Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0368290 | XP_003600750.2 | 75.714 | 140 | 34 | 0 | 1 | 140 | 1 | 140 | 1.39e-67 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0368290 | sp|P62926|ALB1A_PEA | 43.796 | 137 | 63 | 6 | 1 | 135 | 1 | 125 | 7.24e-24 | 92.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0368290 | G7J9G6 | 75.714 | 140 | 34 | 0 | 1 | 140 | 1 | 140 | 6.63e-68 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0045810 | Msa0368290 | 0.807176 | 5.743902e-50 | -8.615850e-47 |
| Msa0046200 | Msa0368290 | 0.806207 | 9.203082e-50 | -8.615850e-47 |
| Msa0062010 | Msa0368290 | 0.816914 | 4.326318e-52 | -8.615850e-47 |
| Msa0063540 | Msa0368290 | 0.811034 | 8.567179e-51 | -8.615850e-47 |
| Msa0190890 | Msa0368290 | 0.800146 | 1.652862e-48 | -8.615850e-47 |
| Msa0220090 | Msa0368290 | 0.819159 | 1.344787e-52 | -8.615850e-47 |
| Msa0233210 | Msa0368290 | 0.801790 | 7.625721e-49 | -8.615850e-47 |
| Msa0368290 | Msa0375860 | 0.800639 | 1.311500e-48 | -8.615850e-47 |
| Msa0368290 | Msa0386910 | 0.802885 | 4.535922e-49 | -8.615850e-47 |
| Msa0368290 | Msa0502970 | 0.803403 | 3.544518e-49 | -8.615850e-47 |
| Msa0368290 | Msa0523720 | 0.823280 | 1.507828e-53 | -8.615850e-47 |
| Msa0368290 | Msa0565700 | 0.823589 | 1.276716e-53 | -8.615850e-47 |
| Msa0368290 | Msa0580100 | 0.916367 | 1.939211e-85 | -8.615850e-47 |
| Msa0368290 | Msa0739720 | 0.822586 | 2.188833e-53 | -8.615850e-47 |
| Msa0368290 | Msa0880910 | 0.811883 | 5.602092e-51 | -8.615850e-47 |
| Msa0368290 | Msa0967780 | 0.810059 | 1.391529e-50 | -8.615850e-47 |
| Msa0368290 | Msa1055450 | 0.814485 | 1.503958e-51 | -8.615850e-47 |
| Msa0368290 | Msa1120680 | 0.817445 | 3.286303e-52 | -8.615850e-47 |
| Msa0368290 | Msa1233630 | 0.806285 | 8.858941e-50 | -8.615850e-47 |
| Msa0368290 | Msa1275850 | 0.808236 | 3.419603e-50 | -8.615850e-47 |
| Msa0368290 | Msa1368420 | 0.805327 | 1.408593e-49 | -8.615850e-47 |
| Msa0368290 | Msa1383220 | 0.803446 | 3.473031e-49 | -8.615850e-47 |
| Msa0368290 | Msa1455360 | 0.802820 | 4.679371e-49 | -8.615850e-47 |
| Msa0261400 | Msa0368290 | 0.818410 | 1.989317e-52 | -8.615850e-47 |
| Msa0299030 | Msa0368290 | 0.827284 | 1.703097e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0368290 | MtrunA17_Chr3g0111111 | 75.714 | 140 | 34 | 0 | 1 | 140 | 1 | 140 | 1.28e-71 | 211 |
| Msa0368290 | MtrunA17_Chr3g0111191 | 71.795 | 117 | 33 | 0 | 1 | 117 | 1 | 117 | 1.48e-55 | 169 |
| Msa0368290 | MtrunA17_Chr3g0111281 | 58.865 | 141 | 56 | 2 | 1 | 140 | 1 | 140 | 1.76e-50 | 157 |
