AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0459730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459730 MtrunA17_Chr3g0108261 100.000 148 0 0 1 148 1 148 5.28e-110 308
Msa0459730 MtrunA17_Chr5g0432831 98.649 148 2 0 1 148 1 148 1.15e-108 305
Msa0459730 MtrunA17_Chr7g0275361 97.297 148 4 0 1 148 1 148 2.12e-107 302
Msa0459730 MtrunA17_Chr8g0369311 93.919 148 9 0 1 148 57 204 1.02e-103 295
Msa0459730 MtrunA17_Chr1g0154011 91.892 148 12 0 1 148 1 148 4.66e-103 291
Msa0459730 MtrunA17_Chr4g0037011 91.892 148 12 0 1 148 1 148 5.03e-103 291
Msa0459730 MtrunA17_Chr8g0378601 51.034 145 71 0 2 146 23 167 5.60e-55 170
Msa0459730 MtrunA17_Chr2g0316201 48.966 145 74 0 4 148 8 152 1.14e-47 151
Msa0459730 MtrunA17_Chr2g0316211 48.611 144 74 0 5 148 9 152 1.22e-46 148
Msa0459730 MtrunA17_Chr4g0060351 45.890 146 74 1 2 142 5 150 3.03e-45 145
Msa0459730 MtrunA17_Chr8g0362321 45.205 146 75 1 2 142 5 150 4.64e-45 144
Msa0459730 MtrunA17_Chr3g0131871 48.611 144 72 2 5 146 6 149 3.81e-42 138
Msa0459730 MtrunA17_Chr3g0139901 40.136 147 84 3 5 147 7 153 3.41e-34 117
Msa0459730 MtrunA17_Chr4g0038891 44.355 124 69 0 4 127 35 158 6.03e-34 117
Msa0459730 MtrunA17_Chr1g0199951 45.669 127 68 1 3 128 43 169 5.00e-33 115
Msa0459730 MtrunA17_Chr5g0396451 41.406 128 75 0 4 131 35 162 3.75e-32 112
Msa0459730 MtrunA17_Chr4g0053191 36.842 152 89 2 1 145 4 155 1.66e-30 107
Msa0459730 MtrunA17_Chr4g0031181 35.821 134 72 2 15 134 18 151 2.23e-27 100
Msa0459730 MtrunA17_Chr5g0401831 33.553 152 94 2 1 145 4 155 3.44e-27 99.4
Msa0459730 MtrunA17_Chr8g0348701 37.398 123 75 2 26 146 25 147 7.68e-27 99.4
Msa0459730 MtrunA17_Chr5g0403591 36.765 136 83 2 5 139 33 166 2.15e-26 98.2
Msa0459730 MtrunA17_Chr7g0254701 34.965 143 93 0 4 146 13 155 2.55e-25 97.4
Msa0459730 MtrunA17_Chr8g0387131 35.036 137 87 2 12 146 11 147 4.13e-25 94.7
Msa0459730 MtrunA17_Chr2g0328001 34.028 144 81 2 15 144 18 161 6.95e-25 93.6
Msa0459730 MtrunA17_Chr4g0059291 30.894 123 83 2 26 146 25 147 2.41e-22 87.8
Msa0459730 MtrunA17_Chr8g0372391 38.835 103 61 2 1 102 15 116 3.67e-19 79.0
Msa0459730 MtrunA17_Chr4g0068301 34.454 119 68 4 2 114 11 125 2.13e-18 79.3
Msa0459730 MtrunA17_Chr8g0376111 31.373 153 87 5 4 144 43 189 3.56e-18 79.3
Msa0459730 MtrunA17_Chr5g0404931 36.893 103 63 2 1 102 15 116 5.90e-18 75.5
Msa0459730 MtrunA17_Chr7g0233631 36.885 122 67 3 4 115 38 159 3.02e-17 76.6
Msa0459730 MtrunA17_Chr7g0233611 36.885 122 67 3 4 115 38 159 6.76e-17 75.5
Msa0459730 MtrunA17_Chr8g0345331 32.479 117 70 3 4 115 8 120 5.17e-16 72.0
Msa0459730 MtrunA17_Chr7g0222941 30.894 123 75 3 3 115 90 212 7.54e-15 68.9
Msa0459730 MtrunA17_Chr4g0047301 30.667 150 91 4 4 143 672 818 1.20e-14 70.1
Msa0459730 MtrunA17_Chr4g0045101 32.787 122 72 3 4 115 37 158 3.41e-14 68.2
Msa0459730 MtrunA17_Chr2g0282211 28.289 152 96 4 3 144 681 829 1.05e-13 67.4
Msa0459730 MtrunA17_Chr1g0186291 29.293 99 56 2 43 140 30 115 8.78e-13 61.6
Msa0459730 MtrunA17_Chr2g0317631 30.081 123 76 3 3 115 306 428 1.24e-12 64.3
Msa0459730 MtrunA17_Chr8g0376201 31.707 123 74 3 3 115 192 314 2.39e-12 63.2
Msa0459730 MtrunA17_Chr1g0198021 28.455 123 78 3 3 115 137 259 3.16e-12 62.8
Msa0459730 MtrunA17_Chr1g0211131 29.508 122 76 3 4 115 823 944 5.52e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459730 AT5G41700.5 96.622 148 5 0 1 148 1 148 2.45e-107 302
Msa0459730 AT5G41700.2 96.622 148 5 0 1 148 1 148 2.45e-107 302
Msa0459730 AT5G41700.1 96.622 148 5 0 1 148 1 148 2.45e-107 302
Msa0459730 AT1G64230.2 95.946 148 6 0 1 148 1 148 2.77e-107 302
Msa0459730 AT1G64230.1 95.946 148 6 0 1 148 1 148 2.77e-107 302
Msa0459730 AT5G53300.2 95.946 148 6 0 1 148 1 148 8.38e-107 301
Msa0459730 AT5G53300.4 95.946 148 6 0 1 148 1 148 8.38e-107 301
Msa0459730 AT5G53300.1 95.946 148 6 0 1 148 1 148 8.38e-107 301
Msa0459730 AT4G27960.1 95.270 148 7 0 1 148 1 148 1.73e-106 300
Msa0459730 AT4G27960.2 95.270 148 7 0 1 148 31 178 5.81e-106 300
Msa0459730 AT3G08690.2 93.919 148 9 0 1 148 1 148 6.12e-105 296
Msa0459730 AT3G08690.1 93.919 148 9 0 1 148 1 148 6.12e-105 296
Msa0459730 AT5G41700.4 95.302 149 6 1 1 148 1 149 1.58e-104 295
Msa0459730 AT1G64230.3 89.809 157 7 1 1 148 1 157 1.05e-102 291
Msa0459730 AT5G56150.1 89.189 148 16 0 1 148 1 148 1.26e-100 285
Msa0459730 AT2G16740.1 87.755 147 18 0 1 147 1 147 7.55e-99 281
Msa0459730 AT1G64230.5 74.211 190 7 1 1 148 1 190 2.92e-98 281
Msa0459730 AT1G64230.4 74.211 190 7 1 1 148 1 190 2.92e-98 281
Msa0459730 AT3G08700.2 79.195 149 30 1 1 148 1 149 3.19e-87 251
Msa0459730 AT3G08700.1 79.195 149 30 1 1 148 1 149 3.19e-87 251
Msa0459730 AT5G41700.3 96.154 104 4 0 1 104 1 104 1.37e-72 214
Msa0459730 AT5G53300.3 93.578 109 5 1 1 107 1 109 9.94e-72 211
Msa0459730 AT5G56150.2 87.379 103 13 0 1 103 1 103 3.66e-66 197
Msa0459730 AT3G13550.1 48.276 145 75 0 2 146 37 181 2.36e-53 167
Msa0459730 AT1G16890.2 49.655 145 73 0 4 148 8 152 3.27e-48 153
Msa0459730 AT1G78870.2 48.966 145 74 0 4 148 8 152 2.97e-47 150
Msa0459730 AT5G62540.1 49.254 134 68 0 2 135 5 138 7.75e-46 147
Msa0459730 AT1G16890.3 47.403 154 72 1 4 148 8 161 1.50e-45 146
Msa0459730 AT1G14400.1 46.575 146 73 1 2 142 5 150 1.66e-45 146
Msa0459730 AT1G14400.2 46.575 146 73 1 2 142 5 150 1.66e-45 146
Msa0459730 AT2G02760.1 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0459730 AT2G02760.2 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0459730 AT2G02760.4 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0459730 AT2G02760.3 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0459730 AT1G36340.2 48.966 145 73 1 2 146 9 152 3.82e-45 145
Msa0459730 AT1G36340.1 48.966 145 73 1 2 146 9 152 3.82e-45 145
Msa0459730 AT3G13550.2 50.000 114 57 0 2 115 37 150 2.99e-42 138
Msa0459730 AT1G16890.1 52.101 119 57 0 30 148 1 119 4.40e-41 134
Msa0459730 AT1G78870.4 52.101 119 57 0 30 148 1 119 7.69e-41 133
Msa0459730 AT5G50870.1 45.833 144 76 2 5 146 6 149 1.88e-39 132
Msa0459730 AT1G78870.1 51.667 120 57 1 30 148 1 120 4.60e-39 128
Msa0459730 AT2G32790.1 49.342 152 68 4 2 148 30 177 2.34e-36 123
Msa0459730 AT5G50870.2 42.581 155 76 3 5 146 6 160 4.83e-36 123
Msa0459730 AT3G24515.1 44.586 157 74 3 3 146 8 164 2.26e-35 126
Msa0459730 AT3G24515.3 46.721 122 61 1 29 146 68 189 1.01e-32 120
Msa0459730 AT5G25760.1 38.776 147 86 3 5 147 7 153 1.11e-32 113
Msa0459730 AT5G25760.2 38.776 147 86 3 5 147 7 153 1.11e-32 113
Msa0459730 AT1G78870.3 48.571 105 54 0 4 108 8 112 2.15e-32 111
Msa0459730 AT3G20060.1 42.742 124 71 0 4 127 39 162 7.40e-31 109
Msa0459730 AT1G50490.1 43.548 124 70 0 4 127 38 161 2.22e-30 108
Msa0459730 AT3G20060.2 43.636 110 62 0 22 131 12 121 2.80e-29 104
Msa0459730 AT2G46030.5 36.486 148 91 3 1 146 1 147 2.64e-28 103
Msa0459730 AT2G46030.1 36.486 148 91 3 1 146 1 147 2.64e-28 103
Msa0459730 AT3G57870.1 34.868 152 92 2 1 145 5 156 4.56e-28 102
Msa0459730 AT2G46030.3 36.486 148 91 3 1 146 73 219 6.57e-28 103
Msa0459730 AT5G05080.2 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0459730 AT5G05080.3 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0459730 AT5G05080.1 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0459730 AT5G05080.4 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0459730 AT2G46030.4 39.024 123 73 2 26 146 28 150 9.73e-28 102
Msa0459730 AT2G46030.2 39.024 123 73 2 26 146 28 150 9.73e-28 102
Msa0459730 AT3G55380.1 36.567 134 71 2 15 134 19 152 8.26e-27 99.0
Msa0459730 AT5G41340.1 38.211 123 74 2 26 146 25 147 1.03e-26 99.4
Msa0459730 AT1G63800.2 38.211 123 74 2 26 146 10 132 1.22e-26 98.6
Msa0459730 AT1G63800.1 38.211 123 74 2 26 146 25 147 1.33e-26 99.0
Msa0459730 AT5G59300.1 34.722 144 80 2 15 144 18 161 2.36e-25 95.1
Msa0459730 AT4G36800.2 35.036 137 84 3 5 139 34 167 4.15e-25 95.1
Msa0459730 AT4G36800.1 35.036 137 84 3 5 139 34 167 4.15e-25 95.1
Msa0459730 AT3G46460.1 34.028 144 81 2 15 144 18 161 4.23e-25 94.7
Msa0459730 AT5G41340.2 40.385 104 60 2 45 146 9 112 1.42e-23 90.1
Msa0459730 AT2G18600.1 35.036 137 84 3 5 139 35 168 2.65e-23 90.5
Msa0459730 AT2G18600.2 35.036 137 84 3 5 139 35 168 2.65e-23 90.5
Msa0459730 AT3G17000.1 36.975 119 65 4 2 114 12 126 2.08e-20 85.5
Msa0459730 AT3G55380.2 28.994 169 72 3 15 135 19 187 1.33e-19 81.3
Msa0459730 AT1G75440.1 38.889 108 60 3 1 107 15 117 8.37e-19 78.2
Msa0459730 AT1G45050.1 37.864 103 62 2 1 102 15 116 2.80e-18 77.0
Msa0459730 AT5G42990.1 38.835 103 61 2 1 102 15 116 4.39e-18 76.3
Msa0459730 AT1G17280.9 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT1G17280.7 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT1G17280.6 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT1G17280.2 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT1G17280.5 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT1G17280.3 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT1G17280.1 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT1G17280.8 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0459730 AT4G36410.1 35.185 108 64 3 1 107 15 117 3.70e-17 73.9
