AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1455640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1455640 MtrunA17_Chr3g0125791 96.825 126 4 0 1 126 1 126 4.39e-89 254
Msa1455640 MtrunA17_Chr3g0125761 66.400 125 42 0 1 125 1 125 4.13e-61 183
Msa1455640 MtrunA17_Chr8g0380901 62.698 126 40 2 1 125 1 120 9.11e-55 167
Msa1455640 MtrunA17_Chr2g0301431 47.619 126 63 2 1 125 1 124 1.21e-39 129
Msa1455640 MtrunA17_Chr8g0380711 55.882 102 44 1 25 125 20 121 3.51e-38 125
Msa1455640 MtrunA17_Chr5g0394321 37.607 117 64 3 14 123 7 121 3.00e-22 86.7
Msa1455640 MtrunA17_Chr8g0335531 40.984 122 66 4 10 126 1 121 1.63e-21 84.7
Msa1455640 MtrunA17_Chr1g0205701 37.168 113 62 3 18 123 10 120 1.27e-20 82.4
Msa1455640 MtrunA17_Chr8g0335571 39.344 122 68 4 10 126 1 121 1.70e-19 79.7
Msa1455640 MtrunA17_Chr1g0177341 35.780 109 66 3 19 123 11 119 5.64e-19 77.4
Msa1455640 MtrunA17_Chr1g0177331 35.780 109 66 3 19 123 11 119 5.64e-19 77.4
Msa1455640 MtrunA17_Chr7g0253681 34.167 120 75 3 8 123 2 121 1.54e-18 76.3
Msa1455640 MtrunA17_Chr7g0253731 32.143 112 72 3 9 117 2 112 1.93e-18 76.3
Msa1455640 MtrunA17_Chr4g0064041 39.394 99 53 2 32 123 190 288 4.72e-18 78.2
Msa1455640 MtrunA17_Chr4g0064041 34.351 131 68 4 12 126 1 129 9.31e-16 72.0
Msa1455640 MtrunA17_Chr7g0253701 34.167 120 74 4 8 123 2 120 1.05e-17 74.3
Msa1455640 MtrunA17_Chr7g0253671 34.167 120 74 4 8 123 2 120 1.05e-17 74.3
Msa1455640 MtrunA17_Chr3g0132441 38.393 112 65 3 15 123 8 118 2.52e-17 73.9
Msa1455640 MtrunA17_Chr2g0306461 35.484 124 69 4 10 123 1 123 3.69e-17 73.6
Msa1455640 MtrunA17_Chr7g0253761 34.454 119 73 3 10 123 1 119 6.01e-17 72.8
Msa1455640 MtrunA17_Chr1g0211101 34.483 116 71 4 12 123 13 127 1.16e-16 72.0
Msa1455640 MtrunA17_Chr2g0323021 35.897 117 68 5 11 123 5 118 1.16e-16 72.8
Msa1455640 MtrunA17_Chr7g0253641 35.185 108 67 2 19 123 12 119 1.47e-16 72.8
Msa1455640 MtrunA17_Chr3g0132701 38.053 113 66 3 15 123 8 120 1.48e-16 72.4
Msa1455640 MtrunA17_Chr6g0459441 40.816 98 53 4 30 123 23 119 3.73e-16 70.5
Msa1455640 MtrunA17_Chr4g0033131 31.405 121 70 3 16 123 7 127 1.84e-15 68.6
Msa1455640 MtrunA17_Chr4g0033531 31.405 121 70 3 16 123 7 127 1.84e-15 68.6
Msa1455640 MtrunA17_Chr3g0132511 37.168 113 67 3 15 123 8 120 1.91e-15 69.7
Msa1455640 MtrunA17_Chr6g0483981 33.333 120 71 2 15 125 9 128 2.83e-15 70.9
Msa1455640 MtrunA17_Chr3g0132431 37.500 112 66 3 15 123 8 118 5.25e-15 67.8
Msa1455640 MtrunA17_Chr7g0253631 34.454 119 73 3 10 123 1 119 1.30e-14 67.8
Msa1455640 MtrunA17_Chr6g0453801 32.520 123 79 2 7 125 9 131 1.39e-14 66.2
Msa1455640 MtrunA17_Chr6g0453921 31.579 133 81 3 3 125 2 134 1.96e-14 66.2
Msa1455640 MtrunA17_Chr6g0481491 30.769 104 68 3 23 123 18 120 4.81e-14 65.5
Msa1455640 MtrunA17_Chr4g0001571 32.653 98 64 1 30 125 26 123 4.16e-13 62.4
Msa1455640 MtrunA17_Chr4g0041811 28.800 125 82 2 9 126 8 132 5.90e-13 62.8
Msa1455640 MtrunA17_Chr8g0387804 27.350 117 79 2 13 123 9 125 4.56e-12 58.9
Msa1455640 MtrunA17_Chr5g0440711 30.328 122 80 3 7 123 3 124 2.05e-11 57.4
Msa1455640 MtrunA17_Chr6g0453791 28.455 123 84 3 5 123 2 124 3.73e-11 57.4
Msa1455640 MtrunA17_Chr4g0064031 33.019 106 59 4 26 120 21 125 7.24e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1455640 AT2G02850.1 54.264 129 56 1 1 126 1 129 1.32e-47 149
Msa1455640 AT1G17800.1 38.095 126 72 3 7 126 12 137 1.45e-26 96.7
Msa1455640 AT2G32300.1 37.705 122 72 3 8 126 2 122 4.58e-23 90.9
Msa1455640 AT2G32300.2 37.705 122 72 3 8 126 32 152 7.10e-23 90.9
Msa1455640 AT5G26330.1 34.426 122 75 2 10 126 1 122 1.60e-22 87.8
Msa1455640 AT3G17675.1 35.246 122 76 2 5 123 4 125 1.41e-21 83.6
Msa1455640 AT5G07475.1 38.739 111 61 4 19 123 13 122 5.15e-20 81.3
Msa1455640 AT3G60280.1 41.237 97 53 3 30 123 21 116 1.40e-19 80.9
Msa1455640 AT3G27200.1 36.364 121 72 4 10 126 3 122 2.51e-19 79.0
Msa1455640 AT2G44790.1 40.741 108 54 5 24 126 23 125 1.47e-17 75.1
Msa1455640 AT3G60270.1 37.113 97 57 3 30 123 23 118 4.35e-17 73.6
Msa1455640 AT2G25060.1 32.773 119 72 3 16 126 14 132 1.98e-15 69.3
Msa1455640 AT2G31050.1 33.043 115 72 3 17 126 12 126 2.21e-15 69.3
Msa1455640 AT2G26720.1 30.579 121 79 3 11 126 6 126 3.91e-15 68.9
Msa1455640 AT4G31840.1 29.134 127 82 3 8 126 2 128 6.87e-15 67.8
Msa1455640 AT1G72230.1 35.345 116 69 4 11 123 3 115 2.08e-13 63.9
Msa1455640 AT3G20570.1 30.952 126 80 3 4 123 2 126 2.90e-13 63.9
Msa1455640 AT5G20230.1 34.000 100 60 2 33 126 25 124 9.35e-13 62.4
Msa1455640 AT2G27035.1 32.500 120 60 6 16 123 12 122 4.43e-12 60.1
Msa1455640 AT4G30590.1 27.907 129 81 4 10 126 1 129 1.39e-11 59.3
Msa1455640 AT1G22480.1 37.069 116 64 5 12 123 1 111 2.24e-11 58.2
Msa1455640 AT5G15350.1 29.060 117 78 3 12 123 7 123 5.75e-11 57.4

Find 69 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTCATGCTGTTATTTATA+TGG 0.243835 tig0036412:+8861 None:intergenic
AAGATCCCTTTGTTTGTTTA+CGG 0.244966 tig0036412:-8667 Msa1455640:three_prime_UTR
TGAAAACTTGAAATTATTTA+CGG 0.262358 tig0036412:+8607 None:intergenic
TTTGGTGATCAATATGTAAA+AGG 0.277801 tig0036412:-8815 Msa1455640:three_prime_UTR
AGACTTATCCCTTTATATAA+TGG 0.283252 tig0036412:+9598 None:intergenic
GTAGTTAATTAGTTAATTAA+TGG 0.284468 tig0036412:-8918 Msa1455640:three_prime_UTR
AATTTCACTGCTGTGCCTTT+TGG 0.295835 tig0036412:-9451 Msa1455640:CDS
CTACTAGCTATATGTTGATT+TGG 0.303864 tig0036412:-8833 Msa1455640:three_prime_UTR
AATTAATAAAACTCCCGTTT+TGG 0.313421 tig0036412:-8572 Msa1455640:three_prime_UTR
TCATCAATTCCATTATATAA+AGG 0.321055 tig0036412:-9607 Msa1455640:five_prime_UTR
TATCCTGGTCACTGCCAATC+TGG 0.329505 tig0036412:-8995 Msa1455640:CDS
GTCCTCTTGATAGCCTGATT+TGG 0.349417 tig0036412:+9038 None:intergenic
CATCAATTCCATTATATAAA+GGG 0.362055 tig0036412:-9606 Msa1455640:five_prime_UTR
AAAACTGATACTTGGCCTAA+TGG 0.367231 tig0036412:-9366 Msa1455640:CDS
GGTGCTAAAGTGTTCAGTTC+AGG 0.375248 tig0036412:-9067 Msa1455640:CDS
CATATTCTCACTATTTCTCT+TGG 0.410921 tig0036412:+9515 None:intergenic
AGAGGACTAAACTGCATAGA+TGG 0.414690 tig0036412:+8961 None:intergenic
AAAATCTGAGGGACCAAAAC+GGG 0.432586 tig0036412:+8559 None:intergenic
TCTTACACCGTTGGAGGACC+TGG 0.442628 tig0036412:-9402 Msa1455640:CDS
GTTCAGGGAATGACCAAATC+AGG 0.451771 tig0036412:-9051 Msa1455640:CDS
TTCTAGTGAGATGGTTGAGT+TGG 0.453959 tig0036412:+9563 None:intergenic
TAAATAACAGCATGAGTACT+AGG 0.471252 tig0036412:-8856 Msa1455640:three_prime_UTR
GATGGTTGAGTTGGTTTGCA+AGG 0.474704 tig0036412:+9572 None:intergenic
TACTTCATATGCAGCTATCC+TGG 0.480047 tig0036412:-9010 Msa1455640:CDS
GAGAATATGACTGAGGGAAG+AGG 0.490305 tig0036412:-9501 Msa1455640:exon
ATCCTGGTCACTGCCAATCT+GGG 0.503016 tig0036412:-8994 Msa1455640:CDS
TGATGAATCTGTTGAGCTAT+GGG 0.503893 tig0036412:+9624 None:intergenic
CACTTTCAGCCAGAACCAAA+AGG 0.511460 tig0036412:+9436 None:intergenic
GTGCTAAAGTGTTCAGTTCA+GGG 0.512300 tig0036412:-9066 Msa1455640:CDS
TTGATGAATCTGTTGAGCTA+TGG 0.522374 tig0036412:+9623 None:intergenic
ATCCCAGATTGGCAGTGACC+AGG 0.530524 tig0036412:+8992 None:intergenic
GAAAATCTGAGGGACCAAAA+CGG 0.530828 tig0036412:+8558 None:intergenic
TAATGGTCAATTAGCTGTAG+TGG 0.537805 tig0036412:-8901 Msa1455640:three_prime_UTR
GGACATACAAAACTGATACT+TGG 0.540585 tig0036412:-9374 Msa1455640:CDS
TGGAAACCTTCATCCCAGAT+TGG 0.543764 tig0036412:+8981 None:intergenic
TCTCACAAGCAGGGATAGGT+TGG 0.544854 tig0036412:+9688 None:intergenic
CACAAGCAGGGATAGGTTGG+TGG 0.545157 tig0036412:+9691 None:intergenic
TCACTGCCAATCTGGGATGA+AGG 0.545313 tig0036412:-8987 Msa1455640:CDS
CATCTCACTAGAATTGCTCT+AGG 0.545816 tig0036412:-9553 Msa1455640:five_prime_UTR
TGGATCTCACAAGCAGGGAT+AGG 0.547940 tig0036412:+9684 None:intergenic
CTTAGACCGTAAACAAACAA+AGG 0.558115 tig0036412:+8661 None:intergenic
TTAGACCGTAAACAAACAAA+GGG 0.559350 tig0036412:+8662 None:intergenic
CTAGAGCAATTCTAGTGAGA+TGG 0.561240 tig0036412:+9554 None:intergenic
TTGGATGCTACTCACTAGCT+AGG 0.563622 tig0036412:+9779 None:intergenic
GCAGCATCTTACACCGTTGG+AGG 0.574303 tig0036412:-9408 Msa1455640:CDS
GTGGTTGCCGTGGACAAAAG+TGG 0.576000 tig0036412:-9115 Msa1455640:CDS
CTGAACACTTTAGCACCTCC+CGG 0.578857 tig0036412:+9073 None:intergenic
AATGCAGCATCTTACACCGT+TGG 0.578987 tig0036412:-9411 Msa1455640:CDS
CACACACAATGTGGTTGCCG+TGG 0.585202 tig0036412:-9125 Msa1455640:CDS
AATAGTGAGAATATGACTGA+GGG 0.586297 tig0036412:-9507 Msa1455640:exon
CTTACACCGTTGGAGGACCT+GGG 0.588429 tig0036412:-9401 Msa1455640:CDS
AAATAGTGAGAATATGACTG+AGG 0.592419 tig0036412:-9508 Msa1455640:exon
ATCAATATGTAAAAGGAGTG+TGG 0.597954 tig0036412:-8808 Msa1455640:three_prime_UTR
ATATTGTGGATCTCACAAGC+AGG 0.598727 tig0036412:+9678 None:intergenic
AAGGGAGAGGATGGTAATGT+CGG 0.599256 tig0036412:-9804 Msa1455640:five_prime_UTR
TAGTCAAGTCACATAGCTAG+AGG 0.624989 tig0036412:+8943 None:intergenic
TACAACAACTGTAAGACACC+GGG 0.625426 tig0036412:-9091 Msa1455640:CDS
TTACACCGTTGGAGGACCTG+GGG 0.632787 tig0036412:-9400 Msa1455640:CDS
CACGGCAACCACATTGTGTG+TGG 0.635175 tig0036412:+9126 None:intergenic
TATTGTGGATCTCACAAGCA+GGG 0.635490 tig0036412:+9679 None:intergenic
GACCAAATCAGGCTATCAAG+AGG 0.635565 tig0036412:-9040 Msa1455640:CDS
AGGCAGTGCATCTATGAACA+TGG 0.647845 tig0036412:-9481 Msa1455640:CDS
ATTGTGTAGATTGAATATTG+TGG 0.652337 tig0036412:+9664 None:intergenic
TGATTCAACCACACACAATG+TGG 0.661122 tig0036412:-9134 Msa1455640:CDS
ATACAACAACTGTAAGACAC+CGG 0.661265 tig0036412:-9092 Msa1455640:CDS
TACACCGTTGGAGGACCTGG+GGG 0.694377 tig0036412:-9399 Msa1455640:CDS
TATGTAAAAGGAGTGTGGTG+AGG 0.701233 tig0036412:-8803 Msa1455640:three_prime_UTR
AACAACTGTAAGACACCGGG+AGG 0.711932 tig0036412:-9088 Msa1455640:CDS
GATGAATCTGTTGAGCTATG+GGG 0.737724 tig0036412:+9625 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTAATTACAGTACAAT+TGG - tig0036412:9104-9123 Msa1455640:CDS 15.0%
!! AATTTAATTACAGTACAATT+GGG - tig0036412:9105-9124 Msa1455640:CDS 15.0%
!!! AAAATTGAGAAATATAGTTT+TGG + tig0036412:9144-9163 None:intergenic 15.0%
!!! TCAATTTTACAAAATTGTTT+TGG - tig0036412:9156-9175 Msa1455640:intron 15.0%
!! GTAGTTAATTAGTTAATTAA+TGG - tig0036412:9460-9479 Msa1455640:CDS 15.0%
!!! TTTTGGTTTATTTAATTCAT+TGG - tig0036412:9746-9765 Msa1455640:five_prime_UTR 15.0%
!! TGAAAACTTGAAATTATTTA+CGG + tig0036412:9774-9793 None:intergenic 15.0%
!! TAATTTAATTACAGTACAAT+TGG - tig0036412:9104-9123 Msa1455640:CDS 15.0%
!! AATTTAATTACAGTACAATT+GGG - tig0036412:9105-9124 Msa1455640:CDS 15.0%
!!! AAAATTGAGAAATATAGTTT+TGG + tig0036412:9144-9163 None:intergenic 15.0%
!!! TCAATTTTACAAAATTGTTT+TGG - tig0036412:9156-9175 Msa1455640:intron 15.0%
!! GTAGTTAATTAGTTAATTAA+TGG - tig0036412:9460-9479 Msa1455640:CDS 15.0%
!!! TTTTGGTTTATTTAATTCAT+TGG - tig0036412:9746-9765 Msa1455640:five_prime_UTR 15.0%
!! TGAAAACTTGAAATTATTTA+CGG + tig0036412:9774-9793 None:intergenic 15.0%
!! TCATCAATTCCATTATATAA+AGG - tig0036412:8771-8790 Msa1455640:three_prime_UTR 20.0%
!! CATCAATTCCATTATATAAA+GGG - tig0036412:8772-8791 Msa1455640:three_prime_UTR 20.0%
!! ACTTTAATTATATGTGCATA+TGG - tig0036412:9195-9214 Msa1455640:intron 20.0%
!! TCATCAATTCCATTATATAA+AGG - tig0036412:8771-8790 Msa1455640:three_prime_UTR 20.0%
!! CATCAATTCCATTATATAAA+GGG - tig0036412:8772-8791 Msa1455640:three_prime_UTR 20.0%
!! ACTTTAATTATATGTGCATA+TGG - tig0036412:9195-9214 Msa1455640:intron 20.0%
! ATTGTGTAGATTGAATATTG+TGG + tig0036412:8717-8736 None:intergenic 25.0%
! AGACTTATCCCTTTATATAA+TGG + tig0036412:8783-8802 None:intergenic 25.0%
!!! GCTTTAAACTTTTTTCCATT+AGG + tig0036412:9030-9049 None:intergenic 25.0%
!! AATGGAAAAAAGTTTAAAGC+TGG - tig0036412:9030-9049 Msa1455640:CDS 25.0%
!! TACTCATGCTGTTATTTATA+TGG + tig0036412:9520-9539 None:intergenic 25.0%
!! TTTGGTGATCAATATGTAAA+AGG - tig0036412:9563-9582 Msa1455640:five_prime_UTR 25.0%
!!! GTTTGATATGTTTGTTTTTG+TGG - tig0036412:9621-9640 Msa1455640:five_prime_UTR 25.0%
!!! TACGGTCTAAGTTTTTATTT+TGG - tig0036412:9729-9748 Msa1455640:five_prime_UTR 25.0%
!! AATTAATAAAACTCCCGTTT+TGG - tig0036412:9806-9825 Msa1455640:five_prime_UTR 25.0%
! ATTGTGTAGATTGAATATTG+TGG + tig0036412:8717-8736 None:intergenic 25.0%
! AGACTTATCCCTTTATATAA+TGG + tig0036412:8783-8802 None:intergenic 25.0%
!!! GCTTTAAACTTTTTTCCATT+AGG + tig0036412:9030-9049 None:intergenic 25.0%
!! AATGGAAAAAAGTTTAAAGC+TGG - tig0036412:9030-9049 Msa1455640:CDS 25.0%
!! TACTCATGCTGTTATTTATA+TGG + tig0036412:9520-9539 None:intergenic 25.0%
!! TTTGGTGATCAATATGTAAA+AGG - tig0036412:9563-9582 Msa1455640:five_prime_UTR 25.0%
!!! GTTTGATATGTTTGTTTTTG+TGG - tig0036412:9621-9640 Msa1455640:five_prime_UTR 25.0%
!!! TACGGTCTAAGTTTTTATTT+TGG - tig0036412:9729-9748 Msa1455640:five_prime_UTR 25.0%
!! AATTAATAAAACTCCCGTTT+TGG - tig0036412:9806-9825 Msa1455640:five_prime_UTR 25.0%
! TGGCTTTTCTTCAATAGTTA+AGG + tig0036412:8670-8689 None:intergenic 30.0%
! CATATTCTCACTATTTCTCT+TGG + tig0036412:8866-8885 None:intergenic 30.0%
AAATAGTGAGAATATGACTG+AGG - tig0036412:8870-8889 Msa1455640:three_prime_UTR 30.0%
AATAGTGAGAATATGACTGA+GGG - tig0036412:8871-8890 Msa1455640:three_prime_UTR 30.0%
ATATGGTAATTGATTGTTGC+AGG - tig0036412:9212-9231 Msa1455640:intron 30.0%
TAAATAACAGCATGAGTACT+AGG - tig0036412:9522-9541 Msa1455640:five_prime_UTR 30.0%
! CTACTAGCTATATGTTGATT+TGG - tig0036412:9545-9564 Msa1455640:five_prime_UTR 30.0%
ATCAATATGTAAAAGGAGTG+TGG - tig0036412:9570-9589 Msa1455640:five_prime_UTR 30.0%
! AAGATCCCTTTGTTTGTTTA+CGG - tig0036412:9711-9730 Msa1455640:five_prime_UTR 30.0%
TTAGACCGTAAACAAACAAA+GGG + tig0036412:9719-9738 None:intergenic 30.0%
!!! ACGGGAGTTTTATTAATTGT+GGG + tig0036412:9804-9823 None:intergenic 30.0%
!!! AACGGGAGTTTTATTAATTG+TGG + tig0036412:9805-9824 None:intergenic 30.0%
! TGGCTTTTCTTCAATAGTTA+AGG + tig0036412:8670-8689 None:intergenic 30.0%
! CATATTCTCACTATTTCTCT+TGG + tig0036412:8866-8885 None:intergenic 30.0%
AAATAGTGAGAATATGACTG+AGG - tig0036412:8870-8889 Msa1455640:three_prime_UTR 30.0%
AATAGTGAGAATATGACTGA+GGG - tig0036412:8871-8890 Msa1455640:three_prime_UTR 30.0%
ATATGGTAATTGATTGTTGC+AGG - tig0036412:9212-9231 Msa1455640:intron 30.0%
TAAATAACAGCATGAGTACT+AGG - tig0036412:9522-9541 Msa1455640:five_prime_UTR 30.0%
! CTACTAGCTATATGTTGATT+TGG - tig0036412:9545-9564 Msa1455640:five_prime_UTR 30.0%
ATCAATATGTAAAAGGAGTG+TGG - tig0036412:9570-9589 Msa1455640:five_prime_UTR 30.0%
! AAGATCCCTTTGTTTGTTTA+CGG - tig0036412:9711-9730 Msa1455640:five_prime_UTR 30.0%
TTAGACCGTAAACAAACAAA+GGG + tig0036412:9719-9738 None:intergenic 30.0%
!!! ACGGGAGTTTTATTAATTGT+GGG + tig0036412:9804-9823 None:intergenic 30.0%
!!! AACGGGAGTTTTATTAATTG+TGG + tig0036412:9805-9824 None:intergenic 30.0%
TGATGAATCTGTTGAGCTAT+GGG + tig0036412:8757-8776 None:intergenic 35.0%
TTGATGAATCTGTTGAGCTA+TGG + tig0036412:8758-8777 None:intergenic 35.0%
GGACATACAAAACTGATACT+TGG - tig0036412:9004-9023 Msa1455640:CDS 35.0%
AAAACTGATACTTGGCCTAA+TGG - tig0036412:9012-9031 Msa1455640:CDS 35.0%
ATACAACAACTGTAAGACAC+CGG - tig0036412:9286-9305 Msa1455640:intron 35.0%
TAATGGTCAATTAGCTGTAG+TGG - tig0036412:9477-9496 Msa1455640:CDS 35.0%
CTTAGACCGTAAACAAACAA+AGG + tig0036412:9720-9739 None:intergenic 35.0%
TGATGAATCTGTTGAGCTAT+GGG + tig0036412:8757-8776 None:intergenic 35.0%
TTGATGAATCTGTTGAGCTA+TGG + tig0036412:8758-8777 None:intergenic 35.0%
GGACATACAAAACTGATACT+TGG - tig0036412:9004-9023 Msa1455640:CDS 35.0%
AAAACTGATACTTGGCCTAA+TGG - tig0036412:9012-9031 Msa1455640:CDS 35.0%
ATACAACAACTGTAAGACAC+CGG - tig0036412:9286-9305 Msa1455640:intron 35.0%
TAATGGTCAATTAGCTGTAG+TGG - tig0036412:9477-9496 Msa1455640:CDS 35.0%
CTTAGACCGTAAACAAACAA+AGG + tig0036412:9720-9739 None:intergenic 35.0%
TATTGTGGATCTCACAAGCA+GGG + tig0036412:8702-8721 None:intergenic 40.0%
ATATTGTGGATCTCACAAGC+AGG + tig0036412:8703-8722 None:intergenic 40.0%
GATGAATCTGTTGAGCTATG+GGG + tig0036412:8756-8775 None:intergenic 40.0%
TTCTAGTGAGATGGTTGAGT+TGG + tig0036412:8818-8837 None:intergenic 40.0%
CTAGAGCAATTCTAGTGAGA+TGG + tig0036412:8827-8846 None:intergenic 40.0%
CATCTCACTAGAATTGCTCT+AGG - tig0036412:8825-8844 Msa1455640:three_prime_UTR 40.0%
! AATTTCACTGCTGTGCCTTT+TGG - tig0036412:8927-8946 Msa1455640:three_prime_UTR 40.0%
TGATTCAACCACACACAATG+TGG - tig0036412:9244-9263 Msa1455640:intron 40.0%
!! GTTGTATCCACTTTTGTCCA+CGG + tig0036412:9273-9292 None:intergenic 40.0%
TACAACAACTGTAAGACACC+GGG - tig0036412:9287-9306 Msa1455640:intron 40.0%
! GTGCTAAAGTGTTCAGTTCA+GGG - tig0036412:9312-9331 Msa1455640:intron 40.0%
TACTTCATATGCAGCTATCC+TGG - tig0036412:9368-9387 Msa1455640:CDS 40.0%
AGAGGACTAAACTGCATAGA+TGG + tig0036412:9420-9439 None:intergenic 40.0%
TAGTCAAGTCACATAGCTAG+AGG + tig0036412:9438-9457 None:intergenic 40.0%
! TATGTAAAAGGAGTGTGGTG+AGG - tig0036412:9575-9594 Msa1455640:five_prime_UTR 40.0%
!!! CCATCCTCTCCCTTTTTTTT+GGG + tig0036412:8568-8587 None:intergenic 40.0%
!!! ACCATCCTCTCCCTTTTTTT+TGG + tig0036412:8569-8588 None:intergenic 40.0%
TATTGTGGATCTCACAAGCA+GGG + tig0036412:8702-8721 None:intergenic 40.0%
ATATTGTGGATCTCACAAGC+AGG + tig0036412:8703-8722 None:intergenic 40.0%
GATGAATCTGTTGAGCTATG+GGG + tig0036412:8756-8775 None:intergenic 40.0%
TTCTAGTGAGATGGTTGAGT+TGG + tig0036412:8818-8837 None:intergenic 40.0%
CTAGAGCAATTCTAGTGAGA+TGG + tig0036412:8827-8846 None:intergenic 40.0%
CATCTCACTAGAATTGCTCT+AGG - tig0036412:8825-8844 Msa1455640:three_prime_UTR 40.0%
! AATTTCACTGCTGTGCCTTT+TGG - tig0036412:8927-8946 Msa1455640:three_prime_UTR 40.0%
TGATTCAACCACACACAATG+TGG - tig0036412:9244-9263 Msa1455640:intron 40.0%
!! GTTGTATCCACTTTTGTCCA+CGG + tig0036412:9273-9292 None:intergenic 40.0%
TACAACAACTGTAAGACACC+GGG - tig0036412:9287-9306 Msa1455640:intron 40.0%
! GTGCTAAAGTGTTCAGTTCA+GGG - tig0036412:9312-9331 Msa1455640:intron 40.0%
TACTTCATATGCAGCTATCC+TGG - tig0036412:9368-9387 Msa1455640:CDS 40.0%
AGAGGACTAAACTGCATAGA+TGG + tig0036412:9420-9439 None:intergenic 40.0%
TAGTCAAGTCACATAGCTAG+AGG + tig0036412:9438-9457 None:intergenic 40.0%
! TATGTAAAAGGAGTGTGGTG+AGG - tig0036412:9575-9594 Msa1455640:five_prime_UTR 40.0%
! AAGGGAGAGGATGGTAATGT+CGG - tig0036412:8574-8593 Msa1455640:three_prime_UTR 45.0%
TTGGATGCTACTCACTAGCT+AGG + tig0036412:8602-8621 None:intergenic 45.0%
!! GATGGTTGAGTTGGTTTGCA+AGG + tig0036412:8809-8828 None:intergenic 45.0%
GAGAATATGACTGAGGGAAG+AGG - tig0036412:8877-8896 Msa1455640:three_prime_UTR 45.0%
AGGCAGTGCATCTATGAACA+TGG - tig0036412:8897-8916 Msa1455640:three_prime_UTR 45.0%
CACTTTCAGCCAGAACCAAA+AGG + tig0036412:8945-8964 None:intergenic 45.0%
AATGCAGCATCTTACACCGT+TGG - tig0036412:8967-8986 Msa1455640:CDS 45.0%
! GGTGCTAAAGTGTTCAGTTC+AGG - tig0036412:9311-9330 Msa1455640:intron 45.0%
GTTCAGGGAATGACCAAATC+AGG - tig0036412:9327-9346 Msa1455640:intron 45.0%
GACCAAATCAGGCTATCAAG+AGG - tig0036412:9338-9357 Msa1455640:CDS 45.0%
! GTCCTCTTGATAGCCTGATT+TGG + tig0036412:9343-9362 None:intergenic 45.0%
TGGAAACCTTCATCCCAGAT+TGG + tig0036412:9400-9419 None:intergenic 45.0%
! TTTTTGTGGTGTGCTGTGTG+AGG - tig0036412:9635-9654 Msa1455640:five_prime_UTR 45.0%
! AAGGGAGAGGATGGTAATGT+CGG - tig0036412:8574-8593 Msa1455640:three_prime_UTR 45.0%
TTGGATGCTACTCACTAGCT+AGG + tig0036412:8602-8621 None:intergenic 45.0%
!! GATGGTTGAGTTGGTTTGCA+AGG + tig0036412:8809-8828 None:intergenic 45.0%
GAGAATATGACTGAGGGAAG+AGG - tig0036412:8877-8896 Msa1455640:three_prime_UTR 45.0%
AGGCAGTGCATCTATGAACA+TGG - tig0036412:8897-8916 Msa1455640:three_prime_UTR 45.0%
CACTTTCAGCCAGAACCAAA+AGG + tig0036412:8945-8964 None:intergenic 45.0%
AATGCAGCATCTTACACCGT+TGG - tig0036412:8967-8986 Msa1455640:CDS 45.0%
! GGTGCTAAAGTGTTCAGTTC+AGG - tig0036412:9311-9330 Msa1455640:intron 45.0%
GTTCAGGGAATGACCAAATC+AGG - tig0036412:9327-9346 Msa1455640:intron 45.0%
GACCAAATCAGGCTATCAAG+AGG - tig0036412:9338-9357 Msa1455640:CDS 45.0%
! GTCCTCTTGATAGCCTGATT+TGG + tig0036412:9343-9362 None:intergenic 45.0%
TGGAAACCTTCATCCCAGAT+TGG + tig0036412:9400-9419 None:intergenic 45.0%
! TTTTTGTGGTGTGCTGTGTG+AGG - tig0036412:9635-9654 Msa1455640:five_prime_UTR 45.0%
!!! TTTTTTTTTTTTTTTAAAGT+TGG + tig0036412:8621-8640 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTAAAGT+TGG + tig0036412:8621-8640 None:intergenic 5.0%
TCTCACAAGCAGGGATAGGT+TGG + tig0036412:8693-8712 None:intergenic 50.0%
TGGATCTCACAAGCAGGGAT+AGG + tig0036412:8697-8716 None:intergenic 50.0%
!!! ACTGCTGTGCCTTTTGGTTC+TGG - tig0036412:8933-8952 Msa1455640:three_prime_UTR 50.0%
! GTTTTGTATGTCCATCCCCC+AGG + tig0036412:8997-9016 None:intergenic 50.0%
AACAACTGTAAGACACCGGG+AGG - tig0036412:9290-9309 Msa1455640:intron 50.0%
CTGAACACTTTAGCACCTCC+CGG + tig0036412:9308-9327 None:intergenic 50.0%
TATCCTGGTCACTGCCAATC+TGG - tig0036412:9383-9402 Msa1455640:CDS 50.0%
ATCCTGGTCACTGCCAATCT+GGG - tig0036412:9384-9403 Msa1455640:CDS 50.0%
! TCACTGCCAATCTGGGATGA+AGG - tig0036412:9391-9410 Msa1455640:CDS 50.0%
TCTCACAAGCAGGGATAGGT+TGG + tig0036412:8693-8712 None:intergenic 50.0%
TGGATCTCACAAGCAGGGAT+AGG + tig0036412:8697-8716 None:intergenic 50.0%
!!! ACTGCTGTGCCTTTTGGTTC+TGG - tig0036412:8933-8952 Msa1455640:three_prime_UTR 50.0%
! GTTTTGTATGTCCATCCCCC+AGG + tig0036412:8997-9016 None:intergenic 50.0%
AACAACTGTAAGACACCGGG+AGG - tig0036412:9290-9309 Msa1455640:intron 50.0%
CTGAACACTTTAGCACCTCC+CGG + tig0036412:9308-9327 None:intergenic 50.0%
TATCCTGGTCACTGCCAATC+TGG - tig0036412:9383-9402 Msa1455640:CDS 50.0%
ATCCTGGTCACTGCCAATCT+GGG - tig0036412:9384-9403 Msa1455640:CDS 50.0%
! TCACTGCCAATCTGGGATGA+AGG - tig0036412:9391-9410 Msa1455640:CDS 50.0%
! CACAAGCAGGGATAGGTTGG+TGG + tig0036412:8690-8709 None:intergenic 55.0%
GCAGCATCTTACACCGTTGG+AGG - tig0036412:8970-8989 Msa1455640:CDS 55.0%
TCTTACACCGTTGGAGGACC+TGG - tig0036412:8976-8995 Msa1455640:CDS 55.0%
CTTACACCGTTGGAGGACCT+GGG - tig0036412:8977-8996 Msa1455640:CDS 55.0%
TTACACCGTTGGAGGACCTG+GGG - tig0036412:8978-8997 Msa1455640:CDS 55.0%
CACGGCAACCACATTGTGTG+TGG + tig0036412:9255-9274 None:intergenic 55.0%
CACACACAATGTGGTTGCCG+TGG - tig0036412:9253-9272 Msa1455640:intron 55.0%
GTGGTTGCCGTGGACAAAAG+TGG - tig0036412:9263-9282 Msa1455640:intron 55.0%
ATCCCAGATTGGCAGTGACC+AGG + tig0036412:9389-9408 None:intergenic 55.0%
! CACAAGCAGGGATAGGTTGG+TGG + tig0036412:8690-8709 None:intergenic 55.0%
GCAGCATCTTACACCGTTGG+AGG - tig0036412:8970-8989 Msa1455640:CDS 55.0%
TCTTACACCGTTGGAGGACC+TGG - tig0036412:8976-8995 Msa1455640:CDS 55.0%
CTTACACCGTTGGAGGACCT+GGG - tig0036412:8977-8996 Msa1455640:CDS 55.0%
TTACACCGTTGGAGGACCTG+GGG - tig0036412:8978-8997 Msa1455640:CDS 55.0%
CACGGCAACCACATTGTGTG+TGG + tig0036412:9255-9274 None:intergenic 55.0%
CACACACAATGTGGTTGCCG+TGG - tig0036412:9253-9272 Msa1455640:intron 55.0%
GTGGTTGCCGTGGACAAAAG+TGG - tig0036412:9263-9282 Msa1455640:intron 55.0%
ATCCCAGATTGGCAGTGACC+AGG + tig0036412:9389-9408 None:intergenic 55.0%
TACACCGTTGGAGGACCTGG+GGG - tig0036412:8979-8998 Msa1455640:CDS 60.0%
TACACCGTTGGAGGACCTGG+GGG - tig0036412:8979-8998 Msa1455640:CDS 60.0%
CCATCCCCCAGGTCCTCCAA+CGG + tig0036412:8986-9005 None:intergenic 65.0%
CCATCCCCCAGGTCCTCCAA+CGG + tig0036412:8986-9005 None:intergenic 65.0%
CCGTTGGAGGACCTGGGGGA+TGG - tig0036412:8983-9002 Msa1455640:CDS 70.0%
CCGTTGGAGGACCTGGGGGA+TGG - tig0036412:8983-9002 Msa1455640:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0036412 gene 8568 9832 8568 ID=Msa1455640;Name=Msa1455640
tig0036412 mRNA 8568 9832 8568 ID=Msa1455640-mRNA-1;Parent=Msa1455640;Name=Msa1455640-mRNA-1;_AED=0.01;_eAED=0.01;_QI=315|1|1|1|1|1|2|399|126
tig0036412 exon 8568 9166 8568 ID=Msa1455640-mRNA-1:exon:20349;Parent=Msa1455640-mRNA-1
tig0036412 exon 9337 9832 9337 ID=Msa1455640-mRNA-1:exon:20348;Parent=Msa1455640-mRNA-1
tig0036412 five_prime_UTR 9518 9832 9518 ID=Msa1455640-mRNA-1:five_prime_utr;Parent=Msa1455640-mRNA-1
tig0036412 CDS 9337 9517 9337 ID=Msa1455640-mRNA-1:cds;Parent=Msa1455640-mRNA-1
tig0036412 CDS 8967 9166 8967 ID=Msa1455640-mRNA-1:cds;Parent=Msa1455640-mRNA-1
tig0036412 three_prime_UTR 8568 8966 8568 ID=Msa1455640-mRNA-1:three_prime_utr;Parent=Msa1455640-mRNA-1
Gene Sequence

>Msa1455640

ATGACTGAGGGAAGAGGCAGTGCATCTATGAACATGGTCACTGTAATTTCACTGCTGTGCCTTTTGGTTCTGGCTGAAAGTGCTAATGCAGCATCTTACACCGTTGGAGGACCTGGGGGATGGACATACAAAACTGATACTTGGCCTAATGGAAAAAAGTTTAAAGCTGGTGATGTGCTCAGCTTCAACTATGATTCAACCACACACAATGTGGTTGCCGTGGACAAAAGTGGATACAACAACTGTAAGACACCGGGAGGTGCTAAAGTGTTCAGTTCAGGGAATGACCAAATCAGGCTATCAAGAGGACAAAACTACTTCATATGCAGCTATCCTGGTCACTGCCAATCTGGGATGAAGGTTTCCATCTATGCAGTTTAG
Protein sequence

>Msa1455640

MTEGRGSASMNMVTVISLLCLLVLAESANAASYTVGGPGGWTYKTDTWPNGKKFKAGDVLSFNYDSTTHNVVAVDKSGYNNCKTPGGAKVFSSGNDQIRLSRGQNYFICSYPGHCQSGMKVSIYAV*