AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0506530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0506530 MtrunA17_Chr3g0108261 100.000 148 0 0 1 148 1 148 5.28e-110 308
Msa0506530 MtrunA17_Chr5g0432831 98.649 148 2 0 1 148 1 148 1.15e-108 305
Msa0506530 MtrunA17_Chr7g0275361 97.297 148 4 0 1 148 1 148 2.12e-107 302
Msa0506530 MtrunA17_Chr8g0369311 93.919 148 9 0 1 148 57 204 1.02e-103 295
Msa0506530 MtrunA17_Chr1g0154011 91.892 148 12 0 1 148 1 148 4.66e-103 291
Msa0506530 MtrunA17_Chr4g0037011 91.892 148 12 0 1 148 1 148 5.03e-103 291
Msa0506530 MtrunA17_Chr8g0378601 51.034 145 71 0 2 146 23 167 5.60e-55 170
Msa0506530 MtrunA17_Chr2g0316201 48.966 145 74 0 4 148 8 152 1.14e-47 151
Msa0506530 MtrunA17_Chr2g0316211 48.611 144 74 0 5 148 9 152 1.22e-46 148
Msa0506530 MtrunA17_Chr4g0060351 45.890 146 74 1 2 142 5 150 3.03e-45 145
Msa0506530 MtrunA17_Chr8g0362321 45.205 146 75 1 2 142 5 150 4.64e-45 144
Msa0506530 MtrunA17_Chr3g0131871 48.611 144 72 2 5 146 6 149 3.81e-42 138
Msa0506530 MtrunA17_Chr3g0139901 40.136 147 84 3 5 147 7 153 3.41e-34 117
Msa0506530 MtrunA17_Chr4g0038891 44.355 124 69 0 4 127 35 158 6.03e-34 117
Msa0506530 MtrunA17_Chr1g0199951 45.669 127 68 1 3 128 43 169 5.00e-33 115
Msa0506530 MtrunA17_Chr5g0396451 41.406 128 75 0 4 131 35 162 3.75e-32 112
Msa0506530 MtrunA17_Chr4g0053191 36.842 152 89 2 1 145 4 155 1.66e-30 107
Msa0506530 MtrunA17_Chr4g0031181 35.821 134 72 2 15 134 18 151 2.23e-27 100
Msa0506530 MtrunA17_Chr5g0401831 33.553 152 94 2 1 145 4 155 3.44e-27 99.4
Msa0506530 MtrunA17_Chr8g0348701 37.398 123 75 2 26 146 25 147 7.68e-27 99.4
Msa0506530 MtrunA17_Chr5g0403591 36.765 136 83 2 5 139 33 166 2.15e-26 98.2
Msa0506530 MtrunA17_Chr7g0254701 34.965 143 93 0 4 146 13 155 2.55e-25 97.4
Msa0506530 MtrunA17_Chr8g0387131 35.036 137 87 2 12 146 11 147 4.13e-25 94.7
Msa0506530 MtrunA17_Chr2g0328001 34.028 144 81 2 15 144 18 161 6.95e-25 93.6
Msa0506530 MtrunA17_Chr4g0059291 30.894 123 83 2 26 146 25 147 2.41e-22 87.8
Msa0506530 MtrunA17_Chr8g0372391 38.835 103 61 2 1 102 15 116 3.67e-19 79.0
Msa0506530 MtrunA17_Chr4g0068301 34.454 119 68 4 2 114 11 125 2.13e-18 79.3
Msa0506530 MtrunA17_Chr8g0376111 31.373 153 87 5 4 144 43 189 3.56e-18 79.3
Msa0506530 MtrunA17_Chr5g0404931 36.893 103 63 2 1 102 15 116 5.90e-18 75.5
Msa0506530 MtrunA17_Chr7g0233631 36.885 122 67 3 4 115 38 159 3.02e-17 76.6
Msa0506530 MtrunA17_Chr7g0233611 36.885 122 67 3 4 115 38 159 6.76e-17 75.5
Msa0506530 MtrunA17_Chr8g0345331 32.479 117 70 3 4 115 8 120 5.17e-16 72.0
Msa0506530 MtrunA17_Chr7g0222941 30.894 123 75 3 3 115 90 212 7.54e-15 68.9
Msa0506530 MtrunA17_Chr4g0047301 30.667 150 91 4 4 143 672 818 1.20e-14 70.1
Msa0506530 MtrunA17_Chr4g0045101 32.787 122 72 3 4 115 37 158 3.41e-14 68.2
Msa0506530 MtrunA17_Chr2g0282211 28.289 152 96 4 3 144 681 829 1.05e-13 67.4
Msa0506530 MtrunA17_Chr1g0186291 29.293 99 56 2 43 140 30 115 8.78e-13 61.6
Msa0506530 MtrunA17_Chr2g0317631 30.081 123 76 3 3 115 306 428 1.24e-12 64.3
Msa0506530 MtrunA17_Chr8g0376201 31.707 123 74 3 3 115 192 314 2.39e-12 63.2
Msa0506530 MtrunA17_Chr1g0198021 28.455 123 78 3 3 115 137 259 3.16e-12 62.8
Msa0506530 MtrunA17_Chr1g0211131 29.508 122 76 3 4 115 823 944 5.52e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0506530 AT5G41700.5 96.622 148 5 0 1 148 1 148 2.45e-107 302
Msa0506530 AT5G41700.2 96.622 148 5 0 1 148 1 148 2.45e-107 302
Msa0506530 AT5G41700.1 96.622 148 5 0 1 148 1 148 2.45e-107 302
Msa0506530 AT1G64230.2 95.946 148 6 0 1 148 1 148 2.77e-107 302
Msa0506530 AT1G64230.1 95.946 148 6 0 1 148 1 148 2.77e-107 302
Msa0506530 AT5G53300.2 95.946 148 6 0 1 148 1 148 8.38e-107 301
Msa0506530 AT5G53300.4 95.946 148 6 0 1 148 1 148 8.38e-107 301
Msa0506530 AT5G53300.1 95.946 148 6 0 1 148 1 148 8.38e-107 301
Msa0506530 AT4G27960.1 95.270 148 7 0 1 148 1 148 1.73e-106 300
Msa0506530 AT4G27960.2 95.270 148 7 0 1 148 31 178 5.81e-106 300
Msa0506530 AT3G08690.2 93.919 148 9 0 1 148 1 148 6.12e-105 296
Msa0506530 AT3G08690.1 93.919 148 9 0 1 148 1 148 6.12e-105 296
Msa0506530 AT5G41700.4 95.302 149 6 1 1 148 1 149 1.58e-104 295
Msa0506530 AT1G64230.3 89.809 157 7 1 1 148 1 157 1.05e-102 291
Msa0506530 AT5G56150.1 89.189 148 16 0 1 148 1 148 1.26e-100 285
Msa0506530 AT2G16740.1 87.755 147 18 0 1 147 1 147 7.55e-99 281
Msa0506530 AT1G64230.5 74.211 190 7 1 1 148 1 190 2.92e-98 281