| Msa0368290 | MtrunA17_Chr3g0111261 | 58.571 | 140 | 54 | 2 | 1 | 140 | 1 | 136 | 6.53e-47 | 148 |
| Msa0368290 | MtrunA17_Chr3g0111351 | 52.083 | 144 | 57 | 3 | 1 | 140 | 9 | 144 | 8.24e-44 | 140 |
| Msa0368290 | MtrunA17_Chr3g0111081 | 57.664 | 137 | 50 | 5 | 1 | 136 | 1 | 130 | 1.83e-42 | 137 |
| Msa0368290 | MtrunA17_Chr6g0464071 | 52.817 | 142 | 57 | 4 | 1 | 140 | 1 | 134 | 8.12e-42 | 135 |
| Msa0368290 | MtrunA17_Chr3g0111241 | 51.429 | 140 | 58 | 2 | 1 | 140 | 1 | 130 | 1.93e-40 | 132 |
| Msa0368290 | MtrunA17_Chr3g0111131 | 52.593 | 135 | 56 | 4 | 1 | 133 | 1 | 129 | 1.98e-40 | 131 |
| Msa0368290 | MtrunA17_Chr3g0111071 | 54.745 | 137 | 54 | 4 | 1 | 136 | 1 | 130 | 5.01e-40 | 130 |
| Msa0368290 | MtrunA17_Chr3g0111711 | 68.932 | 103 | 29 | 3 | 1 | 102 | 1 | 101 | 6.81e-39 | 127 |
| Msa0368290 | MtrunA17_Chr3g0104571 | 49.286 | 140 | 63 | 4 | 1 | 138 | 1 | 134 | 8.24e-35 | 117 |
| Msa0368290 | MtrunA17_Chr7g0236791 | 46.154 | 143 | 69 | 3 | 1 | 140 | 1 | 138 | 4.36e-33 | 113 |
| Msa0368290 | MtrunA17_Chr7g0236801 | 46.528 | 144 | 68 | 5 | 1 | 140 | 1 | 139 | 1.11e-32 | 112 |
| Msa0368290 | MtrunA17_Chr3g0111221 | 54.167 | 120 | 48 | 3 | 22 | 140 | 1 | 114 | 7.42e-31 | 107 |
| Msa0368290 | MtrunA17_Chr6g0456951 | 44.681 | 141 | 65 | 7 | 1 | 136 | 1 | 133 | 1.05e-29 | 104 |
| Msa0368290 | MtrunA17_Chr6g0468601 | 41.259 | 143 | 67 | 4 | 1 | 136 | 1 | 133 | 2.07e-29 | 103 |
| Msa0368290 | MtrunA17_Chr3g0111201 | 87.037 | 54 | 7 | 0 | 87 | 140 | 3 | 56 | 5.49e-29 | 100 |
| Msa0368290 | MtrunA17_Chr3g0111311 | 53.535 | 99 | 39 | 3 | 22 | 120 | 1 | 92 | 1.31e-28 | 100 |
| Msa0368290 | MtrunA17_Chr1g0153591 | 42.520 | 127 | 55 | 3 | 1 | 126 | 1 | 110 | 2.56e-27 | 97.8 |
| Msa0368290 | MtrunA17_Chr6g0468621 | 41.135 | 141 | 67 | 4 | 1 | 136 | 1 | 130 | 5.19e-27 | 97.8 |
| Msa0368290 | MtrunA17_Chr3g0090571 | 38.961 | 154 | 74 | 6 | 1 | 138 | 1 | 150 | 6.15e-27 | 98.2 |
| Msa0368290 | MtrunA17_Chr7g0232011 | 43.548 | 124 | 59 | 4 | 1 | 123 | 1 | 114 | 4.11e-24 | 90.9 |
| Msa0368290 | MtrunA17_Chr8g0359071 | 38.843 | 121 | 64 | 2 | 3 | 121 | 4 | 116 | 2.34e-23 | 88.6 |
| Msa0368290 | MtrunA17_Chr4g0012121 | 41.781 | 146 | 68 | 5 | 1 | 136 | 1 | 139 | 2.35e-23 | 89.0 |
| Msa0368290 | MtrunA17_Chr8g0344801 | 42.105 | 95 | 48 | 2 | 43 | 136 | 7 | 95 | 7.34e-20 | 78.6 |
| Msa0368290 | MtrunA17_Chr6g0456991 | 44.318 | 88 | 42 | 2 | 43 | 129 | 27 | 108 | 1.94e-19 | 77.8 |
| Msa0368290 | MtrunA17_Chr8g0344771 | 40.678 | 118 | 59 | 6 | 22 | 136 | 1 | 110 | 1.48e-18 | 75.9 |
| Msa0368290 | MtrunA17_Chr7g0231991 | 38.806 | 134 | 59 | 4 | 22 | 138 | 1 | 128 | 2.13e-18 | 75.9 |
| Msa0368290 | MtrunA17_Chr8g0344791 | 39.130 | 115 | 60 | 5 | 22 | 133 | 1 | 108 | 7.31e-18 | 73.9 |
| Msa0368290 | MtrunA17_Chr7g0225821 | 36.719 | 128 | 70 | 5 | 1 | 124 | 1 | 121 | 2.85e-17 | 72.8 |
| Msa0368290 | MtrunA17_Chr8g0357331 | 36.458 | 96 | 40 | 2 | 64 | 138 | 2 | 97 | 3.01e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAATTCTAATCTCAAATA+TGG | 0.216722 | 3_1:-64772607 | Msa0368290:CDS |
| CCATATTTGAGATTAGAATT+TGG | 0.241765 | 3_1:+64772607 | None:intergenic |
| TGTACGATCGGTTATTGTTA+TGG | 0.354008 | 3_1:-64772754 | Msa0368290:CDS |
| GTCTTCTTGCTTGCTGCATT+CGG | 0.367498 | 3_1:-64773043 | Msa0368290:intron |
| TTTGCTTCTACTTTCTTCAT+TGG | 0.374147 | 3_1:+64772847 | None:intergenic |
| CTTGTAAGCGTCTTGTGCTT+CGG | 0.409202 | 3_1:+64772564 | None:intergenic |
| GAAAATCGCAATGTGGTAAG+TGG | 0.449479 | 3_1:-64772724 | Msa0368290:CDS |
| TTGACATATCAATATTAAGC+AGG | 0.480895 | 3_1:+64772493 | None:intergenic |
| TTTCTTGAATTTATAGCTAG+AGG | 0.515321 | 3_1:+64772537 | None:intergenic |
| AGGAACATCCTAAGTTATGT+TGG | 0.542665 | 3_1:-64772681 | Msa0368290:CDS |
| TCATGAGACCAACATAACTT+AGG | 0.547157 | 3_1:+64772673 | None:intergenic |
| AAAGTAGAAGCAAATTGTGA+TGG | 0.553230 | 3_1:-64772838 | Msa0368290:CDS |
| AAACTCGTTGGTTGTACGAT+CGG | 0.575852 | 3_1:-64772766 | Msa0368290:CDS |
| AGCACAAGACGCTTACAAGA+TGG | 0.577959 | 3_1:-64772560 | Msa0368290:CDS |
| AAAATCGCAATGTGGTAAGT+GGG | 0.583821 | 3_1:-64772723 | Msa0368290:CDS |
| GGTTGTAAATGCAAACTCGT+TGG | 0.593162 | 3_1:-64772778 | Msa0368290:CDS |
| GTCTTGTGCTTCGGAGACAG+AGG | 0.642879 | 3_1:+64772573 | None:intergenic |
| TTTATGCTAGACTGTGATAG+CGG | 0.659883 | 3_1:-64772799 | Msa0368290:CDS |
| TACCTGCGAAAATCGCAATG+TGG | 0.731640 | 3_1:-64772731 | Msa0368290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTATTATTATATTAA+TGG | - | chr3_1:64772658-64772677 | Msa0368290:CDS | 0.0% |
| ! | AATGCATGAATGGTATATAT+TGG | + | chr3_1:64772587-64772606 | None:intergenic | 25.0% |
| ! | CATAAATAGAAATGCATGAA+TGG | + | chr3_1:64772597-64772616 | None:intergenic | 25.0% |
| !! | CCATATTTGAGATTAGAATT+TGG | + | chr3_1:64772976-64772995 | None:intergenic | 25.0% |
| ! | CCAAATTCTAATCTCAAATA+TGG | - | chr3_1:64772973-64772992 | Msa0368290:intron | 25.0% |
| ! | TTTCTTGAATTTATAGCTAG+AGG | + | chr3_1:64773046-64773065 | None:intergenic | 25.0% |
| TTTGCTTCTACTTTCTTCAT+TGG | + | chr3_1:64772736-64772755 | None:intergenic | 30.0% | |
| AAAGTAGAAGCAAATTGTGA+TGG | - | chr3_1:64772742-64772761 | Msa0368290:CDS | 30.0% | |
