AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0429710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0429710 MtrunA17_Chr3g0133481 68.182 396 119 1 162 557 1 389 0.0 542
Msa0429710 MtrunA17_Chr3g0133481 64.539 141 43 1 1 141 1 134 1.58e-49 176
Msa0429710 MtrunA17_Chr3g0133511 62.716 405 133 5 162 555 1 398 1.38e-172 494
Msa0429710 MtrunA17_Chr3g0133511 57.143 140 53 2 1 140 1 133 1.08e-39 149
Msa0429710 MtrunA17_Chr3g0133391 63.750 400 130 5 162 557 1 389 1.38e-167 481
Msa0429710 MtrunA17_Chr3g0133391 44.221 199 91 5 1 199 1 179 2.71e-39 147
Msa0429710 MtrunA17_Chr7g0233731 60.349 401 147 3 158 553 7 400 2.98e-165 477
Msa0429710 MtrunA17_Chr7g0233731 52.143 140 60 1 2 141 12 144 3.40e-38 146
Msa0429710 MtrunA17_Chr7g0233731 70.968 62 17 1 497 557 399 460 9.04e-17 83.2
Msa0429710 MtrunA17_Chr8g0346621 63.077 390 127 6 173 557 20 397 1.84e-164 473
Msa0429710 MtrunA17_Chr8g0346621 50.532 188 77 4 12 199 20 191 2.01e-41 154
Msa0429710 MtrunA17_Chr4g0023671 61.347 401 137 5 162 557 1 388 1.05e-163 471
Msa0429710 MtrunA17_Chr4g0023671 53.571 140 56 2 1 140 1 131 7.24e-37 141
Msa0429710 MtrunA17_Chr3g0083861 56.984 451 172 7 111 555 5 439 8.93e-163 470
Msa0429710 MtrunA17_Chr3g0083861 59.124 137 47 2 4 140 56 183 2.80e-38 146
Msa0429710 MtrunA17_Chr7g0233701 62.500 400 138 3 163 557 4 396 1.02e-160 463
Msa0429710 MtrunA17_Chr7g0233701 45.312 192 88 3 2 193 4 178 1.28e-39 149
Msa0429710 MtrunA17_Chr3g0133411 54.955 444 160 8 144 557 5 438 4.15e-157 456
Msa0429710 MtrunA17_Chr3g0133411 60.169 118 47 0 22 139 44 161 7.50e-38 145
Msa0429710 MtrunA17_Chr3g0133431 59.453 402 141 9 162 556 20 406 3.04e-147 429
Msa0429710 MtrunA17_Chr3g0133431 38.000 200 99 7 1 198 20 196 8.61e-25 106
Msa0429710 MtrunA17_Chr3g0133531 69.128 298 91 1 259 555 7 304 1.33e-142 414
Msa0429710 MtrunA17_Chr3g0133531 70.455 44 13 0 98 141 7 50 1.90e-11 65.5
Msa0429710 MtrunA17_Chr3g0096411 54.412 408 144 7 166 556 9 391 1.83e-140 412
Msa0429710 MtrunA17_Chr3g0096411 48.630 146 63 2 5 140 9 152 1.14e-33 132
Msa0429710 MtrunA17_Chr2g0302581 54.887 399 157 6 165 555 3 386 2.21e-139 409
Msa0429710 MtrunA17_Chr2g0302581 51.471 136 54 3 4 139 3 126 6.46e-28 115
Msa0429710 MtrunA17_Chr2g0302611 54.887 399 157 6 165 555 3 386 3.19e-139 408
Msa0429710 MtrunA17_Chr2g0302611 51.471 136 54 3 4 139 3 126 2.56e-28 116
Msa0429710 MtrunA17_Chr3g0134681 56.283 382 128 6 183 556 67 417 9.94e-136 400
Msa0429710 MtrunA17_Chr3g0134681 58.333 120 50 0 22 141 67 186 4.00e-39 148
Msa0429710 MtrunA17_Chr3g0083001 55.062 405 133 8 158 555 9 371 9.64e-132 389
Msa0429710 MtrunA17_Chr3g0083001 54.745 137 53 2 4 140 14 141 1.80e-38 145
Msa0429710 MtrunA17_Chr7g0233691 67.286 269 83 2 294 557 10 278 7.19e-121 357
Msa0429710 MtrunA17_Chr8g0358841 66.541 266 84 2 295 555 2 267 6.10e-120 354
Msa0429710 MtrunA17_Chr7g0233721 67.939 262 79 2 301 557 17 278 9.02e-118 349
Msa0429710 MtrunA17_Chr3g0133371 66.797 256 81 2 304 557 35 288 1.33e-117 349
Msa0429710 MtrunA17_Chr4g0046911 62.069 261 88 3 301 556 5 259 1.50e-104 315
Msa0429710 MtrunA17_Chr7g0233751 54.693 309 115 4 166 467 7 297 1.21e-103 314
Msa0429710 MtrunA17_Chr7g0233751 43.367 196 106 2 5 199 7 198 1.06e-39 146
Msa0429710 MtrunA17_Chr3g0083041 63.035 257 88 5 303 555 44 297 3.99e-101 307
Msa0429710 MtrunA17_Chr3g0083871 56.705 261 88 3 300 555 9 249 2.28e-91 280
Msa0429710 MtrunA17_Chr8g0346661 66.667 213 69 1 183 395 15 225 5.63e-91 278
Msa0429710 MtrunA17_Chr8g0346661 65.546 119 39 1 22 140 15 131 1.00e-41 149
Msa0429710 MtrunA17_Chr3g0141031 61.860 215 74 2 348 557 9 220 2.70e-80 251
Msa0429710 MtrunA17_Chr3g0143151 39.899 396 208 12 181 557 26 410 1.49e-73 240
Msa0429710 MtrunA17_Chr3g0143151 35.714 126 69 3 20 139 26 145 5.25e-12 68.2
Msa0429710 MtrunA17_Chr2g0302701 57.488 207 77 3 357 555 9 212 1.68e-71 228
Msa0429710 MtrunA17_Chr3g0133091 62.644 174 59 2 341 508 1 174 5.42e-68 218
Msa0429710 MtrunA17_Chr3g0132731 68.243 148 47 0 298 445 11 158 8.45e-66 211
Msa0429710 MtrunA17_Chr3g0133501 67.089 158 45 2 406 556 1 158 1.43e-61 200
Msa0429710 MtrunA17_Chr3g0133401 57.065 184 43 2 374 557 1 148 6.96e-60 195
Msa0429710 MtrunA17_Chr3g0133471 62.275 167 52 4 374 531 1 165 3.68e-57 189
Msa0429710 MtrunA17_Chr8g0346651 59.615 156 54 3 406 557 1 151 1.77e-52 176
Msa0429710 MtrunA17_Chr3g0134651 56.129 155 64 1 365 515 3 157 2.17e-51 174
Msa0429710 MtrunA17_Chr4g0046951 66.667 114 38 0 301 414 5 118 4.99e-49 166
Msa0429710 MtrunA17_Chr7g0233711 55.319 141 56 1 163 303 4 137 3.72e-44 154
Msa0429710 MtrunA17_Chr7g0233711 55.797 138 54 1 2 139 4 134 1.68e-43 152
Msa0429710 MtrunA17_Chr7g0233681 52.414 145 59 2 160 301 4 141 2.28e-42 148
Msa0429710 MtrunA17_Chr7g0233681 53.191 141 65 1 1 140 1 141 7.51e-42 147
Msa0429710 MtrunA17_Chr3g0133351 55.333 150 57 3 1 150 1 140 3.48e-41 146
Msa0429710 MtrunA17_Chr3g0133351 58.696 138 47 3 162 299 1 128 6.11e-41 145
Msa0429710 MtrunA17_Chr3g0134661 65.385 104 36 0 184 287 1 104 4.75e-40 140
Msa0429710 MtrunA17_Chr3g0134661 66.667 102 34 0 23 124 1 102 1.06e-39 140
Msa0429710 MtrunA17_Chr8g0358851 56.522 138 53 1 162 299 1 131 7.00e-40 141
Msa0429710 MtrunA17_Chr8g0358851 55.396 139 55 1 1 139 1 132 9.29e-39 138
Msa0429710 MtrunA17_Chr3g0133521 64.583 96 34 0 301 396 2 97 7.56e-38 134
Msa0429710 MtrunA17_Chr4g0046941 56.452 124 52 1 22 145 16 137 1.43e-36 132
Msa0429710 MtrunA17_Chr4g0046941 58.120 117 47 1 183 299 16 130 1.44e-35 130
Msa0429710 MtrunA17_Chr4g0046961 57.851 121 42 2 435 551 2 117 6.97e-31 117
Msa0429710 MtrunA17_Chr3g0141021 53.521 71 33 0 284 354 1 71 1.02e-21 89.7
Msa0429710 MtrunA17_Chr3g0132691 67.213 61 20 0 374 434 1 61 1.50e-21 88.6
Msa0429710 MtrunA17_Chr3g0133491 89.130 46 5 0 512 557 1 46 2.17e-21 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0429710 AT1G47710.1 48.500 400 190 7 162 555 1 390 1.13e-121 364
Msa0429710 AT1G47710.1 44.286 140 69 4 1 140 1 131 1.01e-23 103
Msa0429710 AT1G47710.2 48.379 401 191 7 161 555 27 417 1.77e-121 364
Msa0429710 AT1G47710.2 44.286 140 69 4 1 140 28 158 1.63e-23 103
Msa0429710 AT3G45220.1 45.924 368 179 4 200 554 30 390 2.82e-107 327
Msa0429710 AT3G45220.1 39.604 101 61 0 39 139 30 130 3.28e-14 75.1
Msa0429710 AT2G25240.1 47.658 363 172 7 200 554 30 382 4.14e-106 324
Msa0429710 AT2G25240.1 39.604 101 57 1 39 139 30 126 2.19e-11 66.2
Msa0429710 AT2G26390.1 46.594 367 173 8 200 554 31 386 9.41e-105 320
Msa0429710 AT2G26390.1 40.594 101 56 1 39 139 31 127 7.92e-15 77.0
Msa0429710 AT1G64030.1 42.386 394 198 6 165 554 3 371 8.93e-97 300
Msa0429710 AT1G64030.1 34.507 142 84 3 4 144 3 136 3.22e-17 84.3
Msa0429710 AT1G62170.1 42.935 368 180 5 197 556 88 433 9.68e-96 298
Msa0429710 AT1G62170.1 36.283 113 68 1 36 144 88 200 2.08e-13 72.8
Msa0429710 AT2G14540.1 44.875 361 178 4 197 554 55 397 1.07e-93 292
Msa0429710 AT2G14540.1 30.201 149 90 4 36 177 55 196 1.00e-13 73.6
Msa0429710 AT1G62170.2 39.651 401 179 6 197 556 88 466 8.05e-90 284
Msa0429710 AT1G62170.2 36.937 111 66 1 36 142 88 198 3.32e-14 75.5
Msa0429710 AT2G35580.1 42.577 357 181 7 204 554 33 371 1.61e-89 280
Msa0429710 AT2G35580.1 30.263 152 89 4 43 192 33 169 1.47e-14 75.9
Msa0429710 AT1G64010.2 47.907 215 91 3 341 554 1 195 5.28e-62 203
Msa0429710 AT1G64010.1 47.000 200 85 3 356 554 2 181 3.42e-54 182
Msa0429710 AT1G62160.1 41.000 200 91 5 363 556 42 220 9.83e-34 128
Msa0429710 AT1G63280.1 53.390 118 51 2 297 412 5 120 1.27e-33 124
Msa0429710 AT1G51330.1 49.107 112 54 2 298 406 29 140 7.06e-27 108
Msa0429710 AT1G64020.1 35.398 113 70 3 179 290 10 120 2.11e-12 64.7
Msa0429710 AT1G64020.1 37.500 104 62 3 18 120 10 111 2.28e-12 64.7

Find 126 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCCAATCAGTGAATTTA+TGG 0.159817 3_2:-81185113 Msa0429710:CDS
TATGAGGAAGCTTGTCTTCT+AGG 0.205127 3_2:+81184734 None:intergenic
AACTGTTGCTATTCATTATA+TGG 0.237017 3_2:-81186081 Msa0429710:CDS
CTTGTCTTTATATTCTTCTT+TGG 0.244807 3_2:+81185737 None:intergenic
CTTGTCTTTATATTCTTCTT+TGG 0.244807 3_2:+81186352 None:intergenic
TGTCTTCTGCCAACGCTGTT+TGG 0.258315 3_2:-81185527 Msa0429710:CDS
TGTCTTCTGCCAACGCTGTT+TGG 0.258315 3_2:-81186142 Msa0429710:CDS
GTCTTCTGCCAACGCTGTTT+GGG 0.287560 3_2:-81185526 Msa0429710:CDS
GTCTTCTGCCAACGCTGTTT+GGG 0.287560 3_2:-81186141 Msa0429710:CDS
CCTCTTCATGCTAGTCATAA+AGG 0.293948 3_2:+81184909 None:intergenic
AACTGTTGCTACTCATTATA+TGG 0.315904 3_2:-81185466 Msa0429710:CDS
GATGCAAAAGATGGGTTGTT+AGG 0.331478 3_2:-81184790 Msa0429710:CDS
CGGAAGCGACGTGAGAAGAT+AGG 0.331599 3_2:+81185589 None:intergenic
CGGAAGCGACGTGAGAAGAT+AGG 0.331599 3_2:+81186204 None:intergenic
GCTGCCGCATTCACTGGAAT+TGG 0.345550 3_2:-81184514 Msa0429710:CDS
TCATCTTCACCTTGCTTGTA+AGG 0.348281 3_2:+81184844 None:intergenic
TGCTAAGAAACGAGATATAT+TGG 0.359665 3_2:+81185893 None:intergenic
AATCAGCTTCTGTCCTTTCT+CGG 0.370054 3_2:-81185639 Msa0429710:CDS
AATCAGCTTCTGTCCTTTCT+CGG 0.370054 3_2:-81186254 Msa0429710:CDS
CGAGGAGACTGAGCGCAGTT+TGG 0.373483 3_2:+81185688 None:intergenic
CGAGGAGACTGAGCGCAGTT+TGG 0.373483 3_2:+81186303 None:intergenic
TCTGGTACAATACTCTTTGT+TGG 0.375689 3_2:-81184400 Msa0429710:CDS
TCTGATGTGTTGAAGGAGTT+AGG 0.381460 3_2:-81184649 Msa0429710:CDS
AGCCCATAAATTCACTGATT+GGG 0.386856 3_2:+81185110 None:intergenic
AATAGCAACAGTTTCTTTGA+AGG 0.390569 3_2:+81186091 None:intergenic
ATTCTTCTGGTTTGAATGGT+TGG 0.391216 3_2:+81185849 None:intergenic
AATACTGTAGAAACACTAAT+TGG 0.391329 3_2:+81184577 None:intergenic
CAGCCCATAAATTCACTGAT+TGG 0.401585 3_2:+81185109 None:intergenic
GGAGCAATCGTTTGATGCTT+CGG 0.404813 3_2:-81184983 Msa0429710:CDS
CTGGTACAATACTCTTTGTT+GGG 0.410353 3_2:-81184399 Msa0429710:CDS
TGAAATTTCTGATGTGTTGA+AGG 0.418942 3_2:-81184656 Msa0429710:CDS
AACCATTCAAACCAGAAGAA+TGG 0.422288 3_2:-81185847 Msa0429710:CDS
ATTCTTCTTTGGAGAACAAA+TGG 0.423124 3_2:+81185748 None:intergenic
ATTCTTCTTTGGAGAACAAA+TGG 0.423124 3_2:+81186363 None:intergenic
ATCCATTCTTCTGGTTTGAA+TGG 0.424273 3_2:+81185845 None:intergenic
CTCTTCATGCTAGTCATAAA+GGG 0.432869 3_2:+81184910 None:intergenic
TTTCTTCCAGATGCAAAAGA+TGG 0.444703 3_2:-81184799 Msa0429710:CDS
AGTAGCAACAGTTTCTTTGA+AGG 0.447416 3_2:+81185476 None:intergenic
TGGTGAAGAAATGGTTGGTA+AGG 0.449170 3_2:+81185799 None:intergenic
TGGTGAAGAAATGGTTGGTA+AGG 0.449170 3_2:+81186414 None:intergenic
CTGCCGCATTCACTGGAATT+GGG 0.449429 3_2:-81184513 Msa0429710:CDS
AGTGAATTTATGGGCTGAAA+AGG 0.450449 3_2:-81185103 Msa0429710:CDS
TTCACTGGAATTGGGATGGC+TGG 0.452119 3_2:-81184505 Msa0429710:CDS
TTGATTAGAGAAGATTTGTC+TGG 0.453212 3_2:-81184418 Msa0429710:CDS
ACGAACGTAATCCATTCTTC+TGG 0.456785 3_2:+81185836 None:intergenic
AACCCAATCAGTGAATTTAT+GGG 0.457167 3_2:-81185112 Msa0429710:CDS
GGAAGCGACGTGAGAAGATA+GGG 0.462599 3_2:+81185590 None:intergenic
GGAAGCGACGTGAGAAGATA+GGG 0.462599 3_2:+81186205 None:intergenic
CTCCTCGCTGTTGGTTCTGA+GGG 0.470422 3_2:-81185672 Msa0429710:CDS
CTCCTCGCTGTTGGTTCTGA+GGG 0.470422 3_2:-81186287 Msa0429710:CDS
TCAAAAGAAATATTGAATCT+TGG 0.470594 3_2:+81184682 None:intergenic