Msa0506530 AT1G64230.4 74.211 190 7 1 1 148 1 190 2.92e-98 281
Msa0506530 AT3G08700.2 79.195 149 30 1 1 148 1 149 3.19e-87 251
Msa0506530 AT3G08700.1 79.195 149 30 1 1 148 1 149 3.19e-87 251
Msa0506530 AT5G41700.3 96.154 104 4 0 1 104 1 104 1.37e-72 214
Msa0506530 AT5G53300.3 93.578 109 5 1 1 107 1 109 9.94e-72 211
Msa0506530 AT5G56150.2 87.379 103 13 0 1 103 1 103 3.66e-66 197
Msa0506530 AT3G13550.1 48.276 145 75 0 2 146 37 181 2.36e-53 167
Msa0506530 AT1G16890.2 49.655 145 73 0 4 148 8 152 3.27e-48 153
Msa0506530 AT1G78870.2 48.966 145 74 0 4 148 8 152 2.97e-47 150
Msa0506530 AT5G62540.1 49.254 134 68 0 2 135 5 138 7.75e-46 147
Msa0506530 AT1G16890.3 47.403 154 72 1 4 148 8 161 1.50e-45 146
Msa0506530 AT1G14400.1 46.575 146 73 1 2 142 5 150 1.66e-45 146
Msa0506530 AT1G14400.2 46.575 146 73 1 2 142 5 150 1.66e-45 146
Msa0506530 AT2G02760.1 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0506530 AT2G02760.2 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0506530 AT2G02760.4 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0506530 AT2G02760.3 45.890 146 74 1 2 142 5 150 3.63e-45 145
Msa0506530 AT1G36340.2 48.966 145 73 1 2 146 9 152 3.82e-45 145
Msa0506530 AT1G36340.1 48.966 145 73 1 2 146 9 152 3.82e-45 145
Msa0506530 AT3G13550.2 50.000 114 57 0 2 115 37 150 2.99e-42 138
Msa0506530 AT1G16890.1 52.101 119 57 0 30 148 1 119 4.40e-41 134
Msa0506530 AT1G78870.4 52.101 119 57 0 30 148 1 119 7.69e-41 133
Msa0506530 AT5G50870.1 45.833 144 76 2 5 146 6 149 1.88e-39 132
Msa0506530 AT1G78870.1 51.667 120 57 1 30 148 1 120 4.60e-39 128
Msa0506530 AT2G32790.1 49.342 152 68 4 2 148 30 177 2.34e-36 123
Msa0506530 AT5G50870.2 42.581 155 76 3 5 146 6 160 4.83e-36 123
Msa0506530 AT3G24515.1 44.586 157 74 3 3 146 8 164 2.26e-35 126
Msa0506530 AT3G24515.3 46.721 122 61 1 29 146 68 189 1.01e-32 120
Msa0506530 AT5G25760.1 38.776 147 86 3 5 147 7 153 1.11e-32 113
Msa0506530 AT5G25760.2 38.776 147 86 3 5 147 7 153 1.11e-32 113
Msa0506530 AT1G78870.3 48.571 105 54 0 4 108 8 112 2.15e-32 111
Msa0506530 AT3G20060.1 42.742 124 71 0 4 127 39 162 7.40e-31 109
Msa0506530 AT1G50490.1 43.548 124 70 0 4 127 38 161 2.22e-30 108
Msa0506530 AT3G20060.2 43.636 110 62 0 22 131 12 121 2.80e-29 104
Msa0506530 AT2G46030.5 36.486 148 91 3 1 146 1 147 2.64e-28 103
Msa0506530 AT2G46030.1 36.486 148 91 3 1 146 1 147 2.64e-28 103
Msa0506530 AT3G57870.1 34.868 152 92 2 1 145 5 156 4.56e-28 102
Msa0506530 AT2G46030.3 36.486 148 91 3 1 146 73 219 6.57e-28 103
Msa0506530 AT5G05080.2 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0506530 AT5G05080.3 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0506530 AT5G05080.1 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0506530 AT5G05080.4 37.063 143 90 0 4 146 13 155 8.50e-28 103
Msa0506530 AT2G46030.4 39.024 123 73 2 26 146 28 150 9.73e-28 102
Msa0506530 AT2G46030.2 39.024 123 73 2 26 146 28 150 9.73e-28 102
Msa0506530 AT3G55380.1 36.567 134 71 2 15 134 19 152 8.26e-27 99.0
Msa0506530 AT5G41340.1 38.211 123 74 2 26 146 25 147 1.03e-26 99.4
Msa0506530 AT1G63800.2 38.211 123 74 2 26 146 10 132 1.22e-26 98.6
Msa0506530 AT1G63800.1 38.211 123 74 2 26 146 25 147 1.33e-26 99.0
Msa0506530 AT5G59300.1 34.722 144 80 2 15 144 18 161 2.36e-25 95.1
Msa0506530 AT4G36800.2 35.036 137 84 3 5 139 34 167 4.15e-25 95.1
Msa0506530 AT4G36800.1 35.036 137 84 3 5 139 34 167 4.15e-25 95.1
Msa0506530 AT3G46460.1 34.028 144 81 2 15 144 18 161 4.23e-25 94.7
Msa0506530 AT5G41340.2 40.385 104 60 2 45 146 9 112 1.42e-23 90.1
Msa0506530 AT2G18600.1 35.036 137 84 3 5 139 35 168 2.65e-23 90.5
Msa0506530 AT2G18600.2 35.036 137 84 3 5 139 35 168 2.65e-23 90.5
Msa0506530 AT3G17000.1 36.975 119 65 4 2 114 12 126 2.08e-20 85.5
Msa0506530 AT3G55380.2 28.994 169 72 3 15 135 19 187 1.33e-19 81.3
Msa0506530 AT1G75440.1 38.889 108 60 3 1 107 15 117 8.37e-19 78.2
Msa0506530 AT1G45050.1 37.864 103 62 2 1 102 15 116 2.80e-18 77.0
Msa0506530 AT5G42990.1 38.835 103 61 2 1 102 15 116 4.39e-18 76.3
Msa0506530 AT1G17280.9 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT1G17280.7 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT1G17280.6 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT1G17280.2 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT1G17280.5 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT1G17280.3 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT1G17280.1 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT1G17280.8 34.188 117 68 3 4 115 8 120 3.63e-17 75.5
