AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0396000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0396000 MtrunA17_Chr1g0164401 93.750 448 9 1 10 457 1 429 0.0 845
Msa0396000 MtrunA17_Chr8g0353261 78.300 447 77 2 12 457 7 434 0.0 689
Msa0396000 MtrunA17_Chr3g0082281 92.542 295 20 2 164 457 27 320 0.0 520
Msa0396000 MtrunA17_Chr3g0133071 51.535 456 191 9 10 456 3 437 6.18e-141 410
Msa0396000 MtrunA17_Chr3g0082301 98.947 95 1 0 67 161 1 95 3.26e-62 197
Msa0396000 MtrunA17_Chr3g0130931 29.707 478 263 16 3 449 6 441 2.94e-51 179
Msa0396000 MtrunA17_Chr1g0212321 33.062 369 210 9 99 456 144 486 1.07e-49 176
Msa0396000 MtrunA17_Chr7g0275731 34.332 367 211 7 99 456 139 484 1.76e-48 173
Msa0396000 MtrunA17_Chr3g0099891 28.571 434 260 13 38 456 56 454 6.79e-47 168
Msa0396000 MtrunA17_Chr4g0001991 32.552 384 220 14 88 457 160 518 7.15e-47 169
Msa0396000 MtrunA17_Chr2g0291201 31.070 486 252 22 17 456 3 451 4.22e-44 160
Msa0396000 MtrunA17_Chr1g0210201 30.641 421 239 12 55 456 81 467 1.70e-43 159
Msa0396000 MtrunA17_Chr1g0165201 32.425 367 216 8 99 456 126 469 9.43e-43 157
Msa0396000 MtrunA17_Chr3g0095891 32.648 389 206 12 99 456 95 458 5.12e-40 149
Msa0396000 MtrunA17_Chr6g0484901 32.603 365 206 11 102 456 157 491 1.08e-39 149
Msa0396000 MtrunA17_Chr8g0390761 27.869 366 226 10 102 456 117 455 1.51e-38 145
Msa0396000 MtrunA17_Chr3g0099901 29.646 452 259 16 38 456 60 485 8.21e-38 144
Msa0396000 MtrunA17_Chr4g0056211 30.941 404 222 16 102 456 75 470 3.26e-37 142
Msa0396000 MtrunA17_Chr2g0310831 28.539 445 262 17 37 457 29 441 3.93e-36 138
Msa0396000 MtrunA17_Chr5g0442921 31.250 384 219 13 99 456 189 553 4.35e-36 140
Msa0396000 MtrunA17_Chr6g0457401 29.241 448 264 16 32 457 21 437 1.50e-35 136
Msa0396000 MtrunA17_Chr3g0110191 29.067 375 223 15 103 457 88 439 6.54e-35 135
Msa0396000 MtrunA17_Chr5g0415951 28.205 468 283 18 10 457 1 435 9.83e-35 134
Msa0396000 MtrunA17_Chr2g0310821 27.549 461 278 16 15 457 21 443 2.90e-34 133
Msa0396000 MtrunA17_Chr3g0087851 27.556 450 269 18 32 457 21 437 8.41e-34 132
Msa0396000 MtrunA17_Chr5g0399171 29.227 414 237 15 88 456 67 469 1.90e-33 131
Msa0396000 MtrunA17_Chr7g0267171 29.412 391 224 16 102 456 91 465 5.94e-33 130
Msa0396000 MtrunA17_Chr7g0239001 28.052 385 224 14 104 456 62 425 6.05e-32 126
Msa0396000 MtrunA17_Chr5g0435871 25.996 477 291 17 1 457 1 435 1.00e-31 126
Msa0396000 MtrunA17_Chr5g0434411 28.272 382 221 16 103 457 83 438 5.51e-31 124
Msa0396000 MtrunA17_Chr5g0435971 26.244 442 271 16 36 457 26 432 1.30e-30 122
Msa0396000 MtrunA17_Chr2g0310801 27.039 466 283 16 15 457 3 434 3.64e-30 121
Msa0396000 MtrunA17_Chr8g0352661 27.021 470 283 19 13 457 3 437 9.46e-30 120
Msa0396000 MtrunA17_Chr8g0386441 25.163 461 291 16 14 457 7 430 2.25e-29 119
Msa0396000 MtrunA17_Chr3g0112051 27.559 381 207 15 101 456 73 409 5.05e-29 118
Msa0396000 MtrunA17_Chr8g0352671 26.539 471 276 20 14 457 10 437 1.15e-28 117
Msa0396000 MtrunA17_Chr5g0395911 29.897 388 221 17 94 457 54 414 3.73e-27 112
Msa0396000 MtrunA17_Chr2g0307991 28.205 390 218 18 97 457 49 405 4.59e-27 112
Msa0396000 MtrunA17_Chr2g0308001 27.807 374 229 15 102 457 59 409 4.83e-26 110
Msa0396000 MtrunA17_Chr5g0395931 27.404 416 230 16 77 457 58 436 8.88e-26 108
Msa0396000 MtrunA17_Chr2g0311451 24.839 467 291 20 14 457 7 436 2.40e-24 105
Msa0396000 MtrunA17_Chr2g0333991 27.123 365 210 16 102 443 89 420 7.67e-24 103
Msa0396000 MtrunA17_Chr2g0320621 24.228 421 266 18 66 457 42 438 1.25e-23 103
Msa0396000 MtrunA17_Chr4g0027681 25.352 426 255 20 66 457 41 437 1.28e-23 103
Msa0396000 MtrunA17_Chr5g0434441 28.424 387 216 17 103 457 70 427 2.30e-23 102
Msa0396000 MtrunA17_Chr2g0309561 26.013 469 248 21 62 456 65 508 3.28e-23 102
Msa0396000 MtrunA17_Chr5g0419291 24.263 441 276 17 36 456 26 428 3.81e-23 101
Msa0396000 MtrunA17_Chr2g0312941 28.382 377 229 13 99 449 85 446 5.76e-23 101
Msa0396000 MtrunA17_Chr6g0487471 26.338 467 285 20 12 457 7 435 1.04e-22 100
Msa0396000 MtrunA17_Chr5g0406001 27.692 390 206 17 102 456 113 461 5.13e-22 98.6
Msa0396000 MtrunA17_Chr1g0179751 25.056 443 269 19 51 457 55 470 7.83e-22 98.2
Msa0396000 MtrunA17_Chr5g0401601 24.485 388 224 19 102 457 89 439 9.61e-22 98.2
Msa0396000 MtrunA17_Chr2g0307461 27.851 377 215 16 102 447 71 421 1.34e-21 96.7
Msa0396000 MtrunA17_Chr7g0265331 25.445 393 223 19 102 457 82 441 3.74e-21 95.9
Msa0396000 MtrunA17_Chr6g0486981 24.731 465 279 19 20 457 17 437 7.98e-21 94.7
Msa0396000 MtrunA17_Chr7g0221681 26.276 392 223 19 102 457 73 434 1.59e-19 90.9
Msa0396000 MtrunA17_Chr4g0023351 30.078 256 141 12 77 312 29 266 2.31e-19 88.2
Msa0396000 MtrunA17_Chr4g0053721 23.896 385 228 19 102 456 81 430 5.28e-19 89.7
Msa0396000 MtrunA17_Chr1g0179091 26.703 367 207 16 102 457 52 367 8.73e-19 87.8
Msa0396000 MtrunA17_Chr5g0395871 26.374 455 253 23 32 457 24 425 2.33e-18 87.0
Msa0396000 MtrunA17_Chr8g0371351 25.191 393 202 19 107 456 106 449 4.47e-18 86.7
Msa0396000 MtrunA17_Chr8g0352691 30.738 244 139 9 206 437 15 240 5.29e-17 80.9
Msa0396000 MtrunA17_Chr5g0425141 25.132 378 218 13 108 456 108 449 5.88e-17 83.2
Msa0396000 MtrunA17_Chr3g0124421 24.935 385 213 17 108 456 106 450 1.46e-15 79.0
Msa0396000 MtrunA17_Chr4g0061641 26.972 393 234 16 84 457 39 397 1.90e-14 74.7
Msa0396000 MtrunA17_Chr2g0287821 22.418 455 277 17 54 455 11 442 2.61e-14 74.7
Msa0396000 MtrunA17_Chr1g0185661 24.157 356 206 13 146 455 81 418 1.21e-13 73.6
Msa0396000 MtrunA17_Chr1g0185661 23.834 386 233 15 110 455 706 1070 1.19e-11 67.4
Msa0396000 MtrunA17_Chr4g0010951 28.058 278 142 15 102 355 62 305 2.01e-13 72.0
Msa0396000 MtrunA17_Chr2g0287861 22.000 350 218 14 102 416 44 373 1.18e-12 69.7
Msa0396000 MtrunA17_Chr1g0185651 23.844 411 224 18 102 455 57 435 6.57e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0396000 AT5G07030.1 67.245 461 123 7 5 457 15 455 0.0 592
Msa0396000 AT3G54400.1 65.661 431 119 7 30 457 21 425 0.0 561
Msa0396000 AT1G09750.1 50.812 431 187 9 35 456 34 448 1.03e-140 411
Msa0396000 AT5G10770.1 30.995 442 256 14 38 457 60 474 1.78e-51 181
Msa0396000 AT3G61820.1 34.232 371 212 7 99 456 132 483 1.49e-48 173
Msa0396000 AT3G25700.1 31.504 419 243 12 66 456 47 449 5.65e-48 171
Msa0396000 AT1G01300.1 32.791 369 214 9 99 456 139 484 1.87e-47 170
Msa0396000 AT5G10760.1 31.963 438 243 12 38 456 63 464 3.75e-47 169
Msa0396000 AT2G03200.1 30.831 373 211 11 99 449 104 451 2.30e-46 167
Msa0396000 AT3G20015.1 31.978 369 214 9 99 456 128 470 2.85e-44 161
Msa0396000 AT3G18490.1 32.718 379 216 12 89 456 150 500 5.70e-42 155
Msa0396000 AT1G25510.1 29.975 407 234 11 73 456 105 483 1.01e-40 152
Msa0396000 AT1G79720.1 30.729 384 228 14 88 456 122 482 2.27e-39 148
Msa0396000 AT5G45120.1 29.952 414 213 16 102 456 83 478 6.20e-39 147
Msa0396000 AT1G66180.1 29.952 414 238 18 72 457 38 427 4.19e-38 144
Msa0396000 AT3G52500.1 30.534 393 222 14 102 457 90 468 3.45e-37 142
Msa0396000 AT5G37540.1 29.369 412 235 18 77 456 51 438 3.30e-36 139
Msa0396000 AT2G39710.1 29.534 386 215 16 101 452 64 426 6.24e-34 132
Msa0396000 AT2G42980.1 29.703 404 229 15 85 456 144 524 7.55e-33 130
Msa0396000 AT3G59080.1 28.289 456 243 16 63 456 99 532 4.33e-32 129
Msa0396000 AT1G64830.1 28.442 443 259 17 36 457 24 429 2.26e-31 125
Msa0396000 AT5G22850.1 25.513 439 256 20 57 457 36 441 3.79e-28 117
Msa0396000 AT1G08210.1 27.123 424 266 17 57 457 38 441 1.12e-27 115
Msa0396000 AT1G08210.4 27.123 424 266 17 57 457 72 475 1.27e-27 115
Msa0396000 AT1G08210.3 27.123 424 266 17 57 457 86 489 1.34e-27 115
Msa0396000 AT5G33340.1 26.777 422 264 14 37 443 30 421 1.53e-27 114
Msa0396000 AT5G02190.1 27.895 380 221 16 111 456 82 442 3.42e-27 114
Msa0396000 AT4G30030.1 27.778 396 233 17 78 456 60 419 6.79e-27 112
Msa0396000 AT4G30040.1 27.696 408 243 16 64 449 48 425 1.07e-26 112
Msa0396000 AT3G12700.1 28.235 425 257 18 56 457 62 461 1.34e-26 112
Msa0396000 AT1G08210.2 28.647 377 230 15 57 411 38 397 1.73e-25 109
Msa0396000 AT3G02740.1 26.714 423 242 19 67 457 54 440 1.78e-25 109
Msa0396000 AT3G59080.2 28.409 440 227 16 63 456 99 496 2.50e-25 108
Msa0396000 AT3G02740.2 27.320 388 218 18 102 457 10 365 4.83e-25 107
Msa0396000 AT2G23945.1 25.747 435 253 18 57 456 49 448 4.00e-24 105
Msa0396000 AT4G16563.1 27.804 428 239 15 88 456 75 491 1.52e-23 103
Msa0396000 AT5G43100.1 24.802 379 226 18 102 457 76 418 1.13e-22 101
Msa0396000 AT5G43100.2 24.352 386 233 18 95 457 69 418 2.00e-22 100
Msa0396000 AT1G31450.1 27.212 452 257 20 39 457 30 442 3.59e-22 99.0
Msa0396000 AT2G36670.2 23.371 445 274 19 51 457 43 458 3.74e-22 99.4
Msa0396000 AT2G36670.4 24.227 388 233 18 102 457 34 392 8.22e-22 97.8
Msa0396000 AT2G36670.3 24.227 388 233 18 102 457 34 392 8.22e-22 97.8
Msa0396000 AT2G35615.1 27.254 488 274 23 7 457 1 444 8.51e-22 97.8
Msa0396000 AT2G36670.1 24.227 388 233 18 102 457 105 463 1.25e-21 97.8
Msa0396000 AT2G28040.2 26.902 368 200 17 96 443 50 368 3.21e-21 95.5
Msa0396000 AT3G50050.1 27.297 381 216 19 102 457 93 437 3.66e-21 97.1
Msa0396000 AT4G35880.1 27.493 371 191 18 107 443 112 438 4.64e-21 96.3
Msa0396000 AT2G28040.1 26.902 368 200 17 96 443 59 377 5.06e-21 95.1
Msa0396000 AT2G28030.1 26.140 329 190 11 102 416 61 350 8.88e-21 94.4
Msa0396000 AT5G36260.1 24.093 386 234 17 102 457 78 434 2.18e-20 94.0
Msa0396000 AT2G28010.1 24.100 361 220 12 96 443 59 378 3.52e-19 89.7
Msa0396000 AT4G35880.2 27.577 359 182 18 107 431 112 426 4.22e-19 89.7
Msa0396000 AT2G28225.1 26.486 370 203 17 102 449 67 389 2.26e-18 87.4
Msa0396000 AT1G65240.1 24.000 400 242 19 88 457 60 427 3.40e-17 84.3
Msa0396000 AT3G51350.1 25.888 394 210 18 102 456 102 452 1.11e-16 82.8
Msa0396000 AT2G28220.1 25.698 358 210 13 102 443 67 384 2.15e-16 81.3
Msa0396000 AT3G51340.2 24.615 390 214 18 102 456 103 447 1.91e-14 75.9
Msa0396000 AT3G25700.2 31.016 187 101 7 66 238 47 219 3.63e-14 73.9
Msa0396000 AT3G25700.2 33.588 131 79 2 332 456 219 347 6.65e-12 67.0
Msa0396000 AT2G17760.1 24.439 401 240 19 85 456 83 449 5.59e-14 74.3
Msa0396000 AT5G10080.1 23.776 429 254 17 66 456 60 453 1.28e-13 73.2
Msa0396000 AT3G51360.9 23.706 367 236 14 108 456 105 445 1.61e-13 72.8
Msa0396000 AT3G51360.1 23.497 366 238 13 108 456 95 435 1.85e-13 72.8
Msa0396000 AT3G51360.7 23.497 366 238 13 108 456 11 351 2.08e-13 72.0
Msa0396000 AT4G12920.1 21.351 370 236 13 102 456 58 387 2.33e-13 72.0
Msa0396000 AT4G33490.2 23.650 389 214 18 102 450 60 405 6.36e-13 70.9
Msa0396000 AT4G33490.1 26.596 282 150 15 102 364 57 300 4.22e-12 68.2
Msa0396000 AT3G51340.3 25.617 324 168 16 102 392 103 386 5.32e-12 67.8
Msa0396000 AT1G03220.1 22.646 446 253 17 50 455 24 417 1.02e-11 67.0
Msa0396000 AT1G03230.1 25.714 350 210 17 146 455 79 418 2.47e-11 65.9
Msa0396000 AT3G51340.4 25.161 310 160 14 102 379 103 372 2.50e-11 65.5
Msa0396000 AT3G51340.1 25.484 310 159 14 102 379 103 372 4.36e-11 65.5
Msa0396000 AT3G51360.6 23.729 354 202 16 108 431 105 420 4.41e-11 65.1
Msa0396000 AT3G51360.10 23.754 341 218 13 108 431 95 410 4.57e-11 65.1

Find 169 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTCAAAAGACCAATT+TGG 0.181501 3_2:-13492239 Msa0396000:five_prime_UTR
ATGTTAACTTGGAAGCAATT+AGG 0.215956 3_2:-13489590 Msa0396000:CDS
ATGTGGACTGATAGAGGTTT+TGG 0.221853 3_2:+13489742 None:intergenic
TACATTGTTAGGGCTAAAAT+TGG 0.237202 3_2:-13491865 Msa0396000:CDS