TGAAGGAAGGGTAAACAGAA+AGG 0.471470 3_2:+81185493 None:intergenic
TGAAGGAAGGGTAAACAGAA+AGG 0.471470 3_2:+81186108 None:intergenic
TGGGCTGAAAAGGAGACAAA+AGG 0.472940 3_2:-81185093 Msa0429710:CDS
ACTAAAGCTGCCGCATTCAC+TGG 0.473621 3_2:-81184520 Msa0429710:CDS
GACAAGCATGGACCGCCGGC+AGG 0.474957 3_2:+81185546 None:intergenic
GACAAGCATGGACCGCCGGC+AGG 0.474957 3_2:+81186161 None:intergenic
GTCAAACCTCCAAAAGAGAA+AGG 0.475976 3_2:+81184616 None:intergenic
CAACAGTTTCTTTGAAGGAA+GGG 0.487406 3_2:+81186096 None:intergenic
TCTCCTCGCTGTTGGTTCTG+AGG 0.490976 3_2:-81185673 Msa0429710:CDS
TCTCCTCGCTGTTGGTTCTG+AGG 0.490976 3_2:-81186288 Msa0429710:CDS
GGAATCTCAGTTTCAATGCT+CGG 0.492213 3_2:+81185870 None:intergenic
GAAGGAAGGGTAAACAGAAA+GGG 0.492254 3_2:+81185494 None:intergenic
GAAGGAAGGGTAAACAGAAA+GGG 0.492254 3_2:+81186109 None:intergenic
CAACAGTTTCTTTGAAGGAA+GGG 0.497373 3_2:+81185481 None:intergenic
CTCAAAGACGCTGCTCCTGC+CGG 0.500555 3_2:-81185561 Msa0429710:CDS
CTCAAAGACGCTGCTCCTGC+CGG 0.500555 3_2:-81186176 Msa0429710:CDS
GCAACAGTTTCTTTGAAGGA+AGG 0.503967 3_2:+81186095 None:intergenic
TTCTTTGGAGAACAAATGGT+TGG 0.505452 3_2:+81185752 None:intergenic
TTCTTTGGAGAACAAATGGT+TGG 0.505452 3_2:+81186367 None:intergenic
GCGCTCAGTCTCCTCGCTGT+TGG 0.506186 3_2:-81185681 Msa0429710:CDS
GCGCTCAGTCTCCTCGCTGT+TGG 0.506186 3_2:-81186296 Msa0429710:CDS
GGTATCGGAGTTGAAGTGTA+TGG 0.511402 3_2:+81184475 None:intergenic
GCAACAGTTTCTTTGAAGGA+AGG 0.513025 3_2:+81185480 None:intergenic
TGGTCAGCTACAAAGTCTAT+TGG 0.513464 3_2:+81184454 None:intergenic
ACAAATGCATTATACTTCAA+AGG 0.517977 3_2:-81185012 Msa0429710:CDS
GTCATTGAAGTAAATGAAGA+AGG 0.521214 3_2:-81184544 Msa0429710:CDS
TTCTTCCAGATGCAAAAGAT+GGG 0.522138 3_2:-81184798 Msa0429710:CDS
CGCATTCACTGGAATTGGGA+TGG 0.526890 3_2:-81184509 Msa0429710:CDS
GGGCTGAAAAGGAGACAAAA+GGG 0.540477 3_2:-81185092 Msa0429710:CDS
AGAAGACAAGCATGGACCGC+CGG 0.545801 3_2:+81185542 None:intergenic
AGAAGACAAGCATGGACCGC+CGG 0.545801 3_2:+81186157 None:intergenic
AGCGCAGTTTGGAGCGACAA+TGG 0.549289 3_2:+81185699 None:intergenic
AGCGCAGTTTGGAGCGACAA+TGG 0.549289 3_2:+81186314 None:intergenic
CAAGTGCATAATCCCCTCGC+TGG 0.556254 3_2:-81184376 Msa0429710:CDS
GCTACAAAGTCTATTGGTAT+CGG 0.556429 3_2:+81184460 None:intergenic
CATTATACTTCAAAGGAGCA+TGG 0.562007 3_2:-81185005 Msa0429710:CDS
CTTAGTCTTCCTTACAAGCA+AGG 0.563134 3_2:-81184853 Msa0429710:CDS
GTTAGGTTTGATTGAGAAAG+CGG 0.567281 3_2:-81184773 Msa0429710:CDS
GGAGAACAAATGGTTGGCGA+TGG 0.571262 3_2:+81185758 None:intergenic
GGAGAACAAATGGTTGGCGA+TGG 0.571262 3_2:+81186373 None:intergenic
CCTTTATGACTAGCATGAAG+AGG 0.571406 3_2:-81184909 Msa0429710:CDS
GATGGACTTTGATCCGAGAA+AGG 0.581696 3_2:+81185626 None:intergenic
GATGGACTTTGATCCGAGAA+AGG 0.581696 3_2:+81186241 None:intergenic
CAAGCTTCCTCATACAAAAG+TGG 0.592026 3_2:-81184725 Msa0429710:CDS
TCTCAATGGCACCTCAGTCA+AGG 0.599345 3_2:-81184935 Msa0429710:CDS
ATTATACTTCAAAGGAGCAT+GGG 0.601138 3_2:-81185004 Msa0429710:CDS
ATCACTTGATTTCACTAACA+AGG 0.601544 3_2:-81186048 Msa0429710:intron
TAGGGAGTTGAGATGGCCGA+TGG 0.601672 3_2:+81185608 None:intergenic
TAGGGAGTTGAGATGGCCGA+TGG 0.601672 3_2:+81186223 None:intergenic
CTTGCTTGTAAGGAAGACTA+AGG 0.608424 3_2:+81184854 None:intergenic
GAGAAGATAGGGAGTTGAGA+TGG 0.618975 3_2:+81185601 None:intergenic
GAGAAGATAGGGAGTTGAGA+TGG 0.618975 3_2:+81186216 None:intergenic
AAAGACGCTGCTCCTGCCGG+CGG 0.624527 3_2:-81185558 Msa0429710:CDS
AAAGACGCTGCTCCTGCCGG+CGG 0.624527 3_2:-81186173 Msa0429710:CDS
GAAATCAAGTGATTTGAGAG+TGG 0.625530 3_2:+81185443 None:intergenic
CCTCATACAAAAGTGGTGGT+AGG 0.629641 3_2:-81184718 Msa0429710:CDS
CATCCCAATTCCAGTGAATG+CGG 0.632365 3_2:+81184510 None:intergenic
GAAATCAAGTGATTTGAGAG+TGG 0.634588 3_2:+81186058 None:intergenic
TGTGTTGAAGGAGTTAGGAG+TGG 0.642269 3_2:-81184644 Msa0429710:CDS
TAGTTGAGATCATCCAGCGA+GGG 0.653122 3_2:+81184363 None:intergenic
TTTCTCGGATCAAAGTCCAT+CGG 0.660434 3_2:-81185624 Msa0429710:CDS
TTTCTCGGATCAAAGTCCAT+CGG 0.660434 3_2:-81186239 Msa0429710:CDS
GTTAAGCTATTAATCGACCC+AGG 0.661094 3_2:+81185048 None:intergenic
AGCAGCGTCTTTGAGAACAG+CGG 0.665031 3_2:+81185569 None:intergenic
AGCAGCGTCTTTGAGAACAG+CGG 0.665031 3_2:+81186184 None:intergenic
TGACTGAGGTGCCATTGAGA+AGG 0.673594 3_2:+81184938 None:intergenic
GCTTCCTCATACAAAAGTGG+TGG 0.680566 3_2:-81184722 Msa0429710:CDS
AGTTGAGATCATCCAGCGAG+GGG 0.687329 3_2:+81184364 None:intergenic
GCGTTGGCAGAAGACAAGCA+TGG 0.713189 3_2:+81185534 None:intergenic
GCGTTGGCAGAAGACAAGCA+TGG 0.713189 3_2:+81186149 None:intergenic
CATAAAGGGAACCTTGACTG+AGG 0.721556 3_2:+81184924 None:intergenic
GACCCTCAGAACCAACAGCG+AGG 0.731995 3_2:+81185670 None:intergenic
GACCCTCAGAACCAACAGCG+AGG 0.731995 3_2:+81186285 None:intergenic
AAGCTATTAATCGACCCAGG+TGG 0.746620 3_2:+81185051 None:intergenic
GTAGTTGAGATCATCCAGCG+AGG 0.762097 3_2:+81184362 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAGTTTTTTTTTTTTTAG+AGG - chr3_2:81184804-81184823 Msa0429710:CDS 10.0%
!!! TTAGTTTTTTTTTTTTTAGA+GGG - chr3_2:81184805-81184824 Msa0429710:CDS 10.0%
!!! ATAAATTGCAATTTTTTTTT+GGG + chr3_2:81184879-81184898 None:intergenic 10.0%
!!! AATAAATTGCAATTTTTTTT+TGG + chr3_2:81184880-81184899 None:intergenic 10.0%
!!! ATTAGTTTTTTTTTTTTTAG+AGG - chr3_2:81184804-81184823 Msa0429710:CDS 10.0%
!!! TTAGTTTTTTTTTTTTTAGA+GGG - chr3_2:81184805-81184824 Msa0429710:CDS 10.0%
!!! ATAAATTGCAATTTTTTTTT+GGG + chr3_2:81184879-81184898 None:intergenic 10.0%
!!! AATAAATTGCAATTTTTTTT+TGG + chr3_2:81184880-81184899 None:intergenic 10.0%
!!! ATTGTTTAGAATTGAAAATA+AGG + chr3_2:81185483-81185502 None:intergenic 15.0%
!!! TATATTTGATATTGTTTTGA+AGG - chr3_2:81185670-81185689 Msa0429710:CDS 15.0%
!!! ATTGTTTAGAATTGAAAATA+AGG + chr3_2:81185483-81185502 None:intergenic 15.0%
!!! TATATTTGATATTGTTTTGA+AGG - chr3_2:81185670-81185689 Msa0429710:CDS 15.0%
!! GAAAAGTTGACATATTTATA+GGG - chr3_2:81184836-81184855 Msa0429710:CDS 20.0%
!!! TTTTGGGTTAAATATGTTTT+TGG + chr3_2:81184863-81184882 None:intergenic 20.0%
!! TTAATCTACTAATCTAACAA+GGG + chr3_2:81185454-81185473 None:intergenic 20.0%
!! TTTAATCTACTAATCTAACA+AGG + chr3_2:81185455-81185474 None:intergenic 20.0%
!!! TATTTTGTTGTTAAATTGCT+TGG + chr3_2:81185561-81185580 None:intergenic 20.0%
!! TCAAAAGAAATATTGAATCT+TGG + chr3_2:81186136-81186155 None:intergenic 20.0%
!! GAAAAGTTGACATATTTATA+GGG - chr3_2:81184836-81184855 Msa0429710:CDS 20.0%
!!! TTTTGGGTTAAATATGTTTT+TGG + chr3_2:81184863-81184882 None:intergenic 20.0%
!! TTAATCTACTAATCTAACAA+GGG + chr3_2:81185454-81185473 None:intergenic 20.0%
!! TTTAATCTACTAATCTAACA+AGG + chr3_2:81185455-81185474 None:intergenic 20.0%
!!! TATTTTGTTGTTAAATTGCT+TGG + chr3_2:81185561-81185580 None:intergenic 20.0%
!! TCAAAAGAAATATTGAATCT+TGG + chr3_2:81186136-81186155 None:intergenic 20.0%
!!! TTTCTTTTTTGGTGAAGAAA+TGG + chr3_2:81184413-81184432 None:intergenic 25.0%
!! GGTCATAGATTTTTCTTTTT+TGG + chr3_2:81184424-81184443 None:intergenic 25.0%
! CTTGTCTTTATATTCTTCTT+TGG + chr3_2:81184466-81184485 None:intergenic 25.0%
!! AACTGTTGCTATTCATTATA+TGG - chr3_2:81184734-81184753 Msa0429710:CDS 25.0%
! CGAAAAGTTGACATATTTAT+AGG - chr3_2:81184835-81184854 Msa0429710:CDS 25.0%
!!! TTTCTTTTTTGGTGAAGAAA+TGG + chr3_2:81184413-81184432 None:intergenic 25.0%
!! GGTCATAGATTTTTCTTTTT+TGG + chr3_2:81184424-81184443 None:intergenic 25.0%
! CTTGTCTTTATATTCTTCTT+TGG + chr3_2:81184466-81184485 None:intergenic 25.0%
! ACAAATGCATTATACTTCAA+AGG - chr3_2:81185803-81185822 Msa0429710:CDS 25.0%
! AATACTGTAGAAACACTAAT+TGG + chr3_2:81186241-81186260 None:intergenic 25.0%
! CTCTAATCAAAAACAAGAAA+GGG + chr3_2:81186387-81186406 None:intergenic 25.0%
! TCTCTAATCAAAAACAAGAA+AGG + chr3_2:81186388-81186407 None:intergenic 25.0%
!!! TTTCTTTTTTGGTGAAGAAA+TGG + chr3_2:81184413-81184432 None:intergenic 25.0%
!! GGTCATAGATTTTTCTTTTT+TGG + chr3_2:81184424-81184443 None:intergenic 25.0%
! CTTGTCTTTATATTCTTCTT+TGG + chr3_2:81184466-81184485 None:intergenic 25.0%
!! AACTGTTGCTATTCATTATA+TGG - chr3_2:81184734-81184753 Msa0429710:CDS 25.0%
! CGAAAAGTTGACATATTTAT+AGG - chr3_2:81184835-81184854 Msa0429710:CDS 25.0%
!!! TTTCTTTTTTGGTGAAGAAA+TGG + chr3_2:81184413-81184432 None:intergenic 25.0%
!! GGTCATAGATTTTTCTTTTT+TGG + chr3_2:81184424-81184443 None:intergenic 25.0%
! CTTGTCTTTATATTCTTCTT+TGG + chr3_2:81184466-81184485 None:intergenic 25.0%
! ACAAATGCATTATACTTCAA+AGG - chr3_2:81185803-81185822 Msa0429710:CDS 25.0%
! AATACTGTAGAAACACTAAT+TGG + chr3_2:81186241-81186260 None:intergenic 25.0%
! CTCTAATCAAAAACAAGAAA+GGG + chr3_2:81186387-81186406 None:intergenic 25.0%
! TCTCTAATCAAAAACAAGAA+AGG + chr3_2:81186388-81186407 None:intergenic 25.0%
!!! TTTTTTGGTGAAGAAATGGT+TGG + chr3_2:81184409-81184428 None:intergenic 30.0%
! ATTCTTCTTTGGAGAACAAA+TGG + chr3_2:81184455-81184474 None:intergenic 30.0%
AATAGCAACAGTTTCTTTGA+AGG + chr3_2:81184727-81184746 None:intergenic 30.0%
ATCACTTGATTTCACTAACA+AGG - chr3_2:81184767-81184786 Msa0429710:CDS 30.0%
TGCTAAGAAACGAGATATAT+TGG + chr3_2:81184925-81184944 None:intergenic 30.0%
!!! TTTTTTGGTGAAGAAATGGT+TGG + chr3_2:81184409-81184428 None:intergenic 30.0%
! ATTCTTCTTTGGAGAACAAA+TGG + chr3_2:81184455-81184474 None:intergenic 30.0%
AACTGTTGCTACTCATTATA+TGG - chr3_2:81185349-81185368 Msa0429710:intron 30.0%
AACCCAATCAGTGAATTTAT+GGG - chr3_2:81185703-81185722 Msa0429710:CDS 30.0%
! ATTATACTTCAAAGGAGCAT+GGG - chr3_2:81185811-81185830 Msa0429710:CDS 30.0%
TGAAATTTCTGATGTGTTGA+AGG - chr3_2:81186159-81186178 Msa0429710:CDS 30.0%
GTCATTGAAGTAAATGAAGA+AGG - chr3_2:81186271-81186290 Msa0429710:CDS 30.0%
TAATCAAAAACAAGAAAGGG+TGG + chr3_2:81186384-81186403 None:intergenic 30.0%
TTGATTAGAGAAGATTTGTC+TGG - chr3_2:81186397-81186416 Msa0429710:CDS 30.0%
!!! TTTTTTGGTGAAGAAATGGT+TGG + chr3_2:81184409-81184428 None:intergenic 30.0%
! ATTCTTCTTTGGAGAACAAA+TGG + chr3_2:81184455-81184474 None:intergenic 30.0%
AATAGCAACAGTTTCTTTGA+AGG + chr3_2:81184727-81184746 None:intergenic 30.0%
ATCACTTGATTTCACTAACA+AGG - chr3_2:81184767-81184786 Msa0429710:CDS 30.0%
TGCTAAGAAACGAGATATAT+TGG + chr3_2:81184925-81184944 None:intergenic 30.0%
!!! TTTTTTGGTGAAGAAATGGT+TGG + chr3_2:81184409-81184428 None:intergenic 30.0%
! ATTCTTCTTTGGAGAACAAA+TGG + chr3_2:81184455-81184474 None:intergenic 30.0%
AACTGTTGCTACTCATTATA+TGG - chr3_2:81185349-81185368 Msa0429710:intron 30.0%
AACCCAATCAGTGAATTTAT+GGG - chr3_2:81185703-81185722 Msa0429710:CDS 30.0%
! ATTATACTTCAAAGGAGCAT+GGG - chr3_2:81185811-81185830 Msa0429710:CDS 30.0%
TGAAATTTCTGATGTGTTGA+AGG - chr3_2:81186159-81186178 Msa0429710:CDS 30.0%
GTCATTGAAGTAAATGAAGA+AGG - chr3_2:81186271-81186290 Msa0429710:CDS 30.0%
TAATCAAAAACAAGAAAGGG+TGG + chr3_2:81186384-81186403 None:intergenic 30.0%
TTGATTAGAGAAGATTTGTC+TGG - chr3_2:81186397-81186416 Msa0429710:CDS 30.0%
! TTCTTTGGAGAACAAATGGT+TGG + chr3_2:81184451-81184470 None:intergenic 35.0%
! CAACAGTTTCTTTGAAGGAA+GGG + chr3_2:81184722-81184741 None:intergenic 35.0%
GAAATCAAGTGATTTGAGAG+TGG + chr3_2:81184760-81184779 None:intergenic 35.0%
CGAGATATATTGGACTTACT+TGG + chr3_2:81184915-81184934 None:intergenic 35.0%
!! ATTCTTCTGGTTTGAATGGT+TGG + chr3_2:81184969-81184988 None:intergenic 35.0%
AACCATTCAAACCAGAAGAA+TGG - chr3_2:81184968-81184987 Msa0429710:CDS 35.0%
!! ATCCATTCTTCTGGTTTGAA+TGG + chr3_2:81184973-81184992 None:intergenic 35.0%
! TTCTTTGGAGAACAAATGGT+TGG + chr3_2:81184451-81184470 None:intergenic 35.0%
! CAACAGTTTCTTTGAAGGAA+GGG + chr3_2:81184722-81184741 None:intergenic 35.0%
AGTAGCAACAGTTTCTTTGA+AGG + chr3_2:81185342-81185361 None:intergenic 35.0%
GAAATCAAGTGATTTGAGAG+TGG + chr3_2:81184760-81184779 None:intergenic 35.0%
CAACCCAATCAGTGAATTTA+TGG - chr3_2:81185702-81185721 Msa0429710:CDS 35.0%
! AGCCCATAAATTCACTGATT+GGG + chr3_2:81185708-81185727 None:intergenic 35.0%
! AGTGAATTTATGGGCTGAAA+AGG - chr3_2:81185712-81185731 Msa0429710:CDS 35.0%
! ATTCGTAATTTTCTTCCACC+TGG - chr3_2:81185749-81185768 Msa0429710:CDS 35.0%
! TTCGTAATTTTCTTCCACCT+GGG - chr3_2:81185750-81185769 Msa0429710:CDS 35.0%
CATTATACTTCAAAGGAGCA+TGG - chr3_2:81185810-81185829 Msa0429710:CDS 35.0%
! TACGATTTTCACCTTCTCAA+TGG - chr3_2:81185866-81185885 Msa0429710:CDS 35.0%
CTCTTCATGCTAGTCATAAA+GGG + chr3_2:81185908-81185927 None:intergenic 35.0%
! CAGTTTATCAGTGCTTTTGA+TGG - chr3_2:81185929-81185948 Msa0429710:intron 35.0%
TTTCTTCCAGATGCAAAAGA+TGG - chr3_2:81186016-81186035 Msa0429710:intron 35.0%
TTCTTCCAGATGCAAAAGAT+GGG - chr3_2:81186017-81186036 Msa0429710:intron 35.0%
GTTAGGTTTGATTGAGAAAG+CGG - chr3_2:81186042-81186061 Msa0429710:intron 35.0%
!! GTGGTTTTACCTTTCTCTTT+TGG - chr3_2:81186190-81186209 Msa0429710:CDS 35.0%
! GTTTTACCTTTCTCTTTTGG+AGG - chr3_2:81186193-81186212 Msa0429710:CDS 35.0%
TGGAGGTTTGACAAAAATAG+TGG - chr3_2:81186210-81186229 Msa0429710:CDS 35.0%
GCTACAAAGTCTATTGGTAT+CGG + chr3_2:81186358-81186377 None:intergenic 35.0%
TCTGGTACAATACTCTTTGT+TGG - chr3_2:81186415-81186434 Msa0429710:CDS 35.0%
CTGGTACAATACTCTTTGTT+GGG - chr3_2:81186416-81186435 Msa0429710:CDS 35.0%
! TTCTTTGGAGAACAAATGGT+TGG + chr3_2:81184451-81184470 None:intergenic 35.0%
! CAACAGTTTCTTTGAAGGAA+GGG + chr3_2:81184722-81184741 None:intergenic 35.0%
GAAATCAAGTGATTTGAGAG+TGG + chr3_2:81184760-81184779 None:intergenic 35.0%
CGAGATATATTGGACTTACT+TGG + chr3_2:81184915-81184934 None:intergenic 35.0%
!! ATTCTTCTGGTTTGAATGGT+TGG + chr3_2:81184969-81184988 None:intergenic 35.0%
AACCATTCAAACCAGAAGAA+TGG - chr3_2:81184968-81184987 Msa0429710:CDS 35.0%
!! ATCCATTCTTCTGGTTTGAA+TGG + chr3_2:81184973-81184992 None:intergenic 35.0%
! TTCTTTGGAGAACAAATGGT+TGG + chr3_2:81184451-81184470 None:intergenic 35.0%
! CAACAGTTTCTTTGAAGGAA+GGG + chr3_2:81184722-81184741 None:intergenic 35.0%
AGTAGCAACAGTTTCTTTGA+AGG + chr3_2:81185342-81185361 None:intergenic 35.0%
GAAATCAAGTGATTTGAGAG+TGG + chr3_2:81184760-81184779 None:intergenic 35.0%
CAACCCAATCAGTGAATTTA+TGG - chr3_2:81185702-81185721 Msa0429710:CDS 35.0%
! AGCCCATAAATTCACTGATT+GGG + chr3_2:81185708-81185727 None:intergenic 35.0%
! AGTGAATTTATGGGCTGAAA+AGG - chr3_2:81185712-81185731 Msa0429710:CDS 35.0%
! ATTCGTAATTTTCTTCCACC+TGG - chr3_2:81185749-81185768 Msa0429710:CDS 35.0%
! TTCGTAATTTTCTTCCACCT+GGG - chr3_2:81185750-81185769 Msa0429710:CDS 35.0%
CATTATACTTCAAAGGAGCA+TGG - chr3_2:81185810-81185829 Msa0429710:CDS 35.0%
! TACGATTTTCACCTTCTCAA+TGG - chr3_2:81185866-81185885 Msa0429710:CDS 35.0%
CTCTTCATGCTAGTCATAAA+GGG + chr3_2:81185908-81185927 None:intergenic 35.0%
! CAGTTTATCAGTGCTTTTGA+TGG - chr3_2:81185929-81185948 Msa0429710:intron 35.0%
TTTCTTCCAGATGCAAAAGA+TGG - chr3_2:81186016-81186035 Msa0429710:intron 35.0%
TTCTTCCAGATGCAAAAGAT+GGG - chr3_2:81186017-81186036 Msa0429710:intron 35.0%
GTTAGGTTTGATTGAGAAAG+CGG - chr3_2:81186042-81186061 Msa0429710:intron 35.0%
!! GTGGTTTTACCTTTCTCTTT+TGG - chr3_2:81186190-81186209 Msa0429710:CDS 35.0%
! GTTTTACCTTTCTCTTTTGG+AGG - chr3_2:81186193-81186212 Msa0429710:CDS 35.0%
TGGAGGTTTGACAAAAATAG+TGG - chr3_2:81186210-81186229 Msa0429710:CDS 35.0%
GCTACAAAGTCTATTGGTAT+CGG + chr3_2:81186358-81186377 None:intergenic 35.0%
TCTGGTACAATACTCTTTGT+TGG - chr3_2:81186415-81186434 Msa0429710:CDS 35.0%
CTGGTACAATACTCTTTGTT+GGG - chr3_2:81186416-81186435 Msa0429710:CDS 35.0%
! TGGTGAAGAAATGGTTGGTA+AGG + chr3_2:81184404-81184423 None:intergenic 40.0%
!! GACAGAAGCTGATTTTGTGT+GGG + chr3_2:81184555-81184574 None:intergenic 40.0%
AATCAGCTTCTGTCCTTTCT+CGG - chr3_2:81184561-81184580 Msa0429710:CDS 40.0%
TTTCTCGGATCAAAGTCCAT+CGG - chr3_2:81184576-81184595 Msa0429710:CDS 40.0%
!! TTTTTCAACCCAAACAGCGT+TGG + chr3_2:81184685-81184704 None:intergenic 40.0%
GAAGGAAGGGTAAACAGAAA+GGG + chr3_2:81184709-81184728 None:intergenic 40.0%
TGAAGGAAGGGTAAACAGAA+AGG + chr3_2:81184710-81184729 None:intergenic 40.0%
! GCAACAGTTTCTTTGAAGGA+AGG + chr3_2:81184723-81184742 None:intergenic 40.0%
GGAATCTCAGTTTCAATGCT+CGG + chr3_2:81184948-81184967 None:intergenic 40.0%
ACGAACGTAATCCATTCTTC+TGG + chr3_2:81184982-81185001 None:intergenic 40.0%
! TGGTGAAGAAATGGTTGGTA+AGG + chr3_2:81184404-81184423 None:intergenic 40.0%
!! GACAGAAGCTGATTTTGTGT+GGG + chr3_2:81184555-81184574 None:intergenic 40.0%
AATCAGCTTCTGTCCTTTCT+CGG - chr3_2:81184561-81184580 Msa0429710:CDS 40.0%
TTTCTCGGATCAAAGTCCAT+CGG - chr3_2:81184576-81184595 Msa0429710:CDS 40.0%
!! TTTTTCAACCCAAACAGCGT+TGG + chr3_2:81184685-81184704 None:intergenic 40.0%
GAAGGAAGGGTAAACAGAAA+GGG + chr3_2:81184709-81184728 None:intergenic 40.0%
TGAAGGAAGGGTAAACAGAA+AGG + chr3_2:81184710-81184729 None:intergenic 40.0%
! GCAACAGTTTCTTTGAAGGA+AGG + chr3_2:81184723-81184742 None:intergenic 40.0%
CAGCCCATAAATTCACTGAT+TGG + chr3_2:81185709-81185728 None:intergenic 40.0%
GTTAAGCTATTAATCGACCC+AGG + chr3_2:81185770-81185789 None:intergenic 40.0%
CCTCTTCATGCTAGTCATAA+AGG + chr3_2:81185909-81185928 None:intergenic 40.0%
CCTTTATGACTAGCATGAAG+AGG - chr3_2:81185906-81185925 Msa0429710:intron 40.0%
!! CAGTGCTTTTGATGGCTTTA+AGG - chr3_2:81185937-81185956 Msa0429710:intron 40.0%
CTTGCTTGTAAGGAAGACTA+AGG + chr3_2:81185964-81185983 None:intergenic 40.0%
! CTTAGTCTTCCTTACAAGCA+AGG - chr3_2:81185962-81185981 Msa0429710:intron 40.0%
TCATCTTCACCTTGCTTGTA+AGG + chr3_2:81185974-81185993 None:intergenic 40.0%
! AACAACCCATCTTTTGCATC+TGG + chr3_2:81186025-81186044 None:intergenic 40.0%
!! GATGCAAAAGATGGGTTGTT+AGG - chr3_2:81186025-81186044 Msa0429710:intron 40.0%
TATGAGGAAGCTTGTCTTCT+AGG + chr3_2:81186084-81186103 None:intergenic 40.0%
CAAGCTTCCTCATACAAAAG+TGG - chr3_2:81186090-81186109 Msa0429710:CDS 40.0%
TCTGATGTGTTGAAGGAGTT+AGG - chr3_2:81186166-81186185 Msa0429710:CDS 40.0%
GTCAAACCTCCAAAAGAGAA+AGG + chr3_2:81186202-81186221 None:intergenic 40.0%
TGGTCAGCTACAAAGTCTAT+TGG + chr3_2:81186364-81186383 None:intergenic 40.0%
! TGGTGAAGAAATGGTTGGTA+AGG + chr3_2:81184404-81184423 None:intergenic 40.0%
!! GACAGAAGCTGATTTTGTGT+GGG + chr3_2:81184555-81184574 None:intergenic 40.0%
AATCAGCTTCTGTCCTTTCT+CGG - chr3_2:81184561-81184580 Msa0429710:CDS 40.0%
TTTCTCGGATCAAAGTCCAT+CGG - chr3_2:81184576-81184595 Msa0429710:CDS 40.0%
!! TTTTTCAACCCAAACAGCGT+TGG + chr3_2:81184685-81184704 None:intergenic 40.0%
GAAGGAAGGGTAAACAGAAA+GGG + chr3_2:81184709-81184728 None:intergenic 40.0%
TGAAGGAAGGGTAAACAGAA+AGG + chr3_2:81184710-81184729 None:intergenic 40.0%
! GCAACAGTTTCTTTGAAGGA+AGG + chr3_2:81184723-81184742 None:intergenic 40.0%
GGAATCTCAGTTTCAATGCT+CGG + chr3_2:81184948-81184967 None:intergenic 40.0%
ACGAACGTAATCCATTCTTC+TGG + chr3_2:81184982-81185001 None:intergenic 40.0%
! TGGTGAAGAAATGGTTGGTA+AGG + chr3_2:81184404-81184423 None:intergenic 40.0%
!! GACAGAAGCTGATTTTGTGT+GGG + chr3_2:81184555-81184574 None:intergenic 40.0%
AATCAGCTTCTGTCCTTTCT+CGG - chr3_2:81184561-81184580 Msa0429710:CDS 40.0%
TTTCTCGGATCAAAGTCCAT+CGG - chr3_2:81184576-81184595 Msa0429710:CDS 40.0%
!! TTTTTCAACCCAAACAGCGT+TGG + chr3_2:81184685-81184704 None:intergenic 40.0%
GAAGGAAGGGTAAACAGAAA+GGG + chr3_2:81184709-81184728 None:intergenic 40.0%
TGAAGGAAGGGTAAACAGAA+AGG + chr3_2:81184710-81184729 None:intergenic 40.0%
! GCAACAGTTTCTTTGAAGGA+AGG + chr3_2:81184723-81184742 None:intergenic 40.0%
CAGCCCATAAATTCACTGAT+TGG + chr3_2:81185709-81185728 None:intergenic 40.0%
GTTAAGCTATTAATCGACCC+AGG + chr3_2:81185770-81185789 None:intergenic 40.0%
CCTCTTCATGCTAGTCATAA+AGG + chr3_2:81185909-81185928 None:intergenic 40.0%
CCTTTATGACTAGCATGAAG+AGG - chr3_2:81185906-81185925 Msa0429710:intron 40.0%
!! CAGTGCTTTTGATGGCTTTA+AGG - chr3_2:81185937-81185956 Msa0429710:intron 40.0%
CTTGCTTGTAAGGAAGACTA+AGG + chr3_2:81185964-81185983 None:intergenic 40.0%
! CTTAGTCTTCCTTACAAGCA+AGG - chr3_2:81185962-81185981 Msa0429710:intron 40.0%
TCATCTTCACCTTGCTTGTA+AGG + chr3_2:81185974-81185993 None:intergenic 40.0%
! AACAACCCATCTTTTGCATC+TGG + chr3_2:81186025-81186044 None:intergenic 40.0%
!! GATGCAAAAGATGGGTTGTT+AGG - chr3_2:81186025-81186044 Msa0429710:intron 40.0%
TATGAGGAAGCTTGTCTTCT+AGG + chr3_2:81186084-81186103 None:intergenic 40.0%
CAAGCTTCCTCATACAAAAG+TGG - chr3_2:81186090-81186109 Msa0429710:CDS 40.0%
TCTGATGTGTTGAAGGAGTT+AGG - chr3_2:81186166-81186185 Msa0429710:CDS 40.0%
GTCAAACCTCCAAAAGAGAA+AGG + chr3_2:81186202-81186221 None:intergenic 40.0%
TGGTCAGCTACAAAGTCTAT+TGG + chr3_2:81186364-81186383 None:intergenic 40.0%
!! GGACAGAAGCTGATTTTGTG+TGG + chr3_2:81184556-81184575 None:intergenic 45.0%
! GATGGACTTTGATCCGAGAA+AGG + chr3_2:81184577-81184596 None:intergenic 45.0%
GAGAAGATAGGGAGTTGAGA+TGG + chr3_2:81184602-81184621 None:intergenic 45.0%
!! GGACAGAAGCTGATTTTGTG+TGG + chr3_2:81184556-81184575 None:intergenic 45.0%
! GATGGACTTTGATCCGAGAA+AGG + chr3_2:81184577-81184596 None:intergenic 45.0%
GAGAAGATAGGGAGTTGAGA+TGG + chr3_2:81184602-81184621 None:intergenic 45.0%
ACTACCATACAACAGTTGCG+AGG + chr3_2:81185511-81185530 None:intergenic 45.0%
TGGGCTGAAAAGGAGACAAA+AGG - chr3_2:81185722-81185741 Msa0429710:CDS 45.0%
GGGCTGAAAAGGAGACAAAA+GGG - chr3_2:81185723-81185742 Msa0429710:CDS 45.0%
AAGCTATTAATCGACCCAGG+TGG + chr3_2:81185767-81185786 None:intergenic 45.0%
GGAGCAATCGTTTGATGCTT+CGG - chr3_2:81185832-81185851 Msa0429710:CDS 45.0%
CATAAAGGGAACCTTGACTG+AGG + chr3_2:81185894-81185913 None:intergenic 45.0%
GCTTCCTCATACAAAAGTGG+TGG - chr3_2:81186093-81186112 Msa0429710:CDS 45.0%
!! CCTACCACCACTTTTGTATG+AGG + chr3_2:81186100-81186119 None:intergenic 45.0%
CCTCATACAAAAGTGGTGGT+AGG - chr3_2:81186097-81186116 Msa0429710:CDS 45.0%
TGTGTTGAAGGAGTTAGGAG+TGG - chr3_2:81186171-81186190 Msa0429710:CDS 45.0%
CATCCCAATTCCAGTGAATG+CGG + chr3_2:81186308-81186327 None:intergenic 45.0%
! GGTATCGGAGTTGAAGTGTA+TGG + chr3_2:81186343-81186362 None:intergenic 45.0%
!! GGACAGAAGCTGATTTTGTG+TGG + chr3_2:81184556-81184575 None:intergenic 45.0%
! GATGGACTTTGATCCGAGAA+AGG + chr3_2:81184577-81184596 None:intergenic 45.0%
GAGAAGATAGGGAGTTGAGA+TGG + chr3_2:81184602-81184621 None:intergenic 45.0%
!! GGACAGAAGCTGATTTTGTG+TGG + chr3_2:81184556-81184575 None:intergenic 45.0%
! GATGGACTTTGATCCGAGAA+AGG + chr3_2:81184577-81184596 None:intergenic 45.0%
GAGAAGATAGGGAGTTGAGA+TGG + chr3_2:81184602-81184621 None:intergenic 45.0%
ACTACCATACAACAGTTGCG+AGG + chr3_2:81185511-81185530 None:intergenic 45.0%
TGGGCTGAAAAGGAGACAAA+AGG - chr3_2:81185722-81185741 Msa0429710:CDS 45.0%
GGGCTGAAAAGGAGACAAAA+GGG - chr3_2:81185723-81185742 Msa0429710:CDS 45.0%
AAGCTATTAATCGACCCAGG+TGG + chr3_2:81185767-81185786 None:intergenic 45.0%
GGAGCAATCGTTTGATGCTT+CGG - chr3_2:81185832-81185851 Msa0429710:CDS 45.0%
CATAAAGGGAACCTTGACTG+AGG + chr3_2:81185894-81185913 None:intergenic 45.0%
GCTTCCTCATACAAAAGTGG+TGG - chr3_2:81186093-81186112 Msa0429710:CDS 45.0%
!! CCTACCACCACTTTTGTATG+AGG + chr3_2:81186100-81186119 None:intergenic 45.0%
CCTCATACAAAAGTGGTGGT+AGG - chr3_2:81186097-81186116 Msa0429710:CDS 45.0%
TGTGTTGAAGGAGTTAGGAG+TGG - chr3_2:81186171-81186190 Msa0429710:CDS 45.0%
CATCCCAATTCCAGTGAATG+CGG + chr3_2:81186308-81186327 None:intergenic 45.0%
! GGTATCGGAGTTGAAGTGTA+TGG + chr3_2:81186343-81186362 None:intergenic 45.0%
GGAGAACAAATGGTTGGCGA+TGG + chr3_2:81184445-81184464 None:intergenic 50.0%
GGAAGCGACGTGAGAAGATA+GGG + chr3_2:81184613-81184632 None:intergenic 50.0%
! AGCAGCGTCTTTGAGAACAG+CGG + chr3_2:81184634-81184653 None:intergenic 50.0%
TGTCTTCTGCCAACGCTGTT+TGG - chr3_2:81184673-81184692 Msa0429710:CDS 50.0%
GTCTTCTGCCAACGCTGTTT+GGG - chr3_2:81184674-81184693 Msa0429710:CDS 50.0%
GGAGAACAAATGGTTGGCGA+TGG + chr3_2:81184445-81184464 None:intergenic 50.0%
GGAAGCGACGTGAGAAGATA+GGG + chr3_2:81184613-81184632 None:intergenic 50.0%
! AGCAGCGTCTTTGAGAACAG+CGG + chr3_2:81184634-81184653 None:intergenic 50.0%
TGTCTTCTGCCAACGCTGTT+TGG - chr3_2:81184673-81184692 Msa0429710:CDS 50.0%
GTCTTCTGCCAACGCTGTTT+GGG - chr3_2:81184674-81184693 Msa0429710:CDS 50.0%
CATACAACAGTTGCGAGGCA+TGG + chr3_2:81185506-81185525 None:intergenic 50.0%
CATGCCTCGCAACTGTTGTA+TGG - chr3_2:81185504-81185523 Msa0429710:CDS 50.0%
!! TGACTGAGGTGCCATTGAGA+AGG + chr3_2:81185880-81185899 None:intergenic 50.0%
TCTCAATGGCACCTCAGTCA+AGG - chr3_2:81185880-81185899 Msa0429710:CDS 50.0%
ACTAAAGCTGCCGCATTCAC+TGG - chr3_2:81186295-81186314 Msa0429710:CDS 50.0%
CTGCCGCATTCACTGGAATT+GGG - chr3_2:81186302-81186321 Msa0429710:CDS 50.0%
CGCATTCACTGGAATTGGGA+TGG - chr3_2:81186306-81186325 Msa0429710:CDS 50.0%
TTCACTGGAATTGGGATGGC+TGG - chr3_2:81186310-81186329 Msa0429710:CDS 50.0%
GGAGAACAAATGGTTGGCGA+TGG + chr3_2:81184445-81184464 None:intergenic 50.0%
GGAAGCGACGTGAGAAGATA+GGG + chr3_2:81184613-81184632 None:intergenic 50.0%
! AGCAGCGTCTTTGAGAACAG+CGG + chr3_2:81184634-81184653 None:intergenic 50.0%
TGTCTTCTGCCAACGCTGTT+TGG - chr3_2:81184673-81184692 Msa0429710:CDS 50.0%
GTCTTCTGCCAACGCTGTTT+GGG - chr3_2:81184674-81184693 Msa0429710:CDS 50.0%
GGAGAACAAATGGTTGGCGA+TGG + chr3_2:81184445-81184464 None:intergenic 50.0%
GGAAGCGACGTGAGAAGATA+GGG + chr3_2:81184613-81184632 None:intergenic 50.0%
! AGCAGCGTCTTTGAGAACAG+CGG + chr3_2:81184634-81184653 None:intergenic 50.0%
TGTCTTCTGCCAACGCTGTT+TGG - chr3_2:81184673-81184692 Msa0429710:CDS 50.0%
GTCTTCTGCCAACGCTGTTT+GGG - chr3_2:81184674-81184693 Msa0429710:CDS 50.0%
CATACAACAGTTGCGAGGCA+TGG + chr3_2:81185506-81185525 None:intergenic 50.0%
CATGCCTCGCAACTGTTGTA+TGG - chr3_2:81185504-81185523 Msa0429710:CDS 50.0%
!! TGACTGAGGTGCCATTGAGA+AGG + chr3_2:81185880-81185899 None:intergenic 50.0%
TCTCAATGGCACCTCAGTCA+AGG - chr3_2:81185880-81185899 Msa0429710:CDS 50.0%
ACTAAAGCTGCCGCATTCAC+TGG - chr3_2:81186295-81186314 Msa0429710:CDS 50.0%
CTGCCGCATTCACTGGAATT+GGG - chr3_2:81186302-81186321 Msa0429710:CDS 50.0%
CGCATTCACTGGAATTGGGA+TGG - chr3_2:81186306-81186325 Msa0429710:CDS 50.0%
TTCACTGGAATTGGGATGGC+TGG - chr3_2:81186310-81186329 Msa0429710:CDS 50.0%
! AGCGCAGTTTGGAGCGACAA+TGG + chr3_2:81184504-81184523 None:intergenic 55.0%
!! TCTCCTCGCTGTTGGTTCTG+AGG - chr3_2:81184527-81184546 Msa0429710:CDS 55.0%
!! CTCCTCGCTGTTGGTTCTGA+GGG - chr3_2:81184528-81184547 Msa0429710:CDS 55.0%
TAGGGAGTTGAGATGGCCGA+TGG + chr3_2:81184595-81184614 None:intergenic 55.0%
CGGAAGCGACGTGAGAAGAT+AGG + chr3_2:81184614-81184633 None:intergenic 55.0%
AGAAGACAAGCATGGACCGC+CGG + chr3_2:81184661-81184680 None:intergenic 55.0%
! GCGTTGGCAGAAGACAAGCA+TGG + chr3_2:81184669-81184688 None:intergenic 55.0%
! AGCGCAGTTTGGAGCGACAA+TGG + chr3_2:81184504-81184523 None:intergenic 55.0%
!! TCTCCTCGCTGTTGGTTCTG+AGG - chr3_2:81184527-81184546 Msa0429710:CDS 55.0%
!! CTCCTCGCTGTTGGTTCTGA+GGG - chr3_2:81184528-81184547 Msa0429710:CDS 55.0%
TAGGGAGTTGAGATGGCCGA+TGG + chr3_2:81184595-81184614 None:intergenic 55.0%
CGGAAGCGACGTGAGAAGAT+AGG + chr3_2:81184614-81184633 None:intergenic 55.0%
AGAAGACAAGCATGGACCGC+CGG + chr3_2:81184661-81184680 None:intergenic 55.0%
! GCGTTGGCAGAAGACAAGCA+TGG + chr3_2:81184669-81184688 None:intergenic 55.0%
GCTGCCGCATTCACTGGAAT+TGG - chr3_2:81186301-81186320 Msa0429710:CDS 55.0%
CAAGTGCATAATCCCCTCGC+TGG - chr3_2:81186439-81186458 Msa0429710:CDS 55.0%
! AGCGCAGTTTGGAGCGACAA+TGG + chr3_2:81184504-81184523 None:intergenic 55.0%
!! TCTCCTCGCTGTTGGTTCTG+AGG - chr3_2:81184527-81184546 Msa0429710:CDS 55.0%
!! CTCCTCGCTGTTGGTTCTGA+GGG - chr3_2:81184528-81184547 Msa0429710:CDS 55.0%
TAGGGAGTTGAGATGGCCGA+TGG + chr3_2:81184595-81184614 None:intergenic 55.0%
CGGAAGCGACGTGAGAAGAT+AGG + chr3_2:81184614-81184633 None:intergenic 55.0%
AGAAGACAAGCATGGACCGC+CGG + chr3_2:81184661-81184680 None:intergenic 55.0%
! GCGTTGGCAGAAGACAAGCA+TGG + chr3_2:81184669-81184688 None:intergenic 55.0%
! AGCGCAGTTTGGAGCGACAA+TGG + chr3_2:81184504-81184523 None:intergenic 55.0%
!! TCTCCTCGCTGTTGGTTCTG+AGG - chr3_2:81184527-81184546 Msa0429710:CDS 55.0%
!! CTCCTCGCTGTTGGTTCTGA+GGG - chr3_2:81184528-81184547 Msa0429710:CDS 55.0%
TAGGGAGTTGAGATGGCCGA+TGG + chr3_2:81184595-81184614 None:intergenic 55.0%
CGGAAGCGACGTGAGAAGAT+AGG + chr3_2:81184614-81184633 None:intergenic 55.0%
AGAAGACAAGCATGGACCGC+CGG + chr3_2:81184661-81184680 None:intergenic 55.0%
! GCGTTGGCAGAAGACAAGCA+TGG + chr3_2:81184669-81184688 None:intergenic 55.0%
GCTGCCGCATTCACTGGAAT+TGG - chr3_2:81186301-81186320 Msa0429710:CDS 55.0%
CAAGTGCATAATCCCCTCGC+TGG - chr3_2:81186439-81186458 Msa0429710:CDS 55.0%
CGAGGAGACTGAGCGCAGTT+TGG + chr3_2:81184515-81184534 None:intergenic 60.0%
GACCCTCAGAACCAACAGCG+AGG + chr3_2:81184533-81184552 None:intergenic 60.0%
CTCAAAGACGCTGCTCCTGC+CGG - chr3_2:81184639-81184658 Msa0429710:CDS 60.0%
CGAGGAGACTGAGCGCAGTT+TGG + chr3_2:81184515-81184534 None:intergenic 60.0%
GACCCTCAGAACCAACAGCG+AGG + chr3_2:81184533-81184552 None:intergenic 60.0%
CTCAAAGACGCTGCTCCTGC+CGG - chr3_2:81184639-81184658 Msa0429710:CDS 60.0%
CGAGGAGACTGAGCGCAGTT+TGG + chr3_2:81184515-81184534 None:intergenic 60.0%
GACCCTCAGAACCAACAGCG+AGG + chr3_2:81184533-81184552 None:intergenic 60.0%
CTCAAAGACGCTGCTCCTGC+CGG - chr3_2:81184639-81184658 Msa0429710:CDS 60.0%
CGAGGAGACTGAGCGCAGTT+TGG + chr3_2:81184515-81184534 None:intergenic 60.0%
GACCCTCAGAACCAACAGCG+AGG + chr3_2:81184533-81184552 None:intergenic 60.0%
CTCAAAGACGCTGCTCCTGC+CGG - chr3_2:81184639-81184658 Msa0429710:CDS 60.0%
GCGCTCAGTCTCCTCGCTGT+TGG - chr3_2:81184519-81184538 Msa0429710:CDS 65.0%
AAAGACGCTGCTCCTGCCGG+CGG - chr3_2:81184642-81184661 Msa0429710:CDS 65.0%
GCGCTCAGTCTCCTCGCTGT+TGG - chr3_2:81184519-81184538 Msa0429710:CDS 65.0%
AAAGACGCTGCTCCTGCCGG+CGG - chr3_2:81184642-81184661 Msa0429710:CDS 65.0%
GCGCTCAGTCTCCTCGCTGT+TGG - chr3_2:81184519-81184538 Msa0429710:CDS 65.0%
AAAGACGCTGCTCCTGCCGG+CGG - chr3_2:81184642-81184661 Msa0429710:CDS 65.0%
GCGCTCAGTCTCCTCGCTGT+TGG - chr3_2:81184519-81184538 Msa0429710:CDS 65.0%
AAAGACGCTGCTCCTGCCGG+CGG - chr3_2:81184642-81184661 Msa0429710:CDS 65.0%
GACAAGCATGGACCGCCGGC+AGG + chr3_2:81184657-81184676 None:intergenic 70.0%
GACAAGCATGGACCGCCGGC+AGG + chr3_2:81184657-81184676 None:intergenic 70.0%
GACAAGCATGGACCGCCGGC+AGG + chr3_2:81184657-81184676 None:intergenic 70.0%
GACAAGCATGGACCGCCGGC+AGG + chr3_2:81184657-81184676 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 81184372 81186465 81184372 ID=Msa0429710;Name=Msa0429710
chr3_2 mRNA 81184372 81186465 81184372 ID=Msa0429710-mRNA-1;Parent=Msa0429710;Name=Msa0429710-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.5|0|1|1|1|3|0|557
chr3_2 exon 81186049 81186465 81186049 ID=Msa0429710-mRNA-1:exon:22377;Parent=Msa0429710-mRNA-1
chr3_2 exon 81185434 81185916 81185434 ID=Msa0429710-mRNA-1:exon:22376;Parent=Msa0429710-mRNA-1
chr3_2 exon 81184372 81185145 81184372 ID=Msa0429710-mRNA-1:exon:22375;Parent=Msa0429710-mRNA-1
chr3_2 CDS 81186049 81186465 81186049 ID=Msa0429710-mRNA-1:cds;Parent=Msa0429710-mRNA-1
chr3_2 CDS 81185434 81185916 81185434 ID=Msa0429710-mRNA-1:cds;Parent=Msa0429710-mRNA-1
chr3_2 CDS 81184372 81185145 81184372 ID=Msa0429710-mRNA-1:cds;Parent=Msa0429710-mRNA-1
Gene Sequence

>Msa0429710

ATGGATTACGTTCGTGAATCAATCAAAAACCTTACCAACCATTTCTTCACCAAAAAAGAAAAATCTATGACCATCGCCAACCATTTGTTCTCCAAAGAAGAATATAAAGACAAGAATGTAGTGTTTTCGCCATTGTCGCTCCAAACTGCGCTCAGTCTCCTCGCTGTTGGTTCTGAGGGTCCCACACAAAATCAGCTTCTGTCCTTTCTCGGATCAAAGTCCATCGGCCATCTCAACTCCCTATCTTCTCACGTCGCTTCCGCTGTTCTCAAAGACGCTGCTCCTGCCGGCGGTCCATGCTTGTCTTCTGCCAACGCTGTTTGGGTTGAAAAAACCCTTTCTGTTTACCCTTCCTTCAAAGAAACTGTTGCTATTCATTATATGGCCACTCTCAAATCACTTGATTTCACTAACAAGTCCAATATATCTCGTTTCTTAGCACCGAGCATTGAAACTGAGATTCCAACCATTCAAACCAGAAGAATGGATTACGTTCGTGAATCAATCAAAAACCTTACCAACCATTTCTTCACCAAAAAAGAAAAATCTATGACCATCGCCAACCATTTGTTCTCCAAAGAAGAATATAAAGACAAGAATGTAGTGTTTTCGCCATTGTCGCTCCAAACTGCGCTCAGTCTCCTCGCTGTTGGTTCTGAGGGTCCCACACAAAATCAGCTTCTGTCCTTTCTCGGATCAAAGTCCATCGGCCATCTCAACTCCCTATCTTCTCACGTCGCTTCCGCTGTTCTCAAAGACGCTGCTCCTGCCGGCGGTCCATGCTTGTCTTCTGCCAACGCTGTTTGGGTTGAAAAAACCCTTTCTGTTTACCCTTCCTTCAAAGAAACTGTTGCTACTCATTATATGGCCACTCTCAAATCACTTGATTTCATTAACAATGCTAATAAAGCAACCCAATCAGTGAATTTATGGGCTGAAAAGGAGACAAAAGGGCTAATTCGTAATTTTCTTCCACCTGGGTCGATTAATAGCTTAACTAGACTCATCTTGACAAATGCATTATACTTCAAAGGAGCATGGGAGCAATCGTTTGATGCTTCGGAAACAAAAGACTACGATTTTCACCTTCTCAATGGCACCTCAGTCAAGGTTCCCTTTATGACTAGCATGAAGAGGCAGTTTATCAGTGCTTTTGATGGCTTTAAGGTCCTTAGTCTTCCTTACAAGCAAGGTGAAGATGAGCGACAATTCTCAATGTACGTTTTTCTTCCAGATGCAAAAGATGGGTTGTTAGGTTTGATTGAGAAAGCGGCTACTGAGTCTAACTTCCTAGAAGACAAGCTTCCTCATACAAAAGTGGTGGTAGGTGATTTCAGAATTCCAAGATTCAATATTTCTTTTGAGCTTGAAATTTCTGATGTGTTGAAGGAGTTAGGAGTGGTTTTACCTTTCTCTTTTGGAGGTTTGACAAAAATAGTGGACTCTCCAATTAGTGTTTCTACAGTATTGCAAAAGTCTGTCATTGAAGTAAATGAAGAAGGCACTAAAGCTGCCGCATTCACTGGAATTGGGATGGCTGGATGTTGTCCATACACTTCAACTCCGATACCAATAGACTTTGTAGCTGACCACCCTTTCTTGTTTTTGATTAGAGAAGATTTGTCTGGTACAATACTCTTTGTTGGGCAAGTGCATAATCCCCTCGCTGGATGA
Protein sequence

>Msa0429710

MDYVRESIKNLTNHFFTKKEKSMTIANHLFSKEEYKDKNVVFSPLSLQTALSLLAVGSEGPTQNQLLSFLGSKSIGHLNSLSSHVASAVLKDAAPAGGPCLSSANAVWVEKTLSVYPSFKETVAIHYMATLKSLDFTNKSNISRFLAPSIETEIPTIQTRRMDYVRESIKNLTNHFFTKKEKSMTIANHLFSKEEYKDKNVVFSPLSLQTALSLLAVGSEGPTQNQLLSFLGSKSIGHLNSLSSHVASAVLKDAAPAGGPCLSSANAVWVEKTLSVYPSFKETVATHYMATLKSLDFINNANKATQSVNLWAEKETKGLIRNFLPPGSINSLTRLILTNALYFKGAWEQSFDASETKDYDFHLLNGTSVKVPFMTSMKRQFISAFDGFKVLSLPYKQGEDERQFSMYVFLPDAKDGLLGLIEKAATESNFLEDKLPHTKVVVGDFRIPRFNISFELEISDVLKELGVVLPFSFGGLTKIVDSPISVSTVLQKSVIEVNEEGTKAAAFTGIGMAGCCPYTSTPIPIDFVADHPFLFLIREDLSGTILFVGQVHNPLAG*