AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0422250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0422250 MtrunA17_Chr3g0123131 97.544 570 14 0 1 570 1 570 0.0 1165
Msa0422250 MtrunA17_Chr8g0376881 61.431 573 163 10 1 561 12 538 0.0 649
Msa0422250 MtrunA17_Chr1g0161431 56.985 272 110 2 12 278 10 279 1.66e-96 306
Msa0422250 MtrunA17_Chr4g0067981 59.770 261 91 2 13 267 31 283 9.84e-93 298
Msa0422250 MtrunA17_Chr2g0294911 56.917 253 105 1 13 265 29 277 2.33e-90 290
Msa0422250 MtrunA17_Chr3g0137101 52.288 306 115 5 13 290 8 310 4.63e-88 285
Msa0422250 MtrunA17_Chr1g0149841 55.777 251 99 3 13 257 5 249 1.24e-80 261
Msa0422250 MtrunA17_Chr7g0275891 43.385 325 150 6 13 310 5 322 7.97e-76 244
Msa0422250 MtrunA17_Chr2g0304841 51.737 259 104 2 13 256 18 270 3.59e-74 249
Msa0422250 MtrunA17_Chr4g0074411 52.590 251 108 3 13 259 18 261 1.08e-73 246
Msa0422250 MtrunA17_Chr4g0074401 51.351 259 108 5 10 259 14 263 1.55e-72 243
Msa0422250 MtrunA17_Chr1g0149851 51.515 264 103 4 13 256 5 263 3.95e-71 238
Msa0422250 MtrunA17_Chr7g0275401 48.120 266 125 3 12 269 4 264 5.38e-69 224
Msa0422250 MtrunA17_Chr3g0134481 45.798 238 67 2 13 250 8 183 9.15e-60 196
Msa0422250 MtrunA17_Chr4g0009851 39.689 257 127 3 6 262 4 232 1.64e-58 197
Msa0422250 MtrunA17_Chr4g0009901 42.366 262 121 4 13 274 10 241 2.00e-58 196
Msa0422250 MtrunA17_Chr4g0074421 51.269 197 79 5 63 253 2 187 7.70e-54 181
Msa0422250 MtrunA17_Chr1g0149831 52.604 192 76 4 72 257 1 183 2.88e-52 178
Msa0422250 MtrunA17_Chr3g0134461 45.148 237 77 2 13 248 8 192 4.63e-51 174
Msa0422250 MtrunA17_Chr6g0471041 36.327 245 126 5 11 254 12 227 2.33e-43 160
Msa0422250 MtrunA17_Chr4g0009981 38.863 211 102 3 58 268 2 185 1.53e-42 153
Msa0422250 MtrunA17_Chr8g0391911 32.549 255 132 6 13 248 9 242 1.17e-33 129
Msa0422250 MtrunA17_Chr8g0383961 44.094 127 71 0 13 139 14 140 8.52e-29 115
Msa0422250 MtrunA17_Chr8g0350761 31.250 256 154 7 15 251 19 271 1.56e-26 113
Msa0422250 MtrunA17_Chr4g0011071 32.927 164 83 3 59 222 35 171 2.38e-26 105
Msa0422250 MtrunA17_Chr6g0456631 35.507 138 81 4 12 142 8 144 4.26e-24 99.4
Msa0422250 MtrunA17_Chr6g0473731 35.484 186 95 5 93 278 10 170 7.16e-24 104
Msa0422250 MtrunA17_Chr3g0127941 41.129 124 68 2 17 136 56 178 1.43e-23 105
Msa0422250 MtrunA17_Chr7g0276361 37.500 128 71 2 14 135 11 135 6.82e-21 96.7
Msa0422250 MtrunA17_Chr3g0091641 34.959 123 77 1 17 136 24 146 5.55e-20 93.6
Msa0422250 MtrunA17_Chr4g0061021 35.772 123 76 1 17 136 33 155 1.15e-19 92.8
Msa0422250 MtrunA17_Chr4g0030271 36.585 123 73 2 17 135 89 210 1.35e-19 93.2
Msa0422250 MtrunA17_Chr8g0345901 34.586 133 78 3 9 137 32 159 6.21e-19 90.5
Msa0422250 MtrunA17_Chr1g0181811 36.066 122 75 1 17 135 85 206 1.89e-18 89.4
Msa0422250 MtrunA17_Chr4g0009991 35.417 144 65 3 105 248 1 116 2.36e-18 81.3
Msa0422250 MtrunA17_Chr5g0423031 43.210 81 45 1 13 93 10 89 3.42e-18 82.8
Msa0422250 MtrunA17_Chr4g0031961 42.424 99 51 1 176 268 102 200 8.44e-17 81.6
Msa0422250 MtrunA17_Chr4g0010001 44.444 81 44 1 13 93 10 89 1.61e-16 75.1
Msa0422250 MtrunA17_Chr4g0054981 36.508 126 76 2 11 135 12 134 3.64e-16 81.6
Msa0422250 MtrunA17_Chr4g0054981 58.929 56 23 0 198 253 309 364 1.53e-13 73.6
Msa0422250 MtrunA17_Chr7g0223971 27.727 220 100 5 95 278 1 197 3.89e-16 81.3
Msa0422250 MtrunA17_Chr7g0255711 57.377 61 26 0 198 258 90 150 8.30e-16 78.6
Msa0422250 MtrunA17_Chr5g0400241 36.220 127 75 3 11 135 12 134 3.73e-14 75.5
Msa0422250 MtrunA17_Chr5g0400241 47.222 72 38 0 193 264 305 376 4.56e-14 75.1
Msa0422250 MtrunA17_Chr4g0059571 29.078 141 85 3 14 139 20 160 3.62e-13 68.9
Msa0422250 MtrunA17_Chr2g0324891 43.662 71 40 0 198 268 160 230 1.59e-12 69.7
Msa0422250 MtrunA17_Chr4g0040481 43.478 69 39 0 198 266 157 225 2.64e-12 68.9
Msa0422250 MtrunA17_Chr7g0256551 28.571 140 79 2 19 137 11 150 5.49e-12 65.9
Msa0422250 MtrunA17_Chr8g0352871 28.472 144 83 3 19 142 11 154 1.61e-11 63.2
Msa0422250 MtrunA17_Chr6g0461641 35.897 117 54 3 195 296 38 148 3.39e-11 65.5
Msa0422250 MtrunA17_Chr6g0469321 70.455 44 13 0 214 257 69 112 4.61e-11 62.0
Msa0422250 MtrunA17_Chr1g0198601 34.167 120 70 3 167 285 183 294 5.08e-11 64.7
Msa0422250 MtrunA17_Chr2g0321461 49.153 59 29 1 195 252 41 99 5.44e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0422250 AT1G67710.1 51.247 441 149 11 14 438 8 398 2.08e-124 376
Msa0422250 AT3G16857.1 58.582 268 83 4 13 257 33 295 1.12e-90 293
Msa0422250 AT3G16857.2 58.582 268 83 4 13 257 33 295 1.71e-90 293
Msa0422250 AT2G01760.1 54.307 267 113 3 13 271 7 272 1.04e-85 271
Msa0422250 AT1G67710.2 45.170 383 144 11 72 438 1 333 5.77e-83 266
Msa0422250 AT2G25180.1 56.641 256 101 3 13 265 13 261 2.51e-82 269
Msa0422250 AT2G01760.2 41.514 383 169 7 13 344 7 385 1.30e-79 256
Msa0422250 AT4G31920.1 49.242 264 106 4 13 270 13 254 9.69e-75 248
Msa0422250 AT3G16857.3 56.769 229 71 4 52 257 2 225 4.80e-70 237
Msa0422250 AT5G58080.1 49.621 264 126 4 1 263 1 258 2.94e-67 230
Msa0422250 AT1G67710.3 41.873 363 145 11 92 438 12 324 2.96e-66 223
Msa0422250 AT2G01760.3 52.404 208 90 3 72 271 1 207 5.42e-59 199
Msa0422250 AT5G49240.1 40.161 249 133 5 6 248 34 272 4.29e-49 172
Msa0422250 AT1G49190.1 31.047 277 166 5 13 275 30 295 9.58e-37 141
Msa0422250 AT5G07210.2 32.727 275 141 5 17 257 16 280 1.96e-35 141
Msa0422250 AT5G07210.1 31.802 283 141 7 17 257 16 288 3.10e-33 134
Msa0422250 AT3G62670.1 35.833 240 135 6 18 251 42 268 8.08e-33 130
Msa0422250 AT2G27070.1 32.472 271 150 8 17 257 16 283 3.57e-32 131
Msa0422250 AT1G49190.2 41.600 125 73 0 13 137 30 154 7.11e-26 112
Msa0422250 AT1G68210.1 22.843 394 254 6 4 353 3 390 2.04e-22 102
Msa0422250 AT2G46790.3 38.211 123 71 2 17 135 37 158 6.43e-22 99.4
Msa0422250 AT2G46790.1 38.211 123 71 2 17 135 37 158 7.17e-22 99.4
Msa0422250 AT5G24470.1 37.600 125 73 2 16 136 49 172 7.51e-22 99.8
Msa0422250 AT5G60100.2 41.085 129 70 3 17 141 64 190 3.63e-21 97.4
Msa0422250 AT5G61380.1 37.398 123 74 1 17 136 19 141 1.14e-20 96.3
Msa0422250 AT5G60100.7 39.837 123 69 2 17 135 64 185 1.84e-20 94.4
Msa0422250 AT5G60100.6 39.837 123 69 2 17 135 64 185 1.84e-20 94.4
Msa0422250 AT5G60100.4 39.837 123 69 2 17 135 64 185 1.84e-20 94.4
Msa0422250 AT5G60100.5 39.837 123 69 2 17 135 64 185 2.99e-20 94.7
Msa0422250 AT5G60100.3 39.837 123 69 2 17 135 64 185 2.99e-20 94.7
Msa0422250 AT5G60100.1 39.837 123 69 2 17 135 64 185 2.99e-20 94.7
Msa0422250 AT5G59570.2 46.392 97 45 2 162 258 111 200 6.85e-18 85.1
Msa0422250 AT5G59570.1 46.392 97 45 2 162 258 111 200 6.85e-18 85.1
Msa0422250 AT5G02810.1 37.398 123 72 2 17 135 78 199 2.05e-17 86.7
Msa0422250 AT3G46640.2 51.899 79 37 1 190 267 135 213 6.66e-17 82.4
Msa0422250 AT3G46640.1 51.899 79 37 1 190 267 135 213 6.66e-17 82.4
Msa0422250 AT3G46640.3 51.899 79 37 1 190 267 135 213 1.07e-16 82.0
Msa0422250 AT2G40970.1 57.377 61 26 0 198 258 104 164 4.63e-16 78.6
Msa0422250 AT3G10760.1 57.377 61 26 0 198 258 104 164 7.17e-16 79.7
Msa0422250 AT5G05090.1 55.738 61 27 0 198 258 80 140 2.24e-15 77.0
Msa0422250 AT5G44190.2 47.368 76 40 0 198 273 146 221 2.52e-14 75.1
Msa0422250 AT5G44190.1 47.368 76 40 0 198 273 146 221 2.73e-14 75.5
Msa0422250 AT2G20570.1 36.667 120 71 2 158 272 107 226 3.12e-13 72.4
Msa0422250 AT4G18020.8 34.074 135 80 3 12 139 13 145 6.96e-13 71.6
Msa0422250 AT4G18020.8 37.383 107 56 2 156 253 246 350 1.91e-12 70.1
Msa0422250 AT4G18020.11 34.074 135 80 3 12 139 13 145 6.96e-13 71.6
Msa0422250 AT4G18020.11 37.383 107 56 2 156 253 246 350 1.91e-12 70.1
Msa0422250 AT4G18020.4 34.074 135 80 3 12 139 13 145 6.96e-13 71.6
Msa0422250 AT4G18020.4 37.383 107 56 2 156 253 246 350 1.91e-12 70.1
Msa0422250 AT4G18020.5 34.074 135 80 3 12 139 13 145 6.96e-13 71.6
Msa0422250 AT4G18020.5 37.383 107 56 2 156 253 246 350 1.91e-12 70.1
Msa0422250 AT4G18020.10 34.074 135 80 3 12 139 13 145 6.96e-13 71.6
Msa0422250 AT4G18020.10 37.383 107 56 2 156 253 246 350 1.91e-12 70.1
Msa0422250 AT4G18020.7 34.074 135 80 3 12 139 13 145 6.96e-13 71.6
Msa0422250 AT4G18020.7 37.383 107 56 2 156 253 246 350 1.91e-12 70.1
Msa0422250 AT4G18020.3 35.115 131 76 3 12 135 13 141 9.79e-13 71.2
Msa0422250 AT4G18020.3 37.383 107 56 2 156 253 246 350 1.67e-12 70.5
Msa0422250 AT4G18020.2 35.115 131 76 3 12 135 13 141 9.79e-13 71.2
Msa0422250 AT4G18020.2 37.383 107 56 2 156 253 246 350 1.67e-12 70.5
Msa0422250 AT4G18020.1 35.115 131 76 3 12 135 13 141 9.79e-13 71.2
Msa0422250 AT4G18020.1 37.383 107 56 2 156 253 246 350 1.67e-12 70.5
Msa0422250 AT4G18020.6 35.115 131 76 3 12 135 13 141 9.79e-13 71.2
Msa0422250 AT4G18020.6 37.383 107 56 2 156 253 246 350 1.67e-12 70.5
Msa0422250 AT4G18020.9 35.115 131 76 3 12 135 13 141 9.79e-13 71.2
Msa0422250 AT4G18020.9 37.383 107 56 2 156 253 246 350 1.67e-12 70.5
Msa0422250 AT3G57040.2 27.778 162 91 4 19 158 11 168 1.57e-12 67.8
Msa0422250 AT3G57040.1 27.778 162 91 4 19 158 11 168 1.57e-12 67.8
Msa0422250 AT2G20570.2 44.444 72 40 0 201 272 171 242 4.21e-12 68.9
Msa0422250 AT1G69580.1 42.857 84 47 1 186 268 21 104 2.06e-11 65.9
Msa0422250 AT1G69580.2 42.857 84 47 1 186 268 21 104 2.19e-11 65.9

Find 121 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATGCTGCTTGCCATTTA+AGG 0.123652 3_2:+71363816 None:intergenic
TTCTTTGTTGCCTTATAATA+AGG 0.193391 3_2:+71366825 None:intergenic
CGCAACTCTTTCATCCTTAT+AGG 0.193820 3_2:+71365364 None:intergenic
ACACGACGACAAAGAATTTC+TGG 0.206213 3_2:-71365164 Msa0422250:CDS
CTCCCAAAAGTAGGTTTCTT+TGG 0.223208 3_2:+71363512 None:intergenic
TTACTCATTGCCTTATTATA+AGG 0.223385 3_2:-71366835 Msa0422250:five_prime_UTR
TTCAACCTATTGCCCTTAAA+TGG 0.231156 3_2:-71363829 Msa0422250:CDS
ATCATTCATCATAAGGCTTC+CGG 0.251176 3_2:+71363621 None:intergenic
CCTATTTCCACTACTAATTT+CGG 0.260366 3_2:-71363586 Msa0422250:CDS
GGCAACATGTCTTCAGAAAA+AGG 0.266185 3_2:-71365333 Msa0422250:CDS
GGAATTTCAGTCCAAGAATA+TGG 0.271706 3_2:+71363904 None:intergenic
CTGAATAAGCAGGTAATATC+AGG 0.277996 3_2:+71363302 None:intergenic
TCAAAAGATGTAGGAAATTT+CGG 0.297315 3_2:-71364192 Msa0422250:CDS
TATTGAACAATTATAAAGAT+TGG 0.304100 3_2:-71363212 Msa0422250:three_prime_UTR
TCTGATTCGAGTGGGTTAAA+AGG 0.305989 3_2:+71363964 None:intergenic
CTTTCATCCTTATAGGCTTA+AGG 0.308983 3_2:+71365371 None:intergenic
AGATCCAAATTCTTGATTAT+AGG 0.309304 3_2:+71363177 None:intergenic
TTACCAAAGAAACCTACTTT+TGG 0.311221 3_2:-71363515 Msa0422250:CDS
GAATGTTGCTAGCCACTTAC+AGG 0.318285 3_2:-71364603 Msa0422250:intron
CCGAAATTAGTAGTGGAAAT+AGG 0.318852 3_2:+71363586 None:intergenic
ACTATCTCCTTAAGCCTATA+AGG 0.322260 3_2:-71365378 Msa0422250:CDS
TGAAGCAGCTTCTGCGCCTT+TGG 0.331281 3_2:-71363488 Msa0422250:CDS
AGTATATATCCCTTTGTGTT+TGG 0.334763 3_2:+71366951 None:intergenic
ACGAGGACTTATATTTCTAT+TGG 0.343534 3_2:-71363465 Msa0422250:CDS
ATTGAACAATTATAAAGATT+GGG 0.346199 3_2:-71363211 Msa0422250:three_prime_UTR
ACATGTCAACACCATATTCT+TGG 0.360381 3_2:-71363915 Msa0422250:CDS
TTATGCTGCTTGCCATTTAA+GGG 0.367070 3_2:+71363817 None:intergenic
GGAATGTGCAACAATTCCTT+TGG 0.372703 3_2:+71364056 None:intergenic
GTTGAATCATATAAGTGGTT+TGG 0.380619 3_2:+71363370 None:intergenic
GCTTGCCATTTAAGGGCAAT+AGG 0.380909 3_2:+71363824 None:intergenic
TCATTCATCATAAGGCTTCC+GGG 0.383196 3_2:+71363622 None:intergenic
CAAAGAAAGCTCGAGTAGTT+TGG 0.384580 3_2:-71365129 Msa0422250:CDS
ATATCCGATCCTTTATACTT+TGG 0.391843 3_2:+71363027 None:intergenic
TGAAAGAGTTGCGAAATATA+TGG 0.396476 3_2:-71365354 Msa0422250:CDS
AACTTTGGTCAACAGAATAT+AGG 0.396927 3_2:-71363433 Msa0422250:CDS
TAGGTTGATCATTCATCATA+AGG 0.400146 3_2:+71363614 None:intergenic
GCGGGTTAACCAAAGTATAA+AGG 0.410704 3_2:-71363036 None:intergenic
TATTGGCATAATATGAACTT+TGG 0.411212 3_2:-71363448 Msa0422250:CDS
ACTTTGGTCAACAGAATATA+GGG 0.416874 3_2:-71363432 Msa0422250:CDS
TGTTGCTTGTTTGCTGAGTT+CGG 0.418693 3_2:+71364165 None:intergenic
GAATGTGCAACAATTCCTTT+GGG 0.430333 3_2:+71364057 None:intergenic
AGCATGCACTTGATGAGAAA+TGG 0.443162 3_2:-71365268 Msa0422250:CDS
GATTTACCTTCAAAAGATGT+AGG 0.444043 3_2:-71364201 Msa0422250:CDS
GACATCTTGCTCTGTTATAG+AGG 0.450734 3_2:+71363747 None:intergenic
GTTAAAGCAGTGAATCAGAT+TGG 0.453872 3_2:-71365082 Msa0422250:CDS
CTCAAGAAGTGTAATTATGA+AGG 0.461617 3_2:-71366649 Msa0422250:intron
AACTACTCGAGCTTTCTTTG+AGG 0.464350 3_2:+71365132 None:intergenic
AGCTTCTGCGCCTTTGGACG+AGG 0.465782 3_2:-71363482 Msa0422250:CDS
TTGAACAATTATAAAGATTG+GGG 0.468210 3_2:-71363210 Msa0422250:three_prime_UTR
CTTGCAGCATGTATAAAAGT+AGG 0.474079 3_2:-71363156 Msa0422250:three_prime_UTR
TCATATCTTCTAAAGCAAAC+AGG 0.481173 3_2:+71365221 None:intergenic
AGTTTGAAACCGTCCATGTC+GGG 0.482203 3_2:+71365620 None:intergenic
AATTTGTTGTGCAGAAATAC+CGG 0.482831 3_2:-71364290 Msa0422250:intron
TCAAGGATCTTAAGCCATGT+TGG 0.493168 3_2:+71366679 None:intergenic
AATCTGTTGAATCATATAAG+TGG 0.495988 3_2:+71363365 None:intergenic
GGAACTTCTATGAGAAGTCC+TGG 0.496302 3_2:+71363391 None:intergenic
CTGCTTCACCTCCCAAAAGT+AGG 0.498434 3_2:+71363503 None:intergenic
TGTTCAATAATAGATGTAGT+TGG 0.504586 3_2:+71363226 None:intergenic
GATTATTCAGTAATTGATCA+AGG 0.504935 3_2:-71363346 Msa0422250:CDS
ATGTCTGAGTATTATGATCC+AGG 0.508275 3_2:-71363409 Msa0422250:CDS
TGTGCATCAACGAATATTAG+TGG 0.517100 3_2:+71366897 None:intergenic
CTGGAACAAAATAAATAAGA+AGG 0.518397 3_2:+71363093 None:intergenic
TTCAAACTTCTTGAGCATGT+TGG 0.518961 3_2:-71365605 Msa0422250:CDS
TTGCAGCATGTATAAAAGTA+GGG 0.519598 3_2:-71363155 Msa0422250:three_prime_UTR
CTCTAAGCTTGCTTCGTGAA+AGG 0.524950 3_2:-71365682 Msa0422250:CDS
AAAATATGAATTGTAACTGC+GGG 0.524959 3_2:-71363054 Msa0422250:three_prime_UTR
CAAGAAAGACTACACTAGTT+CGG 0.525368 3_2:-71363686 Msa0422250:CDS
TGAGCATGTTGGACTTGAGA+TGG 0.531729 3_2:-71365594 Msa0422250:CDS
CCGTGGATGGAGAAACAAGC+AGG 0.533449 3_2:-71365432 Msa0422250:CDS
ATAGTAACATCACCTGTAAG+TGG 0.535698 3_2:+71364591 None:intergenic
AGAATGCTTCATTCCACTTG+AGG 0.537639 3_2:+71364224 None:intergenic
