586045 Msa0363320:three_prime_UTR 55.0%
CTTCAGGTGCTAAGCAGAGC+TGG - chr3_1:55586042-55586061 Msa0363320:three_prime_UTR 55.0%
!! GGGTCTGGTGTTGCGGATAT+TGG - chr3_1:55586063-55586082 Msa0363320:three_prime_UTR 55.0%
GGAATTAGTGTCAGTGGGGC+AGG - chr3_1:55586099-55586118 Msa0363320:three_prime_UTR 55.0%
! CAGGCTGTGTCCTTTTGGTC+TGG - chr3_1:55586262-55586281 Msa0363320:CDS 55.0%
! GGCTGTGTCCTTTTGGTCTG+GGG - chr3_1:55586264-55586283 Msa0363320:CDS 55.0%
! GCTGTGTCCTTTTGGTCTGG+GGG - chr3_1:55586265-55586284 Msa0363320:CDS 55.0%
! GTCCTTTTGGTCTGGGGGTT+TGG - chr3_1:55586270-55586289 Msa0363320:CDS 55.0%
TGCCAAACCCCCAGACCAAA+AGG + chr3_1:55586275-55586294 None:intergenic 55.0%
TTGATGCGGGGAGGCAATTC+AGG + chr3_1:55586627-55586646 None:intergenic 55.0%
AACCGCGAGGCACATTATGC+AGG - chr3_1:55586665-55586684 Msa0363320:intron 55.0%
TGCCTGCATAATGTGCCTCG+CGG + chr3_1:55586670-55586689 None:intergenic 55.0%
AGAAGGATGGTAGCTGGGGT+TGG - chr3_1:55587061-55587080 Msa0363320:intron 55.0%
GAAGGATGGTAGCTGGGGTT+GGG - chr3_1:55587062-55587081 Msa0363320:intron 55.0%
TTGTCAGGCGTCCACAGTTC+TGG - chr3_1:55585999-55586018 Msa0363320:three_prime_UTR 55.0%
! CTGGTTTGTGTGGACAGGTC+TGG - chr3_1:55586018-55586037 Msa0363320:three_prime_UTR 55.0%
TGTGGACAGGTCTGGACTTC+AGG - chr3_1:55586026-55586045 Msa0363320:three_prime_UTR 55.0%
CTTCAGGTGCTAAGCAGAGC+TGG - chr3_1:55586042-55586061 Msa0363320:three_prime_UTR 55.0%
!! GGGTCTGGTGTTGCGGATAT+TGG - chr3_1:55586063-55586082 Msa0363320:three_prime_UTR 55.0%
GGAATTAGTGTCAGTGGGGC+AGG - chr3_1:55586099-55586118 Msa0363320:three_prime_UTR 55.0%
! CAGGCTGTGTCCTTTTGGTC+TGG - chr3_1:55586262-55586281 Msa0363320:CDS 55.0%
! GGCTGTGTCCTTTTGGTCTG+GGG - chr3_1:55586264-55586283 Msa0363320:CDS 55.0%
! GCTGTGTCCTTTTGGTCTGG+GGG - chr3_1:55586265-55586284 Msa0363320:CDS 55.0%
! GTCCTTTTGGTCTGGGGGTT+TGG - chr3_1:55586270-55586289 Msa0363320:CDS 55.0%
TGCCAAACCCCCAGACCAAA+AGG + chr3_1:55586275-55586294 None:intergenic 55.0%
TTGATGCGGGGAGGCAATTC+AGG + chr3_1:55586627-55586646 None:intergenic 55.0%
AACCGCGAGGCACATTATGC+AGG - chr3_1:55586665-55586684 Msa0363320:intron 55.0%
TGCCTGCATAATGTGCCTCG+CGG + chr3_1:55586670-55586689 None:intergenic 55.0%
AGAAGGATGGTAGCTGGGGT+TGG - chr3_1:55587061-55587080 Msa0363320:intron 55.0%
GAAGGATGGTAGCTGGGGTT+GGG - chr3_1:55587062-55587081 Msa0363320:intron 55.0%
GTGCTAAGCAGAGCTGGGTC+TGG - chr3_1:55586048-55586067 Msa0363320:three_prime_UTR 60.0%
! CAGAGCTGGGTCTGGTGTTG+CGG - chr3_1:55586056-55586075 Msa0363320:three_prime_UTR 60.0%
! CGGGGAGGCAATTCAGGTTC+TGG + chr3_1:55586621-55586640 None:intergenic 60.0%
GTGCTAAGCAGAGCTGGGTC+TGG - chr3_1:55586048-55586067 Msa0363320:three_prime_UTR 60.0%
! CAGAGCTGGGTCTGGTGTTG+CGG - chr3_1:55586056-55586075 Msa0363320:three_prime_UTR 60.0%
! CGGGGAGGCAATTCAGGTTC+TGG + chr3_1:55586621-55586640 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 55585933 55588033 55585933 ID=Msa0363320;Name=Msa0363320
chr3_1 mRNA 55585933 55588033 55585933 ID=Msa0363320-mRNA-1;Parent=Msa0363320;Name=Msa0363320-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.4|0.33|0.66|0.8|0.83|6|284|225
chr3_1 exon 55585933 55586316 55585933 ID=Msa0363320-mRNA-1:exon:10191;Parent=Msa0363320-mRNA-1
chr3_1 exon 55586862 55586995 55586862 ID=Msa0363320-mRNA-1:exon:10190;Parent=Msa0363320-mRNA-1
chr3_1 exon 55587101 55587187 55587101 ID=Msa0363320-mRNA-1:exon:10189;Parent=Msa0363320-mRNA-1
chr3_1 exon 55587265 55587513 55587265 ID=Msa0363320-mRNA-1:exon:10188;Parent=Msa0363320-mRNA-1
chr3_1 exon 55587859 55587960 55587859 ID=Msa0363320-mRNA-1:exon:10187;Parent=Msa0363320-mRNA-1
chr3_1 exon 55588028 55588033 55588028 ID=Msa0363320-mRNA-1:exon:10186;Parent=Msa0363320-mRNA-1
chr3_1 CDS 55588028 55588033 55588028 ID=Msa0363320-mRNA-1:cds;Parent=Msa0363320-mRNA-1
chr3_1 CDS 55587859 55587960 55587859 ID=Msa0363320-mRNA-1:cds;Parent=Msa0363320-mRNA-1
chr3_1 CDS 55587265 55587513 55587265 ID=Msa0363320-mRNA-1:cds;Parent=Msa0363320-mRNA-1
chr3_1 CDS 55587101 55587187 55587101 ID=Msa0363320-mRNA-1:cds;Parent=Msa0363320-mRNA-1
chr3_1 CDS 55586862 55586995 55586862 ID=Msa0363320-mRNA-1:cds;Parent=Msa0363320-mRNA-1
chr3_1 CDS 55586217 55586316 55586217 ID=Msa0363320-mRNA-1:cds;Parent=Msa0363320-mRNA-1
chr3_1 three_prime_UTR 55585933 55586216 55585933 ID=Msa0363320-mRNA-1:three_prime_utr;Parent=Msa0363320-mRNA-1
Gene Sequence

>Msa0363320

ATGATAGCGTCCACAGTTCTGGTTTGTGTGGACAGGTCTGGACTTCAGGTGCTAAGCAGAGCTGGGTCTGGTGTTGCGGATATTGGGTTGATTATTGGTGGAATTAGTGTTACAGTGTCTCCTCAGACGAATGTATCCTCTAGAGATGAGTCTGGTAATGGTTTGACCAGATTGCCTTCATTTCAGCATGGTTCTGTGCTTGTCCCTCATTTCCGAAGCAGGTTTATGTCTACCGAGACAAATTCTGTTGATTCTTCTGAAGATAGTTCCTTACCAGAACCTGAATTGCCTCCCCGCATCAAATTTAAGAGATTAGATAAAACCGCGAGGCACATTATGCAGGCATGTGACTTCTCTATTTTAGACAAAGAAGCAGTGGAGGAGGTGAAAGAACAGAGAGAGGTACCTGATATAAAGCCTGGTTATATTGTGCAGCTCAAAGTGGAAGTACCGGAGAACAAGAGGCGTGTTTCTATTATAAAAGGGATTGTTATTGCAAGGCGTAATTGTGGACTCAACACTACATTCAGACTTAGAAGGATGGTAGCTGGGGTTGGGATTGAGTCTCTATTCCCACTGTATTCACCTAACATAAAGGAGATAAAGGTGCTTGACAAGAAGAAAGTTCGAAGAGCCAAACTCTACTACCTGAGGGATAAAATGAATGCCCTAAAATAA
Protein sequence

>Msa0363320

MIASTVLVCVDRSGLQVLSRAGSGVADIGLIIGGISVTVSPQTNVSSRDESGNGLTRLPSFQHGSVLVPHFRSRFMSTETNSVDSSEDSSLPEPELPPRIKFKRLDKTARHIMQACDFSILDKEAVEEVKEQREVPDIKPGYIVQLKVEVPENKRRVSIIKGIVIARRNCGLNTTFRLRRMVAGVGIESLFPLYSPNIKEIKVLDKKKVRRAKLYYLRDKMNALK*