Msa0459730 AT5G50430.1 34.188 117 68 3 4 115 8 120 4.45e-17 75.5
Msa0459730 AT5G50430.2 34.188 117 68 3 4 115 8 120 4.45e-17 75.5
Msa0459730 AT3G46460.3 30.631 111 64 1 47 144 9 119 1.19e-16 71.6
Msa0459730 AT3G46460.2 30.631 111 64 1 47 144 9 119 1.19e-16 71.6
Msa0459730 AT1G17280.4 31.707 123 69 3 4 115 8 126 8.56e-16 72.0
Msa0459730 AT3G15355.1 28.289 152 96 4 3 144 334 482 1.17e-11 61.6
Msa0459730 AT1G45050.2 34.831 89 56 2 1 88 15 102 1.27e-11 58.5
Msa0459730 AT1G53025.2 30.328 122 75 3 4 115 274 395 2.75e-11 60.5
Msa0459730 AT1G53025.1 30.328 122 75 3 4 115 274 395 3.07e-11 60.5
Msa0459730 AT1G53023.1 31.148 122 74 3 4 115 60 181 6.51e-11 59.3

Find 91 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAGAGAATGAGAGAAAA+AGG 0.204795 3_3:+56466257 None:intergenic
CAATACTGCAGGTTGCATTT+AGG 0.246319 3_3:-56464302 Msa0459730:intron
CTTCCATTGCTGTTGATGTT+TGG 0.284673 3_3:+56464264 None:intergenic
AAACGAAAGAAGGGATAATA+AGG 0.289702 3_3:+56466236 None:intergenic
TTGTACATATGTGCAATTTC+AGG 0.297625 3_3:+56463504 None:intergenic
TTCCATTGCTGTTGATGTTT+GGG 0.299216 3_3:+56464265 None:intergenic
ACACGGTCCATGCAATAAAA+TGG 0.325776 3_3:+56462913 None:intergenic
AATACCTTTGGTGGCTTGAA+TGG 0.326318 3_3:+56465548 None:intergenic
GTGAAAATTCTTAAGACATC+TGG 0.326730 3_3:-56463282 Msa0459730:three_prime_UTR
CACAAGCGGGTCATCGGGAT+TGG 0.353907 3_3:+56463527 None:intergenic
ATCTTCTCTGTTTGTCGTAA+TGG 0.356690 3_3:+56466314 None:intergenic
TATTTGTCTCGACATTCTTA+AGG 0.371764 3_3:-56464241 Msa0459730:CDS
GTATACCTACAAACATTATA+AGG 0.378355 3_3:-56462938 Msa0459730:three_prime_UTR
TGGCTCCTTATAATGTTTGT+AGG 0.385758 3_3:+56462933 None:intergenic
GTCAATCTGCTCATTGTTGA+CGG 0.386180 3_3:-56463553 Msa0459730:CDS
TTTAACAGCGAAGGAGGAAT+TGG 0.399882 3_3:+56463417 None:intergenic
GCGTGCGGTGGCTTCATACT+TGG 0.404055 3_3:+56463470 None:intergenic
GGTGGCTTGAATGGATAATC+AGG 0.410769 3_3:+56465557 None:intergenic
ATTAGGGTTGAAATTGAAAA+GGG 0.420801 3_3:+56466185 None:intergenic
TTCATTTATCATCGCTGCTT+GGG 0.423487 3_3:+56463228 None:intergenic
TGTCGTAATGGAATGTGTTT+GGG 0.427923 3_3:+56466326 None:intergenic
TTGTCGTAATGGAATGTGTT+TGG 0.428517 3_3:+56466325 None:intergenic
ACAAGCGGGTCATCGGGATT+GGG 0.433760 3_3:+56463528 None:intergenic
ACTAAATCAAACCTTGGAAA+TGG 0.438561 3_3:+56464197 None:intergenic
TCAGGCACAAGCGGGTCATC+GGG 0.450503 3_3:+56463522 None:intergenic
ATGAAGCCACCGCACGCAGC+TGG 0.456609 3_3:-56463464 Msa0459730:CDS
TAGGGTTTCAATACCTTTGG+TGG 0.458014 3_3:+56465539 None:intergenic
GATTAGGGTTGAAATTGAAA+AGG 0.459907 3_3:+56466184 None:intergenic
TTCAGGCACAAGCGGGTCAT+CGG 0.461878 3_3:+56463521 None:intergenic
TTTGTTTATATGTTATCTGC+TGG 0.463714 3_3:-56463139 Msa0459730:three_prime_UTR
TTTCATTTATCATCGCTGCT+TGG 0.470863 3_3:+56463227 None:intergenic
AACAAACATAATTCAATCTC+AGG 0.474332 3_3:+56463156 None:intergenic
TGGAACTCAACCATGGCAAT+GGG 0.477736 3_3:-56463321 Msa0459730:three_prime_UTR
GCAGGTTGCATTTAGGACTA+AGG 0.478226 3_3:-56464295 Msa0459730:intron
ACCTTGGAAATGGTCAGTGC+AGG 0.479512 3_3:+56464207 None:intergenic
TCATTGGCAAGCAACAATCA+TGG 0.479981 3_3:-56465636 Msa0459730:CDS
GTGACCTTACCTTACGCAAA+AGG 0.491996 3_3:-56466435 None:intergenic
TCTCCCCTTTGATTTGAGCA+TGG 0.493500 3_3:-56466117 Msa0459730:intron
TGGCTGAGGACATGTTTCAT+TGG 0.495052 3_3:-56465652 Msa0459730:CDS
CGATGCCATGCTCAAATCAA+AGG 0.498135 3_3:+56466112 None:intergenic
TGACCTGTACCAGCTTCAAC+TGG 0.505717 3_3:-56463036 Msa0459730:three_prime_UTR
CATTGCTGTTGATGTTTGGG+TGG 0.507557 3_3:+56464268 None:intergenic
CATTGGCAAGCAACAATCAT+GGG 0.510542 3_3:-56465635 Msa0459730:CDS
CCCTGCACTGACCATTTCCA+AGG 0.514922 3_3:-56464208 Msa0459730:intron
CCACCAACATCATGCAGTGC+AGG 0.515198 3_3:-56466050 Msa0459730:intron
ATGGAACTCAACCATGGCAA+TGG 0.520736 3_3:-56463322 Msa0459730:three_prime_UTR
CCTGCACTGCATGATGTTGG+TGG 0.521666 3_3:+56466050 None:intergenic
AAAACACACCACCAGCATAA+GGG 0.532990 3_3:+56465600 None:intergenic
ATTTGAGCATGGCATCGAAA+CGG 0.533369 3_3:-56466106 Msa0459730:exon
CTGTGTCCAGCTGCGTGCGG+TGG 0.538680 3_3:+56463458 None:intergenic
CACATATGTACAAGACTGAC+AGG 0.541007 3_3:-56463494 Msa0459730:CDS
GTACCAGTTGAAGCTGGTAC+AGG 0.546380 3_3:+56463033 None:intergenic
GATGCCATGCTCAAATCAAA+GGG 0.547382 3_3:+56466113 None:intergenic
CAACTAGAGAAAAGTGGTTG+AGG 0.553573 3_3:+56463069 None:intergenic
CCTGCTGATAGCCCTTATGC+TGG 0.566650 3_3:-56465611 Msa0459730:CDS
CCAGCATAAGGGCTATCAGC+AGG 0.567868 3_3:+56465611 None:intergenic
TTACATAAAACATAAATGCA+CGG 0.568066 3_3:+56463389 None:intergenic
TCACCTGCACTGCATGATGT+TGG 0.572393 3_3:+56466047 None:intergenic
CACCCAAACATCAACAGCAA+TGG 0.573454 3_3:-56464267 Msa0459730:CDS
TAAGCTATAGACACATGAGT+GGG 0.576825 3_3:+56462996 None:intergenic
TGAAACATGTCCTCAGCCAC+AGG 0.579671 3_3:+56465656 None:intergenic
GTGCAATTTCAGGCACAAGC+GGG 0.582074 3_3:+56463514 None:intergenic
AAGACTGTACCAGTTGAAGC+TGG 0.584229 3_3:+56463027 None:intergenic
CCTTGGAAATGGTCAGTGCA+GGG 0.590131 3_3:+56464208 None:intergenic
ATAATCAATTCCCATTGCCA+TGG 0.591438 3_3:+56463311 None:intergenic
CTGGACACAGAAGTATGCCA+TGG 0.597546 3_3:-56463445 Msa0459730:CDS
AGCTGGTACAGGTCAACAGA+TGG 0.600544 3_3:+56463044 None:intergenic
GCAAATCCTTGAGCTCCTTG+AGG 0.601494 3_3:+56466081 None:intergenic
ACGGTTGCATTTAACAGCGA+AGG 0.603135 3_3:+56463408 None:intergenic
GTTGCATTTAACAGCGAAGG+AGG 0.604567 3_3:+56463411 None:intergenic
GGGTTTGATTGAAATTGAAG+AGG 0.605266 3_3:+56466205 None:intergenic
ACGGATCCTCAAGGAGCTCA+AGG 0.606874 3_3:-56466087 Msa0459730:CDS
AGGAGGAATTGGTCATCCCA+TGG 0.622867 3_3:+56463428 None:intergenic
TCGACATTCTTAAGGAACAA+TGG 0.626316 3_3:-56464233 Msa0459730:CDS
GGAGCTCAAGGATTTGCAGA+AGG 0.628349 3_3:-56466075 Msa0459730:CDS
GCTGATAGCCCTTATGCTGG+TGG 0.629848 3_3:-56465608 Msa0459730:CDS
ATGGACCAACTAGAGAAAAG+TGG 0.632346 3_3:+56463063 None:intergenic
TTTGTTATTTATTGTCAACA+TGG 0.637302 3_3:-56463341 Msa0459730:three_prime_UTR
GAAAATTGTAGTTACGAACA+TGG 0.643887 3_3:-56463208 Msa0459730:three_prime_UTR
GGCATCGAAACGGATCCTCA+AGG 0.650007 3_3:-56466096 Msa0459730:CDS
TGGACACAGAAGTATGCCAT+GGG 0.652544 3_3:-56463444 Msa0459730:CDS
TGTGCAATTTCAGGCACAAG+CGG 0.661456 3_3:+56463513 None:intergenic
GGCTTGAATGGATAATCAGG+AGG 0.662651 3_3:+56465560 None:intergenic
AGGAACAATGAAACATCCAC+AGG 0.663163 3_3:+56463176 None:intergenic
AACATGGTGATTATTGCCTG+TGG 0.665031 3_3:-56463192 Msa0459730:three_prime_UTR
TTATCCATTCAAGCCACCAA+AGG 0.668003 3_3:-56465552 Msa0459730:intron
AAGCTATAGACACATGAGTG+GGG 0.683606 3_3:+56462997 None:intergenic
GTCAACATGGAACTCAACCA+TGG 0.686063 3_3:-56463328 Msa0459730:three_prime_UTR
CTTCTGTGTCCAGCTGCGTG+CGG 0.688818 3_3:+56463455 None:intergenic
TTAAGCTATAGACACATGAG+TGG 0.689052 3_3:+56462995 None:intergenic
ATGCCATGCTCAAATCAAAG+GGG 0.742920 3_3:+56466114 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATGATAAAAGAAAAAAA+GGG + chr3_3:56463340-56463359 None:intergenic 10.0%
!! TAAATGCTAATTATTATATT+TGG - chr3_3:56464207-56464226 Msa0459730:intron 10.0%
!! GATAATTAATGATTTATATA+AGG - chr3_3:56465171-56465190 Msa0459730:intron 10.0%
!! CTATATGATAAAAGAAAAAA+AGG + chr3_3:56463341-56463360 None:intergenic 15.0%
!! AAAACAATAAAACAACAAAA+GGG + chr3_3:56463457-56463476 None:intergenic 15.0%
!! AAAAACAATAAAACAACAAA+AGG + chr3_3:56463458-56463477 None:intergenic 15.0%
!!! TTTTTATCAATGATTTGTTA+TGG - chr3_3:56463473-56463492 Msa0459730:CDS 15.0%
!! AACATATCTAACAAATAATT+TGG + chr3_3:56463565-56463584 None:intergenic 15.0%
!!! ATTATTTGTTAGATATGTTA+TGG - chr3_3:56463566-56463585 Msa0459730:intron 15.0%
!!! TTTGAAAGTTTTATTCTTTT+TGG - chr3_3:56463908-56463927 Msa0459730:intron 15.0%
!! TGAAAACAGAAAAAATAATA+TGG + chr3_3:56464075-56464094 None:intergenic 15.0%
!! TAGTATAAATAAGAAATTGT+AGG + chr3_3:56464392-56464411 None:intergenic 15.0%
!!! TATTTTTTTCAATTAACACA+GGG + chr3_3:56464593-56464612 None:intergenic 15.0%
!!! TTATTTTTTTCAATTAACAC+AGG + chr3_3:56464594-56464613 None:intergenic 15.0%
!! TTGAAAAAAATAATGATCAT+TGG - chr3_3:56464601-56464620 Msa0459730:intron 15.0%
!!! ATAATTTGTTGAGATTTGTT+TGG - chr3_3:56463849-56463868 Msa0459730:intron 20.0%
!!! ATTTTTAAAAAATCGCTATG+AGG - chr3_3:56464106-56464125 Msa0459730:intron 20.0%
!! AAAAAATAATGATCATTGGT+TGG - chr3_3:56464605-56464624 Msa0459730:intron 20.0%
!! TTTGTTAATTGTTTGGAAAA+TGG - chr3_3:56465210-56465229 Msa0459730:intron 20.0%
!!! AAATCATGAATCATTTTCTT+GGG + chr3_3:56465432-56465451 None:intergenic 20.0%
!! TTAAATTCCTATCAATCATA+TGG + chr3_3:56465627-56465646 None:intergenic 20.0%
!! TTACATAAAACATAAATGCA+CGG + chr3_3:56465948-56465967 None:intergenic 20.0%
!!! TTTGTTATTTATTGTCAACA+TGG - chr3_3:56465993-56466012 Msa0459730:intron 20.0%
! ATTAGGGTTGAAATTGAAAA+GGG + chr3_3:56463152-56463171 None:intergenic 25.0%
!! TTATGGATCTGTTATTTGAA+GGG - chr3_3:56463490-56463509 Msa0459730:CDS 25.0%
! GATTGTTTACATGAAACTTT+AGG - chr3_3:56463536-56463555 Msa0459730:CDS 25.0%
!!! ATGATGATGTTTTGTGTTTT+AGG - chr3_3:56463654-56463673 Msa0459730:intron 25.0%
! TTGAGATAATTAAAGAGCAA+GGG + chr3_3:56464429-56464448 None:intergenic 25.0%
! TACACTTTAATCAATGAATC+AGG - chr3_3:56465237-56465256 Msa0459730:intron 25.0%
!! CAAATCATGAATCATTTTCT+TGG + chr3_3:56465433-56465452 None:intergenic 25.0%
!!! AGAAGAACTGTGATTTTTTT+TGG - chr3_3:56465478-56465497 Msa0459730:intron 25.0%
! AACAAACATAATTCAATCTC+AGG + chr3_3:56466181-56466200 None:intergenic 25.0%
! TTTGTTTATATGTTATCTGC+TGG - chr3_3:56466195-56466214 Msa0459730:five_prime_UTR 25.0%
! GTATACCTACAAACATTATA+AGG - chr3_3:56466396-56466415 Msa0459730:five_prime_UTR 25.0%
!!! AGGTATTTTTAAAGCGTGTT+TGG - chr3_3:56462919-56462938 Msa0459730:three_prime_UTR 30.0%
! CTAAATTACGCGTTTTTGAA+TGG + chr3_3:56462972-56462991 None:intergenic 30.0%
AAACGAAAGAAGGGATAATA+AGG + chr3_3:56463101-56463120 None:intergenic 30.0%
GAAGAAGAAAAACGAAAGAA+GGG + chr3_3:56463110-56463129 None:intergenic 30.0%
GATTAGGGTTGAAATTGAAA+AGG + chr3_3:56463153-56463172 None:intergenic 30.0%
! GTTATGGATCTGTTATTTGA+AGG - chr3_3:56463489-56463508 Msa0459730:CDS 30.0%
!! TGTTATTTGAAGGGTTTTTG+AGG - chr3_3:56463499-56463518 Msa0459730:CDS 30.0%
!!! TTTTGAGGTGATTTGGATTT+AGG - chr3_3:56463514-56463533 Msa0459730:CDS 30.0%
ATGTTATGGTTTATGAAGCT+AGG - chr3_3:56463580-56463599 Msa0459730:intron 30.0%
AAAAATAATATGGTCCCTGT+CGG + chr3_3:56464065-56464084 None:intergenic 30.0%
TTTAAAAAATCGCTATGAGG+AGG - chr3_3:56464109-56464128 Msa0459730:intron 30.0%
! AGCTTGATCGATAATTTTGA+CGG + chr3_3:56464235-56464254 None:intergenic 30.0%
ACTATTATCAAGTCCTTACT+TGG + chr3_3:56464356-56464375 None:intergenic 30.0%
ATAATTAAAGAGCAAGGGAA+TGG + chr3_3:56464424-56464443 None:intergenic 30.0%
CTTGAGATAATTAAAGAGCA+AGG + chr3_3:56464430-56464449 None:intergenic 30.0%
AGATCAGTGGAAAACAAAAA+AGG + chr3_3:56464654-56464673 None:intergenic 30.0%
GTCAATATTAACAACATCCA+CGG + chr3_3:56464835-56464854 None:intergenic 30.0%
AACATAAAACTATGTTCAGG+TGG + chr3_3:56464864-56464883 None:intergenic 30.0%
CCTAACATAAAACTATGTTC+AGG + chr3_3:56464867-56464886 None:intergenic 30.0%
!!! CCTGAACATAGTTTTATGTT+AGG - chr3_3:56464864-56464883 Msa0459730:intron 30.0%
! TTTTCTTTTTCTTGCTGATG+AGG - chr3_3:56464891-56464910 Msa0459730:intron 30.0%
GTTTGCAAACTCAATTAAAC+CGG + chr3_3:56464957-56464976 None:intergenic 30.0%
TTTGCAAACATCATGTAGTT+TGG - chr3_3:56464968-56464987 Msa0459730:intron 30.0%
TATTTGTCTCGACATTCTTA+AGG - chr3_3:56465093-56465112 Msa0459730:intron 30.0%
ACTAAATCAAACCTTGGAAA+TGG + chr3_3:56465140-56465159 None:intergenic 30.0%
ACAACAACTAAATCAAACCT+TGG + chr3_3:56465146-56465165 None:intergenic 30.0%
!! GACAAGCTTTGTTAATTGTT+TGG - chr3_3:56465203-56465222 Msa0459730:intron 30.0%
! TTTTGTTGATGTCATTCTTG+TGG - chr3_3:56465370-56465389 Msa0459730:intron 30.0%
TTTGTTGATGTCATTCTTGT+GGG - chr3_3:56465371-56465390 Msa0459730:intron 30.0%
AAGTTGACCATATGATTGAT+AGG - chr3_3:56465617-56465636 Msa0459730:CDS 30.0%
! ATCCTAACAGTCACTATTTT+AGG - chr3_3:56465751-56465770 Msa0459730:intron 30.0%
TTGTACATATGTGCAATTTC+AGG + chr3_3:56465833-56465852 None:intergenic 30.0%
GTGAAAATTCTTAAGACATC+TGG - chr3_3:56466052-56466071 Msa0459730:CDS 30.0%
GAAAATTGTAGTTACGAACA+TGG - chr3_3:56466126-56466145 Msa0459730:intron 30.0%
!! AGGTTGGTAAACAACAAAAA+TGG + chr3_3:56466236-56466255 None:intergenic 30.0%
AATCAACCAAAAACAAGACT+AGG + chr3_3:56466375-56466394 None:intergenic 30.0%
! TGTCGTAATGGAATGTGTTT+GGG + chr3_3:56463011-56463030 None:intergenic 35.0%
! TTGTCGTAATGGAATGTGTT+TGG + chr3_3:56463012-56463031 None:intergenic 35.0%
ATCTTCTCTGTTTGTCGTAA+TGG + chr3_3:56463023-56463042 None:intergenic 35.0%
GAGAGAGAATGAGAGAAAAA+GGG + chr3_3:56463079-56463098 None:intergenic 35.0%
GGAAGAAGAAAAACGAAAGA+AGG + chr3_3:56463111-56463130 None:intergenic 35.0%
GGGTTTGATTGAAATTGAAG+AGG + chr3_3:56463132-56463151 None:intergenic 35.0%
CAGATCATACATACAAGCTA+GGG + chr3_3:56463816-56463835 None:intergenic 35.0%
ACAGATCATACATACAAGCT+AGG + chr3_3:56463817-56463836 None:intergenic 35.0%
ATTATACACACACACAAGCA+CGG + chr3_3:56463934-56463953 None:intergenic 35.0%
AAAATAATATGGTCCCTGTC+GGG + chr3_3:56464064-56464083 None:intergenic 35.0%
!! GCTTGATCGATAATTTTGAC+GGG + chr3_3:56464234-56464253 None:intergenic 35.0%
!! AAGCAAACTTTTTCTGAAGG+CGG + chr3_3:56464258-56464277 None:intergenic 35.0%
!! GAGAAGCAAACTTTTTCTGA+AGG + chr3_3:56464261-56464280 None:intergenic 35.0%
TATTTGTGTTGCAACTTGCA+AGG - chr3_3:56464503-56464522 Msa0459730:intron 35.0%
TTATTGTCTTGCAATACTGC+AGG - chr3_3:56465021-56465040 Msa0459730:intron 35.0%
! TTCCATTGCTGTTGATGTTT+GGG + chr3_3:56465072-56465091 None:intergenic 35.0%
TCGACATTCTTAAGGAACAA+TGG - chr3_3:56465101-56465120 Msa0459730:intron 35.0%
AACAACAACATTCCAAATGC+AGG + chr3_3:56465691-56465710 None:intergenic 35.0%
! GTTGTTGTTCTTATGTGTCA+TGG - chr3_3:56465702-56465721 Msa0459730:intron 35.0%
CACCTAAAATAGTGACTGTT+AGG + chr3_3:56465756-56465775 None:intergenic 35.0%
ATAATCAATTCCCATTGCCA+TGG + chr3_3:56466026-56466045 None:intergenic 35.0%
TTCATTTATCATCGCTGCTT+GGG + chr3_3:56466109-56466128 None:intergenic 35.0%
TTTCATTTATCATCGCTGCT+TGG + chr3_3:56466110-56466129 None:intergenic 35.0%
!!! GTTGAGGTAGATTTTTAGGT+TGG + chr3_3:56466252-56466271 None:intergenic 35.0%
! AGTGGTTGAGGTAGATTTTT+AGG + chr3_3:56466256-56466275 None:intergenic 35.0%
TAAGCTATAGACACATGAGT+GGG + chr3_3:56466341-56466360 None:intergenic 35.0%
TTAAGCTATAGACACATGAG+TGG + chr3_3:56466342-56466361 None:intergenic 35.0%
TGGCTCCTTATAATGTTTGT+AGG + chr3_3:56466404-56466423 None:intergenic 35.0%
!! TAAGGAGCCATTTTATTGCA+TGG - chr3_3:56466414-56466433 Msa0459730:five_prime_UTR 35.0%
AGAATGAGAGAAAAAGGGAC+AGG + chr3_3:56463074-56463093 None:intergenic 40.0%
GGAGAGAGAATGAGAGAAAA+AGG + chr3_3:56463080-56463099 None:intergenic 40.0%
AGAGAGAGAGAGAGAGATTA+GGG + chr3_3:56463168-56463187 None:intergenic 40.0%
ATGCCATGCTCAAATCAAAG+GGG + chr3_3:56463223-56463242 None:intergenic 40.0%
GATGCCATGCTCAAATCAAA+GGG + chr3_3:56463224-56463243 None:intergenic 40.0%
! ATTTGAGCATGGCATCGAAA+CGG - chr3_3:56463228-56463247 Msa0459730:three_prime_UTR 40.0%
!!! GAAGGGTTTTTGAGGTGATT+TGG - chr3_3:56463507-56463526 Msa0459730:CDS 40.0%
!! GTTTTGTGTTTTAGGTCCTG+TGG - chr3_3:56463662-56463681 Msa0459730:intron 40.0%
TCATTGGCAAGCAACAATCA+TGG - chr3_3:56463698-56463717 Msa0459730:intron 40.0%
CATTGGCAAGCAACAATCAT+GGG - chr3_3:56463699-56463718 Msa0459730:intron 40.0%
AAAACACACCACCAGCATAA+GGG + chr3_3:56463737-56463756 None:intergenic 40.0%
AAAAACACACCACCAGCATA+AGG + chr3_3:56463738-56463757 None:intergenic 40.0%
!! TTATGCTGGTGGTGTGTTTT+TGG - chr3_3:56463737-56463756 Msa0459730:intron 40.0%
TTATCCATTCAAGCCACCAA+AGG - chr3_3:56463782-56463801 Msa0459730:intron 40.0%
! AATACCTTTGGTGGCTTGAA+TGG + chr3_3:56463789-56463808 None:intergenic 40.0%
TAGGGTTTCAATACCTTTGG+TGG + chr3_3:56463798-56463817 None:intergenic 40.0%
AGCTAGGGTTTCAATACCTT+TGG + chr3_3:56463801-56463820 None:intergenic 40.0%
ATCAAATCTATCTAGCCGTG+CGG - chr3_3:56463993-56464012 Msa0459730:intron 40.0%
!!! TGTTTTGAGGGGTGAATGTT+TGG - chr3_3:56464026-56464045 Msa0459730:intron 40.0%
GCAATGAAAGCTTTGCATCT+TGG + chr3_3:56464183-56464202 None:intergenic 40.0%
ATCTCAAGCAAACAAGCTAG+TGG - chr3_3:56464442-56464461 Msa0459730:intron 40.0%
TCTCAAGCAAACAAGCTAGT+GGG - chr3_3:56464443-56464462 Msa0459730:intron 40.0%
TCTTGAGGCAGATTTATGCT+AGG + chr3_3:56464473-56464492 None:intergenic 40.0%
CAACAGTTTGCAATACACTC+AGG + chr3_3:56464562-56464581 None:intergenic 40.0%
AACTGGGACAAAAAGATCAG+TGG + chr3_3:56464667-56464686 None:intergenic 40.0%
TCTCAAACCAATGAAATCCG+TGG - chr3_3:56464815-56464834 Msa0459730:intron 40.0%
ACAACATCCACGGATTTCAT+TGG + chr3_3:56464825-56464844 None:intergenic 40.0%
CCAAGTACAGTATACTTGTC+CGG - chr3_3:56464935-56464954 Msa0459730:intron 40.0%
CAATACTGCAGGTTGCATTT+AGG - chr3_3:56465032-56465051 Msa0459730:intron 40.0%
! CTTCCATTGCTGTTGATGTT+TGG + chr3_3:56465073-56465092 None:intergenic 40.0%
TTGATGTCATTCTTGTGGGA+AGG - chr3_3:56465375-56465394 Msa0459730:intron 40.0%
ACACACGCAAACTATATCTC+AGG + chr3_3:56465588-56465607 None:intergenic 40.0%
CAAATGCAGGTCGTTAAACA+CGG + chr3_3:56465678-56465697 None:intergenic 40.0%
GTCAATCTGCTCATTGTTGA+CGG - chr3_3:56465781-56465800 Msa0459730:intron 40.0%
CACATATGTACAAGACTGAC+AGG - chr3_3:56465840-56465859 Msa0459730:intron 40.0%
TTTAACAGCGAAGGAGGAAT+TGG + chr3_3:56465920-56465939 None:intergenic 40.0%
! GACATCTGGTGCAAATTTTC+TGG - chr3_3:56466066-56466085 Msa0459730:CDS 40.0%
AACATGGTGATTATTGCCTG+TGG - chr3_3:56466142-56466161 Msa0459730:intron 40.0%
AGGAACAATGAAACATCCAC+AGG + chr3_3:56466161-56466180 None:intergenic 40.0%
CAACTAGAGAAAAGTGGTTG+AGG + chr3_3:56466268-56466287 None:intergenic 40.0%
!! CTCAACCACTTTTCTCTAGT+TGG - chr3_3:56466266-56466285 Msa0459730:five_prime_UTR 40.0%
ATGGACCAACTAGAGAAAAG+TGG + chr3_3:56466274-56466293 None:intergenic 40.0%
AAGCTATAGACACATGAGTG+GGG + chr3_3:56466340-56466359 None:intergenic 40.0%
!!! CTCTGTCCTAGTCTTGTTTT+TGG - chr3_3:56466366-56466385 Msa0459730:five_prime_UTR 40.0%
GAGAGAGAGAGAGAGAGATT+AGG + chr3_3:56463169-56463188 None:intergenic 45.0%
!! TCTCCCCTTTGATTTGAGCA+TGG - chr3_3:56463217-56463236 Msa0459730:three_prime_UTR 45.0%
CGATGCCATGCTCAAATCAA+AGG + chr3_3:56463225-56463244 None:intergenic 45.0%
TGGCTGAGGACATGTTTCAT+TGG - chr3_3:56463682-56463701 Msa0459730:intron 45.0%
GGCTTGAATGGATAATCAGG+AGG + chr3_3:56463777-56463796 None:intergenic 45.0%
GGTGGCTTGAATGGATAATC+AGG + chr3_3:56463780-56463799 None:intergenic 45.0%
!! CTCGCAGAGGATCGATTTTA+TGG + chr3_3:56464327-56464346 None:intergenic 45.0%
CATAAAATCGATCCTCTGCG+AGG - chr3_3:56464325-56464344 Msa0459730:intron 45.0%
CTCAAGCAAACAAGCTAGTG+GGG - chr3_3:56464444-56464463 Msa0459730:intron 45.0%
AAATAGCCTGAGGCTTCTTG+AGG + chr3_3:56464488-56464507 None:intergenic 45.0%
GTTGCAACACAAATAGCCTG+AGG + chr3_3:56464498-56464517 None:intergenic 45.0%
TTGCAATACACTCAGGCTCT+CGG + chr3_3:56464555-56464574 None:intergenic 45.0%
ACATGCATGCAAGCGAAACT+GGG + chr3_3:56464683-56464702 None:intergenic 45.0%
AGACGACAATTGTGAACGTG+TGG + chr3_3:56464712-56464731 None:intergenic 45.0%
CCGGACAAGTATACTGTACT+TGG + chr3_3:56464938-56464957 None:intergenic 45.0%
! GCAGGTTGCATTTAGGACTA+AGG - chr3_3:56465039-56465058 Msa0459730:intron 45.0%
! CATTGCTGTTGATGTTTGGG+TGG + chr3_3:56465069-56465088 None:intergenic 45.0%
CACCCAAACATCAACAGCAA+TGG - chr3_3:56465067-56465086 Msa0459730:intron 45.0%
CGTGTTTAACGACCTGCATT+TGG - chr3_3:56465676-56465695 Msa0459730:intron 45.0%
TGTGCAATTTCAGGCACAAG+CGG + chr3_3:56465824-56465843 None:intergenic 45.0%
! TGGACACAGAAGTATGCCAT+GGG - chr3_3:56465890-56465909 Msa0459730:intron 45.0%
GTTGCATTTAACAGCGAAGG+AGG + chr3_3:56465926-56465945 None:intergenic 45.0%
ACGGTTGCATTTAACAGCGA+AGG + chr3_3:56465929-56465948 None:intergenic 45.0%
GTCAACATGGAACTCAACCA+TGG - chr3_3:56466006-56466025 Msa0459730:intron 45.0%
ATGGAACTCAACCATGGCAA+TGG - chr3_3:56466012-56466031 Msa0459730:intron 45.0%
TGGAACTCAACCATGGCAAT+GGG - chr3_3:56466013-56466032 Msa0459730:intron 45.0%
AAGACTGTACCAGTTGAAGC+TGG + chr3_3:56466310-56466329 None:intergenic 45.0%
!!! ATGATTTATTTTTTAAATTA+TGG - chr3_3:56463428-56463447 Msa0459730:exon 5.0%
!!! TGATTTATTTTTTAAATTAT+GGG - chr3_3:56463429-56463448 Msa0459730:exon 5.0%
GAGAGAAAAAGGGACAGGCA+AGG + chr3_3:56463069-56463088 None:intergenic 50.0%
TCAAAGGGGAGAGAGAGAGA+GGG + chr3_3:56463209-56463228 None:intergenic 50.0%
ATCAAAGGGGAGAGAGAGAG+AGG + chr3_3:56463210-56463229 None:intergenic 50.0%
GCAAATCCTTGAGCTCCTTG+AGG + chr3_3:56463256-56463275 None:intergenic 50.0%
GGAGCTCAAGGATTTGCAGA+AGG - chr3_3:56463259-56463278 Msa0459730:three_prime_UTR 50.0%
TCACCTGCACTGCATGATGT+TGG + chr3_3:56463290-56463309 None:intergenic 50.0%
!! TGTTTTAGGTCCTGTGGCTG+AGG - chr3_3:56463668-56463687 Msa0459730:intron 50.0%
TGAAACATGTCCTCAGCCAC+AGG + chr3_3:56463681-56463700 None:intergenic 50.0%
AAATCTATCTAGCCGTGCGG+TGG - chr3_3:56463996-56464015 Msa0459730:intron 50.0%
TTGAGGGGTGAATGTTTGGC+AGG - chr3_3:56464030-56464049 Msa0459730:intron 50.0%
CTATGAGGAGGTTCGAACCA+TGG - chr3_3:56464121-56464140 Msa0459730:intron 50.0%
GTCGTGGTATTACAGCGCAT+CGG + chr3_3:56464159-56464178 None:intergenic 50.0%
GAGCAAGGGAATGGACGAAT+GGG + chr3_3:56464415-56464434 None:intergenic 50.0%
AGAGCAAGGGAATGGACGAA+TGG + chr3_3:56464416-56464435 None:intergenic 50.0%
AATCTGCCTCAAGAAGCCTC+AGG - chr3_3:56464479-56464498 Msa0459730:intron 50.0%
ACCTTACCTCAATGCCGAAG+TGG - chr3_3:56464529-56464548 Msa0459730:intron 50.0%
CACATGCATGCAAGCGAAAC+TGG + chr3_3:56464684-56464703 None:intergenic 50.0%
CCTTGGAAATGGTCAGTGCA+GGG + chr3_3:56465129-56465148 None:intergenic 50.0%
ACCTTGGAAATGGTCAGTGC+AGG + chr3_3:56465130-56465149 None:intergenic 50.0%
GTGCAATTTCAGGCACAAGC+GGG + chr3_3:56465823-56465842 None:intergenic 50.0%
! CTGGACACAGAAGTATGCCA+TGG - chr3_3:56465889-56465908 Msa0459730:intron 50.0%
AGGAGGAATTGGTCATCCCA+TGG + chr3_3:56465909-56465928 None:intergenic 50.0%
AGCTGGTACAGGTCAACAGA+TGG + chr3_3:56466293-56466312 None:intergenic 50.0%
TGACCTGTACCAGCTTCAAC+TGG - chr3_3:56466298-56466317 Msa0459730:five_prime_UTR 50.0%
GTACCAGTTGAAGCTGGTAC+AGG + chr3_3:56466304-56466323 None:intergenic 50.0%
! GGCATCGAAACGGATCCTCA+AGG - chr3_3:56463238-56463257 Msa0459730:three_prime_UTR 55.0%
ACGGATCCTCAAGGAGCTCA+AGG - chr3_3:56463247-56463266 Msa0459730:three_prime_UTR 55.0%
CCTGCACTGCATGATGTTGG+TGG + chr3_3:56463287-56463306 None:intergenic 55.0%
CCACCAACATCATGCAGTGC+AGG - chr3_3:56463284-56463303 Msa0459730:three_prime_UTR 55.0%
CCAGCATAAGGGCTATCAGC+AGG + chr3_3:56463726-56463745 None:intergenic 55.0%
CCTGCTGATAGCCCTTATGC+TGG - chr3_3:56463723-56463742 Msa0459730:intron 55.0%
GCTGATAGCCCTTATGCTGG+TGG - chr3_3:56463726-56463745 Msa0459730:intron 55.0%
! ATCGGTTTACCTCGCGTCCA+TGG + chr3_3:56464141-56464160 None:intergenic 55.0%
AGCTTTGCATCTTGGCGTCG+TGG + chr3_3:56464175-56464194 None:intergenic 55.0%
TACTTGGTACGACCTCGCAG+AGG + chr3_3:56464340-56464359 None:intergenic 55.0%
CTGCGAGGTCGTACCAAGTA+AGG - chr3_3:56464340-56464359 Msa0459730:intron 55.0%
! GCCACTTCGGCATTGAGGTA+AGG + chr3_3:56464533-56464552 None:intergenic 55.0%
! CCCTGCACTGACCATTTCCA+AGG - chr3_3:56465126-56465145 Msa0459730:intron 55.0%
ACAAGCGGGTCATCGGGATT+GGG + chr3_3:56465809-56465828 None:intergenic 55.0%
TTCAGGCACAAGCGGGTCAT+CGG + chr3_3:56465816-56465835 None:intergenic 55.0%
TCTATCTAGCCGTGCGGTGG+CGG - chr3_3:56463999-56464018 Msa0459730:intron 60.0%
!!! GGTGGCGGGGTCTGTTTTGA+GGG - chr3_3:56464014-56464033 Msa0459730:intron 60.0%
!! GTGGCGGGGTCTGTTTTGAG+GGG - chr3_3:56464015-56464034 Msa0459730:intron 60.0%
AGGTTCGAACCATGGACGCG+AGG - chr3_3:56464129-56464148 Msa0459730:intron 60.0%
TCTCGGCCACTTCGGCATTG+AGG + chr3_3:56464538-56464557 None:intergenic 60.0%
ACTCAGGCTCTCGGCCACTT+CGG + chr3_3:56464546-56464565 None:intergenic 60.0%
CACAAGCGGGTCATCGGGAT+TGG + chr3_3:56465810-56465829 None:intergenic 60.0%
TCAGGCACAAGCGGGTCATC+GGG + chr3_3:56465815-56465834 None:intergenic 60.0%
GCGTGCGGTGGCTTCATACT+TGG + chr3_3:56465867-56465886 None:intergenic 60.0%
CTTCTGTGTCCAGCTGCGTG+CGG + chr3_3:56465882-56465901 None:intergenic 60.0%
CTATCTAGCCGTGCGGTGGC+GGG - chr3_3:56464000-56464019 Msa0459730:intron 65.0%
TATCTAGCCGTGCGGTGGCG+GGG - chr3_3:56464001-56464020 Msa0459730:intron 65.0%
AAACAGACCCCGCCACCGCA+CGG + chr3_3:56464011-56464030 None:intergenic 65.0%
!!! CGGTGGCGGGGTCTGTTTTG+AGG - chr3_3:56464013-56464032 Msa0459730:intron 65.0%
GCAGGCAGTCGATCCCGACA+GGG - chr3_3:56464048-56464067 Msa0459730:intron 65.0%
ATGAAGCCACCGCACGCAGC+TGG - chr3_3:56465870-56465889 Msa0459730:intron 65.0%
GGCAGGCAGTCGATCCCGAC+AGG - chr3_3:56464047-56464066 Msa0459730:intron 70.0%
CTGTGTCCAGCTGCGTGCGG+TGG + chr3_3:56465879-56465898 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 56462914 56466442 56462914 ID=Msa0459730;Name=Msa0459730
chr3_3 mRNA 56462914 56466442 56462914 ID=Msa0459730-mRNA-1;Parent=Msa0459730;Name=Msa0459730-mRNA-1;_AED=0.09;_eAED=0.09;_QI=288|1|1|1|0.75|0.6|5|526|148
chr3_3 exon 56462914 56463583 56462914 ID=Msa0459730-mRNA-1:exon:11303;Parent=Msa0459730-mRNA-1
chr3_3 exon 56464209 56464313 56464209 ID=Msa0459730-mRNA-1:exon:11302;Parent=Msa0459730-mRNA-1
chr3_3 exon 56465553 56465680 56465553 ID=Msa0459730-mRNA-1:exon:11301;Parent=Msa0459730-mRNA-1
chr3_3 exon 56466051 56466134 56466051 ID=Msa0459730-mRNA-1:exon:11300;Parent=Msa0459730-mRNA-1
chr3_3 exon 56466169 56466442 56466169 ID=Msa0459730-mRNA-1:exon:11299;Parent=Msa0459730-mRNA-1
chr3_3 five_prime_UTR 56466169 56466442 56466169 ID=Msa0459730-mRNA-1:five_prime_utr;Parent=Msa0459730-mRNA-1
chr3_3 five_prime_UTR 56466121 56466134 56466121 ID=Msa0459730-mRNA-1:five_prime_utr;Parent=Msa0459730-mRNA-1
chr3_3 CDS 56466051 56466120 56466051 ID=Msa0459730-mRNA-1:cds;Parent=Msa0459730-mRNA-1
chr3_3 CDS 56465553 56465680 56465553 ID=Msa0459730-mRNA-1:cds;Parent=Msa0459730-mRNA-1
chr3_3 CDS 56464209 56464313 56464209 ID=Msa0459730-mRNA-1:cds;Parent=Msa0459730-mRNA-1
chr3_3 CDS 56463440 56463583 56463440 ID=Msa0459730-mRNA-1:cds;Parent=Msa0459730-mRNA-1
chr3_3 three_prime_UTR 56462914 56463439 56462914 ID=Msa0459730-mRNA-1:three_prime_utr;Parent=Msa0459730-mRNA-1
Gene Sequence

>Msa0459730

ATGGCATCGAAACGGATCCTCAAGGAGCTCAAGGATTTGCAGAAGGATCCACCAACATCATGCAGTGCAGGTCCTGTGGCTGAGGACATGTTTCATTGGCAAGCAACAATCATGGGACCTGCTGATAGCCCTTATGCTGGTGGTGTGTTTTTGGTTTCAATTCATTTTCCTCCTGATTATCCATTCAAGCCACCAAAGGTTGCATTTAGGACTAAGGTATTCCACCCAAACATCAACAGCAATGGAAGTATTTGTCTCGACATTCTTAAGGAACAATGGAGCCCTGCACTGACCATTTCCAAGGTGCTTTTGTCAATCTGCTCATTGTTGACGGATCCCAATCCCGATGACCCGCTTGTGCCTGAAATTGCACATATGTACAAGACTGACAGGACCAAGTATGAAGCCACCGCACGCAGCTGGACACAGAAGTATGCCATGGGATGA
Protein sequence

>Msa0459730

MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRTKYEATARSWTQKYAMG*