AlfalfaGEDB | Msa1202000

M. sativa cultivar zhongmu-4 / Msa1202000

KEGG: K10688
GO: -
PFAM: PF00179.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1202000	AET03852.1	99.379	161	1	0	1	161	1	161	1.53e-116	336

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1202000	sp\|Q9FWT2\|UBC16_ARATH	85.714	161	23	0	1	161	1	161	2.03e-98	283

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa1202000	-0.801674	8.055310e-49	-8.615850e-47
Msa0140780	Msa1202000	-0.800667	1.294303e-48	-8.615850e-47
Msa0157290	Msa1202000	-0.801929	7.139811e-49	-8.615850e-47
Msa0215320	Msa1202000	0.801356	9.359293e-49	-8.615850e-47
Msa0367140	Msa1202000	0.815735	7.938067e-52	-8.615850e-47
Msa0459730	Msa1202000	0.818535	1.863445e-52	-8.615850e-47
Msa0506530	Msa1202000	0.881018	3.400863e-70	-8.615850e-47
Msa0719490	Msa1202000	0.805007	1.643117e-49	-8.615850e-47
Msa0769390	Msa1202000	-0.805838	1.100181e-49	-8.615850e-47
Msa0832370	Msa1202000	-0.803314	3.698151e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1202000	MtrunA17_Chr8g0372391	99.379	161	1	0	1	161	1	161	1.41e-120	336
Msa1202000	MtrunA17_Chr5g0404931	88.199	161	19	0	1	161	1	161	3.40e-101	287
Msa1202000	MtrunA17_Chr7g0275361	40.179	112	65	2	15	125	1	111	1.82e-23	90.1
Msa1202000	MtrunA17_Chr8g0369311	40.708	113	65	2	14	125	56	167	4.41e-23	90.5
Msa1202000	MtrunA17_Chr1g0154011	41.071	112	64	2	15	125	1	111	1.35e-22	87.8
Msa1202000	MtrunA17_Chr4g0037011	40.179	112	65	2	15	125	1	111	2.49e-22	87.0
Msa1202000	MtrunA17_Chr3g0108261	39.286	112	66	2	15	125	1	111	3.15e-22	86.7
Msa1202000	MtrunA17_Chr5g0432831	38.393	112	67	2	15	125	1	111	1.34e-21	85.1
Msa1202000	MtrunA17_Chr8g0378601	34.821	112	71	2	16	126	23	133	1.01e-19	80.9
Msa1202000	MtrunA17_Chr8g0362321	37.719	114	69	2	12	124	1	113	2.14e-19	79.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1202000	AT1G75440.1	85.714	161	23	0	1	161	1	161	2.06e-99	283
Msa1202000	AT5G42990.1	86.335	161	22	0	1	161	1	161	2.57e-98	280
Msa1202000	AT1G45050.1	85.714	161	23	0	1	161	1	161	5.85e-98	280
Msa1202000	AT4G36410.1	78.882	161	34	0	1	161	1	161	1.31e-92	266
Msa1202000	AT1G45050.2	79.412	102	21	0	1	102	1	102	3.60e-59	180
Msa1202000	AT3G08690.2	40.179	112	65	2	15	125	1	111	1.05e-22	88.2
Msa1202000	AT3G08690.1	40.179	112	65	2	15	125	1	111	1.05e-22	88.2
Msa1202000	AT5G41700.4	38.938	113	66	2	15	125	1	112	4.96e-22	86.7
Msa1202000	AT5G53300.2	39.286	112	66	2	15	125	1	111	6.61e-22	86.3
Msa1202000	AT5G53300.4	39.286	112	66	2	15	125	1	111	6.61e-22	86.3

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Find 68 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCACCATCTTGTCTCCTT+GGG	0.464987	8_1:+55341829	None:intergenic
AACCTTGCGAGAGGAGTTTG+AGG	0.504445	8_1:+55345684	None:intergenic
AAGAGAGAAAGAGAGCGTAT+CGG	0.605002	8_1:+55345761	None:intergenic
AAGCTCTTTCTGAAGCCTAT+TGG	0.327124	8_1:+55345564	None:intergenic
AAGTCAGCGATAATCTCCAA+AGG	0.650491	8_1:-55345501	Msa1202000:intron
AATTCACAAAACTAGAATAG+AGG	0.513456	8_1:-55341775	Msa1202000:three_prime_UTR
ACAATAGATTCACACCTTAG+CGG	0.657902	8_1:+55342055	None:intergenic
ACACCTGTGGAGCTTCCATT+GGG	0.516280	8_1:+55344529	None:intergenic
ACAGAACTAACAGTCATGGC+AGG	0.555931	8_1:+55342113	None:intergenic
ACATAGCGGTCATTATCTTC+AGG	0.421373	8_1:+55341877	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTAAGGAAGAGATGAAT+TGG	+	chr8_1:55341743-55341762	None:intergenic	30.0%
ACATATATAACCACATGTGT+TGG	-	chr8_1:55342287-55342306	Msa1202000:intron	30.0%
CATTCAAAATTGAGGTTAGA+TGG	+	chr8_1:55342370-55342389	None:intergenic	30.0%
AACAACATTCAAAATCATCG+TGG	-	chr8_1:55342509-55342528	Msa1202000:intron	30.0%
TGTAATAAGCCATTGGATTA+AGG	-	chr8_1:55342842-55342861	Msa1202000:intron	30.0%
AACGAAAATCCTTAATCCAA+TGG	+	chr8_1:55342854-55342873	None:intergenic	30.0%
GTACTGCAACTTGAAATTAA+TGG	-	chr8_1:55343158-55343177	Msa1202000:intron	30.0%
TTGAAATTAATGGGATGAAG+TGG	-	chr8_1:55343168-55343187	Msa1202000:intron	30.0%
AGAAAGAGACAAAGTGAAAA+AGG	+	chr8_1:55343392-55343411	None:intergenic	30.0%
TTGTATTCGTTAGCGTTTAT+TGG	-	chr8_1:55343447-55343466	Msa1202000:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	55341559	55345812	55341559	ID=Msa1202000;Name=Msa1202000
chr8_1	mRNA	55341559	55345812	55341559	ID=Msa1202000-mRNA-1;Parent=Msa1202000;Name=Msa1202000-mRNA-1;_AED=0.09;_eAED=0.09;_QI=96\|0.8\|0.83\|1\|1\|1\|6\|251\|161
chr8_1	exon	55341559	55341905	55341559	ID=Msa1202000-mRNA-1:exon:17929;Parent=Msa1202000-mRNA-1
chr8_1	exon	55342070	55342158	55342070	ID=Msa1202000-mRNA-1:exon:17928;Parent=Msa1202000-mRNA-1
chr8_1	exon	55344392	55344455	55344392	ID=Msa1202000-mRNA-1:exon:17927;Parent=Msa1202000-mRNA-1
chr8_1	exon	55344533	55344635	55344533	ID=Msa1202000-mRNA-1:exon:17926;Parent=Msa1202000-mRNA-1
chr8_1	exon	55345502	55345605	55345502	ID=Msa1202000-mRNA-1:exon:17925;Parent=Msa1202000-mRNA-1
chr8_1	exon	55345687	55345812	55345687	ID=Msa1202000-mRNA-1:exon:17924;Parent=Msa1202000-mRNA-1
chr8_1	five_prime_UTR	55345717	55345812	55345717	ID=Msa1202000-mRNA-1:five_prime_utr;Parent=Msa1202000-mRNA-1
chr8_1	CDS	55345687	55345716	55345687	ID=Msa1202000-mRNA-1:cds;Parent=Msa1202000-mRNA-1
chr8_1	CDS	55345502	55345605	55345502	ID=Msa1202000-mRNA-1:cds;Parent=Msa1202000-mRNA-1
chr8_1	CDS	55344533	55344635	55344533	ID=Msa1202000-mRNA-1:cds;Parent=Msa1202000-mRNA-1
chr8_1	CDS	55344392	55344455	55344392	ID=Msa1202000-mRNA-1:cds;Parent=Msa1202000-mRNA-1
chr8_1	CDS	55342070	55342158	55342070	ID=Msa1202000-mRNA-1:cds;Parent=Msa1202000-mRNA-1
chr8_1	CDS	55341810	55341905	55341810	ID=Msa1202000-mRNA-1:cds;Parent=Msa1202000-mRNA-1
chr8_1	three_prime_UTR	55341559	55341809	55341559	ID=Msa1202000-mRNA-1:three_prime_utr;Parent=Msa1202000-mRNA-1

Gene Sequence

>Msa1202000

ATGACTAGCTCCTCAAACTCCTCTCGCAAGAATCTCAGTAAGATTGCCTCCAATAGGCTTCAGAAAGAGCTTGTTGAATGGCAGATTAATCCTCCTGCTGGTTTCAACTACAAAGTCAGCGATAATCTCCAAAGGTGGGTTATTGAAGTGATTGGAGCTCCTGGGACACTTTATGCTAATGAGACCTACAAGCTTCAGGTCGATTTCCCAGAGCACTACCCAATGGAAGCTCCACAGGTCATATTTATGAATCCGGCCCCAATGCATCCCCATATCTATAGCAACGGGCATATTTGTTTAGATATATTGTATGATTCTTGGTCCCCTGCCATGACTGTTAGTTCTGTATGCATCAGCATTCTCTCAATGCTCTCAAGCTCACCCGCTAAGCAACGGCCTGAAGATAATGACCGCTATGTAAAGAACTGTAGAAATGGAAGATCTCCCAAGGAGACAAGATGGTGGTTCCATGATGATAAAGTATAA

Protein sequence

>Msa1202000

MTSSSNSSRKNLSKIASNRLQKELVEWQINPPAGFNYKVSDNLQRWVIEVIGAPGTLYANETYKLQVDFPEHYPMEAPQVIFMNPAPMHPHIYSNGHICLDILYDSWSPAMTVSSVCISILSMLSSSPAKQRPEDNDRYVKNCRNGRSPKETRWWFHDDKV*