Msa0506530 AT4G36410.1 35.185 108 64 3 1 107 15 117 3.70e-17 73.9
Msa0506530 AT5G50430.1 34.188 117 68 3 4 115 8 120 4.45e-17 75.5
Msa0506530 AT5G50430.2 34.188 117 68 3 4 115 8 120 4.45e-17 75.5
Msa0506530 AT3G46460.3 30.631 111 64 1 47 144 9 119 1.19e-16 71.6
Msa0506530 AT3G46460.2 30.631 111 64 1 47 144 9 119 1.19e-16 71.6
Msa0506530 AT1G17280.4 31.707 123 69 3 4 115 8 126 8.56e-16 72.0
Msa0506530 AT3G15355.1 28.289 152 96 4 3 144 334 482 1.17e-11 61.6
Msa0506530 AT1G45050.2 34.831 89 56 2 1 88 15 102 1.27e-11 58.5
Msa0506530 AT1G53025.2 30.328 122 75 3 4 115 274 395 2.75e-11 60.5
Msa0506530 AT1G53025.1 30.328 122 75 3 4 115 274 395 3.07e-11 60.5
Msa0506530 AT1G53023.1 31.148 122 74 3 4 115 60 181 6.51e-11 59.3

Find 92 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAGAGAATGAGAGAAAA+AGG 0.204795 3_4:+52962964 None:intergenic
CAATACTGCAGGTTGCATTT+AGG 0.246319 3_4:-52961030 Msa0506530:intron
CTTCCATTGCTGTTGATGTT+TGG 0.284673 3_4:+52960992 None:intergenic
AATCGAAAGAAGGGATAATA+AGG 0.289702 3_4:+52962943 None:intergenic
TTGTACATATGTGCAATTTC+AGG 0.297625 3_4:+52960232 None:intergenic
TTCCATTGCTGTTGATGTTT+GGG 0.299216 3_4:+52960993 None:intergenic
ACACGGTCCATGCATTAAAA+TGG 0.325776 3_4:+52959645 None:intergenic
AATACCTTTGGTGGCTTGAA+TGG 0.326318 3_4:+52962278 None:intergenic
GTGAAAATTCTTAAGACATC+TGG 0.326730 3_4:-52960010 Msa0506530:three_prime_UTR
ATTTGTATACCAACATTATA+AGG 0.326922 3_4:-52959670 Msa0506530:three_prime_UTR
TTAATGCATGGACCGTGTTT+TGG 0.328281 3_4:-52959640 None:intergenic
CACAAGCGGGTCATCGGGAT+TGG 0.353907 3_4:+52960255 None:intergenic
ATCTTCTCTGTTTGTCGTAA+TGG 0.356690 3_4:+52963021 None:intergenic
TATTTGTCTCGACATTCTTA+AGG 0.371764 3_4:-52960969 Msa0506530:CDS
GTCAATCTGCTCATTGTTGA+CGG 0.386180 3_4:-52960281 Msa0506530:CDS
TTTAACAGCGAAGGAGGAAT+TGG 0.399882 3_4:+52960145 None:intergenic
GCGTGCGGTGGCTTCATACT+TGG 0.404055 3_4:+52960198 None:intergenic
GGTGGCTTGAATGGATAATC+AGG 0.410769 3_4:+52962287 None:intergenic
ATTAGGGTTGAAATTGAAAA+GGG 0.420801 3_4:+52962895 None:intergenic
TTCATTTATCATCGCTGCTT+GGG 0.423487 3_4:+52959956 None:intergenic
TGTCGTAATGGAATGTGTTT+GGG 0.427923 3_4:+52963033 None:intergenic
TTGTCGTAATGGAATGTGTT+TGG 0.428517 3_4:+52963032 None:intergenic
ACAAGCGGGTCATCGGGATT+GGG 0.433760 3_4:+52960256 None:intergenic
ACTAAATCAAACCTTGGAAA+TGG 0.438561 3_4:+52960925 None:intergenic
TCAGGCACAAGCGGGTCATC+GGG 0.450503 3_4:+52960250 None:intergenic
AGAAGAAGAAAATCGAAAGA+AGG 0.453266 3_4:+52962933 None:intergenic
GAAGAAGAAAATCGAAAGAA+GGG 0.456140 3_4:+52962934 None:intergenic
ATGAAGCCACCGCACGCAGC+TGG 0.456609 3_4:-52960192 Msa0506530:CDS
TAGGGTTTCAATACCTTTGG+TGG 0.458014 3_4:+52962269 None:intergenic
GATTAGGGTTGAAATTGAAA+AGG 0.459907 3_4:+52962894 None:intergenic
TTCAGGCACAAGCGGGTCAT+CGG 0.461878 3_4:+52960249 None:intergenic
TTTGTTTATATGTTATCTGC+TGG 0.463714 3_4:-52959867 Msa0506530:three_prime_UTR
TTTCATTTATCATCGCTGCT+TGG 0.470863 3_4:+52959955 None:intergenic
AACAAACATAATTCAATCTC+AGG 0.474332 3_4:+52959884 None:intergenic
TGGAACTCAACCATGGCAAT+GGG 0.477736 3_4:-52960049 Msa0506530:three_prime_UTR
GCAGGTTGCATTTAGGACTA+AGG 0.478226 3_4:-52961023 Msa0506530:intron
ACCTTGGAAATGGTCAGTGC+AGG 0.479512 3_4:+52960935 None:intergenic
AAAATGGCTCCTTATAATGT+TGG 0.479743 3_4:+52959661 None:intergenic
TCATTGGCAAGCAACAATCA+TGG 0.479981 3_4:-52962366 Msa0506530:CDS
GTGACCTTACCTTACGCAAA+AGG 0.491996 3_4:-52963142 None:intergenic
TCTCCCCTTTGATTTGAGCA+TGG 0.493500 3_4:-52962847 Msa0506530:exon
TGGCTGAGGACATGTTTCAT+TGG 0.495052 3_4:-52962382 Msa0506530:CDS
CGATGCCATGCTCAAATCAA+AGG 0.498135 3_4:+52962842 None:intergenic
TGACCGGTACCAGCTTCAAC+TGG 0.505717 3_4:-52959764 Msa0506530:three_prime_UTR
CATTGCTGTTGATGTTTGGG+TGG 0.507557 3_4:+52960996 None:intergenic
CATTGGCAAGCAACAATCAT+GGG 0.510542 3_4:-52962365 Msa0506530:CDS
CCCTGCACTGACCATTTCCA+AGG 0.514922 3_4:-52960936 Msa0506530:intron
CCACCAACATCATGCAGTGC+AGG 0.515198 3_4:-52962780 Msa0506530:intron
ATGGAACTCAACCATGGCAA+TGG 0.520736 3_4:-52960050 Msa0506530:three_prime_UTR
CCTGCACTGCATGATGTTGG+TGG 0.521666 3_4:+52962780 None:intergenic
AAAACACACCACCAGCATAA+GGG 0.532990 3_4:+52962330 None:intergenic
ATTTGAGCATGGCATCGAAA+CGG 0.533369 3_4:-52962836 Msa0506530:exon
CTGTGTCCAGCTGCGTGCGG+TGG 0.538680 3_4:+52960186 None:intergenic
TCACCTGCACTGCATGATGT+TGG 0.540978 3_4:+52962777 None:intergenic
CACATATGTACAAGACTGAC+AGG 0.541007 3_4:-52960222 Msa0506530:CDS
GATGCCATGCTCAAATCAAA+GGG 0.547382 3_4:+52962843 None:intergenic
AGCTGGTACCGGTCAACAGA+TGG 0.565098 3_4:+52959772 None:intergenic
CCTGCTGATAGCCCTTATGC+TGG 0.566650 3_4:-52962341 Msa0506530:CDS
CCAGCATAAGGGCTATCAGC+AGG 0.567868 3_4:+52962341 None:intergenic
TTACATAAAACATAAATGCA+CGG 0.568066 3_4:+52960117 None:intergenic
TAGTTGGTCCATCTGTTGAC+CGG 0.570984 3_4:-52959780 Msa0506530:three_prime_UTR
CACCCAAACATCAACAGCAA+TGG 0.573454 3_4:-52960995 Msa0506530:CDS
TGAAACATGTCCTCAGCCAC+AGG 0.579671 3_4:+52962386 None:intergenic
TAAGCTATAGACATATGAGT+GGG 0.581170 3_4:+52959724 None:intergenic
GTACCAGTTGAAGCTGGTAC+CGG 0.581776 3_4:+52959761 None:intergenic
GTGCAATTTCAGGCACAAGC+GGG 0.582074 3_4:+52960242 None:intergenic
CCTTGGAAATGGTCAGTGCA+GGG 0.590131 3_4:+52960936 None:intergenic
ATAATCAATTCCCATTGCCA+TGG 0.591438 3_4:+52960039 None:intergenic
CTGGACACAGAAGTATGCCA+TGG 0.597546 3_4:-52960173 Msa0506530:CDS
GCAAATCCTTGAGCTCCTTG+AGG 0.601494 3_4:+52962811 None:intergenic
ACGGTTGCATTTAACAGCGA+AGG 0.603135 3_4:+52960136 None:intergenic
GTTGCATTTAACAGCGAAGG+AGG 0.604567 3_4:+52960139 None:intergenic
ATGACTGTACCAGTTGAAGC+TGG 0.606662 3_4:+52959755 None:intergenic
ACGGATCCTCAAGGAGCTCA+AGG 0.606874 3_4:-52962817 Msa0506530:CDS
AGGAGGAATTGGTCATCCCA+TGG 0.622867 3_4:+52960156 None:intergenic
TCGACATTCTTAAGGAACAA+TGG 0.626316 3_4:-52960961 Msa0506530:CDS
GGAGCTCAAGGATTTGCAGA+AGG 0.628349 3_4:-52962805 Msa0506530:CDS
GCTGATAGCCCTTATGCTGG+TGG 0.629848 3_4:-52962338 Msa0506530:CDS
TTTGTTATTTATTGTCAACA+TGG 0.637302 3_4:-52960069 Msa0506530:three_prime_UTR
GAAAATTGTAGTTACGAACA+TGG 0.643887 3_4:-52959936 Msa0506530:three_prime_UTR
GGCATCGAAACGGATCCTCA+AGG 0.650007 3_4:-52962826 Msa0506530:CDS
TGGACACAGAAGTATGCCAT+GGG 0.652544 3_4:-52960172 Msa0506530:CDS
TTAAGCTATAGACATATGAG+TGG 0.658008 3_4:+52959723 None:intergenic
AGGAACAACGAAACATCCAC+AGG 0.659714 3_4:+52959904 None:intergenic
TGTGCAATTTCAGGCACAAG+CGG 0.661456 3_4:+52960241 None:intergenic
GGCTTGAATGGATAATCAGG+AGG 0.662651 3_4:+52962290 None:intergenic
AACATGGTGATTATTGCCTG+TGG 0.665031 3_4:-52959920 Msa0506530:three_prime_UTR
TTATCCATTCAAGCCACCAA+AGG 0.668003 3_4:-52962282 Msa0506530:intron
GTCAACATGGAACTCAACCA+TGG 0.686063 3_4:-52960056 Msa0506530:three_prime_UTR
CTTCTGTGTCCAGCTGCGTG+CGG 0.688818 3_4:+52960183 None:intergenic
AAGCTATAGACATATGAGTG+GGG 0.711204 3_4:+52959725 None:intergenic
ATGCCATGCTCAAATCAAAG+GGG 0.742920 3_4:+52962844 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATGATAAAAGAAAAAAA+GGG + chr3_4:52960045-52960064 None:intergenic 10.0%
!! TAAATGCTAATTATTATATT+TGG - chr3_4:52960914-52960933 Msa0506530:intron 10.0%
!! GATAATTAATGATTTATATA+AGG - chr3_4:52961878-52961897 Msa0506530:intron 10.0%
!! CTATATGATAAAAGAAAAAA+AGG + chr3_4:52960046-52960065 None:intergenic 15.0%
!! AAAACAATAAAACAACAAAA+GGG + chr3_4:52960162-52960181 None:intergenic 15.0%
!! AAAAACAATAAAACAACAAA+AGG + chr3_4:52960163-52960182 None:intergenic 15.0%
!!! TTTTTATCAATGATTTGTTA+TGG - chr3_4:52960178-52960197 Msa0506530:CDS 15.0%
!! AACATATCTAACAAATAATT+TGG + chr3_4:52960270-52960289 None:intergenic 15.0%
!!! ATTATTTGTTAGATATGTTA+TGG - chr3_4:52960271-52960290 Msa0506530:CDS 15.0%
!!! TTTGAAAGTTTTATTCTTTT+TGG - chr3_4:52960613-52960632 Msa0506530:intron 15.0%
!! GAAAACAGAAAAAAATAATA+TGG + chr3_4:52960781-52960800 None:intergenic 15.0%
!!! ATTTTTAAAAAATCACTATG+AGG - chr3_4:52960813-52960832 Msa0506530:intron 15.0%
!! TAGTATAAATAAGAAATTGT+AGG + chr3_4:52961099-52961118 None:intergenic 15.0%
!!! TATTTTTTTCAATTAACACA+GGG + chr3_4:52961300-52961319 None:intergenic 15.0%
!!! TTATTTTTTTCAATTAACAC+AGG + chr3_4:52961301-52961320 None:intergenic 15.0%
!! TTGAAAAAAATAATGATCAT+TGG - chr3_4:52961308-52961327 Msa0506530:intron 15.0%
!!! ATAATTTGTTGAGATTTGTT+TGG - chr3_4:52960554-52960573 Msa0506530:intron 20.0%
!! AAAAAATAATGATCATTGGT+TGG - chr3_4:52961312-52961331 Msa0506530:intron 20.0%
!! TTTGTTAATTGTTTGGAAAA+TGG - chr3_4:52961917-52961936 Msa0506530:intron 20.0%
!!! AAATCATGAATCATTTTCTT+GGG + chr3_4:52962139-52962158 None:intergenic 20.0%
!! TTAAATTCCTATCAATCATA+TGG + chr3_4:52962334-52962353 None:intergenic 20.0%
!! TTACATAAAACATAAATGCA+CGG + chr3_4:52962655-52962674 None:intergenic 20.0%
!!! TTTGTTATTTATTGTCAACA+TGG - chr3_4:52962700-52962719 Msa0506530:intron 20.0%
!! ATTTGTATACCAACATTATA+AGG - chr3_4:52963099-52963118 Msa0506530:five_prime_UTR 20.0%
! ATTAGGGTTGAAATTGAAAA+GGG + chr3_4:52959877-52959896 None:intergenic 25.0%
!! TTATGGATCTGTTATTTGAA+GGG - chr3_4:52960195-52960214 Msa0506530:CDS 25.0%
!!! ATGATGATGTTTTGTGTTTT+AGG - chr3_4:52960359-52960378 Msa0506530:intron 25.0%
! TTTAAAAAATCACTATGAGG+AGG - chr3_4:52960816-52960835 Msa0506530:intron 25.0%
! TTGAGATAATTAAAGAGCAA+GGG + chr3_4:52961136-52961155 None:intergenic 25.0%
! TACACTTTAATCAATGAATC+AGG - chr3_4:52961944-52961963 Msa0506530:intron 25.0%
!! CAAATCATGAATCATTTTCT+TGG + chr3_4:52962140-52962159 None:intergenic 25.0%
!!! AGAAGAACTGTGATTTTTTT+TGG - chr3_4:52962185-52962204 Msa0506530:intron 25.0%
!! ATCCTAACATTCACTATTTT+AGG - chr3_4:52962458-52962477 Msa0506530:intron 25.0%
! AACAAACATAATTCAATCTC+AGG + chr3_4:52962888-52962907 None:intergenic 25.0%
! TTTGTTTATATGTTATCTGC+TGG - chr3_4:52962902-52962921 Msa0506530:five_prime_UTR 25.0%
!! AGTTGTTGAGATAGATTTTT+AGG + chr3_4:52962963-52962982 None:intergenic 25.0%
!!! AGGTATTTTTAAAGCGTGTT+TGG - chr3_4:52959647-52959666 Msa0506530:three_prime_UTR 30.0%
! CTAAATTACGCGTTTTTGAA+TGG + chr3_4:52959700-52959719 None:intergenic 30.0%
AATCGAAAGAAGGGATAATA+AGG + chr3_4:52959829-52959848 None:intergenic 30.0%
GAAGAAGAAAATCGAAAGAA+GGG + chr3_4:52959838-52959857 None:intergenic 30.0%
AGAAGAAGAAAATCGAAAGA+AGG + chr3_4:52959839-52959858 None:intergenic 30.0%
GATTAGGGTTGAAATTGAAA+AGG + chr3_4:52959878-52959897 None:intergenic 30.0%
! GTTATGGATCTGTTATTTGA+AGG - chr3_4:52960194-52960213 Msa0506530:CDS 30.0%
!! TGTTATTTGAAGGGTTTTTG+AGG - chr3_4:52960204-52960223 Msa0506530:CDS 30.0%
!!! TTTTGAGGTGATTTGGATTT+AGG - chr3_4:52960219-52960238 Msa0506530:CDS 30.0%
! TTTGAGGTGATTTGGATTTA+GGG - chr3_4:52960220-52960239 Msa0506530:CDS 30.0%
GGTTGTTTACATGAAACTTT+AGG - chr3_4:52960241-52960260 Msa0506530:CDS 30.0%
ATGTTATGGTTTATGAAGCT+AGG - chr3_4:52960285-52960304 Msa0506530:CDS 30.0%
AAAAATAATATGGTCCCTGT+CGG + chr3_4:52960771-52960790 None:intergenic 30.0%
! AGCTTGATCGATAATTTTGA+CGG + chr3_4:52960942-52960961 None:intergenic 30.0%
ACTATTATCAAGTCCTTACT+TGG + chr3_4:52961063-52961082 None:intergenic 30.0%
ATAATTAAAGAGCAAGGGAA+TGG + chr3_4:52961131-52961150 None:intergenic 30.0%
CTTGAGATAATTAAAGAGCA+AGG + chr3_4:52961137-52961156 None:intergenic 30.0%
AGATCAGTGGAAAACAAAAA+AGG + chr3_4:52961361-52961380 None:intergenic 30.0%
GTCAATATTAACAACATCCA+CGG + chr3_4:52961542-52961561 None:intergenic 30.0%
AACATAAAACTATGTTCAGG+TGG + chr3_4:52961571-52961590 None:intergenic 30.0%
CCTAACATAAAACTATGTTC+AGG + chr3_4:52961574-52961593 None:intergenic 30.0%
!!! CCTGAACATAGTTTTATGTT+AGG - chr3_4:52961571-52961590 Msa0506530:intron 30.0%
! TTTTCTTTTTCTTGCTGATG+AGG - chr3_4:52961598-52961617 Msa0506530:intron 30.0%
GTTTGCAAACTCAATTAAAC+CGG + chr3_4:52961664-52961683 None:intergenic 30.0%
TTTGCAAACATCATGTAGTT+TGG - chr3_4:52961675-52961694 Msa0506530:intron 30.0%
TATTTGTCTCGACATTCTTA+AGG - chr3_4:52961800-52961819 Msa0506530:intron 30.0%
ACTAAATCAAACCTTGGAAA+TGG + chr3_4:52961847-52961866 None:intergenic 30.0%
ACAACAACTAAATCAAACCT+TGG + chr3_4:52961853-52961872 None:intergenic 30.0%
!! GACAAGCTTTGTTAATTGTT+TGG - chr3_4:52961910-52961929 Msa0506530:intron 30.0%
! TTTTGTTGATGTCATTCTTG+TGG - chr3_4:52962077-52962096 Msa0506530:intron 30.0%
TTTGTTGATGTCATTCTTGT+GGG - chr3_4:52962078-52962097 Msa0506530:intron 30.0%
AAGTTGACCATATGATTGAT+AGG - chr3_4:52962324-52962343 Msa0506530:CDS 30.0%
CACCTAAAATAGTGAATGTT+AGG + chr3_4:52962463-52962482 None:intergenic 30.0%
TTGTACATATGTGCAATTTC+AGG + chr3_4:52962540-52962559 None:intergenic 30.0%
GTGAAAATTCTTAAGACATC+TGG - chr3_4:52962759-52962778 Msa0506530:intron 30.0%
GAAAATTGTAGTTACGAACA+TGG - chr3_4:52962833-52962852 Msa0506530:exon 30.0%
!!! GTTGAGATAGATTTTTAGGT+TGG + chr3_4:52962959-52962978 None:intergenic 30.0%
TAAGCTATAGACATATGAGT+GGG + chr3_4:52963048-52963067 None:intergenic 30.0%
TTAAGCTATAGACATATGAG+TGG + chr3_4:52963049-52963068 None:intergenic 30.0%
AATCAACCAAAAACAAGACT+AGG + chr3_4:52963082-52963101 None:intergenic 30.0%
AAAATGGCTCCTTATAATGT+TGG + chr3_4:52963111-52963130 None:intergenic 30.0%
! TGTCGTAATGGAATGTGTTT+GGG + chr3_4:52959739-52959758 None:intergenic 35.0%
! TTGTCGTAATGGAATGTGTT+TGG + chr3_4:52959740-52959759 None:intergenic 35.0%
ATCTTCTCTGTTTGTCGTAA+TGG + chr3_4:52959751-52959770 None:intergenic 35.0%
GAGAGAGAATGAGAGAAAAA+GGG + chr3_4:52959807-52959826 None:intergenic 35.0%
CAGATCATACATACAAGCTA+GGG + chr3_4:52960521-52960540 None:intergenic 35.0%
ACAGATCATACATACAAGCT+AGG + chr3_4:52960522-52960541 None:intergenic 35.0%
AAAATAATATGGTCCCTGTC+GGG + chr3_4:52960770-52960789 None:intergenic 35.0%
!! GCTTGATCGATAATTTTGAC+GGG + chr3_4:52960941-52960960 None:intergenic 35.0%
!! AAGCAAACTTTTTCTGAAGG+CGG + chr3_4:52960965-52960984 None:intergenic 35.0%
!! GAGAAGCAAACTTTTTCTGA+AGG + chr3_4:52960968-52960987 None:intergenic 35.0%
TATTTGTGTTGCAACTTGCA+AGG - chr3_4:52961210-52961229 Msa0506530:intron 35.0%
TTATTGTCTTGCAATACTGC+AGG - chr3_4:52961728-52961747 Msa0506530:intron 35.0%
! TTCCATTGCTGTTGATGTTT+GGG + chr3_4:52961779-52961798 None:intergenic 35.0%
TCGACATTCTTAAGGAACAA+TGG - chr3_4:52961808-52961827 Msa0506530:intron 35.0%
AACAACAACATTCCAAATGC+AGG + chr3_4:52962398-52962417 None:intergenic 35.0%
! GTTGTTGTTCTTATGTGTCA+TGG - chr3_4:52962409-52962428 Msa0506530:intron 35.0%
ATAATCAATTCCCATTGCCA+TGG + chr3_4:52962733-52962752 None:intergenic 35.0%
TTCATTTATCATCGCTGCTT+GGG + chr3_4:52962816-52962835 None:intergenic 35.0%
TTTCATTTATCATCGCTGCT+TGG + chr3_4:52962817-52962836 None:intergenic 35.0%
! CTCAACAACTTTTCTGTAGT+TGG - chr3_4:52962973-52962992 Msa0506530:five_prime_UTR 35.0%
AAGCTATAGACATATGAGTG+GGG + chr3_4:52963047-52963066 None:intergenic 35.0%
!! TAAGGAGCCATTTTAATGCA+TGG - chr3_4:52963117-52963136 Msa0506530:five_prime_UTR 35.0%
AGAATGAGAGAAAAAGGGAC+AGG + chr3_4:52959802-52959821 None:intergenic 40.0%
GGAGAGAGAATGAGAGAAAA+AGG + chr3_4:52959808-52959827 None:intergenic 40.0%
AGAGAGAGAGAGAGAGATTA+GGG + chr3_4:52959893-52959912 None:intergenic 40.0%
ATGCCATGCTCAAATCAAAG+GGG + chr3_4:52959928-52959947 None:intergenic 40.0%
GATGCCATGCTCAAATCAAA+GGG + chr3_4:52959929-52959948 None:intergenic 40.0%
! ATTTGAGCATGGCATCGAAA+CGG - chr3_4:52959933-52959952 Msa0506530:three_prime_UTR 40.0%
!!! GAAGGGTTTTTGAGGTGATT+TGG - chr3_4:52960212-52960231 Msa0506530:CDS 40.0%
!! GTTTTGTGTTTTAGGTCCTG+TGG - chr3_4:52960367-52960386 Msa0506530:intron 40.0%
TCATTGGCAAGCAACAATCA+TGG - chr3_4:52960403-52960422 Msa0506530:intron 40.0%
CATTGGCAAGCAACAATCAT+GGG - chr3_4:52960404-52960423 Msa0506530:intron 40.0%
AAAACACACCACCAGCATAA+GGG + chr3_4:52960442-52960461 None:intergenic 40.0%
AAAAACACACCACCAGCATA+AGG + chr3_4:52960443-52960462 None:intergenic 40.0%
!! TTATGCTGGTGGTGTGTTTT+TGG - chr3_4:52960442-52960461 Msa0506530:intron 40.0%
TTATCCATTCAAGCCACCAA+AGG - chr3_4:52960487-52960506 Msa0506530:intron 40.0%
! AATACCTTTGGTGGCTTGAA+TGG + chr3_4:52960494-52960513 None:intergenic 40.0%
TAGGGTTTCAATACCTTTGG+TGG + chr3_4:52960503-52960522 None:intergenic 40.0%
AGCTAGGGTTTCAATACCTT+TGG + chr3_4:52960506-52960525 None:intergenic 40.0%
ATCAAATCTATCTAGCCGTG+CGG - chr3_4:52960699-52960718 Msa0506530:intron 40.0%
!!! TGTTTTGAGGGGTGAATGTT+TGG - chr3_4:52960732-52960751 Msa0506530:intron 40.0%
GCAATGAAAGCTTTGCATCT+TGG + chr3_4:52960890-52960909 None:intergenic 40.0%
ATCTCAAGCAAACAAGCTAG+TGG - chr3_4:52961149-52961168 Msa0506530:intron 40.0%
TCTCAAGCAAACAAGCTAGT+GGG - chr3_4:52961150-52961169 Msa0506530:intron 40.0%
CAACAGTTTGCAATACACTC+AGG + chr3_4:52961269-52961288 None:intergenic 40.0%
AACTGGGACAAAAAGATCAG+TGG + chr3_4:52961374-52961393 None:intergenic 40.0%
TCTCAAACCAATGAAATCCG+TGG - chr3_4:52961522-52961541 Msa0506530:intron 40.0%
ACAACATCCACGGATTTCAT+TGG + chr3_4:52961532-52961551 None:intergenic 40.0%
CCAAGTACAGTATACTTGTC+CGG - chr3_4:52961642-52961661 Msa0506530:intron 40.0%
CAATACTGCAGGTTGCATTT+AGG - chr3_4:52961739-52961758 Msa0506530:intron 40.0%
! CTTCCATTGCTGTTGATGTT+TGG + chr3_4:52961780-52961799 None:intergenic 40.0%
TTGATGTCATTCTTGTGGGA+AGG - chr3_4:52962082-52962101 Msa0506530:intron 40.0%
ACACACGCAAACTATATCTC+AGG + chr3_4:52962295-52962314 None:intergenic 40.0%
CAAATGCAGGTCGTTAAACA+CGG + chr3_4:52962385-52962404 None:intergenic 40.0%
GTCAATCTGCTCATTGTTGA+CGG - chr3_4:52962488-52962507 Msa0506530:intron 40.0%
CACATATGTACAAGACTGAC+AGG - chr3_4:52962547-52962566 Msa0506530:intron 40.0%
TTTAACAGCGAAGGAGGAAT+TGG + chr3_4:52962627-52962646 None:intergenic 40.0%
! GACATCTGGTGCAAATTTTC+TGG - chr3_4:52962773-52962792 Msa0506530:intron 40.0%
AACATGGTGATTATTGCCTG+TGG - chr3_4:52962849-52962868 Msa0506530:exon 40.0%
!! AGGTTGGTAAGCAGCAAAAA+TGG + chr3_4:52962943-52962962 None:intergenic 40.0%
