AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0996830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0996830 MtrunA17_Chr6g0481761 99.690 323 1 0 1 323 1 323 0.0 653
Msa0996830 MtrunA17_Chr2g0294381 32.710 214 132 3 116 318 104 316 2.38e-22 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0996830 AT5G23250.1 85.670 321 41 2 5 323 24 341 0.0 557
Msa0996830 AT5G08300.1 87.296 307 37 1 19 323 40 346 0.0 553
Msa0996830 AT5G23250.2 87.273 275 30 2 5 277 24 295 2.27e-174 485
Msa0996830 AT5G23250.3 85.590 229 28 2 5 231 24 249 1.03e-140 398
Msa0996830 AT3G06650.1 31.776 214 134 3 116 318 104 316 1.81e-21 95.5
Msa0996830 AT3G06650.2 31.776 214 134 3 116 318 104 316 1.81e-21 95.5
Msa0996830 AT5G49460.2 32.243 214 133 3 116 318 104 316 3.00e-21 94.7
Msa0996830 AT5G49460.1 32.243 214 133 3 116 318 104 316 3.00e-21 94.7

Find 114 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACCCTGTGCCTCCTTTC+TGG 0.145644 6_4:+75550181 None:intergenic
ATTGGAATGTATCGAATTTC+CGG 0.233070 6_4:+75545833 None:intergenic
ACCGAACAAGCGATCGAATA+TGG 0.237150 6_4:-75549926 Msa0996830:CDS
AATTCAGGTATTATTCTTAT+TGG 0.247983 6_4:-75547356 Msa0996830:intron
ATTATTCTTATTGGAGAGAT+AGG 0.288969 6_4:-75547347 Msa0996830:CDS
CAGAAGTGAAAGTGTGATTT+GGG 0.301303 6_4:+75550127 None:intergenic
GATACATGTAGTATGATAAT+TGG 0.302468 6_4:-75545655 Msa0996830:three_prime_UTR
AAAGGCGGAACGGAACATTT+AGG 0.302614 6_4:-75549872 Msa0996830:CDS
CCTGTTGTTGCATTTATTGC+TGG 0.315893 6_4:-75546702 Msa0996830:CDS
GATCGAATATGGAACGAATA+TGG 0.316829 6_4:-75549915 Msa0996830:CDS
TGGTCCTATCAAACGAGTTT+TGG 0.319821 6_4:+75548278 None:intergenic
AAGCCCGGGGAATGTAAAAT+TGG 0.326008 6_4:-75548237 Msa0996830:CDS
TGATTCCCGTTGATAGTAAT+GGG 0.328101 6_4:-75545930 Msa0996830:three_prime_UTR
AAGGCGGAACGGAACATTTA+GGG 0.329459 6_4:-75549871 Msa0996830:CDS
GTGGAGTCCCCAGCTAAAAT+TGG 0.338774 6_4:-75546066 Msa0996830:CDS
AACGGAACATTTAGGGCTTC+CGG 0.344291 6_4:-75549864 Msa0996830:CDS
TCAGAAGTGAAAGTGTGATT+TGG 0.350427 6_4:+75550126 None:intergenic
TCCATATTCGATCGCTTGTT+CGG 0.380007 6_4:+75549925 None:intergenic
ATTGGAATTGTTTCTCGTTC+TGG 0.384214 6_4:-75548180 Msa0996830:CDS
AAAGATGTACCTTCTGTTTG+AGG 0.393263 6_4:+75547534 None:intergenic
TGAGAAGTTGCGGCGGTTGT+TGG 0.395891 6_4:+75550033 None:intergenic
CAAACAACTGCTGTTGGCCT+TGG 0.397173 6_4:-75547639 Msa0996830:CDS
TGAGCTCCAGCTTGTGGCGC+TGG 0.399183 6_4:-75545810 Msa0996830:three_prime_UTR
TGTTCCGTTCCGCCTTTCTT+CGG 0.405095 6_4:+75549878 None:intergenic
AACAAAGTTTGTTCCATTGA+AGG 0.409176 6_4:+75547584 None:intergenic
AGGGATTCCTCAACATGATA+TGG 0.409706 6_4:-75549705 Msa0996830:intron
GGACGCTGCTGCTCTAATTA+AGG 0.414347 6_4:-75547309 Msa0996830:intron
CAATCCAAAACTCGTTTGAT+AGG 0.420569 6_4:-75548282 Msa0996830:CDS
AAGACAAAATCAGCACCCTT+AGG 0.428915 6_4:-75546107 Msa0996830:CDS
CAAGGCCAACAGCAGTTGTT+TGG 0.431303 6_4:+75547640 None:intergenic
ATCAGCACCCTTAGGGAAGC+TGG 0.437867 6_4:-75546099 Msa0996830:CDS
GCCACAAGCTGGAGCTCAAT+TGG 0.438732 6_4:+75545815 None:intergenic
TTGATGATACCAGGGCAGTT+TGG 0.446440 6_4:+75548258 None:intergenic
TCCACATGTGTGGGCATTGG+AGG 0.447053 6_4:-75547612 Msa0996830:CDS
TTGATTCCCGTTGATAGTAA+TGG 0.447910 6_4:-75545931 Msa0996830:three_prime_UTR
CCGGGTTACATTCACAAACC+TGG 0.447993 6_4:-75548207 Msa0996830:CDS
AAAATCCCATTACTATCAAC+GGG 0.451333 6_4:+75545925 None:intergenic
AGCTATTGTGTCGGGTGGCA+AGG 0.452187 6_4:-75546139 Msa0996830:CDS
ACAAAGTTTGTTCCATTGAA+GGG 0.461996 6_4:+75547585 None:intergenic
AGGCTTAGTGCAATAGTTTG+AGG 0.468890 6_4:-75546013 Msa0996830:exon
TCAGTCCAGAAAGGAGGCAC+AGG 0.472249 6_4:-75550186 Msa0996830:five_prime_UTR
GTGATCTGTCAAGGAATAAC+CGG 0.473606 6_4:-75549968 Msa0996830:CDS
TGCCCTGGTATCATCAAGCC+CGG 0.475481 6_4:-75548252 Msa0996830:CDS
ATTCACAAACCTGGGCGCAT+TGG 0.479565 6_4:-75548198 Msa0996830:CDS
CCAGCAATAAATGCAACAAC+AGG 0.479962 6_4:+75546702 None:intergenic
TTCTTGTGCAGCTATTGTGT+CGG 0.483534 6_4:-75546148 Msa0996830:intron
CTTGTTGTTTGTATTACTGA+AGG 0.486594 6_4:-75549725 Msa0996830:CDS
GGAAGCGGTTGAAGCGGAGT+TGG 0.489889 6_4:-75549750 Msa0996830:CDS
TACAAACCAGCGCCACAAGC+TGG 0.490129 6_4:+75545804 None:intergenic
CTGTGACTCCAGCTTCCCTA+AGG 0.494628 6_4:+75546091 None:intergenic
TGCTGCTGCTATTATGGAAG+CGG 0.495050 6_4:-75549765 Msa0996830:CDS
TCTTGTGCAGCTATTGTGTC+GGG 0.495233 6_4:-75546147 Msa0996830:intron
TCCCCGGGCTTGATGATACC+AGG 0.502694 6_4:+75548249 None:intergenic
GAAGTTGCGGCGGTTGTTGG+AGG 0.505830 6_4:+75550036 None:intergenic
CAATCCACATGTGTGGGCAT+TGG 0.513242 6_4:-75547615 Msa0996830:CDS
TGTAAAATTGGAATCATGCC+GGG 0.516403 6_4:-75548225 Msa0996830:CDS
TCCGCAACAGAATTGAAAAC+CGG 0.517721 6_4:+75549845 None:intergenic
GATCTGCTAGGAACTTTGTA+AGG 0.517985 6_4:+75547556 None:intergenic
AGACAAAATCAGCACCCTTA+GGG 0.519396 6_4:-75546106 Msa0996830:CDS
TCCTCCAATGCCCACACATG+TGG 0.524542 6_4:+75547611 None:intergenic
ATAATAGCAGCAGCAGCAAA+AGG 0.524930 6_4:+75549773 None:intergenic
TCCAATTGAGCTCCAGCTTG+TGG 0.528907 6_4:-75545816 Msa0996830:three_prime_UTR
GCTATTGTGTCGGGTGGCAA+GGG 0.534414 6_4:-75546138 Msa0996830:CDS
GCAGCAGCAGCAAAAGGAGG+AGG 0.535843 6_4:+75549779 None:intergenic
GGTGGTGTTACTCCGAAGAA+AGG 0.539534 6_4:-75549890 Msa0996830:CDS
ATGTAAAATTGGAATCATGC+CGG 0.544331 6_4:-75548226 Msa0996830:CDS
TCGACGAACACCGCCGGAGA+GGG 0.545538 6_4:+75550004 None:intergenic
CTGCTTGAAAATATCAAACA+TGG 0.547001 6_4:+75546038 None:intergenic
ATTGGAGGAGACCCCTTCAA+TGG 0.551942 6_4:-75547597 Msa0996830:CDS
TTGTTGTTTGTATTACTGAA+GGG 0.552535 6_4:-75549724 Msa0996830:CDS
CAAAGTTTGTTCCATTGAAG+GGG 0.558465 6_4:+75547586 None:intergenic
TGTGTTTCCGTTGATTGCTA+CGG 0.558738 6_4:-75545677 Msa0996830:three_prime_UTR
GAATGACTATTCAGGAAAGC+GGG 0.558880 6_4:-75546734 Msa0996830:intron
GAAACAATTCCAATGCGCCC+AGG 0.559949 6_4:+75548189 None:intergenic
ACGGAAACACAAACACTGGC+AGG 0.563800 6_4:+75545689 None:intergenic
CGTGTCCCATACGACGGCCA+GGG 0.564279 6_4:+75546667 None:intergenic
ATTCTTATTGGAGAGATAGG+AGG 0.566079 6_4:-75547344 Msa0996830:CDS
GAATATGGAACGAATATGGT+TGG 0.568288 6_4:-75549911 Msa0996830:CDS
GATAGGAGGAACTGCAGAAG+AGG 0.574249 6_4:-75547330 Msa0996830:CDS
AAAGAATAGTCAGTCCAGAA+AGG 0.575032 6_4:-75550195 Msa0996830:five_prime_UTR
GTTTACCAAACAACTGCTGT+TGG 0.576485 6_4:-75547645 Msa0996830:CDS
AGCACACGCGTGATCTGTCA+AGG 0.576838 6_4:-75549977 Msa0996830:CDS
TTGATAGGACCAAACTGCCC+TGG 0.579975 6_4:-75548267 Msa0996830:CDS
CGGGTTACATTCACAAACCT+GGG 0.586223 6_4:-75548206 Msa0996830:CDS
CCAGGTTTGTGAATGTAACC+CGG 0.592655 6_4:+75548207 None:intergenic
GGAAGCTGGAGTCACAGTTG+TGG 0.594495 6_4:-75546085 Msa0996830:CDS
GACGAACACCGCCGGAGAGG+GGG 0.599014 6_4:+75550006 None:intergenic
CAGTCCAGAAAGGAGGCACA+GGG 0.603456 6_4:-75550185 Msa0996830:five_prime_UTR
GGCCGTCGTATGGGACACGC+TGG 0.604310 6_4:-75546663 Msa0996830:CDS
ATCAACGGAAACACAAACAC+TGG 0.604639 6_4:+75545685 None:intergenic
CCACACATGTGGATTGTCCA+AGG 0.608972 6_4:+75547622 None:intergenic
CTTCTGTTTGAGGATCTGCT+AGG 0.613418 6_4:+75547544 None:intergenic
CATGTATCCGTAGCAATCAA+CGG 0.618935 6_4:+75545670 None:intergenic
TGTGACTCCAGCTTCCCTAA+GGG 0.620490 6_4:+75546092 None:intergenic
CTAGCAGATCCTCAAACAGA+AGG 0.626606 6_4:-75547543 Msa0996830:intron
TGGAACTTTGACATATGAAG+CGG 0.628520 6_4:-75548160 Msa0996830:intron
CGACGAACACCGCCGGAGAG+GGG 0.629139 6_4:+75550005 None:intergenic
GAATAGTCAGTCCAGAAAGG+AGG 0.635752 6_4:-75550192 Msa0996830:five_prime_UTR
CGTCGTATGGGACACGCTGG+AGG 0.636732 6_4:-75546660 Msa0996830:intron
ATAGCTACCATATCATGTTG+AGG 0.638728 6_4:+75549698 None:intergenic
TGAATGACTATTCAGGAAAG+CGG 0.638917 6_4:-75546735 Msa0996830:intron
TATTATGGAAGCGGTTGAAG+CGG 0.641435 6_4:-75549756 Msa0996830:CDS
CCCTGGTATCATCAAGCCCG+GGG 0.644773 6_4:-75548250 Msa0996830:CDS
CTTGGACAATCCACATGTGT+GGG 0.646091 6_4:-75547621 Msa0996830:CDS
TACTCCGAAGAAAGGCGGAA+CGG 0.647259 6_4:-75549882 Msa0996830:CDS
TGTGCAGCTATTGTGTCGGG+TGG 0.647703 6_4:-75546144 Msa0996830:intron
CCTTGGACAATCCACATGTG+TGG 0.655816 6_4:-75547622 Msa0996830:CDS
GTGTCCCATACGACGGCCAG+GGG 0.661520 6_4:+75546668 None:intergenic
GTCGACGAACACCGCCGGAG+AGG 0.665918 6_4:+75550003 None:intergenic
CCCCGGGCTTGATGATACCA+GGG 0.670275 6_4:+75548250 None:intergenic
TATGGAACGAATATGGTTGG+TGG 0.673659 6_4:-75549908 Msa0996830:CDS
ATAGCAGCAGCAGCAAAAGG+AGG 0.675861 6_4:+75549776 None:intergenic
GGTGTTACTCCGAAGAAAGG+CGG 0.682944 6_4:-75549887 Msa0996830:CDS
GGGTTGATGAGAAGTTGCGG+CGG 0.716803 6_4:+75550026 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATACATGTAGTATGATAAT+TGG - chr6_4:75550188-75550207 Msa0996830:five_prime_UTR 25.0%
!!! TTCAATTTTTAGTGTCTGCA+AGG - chr6_4:75550099-75550118 Msa0996830:five_prime_UTR 30.0%
AAAGAATAGTCAGTCCAGAA+AGG - chr6_4:75545648-75545667 Msa0996830:three_prime_UTR 35.0%
!!! ATTTTTAGTGTCTGCAAGGT+TGG - chr6_4:75550103-75550122 Msa0996830:five_prime_UTR 35.0%
ATCAACGGAAACACAAACAC+TGG + chr6_4:75550161-75550180 None:intergenic 40.0%
! TGTGTTTCCGTTGATTGCTA+CGG - chr6_4:75550166-75550185 Msa0996830:five_prime_UTR 40.0%
CATGTATCCGTAGCAATCAA+CGG + chr6_4:75550176-75550195 None:intergenic 40.0%
GAATAGTCAGTCCAGAAAGG+AGG - chr6_4:75545651-75545670 Msa0996830:three_prime_UTR 45.0%
ACGGAAACACAAACACTGGC+AGG + chr6_4:75550157-75550176 None:intergenic 50.0%
GCCACAAGCTGGAGCTCAAT+TGG + chr6_4:75550031-75550050 None:intergenic 55.0%
TACAAACCAGCGCCACAAGC+TGG + chr6_4:75550042-75550061 None:intergenic 55.0%
TGAGCTCCAGCTTGTGGCGC+TGG - chr6_4:75550033-75550052 Msa0996830:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 75545644 75550221 75545644 ID=Msa0996830;Name=Msa0996830
chr6_4 mRNA 75545644 75550221 75545644 ID=Msa0996830-mRNA-1;Parent=Msa0996830;Name=Msa0996830-mRNA-1;_AED=0.03;_eAED=0.03;_QI=123|1|1|1|1|1|6|376|323
chr6_4 exon 75545644 75546159 75545644 ID=Msa0996830-mRNA-1:exon:14153;Parent=Msa0996830-mRNA-1
chr6_4 exon 75546661 75546742 75546661 ID=Msa0996830-mRNA-1:exon:14152;Parent=Msa0996830-mRNA-1
chr6_4 exon 75547310 75547371 75547310 ID=Msa0996830-mRNA-1:exon:14151;Parent=Msa0996830-mRNA-1
chr6_4 exon 75547544 75547667 75547544 ID=Msa0996830-mRNA-1:exon:14150;Parent=Msa0996830-mRNA-1
chr6_4 exon 75548161 75548331 75548161 ID=Msa0996830-mRNA-1:exon:14149;Parent=Msa0996830-mRNA-1
chr6_4 exon 75549706 75550221 75549706 ID=Msa0996830-mRNA-1:exon:14148;Parent=Msa0996830-mRNA-1
chr6_4 five_prime_UTR 75550099 75550221 75550099 ID=Msa0996830-mRNA-1:five_prime_utr;Parent=Msa0996830-mRNA-1
chr6_4 CDS 75549706 75550098 75549706 ID=Msa0996830-mRNA-1:cds;Parent=Msa0996830-mRNA-1
chr6_4 CDS 75548161 75548331 75548161 ID=Msa0996830-mRNA-1:cds;Parent=Msa0996830-mRNA-1
chr6_4 CDS 75547544 75547667 75547544 ID=Msa0996830-mRNA-1:cds;Parent=Msa0996830-mRNA-1
chr6_4 CDS 75547310 75547371 75547310 ID=Msa0996830-mRNA-1:cds;Parent=Msa0996830-mRNA-1
chr6_4 CDS 75546661 75546742 75546661 ID=Msa0996830-mRNA-1:cds;Parent=Msa0996830-mRNA-1
chr6_4 CDS 75546020 75546159 75546020 ID=Msa0996830-mRNA-1:cds;Parent=Msa0996830-mRNA-1
chr6_4 three_prime_UTR 75545644 75546019 75545644 ID=Msa0996830-mRNA-1:three_prime_utr;Parent=Msa0996830-mRNA-1
Gene Sequence

>Msa0996830

ATGACTTTCCACCGCGTAACAAAACTCTTCTCATCAATCGCCTCCAACAACCGCCGCAACTTCTCATCAACCCCCTCTCCGGCGGTGTTCGTCGACAAAAGCACACGCGTGATCTGTCAAGGAATAACCGGCAAAAACGGAACGTTTCACACCGAACAAGCGATCGAATATGGAACGAATATGGTTGGTGGTGTTACTCCGAAGAAAGGCGGAACGGAACATTTAGGGCTTCCGGTTTTCAATTCTGTTGCGGAAGCGAAAACTGAAACGAAAGCAAATGCTTCTGTTATTTATGTTCCTCCTCCTTTTGCTGCTGCTGCTATTATGGAAGCGGTTGAAGCGGAGTTGGATCTTGTTGTTTGTATTACTGAAGGGATTCCTCAACATGATATGGTACGAGTTAAAGCAGCACTTAACAGACAATCCAAAACTCGTTTGATAGGACCAAACTGCCCTGGTATCATCAAGCCCGGGGAATGTAAAATTGGAATCATGCCGGGTTACATTCACAAACCTGGGCGCATTGGAATTGTTTCTCGTTCTGGAACTTTGACATATGAAGCGGTTTACCAAACAACTGCTGTTGGCCTTGGACAATCCACATGTGTGGGCATTGGAGGAGACCCCTTCAATGGAACAAACTTTGTTGATTGCCTTACAAAGTTCCTAGCAGATCCTCAAACAGAAGGTATTATTCTTATTGGAGAGATAGGAGGAACTGCAGAAGAGGACGCTGCTGCTCTAATTAAGGAAAGCGGGACTGAGAAGCCTGTTGTTGCATTTATTGCTGGATTAACTGCTCCCCCTGGCCGTCGTATGGGACACGCTGGAGCTATTGTGTCGGGTGGCAAGGGTACTGCTCAAGACAAAATCAGCACCCTTAGGGAAGCTGGAGTCACAGTTGTGGAGTCCCCAGCTAAAATTGGAGCTGCCATGTTTGATATTTTCAAGCAGAAAGGCTTAGTGCAATAG

Protein sequence

>Msa0996830

MTFHRVTKLFSSIASNNRRNFSSTPSPAVFVDKSTRVICQGITGKNGTFHTEQAIEYGTNMVGGVTPKKGGTEHLGLPVFNSVAEAKTETKANASVIYVPPPFAAAAIMEAVEAELDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRIGIVSRSGTLTYEAVYQTTAVGLGQSTCVGIGGDPFNGTNFVDCLTKFLADPQTEGIILIGEIGGTAEEDAAALIKESGTEKPVVAFIAGLTAPPGRRMGHAGAIVSGGKGTAQDKISTLREAGVTVVESPAKIGAAMFDIFKQKGLVQ*