AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0001880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0001880 MtrunA17_Chr8g0392011 99.197 249 2 0 1 249 1 249 3.87e-179 491
Msa0001880 MtrunA17_Chr8g0392031 85.526 152 21 1 1 151 1 152 1.89e-90 263
Msa0001880 MtrunA17_Chr8g0392021 88.506 87 10 0 163 249 2 88 4.50e-48 154
Msa0001880 MtrunA17_Chr7g0246831 26.613 248 175 3 1 241 3 250 2.28e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0001880 AT2G01770.1 83.122 237 40 0 9 245 10 246 4.11e-144 403

Find 98 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCCTCTGCAGCTGCTTA+TGG 0.219518 1_1:-3596213 Msa0001880:CDS
GCACTATACAATGCTCTTCT+TGG 0.231503 1_1:+3596435 None:intergenic
TTGATTGCTTTGCTTATCTT+TGG 0.233804 1_1:-3596306 Msa0001880:CDS
TTACGATACGAACCAGTTTC+TGG 0.248340 1_1:+3597962 None:intergenic
ATTTCAAGCCAAAAGTTAAA+TGG 0.274829 1_1:+3596061 None:intergenic
TTCATGAACCATTTAACTTT+TGG 0.275424 1_1:-3596069 Msa0001880:three_prime_UTR
ATTGCTATTGCTTACGTATT+GGG 0.289589 1_1:-3596405 Msa0001880:CDS
CCATTTGAAGAGGTGTTAAA+TGG 0.291388 1_1:-3595942 Msa0001880:three_prime_UTR
GCTTTGGAAACTGCTCTAAT+TGG 0.299231 1_1:-3596240 Msa0001880:CDS
CTCTGCAGCTGCTTATGGTT+TGG 0.327872 1_1:-3596208 Msa0001880:CDS
GTTACACATTAGGCTTTGTT+TGG 0.346431 1_1:-3598462 Msa0001880:five_prime_UTR
TCACCGGCAACAAGCCTATC+AGG 0.353963 1_1:-3596266 Msa0001880:CDS
GGAATAGAGGCACATGAATA+TGG 0.366935 1_1:-3597833 Msa0001880:CDS
TGCTGAGGTGGCAGAAATAT+TGG 0.367269 1_1:-3597864 Msa0001880:CDS
GTTTCCAAAGCACTCCTGAT+AGG 0.367638 1_1:+3596252 None:intergenic
TTGGCATGGCAGGTTTGAGC+TGG 0.371854 1_1:-3596475 Msa0001880:intron
GCAGAAATATTGGCTGAGTA+TGG 0.383074 1_1:-3597854 Msa0001880:CDS
TACAATGCTCTTCTTGGATC+TGG 0.387148 1_1:+3596441 None:intergenic
GGCGGCGACTTCGGCGATGC+CGG 0.389084 1_1:+3598184 None:intergenic
GCCCTCGCCGCCGGTCTCTC+TGG 0.390424 1_1:-3598239 Msa0001880:CDS
CGCCGCCGGCGCCATCTCCA+TGG 0.394416 1_1:-3598168 Msa0001880:CDS
GGCTCTGTTTCTCAGGTTCA+AGG 0.398656 1_1:+3598354 None:intergenic
TATAACAACAGTTACACATT+AGG 0.402678 1_1:-3598472 Msa0001880:five_prime_UTR
ATGTTACTTGCGGTGGCAAC+TGG 0.405180 1_1:-3596119 Msa0001880:three_prime_UTR
CTTCTTTGATAGTTGATACA+TGG 0.407242 1_1:-3596004 Msa0001880:three_prime_UTR
TTGAGAAGGCTCTGTTTCTC+AGG 0.409645 1_1:+3598347 None:intergenic
CATGGAATGAGCGATCATTA+TGG 0.412485 1_1:-3595986 Msa0001880:three_prime_UTR
TGTGTTGTCGTGAAGGTATT+TGG 0.412813 1_1:-3598053 Msa0001880:intron
GGCGCCATCTCCATGGGTCT+CGG 0.413142 1_1:-3598161 Msa0001880:CDS
AGAACAGCCTCTGATGCATT+AGG 0.414520 1_1:+3596348 None:intergenic
GGCATCGCCGAAGTCGCCGC+CGG 0.418402 1_1:-3598182 Msa0001880:CDS
GATGGCGCCGGCGGCGACTT+CGG 0.423405 1_1:+3598175 None:intergenic
ATCGTTACAGTACCAGAAAC+TGG 0.426709 1_1:-3597974 Msa0001880:intron
AGACCGGCGGCGAGGGCGAA+AGG 0.430567 1_1:+3598245 None:intergenic
AATTGCTATTGCTTACGTAT+TGG 0.433881 1_1:-3596406 Msa0001880:CDS
ATTACAATAGAGAATTGAAA+AGG 0.434289 1_1:-3598009 Msa0001880:CDS
TCTGAAAGAGCATGAAACTA+TGG 0.439000 1_1:+3596171 None:intergenic
ATCACATACACTTGTTCAAA+TGG 0.440123 1_1:-3598405 Msa0001880:exon
CCATTTAACACCTCTTCAAA+TGG 0.440813 1_1:+3595942 None:intergenic
ACTCCTGATAGGCTTGTTGC+CGG 0.441410 1_1:+3596263 None:intergenic
GCTTATCTTTGGCTTTGTCA+AGG 0.444850 1_1:-3596295 Msa0001880:CDS
CTTATCTTTGGCTTTGTCAA+GGG 0.448135 1_1:-3596294 Msa0001880:CDS
TTTGTCAAGGGATGCTTCAC+CGG 0.449841 1_1:-3596282 Msa0001880:CDS
CAAGCCTATCAGGAGTGCTT+TGG 0.453512 1_1:-3596256 Msa0001880:CDS
GTTCCTTTCGCCCTCGCCGC+CGG 0.459488 1_1:-3598248 Msa0001880:CDS
AGCATTGGCGCCAGAGAGAC+CGG 0.471916 1_1:+3598229 None:intergenic
GCCAGAGAGACCGGCGGCGA+GGG 0.479185 1_1:+3598238 None:intergenic
TCTTCCATCGTTCTCACTGC+CGG 0.485751 1_1:-3598203 Msa0001880:CDS
GGCTGTTCTTGTTTCAGTGG+TGG 0.490681 1_1:-3596334 Msa0001880:CDS
ATCATCATCGGCGTTTCTGA+TGG 0.491623 1_1:-3598278 Msa0001880:CDS
AGCTGCTTATGGTTTGGCAA+AGG 0.497745 1_1:-3596202 Msa0001880:CDS
CTTGTTCAAATGGCTGGTAA+TGG 0.501587 1_1:-3598395 Msa0001880:exon
TGAAGGTATTTGGCAGCAAA+AGG 0.504633 1_1:-3598043 Msa0001880:intron
AGAGGCTGTTCTTGTTTCAG+TGG 0.506618 1_1:-3596337 Msa0001880:CDS
ACCTCTTCAAATGGTGGCGG+CGG 0.506745 1_1:+3595951 None:intergenic
AAATGGCTATTGTTACAAGA+TGG 0.507486 1_1:-3595925 Msa0001880:three_prime_UTR
GCCGCCGGCGCCATCTCCAT+GGG 0.507839 1_1:-3598167 Msa0001880:CDS
ATTGGCTGAGTATGGAATAG+AGG 0.509860 1_1:-3597846 Msa0001880:CDS
TGGCATGGCAGGTTTGAGCT+GGG 0.517647 1_1:-3596474 Msa0001880:intron
ACCCATGGAGATGGCGCCGG+CGG 0.521241 1_1:+3598166 None:intergenic
GCATTAGGAATGAACATGTA+AGG 0.527058 1_1:+3596363 None:intergenic
CATACACTTGTTCAAATGGC+TGG 0.531327 1_1:-3598401 Msa0001880:exon
AACGATGGAAGAAGTAGCAT+TGG 0.541787 1_1:+3598214 None:intergenic
GCCGCCGCCACCATTTGAAG+AGG 0.547229 1_1:-3595952 Msa0001880:three_prime_UTR
TTACAATAGAGAATTGAAAA+GGG 0.552550 1_1:-3598008 Msa0001880:CDS
TTTCTCAGAGCATTCACCAC+AGG 0.554204 1_1:+3597809 None:intergenic
TGTATCAACTATCAAAGAAG+AGG 0.555184 1_1:+3596007 None:intergenic
GGCGGCGAGGGCGAAAGGAA+CGG 0.558595 1_1:+3598250 None:intergenic
AACACCTCTTCAAATGGTGG+CGG 0.559350 1_1:+3595948 None:intergenic
ATAAGCAGCTGCAGAGGCAA+TGG 0.561549 1_1:+3596215 None:intergenic
TTTCAGTGTGGCGATTGAGA+AGG 0.562660 1_1:+3598333 None:intergenic
CCCTCCGAGACCCATGGAGA+TGG 0.565170 1_1:+3598157 None:intergenic
CGCCAGAGAGACCGGCGGCG+AGG 0.570875 1_1:+3598237 None:intergenic
TATTTCAGAAGCTGCTGAGG+TGG 0.571673 1_1:-3597876 Msa0001880:intron