!!! CTCTGTCCTAGTCTTGTTTT+TGG - chr3_4:52963073-52963092 Msa0506530:five_prime_UTR 40.0%
ACACGGTCCATGCATTAAAA+TGG + chr3_4:52963127-52963146 None:intergenic 40.0%
GAGAGAGAGAGAGAGAGATT+AGG + chr3_4:52959894-52959913 None:intergenic 45.0%
!! TCTCCCCTTTGATTTGAGCA+TGG - chr3_4:52959922-52959941 Msa0506530:three_prime_UTR 45.0%
CGATGCCATGCTCAAATCAA+AGG + chr3_4:52959930-52959949 None:intergenic 45.0%
TGGCTGAGGACATGTTTCAT+TGG - chr3_4:52960387-52960406 Msa0506530:intron 45.0%
GGCTTGAATGGATAATCAGG+AGG + chr3_4:52960482-52960501 None:intergenic 45.0%
GGTGGCTTGAATGGATAATC+AGG + chr3_4:52960485-52960504 None:intergenic 45.0%
!! CTCGCAGAGGATCGATTTTA+TGG + chr3_4:52961034-52961053 None:intergenic 45.0%
CATAAAATCGATCCTCTGCG+AGG - chr3_4:52961032-52961051 Msa0506530:intron 45.0%
CTCAAGCAAACAAGCTAGTG+GGG - chr3_4:52961151-52961170 Msa0506530:intron 45.0%
TCTTGAGGCAGGTTTATGCT+AGG + chr3_4:52961180-52961199 None:intergenic 45.0%
AAATAGCCTGAGGCTTCTTG+AGG + chr3_4:52961195-52961214 None:intergenic 45.0%
GTTGCAACACAAATAGCCTG+AGG + chr3_4:52961205-52961224 None:intergenic 45.0%
TTGCAATACACTCAGGCTCT+CGG + chr3_4:52961262-52961281 None:intergenic 45.0%
ACATGCATGCAAGCGAAACT+GGG + chr3_4:52961390-52961409 None:intergenic 45.0%
AGACGACAATTGTGAACGTG+TGG + chr3_4:52961419-52961438 None:intergenic 45.0%
CCGGACAAGTATACTGTACT+TGG + chr3_4:52961645-52961664 None:intergenic 45.0%
! GCAGGTTGCATTTAGGACTA+AGG - chr3_4:52961746-52961765 Msa0506530:intron 45.0%
! CATTGCTGTTGATGTTTGGG+TGG + chr3_4:52961776-52961795 None:intergenic 45.0%
CACCCAAACATCAACAGCAA+TGG - chr3_4:52961774-52961793 Msa0506530:intron 45.0%
CGTGTTTAACGACCTGCATT+TGG - chr3_4:52962383-52962402 Msa0506530:CDS 45.0%
TGTGCAATTTCAGGCACAAG+CGG + chr3_4:52962531-52962550 None:intergenic 45.0%
! TGGACACAGAAGTATGCCAT+GGG - chr3_4:52962597-52962616 Msa0506530:intron 45.0%
GTTGCATTTAACAGCGAAGG+AGG + chr3_4:52962633-52962652 None:intergenic 45.0%
ACGGTTGCATTTAACAGCGA+AGG + chr3_4:52962636-52962655 None:intergenic 45.0%
GTCAACATGGAACTCAACCA+TGG - chr3_4:52962713-52962732 Msa0506530:intron 45.0%
ATGGAACTCAACCATGGCAA+TGG - chr3_4:52962719-52962738 Msa0506530:intron 45.0%
TGGAACTCAACCATGGCAAT+GGG - chr3_4:52962720-52962739 Msa0506530:intron 45.0%
AGGAACAACGAAACATCCAC+AGG + chr3_4:52962868-52962887 None:intergenic 45.0%
TAGTTGGTCCATCTGTTGAC+CGG - chr3_4:52962989-52963008 Msa0506530:five_prime_UTR 45.0%
ATGACTGTACCAGTTGAAGC+TGG + chr3_4:52963017-52963036 None:intergenic 45.0%
!!! ATGATTTATTTTTTAAATTA+TGG - chr3_4:52960133-52960152 Msa0506530:three_prime_UTR 5.0%
!!! TGATTTATTTTTTAAATTAT+GGG - chr3_4:52960134-52960153 Msa0506530:three_prime_UTR 5.0%
GAGAGAAAAAGGGACAGGCA+AGG + chr3_4:52959797-52959816 None:intergenic 50.0%
GCAAATCCTTGAGCTCCTTG+AGG + chr3_4:52959961-52959980 None:intergenic 50.0%
GGAGCTCAAGGATTTGCAGA+AGG - chr3_4:52959964-52959983 Msa0506530:three_prime_UTR 50.0%
TCACCTGCACTGCATGATGT+TGG + chr3_4:52959995-52960014 None:intergenic 50.0%
!! TGTTTTAGGTCCTGTGGCTG+AGG - chr3_4:52960373-52960392 Msa0506530:intron 50.0%
TGAAACATGTCCTCAGCCAC+AGG + chr3_4:52960386-52960405 None:intergenic 50.0%
AAATCTATCTAGCCGTGCGG+TGG - chr3_4:52960702-52960721 Msa0506530:intron 50.0%
TTGAGGGGTGAATGTTTGGC+AGG - chr3_4:52960736-52960755 Msa0506530:intron 50.0%
CTATGAGGAGGTTCGAACCA+TGG - chr3_4:52960828-52960847 Msa0506530:intron 50.0%
GTCGTGGTATTACAGCGCAT+CGG + chr3_4:52960866-52960885 None:intergenic 50.0%
GAGCAAGGGAATGGACGAAT+GGG + chr3_4:52961122-52961141 None:intergenic 50.0%
AGAGCAAGGGAATGGACGAA+TGG + chr3_4:52961123-52961142 None:intergenic 50.0%
ACCTTACCTCAATGCCGAAG+TGG - chr3_4:52961236-52961255 Msa0506530:intron 50.0%
CACATGCATGCAAGCGAAAC+TGG + chr3_4:52961391-52961410 None:intergenic 50.0%
CCTTGGAAATGGTCAGTGCA+GGG + chr3_4:52961836-52961855 None:intergenic 50.0%
ACCTTGGAAATGGTCAGTGC+AGG + chr3_4:52961837-52961856 None:intergenic 50.0%
GTGCAATTTCAGGCACAAGC+GGG + chr3_4:52962530-52962549 None:intergenic 50.0%
! CTGGACACAGAAGTATGCCA+TGG - chr3_4:52962596-52962615 Msa0506530:intron 50.0%
AGGAGGAATTGGTCATCCCA+TGG + chr3_4:52962616-52962635 None:intergenic 50.0%
GTACCAGTTGAAGCTGGTAC+CGG + chr3_4:52963011-52963030 None:intergenic 50.0%
! GGCATCGAAACGGATCCTCA+AGG - chr3_4:52959943-52959962 Msa0506530:three_prime_UTR 55.0%
ACGGATCCTCAAGGAGCTCA+AGG - chr3_4:52959952-52959971 Msa0506530:three_prime_UTR 55.0%
CCTGCACTGCATGATGTTGG+TGG + chr3_4:52959992-52960011 None:intergenic 55.0%
