AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0603840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603840 MtrunA17_Chr4g0032621 97.861 187 4 0 8 194 1 187 1.83e-136 379
Msa0603840 MtrunA17_Chr2g0328941 74.332 187 33 2 8 194 1 172 2.28e-97 280
Msa0603840 MtrunA17_Chr7g0258071 57.297 185 63 3 10 194 92 260 1.87e-70 214
Msa0603840 MtrunA17_Chr1g0173971 55.676 185 63 3 10 194 4 169 4.28e-68 205
Msa0603840 MtrunA17_Chr7g0266121 41.477 176 86 4 19 194 10 168 4.71e-40 134
Msa0603840 MtrunA17_Chr4g0026561 39.674 184 95 4 11 194 3 170 3.12e-39 132
Msa0603840 MtrunA17_Chr2g0321261 39.884 173 72 4 22 194 14 154 8.71e-38 127
Msa0603840 MtrunA17_Chr1g0178851 42.703 185 74 6 11 194 3 156 1.14e-34 120
Msa0603840 MtrunA17_Chr8g0351971 56.164 73 32 0 122 194 168 240 3.64e-26 100
Msa0603840 MtrunA17_Chr3g0087831 59.016 61 25 0 134 194 103 163 1.65e-23 91.7
Msa0603840 MtrunA17_Chr1g0203591 30.814 172 82 5 22 192 3 138 9.48e-23 89.0
Msa0603840 MtrunA17_Chr7g0274001 30.000 170 77 4 22 191 3 130 3.31e-22 87.4
Msa0603840 MtrunA17_Chr2g0306911 30.409 171 70 4 22 192 4 125 1.84e-17 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603840 AT1G51200.4 59.893 187 61 4 8 194 1 173 3.24e-64 196
Msa0603840 AT1G51200.1 59.893 187 61 4 8 194 1 173 3.24e-64 196
Msa0603840 AT1G51200.3 59.893 187 61 4 8 194 1 173 3.24e-64 196
Msa0603840 AT1G51200.2 59.893 187 61 4 8 194 1 173 3.24e-64 196
Msa0603840 AT2G36320.1 41.758 182 70 4 19 194 10 161 2.35e-42 140
Msa0603840 AT2G27580.2 39.306 173 86 3 22 194 10 163 4.27e-39 132
Msa0603840 AT2G27580.1 39.306 173 86 3 22 194 10 163 4.27e-39 132
Msa0603840 AT4G12040.2 36.872 179 96 3 18 194 12 175 1.15e-38 131
Msa0603840 AT4G12040.1 36.872 179 96 3 18 194 12 175 1.15e-38 131
Msa0603840 AT1G12440.2 38.068 176 89 1 19 194 13 168 1.29e-37 128
Msa0603840 AT1G12440.1 38.068 176 89 1 19 194 13 168 1.29e-37 128
Msa0603840 AT1G12440.3 38.068 176 89 1 19 194 13 168 1.29e-37 128
Msa0603840 AT4G22820.1 36.994 173 94 2 22 194 19 176 1.13e-36 126
Msa0603840 AT4G22820.2 36.994 173 94 2 22 194 19 176 1.13e-36 126
Msa0603840 AT3G52800.2 42.021 188 85 5 11 194 3 170 5.43e-36 124
Msa0603840 AT3G52800.1 42.021 188 85 5 11 194 3 170 5.43e-36 124
Msa0603840 AT3G12630.1 36.747 166 68 5 30 194 31 160 1.36e-27 102
Msa0603840 AT4G14225.1 32.948 173 64 2 22 194 5 125 1.18e-25 96.3
Msa0603840 AT4G25380.1 28.736 174 67 3 25 194 10 130 3.94e-22 87.4

Find 80 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTTCCTGCCCATCTTAT+TGG 0.244242 4_2:+48643674 Msa0603840:three_prime_UTR
GGGATTGTTTGCAGGTATTC+AGG 0.258711 4_2:+48641265 None:intergenic
AAAGGCTTAGAGATAGTTTC+AGG 0.302295 4_2:-48643342 None:intergenic
GTATCTTTGCCCTAGTGTTT+AGG 0.317239 4_2:+48643791 Msa0603840:three_prime_UTR
AGCTTCTCAGCCTTGACAAC+CGG 0.320370 4_2:-48643573 None:intergenic
ATCTAAGGTTGTAAAACTTC+TGG 0.321036 4_2:-48643619 None:intergenic
TTCGGACCATCCTTCGGCTT+TGG 0.323240 4_2:-48643399 None:intergenic
ATGCACAATATAGGACCTTC+AGG 0.357857 4_2:-48643078 None:intergenic
GTTTCAGCATCATGTCTTTG+TGG 0.367335 4_2:-48643155 None:intergenic
AATAAGATGGGCAGGAAACT+TGG 0.368641 4_2:-48643672 None:intergenic
GGTACTTGGTCTTGGTCATA+TGG 0.372583 4_2:+48643725 Msa0603840:three_prime_UTR
CTTGACAACCGGGTTTGCTT+TGG 0.380130 4_2:-48643562 None:intergenic
TATGGAAATATCTGCAGTTT+GGG 0.385091 4_2:+48643743 Msa0603840:three_prime_UTR
AGTTTCAGGTTCCACAGAAA+TGG 0.392851 4_2:-48643328 None:intergenic
TTGCAGTTGCTGCAACGTTT+CGG 0.398329 4_2:-48643417 None:intergenic
ATATGGAAATATCTGCAGTT+TGG 0.401334 4_2:+48643742 Msa0603840:three_prime_UTR
GCTTAGATTGTATTTATTGT+GGG 0.412178 4_2:+48643931 Msa0603840:three_prime_UTR
TCCCTTGGAAATATTATGAA+TGG 0.415286 4_2:+48643204 Msa0603840:CDS
CAAAGACATGATGCTGAAAC+AGG 0.424095 4_2:+48643158 Msa0603840:CDS
AATAAGCGAGTTGGTTTGAC+AGG 0.426782 4_2:+48643438 Msa0603840:CDS
AATTGAGCATAACATATCTA+AGG 0.426869 4_2:-48643634 None:intergenic
TGCTTAGATTGTATTTATTG+TGG 0.430392 4_2:+48643930 Msa0603840:three_prime_UTR
TTTGTTATATTATATGTGGT+GGG 0.437083 4_2:+48643702 Msa0603840:three_prime_UTR
GCTTGATAAGATCTGAGAAT+TGG 0.443173 4_2:+48643593 Msa0603840:exon
CAACGTTTCGGACCATCCTT+CGG 0.445047 4_2:-48643405 None:intergenic
AACCGGGTTTGCTTTGGCTA+TGG 0.447808 4_2:-48643556 None:intergenic
AACAAAGAACACCAATAAGA+TGG 0.449850 4_2:-48643685 None:intergenic
CAGTTGTTGATGCACAATAT+AGG 0.453708 4_2:-48643087 None:intergenic
TGCACATACCAATCTGAAAC+GGG 0.457213 4_2:-48643870 None:intergenic
GATTATAGTTTGTAATCGAT+TGG 0.461995 4_2:+48643974 Msa0603840:three_prime_UTR
TTAGGTTCCACGGAAATGGC+TGG 0.467021 4_2:-48643282 None:intergenic
GTTCTTTGTTATATTATATG+TGG 0.469908 4_2:+48643698 Msa0603840:three_prime_UTR
TGTTATATTATATGTGGTGG+GGG 0.471509 4_2:+48643704 Msa0603840:three_prime_UTR
GCATCTTTCCCGTTTCAGAT+TGG 0.476182 4_2:+48643862 Msa0603840:three_prime_UTR
CTTTGTTATATTATATGTGG+TGG 0.480434 4_2:+48643701 Msa0603840:three_prime_UTR
TGTACTGGTTTGGTGGAAGT+TGG 0.485428 4_2:+48643824 Msa0603840:three_prime_UTR
GCACTTAGAGCACATGTTCA+TGG 0.487125 4_2:-48643133 None:intergenic
TTGTTATATTATATGTGGTG+GGG 0.495712 4_2:+48643703 Msa0603840:three_prime_UTR
CCCTTGGAAATATTATGAAT+GGG 0.495769 4_2:+48643205 Msa0603840:CDS
TTATATATGTACTGGTTTGG+TGG 0.498110 4_2:+48643817 Msa0603840:three_prime_UTR
AATACAATCTAAGCATATGC+TGG 0.499516 4_2:-48643922 None:intergenic
CAGAAGTGCGGTAATCGAAC+GGG 0.502252 4_2:-48643524 None:intergenic
GCTTCTCAGCCTTGACAACC+GGG 0.508433 4_2:-48643572 None:intergenic
AAGGGATGATGCTGCAAGAT+GGG 0.510002 4_2:-48643187 None:intergenic
ACGCTGGCAGCAGTAACAAC+AGG 0.516439 4_2:-48643252 None:intergenic
CCCATTCATAATATTTCCAA+GGG 0.520390 4_2:-48643205 None:intergenic
GTGCAGGAAATTTGAAACAA+TGG 0.525517 4_2:+48643026 Msa0603840:intron
ACCCATTCATAATATTTCCA+AGG 0.526039 4_2:-48643206 None:intergenic
TTGCACATACCAATCTGAAA+CGG 0.526576 4_2:-48643871 None:intergenic
CAAGGGATGATGCTGCAAGA+TGG 0.532070 4_2:-48643188 None:intergenic
TCAGGTTCCACAGAAATGGA+TGG 0.538028 4_2:-48643324 None:intergenic
AGCAACTGCAATAAGCGAGT+TGG 0.543723 4_2:+48643429 Msa0603840:CDS
AGCGATGTACAGCACAGAAA+AGG 0.543830 4_2:-48643482 None:intergenic
CATCTTGCAGCATCATCCCT+TGG 0.544464 4_2:+48643189 Msa0603840:CDS
CAAAGCAAACCCGGTTGTCA+AGG 0.546068 4_2:+48643563 Msa0603840:CDS
TGCCATAGCCAAAGCAAACC+CGG 0.547092 4_2:+48643554 Msa0603840:CDS
TTTATTTGGCTCAGGTTCAG+AGG 0.556901 4_2:+48643365 Msa0603840:CDS
AGAACACCAATAAGATGGGC+AGG 0.557839 4_2:-48643680 None:intergenic
GCAGAAGTGCGGTAATCGAA+CGG 0.558885 4_2:-48643525 None:intergenic
TAGGTTCCACGGAAATGGCT+GGG 0.559679 4_2:-48643281 None:intergenic
TGCACAATATAGGACCTTCA+GGG 0.559940 4_2:-48643077 None:intergenic
CAGGTTCCACAGAAATGGAT+GGG 0.560627 4_2:-48643323 None:intergenic
ACAAAGAACACCAATAAGAT+GGG 0.572088 4_2:-48643684 None:intergenic
ATAAGATGGGCAGGAAACTT+GGG 0.574435 4_2:-48643671 None:intergenic
CATGATGCTGAAACAGGAGC+AGG 0.593551 4_2:+48643164 Msa0603840:CDS
ACAGGGTTTAATTGTCGATG+TGG 0.597602 4_2:+48643456 Msa0603840:CDS
GGTGATCCAAAGCCGAAGGA+TGG 0.601061 4_2:+48643393 Msa0603840:CDS
CGATATCCCATCCATTTCTG+TGG 0.604057 4_2:+48643317 Msa0603840:CDS
AATGGATGGGATATCGACAC+TGG 0.619918 4_2:-48643310 None:intergenic
TGGCATCTCGTGCAGAAGTG+CGG 0.628982 4_2:-48643536 None:intergenic
GAGTGGTGATCCAAAGCCGA+AGG 0.630939 4_2:+48643389 Msa0603840:CDS
GGATATCCCAGCCATTTCCG+TGG 0.638474 4_2:+48643275 Msa0603840:CDS
GCACAATATAGGACCTTCAG+GGG 0.644318 4_2:-48643076 None:intergenic
ATAAGCGAGTTGGTTTGACA+GGG 0.651214 4_2:+48643439 Msa0603840:CDS
AATGGCTGGGATATCCACGC+TGG 0.652769 4_2:-48643268 None:intergenic
GGCTCAGGTTCAGAGGAGAG+TGG 0.659270 4_2:+48643372 Msa0603840:CDS
TGTTGTTACTGCTGCCAGCG+TGG 0.668169 4_2:+48643254 Msa0603840:CDS
ATATTGTGCATCAACAACTG+TGG 0.675543 4_2:+48643090 Msa0603840:CDS
ACGGGAAAGATGCATTACAA+AGG 0.677131 4_2:-48643852 None:intergenic
CACAATATAGGACCTTCAGG+GGG 0.733275 4_2:-48643075 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATGATAATTTAATCTT+TGG + chr4_2:48642996-48643015 Msa0603840:intron 10.0%
!! TTAATGATAATTTAATCTTT+GGG + chr4_2:48642997-48643016 Msa0603840:intron 10.0%
!!! TTCTTCTTTTTTTTTTTACT+TGG - chr4_2:48644027-48644046 None:intergenic 15.0%
!! GTAAAAAAAAAAAGAAGAAT+TGG + chr4_2:48644028-48644047 Msa0603840:three_prime_UTR 15.0%
!! AGGTTCACTTAAATTAAATT+TGG + chr4_2:48641497-48641516 Msa0603840:intron 20.0%
!! TCACTTAAATTAAATTTGGT+TGG + chr4_2:48641501-48641520 Msa0603840:intron 20.0%
!! AAGCTTAATTATTGGTAAAT+AGG + chr4_2:48641620-48641639 Msa0603840:intron 20.0%
!!! TTTTAATCATGATAAAGTTG+TGG + chr4_2:48641735-48641754 Msa0603840:intron 20.0%
!!! TCAAATACTTTTTTACATGT+AGG + chr4_2:48642051-48642070 Msa0603840:intron 20.0%
!! TTATGTTTGCTTAAAATTGA+TGG + chr4_2:48642755-48642774 Msa0603840:intron 20.0%
!! AATTTCCAGTAAAAATGAAT+AGG + chr4_2:48642823-48642842 Msa0603840:intron 20.0%
!!! TTTCAATTGATTTTCAATTC+AGG + chr4_2:48642892-48642911 Msa0603840:intron 20.0%
!!! GTTCTTTGTTATATTATATG+TGG + chr4_2:48643698-48643717 Msa0603840:three_prime_UTR 20.0%
!!! TTTGTTATATTATATGTGGT+GGG + chr4_2:48643702-48643721 Msa0603840:three_prime_UTR 20.0%
!! ATATAAAAACCTAAACACTA+GGG - chr4_2:48643803-48643822 None:intergenic 20.0%
!! TATATAAAAACCTAAACACT+AGG - chr4_2:48643804-48643823 None:intergenic 20.0%
!!! TTAGGTTTTTATATATGTAC+TGG + chr4_2:48643809-48643828 Msa0603840:three_prime_UTR 20.0%
!!! TTTTTATATATGTACTGGTT+TGG + chr4_2:48643814-48643833 Msa0603840:three_prime_UTR 20.0%
!!! ATTGGCTTTTTATATTAATG+TGG + chr4_2:48643992-48644011 Msa0603840:three_prime_UTR 20.0%
!! TAATAGCTGTTGAATGTTTT+TGG + chr4_2:48641431-48641450 Msa0603840:intron 25.0%
!!! ATTTGGTTGGTAAATTATGT+CGG + chr4_2:48641514-48641533 Msa0603840:intron 25.0%
!! ATATGATTTGTATTAAGCTG+AGG - chr4_2:48641582-48641601 None:intergenic 25.0%
!!! TTGTTCTGAAGCTTAATTAT+TGG + chr4_2:48641612-48641631 Msa0603840:intron 25.0%
! GACTCATTTCAATAAGTAAT+TGG - chr4_2:48641822-48641841 None:intergenic 25.0%
!! TTCAACATTTTCTTTGTAGA+TGG + chr4_2:48642007-48642026 Msa0603840:intron 25.0%
!! TCAACATTTTCTTTGTAGAT+GGG + chr4_2:48642008-48642027 Msa0603840:intron 25.0%
!!! ATACTTTTTTACATGTAGGT+AGG + chr4_2:48642055-48642074 Msa0603840:intron 25.0%
! CATAACTAATATCACCAATT+TGG + chr4_2:48642102-48642121 Msa0603840:intron 25.0%
! TTGAATATAAAGAGCCAAAT+TGG - chr4_2:48642119-48642138 None:intergenic 25.0%
! TCATTCTGCAATATCTTATT+GGG + chr4_2:48642275-48642294 Msa0603840:intron 25.0%
!! ATTTGTATTTCTGTGATAAC+TGG + chr4_2:48642370-48642389 Msa0603840:intron 25.0%
!! TTTGTATTTCTGTGATAACT+GGG + chr4_2:48642371-48642390 Msa0603840:intron 25.0%
! AAGCAAACATAAGAATACAA+TGG - chr4_2:48642747-48642766 None:intergenic 25.0%
!! TTTGCTTAAAATTGATGGTT+TGG + chr4_2:48642760-48642779 Msa0603840:intron 25.0%
!!! TTAAAATTGATGGTTTGGTT+GGG + chr4_2:48642765-48642784 Msa0603840:intron 25.0%
! AGTAAAAATGAATAGGAGTT+GGG + chr4_2:48642830-48642849 Msa0603840:intron 25.0%
! AAAATGACATGAATAAGAGA+TGG - chr4_2:48642961-48642980 None:intergenic 25.0%
!!! ATCTCTAAGCCTTTTTTATT+TGG + chr4_2:48643351-48643370 Msa0603840:CDS 25.0%
! AATTGAGCATAACATATCTA+AGG - chr4_2:48643637-48643656 None:intergenic 25.0%
!! CTTTGTTATATTATATGTGG+TGG + chr4_2:48643701-48643720 Msa0603840:three_prime_UTR 25.0%
! TTGTTATATTATATGTGGTG+GGG + chr4_2:48643703-48643722 Msa0603840:three_prime_UTR 25.0%
! TGCTTAGATTGTATTTATTG+TGG + chr4_2:48643930-48643949 Msa0603840:three_prime_UTR 25.0%
! GCTTAGATTGTATTTATTGT+GGG + chr4_2:48643931-48643950 Msa0603840:three_prime_UTR 25.0%
!! ATTTATTGTGGGTTTTCTAA+TGG + chr4_2:48643942-48643961 Msa0603840:three_prime_UTR 25.0%
!! GATTATAGTTTGTAATCGAT+TGG + chr4_2:48643974-48643993 Msa0603840:three_prime_UTR 25.0%
!! TTTGGTTGGTAAATTATGTC+GGG + chr4_2:48641515-48641534 Msa0603840:intron 30.0%
TAACTGTGTATTACTAGAGA+AGG + chr4_2:48641765-48641784 Msa0603840:intron 30.0%
!!! CTGTTATTTTGATGCAATTC+TGG + chr4_2:48641919-48641938 Msa0603840:intron 30.0%
AATCTCTTAACCAAAAGAGA+AGG + chr4_2:48641958-48641977 Msa0603840:intron 30.0%
ATCTCTTAACCAAAAGAGAA+GGG + chr4_2:48641959-48641978 Msa0603840:intron 30.0%
! CAACATTTTCTTTGTAGATG+GGG + chr4_2:48642009-48642028 Msa0603840:intron 30.0%
GTCATTCTGCAATATCTTAT+TGG + chr4_2:48642274-48642293 Msa0603840:intron 30.0%
TGGGTGCTTAATTGAAAATA+AGG + chr4_2:48642294-48642313 Msa0603840:intron 30.0%
! ATTGAACATCATGGGAATTT+TGG - chr4_2:48642510-48642529 None:intergenic 30.0%
TTAGTCATAACTAAGACACT+GGG - chr4_2:48642685-48642704 None:intergenic 30.0%
ATTAGTCATAACTAAGACAC+TGG - chr4_2:48642686-48642705 None:intergenic 30.0%
!! CTTAAAATTGATGGTTTGGT+TGG + chr4_2:48642764-48642783 Msa0603840:intron 30.0%
!! CAACTCCTATTCATTTTTAC+TGG - chr4_2:48642831-48642850 None:intergenic 30.0%
CAGTAAAAATGAATAGGAGT+TGG + chr4_2:48642829-48642848 Msa0603840:intron 30.0%
TAATTTAATCTTTGGGTCCT+TGG + chr4_2:48643004-48643023 Msa0603840:intron 30.0%
! ATCAACAACTGTGGATTTTT+CGG + chr4_2:48643099-48643118 Msa0603840:CDS 30.0%
TCCCTTGGAAATATTATGAA+TGG + chr4_2:48643204-48643223 Msa0603840:CDS 30.0%
CCCATTCATAATATTTCCAA+GGG - chr4_2:48643208-48643227 None:intergenic 30.0%
CCCTTGGAAATATTATGAAT+GGG + chr4_2:48643205-48643224 Msa0603840:CDS 30.0%
ACCCATTCATAATATTTCCA+AGG - chr4_2:48643209-48643228 None:intergenic 30.0%
! ATCTAAGGTTGTAAAACTTC+TGG - chr4_2:48643622-48643641 None:intergenic 30.0%
ACAAAGAACACCAATAAGAT+GGG - chr4_2:48643687-48643706 None:intergenic 30.0%
AACAAAGAACACCAATAAGA+TGG - chr4_2:48643688-48643707 None:intergenic 30.0%
TGTTATATTATATGTGGTGG+GGG + chr4_2:48643704-48643723 Msa0603840:three_prime_UTR 30.0%
ATATGGAAATATCTGCAGTT+TGG + chr4_2:48643742-48643761 Msa0603840:three_prime_UTR 30.0%
TATGGAAATATCTGCAGTTT+GGG + chr4_2:48643743-48643762 Msa0603840:three_prime_UTR 30.0%
!!! GTTTGGGACTTTATTTTGAT+TGG + chr4_2:48643759-48643778 Msa0603840:three_prime_UTR 30.0%
!! TTATATATGTACTGGTTTGG+TGG + chr4_2:48643817-48643836 Msa0603840:three_prime_UTR 30.0%
AATACAATCTAAGCATATGC+TGG - chr4_2:48643925-48643944 None:intergenic 30.0%
AAAAAAAGAAGAATTGGAGC+TGG + chr4_2:48644034-48644053 Msa0603840:three_prime_UTR 30.0%
GATTTCGTTGAAATTTGCAG+TGG + chr4_2:48641374-48641393 Msa0603840:intron 35.0%
!!! GTTTGGTGTTATTGCTTTTG+CGG + chr4_2:48641408-48641427 Msa0603840:intron 35.0%
!!! TAGCTGTTGAATGTTTTTGG+TGG + chr4_2:48641434-48641453 Msa0603840:intron 35.0%
!!! GAATGTTTTTGGTGGTTTGT+TGG + chr4_2:48641442-48641461 Msa0603840:intron 35.0%
! ATGTGGTTCTATTGCATGTT+TGG + chr4_2:48641465-48641484 Msa0603840:intron 35.0%
! TTCTAAAAACAGAGAGAGCA+TGG + chr4_2:48641876-48641895 Msa0603840:intron 35.0%
! CTATCATTACCCTTCTCTTT+TGG - chr4_2:48641971-48641990 None:intergenic 35.0%
! TTTTCTTTGTAGATGGGGAT+AGG + chr4_2:48642014-48642033 Msa0603840:intron 35.0%
TTGTGTGTTTGATAAGAAGC+AGG + chr4_2:48642147-48642166 Msa0603840:intron 35.0%
TGTGTGTTTGATAAGAAGCA+GGG + chr4_2:48642148-48642167 Msa0603840:intron 35.0%
AGAGGACATGCATTTAAACA+AGG - chr4_2:48642192-48642211 None:intergenic 35.0%
CGAAAAGACTATAAGTTGCA+TGG - chr4_2:48642255-48642274 None:intergenic 35.0%
CAATTAAGCACGGAAATACT+GGG - chr4_2:48642326-48642345 None:intergenic 35.0%
ACAATTAAGCACGGAAATAC+TGG - chr4_2:48642327-48642346 None:intergenic 35.0%
GACAATCACACAATTAAGCA+CGG - chr4_2:48642336-48642355 None:intergenic 35.0%
GGTGATAGATTGAATATCAC+AGG - chr4_2:48642455-48642474 None:intergenic 35.0%
TGAGACTTACACAAGAAAGA+GGG - chr4_2:48642476-48642495 None:intergenic 35.0%
TTGAGACTTACACAAGAAAG+AGG - chr4_2:48642477-48642496 None:intergenic 35.0%
GGTGATAGATTGAACATCAT+GGG - chr4_2:48642518-48642537 None:intergenic 35.0%
TGGGATTTACAAAAGACACA+GGG - chr4_2:48642539-48642558 None:intergenic 35.0%
TTGGGATTTACAAAAGACAC+AGG - chr4_2:48642540-48642559 None:intergenic 35.0%
! TGGATTCTGTTTTCTCTTTG+TGG + chr4_2:48642647-48642666 Msa0603840:intron 35.0%
TAGTCATAACTAAGACACTG+GGG - chr4_2:48642684-48642703 None:intergenic 35.0%
TGACATGAATAAGAGATGGA+AGG - chr4_2:48642957-48642976 None:intergenic 35.0%
! GTGCAGGAAATTTGAAACAA+TGG + chr4_2:48643026-48643045 Msa0603840:intron 35.0%
CAGTTGTTGATGCACAATAT+AGG - chr4_2:48643090-48643109 None:intergenic 35.0%
ATATTGTGCATCAACAACTG+TGG + chr4_2:48643090-48643109 Msa0603840:CDS 35.0%
AAAGGCTTAGAGATAGTTTC+AGG - chr4_2:48643345-48643364 None:intergenic 35.0%
!!! AAGCCTTTTTTATTTGGCTC+AGG + chr4_2:48643357-48643376 Msa0603840:CDS 35.0%
GAACCTGAGCCAAATAAAAA+AGG - chr4_2:48643363-48643382 None:intergenic 35.0%
GCTTGATAAGATCTGAGAAT+TGG + chr4_2:48643593-48643612 Msa0603840:exon 35.0%
TTGCACATACCAATCTGAAA+CGG - chr4_2:48643874-48643893 None:intergenic 35.0%
AAGAGAAAAAACGACCACAC+GGG - chr4_2:48641315-48641334 None:intergenic 40.0%
GAAGAGAAAAAACGACCACA+CGG - chr4_2:48641316-48641335 None:intergenic 40.0%
AATTCAACAACACGAGACCA+CGG - chr4_2:48641348-48641367 None:intergenic 40.0%
!!! TTTTGGTGGTTTGTTGGATG+TGG + chr4_2:48641448-48641467 Msa0603840:intron 40.0%
TGCATGTTTGGTATCACAGT+AGG + chr4_2:48641477-48641496 Msa0603840:intron 40.0%
GTAGCTTACGCAAAATTACG+TGG - chr4_2:48641541-48641560 None:intergenic 40.0%
TAAGCTACACACCGTGATTT+CGG + chr4_2:48641553-48641572 Msa0603840:intron 40.0%
! TTCACTCGAGGTATCAAACA+AGG - chr4_2:48641793-48641812 None:intergenic 40.0%
AATTGGCACATATTCACTCG+AGG - chr4_2:48641805-48641824 None:intergenic 40.0%
! AAACAGAGAGAGCATGGAAA+AGG + chr4_2:48641882-48641901 Msa0603840:intron 40.0%
AGAGCATGGAAAAGGTCTAT+TGG + chr4_2:48641890-48641909 Msa0603840:intron 40.0%
TTGACTACCATGCTTATGAG+AGG - chr4_2:48642210-48642229 None:intergenic 40.0%
ATTTCTGTGATAACTGGGTC+AGG + chr4_2:48642376-48642395 Msa0603840:intron 40.0%
! AATCTCTGCTGCTTGGTAAT+GGG - chr4_2:48642432-48642451 None:intergenic 40.0%
TTCTTGTGTAAGTCTCAACC+TGG + chr4_2:48642479-48642498 Msa0603840:intron 40.0%
GGGTGATAGATTGAACATCA+TGG - chr4_2:48642519-48642538 None:intergenic 40.0%
! AATTTTGCCTGCTGCAGATT+GGG - chr4_2:48642558-48642577 None:intergenic 40.0%
! AAATTTTGCCTGCTGCAGAT+TGG - chr4_2:48642559-48642578 None:intergenic 40.0%
! AAAGTAGTGGAGACTTCCAA+TGG - chr4_2:48642595-48642614 None:intergenic 40.0%
ACAGATGGAAAGCAAAGTAG+TGG - chr4_2:48642608-48642627 None:intergenic 40.0%
! TGCTTTCCATCTGTCATTTC+CGG + chr4_2:48642614-48642633 Msa0603840:intron 40.0%
GAGAAAACAGAATCCATGTC+TGG - chr4_2:48642643-48642662 None:intergenic 40.0%
AAGACACTGGGGAAGATAAA+GGG - chr4_2:48642673-48642692 None:intergenic 40.0%
TAAGACACTGGGGAAGATAA+AGG - chr4_2:48642674-48642693 None:intergenic 40.0%
!! TGGGTTTGTTAGTTTGAGCT+TGG + chr4_2:48642784-48642803 Msa0603840:intron 40.0%
!! GGGTTTGTTAGTTTGAGCTT+GGG + chr4_2:48642785-48642804 Msa0603840:intron 40.0%
TGAATAAGAGATGGAAGGAG+AGG - chr4_2:48642952-48642971 None:intergenic 40.0%
GTTTCAAATTTCCTGCACCA+AGG - chr4_2:48643024-48643043 None:intergenic 40.0%
TGCACAATATAGGACCTTCA+GGG - chr4_2:48643080-48643099 None:intergenic 40.0%
ATGCACAATATAGGACCTTC+AGG - chr4_2:48643081-48643100 None:intergenic 40.0%
GTTTCAGCATCATGTCTTTG+TGG - chr4_2:48643158-48643177 None:intergenic 40.0%
CAAAGACATGATGCTGAAAC+AGG + chr4_2:48643158-48643177 Msa0603840:CDS 40.0%
!!! AGTTTTAGGTTCCACGGAAA+TGG - chr4_2:48643289-48643308 None:intergenic 40.0%
! ATATCGACACTGGCAGTTTT+AGG - chr4_2:48643303-48643322 None:intergenic 40.0%
!! AGTTTCAGGTTCCACAGAAA+TGG - chr4_2:48643331-48643350 None:intergenic 40.0%
! TTTATTTGGCTCAGGTTCAG+AGG + chr4_2:48643365-48643384 Msa0603840:CDS 40.0%
!! AATAAGCGAGTTGGTTTGAC+AGG + chr4_2:48643438-48643457 Msa0603840:CDS 40.0%
!! ATAAGCGAGTTGGTTTGACA+GGG + chr4_2:48643439-48643458 Msa0603840:CDS 40.0%
ACAGGGTTTAATTGTCGATG+TGG + chr4_2:48643456-48643475 Msa0603840:CDS 40.0%
ATAAGATGGGCAGGAAACTT+GGG - chr4_2:48643674-48643693 None:intergenic 40.0%
AATAAGATGGGCAGGAAACT+TGG - chr4_2:48643675-48643694 None:intergenic 40.0%
AAGTTTCCTGCCCATCTTAT+TGG + chr4_2:48643674-48643693 Msa0603840:three_prime_UTR 40.0%
GTATCTTTGCCCTAGTGTTT+AGG + chr4_2:48643791-48643810 Msa0603840:three_prime_UTR 40.0%
ACGGGAAAGATGCATTACAA+AGG - chr4_2:48643855-48643874 None:intergenic 40.0%
! TGCACATACCAATCTGAAAC+GGG - chr4_2:48643873-48643892 None:intergenic 40.0%
CAGTGGTGAGCTCAATTGTT+TGG + chr4_2:48641391-48641410 Msa0603840:intron 45.0%
AGCTGAGGTTTCCGAAATCA+CGG - chr4_2:48641567-48641586 None:intergenic 45.0%
TGAGTCATCATGCATGCACT+TGG + chr4_2:48641836-48641855 Msa0603840:intron 45.0%
TGTAGATGGGGATAGGATAG+TGG + chr4_2:48642021-48642040 Msa0603840:intron 45.0%
TGCATGTCCTCTCATAAGCA+TGG + chr4_2:48642200-48642219 Msa0603840:intron 45.0%
!! ATCTCTGCTGCTTGGTAATG+GGG - chr4_2:48642431-48642450 None:intergenic 45.0%
! GAATCTCTGCTGCTTGGTAA+TGG - chr4_2:48642433-48642452 None:intergenic 45.0%
TGTAAATCCCAATCTGCAGC+AGG + chr4_2:48642548-48642567 Msa0603840:intron 45.0%
TTTGTTCTCAGCTGCACCAT+TGG + chr4_2:48642576-48642595 Msa0603840:intron 45.0%
TAAGAGATGGAAGGAGAGGA+AGG - chr4_2:48642948-48642967 None:intergenic 45.0%
CACAATATAGGACCTTCAGG+GGG - chr4_2:48643078-48643097 None:intergenic 45.0%
GCACAATATAGGACCTTCAG+GGG - chr4_2:48643079-48643098 None:intergenic 45.0%
GCACTTAGAGCACATGTTCA+TGG - chr4_2:48643136-48643155 None:intergenic 45.0%
AAGGGATGATGCTGCAAGAT+GGG - chr4_2:48643190-48643209 None:intergenic 45.0%
!!! ACTGGCAGTTTTAGGTTCCA+CGG - chr4_2:48643295-48643314 None:intergenic 45.0%
AATGGATGGGATATCGACAC+TGG - chr4_2:48643313-48643332 None:intergenic 45.0%
CGATATCCCATCCATTTCTG+TGG + chr4_2:48643317-48643336 Msa0603840:CDS 45.0%
CAGGTTCCACAGAAATGGAT+GGG - chr4_2:48643326-48643345 None:intergenic 45.0%
TCAGGTTCCACAGAAATGGA+TGG - chr4_2:48643327-48643346 None:intergenic 45.0%
TTGCAGTTGCTGCAACGTTT+CGG - chr4_2:48643420-48643439 None:intergenic 45.0%
AGCAACTGCAATAAGCGAGT+TGG + chr4_2:48643429-48643448 Msa0603840:CDS 45.0%
AGCGATGTACAGCACAGAAA+AGG - chr4_2:48643485-48643504 None:intergenic 45.0%
AGAACACCAATAAGATGGGC+AGG - chr4_2:48643683-48643702 None:intergenic 45.0%
!! TTATATGTGGTGGGGGTACT+TGG + chr4_2:48643711-48643730 Msa0603840:three_prime_UTR 45.0%
!! GGTACTTGGTCTTGGTCATA+TGG + chr4_2:48643725-48643744 Msa0603840:three_prime_UTR 45.0%
!! TGTACTGGTTTGGTGGAAGT+TGG + chr4_2:48643824-48643843 Msa0603840:three_prime_UTR 45.0%
GCATCTTTCCCGTTTCAGAT+TGG + chr4_2:48643862-48643881 Msa0603840:three_prime_UTR 45.0%
TTCTCTTCTTCTCGAAGCCG+TGG + chr4_2:48641328-48641347 Msa0603840:intron 50.0%
!! CTTGGTAATGGGGTTGCAAG+AGG - chr4_2:48642421-48642440 None:intergenic 50.0%
TCACAGGAATCTCTGCTGCT+TGG - chr4_2:48642439-48642458 None:intergenic 50.0%
! AATCTTTGGGTCCTTGGTGC+AGG + chr4_2:48643010-48643029 Msa0603840:intron 50.0%
CATGATGCTGAAACAGGAGC+AGG + chr4_2:48643164-48643183 Msa0603840:CDS 50.0%
CAAGGGATGATGCTGCAAGA+TGG - chr4_2:48643191-48643210 None:intergenic 50.0%
CATCTTGCAGCATCATCCCT+TGG + chr4_2:48643189-48643208 Msa0603840:CDS 50.0%
TAGGTTCCACGGAAATGGCT+GGG - chr4_2:48643284-48643303 None:intergenic 50.0%
TTAGGTTCCACGGAAATGGC+TGG - chr4_2:48643285-48643304 None:intergenic 50.0%
CAACGTTTCGGACCATCCTT+CGG - chr4_2:48643408-48643427 None:intergenic 50.0%
! CAGAAGTGCGGTAATCGAAC+GGG - chr4_2:48643527-48643546 None:intergenic 50.0%
! GCAGAAGTGCGGTAATCGAA+CGG - chr4_2:48643528-48643547 None:intergenic 50.0%
TGCCATAGCCAAAGCAAACC+CGG + chr4_2:48643554-48643573 Msa0603840:CDS 50.0%
! AACCGGGTTTGCTTTGGCTA+TGG - chr4_2:48643559-48643578 None:intergenic 50.0%
! CTTGACAACCGGGTTTGCTT+TGG - chr4_2:48643565-48643584 None:intergenic 50.0%
CAAAGCAAACCCGGTTGTCA+AGG + chr4_2:48643563-48643582 Msa0603840:CDS 50.0%
AGCTTCTCAGCCTTGACAAC+CGG - chr4_2:48643576-48643595 None:intergenic 50.0%
GAATAGAGTTGCAGCCCGTG+TGG + chr4_2:48641298-48641317 Msa0603840:intron 55.0%
ATGGGGTTGCAAGAGGCTGA+TGG - chr4_2:48642414-48642433 None:intergenic 55.0%
!!! ATGGGAATTTTGGCTGCGCC+AGG - chr4_2:48642500-48642519 None:intergenic 55.0%
TGGCAGCCGGAAATGACAGA+TGG - chr4_2:48642623-48642642 None:intergenic 55.0%
TCATTTCCGGCTGCCAGACA+TGG + chr4_2:48642627-48642646 Msa0603840:intron 55.0%
CAGAATCCATGTCTGGCAGC+CGG - chr4_2:48642636-48642655 None:intergenic 55.0%
GAATGTCAAGCTCCCCCTGA+AGG + chr4_2:48643063-48643082 Msa0603840:CDS 55.0%
ACGCTGGCAGCAGTAACAAC+AGG - chr4_2:48643255-48643274 None:intergenic 55.0%
TGTTGTTACTGCTGCCAGCG+TGG + chr4_2:48643254-48643273 Msa0603840:CDS 55.0%
AATGGCTGGGATATCCACGC+TGG - chr4_2:48643271-48643290 None:intergenic 55.0%
GGATATCCCAGCCATTTCCG+TGG + chr4_2:48643275-48643294 Msa0603840:CDS 55.0%
GAGTGGTGATCCAAAGCCGA+AGG + chr4_2:48643389-48643408 Msa0603840:CDS 55.0%
GGTGATCCAAAGCCGAAGGA+TGG + chr4_2:48643393-48643412 Msa0603840:CDS 55.0%
TTCGGACCATCCTTCGGCTT+TGG - chr4_2:48643402-48643421 None:intergenic 55.0%
TGGCATCTCGTGCAGAAGTG+CGG - chr4_2:48643539-48643558 None:intergenic 55.0%
GCTTCTCAGCCTTGACAACC+GGG - chr4_2:48643575-48643594 None:intergenic 55.0%
GGCTCAGGTTCAGAGGAGAG+TGG + chr4_2:48643372-48643391 Msa0603840:CDS 60.0%
!! GTGGTGGGGGTACTTGGTCT+TGG + chr4_2:48643717-48643736 Msa0603840:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 48641279 48644068 48641279 ID=Msa0603840;Name=Msa0603840
chr4_2 mRNA 48641279 48644068 48641279 ID=Msa0603840-mRNA-1;Parent=Msa0603840;Name=Msa0603840-mRNA-1;_AED=0.13;_eAED=0.14;_QI=0|0|0.5|1|0|0.5|2|460|194
chr4_2 exon 48641279 48641286 48641279 ID=Msa0603840-mRNA-1:exon:11230;Parent=Msa0603840-mRNA-1
chr4_2 exon 48643032 48644068 48643032 ID=Msa0603840-mRNA-1:exon:11231;Parent=Msa0603840-mRNA-1
chr4_2 CDS 48641279 48641286 48641279 ID=Msa0603840-mRNA-1:cds;Parent=Msa0603840-mRNA-1
chr4_2 CDS 48643032 48643608 48643032 ID=Msa0603840-mRNA-1:cds;Parent=Msa0603840-mRNA-1
chr4_2 three_prime_UTR 48643609 48644068 48643609 ID=Msa0603840-mRNA-1:three_prime_utr;Parent=Msa0603840-mRNA-1
Gene Sequence

>Msa0603840

GTATTCAGGAAATTTGAAACAATGGAACATGAAAAGACTGAATGTCAAGCTCCCCCTGAAGGTCCTATATTGTGCATCAACAACTGTGGATTTTTCGGAAGTGCAGCTACCATGAACATGTGCTCTAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCCCATCTTGCAGCATCATCCCTTGGAAATATTATGAATGGGTCTACAAGCAACACTGAAAAAGAACCTGTTGTTACTGCTGCCAGCGTGGATATCCCAGCCATTTCCGTGGAACCTAAAACTGCCAGTGTCGATATCCCATCCATTTCTGTGGAACCTGAAACTATCTCTAAGCCTTTTTTATTTGGCTCAGGTTCAGAGGAGAGTGGTGATCCAAAGCCGAAGGATGGTCCGAAACGTTGCAGCAACTGCAATAAGCGAGTTGGTTTGACAGGGTTTAATTGTCGATGTGGCAACCTTTTCTGTGCTGTACATCGCTACTCAGACAAGCATGATTGCCCGTTCGATTACCGCACTTCTGCACGAGATGCCATAGCCAAAGCAAACCCGGTTGTCAAGGCTGAGAAGCTTGATAAGATCTGA
Protein sequence

>Msa0603840

VFRKFETMEHEKTECQAPPEGPILCINNCGFFGSAATMNMCSKCHKDMMLKQEQAHLAASSLGNIMNGSTSNTEKEPVVTAASVDIPAISVEPKTASVDIPSISVEPETISKPFLFGSGSEESGDPKPKDGPKRCSNCNKRVGLTGFNCRCGNLFCAVHRYSDKHDCPFDYRTSARDAIAKANPVVKAEKLDKI*