AAAGTCCAACTTACATTGTT+AGG 0.240995 3_2:-13491876 Msa0396000:CDS
ATTTAATCCACACACAAATA+TGG 0.252700 3_2:+13488690 None:intergenic
ATTTAAACCATGAATATAAA+TGG 0.259774 3_2:+13488773 None:intergenic
TCCCATGAAAGTGGCTTTGA+TGG 0.283362 3_2:+13492009 None:intergenic
TTCAAGTCTCTTAACTTCTC+TGG 0.284577 3_2:-13489699 Msa0396000:CDS
AGTGGCTTTGATGGCCTAAA+TGG 0.286534 3_2:+13492018 None:intergenic
TGCCCCACCACAAGGTCTAT+TGG 0.290920 3_2:-13489802 Msa0396000:CDS
TTGTTTGGGTAATGGTAATA+TGG 0.291779 3_2:-13488800 Msa0396000:three_prime_UTR
TTTAACCATGATTACTTCAT+TGG 0.297671 3_2:+13490322 None:intergenic
TGGTACTCCATTTATATTCA+TGG 0.299093 3_2:-13488780 Msa0396000:three_prime_UTR
TAATAGAGAGATGATCTTCT+AGG 0.303672 3_2:+13489612 None:intergenic
ATGGCATTAGAAAAGTTGTT+TGG 0.306401 3_2:-13488815 Msa0396000:three_prime_UTR
TTAGCCCATGTTCCCCATTT+AGG 0.313010 3_2:-13492032 Msa0396000:CDS
TGTTAACTTGGAAGCAATTA+GGG 0.316746 3_2:-13489589 Msa0396000:CDS
TTGAAGTCTAGTTGTGTCTT+TGG 0.320386 3_2:+13491963 None:intergenic
GTTATTGTAGGTACAACTAT+TGG 0.323574 3_2:+13489085 None:intergenic
ACCACAAGGTCTATTGGGCT+TGG 0.324083 3_2:-13489796 Msa0396000:CDS
ATTGTCTTGGACATCACATT+TGG 0.334593 3_2:+13492083 None:intergenic
TCAATTGTACCTATTGCTTC+TGG 0.339113 3_2:-13491913 Msa0396000:CDS
ATCCTCTTGGGCTGAGCAAC+AGG 0.341409 3_2:+13489660 None:intergenic
TTGTCTTGGACATCACATTT+GGG 0.342492 3_2:+13492084 None:intergenic
TTATTTCAAAAGACCAATTT+GGG 0.345523 3_2:-13492238 Msa0396000:five_prime_UTR
TGGGGTTTAGTCCTTGGACT+AGG 0.351891 3_2:+13492103 None:intergenic
TCCACCTCAAACTTTGCTTT+TGG 0.353103 3_2:-13491839 Msa0396000:CDS
CACATTTGGGGTTTAGTCCT+TGG 0.354918 3_2:+13492097 None:intergenic
GCCCCACCACAAGGTCTATT+GGG 0.360432 3_2:-13489801 Msa0396000:CDS
TGGTTGTTATGGTGTGGTTT+TGG 0.362308 3_2:+13492163 None:intergenic
TGTGAGTTTCATGGTTGTTA+TGG 0.369294 3_2:+13492152 None:intergenic
GCCGGTACCATCATTGATTC+AGG 0.372189 3_2:-13489507 Msa0396000:intron
TTGTGTCTTTGGCTTGCATT+TGG 0.373872 3_2:+13491974 None:intergenic
AGGGGTATATTTAATCCTCT+TGG 0.381907 3_2:+13489647 None:intergenic
CCGAATGTGTAACTAGGAAC+TGG 0.388169 3_2:+13489852 None:intergenic
TAGAAAAGTTGTTTGGGTAA+TGG 0.389268 3_2:-13488808 Msa0396000:three_prime_UTR
TGGCATTAGAAAAGTTGTTT+GGG 0.395633 3_2:-13488814 Msa0396000:three_prime_UTR
TTATTGTAGGTACAACTATT+GGG 0.398273 3_2:+13489086 None:intergenic
GAAAGGGCAAAGACCCAAAT+TGG 0.401759 3_2:+13492225 None:intergenic
TCAAGTCTCTTAACTTCTCT+GGG 0.406816 3_2:-13489698 Msa0396000:CDS
CAACACCGAGTTCACATTAT+CGG 0.406860 3_2:+13488964 None:intergenic
AGGGATGTCACTGTGAGCTT+TGG 0.406981 3_2:+13489136 None:intergenic
GGCCATCAAAGCCACTTTCA+TGG 0.410623 3_2:-13492011 Msa0396000:CDS
GAGGGAAAGTGTGAGTTTCA+TGG 0.412360 3_2:+13492143 None:intergenic
TTCCTCTATTGCAGCTAACT+TGG 0.417975 3_2:-13489904 Msa0396000:CDS
TTCTCTGGGTCTTTGAGACT+TGG 0.418986 3_2:-13489684 Msa0396000:CDS
GGTCTATTGGGCTTGGGCCG+AGG 0.426354 3_2:-13489789 Msa0396000:CDS
TCGTGCAAGTTCACGGGTCA+TGG 0.436133 3_2:-13488516 Msa0396000:three_prime_UTR
CGCAGGCAGCACCACGTGTT+TGG 0.436406 3_2:-13489002 Msa0396000:CDS
GGATTAAACGCCAATGCGGC+TGG 0.437790 3_2:+13489540 None:intergenic
CTTGGACTAGGGAGAGGAAT+AGG 0.438297 3_2:+13492115 None:intergenic
TACTATATATAGCAAAAGAA+AGG 0.439490 3_2:+13492208 None:intergenic
ATCTCTCTATTATGTTAACT+TGG 0.439622 3_2:-13489601 Msa0396000:CDS
GCAACACCAACCCTAGAGTT+AGG 0.441048 3_2:+13488890 None:intergenic
GGGGTATATTTAATCCTCTT+GGG 0.444032 3_2:+13489648 None:intergenic
AACACCGAGTTCACATTATC+GGG 0.450137 3_2:+13488965 None:intergenic
TCATTGATGTTCCTAACTCT+AGG 0.450603 3_2:-13488901 Msa0396000:CDS
TTTAATCCACACACAAATAT+GGG 0.453933 3_2:+13488691 None:intergenic
TGGCAGTCAGAGATGAGTTC+CGG 0.456170 3_2:-13489171 Msa0396000:CDS
AAGTCCAACTTACATTGTTA+GGG 0.456447 3_2:-13491875 Msa0396000:CDS
GTGGCTTTGATGGCCTAAAT+GGG 0.457090 3_2:+13492019 None:intergenic
ATGAGTTCCGGCGAAGAGTT+GGG 0.462368 3_2:-13489159 Msa0396000:CDS
CCACAAGGTCTATTGGGCTT+GGG 0.462842 3_2:-13489795 Msa0396000:CDS
GGGGTTTAGTCCTTGGACTA+GGG 0.468206 3_2:+13492104 None:intergenic
TACCAAGTTAGCTGCAATAG+AGG 0.469055 3_2:+13489902 None:intergenic
TGTCTTTGGCTTGCATTTGG+AGG 0.469936 3_2:+13491977 None:intergenic
TGCTGCTCCTGAATGCAAAC+AGG 0.470735 3_2:-13491707 Msa0396000:intron
CCAGTTCCTAGTTACACATT+CGG 0.472772 3_2:-13489852 Msa0396000:CDS
CATTGATGTTCCTAACTCTA+GGG 0.472866 3_2:-13488900 Msa0396000:CDS
ATTATCGGGCGCGGCTGCCA+TGG 0.475957 3_2:+13488979 None:intergenic
TAATTAATCGTGCAAGTTCA+CGG 0.477436 3_2:-13488523 Msa0396000:three_prime_UTR
TGCCTGCGGTGCTATGTATA+AGG 0.481118 3_2:+13489017 None:intergenic
TAGAGGAACTGCCATAGGTT+AGG 0.483289 3_2:+13489919 None:intergenic
ATTCCTTGCACTGCTTGTGA+TGG 0.484236 3_2:-13491787 Msa0396000:CDS
TTCTAGCTACTAAACTATCA+AGG 0.484499 3_2:+13491938 None:intergenic
TGCAATTTCAACCTAACCTA+TGG 0.486060 3_2:-13489930 Msa0396000:CDS
GTATGAATATGCAGCTCTGC+TGG 0.488395 3_2:-13488446 Msa0396000:three_prime_UTR
AGGCAAAGGTTCACGAGACT+TGG 0.489133 3_2:-13490373 Msa0396000:CDS
AGGAACTGGGTCAGTGGCTA+AGG 0.490095 3_2:+13489866 None:intergenic
CATGAGCTCACACCACAACT+AGG 0.490130 3_2:+13489951 None:intergenic
AGCATGTGTCAAACCCTCCT+AGG 0.490363 3_2:+13489116 None:intergenic
GCTAGTTGCACCGGCCTATG+TGG 0.492741 3_2:-13489191 Msa0396000:CDS
TTAGCCCTAACAATGTAAGT+TGG 0.493596 3_2:+13491871 None:intergenic
GCCATCAAAGCCACTTTCAT+GGG 0.495343 3_2:-13492010 Msa0396000:CDS
AATTAATCGTGCAAGTTCAC+GGG 0.495787 3_2:-13488522 Msa0396000:three_prime_UTR
TTTGAGACAAAAGTGACAAC+GGG 0.499233 3_2:+13489766 None:intergenic
GAGCTATGTACTTAATAAGA+TGG 0.501515 3_2:-13488863 Msa0396000:exon
GATGAGTTCCGGCGAAGAGT+TGG 0.502728 3_2:-13489160 Msa0396000:CDS
AAGAGAATGTGGACTGATAG+AGG 0.503256 3_2:+13489736 None:intergenic
GATGTTCCTAACTCTAGGGT+TGG 0.503653 3_2:-13488896 Msa0396000:CDS
CGATATTCCTCCAGCCGCAT+TGG 0.507071 3_2:-13489550 Msa0396000:CDS
ACAAAAGTGACAACGGGCCT+CGG 0.507840 3_2:+13489772 None:intergenic
GATGGCCTAAATGGGGAACA+TGG 0.508158 3_2:+13492027 None:intergenic
CAGCACCACGTGTTTGGCCA+TGG 0.509138 3_2:-13488996 Msa0396000:CDS
ACTATATATAGCAAAAGAAA+GGG 0.509589 3_2:+13492209 None:intergenic
TAGACCTTGTGGTGGGGCAG+AGG 0.509813 3_2:+13489806 None:intergenic
GAGAACATGAATGTTATTGT+AGG 0.510584 3_2:+13489073 None:intergenic
TTCTAGGGTTCTTGAGAAGA+GGG 0.513267 3_2:+13489628 None:intergenic
ATCTGTCTACCAGAAGCAAT+AGG 0.513295 3_2:+13491904 None:intergenic
ACACTTTCTTCCCATGAAAG+TGG 0.514469 3_2:+13492000 None:intergenic
AACTTGGAAGCAATTAGGGT+TGG 0.515766 3_2:-13489585 Msa0396000:CDS
TACCAGGTACCGTGTTTACT+CGG 0.522295 3_2:-13489213 Msa0396000:intron
TTGGCGTTTAATCCGACCAC+TGG 0.524180 3_2:-13489531 Msa0396000:CDS
TGGTGGGGCAGAGGTGCCTG+TGG 0.527039 3_2:+13489815 None:intergenic
ACAATAACATTCATGTTCTC+CGG 0.529103 3_2:-13489070 Msa0396000:CDS
AATAGAGAGATGATCTTCTA+GGG 0.533715 3_2:+13489613 None:intergenic
CTTCTAGGGTTCTTGAGAAG+AGG 0.543537 3_2:+13489627 None:intergenic
ATGGTACCGGCTCCAGTGGT+CGG 0.547154 3_2:+13489519 None:intergenic
TTAGTCCTTGGACTAGGGAG+AGG 0.549316 3_2:+13492109 None:intergenic
ACCTGAATCAATGATGGTAC+CGG 0.549370 3_2:+13489506 None:intergenic
CTCACACCACAACTAGGGTT+AGG 0.549658 3_2:+13489957 None:intergenic
GTTTACTCGGCTAGTTGCAC+CGG 0.552558 3_2:-13489200 Msa0396000:CDS
TCTCTGACTGCCACATAGGC+CGG 0.556988 3_2:+13489181 None:intergenic
ATACTAGTAATGATGCTGCT+TGG 0.557490 3_2:-13491810 Msa0396000:CDS
CGCGCCCGATAATGTGAACT+CGG 0.559273 3_2:-13488969 Msa0396000:CDS
CTATTCCTCTCCCTAGTCCA+AGG 0.561125 3_2:-13492114 Msa0396000:CDS
GGAGTGTTGAACCATTGTCT+TGG 0.564206 3_2:+13492070 None:intergenic
AAGCTCACAGTGACATCCCT+AGG 0.564717 3_2:-13489133 Msa0396000:CDS
AGCCCAATAGACCTTGTGGT+GGG 0.567593 3_2:+13489799 None:intergenic
TGTCTTGGACATCACATTTG+GGG 0.569858 3_2:+13492085 None:intergenic
TTTAATCCGACCACTGGAGC+CGG 0.572128 3_2:-13489525 Msa0396000:CDS
TAATGGTGAGTGTGTGTCAC+GGG 0.573160 3_2:-13488626 Msa0396000:three_prime_UTR
AAGTTAAGAGACTTGAAACT+TGG 0.573810 3_2:+13489705 None:intergenic
CAGGTACCTAACCCTAGTTG+TGG 0.574866 3_2:-13489963 Msa0396000:CDS
GCATGTGTCAAACCCTCCTA+GGG 0.576135 3_2:+13489117 None:intergenic
GGGAGAGGAATAGGAACAAG+AGG 0.576994 3_2:+13492124 None:intergenic
ACACAACCGAATGTGTAACT+AGG 0.578170 3_2:+13489846 None:intergenic
ATAGCCAAAAGCAAAGTTTG+AGG 0.580224 3_2:+13491835 None:intergenic
TGGCAATGTCACGTTCATGC+CGG 0.582971 3_2:+13489051 None:intergenic
GCTTTGGCCCAACTCTTCGC+CGG 0.583717 3_2:+13489152 None:intergenic
CGAATGTGTAACTAGGAACT+GGG 0.585023 3_2:+13489853 None:intergenic
GGCTGCCATGGCCAAACACG+TGG 0.586913 3_2:+13488991 None:intergenic
CAAACACGTGGTGCTGCCTG+CGG 0.588001 3_2:+13489003 None:intergenic
GGTGTTACCTGAATCAATGA+TGG 0.588395 3_2:+13489500 None:intergenic
GCCCAATAGACCTTGTGGTG+GGG 0.592501 3_2:+13489800 None:intergenic
ATCCTTATACATAGCACCGC+AGG 0.593768 3_2:-13489019 Msa0396000:CDS
GTTTCATGGTTGTTATGGTG+TGG 0.593947 3_2:+13492157 None:intergenic
GACCTGTTGCTCAGCCCAAG+AGG 0.602178 3_2:-13489662 Msa0396000:CDS
TGGCAAGCAGTAAGAGAATG+TGG 0.603970 3_2:+13489725 None:intergenic
AATGATGGTACCGGCTCCAG+TGG 0.605589 3_2:+13489515 None:intergenic
CTCATCTCTGACTGCCACAT+AGG 0.606703 3_2:+13489177 None:intergenic
AAATGTGATGTCCAAGACAA+TGG 0.606798 3_2:-13492081 Msa0396000:CDS
GGCACCTCTGCCCCACCACA+AGG 0.606822 3_2:-13489810 Msa0396000:CDS
GCCAAAAGCAAAGTTTGAGG+TGG 0.608306 3_2:+13491838 None:intergenic
CAAGCTAGCTAGCTAGCTAG+AGG 0.610136 3_2:+13488595 None:intergenic
TGCAATAGAGGAACTGCCAT+AGG 0.614874 3_2:+13489914 None:intergenic
ATGAGCTCACACCACAACTA+GGG 0.616988 3_2:+13489952 None:intergenic
AAGCCCAATAGACCTTGTGG+TGG 0.617029 3_2:+13489798 None:intergenic
GTAATGGTGAGTGTGTGTCA+CGG 0.620572 3_2:-13488627 Msa0396000:three_prime_UTR
CAACCATCACAAGCAGTGCA+AGG 0.621327 3_2:+13491784 None:intergenic
CCCAAGCCCAATAGACCTTG+TGG 0.625674 3_2:+13489795 None:intergenic
AGAACTCCCATATTTGTGTG+TGG 0.625885 3_2:-13488697 Msa0396000:three_prime_UTR
ATGGCCTAAATGGGGAACAT+GGG 0.626148 3_2:+13492028 None:intergenic
CGAGTTCACATTATCGGGCG+CGG 0.628806 3_2:+13488970 None:intergenic
CTCACAGTGACATCCCTAGG+AGG 0.629537 3_2:-13489130 Msa0396000:CDS
GTAACTAGGAACTGGGTCAG+TGG 0.631308 3_2:+13489860 None:intergenic
TGCAACTAGCCGAGTAAACA+CGG 0.637706 3_2:+13489204 None:intergenic
TTAAACGCCAATGCGGCTGG+AGG 0.639061 3_2:+13489543 None:intergenic
GGAGAGGAATAGGAACAAGA+GGG 0.641689 3_2:+13492125 None:intergenic
ACTCAAAATCTTACACCATG+TGG 0.647072 3_2:+13488721 None:intergenic
TCACAGTGACATCCCTAGGA+GGG 0.649521 3_2:-13489129 Msa0396000:CDS
AAGGTTTGATTCTAACCACA+TGG 0.650604 3_2:-13488736 Msa0396000:three_prime_UTR
TCTAGGGTTCTTGAGAAGAG+GGG 0.652701 3_2:+13489629 None:intergenic
TGGCTTTGATGGCCTAAATG+GGG 0.655766 3_2:+13492020 None:intergenic
GTTTGAGACAAAAGTGACAA+CGG 0.666616 3_2:+13489765 None:intergenic
TGTATAAGGATGTTGTCTTG+TGG 0.694390 3_2:+13489031 None:intergenic
GAACATCAATGAGCACACGA+TGG 0.713931 3_2:+13488912 None:intergenic
GGTCGGATTAAACGCCAATG+CGG 0.727963 3_2:+13489536 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTACAATTTTTTATGA+AGG + chr3_2:13489376-13489395 None:intergenic 10.0%
!!! ATTAATTTGAAGAATTTAAT+TGG + chr3_2:13489852-13489871 None:intergenic 10.0%
!! AATTAAATTCTTCAAATTAA+TGG - chr3_2:13489851-13489870 Msa0396000:CDS 10.0%
!!! TCAACTTAGATTTTTAAATT+GGG + chr3_2:13489223-13489242 None:intergenic 15.0%
!!! ATCAACTTAGATTTTTAAAT+TGG + chr3_2:13489224-13489243 None:intergenic 15.0%
!! AAAAATGTTAATGAAGAAAA+AGG + chr3_2:13489297-13489316 None:intergenic 15.0%
!!! TTCTTCATTAACATTTTTTT+CGG - chr3_2:13489299-13489318 Msa0396000:intron 15.0%
!! AATTATTAGAATAAATACCT+TGG + chr3_2:13489345-13489364 None:intergenic 15.0%
!! TACTATTGTAGTAAAATTTA+GGG + chr3_2:13489891-13489910 None:intergenic 15.0%
!! GCTATTATATATCATAAAAT+AGG - chr3_2:13489975-13489994 Msa0396000:intron 15.0%
!! ATTACTAATTTCATAATGAA+AGG - chr3_2:13490117-13490136 Msa0396000:intron 15.0%
!! TTACTAATTTCATAATGAAA+GGG - chr3_2:13490118-13490137 Msa0396000:intron 15.0%
!!! AAATGTTTTAAAATTGAGAT+AGG + chr3_2:13490584-13490603 None:intergenic 15.0%
!!! ATTTTGTTTCATTTAAACAT+TGG - chr3_2:13490623-13490642 Msa0396000:intron 15.0%
!!! TTTATTTTGTTTCATTTTTC+AGG - chr3_2:13490661-13490680 Msa0396000:intron 15.0%
!!! CTAATAGTATATTTTTATGT+GGG + chr3_2:13491181-13491200 None:intergenic 15.0%
!!! ACTAATAGTATATTTTTATG+TGG + chr3_2:13491182-13491201 None:intergenic 15.0%
!! ATTTAAACCATGAATATAAA+TGG + chr3_2:13491873-13491892 None:intergenic 15.0%
!! ACTATATATAGCAAAAGAAA+GGG + chr3_2:13488437-13488456 None:intergenic 20.0%
!! TACTATATATAGCAAAAGAA+AGG + chr3_2:13488438-13488457 None:intergenic 20.0%
!!! TTAAATGTAGTTGATTTTTC+AGG + chr3_2:13488904-13488923 None:intergenic 20.0%
!!! TTGTTTTCGTGTTTTTATAT+TGG - chr3_2:13489003-13489022 Msa0396000:CDS 20.0%
!!! TTTTTGTCAAAAAATTACGA+AGG - chr3_2:13489083-13489102 Msa0396000:CDS 20.0%
!! AAATGATGCAATTGTAATTA+TGG - chr3_2:13489139-13489158 Msa0396000:CDS 20.0%
!!! CAACTTAGATTTTTAAATTG+GGG + chr3_2:13489222-13489241 None:intergenic 20.0%
!! AAAAAAAATATGTAAGAGCT+TGG + chr3_2:13489255-13489274 None:intergenic 20.0%
!! TTATATGCAAAATCTATTCA+TGG - chr3_2:13489407-13489426 Msa0396000:intron 20.0%
!!! ACATAAAAATTTGTTTGAAC+TGG - chr3_2:13489751-13489770 Msa0396000:CDS 20.0%
!!! AATAGTGAAAGATTTGTTTT+GGG - chr3_2:13489779-13489798 Msa0396000:CDS 20.0%
!! GTACTATTGTAGTAAAATTT+AGG + chr3_2:13489892-13489911 None:intergenic 20.0%
!!! ATATTTTGGATTCATAGATT+GGG + chr3_2:13490226-13490245 None:intergenic 20.0%
!!! AATATTTTGGATTCATAGAT+TGG + chr3_2:13490227-13490246 None:intergenic 20.0%
!! GAATAAACCAATTGATAAAT+CGG + chr3_2:13490391-13490410 None:intergenic 20.0%
!! AAAGAATCATGATATGATTA+TGG + chr3_2:13491392-13491411 None:intergenic 20.0%
!! ATATATTGTTAAACATTACC+AGG - chr3_2:13491414-13491433 Msa0396000:intron 20.0%
!!! TGGTTTAAATTTTTAGCATA+TGG - chr3_2:13491883-13491902 Msa0396000:CDS 20.0%
!!! TAAATTTTTAGCATATGGTA+AGG - chr3_2:13491888-13491907 Msa0396000:CDS 20.0%
!!! TATATTTGTAGCTTTTGTAA+TGG - chr3_2:13492000-13492019 Msa0396000:CDS 20.0%
!!! TAACTTTGTCACTTTAAAAA+TGG - chr3_2:13492235-13492254 Msa0396000:five_prime_UTR 20.0%
!!! TTTTCGTGTTTTTATATTGG+CGG - chr3_2:13489006-13489025 Msa0396000:CDS 25.0%
!!! AATGAGTTATTTTTACTCCA+AGG - chr3_2:13489325-13489344 Msa0396000:intron 25.0%
!! TGTCGAATATTTGTTGAAAA+AGG - chr3_2:13489485-13489504 Msa0396000:intron 25.0%
!!! CAATAGTGAAAGATTTGTTT+TGG - chr3_2:13489778-13489797 Msa0396000:CDS 25.0%
!!! ATAGTGAAAGATTTGTTTTG+GGG - chr3_2:13489780-13489799 Msa0396000:CDS 25.0%
! TACTACAATAGTACAATACT+TGG - chr3_2:13489898-13489917 Msa0396000:CDS 25.0%
!! CTTTTGTCCCATATTATTAT+AGG - chr3_2:13490180-13490199 Msa0396000:intron 25.0%
!! TTTTTCCAATGAAGTAATCA+TGG - chr3_2:13490316-13490335 Msa0396000:intron 25.0%
! TTTAACCATGATTACTTCAT+TGG + chr3_2:13490324-13490343 None:intergenic 25.0%
!! TAATTGACCGATTTATCAAT+TGG - chr3_2:13490381-13490400 Msa0396000:intron 25.0%
! TCAAAAATTGACCAATACAT+TGG - chr3_2:13490483-13490502 Msa0396000:intron 25.0%
! AAATAAACCAACCAATGTAT+TGG + chr3_2:13490497-13490516 None:intergenic 25.0%
!!! ATACATTGGTTGGTTTATTT+TGG - chr3_2:13490497-13490516 Msa0396000:intron 25.0%
! ATCTCTCTATTATGTTAACT+TGG - chr3_2:13491042-13491061 Msa0396000:intron 25.0%
! TTATTGTAGGTACAACTATT+GGG + chr3_2:13491560-13491579 None:intergenic 25.0%
!! CACATAATCTTTTGTCAATA+TGG - chr3_2:13491809-13491828 Msa0396000:CDS 25.0%
! TTTAATCCACACACAAATAT+GGG + chr3_2:13491955-13491974 None:intergenic 25.0%
! ATTTAATCCACACACAAATA+TGG + chr3_2:13491956-13491975 None:intergenic 25.0%
! TTCTAGCTACTAAACTATCA+AGG + chr3_2:13488708-13488727 None:intergenic 30.0%
AAAGTCCAACTTACATTGTT+AGG - chr3_2:13488767-13488786 Msa0396000:three_prime_UTR 30.0%
AAGTCCAACTTACATTGTTA+GGG - chr3_2:13488768-13488787 Msa0396000:three_prime_UTR 30.0%
TACATTGTTAGGGCTAAAAT+TGG - chr3_2:13488778-13488797 Msa0396000:three_prime_UTR 30.0%
!!! TTTTTTACCTGTTTGCATTC+AGG + chr3_2:13488946-13488965 None:intergenic 30.0%
GACAAAATAACGAGTATCTT+TGG + chr3_2:13489631-13489650 None:intergenic 30.0%
TTTGTACATTGTGACATTTG+AGG + chr3_2:13489696-13489715 None:intergenic 30.0%
AATTAGTAATTACCCTTCCA+CGG + chr3_2:13490107-13490126 None:intergenic 30.0%
TTGTCCCATATTATTATAGG+TGG - chr3_2:13490183-13490202 Msa0396000:intron 30.0%
TGTCCCATATTATTATAGGT+GGG - chr3_2:13490184-13490203 Msa0396000:intron 30.0%
AACCCCACCTATAATAATAT+GGG + chr3_2:13490190-13490209 None:intergenic 30.0%
! GCACATAGCTTTCAATATTT+TGG + chr3_2:13490240-13490259 None:intergenic 30.0%
! ATCAATTGGTTTATTCTCGA+TGG - chr3_2:13490395-13490414 Msa0396000:intron 30.0%
TAGATTGAAATGAGTCGAAT+AGG - chr3_2:13490436-13490455 Msa0396000:intron 30.0%
AAATTGACCAATACATTGGT+TGG - chr3_2:13490487-13490506 Msa0396000:intron 30.0%
!!! GGTTTATTTTGGTCTAACAA+TGG - chr3_2:13490508-13490527 Msa0396000:intron 30.0%
GAGACTTATCATGATTCAAT+TGG + chr3_2:13490548-13490567 None:intergenic 30.0%
AAGTTAAGAGACTTGAAACT+TGG + chr3_2:13490941-13490960 None:intergenic 30.0%
AATAGAGAGATGATCTTCTA+GGG + chr3_2:13491033-13491052 None:intergenic 30.0%
TAATAGAGAGATGATCTTCT+AGG + chr3_2:13491034-13491053 None:intergenic 30.0%
! ATGTTAACTTGGAAGCAATT+AGG - chr3_2:13491053-13491072 Msa0396000:intron 30.0%
! TGTTAACTTGGAAGCAATTA+GGG - chr3_2:13491054-13491073 Msa0396000:intron 30.0%
!! GTATATTTTTATGTGGGAGT+AGG + chr3_2:13491175-13491194 None:intergenic 30.0%
GTTATTGTAGGTACAACTAT+TGG + chr3_2:13491561-13491580 None:intergenic 30.0%
GAGAACATGAATGTTATTGT+AGG + chr3_2:13491573-13491592 None:intergenic 30.0%
ACAATAACATTCATGTTCTC+CGG - chr3_2:13491573-13491592 Msa0396000:intron 30.0%
GAGCTATGTACTTAATAAGA+TGG - chr3_2:13491780-13491799 Msa0396000:CDS 30.0%
ATGGCATTAGAAAAGTTGTT+TGG - chr3_2:13491828-13491847 Msa0396000:CDS 30.0%
TGGCATTAGAAAAGTTGTTT+GGG - chr3_2:13491829-13491848 Msa0396000:CDS 30.0%
TAGAAAAGTTGTTTGGGTAA+TGG - chr3_2:13491835-13491854 Msa0396000:CDS 30.0%
! TTGTTTGGGTAATGGTAATA+TGG - chr3_2:13491843-13491862 Msa0396000:CDS 30.0%
TGGTACTCCATTTATATTCA+TGG - chr3_2:13491863-13491882 Msa0396000:CDS 30.0%
TAATTAATCGTGCAAGTTCA+CGG - chr3_2:13492120-13492139 Msa0396000:CDS 30.0%
TGTGAGTTTCATGGTTGTTA+TGG + chr3_2:13488494-13488513 None:intergenic 35.0%
TTGTCTTGGACATCACATTT+GGG + chr3_2:13488562-13488581 None:intergenic 35.0%
ATTGTCTTGGACATCACATT+TGG + chr3_2:13488563-13488582 None:intergenic 35.0%
AAATGTGATGTCCAAGACAA+TGG - chr3_2:13488562-13488581 Msa0396000:three_prime_UTR 35.0%
TTGAAGTCTAGTTGTGTCTT+TGG + chr3_2:13488683-13488702 None:intergenic 35.0%
TCAATTGTACCTATTGCTTC+TGG - chr3_2:13488730-13488749 Msa0396000:three_prime_UTR 35.0%
TTAGCCCTAACAATGTAAGT+TGG + chr3_2:13488775-13488794 None:intergenic 35.0%
ATAGCCAAAAGCAAAGTTTG+AGG + chr3_2:13488811-13488830 None:intergenic 35.0%
!! TCAAACTTTGCTTTTGGCTA+TGG - chr3_2:13488810-13488829 Msa0396000:three_prime_UTR 35.0%
ATACTAGTAATGATGCTGCT+TGG - chr3_2:13488833-13488852 Msa0396000:three_prime_UTR 35.0%
! ATGTTTTGTCAACTAGCTAG+TGG - chr3_2:13489435-13489454 Msa0396000:intron 35.0%
ATTTCACATGACATACAGCT+TGG - chr3_2:13490058-13490077 Msa0396000:intron 35.0%
TCATAATGAAAGGGACTACA+AGG - chr3_2:13490127-13490146 Msa0396000:intron 35.0%
GTCCCATATTATTATAGGTG+GGG - chr3_2:13490185-13490204 Msa0396000:intron 35.0%
CAACCCCACCTATAATAATA+TGG + chr3_2:13490191-13490210 None:intergenic 35.0%
TGCAATTTCAACCTAACCTA+TGG - chr3_2:13490713-13490732 Msa0396000:intron 35.0%
! TTTGAGACAAAAGTGACAAC+GGG + chr3_2:13490880-13490899 None:intergenic 35.0%
! GTTTGAGACAAAAGTGACAA+CGG + chr3_2:13490881-13490900 None:intergenic 35.0%
TTCAAGTCTCTTAACTTCTC+TGG - chr3_2:13490944-13490963 Msa0396000:intron 35.0%
TCAAGTCTCTTAACTTCTCT+GGG - chr3_2:13490945-13490964 Msa0396000:intron 35.0%
GGGGTATATTTAATCCTCTT+GGG + chr3_2:13490998-13491017 None:intergenic 35.0%
! AGGGGTATATTTAATCCTCT+TGG + chr3_2:13490999-13491018 None:intergenic 35.0%
TTATGTGGGAGTAGGTAAAA+GGG + chr3_2:13491167-13491186 None:intergenic 35.0%
TTTATGTGGGAGTAGGTAAA+AGG + chr3_2:13491168-13491187 None:intergenic 35.0%
TGTATAAGGATGTTGTCTTG+TGG + chr3_2:13491615-13491634 None:intergenic 35.0%
TCATTGATGTTCCTAACTCT+AGG - chr3_2:13491742-13491761 Msa0396000:CDS 35.0%
CATTGATGTTCCTAACTCTA+GGG - chr3_2:13491743-13491762 Msa0396000:CDS 35.0%
AAGGTTTGATTCTAACCACA+TGG - chr3_2:13491907-13491926 Msa0396000:CDS 35.0%
ACTCAAAATCTTACACCATG+TGG + chr3_2:13491925-13491944 None:intergenic 35.0%
AATTAATCGTGCAAGTTCAC+GGG - chr3_2:13492121-13492140 Msa0396000:CDS 35.0%
! TGGTTGTTATGGTGTGGTTT+TGG + chr3_2:13488483-13488502 None:intergenic 40.0%
GTTTCATGGTTGTTATGGTG+TGG + chr3_2:13488489-13488508 None:intergenic 40.0%
TGTCTTGGACATCACATTTG+GGG + chr3_2:13488561-13488580 None:intergenic 40.0%
GGCTAAAAACATGGATCACT+TGG + chr3_2:13488597-13488616 None:intergenic 40.0%
ACACTTTCTTCCCATGAAAG+TGG + chr3_2:13488646-13488665 None:intergenic 40.0%
!! TTGTGTCTTTGGCTTGCATT+TGG + chr3_2:13488672-13488691 None:intergenic 40.0%
ATCTGTCTACCAGAAGCAAT+AGG + chr3_2:13488742-13488761 None:intergenic 40.0%
! TCCACCTCAAACTTTGCTTT+TGG - chr3_2:13488804-13488823 Msa0396000:three_prime_UTR 40.0%
! TCTTTTACCTTTACTTGCCG+TGG - chr3_2:13490087-13490106 Msa0396000:intron 40.0%
TGAAAGCTATGTGCTCTCTA+TGG - chr3_2:13490246-13490265 Msa0396000:intron 40.0%
ATAGACGCTGTGGGATAAAT+CGG - chr3_2:13490351-13490370 Msa0396000:CDS 40.0%
TTCCTCTATTGCAGCTAACT+TGG - chr3_2:13490739-13490758 Msa0396000:intron 40.0%
TACCAAGTTAGCTGCAATAG+AGG + chr3_2:13490744-13490763 None:intergenic 40.0%
CGAATGTGTAACTAGGAACT+GGG + chr3_2:13490793-13490812 None:intergenic 40.0%
CCAGTTCCTAGTTACACATT+CGG - chr3_2:13490791-13490810 Msa0396000:intron 40.0%
ACACAACCGAATGTGTAACT+AGG + chr3_2:13490800-13490819 None:intergenic 40.0%
GGTTGTGTTTCAAAAACCAC+AGG - chr3_2:13490812-13490831 Msa0396000:intron 40.0%
!! TGTGGACTGATAGAGGTTTT+GGG + chr3_2:13490903-13490922 None:intergenic 40.0%
!! ATGTGGACTGATAGAGGTTT+TGG + chr3_2:13490904-13490923 None:intergenic 40.0%
! AAGAGAATGTGGACTGATAG+AGG + chr3_2:13490910-13490929 None:intergenic 40.0%
!! TTCTAGGGTTCTTGAGAAGA+GGG + chr3_2:13491018-13491037 None:intergenic 40.0%
AACTTGGAAGCAATTAGGGT+TGG - chr3_2:13491058-13491077 Msa0396000:intron 40.0%
! GAGGAATATCGACGACTTTT+CGG + chr3_2:13491084-13491103 None:intergenic 40.0%
ACCTGAATCAATGATGGTAC+CGG + chr3_2:13491140-13491159 None:intergenic 40.0%
! GGTGTTACCTGAATCAATGA+TGG + chr3_2:13491146-13491165 None:intergenic 40.0%
AACACCGAGTTCACATTATC+GGG + chr3_2:13491681-13491700 None:intergenic 40.0%
CAACACCGAGTTCACATTAT+CGG + chr3_2:13491682-13491701 None:intergenic 40.0%
!!! ATGGTTTTGTTGCTGCATGT+TGG + chr3_2:13491715-13491734 None:intergenic 40.0%
AGAACTCCCATATTTGTGTG+TGG - chr3_2:13491946-13491965 Msa0396000:CDS 40.0%
GAAAGGGCAAAGACCCAAAT+TGG + chr3_2:13488421-13488440 None:intergenic 45.0%
! GAGGGAAAGTGTGAGTTTCA+TGG + chr3_2:13488503-13488522 None:intergenic 45.0%
GGAGAGGAATAGGAACAAGA+GGG + chr3_2:13488521-13488540 None:intergenic 45.0%
! CACATTTGGGGTTTAGTCCT+TGG + chr3_2:13488549-13488568 None:intergenic 45.0%
!! GGAGTGTTGAACCATTGTCT+TGG + chr3_2:13488576-13488595 None:intergenic 45.0%
! GGGAACATGGGCTAAAAACA+TGG + chr3_2:13488606-13488625 None:intergenic 45.0%
TTAGCCCATGTTCCCCATTT+AGG - chr3_2:13488611-13488630 Msa0396000:three_prime_UTR 45.0%
ATGGCCTAAATGGGGAACAT+GGG + chr3_2:13488618-13488637 None:intergenic 45.0%
!! TGGCTTTGATGGCCTAAATG+GGG + chr3_2:13488626-13488645 None:intergenic 45.0%
!! GTGGCTTTGATGGCCTAAAT+GGG + chr3_2:13488627-13488646 None:intergenic 45.0%
!! AGTGGCTTTGATGGCCTAAA+TGG + chr3_2:13488628-13488647 None:intergenic 45.0%
! GCCATCAAAGCCACTTTCAT+GGG - chr3_2:13488633-13488652 Msa0396000:three_prime_UTR 45.0%
!! TCCCATGAAAGTGGCTTTGA+TGG + chr3_2:13488637-13488656 None:intergenic 45.0%
!! TGTCTTTGGCTTGCATTTGG+AGG + chr3_2:13488669-13488688 None:intergenic 45.0%
GCCAAAAGCAAAGTTTGAGG+TGG + chr3_2:13488808-13488827 None:intergenic 45.0%
ATTCCTTGCACTGCTTGTGA+TGG - chr3_2:13488856-13488875 Msa0396000:exon 45.0%
TTACCTTTACTTGCCGTGGA+AGG - chr3_2:13490091-13490110 Msa0396000:intron 45.0%
TACCTTTACTTGCCGTGGAA+GGG - chr3_2:13490092-13490111 Msa0396000:intron 45.0%
AAAGAGTTCGATAGACGCTG+TGG - chr3_2:13490341-13490360 Msa0396000:CDS 45.0%
AAGAGTTCGATAGACGCTGT+GGG - chr3_2:13490342-13490361 Msa0396000:CDS 45.0%
ATGAGCTCACACCACAACTA+GGG + chr3_2:13490694-13490713 None:intergenic 45.0%
TAGAGGAACTGCCATAGGTT+AGG + chr3_2:13490727-13490746 None:intergenic 45.0%
TGCAATAGAGGAACTGCCAT+AGG + chr3_2:13490732-13490751 None:intergenic 45.0%
CCGAATGTGTAACTAGGAAC+TGG + chr3_2:13490794-13490813 None:intergenic 45.0%
TGGCAAGCAGTAAGAGAATG+TGG + chr3_2:13490921-13490940 None:intergenic 45.0%
!! TTCTCTGGGTCTTTGAGACT+TGG - chr3_2:13490959-13490978 Msa0396000:intron 45.0%
! TCTAGGGTTCTTGAGAAGAG+GGG + chr3_2:13491017-13491036 None:intergenic 45.0%
!! CTTCTAGGGTTCTTGAGAAG+AGG + chr3_2:13491019-13491038 None:intergenic 45.0%
! TACCAGGTACCGTGTTTACT+CGG - chr3_2:13491430-13491449 Msa0396000:intron 45.0%
TGCAACTAGCCGAGTAAACA+CGG + chr3_2:13491442-13491461 None:intergenic 45.0%
ATCCTTATACATAGCACCGC+AGG - chr3_2:13491624-13491643 Msa0396000:intron 45.0%
GAACATCAATGAGCACACGA+TGG + chr3_2:13491734-13491753 None:intergenic 45.0%
!! GATGTTCCTAACTCTAGGGT+TGG - chr3_2:13491747-13491766 Msa0396000:CDS 45.0%
GTAATGGTGAGTGTGTGTCA+CGG - chr3_2:13492016-13492035 Msa0396000:CDS 45.0%
TAATGGTGAGTGTGTGTCAC+GGG - chr3_2:13492017-13492036 Msa0396000:CDS 45.0%
GTATGAATATGCAGCTCTGC+TGG - chr3_2:13492197-13492216 Msa0396000:five_prime_UTR 45.0%
GGGAGAGGAATAGGAACAAG+AGG + chr3_2:13488522-13488541 None:intergenic 50.0%
! CTTGGACTAGGGAGAGGAAT+AGG + chr3_2:13488531-13488550 None:intergenic 50.0%
CTATTCCTCTCCCTAGTCCA+AGG - chr3_2:13488529-13488548 Msa0396000:three_prime_UTR 50.0%
! TTAGTCCTTGGACTAGGGAG+AGG + chr3_2:13488537-13488556 None:intergenic 50.0%
GGGGTTTAGTCCTTGGACTA+GGG + chr3_2:13488542-13488561 None:intergenic 50.0%
TGGGGTTTAGTCCTTGGACT+AGG + chr3_2:13488543-13488562 None:intergenic 50.0%
GATGGCCTAAATGGGGAACA+TGG + chr3_2:13488619-13488638 None:intergenic 50.0%
! GGCCATCAAAGCCACTTTCA+TGG - chr3_2:13488632-13488651 Msa0396000:three_prime_UTR 50.0%
CAACCATCACAAGCAGTGCA+AGG + chr3_2:13488862-13488881 None:intergenic 50.0%
TGCTGCTCCTGAATGCAAAC+AGG - chr3_2:13488936-13488955 Msa0396000:CDS 50.0%
TACCCTTCCACGGCAAGTAA+AGG + chr3_2:13490097-13490116 None:intergenic 50.0%
AGCTATGTGCTCTCTATGGC+AGG - chr3_2:13490250-13490269 Msa0396000:intron 50.0%
AGGCAAAGGTTCACGAGACT+TGG - chr3_2:13490270-13490289 Msa0396000:intron 50.0%
CAGGTACCTAACCCTAGTTG+TGG - chr3_2:13490680-13490699 Msa0396000:intron 50.0%
CTCACACCACAACTAGGGTT+AGG + chr3_2:13490689-13490708 None:intergenic 50.0%
CATGAGCTCACACCACAACT+AGG + chr3_2:13490695-13490714 None:intergenic 50.0%
GTAACTAGGAACTGGGTCAG+TGG + chr3_2:13490786-13490805 None:intergenic 50.0%
AGCCCAATAGACCTTGTGGT+GGG + chr3_2:13490847-13490866 None:intergenic 50.0%
AAGCCCAATAGACCTTGTGG+TGG + chr3_2:13490848-13490867 None:intergenic 50.0%
ACCACAAGGTCTATTGGGCT+TGG - chr3_2:13490847-13490866 Msa0396000:intron 50.0%
CCACAAGGTCTATTGGGCTT+GGG - chr3_2:13490848-13490867 Msa0396000:intron 50.0%
ACAAAAGTGACAACGGGCCT+CGG + chr3_2:13490874-13490893 None:intergenic 50.0%
GGTCGGATTAAACGCCAATG+CGG + chr3_2:13491110-13491129 None:intergenic 50.0%
TTGGCGTTTAATCCGACCAC+TGG - chr3_2:13491112-13491131 Msa0396000:intron 50.0%
TTTAATCCGACCACTGGAGC+CGG - chr3_2:13491118-13491137 Msa0396000:intron 50.0%
!! GCCGGTACCATCATTGATTC+AGG - chr3_2:13491136-13491155 Msa0396000:intron 50.0%
! GTTTACTCGGCTAGTTGCAC+CGG - chr3_2:13491443-13491462 Msa0396000:intron 50.0%
CTCATCTCTGACTGCCACAT+AGG + chr3_2:13491469-13491488 None:intergenic 50.0%
TGGCAGTCAGAGATGAGTTC+CGG - chr3_2:13491472-13491491 Msa0396000:intron 50.0%
ATGAGTTCCGGCGAAGAGTT+GGG - chr3_2:13491484-13491503 Msa0396000:intron 50.0%
AGGGATGTCACTGTGAGCTT+TGG + chr3_2:13491510-13491529 None:intergenic 50.0%
AAGCTCACAGTGACATCCCT+AGG - chr3_2:13491510-13491529 Msa0396000:intron 50.0%
TCACAGTGACATCCCTAGGA+GGG - chr3_2:13491514-13491533 Msa0396000:intron 50.0%
GCATGTGTCAAACCCTCCTA+GGG + chr3_2:13491529-13491548 None:intergenic 50.0%
AGCATGTGTCAAACCCTCCT+AGG + chr3_2:13491530-13491549 None:intergenic 50.0%
TGGCAATGTCACGTTCATGC+CGG + chr3_2:13491595-13491614 None:intergenic 50.0%
!! TGCCTGCGGTGCTATGTATA+AGG + chr3_2:13491629-13491648 None:intergenic 50.0%
GCAACACCAACCCTAGAGTT+AGG + chr3_2:13491756-13491775 None:intergenic 50.0%
CAAGCTAGCTAGCTAGCTAG+AGG + chr3_2:13492051-13492070 None:intergenic 50.0%
GTGCTCTCTATGGCAGGCAA+AGG - chr3_2:13490256-13490275 Msa0396000:intron 55.0%
AGGAACTGGGTCAGTGGCTA+AGG + chr3_2:13490780-13490799 None:intergenic 55.0%
TGCCCCACCACAAGGTCTAT+TGG - chr3_2:13490841-13490860 Msa0396000:intron 55.0%
GCCCCACCACAAGGTCTATT+GGG - chr3_2:13490842-13490861 Msa0396000:intron 55.0%
GCCCAATAGACCTTGTGGTG+GGG + chr3_2:13490846-13490865 None:intergenic 55.0%
CCCAAGCCCAATAGACCTTG+TGG + chr3_2:13490851-13490870 None:intergenic 55.0%
ATCCTCTTGGGCTGAGCAAC+AGG + chr3_2:13490986-13491005 None:intergenic 55.0%
CGATATTCCTCCAGCCGCAT+TGG - chr3_2:13491093-13491112 Msa0396000:intron 55.0%
TTAAACGCCAATGCGGCTGG+AGG + chr3_2:13491103-13491122 None:intergenic 55.0%
GGATTAAACGCCAATGCGGC+TGG + chr3_2:13491106-13491125 None:intergenic 55.0%
!! AATGATGGTACCGGCTCCAG+TGG + chr3_2:13491131-13491150 None:intergenic 55.0%
AGCCGAGTAAACACGGTACC+TGG + chr3_2:13491435-13491454 None:intergenic 55.0%
TCTCTGACTGCCACATAGGC+CGG + chr3_2:13491465-13491484 None:intergenic 55.0%
GATGAGTTCCGGCGAAGAGT+TGG - chr3_2:13491483-13491502 Msa0396000:intron 55.0%
CTCACAGTGACATCCCTAGG+AGG - chr3_2:13491513-13491532 Msa0396000:intron 55.0%
CGAGTTCACATTATCGGGCG+CGG + chr3_2:13491676-13491695 None:intergenic 55.0%
CGCGCCCGATAATGTGAACT+CGG - chr3_2:13491674-13491693 Msa0396000:intron 55.0%
TCGTGCAAGTTCACGGGTCA+TGG - chr3_2:13492127-13492146 Msa0396000:CDS 55.0%
! TAGACCTTGTGGTGGGGCAG+AGG + chr3_2:13490840-13490859 None:intergenic 60.0%
GACCTGTTGCTCAGCCCAAG+AGG - chr3_2:13490981-13491000 Msa0396000:intron 60.0%
!! ATGGTACCGGCTCCAGTGGT+CGG + chr3_2:13491127-13491146 None:intergenic 60.0%
! GCTAGTTGCACCGGCCTATG+TGG - chr3_2:13491452-13491471 Msa0396000:intron 60.0%
GCTTTGGCCCAACTCTTCGC+CGG + chr3_2:13491494-13491513 None:intergenic 60.0%
!! CAAACACGTGGTGCTGCCTG+CGG + chr3_2:13491643-13491662 None:intergenic 60.0%
CAGCACCACGTGTTTGGCCA+TGG - chr3_2:13491647-13491666 Msa0396000:intron 60.0%
GGTCTATTGGGCTTGGGCCG+AGG - chr3_2:13490854-13490873 Msa0396000:intron 65.0%
CGCAGGCAGCACCACGTGTT+TGG - chr3_2:13491641-13491660 Msa0396000:intron 65.0%
GGCTGCCATGGCCAAACACG+TGG + chr3_2:13491655-13491674 None:intergenic 65.0%
ATTATCGGGCGCGGCTGCCA+TGG + chr3_2:13491667-13491686 None:intergenic 65.0%
!! TGGTGGGGCAGAGGTGCCTG+TGG + chr3_2:13490831-13490850 None:intergenic 70.0%
GGCACCTCTGCCCCACCACA+AGG - chr3_2:13490833-13490852 Msa0396000:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 13488407 13492258 13488407 ID=Msa0396000;Name=Msa0396000
chr3_2 mRNA 13488407 13492258 13488407 ID=Msa0396000-mRNA-1;Parent=Msa0396000;Name=Msa0396000-mRNA-1;_AED=0.02;_eAED=0.02;_QI=68|0.33|0.75|0.75|1|1|4|464|457
chr3_2 exon 13488407 13489229 13488407 ID=Msa0396000-mRNA-1:exon:3159;Parent=Msa0396000-mRNA-1
chr3_2 exon 13489508 13489982 13489508 ID=Msa0396000-mRNA-1:exon:3158;Parent=Msa0396000-mRNA-1
chr3_2 exon 13490337 13490393 13490337 ID=Msa0396000-mRNA-1:exon:3157;Parent=Msa0396000-mRNA-1
chr3_2 exon 13491708 13492258 13491708 ID=Msa0396000-mRNA-1:exon:3156;Parent=Msa0396000-mRNA-1
chr3_2 five_prime_UTR 13492191 13492258 13492191 ID=Msa0396000-mRNA-1:five_prime_utr;Parent=Msa0396000-mRNA-1
chr3_2 CDS 13491708 13492190 13491708 ID=Msa0396000-mRNA-1:cds;Parent=Msa0396000-mRNA-1
chr3_2 CDS 13490337 13490393 13490337 ID=Msa0396000-mRNA-1:cds;Parent=Msa0396000-mRNA-1
chr3_2 CDS 13489508 13489982 13489508 ID=Msa0396000-mRNA-1:cds;Parent=Msa0396000-mRNA-1
chr3_2 CDS 13488871 13489229 13488871 ID=Msa0396000-mRNA-1:cds;Parent=Msa0396000-mRNA-1
chr3_2 three_prime_UTR 13488407 13488870 13488407 ID=Msa0396000-mRNA-1:three_prime_utr;Parent=Msa0396000-mRNA-1
Gene Sequence

>Msa0396000

ATGAACCAAAACCACACCATAACAACCATGAAACTCACACTTTCCCTCTTGTTCCTATTCCTCTCCCTAGTCCAAGGACTAAACCCCAAATGTGATGTCCAAGACAATGGTTCAACACTCCAAGTGATCCATGTTTTTAGCCCATGTTCCCCATTTAGGCCATCAAAGCCACTTTCATGGGAAGAAAGTGTCCTCCAAATGCAAGCCAAAGACACAACTAGACTTCAATTCCTTGATAGTTTAGTAGCTAGAAAATCAATTGTACCTATTGCTTCTGGTAGACAGATCATACAAAGTCCAACTTACATTGTTAGGGCTAAAATTGGTACTCCACCTCAAACTTTGCTTTTGGCTATGGATACTAGTAATGATGCTGCTTGGATTCCTTGCACTGCTTGTGATGGTTGTGCTTCAACTTTGTTTGCTCCTGAAAAATCAACTACATTTAAGAATGTTAGTTGTGCTGCTCCTGAATGCAAACAGGCAAAGGTTCACGAGACTTGGAGTCTTCTAAAATCTAAATGCACTTTTTCCAATGAAGTACCTAACCCTAGTTGTGGTGTGAGCTCATGCAATTTCAACCTAACCTATGGCAGTTCCTCTATTGCAGCTAACTTGGTACAAGACACAGTTACCTTAGCCACTGACCCAGTTCCTAGTTACACATTCGGTTGTGTTTCAAAAACCACAGGCACCTCTGCCCCACCACAAGGTCTATTGGGCTTGGGCCGAGGCCCGTTGTCACTTTTGTCTCAAACCCAAAACCTCTATCAGTCCACATTCTCTTACTGCTTGCCAAGTTTCAAGTCTCTTAACTTCTCTGGGTCTTTGAGACTTGGACCTGTTGCTCAGCCCAAGAGGATTAAATATACCCCTCTTCTCAAGAACCCTAGAAGATCATCTCTCTATTATGTTAACTTGGAAGCAATTAGGGTTGGCCGAAAAGTCGTCGATATTCCTCCAGCCGCATTGGCGTTTAATCCGACCACTGGAGCCGGTACCATCATTGATTCAGGTACCGTGTTTACTCGGCTAGTTGCACCGGCCTATGTGGCAGTCAGAGATGAGTTCCGGCGAAGAGTTGGGCCAAAGCTCACAGTGACATCCCTAGGAGGGTTTGACACATGCTACAATGTCCCAATAGTTGTACCTACAATAACATTCATGTTCTCCGGCATGAACGTGACATTGCCACAAGACAACATCCTTATACATAGCACCGCAGGCAGCACCACGTGTTTGGCCATGGCAGCCGCGCCCGATAATGTGAACTCGGTGTTGAACGTGATAGCCAACATGCAGCAACAAAACCATCGTGTGCTCATTGATGTTCCTAACTCTAGGGTTGGTGTTGCTCGTGAGCTATGTACTTAA
Protein sequence

>Msa0396000

MNQNHTITTMKLTLSLLFLFLSLVQGLNPKCDVQDNGSTLQVIHVFSPCSPFRPSKPLSWEESVLQMQAKDTTRLQFLDSLVARKSIVPIASGRQIIQSPTYIVRAKIGTPPQTLLLAMDTSNDAAWIPCTACDGCASTLFAPEKSTTFKNVSCAAPECKQAKVHETWSLLKSKCTFSNEVPNPSCGVSSCNFNLTYGSSSIAANLVQDTVTLATDPVPSYTFGCVSKTTGTSAPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSLNFSGSLRLGPVAQPKRIKYTPLLKNPRRSSLYYVNLEAIRVGRKVVDIPPAALAFNPTTGAGTIIDSGTVFTRLVAPAYVAVRDEFRRRVGPKLTVTSLGGFDTCYNVPIVVPTITFMFSGMNVTLPQDNILIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRVLIDVPNSRVGVARELCT*