AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0576760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576760 MtrunA17_Chr8g0392011 99.197 249 2 0 1 249 1 249 3.87e-179 491
Msa0576760 MtrunA17_Chr8g0392031 85.526 152 21 1 1 151 1 152 1.89e-90 263
Msa0576760 MtrunA17_Chr8g0392021 88.506 87 10 0 163 249 2 88 4.50e-48 154
Msa0576760 MtrunA17_Chr7g0246831 26.613 248 175 3 1 241 3 250 2.28e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0576760 AT2G01770.1 83.122 237 40 0 9 245 10 246 4.11e-144 403

Find 99 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCCTCTGCAGCTGCTTA+TGG 0.219518 4_1:-80832659 Msa0576760:CDS
GCACTATACAATGCTCTTCT+TGG 0.231503 4_1:+80832881 None:intergenic
TTGATTGCTTTGCTTATCTT+TGG 0.233804 4_1:-80832752 Msa0576760:CDS
TTACGATACGAACCAGTTTC+TGG 0.248340 4_1:+80834403 None:intergenic
ATTTCAAGCCAAAAGTTAAA+TGG 0.274829 4_1:+80832507 None:intergenic
TTCATGAACCATTTAACTTT+TGG 0.275424 4_1:-80832515 Msa0576760:three_prime_UTR
ATTGCTATTGCTTACGTATT+GGG 0.289589 4_1:-80832851 Msa0576760:CDS
CCATTTGAAGAGGTGTTAAA+TGG 0.291388 4_1:-80832388 Msa0576760:three_prime_UTR
GCTTTGGAAACTGCTCTAAT+TGG 0.299231 4_1:-80832686 Msa0576760:CDS
CTCTGCAGCTGCTTATGGTT+TGG 0.327872 4_1:-80832654 Msa0576760:CDS
TGTGTTGTTGTGAAGGTATT+TGG 0.344429 4_1:-80834494 Msa0576760:intron
GTTACACATTAGGCTTTGTT+TGG 0.346431 4_1:-80834898 Msa0576760:five_prime_UTR
TCACCGGCAACAAGCCTATC+AGG 0.353963 4_1:-80832712 Msa0576760:CDS
CATGGAATGGGCGATCATTA+TGG 0.364434 4_1:-80832432 Msa0576760:three_prime_UTR
GGAATAGAGGCACATGAATA+TGG 0.366935 4_1:-80834274 Msa0576760:CDS
TGCTGAGGTGGCAGAAATAT+TGG 0.367269 4_1:-80834305 Msa0576760:CDS
GTTTCCAAAGCACTCCTGAT+AGG 0.367638 4_1:+80832698 None:intergenic
TTGGCATGGCAGGTTTGAGC+TGG 0.371854 4_1:-80832921 Msa0576760:intron
GCAGAAATATTGGCTGAGTA+TGG 0.383074 4_1:-80834295 Msa0576760:CDS
TACAATGCTCTTCTTGGATC+TGG 0.387148 4_1:+80832887 None:intergenic
GGCGGCGACTTCGGCGATGC+CGG 0.389084 4_1:+80834620 None:intergenic
CGCCGCCGGCGCCATCTCCA+TGG 0.394416 4_1:-80834604 Msa0576760:CDS
GCCCTTGCTGCCGGTCTCTC+TGG 0.397127 4_1:-80834675 Msa0576760:CDS
GGCTCTGTTTCTCAGGTTCA+AGG 0.398656 4_1:+80834790 None:intergenic
TATAACAACAGTTACACATT+AGG 0.402678 4_1:-80834908 Msa0576760:five_prime_UTR
ATGTTACTTGCGGTGGCAAC+TGG 0.405180 4_1:-80832565 Msa0576760:three_prime_UTR
CTTCTTTGATAGTTGATACA+TGG 0.407242 4_1:-80832450 Msa0576760:three_prime_UTR
TTGAGAAGGCTCTGTTTCTC+AGG 0.409645 4_1:+80834783 None:intergenic
GGCGCCATCTCCATGGGTCT+CGG 0.413142 4_1:-80834597 Msa0576760:CDS
AGAACAGCCTCTGATGCATT+AGG 0.414520 4_1:+80832794 None:intergenic
GGCATCGCCGAAGTCGCCGC+CGG 0.418402 4_1:-80834618 Msa0576760:CDS
GATGGCGCCGGCGGCGACTT+CGG 0.423405 4_1:+80834611 None:intergenic
ATCGTTACAGTACCAGAAAC+TGG 0.426709 4_1:-80834415 Msa0576760:intron
AGCATTGGCGCCAGAGAGAC+CGG 0.431669 4_1:+80834665 None:intergenic
AATTGCTATTGCTTACGTAT+TGG 0.433881 4_1:-80832852 Msa0576760:CDS
ATTACAATAGAGAATTGAAA+AGG 0.434289 4_1:-80834450 Msa0576760:CDS
TCTGAAAGAGCATGAAACTA+TGG 0.439000 4_1:+80832617 None:intergenic
ATCACATACACTTGTTCAAA+TGG 0.440123 4_1:-80834841 Msa0576760:exon
CCATTTAACACCTCTTCAAA+TGG 0.440813 4_1:+80832388 None:intergenic
ACTCCTGATAGGCTTGTTGC+CGG 0.441410 4_1:+80832709 None:intergenic
TTGATAGTTGATACATGGAA+TGG 0.442334 4_1:-80832445 Msa0576760:three_prime_UTR
GCTTATCTTTGGCTTTGTCA+AGG 0.444850 4_1:-80832741 Msa0576760:CDS
CTTATCTTTGGCTTTGTCAA+GGG 0.448135 4_1:-80832740 Msa0576760:CDS
TTTGTCAAGGGATGCTTCAC+CGG 0.449841 4_1:-80832728 Msa0576760:CDS
GTTCCTTTCGCCCTTGCTGC+CGG 0.452907 4_1:-80834684 Msa0576760:CDS
CAAGCCTATCAGGAGTGCTT+TGG 0.453512 4_1:-80832702 Msa0576760:CDS
TGAAGGTATTTGGCAGCAAA+AGG 0.474070 4_1:-80834484 Msa0576760:intron
GCCAGAGAGACCGGCAGCAA+GGG 0.482759 4_1:+80834674 None:intergenic
TCTTCCATCGTTCTCACTGC+CGG 0.485751 4_1:-80834639 Msa0576760:CDS
GGCTGTTCTTGTTTCAGTGG+TGG 0.490681 4_1:-80832780 Msa0576760:CDS
ATCATCATCGGCGTTTCTGA+TGG 0.491623 4_1:-80834714 Msa0576760:CDS
AGCTGCTTATGGTTTGGCAA+AGG 0.497745 4_1:-80832648 Msa0576760:CDS
CTTGTTCAAATGGCTGGTAA+TGG 0.501587 4_1:-80834831 Msa0576760:exon
AGAGGCTGTTCTTGTTTCAG+TGG 0.506618 4_1:-80832783 Msa0576760:CDS
ACCTCTTCAAATGGTGGCGG+CGG 0.506745 4_1:+80832397 None:intergenic
AGACCGGCAGCAAGGGCGAA+AGG 0.507344 4_1:+80834681 None:intergenic
AAATGGCTATTGTTACAAGA+TGG 0.507486 4_1:-80832371 Msa0576760:three_prime_UTR
GCCGCCGGCGCCATCTCCAT+GGG 0.507839 4_1:-80834603 Msa0576760:CDS
ATTGGCTGAGTATGGAATAG+AGG 0.509860 4_1:-80834287 Msa0576760:CDS
TGATAGTTGATACATGGAAT+GGG 0.510160 4_1:-80832444 Msa0576760:three_prime_UTR
TGGCATGGCAGGTTTGAGCT+GGG 0.517647 4_1:-80832920 Msa0576760:intron
ACCCATGGAGATGGCGCCGG+CGG 0.521241 4_1:+80834602 None:intergenic
GCATTAGGAATGAACATGTA+AGG 0.527058 4_1:+80832809 None:intergenic
CATACACTTGTTCAAATGGC+TGG 0.531327 4_1:-80834837 Msa0576760:exon
AACGATGGAAGAAGTAGCAT+TGG 0.541787 4_1:+80834650 None:intergenic
CGCCAGAGAGACCGGCAGCA+AGG 0.541917 4_1:+80834673 None:intergenic
GGCAGCAAGGGCGAAAGGAA+CGG 0.542900 4_1:+80834686 None:intergenic
GCCGCCGCCACCATTTGAAG+AGG 0.547229 4_1:-80832398 Msa0576760:three_prime_UTR
TTACAATAGAGAATTGAAAA+GGG 0.552550 4_1:-80834449 Msa0576760:CDS
TTTCTCAGAGCATTCACCAC+AGG 0.554204 4_1:+80834250 None:intergenic
TGTATCAACTATCAAAGAAG+AGG 0.555184 4_1:+80832453 None:intergenic
AACACCTCTTCAAATGGTGG+CGG 0.559350 4_1:+80832394 None:intergenic
ATAAGCAGCTGCAGAGGCAA+TGG 0.561549 4_1:+80832661 None:intergenic
TTTCAGTGTGGCGATTGAGA+AGG 0.562660 4_1:+80834769 None:intergenic
CCCTCCGAGACCCATGGAGA+TGG 0.565170 4_1:+80834593 None:intergenic
TATTTCAGAAGCTGCTGAGG+TGG 0.571673 4_1:-80834317 Msa0576760:intron
TGTCTATTTGATGTTACTTG+CGG 0.578506 4_1:-80832575 Msa0576760:three_prime_UTR
GCTATTGCTTACGTATTGGG+TGG 0.579839 4_1:-80832848 Msa0576760:CDS
GAGACCCATGGAGATGGCGC+CGG 0.581337 4_1:+80834599 None:intergenic
TTAAATGGTTCATGAAAATG+TGG 0.587834 4_1:+80832522 None:intergenic
CCATCTCCATGGGTCTCGGA+GGG 0.588120 4_1:-80834593 Msa0576760:intron
TATTATTTCAGAAGCTGCTG+AGG 0.589707 4_1:-80834320 Msa0576760:intron
ACTGAAAAGCACTTCACCGC+CGG 0.593901 4_1:-80834753 Msa0576760:CDS
GATGTCACGGACGATCTCAC+CGG 0.594283 4_1:+80834734 None:intergenic
ATGAACATGTAAGGAATGAG+AGG 0.596128 4_1:+80832818 None:intergenic
AAAGAGCATGAAACTATGGA+TGG 0.598379 4_1:+80832621 None:intergenic
GTGTTACCCTCCGAGACCCA+TGG 0.602772 4_1:+80834587 None:intergenic
CTATTTGATGTTACTTGCGG+TGG 0.606313 4_1:-80832572 Msa0576760:three_prime_UTR
TTTAACACCTCTTCAAATGG+TGG 0.612867 4_1:+80832391 None:intergenic
TGAGAAAGAATCCTCAAGCA+TGG 0.613689 4_1:-80834234 Msa0576760:CDS
GCCATCTCCATGGGTCTCGG+AGG 0.615331 4_1:-80834594 Msa0576760:CDS
CAAACCATAAGCAGCTGCAG+AGG 0.616863 4_1:+80832655 None:intergenic
GTTCATTCCTAATGCATCAG+AGG 0.624017 4_1:-80832801 Msa0576760:CDS
ATGAAATCAAGCCATGCTTG+AGG 0.632968 4_1:+80834223 None:intergenic
ATCGTCCGTGACATCATCAT+CGG 0.638810 4_1:-80834726 Msa0576760:CDS
AAACGCCGATGATGATGTCA+CGG 0.645259 4_1:+80834721 None:intergenic
GTCACGGACGATCTCACCGG+CGG 0.688191 4_1:+80834737 None:intergenic
GGCACATGAATATGGACCTG+TGG 0.703794 4_1:-80834266 Msa0576760:CDS
GATGCCGGCAGTGAGAACGA+TGG 0.747818 4_1:+80834635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTATAGAAAAAGTTTT+CGG + chr4_1:80833464-80833483 None:intergenic 10.0%
!! TTATTAAAATAAGATTGATA+TGG - chr4_1:80833977-80833996 Msa0576760:intron 10.0%
!! TATGATAAAAATTGAAATTG+AGG + chr4_1:80833576-80833595 None:intergenic 15.0%
!! TATAAGTAAATAAATATCTC+TGG - chr4_1:80833856-80833875 Msa0576760:intron 15.0%
!! TCTTCATAGTATTAAAAAAA+TGG + chr4_1:80834063-80834082 None:intergenic 15.0%
!! GAACAAATTAATTAACAAAT+TGG - chr4_1:80834339-80834358 Msa0576760:intron 15.0%
!! ATTACAATAGAGAATTGAAA+AGG - chr4_1:80832837-80832856 Msa0576760:CDS 20.0%
!! TTACAATAGAGAATTGAAAA+GGG - chr4_1:80832838-80832857 Msa0576760:CDS 20.0%
!!! AAAGTAATTTGTCCAAAATT+TGG - chr4_1:80833146-80833165 Msa0576760:intron 20.0%
!! AATTTGTGTGAAGATAAAAT+AGG - chr4_1:80833533-80833552 Msa0576760:intron 20.0%
!!! GGTTTGGAATAAAAAAAAAA+AGG + chr4_1:80833600-80833619 None:intergenic 20.0%
!!! TGTTTTGAAAAACGTTTTTT+AGG + chr4_1:80833904-80833923 None:intergenic 20.0%
!!! TACTTTAGAAATCAAATTAG+AGG - chr4_1:80834002-80834021 Msa0576760:intron 20.0%
!!! GTATTCTAGAAACAATAAAA+AGG + chr4_1:80834249-80834268 None:intergenic 20.0%
!!! GAAATTAATTCACGTTTTTA+AGG + chr4_1:80834278-80834297 None:intergenic 20.0%
!! TAATTAACAAATTGGTTACT+TGG - chr4_1:80834347-80834366 Msa0576760:intron 20.0%
! TATAACAACAGTTACACATT+AGG - chr4_1:80832379-80832398 Msa0576760:three_prime_UTR 25.0%
!!! CTAGAAAGTGATTTTAGAAT+TGG + chr4_1:80832418-80832437 None:intergenic 25.0%
!! GATGTTAATTTAAAGTTGTG+TGG + chr4_1:80833415-80833434 None:intergenic 25.0%
! GAAGATAAAATAGGTAAACA+AGG - chr4_1:80833542-80833561 Msa0576760:intron 25.0%
! TAATATTAAGCATATGCTAC+TGG - chr4_1:80833684-80833703 Msa0576760:intron 25.0%
!!! ATTTTTTTGTACAGCTAGTT+AGG - chr4_1:80833785-80833804 Msa0576760:intron 25.0%
! TAAATAAATATCTCTGGTCT+TGG - chr4_1:80833862-80833881 Msa0576760:intron 25.0%
!! ATGAATATAAGTTGGATTCA+TGG + chr4_1:80834098-80834117 None:intergenic 25.0%
! ATGTAGAAACAATATAAAGG+TGG + chr4_1:80834121-80834140 None:intergenic 25.0%
! TGCATGTAGAAACAATATAA+AGG + chr4_1:80834124-80834143 None:intergenic 25.0%
! AAGGTGCATGTATATAAAAT+TGG + chr4_1:80834230-80834249 None:intergenic 25.0%
! ACGTGAATTAATTTCTACAA+TGG - chr4_1:80834283-80834302 Msa0576760:CDS 25.0%
! TTAAATGGTTCATGAAAATG+TGG + chr4_1:80834768-80834787 None:intergenic 25.0%
!!! TTCATGAACCATTTAACTTT+TGG - chr4_1:80834772-80834791 Msa0576760:CDS 25.0%
! ATTTCAAGCCAAAAGTTAAA+TGG + chr4_1:80834783-80834802 None:intergenic 25.0%
ATCACATACACTTGTTCAAA+TGG - chr4_1:80832446-80832465 Msa0576760:three_prime_UTR 30.0%
GTTGAATGTTAGTTATATGC+TGG - chr4_1:80833191-80833210 Msa0576760:intron 30.0%
TGATTTCAGATTCCAAACTT+TGG - chr4_1:80833295-80833314 Msa0576760:intron 30.0%
AAAGAGAGTCCAAATAGTAT+CGG + chr4_1:80833340-80833359 None:intergenic 30.0%
GATTCTACAATACAATGGTT+TGG + chr4_1:80833616-80833635 None:intergenic 30.0%
!! ACATCGATTCTACAATACAA+TGG + chr4_1:80833621-80833640 None:intergenic 30.0%
! TTTTATCCGTTTGCATTTCA+TGG + chr4_1:80833711-80833730 None:intergenic 30.0%
ATGAATAAATGATCATGTGC+AGG - chr4_1:80833808-80833827 Msa0576760:intron 30.0%
AATTAGAGGTAGAGATTTAC+TGG - chr4_1:80834016-80834035 Msa0576760:intron 30.0%
AAAGGTGGATGAATATAAGT+TGG + chr4_1:80834106-80834125 None:intergenic 30.0%
! AATTGCTATTGCTTACGTAT+TGG - chr4_1:80834435-80834454 Msa0576760:CDS 30.0%
! ATTGCTATTGCTTACGTATT+GGG - chr4_1:80834436-80834455 Msa0576760:CDS 30.0%
TTGATTGCTTTGCTTATCTT+TGG - chr4_1:80834535-80834554 Msa0576760:intron 30.0%
! TGTCTATTTGATGTTACTTG+CGG - chr4_1:80834712-80834731 Msa0576760:CDS 30.0%
!!! CTTTTGGCTTGAAATTTTTG+TGG - chr4_1:80834788-80834807 Msa0576760:CDS 30.0%
TGTATCAACTATCAAAGAAG+AGG + chr4_1:80834837-80834856 None:intergenic 30.0%
! CTTCTTTGATAGTTGATACA+TGG - chr4_1:80834837-80834856 Msa0576760:exon 30.0%
! TTGATAGTTGATACATGGAA+TGG - chr4_1:80834842-80834861 Msa0576760:exon 30.0%
! TGATAGTTGATACATGGAAT+GGG - chr4_1:80834843-80834862 Msa0576760:exon 30.0%
AAATGGCTATTGTTACAAGA+TGG - chr4_1:80834916-80834935 Msa0576760:five_prime_UTR 30.0%
!! GTTACACATTAGGCTTTGTT+TGG - chr4_1:80832389-80832408 Msa0576760:three_prime_UTR 35.0%
!! TGTGTTGTTGTGAAGGTATT+TGG - chr4_1:80832793-80832812 Msa0576760:CDS 35.0%
TATTATTTCAGAAGCTGCTG+AGG - chr4_1:80832967-80832986 Msa0576760:intron 35.0%
! GCATTGCAAACTCCAAATTT+TGG + chr4_1:80833161-80833180 None:intergenic 35.0%
!!! AAAGAGAAAGGCACTTTTTC+TGG - chr4_1:80833229-80833248 Msa0576760:intron 35.0%
ATAAAAGAAACCCACGTGTA+AGG + chr4_1:80833761-80833780 None:intergenic 35.0%
!! AAATATCTCTGGTCTTGGTT+TGG - chr4_1:80833867-80833886 Msa0576760:intron 35.0%
!! ATTCACGTTTTTAAGGAAGC+AGG + chr4_1:80834271-80834290 None:intergenic 35.0%
AACAAATTGGTTACTTGGCA+TGG - chr4_1:80834352-80834371 Msa0576760:intron 35.0%
ATGAACATGTAAGGAATGAG+AGG + chr4_1:80834472-80834491 None:intergenic 35.0%
GCATTAGGAATGAACATGTA+AGG + chr4_1:80834481-80834500 None:intergenic 35.0%
!! CTTATCTTTGGCTTTGTCAA+GGG - chr4_1:80834547-80834566 Msa0576760:intron 35.0%
! AAAGAGCATGAAACTATGGA+TGG + chr4_1:80834669-80834688 None:intergenic 35.0%
! TCTGAAAGAGCATGAAACTA+TGG + chr4_1:80834673-80834692 None:intergenic 35.0%
TTTAACACCTCTTCAAATGG+TGG + chr4_1:80834899-80834918 None:intergenic 35.0%
CCATTTAACACCTCTTCAAA+TGG + chr4_1:80834902-80834921 None:intergenic 35.0%
!! CCATTTGAAGAGGTGTTAAA+TGG - chr4_1:80834899-80834918 Msa0576760:five_prime_UTR 35.0%
CATACACTTGTTCAAATGGC+TGG - chr4_1:80832450-80832469 Msa0576760:three_prime_UTR 40.0%
CTTGTTCAAATGGCTGGTAA+TGG - chr4_1:80832456-80832475 Msa0576760:three_prime_UTR 40.0%
AACGATGGAAGAAGTAGCAT+TGG + chr4_1:80832640-80832659 None:intergenic 40.0%
!! TGAAGGTATTTGGCAGCAAA+AGG - chr4_1:80832803-80832822 Msa0576760:CDS 40.0%
ATCGTTACAGTACCAGAAAC+TGG - chr4_1:80832872-80832891 Msa0576760:CDS 40.0%
TTACGATACGAACCAGTTTC+TGG + chr4_1:80832887-80832906 None:intergenic 40.0%
! GCAGAAATATTGGCTGAGTA+TGG - chr4_1:80832992-80833011 Msa0576760:intron 40.0%
ATTGGCTGAGTATGGAATAG+AGG - chr4_1:80833000-80833019 Msa0576760:intron 40.0%
! GGAATAGAGGCACATGAATA+TGG - chr4_1:80833013-80833032 Msa0576760:intron 40.0%
TGAGAAAGAATCCTCAAGCA+TGG - chr4_1:80833053-80833072 Msa0576760:intron 40.0%
ATGAAATCAAGCCATGCTTG+AGG + chr4_1:80833067-80833086 None:intergenic 40.0%
TAGTTATATGCTGGCAAGAG+TGG - chr4_1:80833200-80833219 Msa0576760:intron 40.0%
AGTTATATGCTGGCAAGAGT+GGG - chr4_1:80833201-80833220 Msa0576760:intron 40.0%
!!! AAAGGCACTTTTTCTGGTTG+TGG - chr4_1:80833235-80833254 Msa0576760:intron 40.0%
!!! AAGGCACTTTTTCTGGTTGT+GGG - chr4_1:80833236-80833255 Msa0576760:intron 40.0%
!! CAAATAGTATCGGTGTACTG+TGG + chr4_1:80833330-80833349 None:intergenic 40.0%
CACAGTACACCGATACTATT+TGG - chr4_1:80833328-80833347 Msa0576760:intron 40.0%
ACTGGTCCATGAAATGCAAA+CGG - chr4_1:80833702-80833721 Msa0576760:intron 40.0%
TAGAGGTAGAGATTTACTGG+TGG - chr4_1:80834019-80834038 Msa0576760:intron 40.0%
TACAATGCTCTTCTTGGATC+TGG + chr4_1:80834403-80834422 None:intergenic 40.0%
GCACTATACAATGCTCTTCT+TGG + chr4_1:80834409-80834428 None:intergenic 40.0%
GTTCATTCCTAATGCATCAG+AGG - chr4_1:80834486-80834505 Msa0576760:intron 40.0%
!! GCTTATCTTTGGCTTTGTCA+AGG - chr4_1:80834546-80834565 Msa0576760:intron 40.0%
!! GCTTTGGAAACTGCTCTAAT+TGG - chr4_1:80834601-80834620 Msa0576760:CDS 40.0%
! CTATTTGATGTTACTTGCGG+TGG - chr4_1:80834715-80834734 Msa0576760:CDS 40.0%
! TTGAGAAGGCTCTGTTTCTC+AGG + chr4_1:80832507-80832526 None:intergenic 45.0%
TTTCAGTGTGGCGATTGAGA+AGG + chr4_1:80832521-80832540 None:intergenic 45.0%
ATCGTCCGTGACATCATCAT+CGG - chr4_1:80832561-80832580 Msa0576760:three_prime_UTR 45.0%
! AAACGCCGATGATGATGTCA+CGG + chr4_1:80832569-80832588 None:intergenic 45.0%
ATCATCATCGGCGTTTCTGA+TGG - chr4_1:80832573-80832592 Msa0576760:three_prime_UTR 45.0%
! GTTGCACTGTGTTGTTGTGA+AGG - chr4_1:80832786-80832805 Msa0576760:CDS 45.0%
TATTTCAGAAGCTGCTGAGG+TGG - chr4_1:80832970-80832989 Msa0576760:intron 45.0%
TGCTGAGGTGGCAGAAATAT+TGG - chr4_1:80832982-80833001 Msa0576760:intron 45.0%
! TTTCTCAGAGCATTCACCAC+AGG + chr4_1:80833040-80833059 None:intergenic 45.0%
GAGTGGGATTGGAAAGAGAA+AGG - chr4_1:80833217-80833236 Msa0576760:intron 45.0%
!!! GGGTTTCCACTTTTTCAGTG+TGG - chr4_1:80833256-80833275 Msa0576760:intron 45.0%
GCATGTCCACACTGAAAAAG+TGG + chr4_1:80833265-80833284 None:intergenic 45.0%
ATCCTAGAGAACCTTACACG+TGG - chr4_1:80833747-80833766 Msa0576760:intron 45.0%
TCCTAGAGAACCTTACACGT+GGG - chr4_1:80833748-80833767 Msa0576760:intron 45.0%
AATTGGTTACTTGGCATGGC+AGG - chr4_1:80834356-80834375 Msa0576760:intron 45.0%
GCTATTGCTTACGTATTGGG+TGG - chr4_1:80834439-80834458 Msa0576760:CDS 45.0%
AGAACAGCCTCTGATGCATT+AGG + chr4_1:80834496-80834515 None:intergenic 45.0%
! AGAGGCTGTTCTTGTTTCAG+TGG - chr4_1:80834504-80834523 Msa0576760:intron 45.0%
TTTGTCAAGGGATGCTTCAC+CGG - chr4_1:80834559-80834578 Msa0576760:intron 45.0%
! GTTTCCAAAGCACTCCTGAT+AGG + chr4_1:80834592-80834611 None:intergenic 45.0%
! AGCTGCTTATGGTTTGGCAA+AGG - chr4_1:80834639-80834658 Msa0576760:CDS 45.0%
CATGGAATGGGCGATCATTA+TGG - chr4_1:80834855-80834874 Msa0576760:five_prime_UTR 45.0%
AACACCTCTTCAAATGGTGG+CGG + chr4_1:80834896-80834915 None:intergenic 45.0%
!!! ATTTTATTTATTCTAAAAAA+TGG - chr4_1:80832913-80832932 Msa0576760:intron 5.0%
! GGCTCTGTTTCTCAGGTTCA+AGG + chr4_1:80832500-80832519 None:intergenic 50.0%
!! CGGTGAAGTGCTTTTCAGTG+TGG + chr4_1:80832533-80832552 None:intergenic 50.0%
!! ACTGAAAAGCACTTCACCGC+CGG - chr4_1:80832534-80832553 Msa0576760:three_prime_UTR 50.0%
TCTTCCATCGTTCTCACTGC+CGG - chr4_1:80832648-80832667 Msa0576760:CDS 50.0%
GGCACATGAATATGGACCTG+TGG - chr4_1:80833021-80833040 Msa0576760:intron 50.0%
TATGCTGGCAAGAGTGGGAT+TGG - chr4_1:80833206-80833225 Msa0576760:intron 50.0%
TGGCACGCATGACCAAAGTT+TGG + chr4_1:80833310-80833329 None:intergenic 50.0%
ACCCACGTGTAAGGTTCTCT+AGG + chr4_1:80833752-80833771 None:intergenic 50.0%
! GGCTGTTCTTGTTTCAGTGG+TGG - chr4_1:80834507-80834526 Msa0576760:intron 50.0%
!! ACTCCTGATAGGCTTGTTGC+CGG + chr4_1:80834581-80834600 None:intergenic 50.0%
!! CAAGCCTATCAGGAGTGCTT+TGG - chr4_1:80834585-80834604 Msa0576760:intron 50.0%
ATAAGCAGCTGCAGAGGCAA+TGG + chr4_1:80834629-80834648 None:intergenic 50.0%
ATTGCCTCTGCAGCTGCTTA+TGG - chr4_1:80834628-80834647 Msa0576760:CDS 50.0%
CAAACCATAAGCAGCTGCAG+AGG + chr4_1:80834635-80834654 None:intergenic 50.0%
CTCTGCAGCTGCTTATGGTT+TGG - chr4_1:80834633-80834652 Msa0576760:CDS 50.0%
! ATGTTACTTGCGGTGGCAAC+TGG - chr4_1:80834722-80834741 Msa0576760:CDS 50.0%
GATGTCACGGACGATCTCAC+CGG + chr4_1:80832556-80832575 None:intergenic 55.0%
TTGGCATGGCAGGTTTGAGC+TGG - chr4_1:80834366-80834385 Msa0576760:intron 55.0%
TGGCATGGCAGGTTTGAGCT+GGG - chr4_1:80834367-80834386 Msa0576760:intron 55.0%
TCACCGGCAACAAGCCTATC+AGG - chr4_1:80834575-80834594 Msa0576760:intron 55.0%
!! ACCTCTTCAAATGGTGGCGG+CGG + chr4_1:80834893-80834912 None:intergenic 55.0%
GGCAGCAAGGGCGAAAGGAA+CGG + chr4_1:80832604-80832623 None:intergenic 60.0%
GTTCCTTTCGCCCTTGCTGC+CGG - chr4_1:80832603-80832622 Msa0576760:three_prime_UTR 60.0%
!! AGCATTGGCGCCAGAGAGAC+CGG + chr4_1:80832625-80832644 None:intergenic 60.0%
GATGCCGGCAGTGAGAACGA+TGG + chr4_1:80832655-80832674 None:intergenic 60.0%
CCATCTCCATGGGTCTCGGA+GGG - chr4_1:80832694-80832713 Msa0576760:CDS 60.0%
GTGTTACCCTCCGAGACCCA+TGG + chr4_1:80832703-80832722 None:intergenic 60.0%
GTCACGGACGATCTCACCGG+CGG + chr4_1:80832553-80832572 None:intergenic 65.0%
AGACCGGCAGCAAGGGCGAA+AGG + chr4_1:80832609-80832628 None:intergenic 65.0%
GCCAGAGAGACCGGCAGCAA+GGG + chr4_1:80832616-80832635 None:intergenic 65.0%
GAGACCCATGGAGATGGCGC+CGG + chr4_1:80832691-80832710 None:intergenic 65.0%
GGCGCCATCTCCATGGGTCT+CGG - chr4_1:80832690-80832709 Msa0576760:CDS 65.0%
GCCATCTCCATGGGTCTCGG+AGG - chr4_1:80832693-80832712 Msa0576760:CDS 65.0%
CCCTCCGAGACCCATGGAGA+TGG + chr4_1:80832697-80832716 None:intergenic 65.0%
GCCGCCGCCACCATTTGAAG+AGG - chr4_1:80834889-80834908 Msa0576760:five_prime_UTR 65.0%
GCCCTTGCTGCCGGTCTCTC+TGG - chr4_1:80832612-80832631 Msa0576760:three_prime_UTR 70.0%
CGCCAGAGAGACCGGCAGCA+AGG + chr4_1:80832617-80832636 None:intergenic 70.0%
! ACCCATGGAGATGGCGCCGG+CGG + chr4_1:80832688-80832707 None:intergenic 70.0%
GGCGGCGACTTCGGCGATGC+CGG + chr4_1:80832670-80832689 None:intergenic 75.0%
GGCATCGCCGAAGTCGCCGC+CGG - chr4_1:80832669-80832688 Msa0576760:CDS 75.0%
!! GATGGCGCCGGCGGCGACTT+CGG + chr4_1:80832679-80832698 None:intergenic 75.0%
GCCGCCGGCGCCATCTCCAT+GGG - chr4_1:80832684-80832703 Msa0576760:CDS 75.0%
! CGCCGCCGGCGCCATCTCCA+TGG - chr4_1:80832683-80832702 Msa0576760:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 80832370 80834939 80832370 ID=Msa0576760;Name=Msa0576760
chr4_1 mRNA 80832370 80834939 80832370 ID=Msa0576760-mRNA-1;Parent=Msa0576760;Name=Msa0576760-mRNA-1;_AED=0.06;_eAED=0.06;_QI=95|1|1|1|1|1|4|264|249
chr4_1 exon 80832370 80832931 80832370 ID=Msa0576760-mRNA-1:exon:29058;Parent=Msa0576760-mRNA-1
chr4_1 exon 80834217 80834331 80834217 ID=Msa0576760-mRNA-1:exon:29057;Parent=Msa0576760-mRNA-1
chr4_1 exon 80834416 80834501 80834416 ID=Msa0576760-mRNA-1:exon:29056;Parent=Msa0576760-mRNA-1
chr4_1 exon 80834594 80834939 80834594 ID=Msa0576760-mRNA-1:exon:29055;Parent=Msa0576760-mRNA-1
chr4_1 five_prime_UTR 80834845 80834939 80834845 ID=Msa0576760-mRNA-1:five_prime_utr;Parent=Msa0576760-mRNA-1
chr4_1 CDS 80834594 80834844 80834594 ID=Msa0576760-mRNA-1:cds;Parent=Msa0576760-mRNA-1
chr4_1 CDS 80834416 80834501 80834416 ID=Msa0576760-mRNA-1:cds;Parent=Msa0576760-mRNA-1
chr4_1 CDS 80834217 80834331 80834217 ID=Msa0576760-mRNA-1:cds;Parent=Msa0576760-mRNA-1
chr4_1 CDS 80832634 80832931 80832634 ID=Msa0576760-mRNA-1:cds;Parent=Msa0576760-mRNA-1
chr4_1 three_prime_UTR 80832370 80832633 80832370 ID=Msa0576760-mRNA-1:three_prime_utr;Parent=Msa0576760-mRNA-1
Gene Sequence

>Msa0576760

ATGGCTGGTAATGGCGACGAACACAGACTAAACCTTGAACCTGAGAAACAGAGCCTTCTCAATCGCCACACTGAAAAGCACTTCACCGCCGGTGAGATCGTCCGTGACATCATCATCGGCGTTTCTGATGGACTCACCGTTCCTTTCGCCCTTGCTGCCGGTCTCTCTGGCGCCAATGCTACTTCTTCCATCGTTCTCACTGCCGGCATCGCCGAAGTCGCCGCCGGCGCCATCTCCATGGGTCTCGGAGGGTATTTGGCAGCAAAAGGTGAAGCTGATCATTACAATAGAGAATTGAAAAGGGAACAAGAAGAAATCGTTACAGTACCAGAAACTGAAGCTGCTGAGGTGGCAGAAATATTGGCTGAGTATGGAATAGAGGCACATGAATATGGACCTGTGGTGAATGCTCTGAGAAAGAATCCTCAAGCATGGCTTGATTTCATGATGAAGTTTGAGCTGGGACTAGAGAAGCCAGATCCAAGAAGAGCATTGTATAGTGCAATGACAATTGCTATTGCTTACGTATTGGGTGGAATTGTTCCTCTCATTCCTTACATGTTCATTCCTAATGCATCAGAGGCTGTTCTTGTTTCAGTGGTGGTTACATTGATTGCTTTGCTTATCTTTGGCTTTGTCAAGGGATGCTTCACCGGCAACAAGCCTATCAGGAGTGCTTTGGAAACTGCTCTAATTGGTGCCATTGCCTCTGCAGCTGCTTATGGTTTGGCAAAGGCTTTCCATCCATAG
Protein sequence

>Msa0576760

MAGNGDEHRLNLEPEKQSLLNRHTEKHFTAGEIVRDIIIGVSDGLTVPFALAAGLSGANATSSIVLTAGIAEVAAGAISMGLGGYLAAKGEADHYNRELKREQEEIVTVPETEAAEVAEILAEYGIEAHEYGPVVNALRKNPQAWLDFMMKFELGLEKPDPRRALYSAMTIAIAYVLGGIVPLIPYMFIPNASEAVLVSVVVTLIALLIFGFVKGCFTGNKPIRSALETALIGAIASAAAYGLAKAFHP*