TGTCTATTTGATGTTACTTG+CGG 0.578506 1_1:-3596129 Msa0001880:three_prime_UTR
GCTATTGCTTACGTATTGGG+TGG 0.579839 1_1:-3596402 Msa0001880:CDS
GAGACCCATGGAGATGGCGC+CGG 0.581337 1_1:+3598163 None:intergenic
TTAAATGGTTCATGAAAATG+TGG 0.587834 1_1:+3596076 None:intergenic
CCATCTCCATGGGTCTCGGA+GGG 0.588120 1_1:-3598157 Msa0001880:intron
TATTATTTCAGAAGCTGCTG+AGG 0.589707 1_1:-3597879 Msa0001880:intron
ACTGAAAAGCACTTCACCGC+CGG 0.593901 1_1:-3598317 Msa0001880:CDS
GATGTCACGGACGATCTCAC+CGG 0.594283 1_1:+3598298 None:intergenic
ATGAACATGTAAGGAATGAG+AGG 0.596128 1_1:+3596372 None:intergenic
AAAGAGCATGAAACTATGGA+TGG 0.598379 1_1:+3596175 None:intergenic
GTGTTACCCTCCGAGACCCA+TGG 0.602772 1_1:+3598151 None:intergenic
CTATTTGATGTTACTTGCGG+TGG 0.606313 1_1:-3596126 Msa0001880:three_prime_UTR
TTTAACACCTCTTCAAATGG+TGG 0.612867 1_1:+3595945 None:intergenic
TGAGAAAGAATCCTCAAGCA+TGG 0.613689 1_1:-3597793 Msa0001880:CDS
GCCATCTCCATGGGTCTCGG+AGG 0.615331 1_1:-3598158 Msa0001880:CDS
CAAACCATAAGCAGCTGCAG+AGG 0.616863 1_1:+3596209 None:intergenic
GTTCATTCCTAATGCATCAG+AGG 0.624017 1_1:-3596355 Msa0001880:CDS
ATGAAATCAAGCCATGCTTG+AGG 0.632968 1_1:+3597782 None:intergenic
ATCGTCCGTGACATCATCAT+CGG 0.638810 1_1:-3598290 Msa0001880:CDS
AAACGCCGATGATGATGTCA+CGG 0.645259 1_1:+3598285 None:intergenic
GTCACGGACGATCTCACCGG+CGG 0.688191 1_1:+3598301 None:intergenic
GGCACATGAATATGGACCTG+TGG 0.703794 1_1:-3597825 Msa0001880:CDS
ATTGGCGCCAGAGAGACCGG+CGG 0.726652 1_1:+3598232 None:intergenic
GATGCCGGCAGTGAGAACGA+TGG 0.747818 1_1:+3598199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTATAGAAAAAGTTTT+CGG + chr1_1:3597030-3597049 None:intergenic 10.0%
!! TTATTAAAATAAGATTGATA+TGG - chr1_1:3597542-3597561 Msa0001880:intron 10.0%
!! TATGATAAAAATTGAAATTG+AGG + chr1_1:3597142-3597161 None:intergenic 15.0%
!! TATAAGTAAATAAATATCTC+TGG - chr1_1:3597421-3597440 Msa0001880:intron 15.0%
!! TTCTTCATAGTATTAAAAAA+AGG + chr1_1:3597628-3597647 None:intergenic 15.0%
!! GAACAAATTAATTAACAAAT+TGG - chr1_1:3597903-3597922 Msa0001880:intron 15.0%
!! ATTACAATAGAGAATTGAAA+AGG - chr1_1:3596396-3596415 Msa0001880:CDS 20.0%
!! TTACAATAGAGAATTGAAAA+GGG - chr1_1:3596397-3596416 Msa0001880:CDS 20.0%
!!! AAAGTAATTTGTCCAAAATT+TGG - chr1_1:3596705-3596724 Msa0001880:intron 20.0%
!! AATTTGTGTGAAGATAAAAT+AGG - chr1_1:3597099-3597118 Msa0001880:intron 20.0%
!!! TGGTTTGGAATAAAAAAAAA+TGG + chr1_1:3597166-3597185 None:intergenic 20.0%
!!! TGTTTTGAAAAACGTTTTTT+AGG + chr1_1:3597469-3597488 None:intergenic 20.0%
!!! TACTTTAGAAATCAAATTAG+AGG - chr1_1:3597567-3597586 Msa0001880:intron 20.0%
!!! GTATTCTAGAAACAATAAAA+AGG + chr1_1:3597813-3597832 None:intergenic 20.0%
!!! GAAATTAATTCACGTTTTTA+AGG + chr1_1:3597842-3597861 None:intergenic 20.0%
!! TAATTAACAAATTGGTTACT+TGG - chr1_1:3597911-3597930 Msa0001880:intron 20.0%
! TATAACAACAGTTACACATT+AGG - chr1_1:3595933-3595952 Msa0001880:three_prime_UTR 25.0%
!!! CTAGAAAGTGATTTTAGAAT+TGG + chr1_1:3595972-3595991 None:intergenic 25.0%
!! GATGTTAATTTAAAGTTGTG+TGG + chr1_1:3596981-3597000 None:intergenic 25.0%
! GAAGATAAAATAGGTAAACA+AGG - chr1_1:3597108-3597127 Msa0001880:intron 25.0%
!!! ACATTGATTCTACAATACAA+TGG + chr1_1:3597186-3597205 None:intergenic 25.0%
!!! ATTTTTTTGTACAGCTAGTT+AGG - chr1_1:3597350-3597369 Msa0001880:intron 25.0%
! TAAATAAATATCTCTGGTCT+TGG - chr1_1:3597427-3597446 Msa0001880:intron 25.0%
!! ATGAATATAAGTTGGATTCA+TGG + chr1_1:3597662-3597681 None:intergenic 25.0%
! TATATTCATCCACCTTTATA+TGG - chr1_1:3597673-3597692 Msa0001880:intron 25.0%
! AAGGTGCATGTATATAAAAT+TGG + chr1_1:3597794-3597813 None:intergenic 25.0%
! ACGTGAATTAATTTCTACAA+TGG - chr1_1:3597847-3597866 Msa0001880:CDS 25.0%
! TTAAATGGTTCATGAAAATG+TGG + chr1_1:3598332-3598351 None:intergenic 25.0%
!!! TTCATGAACCATTTAACTTT+TGG - chr1_1:3598336-3598355 Msa0001880:CDS 25.0%
! ATTTCAAGCCAAAAGTTAAA+TGG + chr1_1:3598347-3598366 None:intergenic 25.0%
ATCACATACACTTGTTCAAA+TGG - chr1_1:3596000-3596019 Msa0001880:three_prime_UTR 30.0%
GTTGAATGTTAGTTATATGC+TGG - chr1_1:3596752-3596771 Msa0001880:intron 30.0%
TGATTTCAGATTCCAAACTT+TGG - chr1_1:3596856-3596875 Msa0001880:intron 30.0%
GATTCTACAATACAATGGTT+TGG + chr1_1:3597181-3597200 None:intergenic 30.0%
TAATATTAAGCATATGCTCC+TGG - chr1_1:3597249-3597268 Msa0001880:intron 30.0%
! TTTTATCCGTTTGCATTTCA+TGG + chr1_1:3597276-3597295 None:intergenic 30.0%
ATGAATAAATGATCATGTGC+AGG - chr1_1:3597373-3597392 Msa0001880:intron 30.0%
AATTAGAGGTAGAGATTTAC+TGG - chr1_1:3597581-3597600 Msa0001880:intron 30.0%
AAAGGTGGATGAATATAAGT+TGG + chr1_1:3597670-3597689 None:intergenic 30.0%
ATGTAGAAACCATATAAAGG+TGG + chr1_1:3597685-3597704 None:intergenic 30.0%
TGCATGTAGAAACCATATAA+AGG + chr1_1:3597688-3597707 None:intergenic 30.0%
! AATTGCTATTGCTTACGTAT+TGG - chr1_1:3597999-3598018 Msa0001880:CDS 30.0%
! ATTGCTATTGCTTACGTATT+GGG - chr1_1:3598000-3598019 Msa0001880:CDS 30.0%
TTGATTGCTTTGCTTATCTT+TGG - chr1_1:3598099-3598118 Msa0001880:intron 30.0%
! TGTCTATTTGATGTTACTTG+CGG - chr1_1:3598276-3598295 Msa0001880:CDS 30.0%
!!! CTTTTGGCTTGAAATTTTTG+TGG - chr1_1:3598352-3598371 Msa0001880:CDS 30.0%
TGTATCAACTATCAAAGAAG+AGG + chr1_1:3598401-3598420 None:intergenic 30.0%
! CTTCTTTGATAGTTGATACA+TGG - chr1_1:3598401-3598420 Msa0001880:exon 30.0%
AAATGGCTATTGTTACAAGA+TGG - chr1_1:3598480-3598499 Msa0001880:five_prime_UTR 30.0%
!! GTTACACATTAGGCTTTGTT+TGG - chr1_1:3595943-3595962 Msa0001880:three_prime_UTR 35.0%
TATTATTTCAGAAGCTGCTG+AGG - chr1_1:3596526-3596545 Msa0001880:intron 35.0%
! GCATTGCAAACTCCAAATTT+TGG + chr1_1:3596720-3596739 None:intergenic 35.0%
!!! AAAGAGAAAGGCACTTTTTC+TGG - chr1_1:3596790-3596809 Msa0001880:intron 35.0%
TACACCGATACTATTTGGTT+TGG - chr1_1:3596894-3596913 Msa0001880:intron 35.0%
GAGTCCAAACCAAATAGTAT+CGG + chr1_1:3596901-3596920 None:intergenic 35.0%
ATAAAAGAAACCCACGTGTA+AGG + chr1_1:3597326-3597345 None:intergenic 35.0%
!! AAATATCTCTGGTCTTGGTT+TGG - chr1_1:3597432-3597451 Msa0001880:intron 35.0%
!! ATTCACGTTTTTAAGGAAGC+AGG + chr1_1:3597835-3597854 None:intergenic 35.0%
AACAAATTGGTTACTTGGCA+TGG - chr1_1:3597916-3597935 Msa0001880:intron 35.0%
ATGAACATGTAAGGAATGAG+AGG + chr1_1:3598036-3598055 None:intergenic 35.0%
GCATTAGGAATGAACATGTA+AGG + chr1_1:3598045-3598064 None:intergenic 35.0%
!! CTTATCTTTGGCTTTGTCAA+GGG - chr1_1:3598111-3598130 Msa0001880:intron 35.0%
! AAAGAGCATGAAACTATGGA+TGG + chr1_1:3598233-3598252 None:intergenic 35.0%
! TCTGAAAGAGCATGAAACTA+TGG + chr1_1:3598237-3598256 None:intergenic 35.0%
TTTAACACCTCTTCAAATGG+TGG + chr1_1:3598463-3598482 None:intergenic 35.0%
CCATTTAACACCTCTTCAAA+TGG + chr1_1:3598466-3598485 None:intergenic 35.0%
!! CCATTTGAAGAGGTGTTAAA+TGG - chr1_1:3598463-3598482 Msa0001880:five_prime_UTR 35.0%
CATACACTTGTTCAAATGGC+TGG - chr1_1:3596004-3596023 Msa0001880:three_prime_UTR 40.0%
CTTGTTCAAATGGCTGGTAA+TGG - chr1_1:3596010-3596029 Msa0001880:three_prime_UTR 40.0%
AACGATGGAAGAAGTAGCAT+TGG + chr1_1:3596194-3596213 None:intergenic 40.0%
ACTATGTTGTGTTGTCGTGA+AGG - chr1_1:3596345-3596364 Msa0001880:CDS 40.0%
!! TGTGTTGTCGTGAAGGTATT+TGG - chr1_1:3596352-3596371 Msa0001880:CDS 40.0%
!! TGAAGGTATTTGGCAGCAAA+AGG - chr1_1:3596362-3596381 Msa0001880:CDS 40.0%
ATCGTTACAGTACCAGAAAC+TGG - chr1_1:3596431-3596450 Msa0001880:CDS 40.0%
TTACGATACGAACCAGTTTC+TGG + chr1_1:3596446-3596465 None:intergenic 40.0%
! GCAGAAATATTGGCTGAGTA+TGG - chr1_1:3596551-3596570 Msa0001880:intron 40.0%
ATTGGCTGAGTATGGAATAG+AGG - chr1_1:3596559-3596578 Msa0001880:intron 40.0%
! GGAATAGAGGCACATGAATA+TGG - chr1_1:3596572-3596591 Msa0001880:intron 40.0%
TGAGAAAGAATCCTCAAGCA+TGG - chr1_1:3596612-3596631 Msa0001880:intron 40.0%
ATGAAATCAAGCCATGCTTG+AGG + chr1_1:3596626-3596645 None:intergenic 40.0%
TAGTTATATGCTGGCAAGAG+TGG - chr1_1:3596761-3596780 Msa0001880:intron 40.0%
AGTTATATGCTGGCAAGAGT+GGG - chr1_1:3596762-3596781 Msa0001880:intron 40.0%
!!! AAAGGCACTTTTTCTGGTTG+TGG - chr1_1:3596796-3596815 Msa0001880:intron 40.0%
!!! AAGGCACTTTTTCTGGTTGT+GGG - chr1_1:3596797-3596816 Msa0001880:intron 40.0%
!! CAAATAGTATCGGTGTACTG+TGG + chr1_1:3596891-3596910 None:intergenic 40.0%
CACAGTACACCGATACTATT+TGG - chr1_1:3596889-3596908 Msa0001880:intron 40.0%
TAGAGGTAGAGATTTACTGG+TGG - chr1_1:3597584-3597603 Msa0001880:intron 40.0%
TACAATGCTCTTCTTGGATC+TGG + chr1_1:3597967-3597986 None:intergenic 40.0%
GCACTATACAATGCTCTTCT+TGG + chr1_1:3597973-3597992 None:intergenic 40.0%
GTTCATTCCTAATGCATCAG+AGG - chr1_1:3598050-3598069 Msa0001880:intron 40.0%
!! GCTTATCTTTGGCTTTGTCA+AGG - chr1_1:3598110-3598129 Msa0001880:intron 40.0%
!! GCTTTGGAAACTGCTCTAAT+TGG - chr1_1:3598165-3598184 Msa0001880:CDS 40.0%
! CTATTTGATGTTACTTGCGG+TGG - chr1_1:3598279-3598298 Msa0001880:CDS 40.0%
CATGGAATGAGCGATCATTA+TGG - chr1_1:3598419-3598438 Msa0001880:five_prime_UTR 40.0%
! TTGAGAAGGCTCTGTTTCTC+AGG + chr1_1:3596061-3596080 None:intergenic 45.0%
TTTCAGTGTGGCGATTGAGA+AGG + chr1_1:3596075-3596094 None:intergenic 45.0%
ATCGTCCGTGACATCATCAT+CGG - chr1_1:3596115-3596134 Msa0001880:three_prime_UTR 45.0%
! AAACGCCGATGATGATGTCA+CGG + chr1_1:3596123-3596142 None:intergenic 45.0%
ATCATCATCGGCGTTTCTGA+TGG - chr1_1:3596127-3596146 Msa0001880:three_prime_UTR 45.0%
TATTTCAGAAGCTGCTGAGG+TGG - chr1_1:3596529-3596548 Msa0001880:intron 45.0%
TGCTGAGGTGGCAGAAATAT+TGG - chr1_1:3596541-3596560 Msa0001880:intron 45.0%
! TTTCTCAGAGCATTCACCAC+AGG + chr1_1:3596599-3596618 None:intergenic 45.0%
GAGTGGGATTGGAAAGAGAA+AGG - chr1_1:3596778-3596797 Msa0001880:intron 45.0%
!!! GGGTTTCCACTTTTTCAGTG+TGG - chr1_1:3596817-3596836 Msa0001880:intron 45.0%
GCATGTCCACACTGAAAAAG+TGG + chr1_1:3596826-3596845 None:intergenic 45.0%
CCTGGTCCATGAAATGCAAA+CGG - chr1_1:3597267-3597286 Msa0001880:intron 45.0%
ATCCTAGAGAACCTTACACG+TGG - chr1_1:3597312-3597331 Msa0001880:intron 45.0%
TCCTAGAGAACCTTACACGT+GGG - chr1_1:3597313-3597332 Msa0001880:intron 45.0%
AATTGGTTACTTGGCATGGC+AGG - chr1_1:3597920-3597939 Msa0001880:intron 45.0%
GCTATTGCTTACGTATTGGG+TGG - chr1_1:3598003-3598022 Msa0001880:CDS 45.0%
AGAACAGCCTCTGATGCATT+AGG + chr1_1:3598060-3598079 None:intergenic 45.0%
! AGAGGCTGTTCTTGTTTCAG+TGG - chr1_1:3598068-3598087 Msa0001880:intron 45.0%
TTTGTCAAGGGATGCTTCAC+CGG - chr1_1:3598123-3598142 Msa0001880:intron 45.0%
! GTTTCCAAAGCACTCCTGAT+AGG + chr1_1:3598156-3598175 None:intergenic 45.0%
! AGCTGCTTATGGTTTGGCAA+AGG - chr1_1:3598203-3598222 Msa0001880:CDS 45.0%
AACACCTCTTCAAATGGTGG+CGG + chr1_1:3598460-3598479 None:intergenic 45.0%
!!! ATTTTATTTATTCTAAAAAA+TGG - chr1_1:3596472-3596491 Msa0001880:intron 5.0%
! GGCTCTGTTTCTCAGGTTCA+AGG + chr1_1:3596054-3596073 None:intergenic 50.0%
!! CGGTGAAGTGCTTTTCAGTG+TGG + chr1_1:3596087-3596106 None:intergenic 50.0%
!! ACTGAAAAGCACTTCACCGC+CGG - chr1_1:3596088-3596107 Msa0001880:three_prime_UTR 50.0%
TCTTCCATCGTTCTCACTGC+CGG - chr1_1:3596202-3596221 Msa0001880:CDS 50.0%
GGCACATGAATATGGACCTG+TGG - chr1_1:3596580-3596599 Msa0001880:intron 50.0%
TATGCTGGCAAGAGTGGGAT+TGG - chr1_1:3596767-3596786 Msa0001880:intron 50.0%
TGGCACGCATGACCAAAGTT+TGG + chr1_1:3596871-3596890 None:intergenic 50.0%
CCGTTTGCATTTCATGGACC+AGG + chr1_1:3597270-3597289 None:intergenic 50.0%
ACCCACGTGTAAGGTTCTCT+AGG + chr1_1:3597317-3597336 None:intergenic 50.0%
! GGCTGTTCTTGTTTCAGTGG+TGG - chr1_1:3598071-3598090 Msa0001880:intron 50.0%
!! ACTCCTGATAGGCTTGTTGC+CGG + chr1_1:3598145-3598164 None:intergenic 50.0%
!! CAAGCCTATCAGGAGTGCTT+TGG - chr1_1:3598149-3598168 Msa0001880:intron 50.0%
ATAAGCAGCTGCAGAGGCAA+TGG + chr1_1:3598193-3598212 None:intergenic 50.0%
ATTGCCTCTGCAGCTGCTTA+TGG - chr1_1:3598192-3598211 Msa0001880:CDS 50.0%
CAAACCATAAGCAGCTGCAG+AGG + chr1_1:3598199-3598218 None:intergenic 50.0%
CTCTGCAGCTGCTTATGGTT+TGG - chr1_1:3598197-3598216 Msa0001880:CDS 50.0%
! ATGTTACTTGCGGTGGCAAC+TGG - chr1_1:3598286-3598305 Msa0001880:CDS 50.0%
GATGTCACGGACGATCTCAC+CGG + chr1_1:3596110-3596129 None:intergenic 55.0%
TTGGCATGGCAGGTTTGAGC+TGG - chr1_1:3597930-3597949 Msa0001880:intron 55.0%
TGGCATGGCAGGTTTGAGCT+GGG - chr1_1:3597931-3597950 Msa0001880:intron 55.0%
TCACCGGCAACAAGCCTATC+AGG - chr1_1:3598139-3598158 Msa0001880:intron 55.0%
!! ACCTCTTCAAATGGTGGCGG+CGG + chr1_1:3598457-3598476 None:intergenic 55.0%
!! AGCATTGGCGCCAGAGAGAC+CGG + chr1_1:3596179-3596198 None:intergenic 60.0%
GATGCCGGCAGTGAGAACGA+TGG + chr1_1:3596209-3596228 None:intergenic 60.0%
CCATCTCCATGGGTCTCGGA+GGG - chr1_1:3596248-3596267 Msa0001880:CDS 60.0%
GTGTTACCCTCCGAGACCCA+TGG + chr1_1:3596257-3596276 None:intergenic 60.0%
GTCACGGACGATCTCACCGG+CGG + chr1_1:3596107-3596126 None:intergenic 65.0%
ATTGGCGCCAGAGAGACCGG+CGG + chr1_1:3596176-3596195 None:intergenic 65.0%
GAGACCCATGGAGATGGCGC+CGG + chr1_1:3596245-3596264 None:intergenic 65.0%
GGCGCCATCTCCATGGGTCT+CGG - chr1_1:3596244-3596263 Msa0001880:CDS 65.0%
GCCATCTCCATGGGTCTCGG+AGG - chr1_1:3596247-3596266 Msa0001880:CDS 65.0%
CCCTCCGAGACCCATGGAGA+TGG + chr1_1:3596251-3596270 None:intergenic 65.0%
GCCGCCGCCACCATTTGAAG+AGG - chr1_1:3598453-3598472 Msa0001880:five_prime_UTR 65.0%
GGCGGCGAGGGCGAAAGGAA+CGG + chr1_1:3596158-3596177 None:intergenic 70.0%
GTTCCTTTCGCCCTCGCCGC+CGG - chr1_1:3596157-3596176 Msa0001880:three_prime_UTR 70.0%
! ACCCATGGAGATGGCGCCGG+CGG + chr1_1:3596242-3596261 None:intergenic 70.0%
AGACCGGCGGCGAGGGCGAA+AGG + chr1_1:3596163-3596182 None:intergenic 75.0%
GCCAGAGAGACCGGCGGCGA+GGG + chr1_1:3596170-3596189 None:intergenic 75.0%
GGCGGCGACTTCGGCGATGC+CGG + chr1_1:3596224-3596243 None:intergenic 75.0%
GGCATCGCCGAAGTCGCCGC+CGG - chr1_1:3596223-3596242 Msa0001880:CDS 75.0%
!! GATGGCGCCGGCGGCGACTT+CGG + chr1_1:3596233-3596252 None:intergenic 75.0%
GCCGCCGGCGCCATCTCCAT+GGG - chr1_1:3596238-3596257 Msa0001880:CDS 75.0%
! GCCCTCGCCGCCGGTCTCTC+TGG - chr1_1:3596166-3596185 Msa0001880:three_prime_UTR 80.0%
! CGCCAGAGAGACCGGCGGCG+AGG + chr1_1:3596171-3596190 None:intergenic 80.0%
! CGCCGCCGGCGCCATCTCCA+TGG - chr1_1:3596237-3596256 Msa0001880:CDS 80.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 3595924 3598503 3595924 ID=Msa0001880;Name=Msa0001880
chr1_1 mRNA 3595924 3598503 3595924 ID=Msa0001880-mRNA-1;Parent=Msa0001880;Name=Msa0001880-mRNA-1;_AED=0.06;_eAED=0.06;_QI=95|1|1|1|1|1|4|264|249
chr1_1 exon 3595924 3596485 3595924 ID=Msa0001880-mRNA-1:exon:1066;Parent=Msa0001880-mRNA-1
chr1_1 exon 3597776 3597890 3597776 ID=Msa0001880-mRNA-1:exon:1065;Parent=Msa0001880-mRNA-1
chr1_1 exon 3597975 3598060 3597975 ID=Msa0001880-mRNA-1:exon:1064;Parent=Msa0001880-mRNA-1
chr1_1 exon 3598158 3598503 3598158 ID=Msa0001880-mRNA-1:exon:1063;Parent=Msa0001880-mRNA-1
chr1_1 five_prime_UTR 3598409 3598503 3598409 ID=Msa0001880-mRNA-1:five_prime_utr;Parent=Msa0001880-mRNA-1
chr1_1 CDS 3598158 3598408 3598158 ID=Msa0001880-mRNA-1:cds;Parent=Msa0001880-mRNA-1
chr1_1 CDS 3597975 3598060 3597975 ID=Msa0001880-mRNA-1:cds;Parent=Msa0001880-mRNA-1
chr1_1 CDS 3597776 3597890 3597776 ID=Msa0001880-mRNA-1:cds;Parent=Msa0001880-mRNA-1
chr1_1 CDS 3596188 3596485 3596188 ID=Msa0001880-mRNA-1:cds;Parent=Msa0001880-mRNA-1
chr1_1 three_prime_UTR 3595924 3596187 3595924 ID=Msa0001880-mRNA-1:three_prime_utr;Parent=Msa0001880-mRNA-1
Gene Sequence

>Msa0001880

ATGGCTGGTAATGGCGACGAACACAGACTAAACCTTGAACCTGAGAAACAGAGCCTTCTCAATCGCCACACTGAAAAGCACTTCACCGCCGGTGAGATCGTCCGTGACATCATCATCGGCGTTTCTGATGGACTCACCGTTCCTTTCGCCCTCGCCGCCGGTCTCTCTGGCGCCAATGCTACTTCTTCCATCGTTCTCACTGCCGGCATCGCCGAAGTCGCCGCCGGCGCCATCTCCATGGGTCTCGGAGGGTATTTGGCAGCAAAAGGTGAAGCTGATCATTACAATAGAGAATTGAAAAGGGAACAAGAAGAAATCGTTACAGTACCAGAAACTGAAGCTGCTGAGGTGGCAGAAATATTGGCTGAGTATGGAATAGAGGCACATGAATATGGACCTGTGGTGAATGCTCTGAGAAAGAATCCTCAAGCATGGCTTGATTTCATGATGAAGTTTGAGCTGGGACTAGAGAAGCCAGATCCAAGAAGAGCATTGTATAGTGCAATGACAATTGCTATTGCTTACGTATTGGGTGGAATTGTTCCTCTCATTCCTTACATGTTCATTCCTAATGCATCAGAGGCTGTTCTTGTTTCAGTGGTGGTTACATTGATTGCTTTGCTTATCTTTGGCTTTGTCAAGGGATGCTTCACCGGCAACAAGCCTATCAGGAGTGCTTTGGAAACTGCTCTAATTGGTGCCATTGCCTCTGCAGCTGCTTATGGTTTGGCAAAGGCTTTCCATCCATAG
Protein sequence

>Msa0001880

MAGNGDEHRLNLEPEKQSLLNRHTEKHFTAGEIVRDIIIGVSDGLTVPFALAAGLSGANATSSIVLTAGIAEVAAGAISMGLGGYLAAKGEADHYNRELKREQEEIVTVPETEAAEVAEILAEYGIEAHEYGPVVNALRKNPQAWLDFMMKFELGLEKPDPRRALYSAMTIAIAYVLGGIVPLIPYMFIPNASEAVLVSVVVTLIALLIFGFVKGCFTGNKPIRSALETALIGAIASAAAYGLAKAFHP*