| TCATGATGAATGCACAAAAA+AGG | - | chr3_1:64772924-64772943 | Msa0368290:intron | 30.0% | |
| CATGATGAATGCACAAAAAA+GGG | - | chr3_1:64772925-64772944 | Msa0368290:intron | 30.0% | |
| TTTATGCTAGACTGTGATAG+CGG | - | chr3_1:64772781-64772800 | Msa0368290:CDS | 35.0% | |
| TGTACGATCGGTTATTGTTA+TGG | - | chr3_1:64772826-64772845 | Msa0368290:CDS | 35.0% | |
| AAAATCGCAATGTGGTAAGT+GGG | - | chr3_1:64772857-64772876 | Msa0368290:CDS | 35.0% | |
| ! | GAATTTTCAGAGGAAGGTTA+AGG | - | chr3_1:64772879-64772898 | Msa0368290:intron | 35.0% |
| AGGAACATCCTAAGTTATGT+TGG | - | chr3_1:64772899-64772918 | Msa0368290:intron | 35.0% | |
| TCATGAGACCAACATAACTT+AGG | + | chr3_1:64772910-64772929 | None:intergenic | 35.0% | |
| AATGCACAAAAAAGGGTAGT+GGG | - | chr3_1:64772932-64772951 | Msa0368290:intron | 35.0% | |
| GGTTGTAAATGCAAACTCGT+TGG | - | chr3_1:64772802-64772821 | Msa0368290:CDS | 40.0% | |
| ! | AAACTCGTTGGTTGTACGAT+CGG | - | chr3_1:64772814-64772833 | Msa0368290:CDS | 40.0% |
| GAAAATCGCAATGTGGTAAG+TGG | - | chr3_1:64772856-64772875 | Msa0368290:CDS | 40.0% | |
| ! | TGGTAAGTGGGAATTTTCAG+AGG | - | chr3_1:64772869-64772888 | Msa0368290:intron | 40.0% |
| ! | AAGTGGGAATTTTCAGAGGA+AGG | - | chr3_1:64772873-64772892 | Msa0368290:intron | 40.0% |
| GAATGCACAAAAAAGGGTAG+TGG | - | chr3_1:64772931-64772950 | Msa0368290:intron | 40.0% | |
| ! | GTCTTCTTGCTTGCTGCATT+CGG | - | chr3_1:64772537-64772556 | Msa0368290:CDS | 45.0% |
| TACCTGCGAAAATCGCAATG+TGG | - | chr3_1:64772849-64772868 | Msa0368290:CDS | 45.0% | |
| ! | TACCACATTGCGATTTTCGC+AGG | + | chr3_1:64772854-64772873 | None:intergenic | 45.0% |
| CTTGTAAGCGTCTTGTGCTT+CGG | + | chr3_1:64773019-64773038 | None:intergenic | 45.0% | |
| AGCACAAGACGCTTACAAGA+TGG | - | chr3_1:64773020-64773039 | Msa0368290:intron | 45.0% | |
| GTCTTGTGCTTCGGAGACAG+AGG | + | chr3_1:64773010-64773029 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 64772507 | 64773095 | 64772507 | ID=Msa0368290;Name=Msa0368290 |
| chr3_1 | mRNA | 64772507 | 64773095 | 64772507 | ID=Msa0368290-mRNA-1;Parent=Msa0368290;Name=Msa0368290-mRNA-1;_AED=0.43;_eAED=0.44;_QI=0|1|0|1|1|1|2|0|140 |
| chr3_1 | exon | 64773044 | 64773095 | 64773044 | ID=Msa0368290-mRNA-1:exon:12913;Parent=Msa0368290-mRNA-1 |
| chr3_1 | exon | 64772507 | 64772877 | 64772507 | ID=Msa0368290-mRNA-1:exon:12912;Parent=Msa0368290-mRNA-1 |
| chr3_1 | CDS | 64773044 | 64773095 | 64773044 | ID=Msa0368290-mRNA-1:cds;Parent=Msa0368290-mRNA-1 |
| chr3_1 | CDS | 64772507 | 64772877 | 64772507 | ID=Msa0368290-mRNA-1:cds;Parent=Msa0368290-mRNA-1 |
| Gene Sequence |
| Protein sequence |