GAACTGAAACAGAAGCAGCT+TGG 0.544464 3_2:-71366787 Msa0422250:five_prime_UTR
CCTGCTTGTTTCTCCATCCA+CGG 0.545386 3_2:+71365432 None:intergenic
TCATCATAAGGCTTCCGGGT+TGG 0.545654 3_2:+71363626 None:intergenic
GAAGTCTACTCAAGTAAAGC+CGG 0.548598 3_2:+71364271 None:intergenic
GACAAATACAACTATTCTGA+AGG 0.548895 3_2:-71364126 Msa0422250:CDS
ATAAGGCTTCCGGGTTGGAA+AGG 0.569730 3_2:+71363631 None:intergenic
GTAAACATGCCCGACATGGA+CGG 0.570099 3_2:-71365629 Msa0422250:CDS
TTGCTCTGTTATAGAGGGAG+CGG 0.570348 3_2:+71363753 None:intergenic
GACACCTTTCCTTTCCAACC+CGG 0.571228 3_2:-71363640 Msa0422250:CDS
GTAGTGGAAATAGGTTGTGT+AGG 0.571324 3_2:+71363595 None:intergenic
AGGACTAGACCAAACACAAA+GGG 0.576648 3_2:-71366960 Msa0422250:five_prime_UTR
ATAGAAACTAAAAGTCATGA+AGG 0.577004 3_2:-71364084 Msa0422250:CDS
CGATGTAAACATGCCCGACA+TGG 0.580139 3_2:-71365633 Msa0422250:CDS
GGATCAGAGATGTCTGAGGA+GGG 0.581360 3_2:-71365247 Msa0422250:CDS
ATAGAGAACTGACTAATGAC+AGG 0.581856 3_2:+71365566 None:intergenic
AAATATAAGTCCTCGTCCAA+AGG 0.586082 3_2:+71363472 None:intergenic
CTTGTATAGAAACAAAACCT+AGG 0.593384 3_2:-71363720 Msa0422250:CDS
ACAAGAACTCGTAAACCAGC+AGG 0.595339 3_2:+71366715 None:intergenic
TTCTTGTAATCATAAAACCT+AGG 0.596152 3_2:+71363703 None:intergenic
TAGATTTGATGAATGTTCCA+TGG 0.598681 3_2:-71364637 Msa0422250:CDS
TATTAGTGGTGGATAAGTAA+TGG 0.602951 3_2:+71366911 None:intergenic
ACATCTTGCTCTGTTATAGA+GGG 0.608912 3_2:+71363748 None:intergenic
ACATTCTCTCTACTCAGCCA+TGG 0.609214 3_2:+71364620 None:intergenic
TTGCTTCGTGAAAGGAAAGA+CGG 0.611540 3_2:-71365674 Msa0422250:CDS
AAGTTTGAAACCGTCCATGT+CGG 0.611862 3_2:+71365619 None:intergenic
TGGATCAGAGATGTCTGAGG+AGG 0.612409 3_2:-71365248 Msa0422250:CDS
TGAACAATTATAAAGATTGG+GGG 0.614567 3_2:-71363209 Msa0422250:three_prime_UTR
AAAGTCACTTGTTCATCTAG+AGG 0.616120 3_2:-71363857 Msa0422250:CDS
GATAGTCACATGCACCGTGT+TGG 0.616316 3_2:+71365395 None:intergenic
GATCAGAGATGTCTGAGGAG+GGG 0.621652 3_2:-71365246 Msa0422250:CDS
GAAACAAGCAGGGTGATGAA+AGG 0.627020 3_2:-71365421 Msa0422250:CDS
CTCGAATCAGAACGAAAACG+TGG 0.629850 3_2:-71363952 Msa0422250:CDS
TGCAGCATGTATAAAAGTAG+GGG 0.629866 3_2:-71363154 Msa0422250:three_prime_UTR
AGTCATGAAGGTAATCCCAA+AGG 0.634878 3_2:-71364072 Msa0422250:CDS
TATATACTAACATACGCCAC+AGG 0.646847 3_2:-71366936 Msa0422250:five_prime_UTR
TGAATAAGCAGGTAATATCA+GGG 0.650140 3_2:+71363303 None:intergenic
GCATCAACGAATATTAGTGG+TGG 0.650656 3_2:+71366900 None:intergenic
TGGTTTACGAGTTCTTGTTG+TGG 0.652536 3_2:-71366710 Msa0422250:CDS
CGTGGATGGAGAAACAAGCA+GGG 0.655838 3_2:-71365431 Msa0422250:CDS
TGTTCAGTGATGTCCGTGGA+TGG 0.656358 3_2:-71365445 Msa0422250:intron
TAGGACTAGACCAAACACAA+AGG 0.665199 3_2:-71366961 Msa0422250:five_prime_UTR
AAATGGATCAGAGATGTCTG+AGG 0.666125 3_2:-71365251 Msa0422250:CDS
GTGATGAAAGGTGTCCAACA+CGG 0.667153 3_2:-71365409 Msa0422250:CDS
TGGATAAGTAATGGTTCCTG+TGG 0.678696 3_2:+71366920 None:intergenic
ATGTCTTGCTAAGCAACATG+TGG 0.682204 3_2:+71365706 None:intergenic
CCAGCAGGAAAATCGCGTCG+AGG 0.689105 3_2:+71366730 None:intergenic
GCTTCCGGGTTGGAAAGGAA+AGG 0.689286 3_2:+71363636 None:intergenic
GCAGCATGTATAAAAGTAGG+GGG 0.692190 3_2:-71363153 Msa0422250:three_prime_UTR
ATGCTGTTCAGTGATGTCCG+TGG 0.714764 3_2:-71365449 Msa0422250:intron
TTGTGGATGATGATCCAACA+TGG 0.719364 3_2:-71366693 Msa0422250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCTAAATTTATTGTTTTT+TGG - chr3_2:71363929-71363948 Msa0422250:CDS 10.0%
!!! TGTTATTTGTTTCATAATTT+TGG - chr3_2:71363520-71363539 Msa0422250:CDS 15.0%
!!! TTATATGCTTATTTGAATTT+TGG + chr3_2:71363620-71363639 None:intergenic 15.0%
!! AATTATAAACTATTTCCATA+TGG - chr3_2:71365210-71365229 Msa0422250:CDS 15.0%
!! TATTGAACAATTATAAAGAT+TGG - chr3_2:71366789-71366808 Msa0422250:five_prime_UTR 15.0%
!! ATTGAACAATTATAAAGATT+GGG - chr3_2:71366790-71366809 Msa0422250:five_prime_UTR 15.0%
!!! CAAAAAAATTTTGAAAGGTA+AGG + chr3_2:71363728-71363747 None:intergenic 20.0%
!!! TGCTACAAAAAAATTTTGAA+AGG + chr3_2:71363733-71363752 None:intergenic 20.0%
!! GAAAAACTTGAATTATTAGA+TGG - chr3_2:71363794-71363813 Msa0422250:CDS 20.0%
!!! TGCTATTTTTAAGACATAAA+AGG - chr3_2:71363869-71363888 Msa0422250:CDS 20.0%
!!! GCTATTTTTAAGACATAAAA+GGG - chr3_2:71363870-71363889 Msa0422250:CDS 20.0%
!!! TATTTTTCAGTCAAAATTGT+AGG - chr3_2:71364125-71364144 Msa0422250:CDS 20.0%
!! ATGAAAAAAGTAACAGAAAA+AGG + chr3_2:71364159-71364178 None:intergenic 20.0%
!!! CTTTTAATCAAATTGATCAA+AGG + chr3_2:71364504-71364523 None:intergenic 20.0%
!!! ATCAAATCTAGTATTTTCTT+GGG + chr3_2:71365354-71365373 None:intergenic 20.0%
!! TTGAACAATTATAAAGATTG+GGG - chr3_2:71366791-71366810 Msa0422250:five_prime_UTR 20.0%
!!! TTTTCCTATAATCAAGAATT+TGG - chr3_2:71366820-71366839 Msa0422250:five_prime_UTR 20.0%
!! AAAAATATGAATTGTAACTG+CGG - chr3_2:71366946-71366965 Msa0422250:five_prime_UTR 20.0%
!! TTACTCATTGCCTTATTATA+AGG - chr3_2:71363166-71363185 Msa0422250:three_prime_UTR 25.0%
! TTCTTTGTTGCCTTATAATA+AGG + chr3_2:71363179-71363198 None:intergenic 25.0%
!! GTTTCAGTTCAAAAAAGTTT+TGG + chr3_2:71363204-71363223 None:intergenic 25.0%
! TAATTAGCACATAACAAAAG+AGG + chr3_2:71363402-71363421 None:intergenic 25.0%
! GTTTCTGAATTTAAATGTGT+TGG - chr3_2:71363548-71363567 Msa0422250:CDS 25.0%
! TTGGATTATAAAATGTCTCT+TGG - chr3_2:71363567-71363586 Msa0422250:CDS 25.0%
!! AAGTTTTTCTTCTTGAAGAT+CGG + chr3_2:71363783-71363802 None:intergenic 25.0%
!!! GAGGGTTATAAACAATTTTT+TGG - chr3_2:71363822-71363841 Msa0422250:CDS 25.0%
!! AGATTAGCTAGAATCAAATT+CGG + chr3_2:71363914-71363933 None:intergenic 25.0%
!!! TTGTTTTTTGGCATTGTTTA+TGG - chr3_2:71363941-71363960 Msa0422250:CDS 25.0%
! AAAAGTAACAGAAAAAGGAT+TGG + chr3_2:71364154-71364173 None:intergenic 25.0%
!! GTCACAGAATTAACAAATTT+TGG - chr3_2:71364215-71364234 Msa0422250:CDS 25.0%
! TGATAGTAAGTTTCTTGTTA+AGG - chr3_2:71364945-71364964 Msa0422250:intron 25.0%
!! AGGTTCTCTAAATTGTTATT+CGG - chr3_2:71364965-71364984 Msa0422250:intron 25.0%
!!! AGCTATTTCTTTAATCAAAG+AGG - chr3_2:71365115-71365134 Msa0422250:CDS 25.0%
!!! GCTATTTCTTTAATCAAAGA+GGG - chr3_2:71365116-71365135 Msa0422250:CDS 25.0%
! CTTAATTCAATAAACTCTTC+GGG + chr3_2:71365171-71365190 None:intergenic 25.0%
! GCTTAATTCAATAAACTCTT+CGG + chr3_2:71365172-71365191 None:intergenic 25.0%
! ATTAAGCTGAAAACAACTAA+AGG - chr3_2:71365185-71365204 Msa0422250:CDS 25.0%
!! CATCAAATCTAGTATTTTCT+TGG + chr3_2:71365355-71365374 None:intergenic 25.0%
!! ACTTCTTGAATATTTTCCTT+AGG - chr3_2:71365436-71365455 Msa0422250:CDS 25.0%
! CAAAAACAAGAGAAGTTAAA+AGG - chr3_2:71365626-71365645 Msa0422250:CDS 25.0%
!!! CACAACAAATTTTTTATCGT+TGG + chr3_2:71365702-71365721 None:intergenic 25.0%
!! AAATTTCCTACATCTTTTGA+AGG + chr3_2:71365809-71365828 None:intergenic 25.0%
! TCAAAAGATGTAGGAAATTT+CGG - chr3_2:71365809-71365828 Msa0422250:intron 25.0%
! ATAGAAACTAAAAGTCATGA+AGG - chr3_2:71365917-71365936 Msa0422250:intron 25.0%
! TTCTTGTAATCATAAAACCT+AGG + chr3_2:71366301-71366320 None:intergenic 25.0%
! TATTGGCATAATATGAACTT+TGG - chr3_2:71366553-71366572 Msa0422250:intron 25.0%
! AATCTGTTGAATCATATAAG+TGG + chr3_2:71366639-71366658 None:intergenic 25.0%
! GATTATTCAGTAATTGATCA+AGG - chr3_2:71366655-71366674 Msa0422250:CDS 25.0%
!!! ATTTTTTTTGCTGAATAAGC+AGG + chr3_2:71366712-71366731 None:intergenic 25.0%
! TGTTCAATAATAGATGTAGT+TGG + chr3_2:71366778-71366797 None:intergenic 25.0%
! TGAACAATTATAAAGATTGG+GGG - chr3_2:71366792-71366811 Msa0422250:five_prime_UTR 25.0%
! AGATCCAAATTCTTGATTAT+AGG + chr3_2:71366827-71366846 None:intergenic 25.0%
! CTGGAACAAAATAAATAAGA+AGG + chr3_2:71366911-71366930 None:intergenic 25.0%
!!! CTTCTTATTTATTTTGTTCC+AGG - chr3_2:71366909-71366928 Msa0422250:five_prime_UTR 25.0%
! AAAATATGAATTGTAACTGC+GGG - chr3_2:71366947-71366966 Msa0422250:five_prime_UTR 25.0%
AGTATATATCCCTTTGTGTT+TGG + chr3_2:71363053-71363072 None:intergenic 30.0%
!! TATTAGTGGTGGATAAGTAA+TGG + chr3_2:71363093-71363112 None:intergenic 30.0%
! CTCAAGAAGTGTAATTATGA+AGG - chr3_2:71363352-71363371 Msa0422250:CDS 30.0%
GTTGATGTACTATACTACTA+AGG - chr3_2:71363440-71363459 Msa0422250:CDS 30.0%
CTACTAAGGATCTTCAAAAT+TGG - chr3_2:71363454-71363473 Msa0422250:CDS 30.0%
TGTCTTAAAAATAGCAGACA+TGG + chr3_2:71363865-71363884 None:intergenic 30.0%
TAAGACATAAAAGGGAACAA+AGG - chr3_2:71363878-71363897 Msa0422250:CDS 30.0%
TGAAAGAGTTGCGAAATATA+TGG - chr3_2:71364647-71364666 Msa0422250:CDS 30.0%
!! TCATATCTTCTAAAGCAAAC+AGG + chr3_2:71364783-71364802 None:intergenic 30.0%
! ATCTTTTCTTTTCTTCGTTG+AGG + chr3_2:71364806-71364825 None:intergenic 30.0%
TTATGCTTGACAATCACAAT+TGG + chr3_2:71365240-71365259 None:intergenic 30.0%
TGTAACAAGTGAATTCTGTT+TGG - chr3_2:71365317-71365336 Msa0422250:CDS 30.0%
TAGATTTGATGAATGTTCCA+TGG - chr3_2:71365364-71365383 Msa0422250:CDS 30.0%
!! GTTTCTTTCTAGAATTCCTA+AGG + chr3_2:71365455-71365474 None:intergenic 30.0%
!! AATTTGTTGTGCAGAAATAC+CGG - chr3_2:71365711-71365730 Msa0422250:CDS 30.0%
GATTTACCTTCAAAAGATGT+AGG - chr3_2:71365800-71365819 Msa0422250:intron 30.0%
GACAAATACAACTATTCTGA+AGG - chr3_2:71365875-71365894 Msa0422250:intron 30.0%
!!! TCTTTTTTGAGCTGTGTTTT+TGG + chr3_2:71366121-71366140 None:intergenic 30.0%
CTTGTATAGAAACAAAACCT+AGG - chr3_2:71366281-71366300 Msa0422250:intron 30.0%
!! TAGGTTGATCATTCATCATA+AGG + chr3_2:71366390-71366409 None:intergenic 30.0%
CCTATTTCCACTACTAATTT+CGG - chr3_2:71366415-71366434 Msa0422250:intron 30.0%
! TTTTAAGCCGAAATTAGTAG+TGG + chr3_2:71366425-71366444 None:intergenic 30.0%
! TTACCAAAGAAACCTACTTT+TGG - chr3_2:71366486-71366505 Msa0422250:intron 30.0%
!! TACCAAAGAAACCTACTTTT+GGG - chr3_2:71366487-71366506 Msa0422250:intron 30.0%
! ACGAGGACTTATATTTCTAT+TGG - chr3_2:71366536-71366555 Msa0422250:intron 30.0%
AACTTTGGTCAACAGAATAT+AGG - chr3_2:71366568-71366587 Msa0422250:intron 30.0%
ACTTTGGTCAACAGAATATA+GGG - chr3_2:71366569-71366588 Msa0422250:intron 30.0%
GTTGAATCATATAAGTGGTT+TGG + chr3_2:71366634-71366653 None:intergenic 30.0%
TGAATAAGCAGGTAATATCA+GGG + chr3_2:71366701-71366720 None:intergenic 30.0%
TTGCAGCATGTATAAAAGTA+GGG - chr3_2:71366846-71366865 Msa0422250:five_prime_UTR 30.0%
!!! TTTTTTTTTCAAGCAAGTCC+TGG + chr3_2:71366930-71366949 None:intergenic 30.0%
TATATACTAACATACGCCAC+AGG - chr3_2:71363065-71363084 Msa0422250:three_prime_UTR 35.0%
TGTGCATCAACGAATATTAG+TGG + chr3_2:71363107-71363126 None:intergenic 35.0%
GGTCTCTCAAAAATGGATAA+TGG - chr3_2:71363235-71363254 Msa0422250:three_prime_UTR 35.0%
! ACTTCTTGAGCATCTTTTCA+AGG + chr3_2:71363342-71363361 None:intergenic 35.0%
TTTCTTCTTGAAGATCGGAA+AGG + chr3_2:71363778-71363797 None:intergenic 35.0%
CTTGAATTATTAGATGGTGC+AGG - chr3_2:71363800-71363819 Msa0422250:CDS 35.0%
AATTATTAGATGGTGCAGGA+GGG - chr3_2:71363804-71363823 Msa0422250:CDS 35.0%
GGCATTGTTTATGGTATCTT+TGG - chr3_2:71363950-71363969 Msa0422250:CDS 35.0%
! CTTTGGAAATAACATGCCAA+TGG - chr3_2:71363967-71363986 Msa0422250:CDS 35.0%
ATGTTAGTGCATGTTACCAA+TGG + chr3_2:71364000-71364019 None:intergenic 35.0%
TTCAAACTTCTTGAGCATGT+TGG - chr3_2:71364396-71364415 Msa0422250:intron 35.0%
ATAGAGAACTGACTAATGAC+AGG + chr3_2:71364438-71364457 None:intergenic 35.0%
ACTATCTCCTTAAGCCTATA+AGG - chr3_2:71364623-71364642 Msa0422250:CDS 35.0%
CTTTCATCCTTATAGGCTTA+AGG + chr3_2:71364633-71364652 None:intergenic 35.0%
GTTAAAGCAGTGAATCAGAT+TGG - chr3_2:71364919-71364938 Msa0422250:intron 35.0%
ATAGTAACATCACCTGTAAG+TGG + chr3_2:71365413-71365432 None:intergenic 35.0%
GAACAAAAATCTTCCTCAAG+TGG - chr3_2:71365764-71365783 Msa0422250:intron 35.0%
GAATGTGCAACAATTCCTTT+GGG + chr3_2:71365947-71365966 None:intergenic 35.0%
! GAAAAAGAAAGAGCTTTGAC+TGG - chr3_2:71365977-71365996 Msa0422250:intron 35.0%
ACATGTCAACACCATATTCT+TGG - chr3_2:71366086-71366105 Msa0422250:intron 35.0%
GGAATTTCAGTCCAAGAATA+TGG + chr3_2:71366100-71366119 None:intergenic 35.0%
AAAGTCACTTGTTCATCTAG+AGG - chr3_2:71366144-71366163 Msa0422250:intron 35.0%
TTCAACCTATTGCCCTTAAA+TGG - chr3_2:71366172-71366191 Msa0422250:intron 35.0%
! TTATGCTGCTTGCCATTTAA+GGG + chr3_2:71366187-71366206 None:intergenic 35.0%
ACATCTTGCTCTGTTATAGA+GGG + chr3_2:71366256-71366275 None:intergenic 35.0%
CAAGAAAGACTACACTAGTT+CGG - chr3_2:71366315-71366334 Msa0422250:intron 35.0%
ATCATTCATCATAAGGCTTC+CGG + chr3_2:71366383-71366402 None:intergenic 35.0%
! CCGAAATTAGTAGTGGAAAT+AGG + chr3_2:71366418-71366437 None:intergenic 35.0%
AAATATAAGTCCTCGTCCAA+AGG + chr3_2:71366532-71366551 None:intergenic 35.0%
ATGTCTGAGTATTATGATCC+AGG - chr3_2:71366592-71366611 Msa0422250:intron 35.0%
CTGAATAAGCAGGTAATATC+AGG + chr3_2:71366702-71366721 None:intergenic 35.0%
CTTGCAGCATGTATAAAAGT+AGG - chr3_2:71366845-71366864 Msa0422250:five_prime_UTR 35.0%
TGCAGCATGTATAAAAGTAG+GGG - chr3_2:71366847-71366866 Msa0422250:five_prime_UTR 35.0%
TAGGACTAGACCAAACACAA+AGG - chr3_2:71363040-71363059 Msa0422250:three_prime_UTR 40.0%
AGGACTAGACCAAACACAAA+GGG - chr3_2:71363041-71363060 Msa0422250:three_prime_UTR 40.0%
TGGATAAGTAATGGTTCCTG+TGG + chr3_2:71363084-71363103 None:intergenic 40.0%
GCATCAACGAATATTAGTGG+TGG + chr3_2:71363104-71363123 None:intergenic 40.0%
TGGTTTACGAGTTCTTGTTG+TGG - chr3_2:71363291-71363310 Msa0422250:three_prime_UTR 40.0%
TTGTGGATGATGATCCAACA+TGG - chr3_2:71363308-71363327 Msa0422250:three_prime_UTR 40.0%
TCAAGGATCTTAAGCCATGT+TGG + chr3_2:71363325-71363344 None:intergenic 40.0%
GAATTATTAGATGGTGCAGG+AGG - chr3_2:71363803-71363822 Msa0422250:CDS 40.0%
TGGAAATAACATGCCAATGG+TGG - chr3_2:71363970-71363989 Msa0422250:CDS 40.0%
!! TACCAATGGATTACCACCAT+TGG + chr3_2:71363986-71364005 None:intergenic 40.0%
ATGTCTTGCTAAGCAACATG+TGG + chr3_2:71364298-71364317 None:intergenic 40.0%
TTGCTTCGTGAAAGGAAAGA+CGG - chr3_2:71364327-71364346 Msa0422250:intron 40.0%
! AAGTTTGAAACCGTCCATGT+CGG + chr3_2:71364385-71364404 None:intergenic 40.0%
CGCAACTCTTTCATCCTTAT+AGG + chr3_2:71364640-71364659 None:intergenic 40.0%
GGCAACATGTCTTCAGAAAA+AGG - chr3_2:71364668-71364687 Msa0422250:intron 40.0%
CTTCAGAAAAAGGATACACG+AGG - chr3_2:71364678-71364697 Msa0422250:intron 40.0%
AGCATGCACTTGATGAGAAA+TGG - chr3_2:71364733-71364752 Msa0422250:intron 40.0%
AAATGGATCAGAGATGTCTG+AGG - chr3_2:71364750-71364769 Msa0422250:intron 40.0%
ACACGACGACAAAGAATTTC+TGG - chr3_2:71364837-71364856 Msa0422250:intron 40.0%
AACTACTCGAGCTTTCTTTG+AGG + chr3_2:71364872-71364891 None:intergenic 40.0%
CAAAGAAAGCTCGAGTAGTT+TGG - chr3_2:71364872-71364891 Msa0422250:intron 40.0%
CTTTAATCAAAGAGGGAGTG+TGG - chr3_2:71365123-71365142 Msa0422250:CDS 40.0%
ATCACAATTGGAAGGCCATA+TGG + chr3_2:71365228-71365247 None:intergenic 40.0%
GCTTGACAATCACAATTGGA+AGG + chr3_2:71365236-71365255 None:intergenic 40.0%
GAATTCTGTTTGGCAGAAGT+TGG - chr3_2:71365327-71365346 Msa0422250:CDS 40.0%
GAAGTCTACTCAAGTAAAGC+CGG + chr3_2:71365733-71365752 None:intergenic 40.0%
AGAATGCTTCATTCCACTTG+AGG + chr3_2:71365780-71365799 None:intergenic 40.0%
! TGTTGCTTGTTTGCTGAGTT+CGG + chr3_2:71365839-71365858 None:intergenic 40.0%
AGTCATGAAGGTAATCCCAA+AGG - chr3_2:71365929-71365948 Msa0422250:intron 40.0%
GGAATGTGCAACAATTCCTT+TGG + chr3_2:71365948-71365967 None:intergenic 40.0%
! TCTTTCTTTTTCTGCAGCAG+TGG + chr3_2:71365969-71365988 None:intergenic 40.0%
!! TCTGATTCGAGTGGGTTAAA+AGG + chr3_2:71366040-71366059 None:intergenic 40.0%
!! GTTTTCGTTCTGATTCGAGT+GGG + chr3_2:71366048-71366067 None:intergenic 40.0%
! GTTATGCTGCTTGCCATTTA+AGG + chr3_2:71366188-71366207 None:intergenic 40.0%
GACATCTTGCTCTGTTATAG+AGG + chr3_2:71366257-71366276 None:intergenic 40.0%
TCATTCATCATAAGGCTTCC+GGG + chr3_2:71366382-71366401 None:intergenic 40.0%
! GTAGTGGAAATAGGTTGTGT+AGG + chr3_2:71366409-71366428 None:intergenic 40.0%
CTCCCAAAAGTAGGTTTCTT+TGG + chr3_2:71366492-71366511 None:intergenic 40.0%
!! CAAAGAAACCTACTTTTGGG+AGG - chr3_2:71366490-71366509 Msa0422250:intron 40.0%
GCAGCATGTATAAAAGTAGG+GGG - chr3_2:71366848-71366867 Msa0422250:five_prime_UTR 40.0%
GAACTGAAACAGAAGCAGCT+TGG - chr3_2:71363214-71363233 Msa0422250:three_prime_UTR 45.0%
GCAGCTTGGTCTCTCAAAAA+TGG - chr3_2:71363228-71363247 Msa0422250:three_prime_UTR 45.0%
ACAAGAACTCGTAAACCAGC+AGG + chr3_2:71363289-71363308 None:intergenic 45.0%
GCTAGAATCAAATTCGGCGA+GGG + chr3_2:71363908-71363927 None:intergenic 45.0%
AGCTAGAATCAAATTCGGCG+AGG + chr3_2:71363909-71363928 None:intergenic 45.0%
TGCCAATGGTGGTAATCCAT+TGG - chr3_2:71363981-71364000 Msa0422250:CDS 45.0%
TATAGTTGAGACCCTAGCAG+TGG - chr3_2:71364031-71364050 Msa0422250:CDS 45.0%
AGTCTTAGATGCCACTGCTA+GGG + chr3_2:71364045-71364064 None:intergenic 45.0%
! CTCTAAGCTTGCTTCGTGAA+AGG - chr3_2:71364319-71364338 Msa0422250:intron 45.0%
! AGTTTGAAACCGTCCATGTC+GGG + chr3_2:71364384-71364403 None:intergenic 45.0%
TGAGCATGTTGGACTTGAGA+TGG - chr3_2:71364407-71364426 Msa0422250:intron 45.0%
GAAACAAGCAGGGTGATGAA+AGG - chr3_2:71364580-71364599 Msa0422250:intron 45.0%
!! GTGATGAAAGGTGTCCAACA+CGG - chr3_2:71364592-71364611 Msa0422250:intron 45.0%
CACTCGTTTAGCAATGTGCT+AGG + chr3_2:71365033-71365052 None:intergenic 45.0%
ACATTCTCTCTACTCAGCCA+TGG + chr3_2:71365384-71365403 None:intergenic 45.0%
GAATGTTGCTAGCCACTTAC+AGG - chr3_2:71365398-71365417 Msa0422250:CDS 45.0%
!! CGTTTTCGTTCTGATTCGAG+TGG + chr3_2:71366049-71366068 None:intergenic 45.0%
CTCGAATCAGAACGAAAACG+TGG - chr3_2:71366049-71366068 Msa0422250:intron 45.0%
! GCTTGCCATTTAAGGGCAAT+AGG + chr3_2:71366180-71366199 None:intergenic 45.0%
TTGCTCTGTTATAGAGGGAG+CGG + chr3_2:71366251-71366270 None:intergenic 45.0%
GGAACTTCTATGAGAAGTCC+TGG + chr3_2:71366613-71366632 None:intergenic 45.0%
GAGTCTTAGATGCCACTGCT+AGG + chr3_2:71364046-71364065 None:intergenic 50.0%
! AGCAGTGGCATCTAAGACTC+AGG - chr3_2:71364046-71364065 Msa0422250:CDS 50.0%
! GCAGTGGCATCTAAGACTCA+GGG - chr3_2:71364047-71364066 Msa0422250:CDS 50.0%
CGATGTAAACATGCCCGACA+TGG - chr3_2:71364368-71364387 Msa0422250:intron 50.0%
GTAAACATGCCCGACATGGA+CGG - chr3_2:71364372-71364391 Msa0422250:intron 50.0%
ATGCTGTTCAGTGATGTCCG+TGG - chr3_2:71364552-71364571 Msa0422250:intron 50.0%
TGTTCAGTGATGTCCGTGGA+TGG - chr3_2:71364556-71364575 Msa0422250:intron 50.0%
! CCTGCTTGTTTCTCCATCCA+CGG + chr3_2:71364572-71364591 None:intergenic 50.0%
CGTGGATGGAGAAACAAGCA+GGG - chr3_2:71364570-71364589 Msa0422250:intron 50.0%
GATAGTCACATGCACCGTGT+TGG + chr3_2:71364609-71364628 None:intergenic 50.0%
TGGATCAGAGATGTCTGAGG+AGG - chr3_2:71364753-71364772 Msa0422250:intron 50.0%
GGATCAGAGATGTCTGAGGA+GGG - chr3_2:71364754-71364773 Msa0422250:intron 50.0%
GATCAGAGATGTCTGAGGAG+GGG - chr3_2:71364755-71364774 Msa0422250:intron 50.0%
GACACCTTTCCTTTCCAACC+CGG - chr3_2:71366361-71366380 Msa0422250:intron 50.0%
! ATAAGGCTTCCGGGTTGGAA+AGG + chr3_2:71366373-71366392 None:intergenic 50.0%
TCATCATAAGGCTTCCGGGT+TGG + chr3_2:71366378-71366397 None:intergenic 50.0%
CTGCTTCACCTCCCAAAAGT+AGG + chr3_2:71366501-71366520 None:intergenic 50.0%
CCGTGGATGGAGAAACAAGC+AGG - chr3_2:71364569-71364588 Msa0422250:intron 55.0%
! GCTTCCGGGTTGGAAAGGAA+AGG + chr3_2:71366368-71366387 None:intergenic 55.0%
TGAAGCAGCTTCTGCGCCTT+TGG - chr3_2:71366513-71366532 Msa0422250:intron 55.0%
CCAGCAGGAAAATCGCGTCG+AGG + chr3_2:71363274-71363293 None:intergenic 60.0%
! CCTCGACGCGATTTTCCTGC+TGG - chr3_2:71363271-71363290 Msa0422250:three_prime_UTR 60.0%
AGCTTCTGCGCCTTTGGACG+AGG - chr3_2:71366519-71366538 Msa0422250:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 71363039 71366984 71363039 ID=Msa0422250;Name=Msa0422250
chr3_2 mRNA 71363039 71366984 71363039 ID=Msa0422250-mRNA-1;Parent=Msa0422250;Name=Msa0422250-mRNA-1;_AED=0.05;_eAED=0.05;_QI=208|1|1|1|1|1|5|291|570
chr3_2 exon 71363039 71364298 71363039 ID=Msa0422250-mRNA-1:exon:17442;Parent=Msa0422250-mRNA-1
chr3_2 exon 71364604 71364680 71364604 ID=Msa0422250-mRNA-1:exon:17441;Parent=Msa0422250-mRNA-1
chr3_2 exon 71365074 71365460 71365074 ID=Msa0422250-mRNA-1:exon:17440;Parent=Msa0422250-mRNA-1
chr3_2 exon 71365579 71365731 71365579 ID=Msa0422250-mRNA-1:exon:17439;Parent=Msa0422250-mRNA-1
chr3_2 exon 71366650 71366984 71366650 ID=Msa0422250-mRNA-1:exon:17438;Parent=Msa0422250-mRNA-1
chr3_2 five_prime_UTR 71366777 71366984 71366777 ID=Msa0422250-mRNA-1:five_prime_utr;Parent=Msa0422250-mRNA-1
chr3_2 CDS 71366650 71366776 71366650 ID=Msa0422250-mRNA-1:cds;Parent=Msa0422250-mRNA-1
chr3_2 CDS 71365579 71365731 71365579 ID=Msa0422250-mRNA-1:cds;Parent=Msa0422250-mRNA-1
chr3_2 CDS 71365074 71365460 71365074 ID=Msa0422250-mRNA-1:cds;Parent=Msa0422250-mRNA-1
chr3_2 CDS 71364604 71364680 71364604 ID=Msa0422250-mRNA-1:cds;Parent=Msa0422250-mRNA-1
chr3_2 CDS 71363330 71364298 71363330 ID=Msa0422250-mRNA-1:cds;Parent=Msa0422250-mRNA-1
chr3_2 three_prime_UTR 71363039 71363329 71363039 ID=Msa0422250-mRNA-1:three_prime_utr;Parent=Msa0422250-mRNA-1
Gene Sequence

>Msa0422250

ATGGATAATGGTTGCTTCTCTTCGCCTCGACGCGATTTTCCTGCTGGTTTACGAGTTCTTGTTGTGGATGATGATCCAACATGGCTTAAGATCCTTGAAAAGATGCTCAAGAAGTGTAATTATGAAGTGACCACATGTTGCTTAGCAAGACATGCTCTAAGCTTGCTTCGTGAAAGGAAAGACGGATACGATATAGTGATCAGCGATGTAAACATGCCCGACATGGACGGTTTCAAACTTCTTGAGCATGTTGGACTTGAGATGGATCTTCCTGTCATTATGATGTCCGTGGATGGAGAAACAAGCAGGGTGATGAAAGGTGTCCAACACGGTGCATGTGACTATCTCCTTAAGCCTATAAGGATGAAAGAGTTGCGAAATATATGGCAACATGTCTTCAGAAAAAGGATACACGAGGTTAGAGAATTTGAAAATTTTGAGAGTTTTGAGAGCATGCACTTGATGAGAAATGGATCAGAGATGTCTGAGGAGGGGAACCTGTTTGCTTTAGAAGATATGACCTCAACGAAGAAAAGAAAAGATGTAGATAGCAGACACGACGACAAAGAATTTCTGGATTCATCTTCCTCAAAGAAAGCTCGAGTAGTTTGGTCTGTAGATCTTCATCAGAAGTTTGTTAAAGCAGTGAATCAGATTGGATTTGATAAAGTTGGTCCCAAGAAAATACTAGATTTGATGAATGTTCCATGGCTGAGTAGAGAGAATGTTGCTAGCCACTTACAGAAATACCGGCTTTACTTGAGTAGACTTCAGAAAGATAACGAACAAAAATCTTCCTCAAGTGGAATGAAGCATTCTGATTTACCTTCAAAAGATGTAGGAAATTTCGGTGTTCCGAACTCAGCAAACAAGCAACAAAATGATGCTTCAATCGACAAATACAACTATTCTGAAGGAAGCTTACAACATCAGAACATAGAAACTAAAAGTCATGAAGGTAATCCCAAAGGAATTGTTGCACATTCCACTGCTGCAGAAAAAGAAAGAGCTTTGACTGGTAACATCACTGATGCAACAGACATTGCACTTAATAAACCTTTTAACCCACTCGAATCAGAACGAAAACGTGGAGCGTTTGATTGCAACATGTCAACACCATATTCTTGGACTGAAATTCCAAAAACACAGCTCAAAAAAGAACAAAAGTCACTTGTTCATCTAGAGGATAACTTCAACCTATTGCCCTTAAATGGCAAGCAGCATAACATTCAAGTTGATCAATCACAGTCAATTGCTTCAATTAGTTCCGCTCCCTCTATAACAGAGCAAGATGTCGCTGCTTGTATAGAAACAAAACCTAGGTTTTATGATTACAAGAAAGACTACACTAGTTCGGTGAGTTCAAGAAGAACTTCAGTTGACACCTTTCCTTTCCAACCCGGAAGCCTTATGATGAATGATCAACCTACACAACCTATTTCCACTACTAATTTCGGCTTAAAAACACAGACATCTAATCTTAGTTGCATTTCTGATCTAGAGTCTTACCAAAGAAACCTACTTTTGGGAGGTGAAGCAGCTTCTGCGCCTTTGGACGAGGACTTATATTTCTATTGGCATAATATGAACTTTGGTCAACAGAATATAGGGATGTCTGAGTATTATGATCCAGGACTTCTCATAGAAGTTCCAAACCACTTATATGATTCAACAGATTATTCAGTAATTGATCAAGGTCTATTCATAGCATGA
Protein sequence

>Msa0422250

MDNGCFSSPRRDFPAGLRVLVVDDDPTWLKILEKMLKKCNYEVTTCCLARHALSLLRERKDGYDIVISDVNMPDMDGFKLLEHVGLEMDLPVIMMSVDGETSRVMKGVQHGACDYLLKPIRMKELRNIWQHVFRKRIHEVREFENFESFESMHLMRNGSEMSEEGNLFALEDMTSTKKRKDVDSRHDDKEFLDSSSSKKARVVWSVDLHQKFVKAVNQIGFDKVGPKKILDLMNVPWLSRENVASHLQKYRLYLSRLQKDNEQKSSSSGMKHSDLPSKDVGNFGVPNSANKQQNDASIDKYNYSEGSLQHQNIETKSHEGNPKGIVAHSTAAEKERALTGNITDATDIALNKPFNPLESERKRGAFDCNMSTPYSWTEIPKTQLKKEQKSLVHLEDNFNLLPLNGKQHNIQVDQSQSIASISSAPSITEQDVAACIETKPRFYDYKKDYTSSVSSRRTSVDTFPFQPGSLMMNDQPTQPISTTNFGLKTQTSNLSCISDLESYQRNLLLGGEAASAPLDEDLYFYWHNMNFGQQNIGMSEYYDPGLLIEVPNHLYDSTDYSVIDQGLFIA*