CCACCAACATCATGCAGTGC+AGG - chr3_4:52959989-52960008 Msa0506530:three_prime_UTR 55.0%
CCAGCATAAGGGCTATCAGC+AGG + chr3_4:52960431-52960450 None:intergenic 55.0%
CCTGCTGATAGCCCTTATGC+TGG - chr3_4:52960428-52960447 Msa0506530:intron 55.0%
GCTGATAGCCCTTATGCTGG+TGG - chr3_4:52960431-52960450 Msa0506530:intron 55.0%
! ATCGGTTTACCTCGCGTCCA+TGG + chr3_4:52960848-52960867 None:intergenic 55.0%
AGCTTTGCATCTTGGCGTCG+TGG + chr3_4:52960882-52960901 None:intergenic 55.0%
TACTTGGTACGACCTCGCAG+AGG + chr3_4:52961047-52961066 None:intergenic 55.0%
CTGCGAGGTCGTACCAAGTA+AGG - chr3_4:52961047-52961066 Msa0506530:intron 55.0%
AACCTGCCTCAAGAAGCCTC+AGG - chr3_4:52961186-52961205 Msa0506530:intron 55.0%
! GCCACTTCGGCATTGAGGTA+AGG + chr3_4:52961240-52961259 None:intergenic 55.0%
! CCCTGCACTGACCATTTCCA+AGG - chr3_4:52961833-52961852 Msa0506530:intron 55.0%
ACAAGCGGGTCATCGGGATT+GGG + chr3_4:52962516-52962535 None:intergenic 55.0%
TTCAGGCACAAGCGGGTCAT+CGG + chr3_4:52962523-52962542 None:intergenic 55.0%
!! AGCTGGTACCGGTCAACAGA+TGG + chr3_4:52963000-52963019 None:intergenic 55.0%
TGACCGGTACCAGCTTCAAC+TGG - chr3_4:52963005-52963024 Msa0506530:five_prime_UTR 55.0%
TCTATCTAGCCGTGCGGTGG+CGG - chr3_4:52960705-52960724 Msa0506530:intron 60.0%
!!! GGTGGCGGGGTCTGTTTTGA+GGG - chr3_4:52960720-52960739 Msa0506530:intron 60.0%
!! GTGGCGGGGTCTGTTTTGAG+GGG - chr3_4:52960721-52960740 Msa0506530:intron 60.0%
AGGTTCGAACCATGGACGCG+AGG - chr3_4:52960836-52960855 Msa0506530:intron 60.0%
AGCCTGAGGCTTCTTGAGGC+AGG + chr3_4:52961191-52961210 None:intergenic 60.0%
TCTCGGCCACTTCGGCATTG+AGG + chr3_4:52961245-52961264 None:intergenic 60.0%
ACTCAGGCTCTCGGCCACTT+CGG + chr3_4:52961253-52961272 None:intergenic 60.0%
CACAAGCGGGTCATCGGGAT+TGG + chr3_4:52962517-52962536 None:intergenic 60.0%
TCAGGCACAAGCGGGTCATC+GGG + chr3_4:52962522-52962541 None:intergenic 60.0%
GCGTGCGGTGGCTTCATACT+TGG + chr3_4:52962574-52962593 None:intergenic 60.0%
CTTCTGTGTCCAGCTGCGTG+CGG + chr3_4:52962589-52962608 None:intergenic 60.0%
CTATCTAGCCGTGCGGTGGC+GGG - chr3_4:52960706-52960725 Msa0506530:intron 65.0%
TATCTAGCCGTGCGGTGGCG+GGG - chr3_4:52960707-52960726 Msa0506530:intron 65.0%
AAACAGACCCCGCCACCGCA+CGG + chr3_4:52960717-52960736 None:intergenic 65.0%
!!! CGGTGGCGGGGTCTGTTTTG+AGG - chr3_4:52960719-52960738 Msa0506530:intron 65.0%
GCAGGCAGTCGATCCCGACA+GGG - chr3_4:52960754-52960773 Msa0506530:intron 65.0%
ATGAAGCCACCGCACGCAGC+TGG - chr3_4:52962577-52962596 Msa0506530:intron 65.0%
GGCAGGCAGTCGATCCCGAC+AGG - chr3_4:52960753-52960772 Msa0506530:intron 70.0%
CTGTGTCCAGCTGCGTGCGG+TGG + chr3_4:52962586-52962605 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 52959642 52963149 52959642 ID=Msa0506530;Name=Msa0506530
chr3_4 mRNA 52959642 52963149 52959642 ID=Msa0506530-mRNA-1;Parent=Msa0506530;Name=Msa0506530-mRNA-1;_AED=0.08;_eAED=0.08;_QI=299|1|1|1|1|1|4|526|148
chr3_4 exon 52959642 52960311 52959642 ID=Msa0506530-mRNA-1:exon:11663;Parent=Msa0506530-mRNA-1
chr3_4 exon 52960937 52961041 52960937 ID=Msa0506530-mRNA-1:exon:11662;Parent=Msa0506530-mRNA-1
chr3_4 exon 52962283 52962410 52962283 ID=Msa0506530-mRNA-1:exon:11661;Parent=Msa0506530-mRNA-1
chr3_4 exon 52962781 52963149 52962781 ID=Msa0506530-mRNA-1:exon:11660;Parent=Msa0506530-mRNA-1
chr3_4 five_prime_UTR 52962851 52963149 52962851 ID=Msa0506530-mRNA-1:five_prime_utr;Parent=Msa0506530-mRNA-1
chr3_4 CDS 52962781 52962850 52962781 ID=Msa0506530-mRNA-1:cds;Parent=Msa0506530-mRNA-1
chr3_4 CDS 52962283 52962410 52962283 ID=Msa0506530-mRNA-1:cds;Parent=Msa0506530-mRNA-1
chr3_4 CDS 52960937 52961041 52960937 ID=Msa0506530-mRNA-1:cds;Parent=Msa0506530-mRNA-1
chr3_4 CDS 52960168 52960311 52960168 ID=Msa0506530-mRNA-1:cds;Parent=Msa0506530-mRNA-1
chr3_4 three_prime_UTR 52959642 52960167 52959642 ID=Msa0506530-mRNA-1:three_prime_utr;Parent=Msa0506530-mRNA-1
Gene Sequence

>Msa0506530

ATGGCATCGAAACGGATCCTCAAGGAGCTCAAGGATTTGCAGAAGGATCCACCAACATCATGCAGTGCAGGTCCTGTGGCTGAGGACATGTTTCATTGGCAAGCAACAATCATGGGACCTGCTGATAGCCCTTATGCTGGTGGTGTGTTTTTGGTTTCAATTCATTTTCCTCCTGATTATCCATTCAAGCCACCAAAGGTTGCATTTAGGACTAAGGTATTCCACCCAAACATCAACAGCAATGGAAGTATTTGTCTCGACATTCTTAAGGAACAATGGAGCCCTGCACTGACCATTTCCAAGGTGCTTTTGTCAATCTGCTCATTGTTGACGGATCCCAATCCCGATGACCCGCTTGTGCCTGAAATTGCACATATGTACAAGACTGACAGGACCAAGTATGAAGCCACCGCACGCAGCTGGACACAGAAGTATGCCATGGGATGA
Protein sequence

>Msa0506530

MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRTKYEATARSWTQKYAMG*