AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0471000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471000 MtrunA17_Chr3g0125791 96.825 126 4 0 1 126 1 126 4.39e-89 254
Msa0471000 MtrunA17_Chr3g0125761 66.400 125 42 0 1 125 1 125 4.13e-61 183
Msa0471000 MtrunA17_Chr8g0380901 62.698 126 40 2 1 125 1 120 9.11e-55 167
Msa0471000 MtrunA17_Chr2g0301431 47.619 126 63 2 1 125 1 124 1.21e-39 129
Msa0471000 MtrunA17_Chr8g0380711 55.882 102 44 1 25 125 20 121 3.51e-38 125
Msa0471000 MtrunA17_Chr5g0394321 37.607 117 64 3 14 123 7 121 3.00e-22 86.7
Msa0471000 MtrunA17_Chr8g0335531 40.984 122 66 4 10 126 1 121 1.63e-21 84.7
Msa0471000 MtrunA17_Chr1g0205701 37.168 113 62 3 18 123 10 120 1.27e-20 82.4
Msa0471000 MtrunA17_Chr8g0335571 39.344 122 68 4 10 126 1 121 1.70e-19 79.7
Msa0471000 MtrunA17_Chr1g0177341 35.780 109 66 3 19 123 11 119 5.64e-19 77.4
Msa0471000 MtrunA17_Chr1g0177331 35.780 109 66 3 19 123 11 119 5.64e-19 77.4
Msa0471000 MtrunA17_Chr7g0253681 34.167 120 75 3 8 123 2 121 1.54e-18 76.3
Msa0471000 MtrunA17_Chr7g0253731 32.143 112 72 3 9 117 2 112 1.93e-18 76.3
Msa0471000 MtrunA17_Chr4g0064041 39.394 99 53 2 32 123 190 288 4.72e-18 78.2
Msa0471000 MtrunA17_Chr4g0064041 34.351 131 68 4 12 126 1 129 9.31e-16 72.0
Msa0471000 MtrunA17_Chr7g0253701 34.167 120 74 4 8 123 2 120 1.05e-17 74.3
Msa0471000 MtrunA17_Chr7g0253671 34.167 120 74 4 8 123 2 120 1.05e-17 74.3
Msa0471000 MtrunA17_Chr3g0132441 38.393 112 65 3 15 123 8 118 2.52e-17 73.9
Msa0471000 MtrunA17_Chr2g0306461 35.484 124 69 4 10 123 1 123 3.69e-17 73.6
Msa0471000 MtrunA17_Chr7g0253761 34.454 119 73 3 10 123 1 119 6.01e-17 72.8
Msa0471000 MtrunA17_Chr1g0211101 34.483 116 71 4 12 123 13 127 1.16e-16 72.0
Msa0471000 MtrunA17_Chr2g0323021 35.897 117 68 5 11 123 5 118 1.16e-16 72.8
Msa0471000 MtrunA17_Chr7g0253641 35.185 108 67 2 19 123 12 119 1.47e-16 72.8
Msa0471000 MtrunA17_Chr3g0132701 38.053 113 66 3 15 123 8 120 1.48e-16 72.4
Msa0471000 MtrunA17_Chr6g0459441 40.816 98 53 4 30 123 23 119 3.73e-16 70.5
Msa0471000 MtrunA17_Chr4g0033131 31.405 121 70 3 16 123 7 127 1.84e-15 68.6
Msa0471000 MtrunA17_Chr4g0033531 31.405 121 70 3 16 123 7 127 1.84e-15 68.6
Msa0471000 MtrunA17_Chr3g0132511 37.168 113 67 3 15 123 8 120 1.91e-15 69.7
Msa0471000 MtrunA17_Chr6g0483981 33.333 120 71 2 15 125 9 128 2.83e-15 70.9
Msa0471000 MtrunA17_Chr3g0132431 37.500 112 66 3 15 123 8 118 5.25e-15 67.8
Msa0471000 MtrunA17_Chr7g0253631 34.454 119 73 3 10 123 1 119 1.30e-14 67.8
Msa0471000 MtrunA17_Chr6g0453801 32.520 123 79 2 7 125 9 131 1.39e-14 66.2
Msa0471000 MtrunA17_Chr6g0453921 31.579 133 81 3 3 125 2 134 1.96e-14 66.2
Msa0471000 MtrunA17_Chr6g0481491 30.769 104 68 3 23 123 18 120 4.81e-14 65.5
Msa0471000 MtrunA17_Chr4g0001571 32.653 98 64 1 30 125 26 123 4.16e-13 62.4
Msa0471000 MtrunA17_Chr4g0041811 28.800 125 82 2 9 126 8 132 5.90e-13 62.8
Msa0471000 MtrunA17_Chr8g0387804 27.350 117 79 2 13 123 9 125 4.56e-12 58.9
Msa0471000 MtrunA17_Chr5g0440711 30.328 122 80 3 7 123 3 124 2.05e-11 57.4
Msa0471000 MtrunA17_Chr6g0453791 28.455 123 84 3 5 123 2 124 3.73e-11 57.4
Msa0471000 MtrunA17_Chr4g0064031 33.019 106 59 4 26 120 21 125 7.24e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471000 AT2G02850.1 54.264 129 56 1 1 126 1 129 1.32e-47 149
Msa0471000 AT1G17800.1 38.095 126 72 3 7 126 12 137 1.45e-26 96.7
Msa0471000 AT2G32300.1 37.705 122 72 3 8 126 2 122 4.58e-23 90.9
Msa0471000 AT2G32300.2 37.705 122 72 3 8 126 32 152 7.10e-23 90.9
Msa0471000 AT5G26330.1 34.426 122 75 2 10 126 1 122 1.60e-22 87.8
Msa0471000 AT3G17675.1 35.246 122 76 2 5 123 4 125 1.41e-21 83.6
Msa0471000 AT5G07475.1 38.739 111 61 4 19 123 13 122 5.15e-20 81.3
Msa0471000 AT3G60280.1 41.237 97 53 3 30 123 21 116 1.40e-19 80.9
Msa0471000 AT3G27200.1 36.364 121 72 4 10 126 3 122 2.51e-19 79.0
Msa0471000 AT2G44790.1 40.741 108 54 5 24 126 23 125 1.47e-17 75.1
Msa0471000 AT3G60270.1 37.113 97 57 3 30 123 23 118 4.35e-17 73.6
Msa0471000 AT2G25060.1 32.773 119 72 3 16 126 14 132 1.98e-15 69.3
Msa0471000 AT2G31050.1 33.043 115 72 3 17 126 12 126 2.21e-15 69.3
Msa0471000 AT2G26720.1 30.579 121 79 3 11 126 6 126 3.91e-15 68.9
Msa0471000 AT4G31840.1 29.134 127 82 3 8 126 2 128 6.87e-15 67.8
Msa0471000 AT1G72230.1 35.345 116 69 4 11 123 3 115 2.08e-13 63.9
Msa0471000 AT3G20570.1 30.952 126 80 3 4 123 2 126 2.90e-13 63.9
Msa0471000 AT5G20230.1 34.000 100 60 2 33 126 25 124 9.35e-13 62.4
Msa0471000 AT2G27035.1 32.500 120 60 6 16 123 12 122 4.43e-12 60.1
Msa0471000 AT4G30590.1 27.907 129 81 4 10 126 1 129 1.39e-11 59.3
Msa0471000 AT1G22480.1 37.069 116 64 5 12 123 1 111 2.24e-11 58.2
Msa0471000 AT5G15350.1 29.060 117 78 3 12 123 7 123 5.75e-11 57.4

Find 69 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTCATGCTGTTATTTATA+TGG 0.243835 3_3:+73204633 None:intergenic
AAGATCCCTTTGTTTGTTTA+CGG 0.244966 3_3:-73204439 Msa0471000:three_prime_UTR
TGAAAACTTGAAATTATTTA+CGG 0.262358 3_3:+73204379 None:intergenic
TTTGGTGATCAATATGTAAA+AGG 0.277801 3_3:-73204587 Msa0471000:three_prime_UTR
AGACTTATCCCTTTATATAA+TGG 0.283252 3_3:+73205370 None:intergenic
GTAGTTAATTAGTTAATTAA+TGG 0.284468 3_3:-73204690 Msa0471000:three_prime_UTR
AATTTCACTGCTGTGCCTTT+TGG 0.295835 3_3:-73205223 Msa0471000:CDS
CTACTAGCTATATGTTGATT+TGG 0.303864 3_3:-73204605 Msa0471000:three_prime_UTR
AATTAATAAAACTCCCGTTT+TGG 0.313421 3_3:-73204344 Msa0471000:three_prime_UTR
TCATCAATTCCATTATATAA+AGG 0.321055 3_3:-73205379 Msa0471000:five_prime_UTR
TATCCTGGTCACTGCCAATC+TGG 0.329505 3_3:-73204767 Msa0471000:CDS
GTCCTCTTGATAGCCTGATT+TGG 0.349417 3_3:+73204810 None:intergenic
CATCAATTCCATTATATAAA+GGG 0.362055 3_3:-73205378 Msa0471000:five_prime_UTR
AAAACTGATACTTGGCCTAA+TGG 0.367231 3_3:-73205138 Msa0471000:CDS
GGTGCTAAAGTGTTCAGTTC+AGG 0.375248 3_3:-73204839 Msa0471000:CDS
CATATTCTCACTATTTCTCT+TGG 0.410921 3_3:+73205287 None:intergenic
AGAGGACTAAACTGCATAGA+TGG 0.414690 3_3:+73204733 None:intergenic
AAAATCTGAGGGACCAAAAC+GGG 0.432586 3_3:+73204331 None:intergenic
TCTTACACCGTTGGAGGACC+TGG 0.442628 3_3:-73205174 Msa0471000:CDS
GTTCAGGGAATGACCAAATC+AGG 0.451771 3_3:-73204823 Msa0471000:CDS
TTCTAGTGAGATGGTTGAGT+TGG 0.453959 3_3:+73205335 None:intergenic
TAAATAACAGCATGAGTACT+AGG 0.471252 3_3:-73204628 Msa0471000:three_prime_UTR
GATGGTTGAGTTGGTTTGCA+AGG 0.474704 3_3:+73205344 None:intergenic
TACTTCATATGCAGCTATCC+TGG 0.480047 3_3:-73204782 Msa0471000:CDS
GAGAATATGACTGAGGGAAG+AGG 0.490305 3_3:-73205273 Msa0471000:exon
ATCCTGGTCACTGCCAATCT+GGG 0.503016 3_3:-73204766 Msa0471000:CDS
TGATGAATCTGTTGAGCTAT+GGG 0.503893 3_3:+73205396 None:intergenic
CACTTTCAGCCAGAACCAAA+AGG 0.511460 3_3:+73205208 None:intergenic
GTGCTAAAGTGTTCAGTTCA+GGG 0.512300 3_3:-73204838 Msa0471000:CDS
TTGATGAATCTGTTGAGCTA+TGG 0.522374 3_3:+73205395 None:intergenic
ATCCCAGATTGGCAGTGACC+AGG 0.530524 3_3:+73204764 None:intergenic
GAAAATCTGAGGGACCAAAA+CGG 0.530828 3_3:+73204330 None:intergenic
TAATGGTCAATTAGCTGTAG+TGG 0.537805 3_3:-73204673 Msa0471000:three_prime_UTR
GGACATACAAAACTGATACT+TGG 0.540585 3_3:-73205146 Msa0471000:CDS
TGGAAACCTTCATCCCAGAT+TGG 0.543764 3_3:+73204753 None:intergenic
TCTCACAAGCAGGGATAGGT+TGG 0.544854 3_3:+73205460 None:intergenic
CACAAGCAGGGATAGGTTGG+TGG 0.545157 3_3:+73205463 None:intergenic
TCACTGCCAATCTGGGATGA+AGG 0.545313 3_3:-73204759 Msa0471000:CDS
CATCTCACTAGAATTGCTCT+AGG 0.545816 3_3:-73205325 Msa0471000:five_prime_UTR
TGGATCTCACAAGCAGGGAT+AGG 0.547940 3_3:+73205456 None:intergenic
CTTAGACCGTAAACAAACAA+AGG 0.558115 3_3:+73204433 None:intergenic
TTAGACCGTAAACAAACAAA+GGG 0.559350 3_3:+73204434 None:intergenic
CTAGAGCAATTCTAGTGAGA+TGG 0.561240 3_3:+73205326 None:intergenic
TTGGATGCTACTCACTAGCT+AGG 0.563622 3_3:+73205550 None:intergenic
GCAGCATCTTACACCGTTGG+AGG 0.574303 3_3:-73205180 Msa0471000:CDS
GTGGTTGCCGTGGACAAAAG+TGG 0.576000 3_3:-73204887 Msa0471000:CDS
CTGAACACTTTAGCACCTCC+CGG 0.578857 3_3:+73204845 None:intergenic
AATGCAGCATCTTACACCGT+TGG 0.578987 3_3:-73205183 Msa0471000:CDS
CACACACAATGTGGTTGCCG+TGG 0.585202 3_3:-73204897 Msa0471000:CDS
AATAGTGAGAATATGACTGA+GGG 0.586297 3_3:-73205279 Msa0471000:exon
CTTACACCGTTGGAGGACCT+GGG 0.588429 3_3:-73205173 Msa0471000:CDS
AAATAGTGAGAATATGACTG+AGG 0.592419 3_3:-73205280 Msa0471000:exon
ATCAATATGTAAAAGGAGTG+TGG 0.597954 3_3:-73204580 Msa0471000:three_prime_UTR
ATATTGTGGATCTCACAAGC+AGG 0.598727 3_3:+73205450 None:intergenic
AAGGGAGAGGATGGTAATGT+CGG 0.599256 3_3:-73205575 Msa0471000:five_prime_UTR
TAGTCAAGTCACATAGCTAG+AGG 0.624989 3_3:+73204715 None:intergenic
TACAACAACTGTAAGACACC+GGG 0.625426 3_3:-73204863 Msa0471000:CDS
TTACACCGTTGGAGGACCTG+GGG 0.632787 3_3:-73205172 Msa0471000:CDS
CACGGCAACCACATTGTGTG+TGG 0.635175 3_3:+73204898 None:intergenic
TATTGTGGATCTCACAAGCA+GGG 0.635490 3_3:+73205451 None:intergenic
GACCAAATCAGGCTATCAAG+AGG 0.635565 3_3:-73204812 Msa0471000:CDS
AGGCAGTGCATCTATGAACA+TGG 0.647845 3_3:-73205253 Msa0471000:CDS
ATTGTGTAGATTGAATATTG+TGG 0.652337 3_3:+73205436 None:intergenic
TGATTCAACCACACACAATG+TGG 0.661122 3_3:-73204906 Msa0471000:CDS
ATACAACAACTGTAAGACAC+CGG 0.661265 3_3:-73204864 Msa0471000:CDS
TACACCGTTGGAGGACCTGG+GGG 0.694377 3_3:-73205171 Msa0471000:CDS
TATGTAAAAGGAGTGTGGTG+AGG 0.701233 3_3:-73204575 Msa0471000:three_prime_UTR
AACAACTGTAAGACACCGGG+AGG 0.711932 3_3:-73204860 Msa0471000:CDS
GATGAATCTGTTGAGCTATG+GGG 0.737724 3_3:+73205397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTAATTACAGTACAAT+TGG - chr3_3:73204875-73204894 Msa0471000:CDS 15.0%
!! AATTTAATTACAGTACAATT+GGG - chr3_3:73204876-73204895 Msa0471000:CDS 15.0%
!!! AAAATTGAGAAATATAGTTT+TGG + chr3_3:73204915-73204934 None:intergenic 15.0%
!!! TCAATTTTACAAAATTGTTT+TGG - chr3_3:73204927-73204946 Msa0471000:intron 15.0%
!! GTAGTTAATTAGTTAATTAA+TGG - chr3_3:73205231-73205250 Msa0471000:CDS 15.0%
!!! TTTTGGTTTATTTAATTCAT+TGG - chr3_3:73205517-73205536 Msa0471000:five_prime_UTR 15.0%
!! TGAAAACTTGAAATTATTTA+CGG + chr3_3:73205545-73205564 None:intergenic 15.0%
!! TAATTTAATTACAGTACAAT+TGG - chr3_3:73204875-73204894 Msa0471000:CDS 15.0%
!! AATTTAATTACAGTACAATT+GGG - chr3_3:73204876-73204895 Msa0471000:CDS 15.0%
!!! AAAATTGAGAAATATAGTTT+TGG + chr3_3:73204915-73204934 None:intergenic 15.0%
!!! TCAATTTTACAAAATTGTTT+TGG - chr3_3:73204927-73204946 Msa0471000:intron 15.0%
!! GTAGTTAATTAGTTAATTAA+TGG - chr3_3:73205231-73205250 Msa0471000:CDS 15.0%
!!! TTTTGGTTTATTTAATTCAT+TGG - chr3_3:73205517-73205536 Msa0471000:five_prime_UTR 15.0%
!! TGAAAACTTGAAATTATTTA+CGG + chr3_3:73205545-73205564 None:intergenic 15.0%
!! TCATCAATTCCATTATATAA+AGG - chr3_3:73204542-73204561 Msa0471000:three_prime_UTR 20.0%
!! CATCAATTCCATTATATAAA+GGG - chr3_3:73204543-73204562 Msa0471000:three_prime_UTR 20.0%
!! ACTTTAATTATATGTGCATA+TGG - chr3_3:73204966-73204985 Msa0471000:intron 20.0%
!! TCATCAATTCCATTATATAA+AGG - chr3_3:73204542-73204561 Msa0471000:three_prime_UTR 20.0%
!! CATCAATTCCATTATATAAA+GGG - chr3_3:73204543-73204562 Msa0471000:three_prime_UTR 20.0%
!! ACTTTAATTATATGTGCATA+TGG - chr3_3:73204966-73204985 Msa0471000:intron 20.0%
! ATTGTGTAGATTGAATATTG+TGG + chr3_3:73204488-73204507 None:intergenic 25.0%
! AGACTTATCCCTTTATATAA+TGG + chr3_3:73204554-73204573 None:intergenic 25.0%
!!! GCTTTAAACTTTTTTCCATT+AGG + chr3_3:73204801-73204820 None:intergenic 25.0%
!! AATGGAAAAAAGTTTAAAGC+TGG - chr3_3:73204801-73204820 Msa0471000:CDS 25.0%
!! TACTCATGCTGTTATTTATA+TGG + chr3_3:73205291-73205310 None:intergenic 25.0%
!! TTTGGTGATCAATATGTAAA+AGG - chr3_3:73205334-73205353 Msa0471000:five_prime_UTR 25.0%
!!! GTTTGATATGTTTGTTTTTG+TGG - chr3_3:73205392-73205411 Msa0471000:five_prime_UTR 25.0%
!!! TACGGTCTAAGTTTTTATTT+TGG - chr3_3:73205500-73205519 Msa0471000:five_prime_UTR 25.0%
!! AATTAATAAAACTCCCGTTT+TGG - chr3_3:73205577-73205596 Msa0471000:five_prime_UTR 25.0%
! ATTGTGTAGATTGAATATTG+TGG + chr3_3:73204488-73204507 None:intergenic 25.0%
! AGACTTATCCCTTTATATAA+TGG + chr3_3:73204554-73204573 None:intergenic 25.0%
!!! GCTTTAAACTTTTTTCCATT+AGG + chr3_3:73204801-73204820 None:intergenic 25.0%
!! AATGGAAAAAAGTTTAAAGC+TGG - chr3_3:73204801-73204820 Msa0471000:CDS 25.0%
!! TACTCATGCTGTTATTTATA+TGG + chr3_3:73205291-73205310 None:intergenic 25.0%
!! TTTGGTGATCAATATGTAAA+AGG - chr3_3:73205334-73205353 Msa0471000:five_prime_UTR 25.0%
!!! GTTTGATATGTTTGTTTTTG+TGG - chr3_3:73205392-73205411 Msa0471000:five_prime_UTR 25.0%
!!! TACGGTCTAAGTTTTTATTT+TGG - chr3_3:73205500-73205519 Msa0471000:five_prime_UTR 25.0%
!! AATTAATAAAACTCCCGTTT+TGG - chr3_3:73205577-73205596 Msa0471000:five_prime_UTR 25.0%
! TGGCTTTTCTTCAATAGTTA+AGG + chr3_3:73204441-73204460 None:intergenic 30.0%
! CATATTCTCACTATTTCTCT+TGG + chr3_3:73204637-73204656 None:intergenic 30.0%
AAATAGTGAGAATATGACTG+AGG - chr3_3:73204641-73204660 Msa0471000:three_prime_UTR 30.0%
AATAGTGAGAATATGACTGA+GGG - chr3_3:73204642-73204661 Msa0471000:three_prime_UTR 30.0%
ATATGGTAATTGATTGTTGC+AGG - chr3_3:73204983-73205002 Msa0471000:intron 30.0%
TAAATAACAGCATGAGTACT+AGG - chr3_3:73205293-73205312 Msa0471000:five_prime_UTR 30.0%
! CTACTAGCTATATGTTGATT+TGG - chr3_3:73205316-73205335 Msa0471000:five_prime_UTR 30.0%
ATCAATATGTAAAAGGAGTG+TGG - chr3_3:73205341-73205360 Msa0471000:five_prime_UTR 30.0%
! AAGATCCCTTTGTTTGTTTA+CGG - chr3_3:73205482-73205501 Msa0471000:five_prime_UTR 30.0%
TTAGACCGTAAACAAACAAA+GGG + chr3_3:73205490-73205509 None:intergenic 30.0%
!!! ACGGGAGTTTTATTAATTGT+GGG + chr3_3:73205575-73205594 None:intergenic 30.0%
!!! AACGGGAGTTTTATTAATTG+TGG + chr3_3:73205576-73205595 None:intergenic 30.0%
! TGGCTTTTCTTCAATAGTTA+AGG + chr3_3:73204441-73204460 None:intergenic 30.0%
! CATATTCTCACTATTTCTCT+TGG + chr3_3:73204637-73204656 None:intergenic 30.0%
AAATAGTGAGAATATGACTG+AGG - chr3_3:73204641-73204660 Msa0471000:three_prime_UTR 30.0%
AATAGTGAGAATATGACTGA+GGG - chr3_3:73204642-73204661 Msa0471000:three_prime_UTR 30.0%
ATATGGTAATTGATTGTTGC+AGG - chr3_3:73204983-73205002 Msa0471000:intron 30.0%
TAAATAACAGCATGAGTACT+AGG - chr3_3:73205293-73205312 Msa0471000:five_prime_UTR 30.0%
! CTACTAGCTATATGTTGATT+TGG - chr3_3:73205316-73205335 Msa0471000:five_prime_UTR 30.0%
ATCAATATGTAAAAGGAGTG+TGG - chr3_3:73205341-73205360 Msa0471000:five_prime_UTR 30.0%
! AAGATCCCTTTGTTTGTTTA+CGG - chr3_3:73205482-73205501 Msa0471000:five_prime_UTR 30.0%
TTAGACCGTAAACAAACAAA+GGG + chr3_3:73205490-73205509 None:intergenic 30.0%
!!! ACGGGAGTTTTATTAATTGT+GGG + chr3_3:73205575-73205594 None:intergenic 30.0%
!!! AACGGGAGTTTTATTAATTG+TGG + chr3_3:73205576-73205595 None:intergenic 30.0%
TGATGAATCTGTTGAGCTAT+GGG + chr3_3:73204528-73204547 None:intergenic 35.0%
TTGATGAATCTGTTGAGCTA+TGG + chr3_3:73204529-73204548 None:intergenic 35.0%
GGACATACAAAACTGATACT+TGG - chr3_3:73204775-73204794 Msa0471000:CDS 35.0%
AAAACTGATACTTGGCCTAA+TGG - chr3_3:73204783-73204802 Msa0471000:CDS 35.0%
ATACAACAACTGTAAGACAC+CGG - chr3_3:73205057-73205076 Msa0471000:intron 35.0%
TAATGGTCAATTAGCTGTAG+TGG - chr3_3:73205248-73205267 Msa0471000:CDS 35.0%
CTTAGACCGTAAACAAACAA+AGG + chr3_3:73205491-73205510 None:intergenic 35.0%
TGATGAATCTGTTGAGCTAT+GGG + chr3_3:73204528-73204547 None:intergenic 35.0%
TTGATGAATCTGTTGAGCTA+TGG + chr3_3:73204529-73204548 None:intergenic 35.0%
GGACATACAAAACTGATACT+TGG - chr3_3:73204775-73204794 Msa0471000:CDS 35.0%
AAAACTGATACTTGGCCTAA+TGG - chr3_3:73204783-73204802 Msa0471000:CDS 35.0%
ATACAACAACTGTAAGACAC+CGG - chr3_3:73205057-73205076 Msa0471000:intron 35.0%
TAATGGTCAATTAGCTGTAG+TGG - chr3_3:73205248-73205267 Msa0471000:CDS 35.0%
CTTAGACCGTAAACAAACAA+AGG + chr3_3:73205491-73205510 None:intergenic 35.0%
TATTGTGGATCTCACAAGCA+GGG + chr3_3:73204473-73204492 None:intergenic 40.0%
ATATTGTGGATCTCACAAGC+AGG + chr3_3:73204474-73204493 None:intergenic 40.0%
GATGAATCTGTTGAGCTATG+GGG + chr3_3:73204527-73204546 None:intergenic 40.0%
TTCTAGTGAGATGGTTGAGT+TGG + chr3_3:73204589-73204608 None:intergenic 40.0%
CTAGAGCAATTCTAGTGAGA+TGG + chr3_3:73204598-73204617 None:intergenic 40.0%
CATCTCACTAGAATTGCTCT+AGG - chr3_3:73204596-73204615 Msa0471000:three_prime_UTR 40.0%
! AATTTCACTGCTGTGCCTTT+TGG - chr3_3:73204698-73204717 Msa0471000:three_prime_UTR 40.0%
TGATTCAACCACACACAATG+TGG - chr3_3:73205015-73205034 Msa0471000:intron 40.0%
!! GTTGTATCCACTTTTGTCCA+CGG + chr3_3:73205044-73205063 None:intergenic 40.0%
TACAACAACTGTAAGACACC+GGG - chr3_3:73205058-73205077 Msa0471000:intron 40.0%
! GTGCTAAAGTGTTCAGTTCA+GGG - chr3_3:73205083-73205102 Msa0471000:intron 40.0%
TACTTCATATGCAGCTATCC+TGG - chr3_3:73205139-73205158 Msa0471000:CDS 40.0%
AGAGGACTAAACTGCATAGA+TGG + chr3_3:73205191-73205210 None:intergenic 40.0%
TAGTCAAGTCACATAGCTAG+AGG + chr3_3:73205209-73205228 None:intergenic 40.0%
! TATGTAAAAGGAGTGTGGTG+AGG - chr3_3:73205346-73205365 Msa0471000:five_prime_UTR 40.0%
!!! CCATCCTCTCCCTTTTTTTT+GGG + chr3_3:73204340-73204359 None:intergenic 40.0%
!!! ACCATCCTCTCCCTTTTTTT+TGG + chr3_3:73204341-73204360 None:intergenic 40.0%
TATTGTGGATCTCACAAGCA+GGG + chr3_3:73204473-73204492 None:intergenic 40.0%
ATATTGTGGATCTCACAAGC+AGG + chr3_3:73204474-73204493 None:intergenic 40.0%
GATGAATCTGTTGAGCTATG+GGG + chr3_3:73204527-73204546 None:intergenic 40.0%
TTCTAGTGAGATGGTTGAGT+TGG + chr3_3:73204589-73204608 None:intergenic 40.0%
CTAGAGCAATTCTAGTGAGA+TGG + chr3_3:73204598-73204617 None:intergenic 40.0%
CATCTCACTAGAATTGCTCT+AGG - chr3_3:73204596-73204615 Msa0471000:three_prime_UTR 40.0%
! AATTTCACTGCTGTGCCTTT+TGG - chr3_3:73204698-73204717 Msa0471000:three_prime_UTR 40.0%
TGATTCAACCACACACAATG+TGG - chr3_3:73205015-73205034 Msa0471000:intron 40.0%
!! GTTGTATCCACTTTTGTCCA+CGG + chr3_3:73205044-73205063 None:intergenic 40.0%
TACAACAACTGTAAGACACC+GGG - chr3_3:73205058-73205077 Msa0471000:intron 40.0%
! GTGCTAAAGTGTTCAGTTCA+GGG - chr3_3:73205083-73205102 Msa0471000:intron 40.0%
TACTTCATATGCAGCTATCC+TGG - chr3_3:73205139-73205158 Msa0471000:CDS 40.0%
AGAGGACTAAACTGCATAGA+TGG + chr3_3:73205191-73205210 None:intergenic 40.0%
TAGTCAAGTCACATAGCTAG+AGG + chr3_3:73205209-73205228 None:intergenic 40.0%
! TATGTAAAAGGAGTGTGGTG+AGG - chr3_3:73205346-73205365 Msa0471000:five_prime_UTR 40.0%
! AAGGGAGAGGATGGTAATGT+CGG - chr3_3:73204346-73204365 Msa0471000:three_prime_UTR 45.0%
TTGGATGCTACTCACTAGCT+AGG + chr3_3:73204374-73204393 None:intergenic 45.0%
!! GATGGTTGAGTTGGTTTGCA+AGG + chr3_3:73204580-73204599 None:intergenic 45.0%
GAGAATATGACTGAGGGAAG+AGG - chr3_3:73204648-73204667 Msa0471000:three_prime_UTR 45.0%
AGGCAGTGCATCTATGAACA+TGG - chr3_3:73204668-73204687 Msa0471000:three_prime_UTR 45.0%
CACTTTCAGCCAGAACCAAA+AGG + chr3_3:73204716-73204735 None:intergenic 45.0%
AATGCAGCATCTTACACCGT+TGG - chr3_3:73204738-73204757 Msa0471000:exon 45.0%
! GGTGCTAAAGTGTTCAGTTC+AGG - chr3_3:73205082-73205101 Msa0471000:intron 45.0%
GTTCAGGGAATGACCAAATC+AGG - chr3_3:73205098-73205117 Msa0471000:intron 45.0%
GACCAAATCAGGCTATCAAG+AGG - chr3_3:73205109-73205128 Msa0471000:CDS 45.0%
! GTCCTCTTGATAGCCTGATT+TGG + chr3_3:73205114-73205133 None:intergenic 45.0%
TGGAAACCTTCATCCCAGAT+TGG + chr3_3:73205171-73205190 None:intergenic 45.0%
! TTTTTGTGGTGTGCTGTGTG+AGG - chr3_3:73205406-73205425 Msa0471000:five_prime_UTR 45.0%
! AAGGGAGAGGATGGTAATGT+CGG - chr3_3:73204346-73204365 Msa0471000:three_prime_UTR 45.0%
TTGGATGCTACTCACTAGCT+AGG + chr3_3:73204374-73204393 None:intergenic 45.0%
!! GATGGTTGAGTTGGTTTGCA+AGG + chr3_3:73204580-73204599 None:intergenic 45.0%
GAGAATATGACTGAGGGAAG+AGG - chr3_3:73204648-73204667 Msa0471000:three_prime_UTR 45.0%
AGGCAGTGCATCTATGAACA+TGG - chr3_3:73204668-73204687 Msa0471000:three_prime_UTR 45.0%
CACTTTCAGCCAGAACCAAA+AGG + chr3_3:73204716-73204735 None:intergenic 45.0%
AATGCAGCATCTTACACCGT+TGG - chr3_3:73204738-73204757 Msa0471000:exon 45.0%
! GGTGCTAAAGTGTTCAGTTC+AGG - chr3_3:73205082-73205101 Msa0471000:intron 45.0%
GTTCAGGGAATGACCAAATC+AGG - chr3_3:73205098-73205117 Msa0471000:intron 45.0%
GACCAAATCAGGCTATCAAG+AGG - chr3_3:73205109-73205128 Msa0471000:CDS 45.0%
! GTCCTCTTGATAGCCTGATT+TGG + chr3_3:73205114-73205133 None:intergenic 45.0%
TGGAAACCTTCATCCCAGAT+TGG + chr3_3:73205171-73205190 None:intergenic 45.0%
! TTTTTGTGGTGTGCTGTGTG+AGG - chr3_3:73205406-73205425 Msa0471000:five_prime_UTR 45.0%
!!! TTTTTTTTTTTTTTTAAAGT+TGG + chr3_3:73204393-73204412 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTAAAGT+TGG + chr3_3:73204393-73204412 None:intergenic 5.0%
TCTCACAAGCAGGGATAGGT+TGG + chr3_3:73204464-73204483 None:intergenic 50.0%
TGGATCTCACAAGCAGGGAT+AGG + chr3_3:73204468-73204487 None:intergenic 50.0%
!!! ACTGCTGTGCCTTTTGGTTC+TGG - chr3_3:73204704-73204723 Msa0471000:three_prime_UTR 50.0%
! GTTTTGTATGTCCATCCCCC+AGG + chr3_3:73204768-73204787 None:intergenic 50.0%
AACAACTGTAAGACACCGGG+AGG - chr3_3:73205061-73205080 Msa0471000:intron 50.0%
CTGAACACTTTAGCACCTCC+CGG + chr3_3:73205079-73205098 None:intergenic 50.0%
TATCCTGGTCACTGCCAATC+TGG - chr3_3:73205154-73205173 Msa0471000:CDS 50.0%
ATCCTGGTCACTGCCAATCT+GGG - chr3_3:73205155-73205174 Msa0471000:CDS 50.0%
! TCACTGCCAATCTGGGATGA+AGG - chr3_3:73205162-73205181 Msa0471000:CDS 50.0%
TCTCACAAGCAGGGATAGGT+TGG + chr3_3:73204464-73204483 None:intergenic 50.0%
TGGATCTCACAAGCAGGGAT+AGG + chr3_3:73204468-73204487 None:intergenic 50.0%
!!! ACTGCTGTGCCTTTTGGTTC+TGG - chr3_3:73204704-73204723 Msa0471000:three_prime_UTR 50.0%
! GTTTTGTATGTCCATCCCCC+AGG + chr3_3:73204768-73204787 None:intergenic 50.0%
AACAACTGTAAGACACCGGG+AGG - chr3_3:73205061-73205080 Msa0471000:intron 50.0%
CTGAACACTTTAGCACCTCC+CGG + chr3_3:73205079-73205098 None:intergenic 50.0%
TATCCTGGTCACTGCCAATC+TGG - chr3_3:73205154-73205173 Msa0471000:CDS 50.0%
ATCCTGGTCACTGCCAATCT+GGG - chr3_3:73205155-73205174 Msa0471000:CDS 50.0%
! TCACTGCCAATCTGGGATGA+AGG - chr3_3:73205162-73205181 Msa0471000:CDS 50.0%
! CACAAGCAGGGATAGGTTGG+TGG + chr3_3:73204461-73204480 None:intergenic 55.0%
GCAGCATCTTACACCGTTGG+AGG - chr3_3:73204741-73204760 Msa0471000:CDS 55.0%
TCTTACACCGTTGGAGGACC+TGG - chr3_3:73204747-73204766 Msa0471000:CDS 55.0%
CTTACACCGTTGGAGGACCT+GGG - chr3_3:73204748-73204767 Msa0471000:CDS 55.0%
TTACACCGTTGGAGGACCTG+GGG - chr3_3:73204749-73204768 Msa0471000:CDS 55.0%
CACGGCAACCACATTGTGTG+TGG + chr3_3:73205026-73205045 None:intergenic 55.0%
CACACACAATGTGGTTGCCG+TGG - chr3_3:73205024-73205043 Msa0471000:intron 55.0%
GTGGTTGCCGTGGACAAAAG+TGG - chr3_3:73205034-73205053 Msa0471000:intron 55.0%
ATCCCAGATTGGCAGTGACC+AGG + chr3_3:73205160-73205179 None:intergenic 55.0%
! CACAAGCAGGGATAGGTTGG+TGG + chr3_3:73204461-73204480 None:intergenic 55.0%
GCAGCATCTTACACCGTTGG+AGG - chr3_3:73204741-73204760 Msa0471000:CDS 55.0%
TCTTACACCGTTGGAGGACC+TGG - chr3_3:73204747-73204766 Msa0471000:CDS 55.0%
CTTACACCGTTGGAGGACCT+GGG - chr3_3:73204748-73204767 Msa0471000:CDS 55.0%
TTACACCGTTGGAGGACCTG+GGG - chr3_3:73204749-73204768 Msa0471000:CDS 55.0%
CACGGCAACCACATTGTGTG+TGG + chr3_3:73205026-73205045 None:intergenic 55.0%
CACACACAATGTGGTTGCCG+TGG - chr3_3:73205024-73205043 Msa0471000:intron 55.0%
GTGGTTGCCGTGGACAAAAG+TGG - chr3_3:73205034-73205053 Msa0471000:intron 55.0%
ATCCCAGATTGGCAGTGACC+AGG + chr3_3:73205160-73205179 None:intergenic 55.0%
TACACCGTTGGAGGACCTGG+GGG - chr3_3:73204750-73204769 Msa0471000:CDS 60.0%
TACACCGTTGGAGGACCTGG+GGG - chr3_3:73204750-73204769 Msa0471000:CDS 60.0%
CCATCCCCCAGGTCCTCCAA+CGG + chr3_3:73204757-73204776 None:intergenic 65.0%
CCATCCCCCAGGTCCTCCAA+CGG + chr3_3:73204757-73204776 None:intergenic 65.0%
CCGTTGGAGGACCTGGGGGA+TGG - chr3_3:73204754-73204773 Msa0471000:CDS 70.0%
CCGTTGGAGGACCTGGGGGA+TGG - chr3_3:73204754-73204773 Msa0471000:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 73204340 73205603 73204340 ID=Msa0471000;Name=Msa0471000
chr3_3 mRNA 73204340 73205603 73204340 ID=Msa0471000-mRNA-1;Parent=Msa0471000;Name=Msa0471000-mRNA-1;_AED=0.01;_eAED=0.01;_QI=314|1|1|1|1|1|2|399|126
chr3_3 exon 73204340 73204938 73204340 ID=Msa0471000-mRNA-1:exon:18540;Parent=Msa0471000-mRNA-1
chr3_3 exon 73205109 73205603 73205109 ID=Msa0471000-mRNA-1:exon:18539;Parent=Msa0471000-mRNA-1
chr3_3 five_prime_UTR 73205290 73205603 73205290 ID=Msa0471000-mRNA-1:five_prime_utr;Parent=Msa0471000-mRNA-1
chr3_3 CDS 73205109 73205289 73205109 ID=Msa0471000-mRNA-1:cds;Parent=Msa0471000-mRNA-1
chr3_3 CDS 73204739 73204938 73204739 ID=Msa0471000-mRNA-1:cds;Parent=Msa0471000-mRNA-1
chr3_3 three_prime_UTR 73204340 73204738 73204340 ID=Msa0471000-mRNA-1:three_prime_utr;Parent=Msa0471000-mRNA-1
Gene Sequence

>Msa0471000

ATGACTGAGGGAAGAGGCAGTGCATCTATGAACATGGTCACTGTAATTTCACTGCTGTGCCTTTTGGTTCTGGCTGAAAGTGCTAATGCAGCATCTTACACCGTTGGAGGACCTGGGGGATGGACATACAAAACTGATACTTGGCCTAATGGAAAAAAGTTTAAAGCTGGTGATGTGCTCAGCTTCAACTATGATTCAACCACACACAATGTGGTTGCCGTGGACAAAAGTGGATACAACAACTGTAAGACACCGGGAGGTGCTAAAGTGTTCAGTTCAGGGAATGACCAAATCAGGCTATCAAGAGGACAAAACTACTTCATATGCAGCTATCCTGGTCACTGCCAATCTGGGATGAAGGTTTCCATCTATGCAGTTTAG
Protein sequence

>Msa0471000

MTEGRGSASMNMVTVISLLCLLVLAESANAASYTVGGPGGWTYKTDTWPNGKKFKAGDVLSFNYDSTTHNVVAVDKSGYNNCKTPGGAKVFSSGNDQIRLSRGQNYFICSYPGHCQSGMKVSIYAV*