AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1193950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1193950 MtrunA17_Chr8g0364791 84.252 381 43 3 1 380 80 444 0.0 634
Msa1193950 MtrunA17_Chr8g0384201 68.949 409 87 11 1 374 80 483 0.0 515
Msa1193950 MtrunA17_Chr3g0130101 61.935 155 54 2 1 150 81 235 1.36e-61 202
Msa1193950 MtrunA17_Chr1g0197361 57.292 96 40 1 2 97 84 178 3.12e-36 139
Msa1193950 MtrunA17_Chr1g0201021 56.436 101 44 0 2 102 94 194 2.50e-34 133
Msa1193950 MtrunA17_Chr3g0092671 52.041 98 46 1 2 99 81 177 1.62e-33 130
Msa1193950 MtrunA17_Chr1g0197351 43.421 152 70 6 1 146 116 257 9.63e-27 110
Msa1193950 MtrunA17_Chr6g0481091 56.410 78 34 0 2 79 81 158 1.38e-26 106
Msa1193950 MtrunA17_Chr2g0309331 46.875 96 44 3 2 96 86 175 1.14e-23 100
Msa1193950 MtrunA17_Chr6g0484441 52.632 76 34 1 2 77 78 151 2.62e-23 99.4
Msa1193950 MtrunA17_Chr4g0060931 48.810 84 41 1 2 83 90 173 2.79e-23 99.8
Msa1193950 MtrunA17_Chr3g0113031 42.857 98 48 4 2 93 13 108 2.97e-23 97.8
Msa1193950 MtrunA17_Chr5g0433781 48.315 89 45 1 2 89 81 169 3.55e-23 101
Msa1193950 MtrunA17_Chr4g0056541 44.762 105 50 4 2 104 83 181 8.52e-23 98.6
Msa1193950 MtrunA17_Chr3g0109821 48.315 89 44 2 2 88 81 169 9.53e-23 100
Msa1193950 MtrunA17_Chr7g0217721 40.000 105 47 2 2 90 92 196 9.56e-23 98.2
Msa1193950 MtrunA17_Chr3g0091921 47.619 84 42 1 2 83 89 172 1.37e-22 96.7
Msa1193950 MtrunA17_Chr3g0139331 47.619 84 40 2 2 83 84 165 3.31e-22 96.3
Msa1193950 MtrunA17_Chr6g0453451 42.708 96 49 2 2 93 93 186 3.81e-22 95.9
Msa1193950 MtrunA17_Chr1g0200561 48.276 87 41 2 2 87 95 178 9.32e-22 95.1
Msa1193950 MtrunA17_Chr5g0428991 48.718 78 40 0 1 78 99 176 1.31e-21 96.7
Msa1193950 MtrunA17_Chr4g0017921 51.282 78 33 2 2 78 84 157 1.81e-21 94.4
Msa1193950 MtrunA17_Chr3g0128151 50.649 77 38 0 1 77 96 172 1.93e-21 96.3
Msa1193950 MtrunA17_Chr8g0346001 43.023 86 40 1 2 78 91 176 1.97e-21 95.1
Msa1193950 MtrunA17_Chr2g0316971 50.588 85 36 3 2 81 96 179 3.84e-21 94.0
Msa1193950 MtrunA17_Chr2g0309891 53.247 77 35 1 2 77 81 157 4.65e-21 93.2
Msa1193950 MtrunA17_Chr3g0128111 50.633 79 35 2 1 77 116 192 6.28e-21 90.9
Msa1193950 MtrunA17_Chr3g0113001 40.206 97 51 2 2 93 38 132 2.01e-20 90.5
Msa1193950 MtrunA17_Chr5g0398971 41.818 110 53 4 2 108 83 184 2.07e-20 91.3
Msa1193950 MtrunA17_Chr4g0018391 40.000 105 54 4 2 104 83 180 2.19e-20 91.3
Msa1193950 MtrunA17_Chr8g0372121 48.052 77 38 1 2 78 82 156 2.44e-20 90.5
Msa1193950 MtrunA17_Chr6g0461661 50.617 81 34 3 2 77 91 170 3.25e-20 91.3
Msa1193950 MtrunA17_Chr2g0312321 48.052 77 39 1 2 77 82 158 7.26e-20 89.0
Msa1193950 MtrunA17_Chr7g0253011 47.561 82 37 2 2 77 90 171 1.62e-19 89.4
Msa1193950 MtrunA17_Chr7g0214071 42.045 88 47 2 2 87 85 170 1.96e-19 87.0
Msa1193950 MtrunA17_Chr1g0147421 47.436 78 37 2 2 77 101 176 2.87e-19 88.2
Msa1193950 MtrunA17_Chr7g0217711 50.794 63 31 0 2 64 92 154 3.00e-19 84.3
Msa1193950 MtrunA17_Chr5g0405211 46.753 77 39 1 2 78 84 158 3.30e-19 87.8
Msa1193950 MtrunA17_Chr2g0318081 43.421 76 43 0 2 77 89 164 1.30e-18 86.3
Msa1193950 MtrunA17_Chr4g0054791 43.529 85 45 1 2 83 81 165 6.00e-18 84.0
Msa1193950 MtrunA17_Chr3g0124761 44.444 81 41 2 2 79 80 159 1.09e-17 82.4
Msa1193950 MtrunA17_Chr4g0042561 40.476 84 46 2 2 81 82 165 1.63e-17 81.6
Msa1193950 MtrunA17_Chr4g0038751 49.367 79 32 2 2 77 88 161 2.07e-17 82.0
Msa1193950 MtrunA17_Chr1g0183741 32.121 165 92 5 2 162 83 231 2.83e-17 81.3
Msa1193950 MtrunA17_Chr2g0321741 43.836 73 34 1 2 74 66 131 2.96e-17 82.4
Msa1193950 MtrunA17_Chr8g0385991 39.583 96 47 3 1 89 84 175 5.40e-17 79.3
Msa1193950 MtrunA17_Chr5g0400461 40.000 105 53 2 2 99 81 182 1.15e-16 80.5
Msa1193950 MtrunA17_Chr7g0252971 31.618 136 68 5 2 122 81 206 2.26e-16 79.3
Msa1193950 MtrunA17_Chr8g0384721 40.244 82 44 2 2 79 80 160 3.09e-16 78.2
Msa1193950 MtrunA17_Chr7g0261411 45.455 77 36 2 2 76 83 155 4.15e-16 78.2
Msa1193950 MtrunA17_Chr8g0359921 41.026 78 44 1 2 77 87 164 4.31e-16 78.6
Msa1193950 MtrunA17_Chr5g0417791 39.506 81 45 2 2 78 76 156 4.69e-16 77.4
Msa1193950 MtrunA17_Chr3g0144271 52.459 61 27 1 2 60 97 157 6.30e-16 79.0
Msa1193950 MtrunA17_Chr4g0076731 44.304 79 41 3 2 79 127 203 6.81e-16 79.0
Msa1193950 MtrunA17_Chr8g0361731 40.230 87 41 3 2 77 87 173 6.84e-16 78.6
Msa1193950 MtrunA17_Chr2g0318101 35.652 115 56 3 2 103 88 197 1.91e-15 77.0
Msa1193950 MtrunA17_Chr3g0131911 41.053 95 46 2 1 85 84 178 2.28e-15 75.1
Msa1193950 MtrunA17_Chr6g0453131 41.860 86 40 4 2 77 88 173 3.15e-15 76.6
Msa1193950 MtrunA17_Chr3g0130731 39.506 81 45 2 2 79 80 159 5.00e-15 75.1
Msa1193950 MtrunA17_Chr7g0263931 36.957 92 51 3 2 87 84 174 7.81e-15 74.7
Msa1193950 MtrunA17_Chr7g0243371 51.852 54 24 1 3 54 38 91 3.15e-14 68.6
Msa1193950 MtrunA17_Chr5g0417001 38.372 86 43 3 2 77 87 172 3.62e-14 73.2
Msa1193950 MtrunA17_Chr2g0311651 37.363 91 42 2 2 77 96 186 5.82e-14 72.0
Msa1193950 MtrunA17_Chr2g0326361 39.024 82 44 3 2 77 87 168 3.90e-13 69.7
Msa1193950 MtrunA17_Chr2g0321631 39.024 82 46 2 2 79 83 164 5.56e-13 68.2
Msa1193950 MtrunA17_Chr2g0311661 52.632 57 23 1 2 54 95 151 1.06e-12 68.2
Msa1193950 MtrunA17_Chr5g0442771 35.227 88 45 3 2 77 91 178 1.83e-12 67.4
Msa1193950 MtrunA17_Chr4g0047841 50.909 55 25 1 2 54 88 142 2.65e-12 67.4
Msa1193950 MtrunA17_Chr7g0266701 32.927 82 49 3 2 77 83 164 4.65e-12 65.9
Msa1193950 MtrunA17_Chr8g0345691 42.105 76 36 4 1 74 87 156 5.04e-12 67.0
Msa1193950 MtrunA17_Chr4g0050701 36.471 85 46 2 2 78 76 160 5.48e-11 61.2
Msa1193950 MtrunA17_Chr4g0026141 35.366 82 49 2 2 79 83 164 8.57e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1193950 AT5G09330.5 48.000 175 77 3 2 164 81 253 3.31e-46 165
Msa1193950 AT5G09330.2 48.000 175 77 3 2 164 81 253 3.31e-46 165
Msa1193950 AT5G09330.4 48.000 175 77 3 2 164 81 253 3.31e-46 165
Msa1193950 AT5G09330.3 48.000 175 77 3 2 164 81 253 3.31e-46 165
Msa1193950 AT5G09330.1 48.000 175 77 3 2 164 81 253 3.31e-46 165
Msa1193950 AT5G64060.1 50.000 152 66 2 2 149 81 226 5.81e-43 153
Msa1193950 AT5G04410.1 58.586 99 41 0 2 100 84 182 2.35e-40 150
Msa1193950 AT3G10500.1 60.440 91 36 0 2 92 84 174 9.41e-37 140
Msa1193950 AT3G10490.1 54.902 102 44 2 1 101 101 201 7.65e-34 126
Msa1193950 AT3G10490.4 54.902 102 44 2 1 101 1 101 1.52e-33 128
Msa1193950 AT3G10490.3 54.902 102 44 2 1 101 1 101 1.52e-33 128
Msa1193950 AT1G34190.1 54.000 100 46 0 2 101 91 190 5.76e-33 129
Msa1193950 AT3G10490.2 54.902 102 44 2 1 101 101 201 9.60e-33 127
Msa1193950 AT1G34180.3 48.624 109 50 1 3 105 92 200 4.47e-32 125
Msa1193950 AT1G34180.4 48.624 109 50 1 3 105 104 212 5.24e-32 125
Msa1193950 AT3G10480.1 55.000 100 43 2 1 99 101 199 6.73e-32 125
Msa1193950 AT1G34180.1 48.182 110 51 1 2 105 91 200 8.43e-32 126
Msa1193950 AT3G10480.2 53.535 99 45 1 1 99 101 198 9.14e-32 125
Msa1193950 AT1G34180.2 48.182 110 51 1 2 105 103 212 1.14e-31 126
Msa1193950 AT3G10480.3 55.000 100 43 2 1 99 134 232 1.23e-31 125
Msa1193950 AT1G32870.2 54.348 92 40 1 3 92 84 175 6.47e-28 115
Msa1193950 AT1G32870.1 54.348 92 40 1 3 92 84 175 6.73e-28 115
Msa1193950 AT1G32870.3 54.348 92 40 1 3 92 120 211 1.11e-27 114
Msa1193950 AT3G17730.1 57.692 78 33 0 2 79 81 158 8.21e-27 107
Msa1193950 AT3G12977.2 52.439 82 38 1 2 82 38 119 1.07e-25 103
Msa1193950 AT3G12977.1 53.086 81 37 1 2 81 91 171 9.55e-25 102
Msa1193950 AT1G65910.1 48.276 87 45 0 2 88 81 167 1.08e-24 106
Msa1193950 AT1G33060.2 55.263 76 34 0 2 77 99 174 1.09e-24 106
Msa1193950 AT1G33060.1 55.263 76 34 0 2 77 99 174 1.13e-24 106
Msa1193950 AT3G49530.2 43.802 121 53 3 2 121 88 194 6.66e-24 102
Msa1193950 AT5G61430.2 50.000 84 40 1 2 83 26 109 7.83e-24 100
Msa1193950 AT3G49530.1 43.802 121 53 3 2 121 88 194 7.97e-24 102
Msa1193950 AT4G10350.2 50.649 77 38 0 2 78 38 114 2.75e-23 99.0
Msa1193950 AT5G61430.1 50.000 84 40 1 2 83 89 172 2.92e-23 99.8
Msa1193950 AT5G39610.1 50.000 84 40 1 2 83 93 176 3.75e-23 98.2
Msa1193950 AT4G10350.1 50.649 77 38 0 2 78 85 161 6.67e-23 98.6
Msa1193950 AT1G71930.2 46.341 82 42 1 2 83 85 164 7.18e-23 98.2
Msa1193950 AT1G71930.1 46.341 82 42 1 2 83 85 164 7.18e-23 98.2
Msa1193950 AT4G35580.1 51.316 76 37 0 2 77 84 159 3.24e-22 98.6
Msa1193950 AT4G35580.3 51.316 76 37 0 2 77 84 159 3.27e-22 98.6
Msa1193950 AT1G62700.1 34.694 147 75 6 2 143 83 213 3.30e-22 97.4
Msa1193950 AT1G62700.2 34.694 147 75 6 2 143 83 213 3.30e-22 97.4
Msa1193950 AT4G35580.2 51.316 76 37 0 2 77 84 159 3.31e-22 98.6
Msa1193950 AT1G33280.1 49.351 77 36 1 2 78 84 157 3.34e-22 96.3
Msa1193950 AT1G56010.1 44.792 96 50 1 2 94 26 121 3.38e-22 95.1
Msa1193950 AT1G76420.1 55.000 80 33 2 2 79 95 173 3.40e-22 96.7
Msa1193950 AT1G12260.1 40.367 109 57 3 2 108 83 185 4.89e-22 97.1
Msa1193950 AT1G12260.2 40.367 109 57 3 2 108 83 185 5.38e-22 96.3
Msa1193950 AT5G24590.2 50.633 79 38 1 1 78 87 165 5.67e-22 97.4
Msa1193950 AT2G24430.2 52.564 78 34 2 2 77 89 165 7.92e-22 95.1
Msa1193950 AT2G24430.1 52.564 78 34 2 2 77 89 165 7.92e-22 95.1
Msa1193950 AT1G56010.2 44.792 96 50 1 2 94 93 188 1.06e-21 95.1
Msa1193950 AT5G07680.2 47.059 85 41 2 2 83 76 159 1.49e-21 94.4
Msa1193950 AT5G07680.1 47.059 85 41 2 2 83 90 173 1.83e-21 94.4
Msa1193950 AT3G18400.1 45.122 82 44 1 2 83 78 158 2.55e-21 94.0
Msa1193950 AT1G79580.3 48.718 78 36 2 2 78 93 167 2.67e-21 94.7
Msa1193950 AT1G79580.5 48.718 78 36 2 2 78 93 167 2.67e-21 94.7
Msa1193950 AT1G79580.4 48.718 78 36 2 2 78 93 167 2.67e-21 94.7
Msa1193950 AT1G79580.2 48.718 78 36 2 2 78 93 167 2.67e-21 94.7
Msa1193950 AT1G79580.1 48.718 78 36 2 2 78 93 167 2.67e-21 94.7
Msa1193950 AT5G18270.2 50.000 84 35 3 2 80 94 175 1.08e-20 92.4
Msa1193950 AT5G18270.1 50.000 84 35 3 2 80 94 175 1.19e-20 92.4
Msa1193950 AT2G46770.1 42.222 90 39 2 2 78 92 181 1.78e-20 92.0
Msa1193950 AT3G04060.1 48.780 82 39 2 2 80 93 174 1.85e-20 91.7
Msa1193950 AT3G29035.1 44.565 92 49 1 2 91 97 188 2.99e-20 90.9
Msa1193950 AT5G13180.1 49.412 85 40 2 2 84 84 167 3.09e-20 89.4
Msa1193950 AT4G36160.1 43.434 99 43 3 2 98 86 173 3.15e-20 91.7
Msa1193950 AT4G36160.3 43.434 99 43 3 2 98 86 173 3.15e-20 91.7
Msa1193950 AT2G18060.3 46.341 82 38 2 2 81 85 162 3.30e-20 91.3
Msa1193950 AT2G18060.1 46.341 82 38 2 2 81 85 162 3.30e-20 91.3
Msa1193950 AT4G36160.2 43.434 99 43 3 2 98 90 177 3.56e-20 91.7
Msa1193950 AT5G53950.1 51.852 81 33 3 2 77 90 169 4.75e-20 90.9
Msa1193950 AT2G27300.1 49.315 73 30 1 2 74 88 153 6.70e-20 90.1
Msa1193950 AT5G17260.1 49.351 77 38 1 2 77 81 157 7.85e-20 91.3
Msa1193950 AT5G62380.2 48.101 79 35 2 2 78 83 157 8.08e-20 90.1
Msa1193950 AT5G62380.1 48.101 79 35 2 2 78 83 157 8.08e-20 90.1
Msa1193950 AT3G03200.1 49.351 77 38 1 2 77 81 157 1.50e-19 90.5
Msa1193950 AT3G15170.1 47.126 87 40 3 2 83 93 178 3.23e-19 87.8
Msa1193950 AT2G18060.2 41.935 93 40 3 8 98 3 83 4.78e-19 86.7
Msa1193950 AT1G69490.1 38.739 111 62 3 2 108 82 190 6.75e-19 86.3
Msa1193950 AT2G33480.3 48.052 77 40 0 2 78 28 104 1.39e-18 84.0
Msa1193950 AT1G01720.1 39.286 112 59 3 2 105 80 190 1.47e-18 85.5
Msa1193950 AT2G33480.2 48.052 77 38 1 2 78 85 159 2.33e-18 84.3
Msa1193950 AT5G46590.1 49.367 79 36 3 2 77 81 158 2.90e-18 84.7
Msa1193950 AT4G27410.2 29.870 154 107 1 2 154 87 240 3.10e-18 84.7
Msa1193950 AT4G27410.3 29.870 154 107 1 2 154 104 257 3.21e-18 85.1
Msa1193950 AT3G61910.1 41.573 89 39 1 2 77 87 175 3.24e-18 85.1
Msa1193950 AT2G33480.1 48.052 77 40 0 2 78 85 161 3.72e-18 84.0
Msa1193950 AT5G22290.1 45.205 73 34 1 2 74 95 161 4.81e-18 84.7
Msa1193950 AT5G66300.1 41.758 91 50 2 2 91 88 176 1.05e-17 83.2
Msa1193950 AT1G52890.1 41.558 77 45 0 2 78 87 163 1.14e-17 83.6
Msa1193950 AT1G54330.1 46.988 83 41 1 2 84 81 160 1.20e-17 83.2
Msa1193950 AT3G44290.2 43.836 73 34 1 2 74 88 153 1.65e-17 81.3
Msa1193950 AT3G15500.1 40.260 77 46 0 2 78 87 163 1.74e-17 82.8
Msa1193950 AT4G17980.1 46.250 80 39 2 2 77 81 160 3.99e-17 80.9
Msa1193950 AT1G32770.1 39.080 87 43 1 2 78 92 178 4.31e-17 82.4
Msa1193950 AT5G22290.2 46.269 67 30 1 8 74 2 62 4.85e-17 80.1
Msa1193950 AT4G28530.1 47.436 78 35 2 2 77 99 172 5.38e-17 82.0
Msa1193950 AT4G17980.2 46.250 80 39 2 2 77 81 160 5.68e-17 80.9
Msa1193950 AT3G44290.1 43.836 73 34 1 2 74 88 153 6.29e-17 81.6
Msa1193950 AT1G77450.1 43.210 81 42 2 2 79 83 162 7.98e-17 79.7
Msa1193950 AT1G32510.1 42.353 85 41 2 1 77 80 164 1.23e-16 79.7
Msa1193950 AT5G46590.2 56.897 58 24 1 2 58 81 138 1.66e-16 79.3
Msa1193950 AT5G08790.1 39.759 83 44 2 2 79 80 161 4.85e-16 78.2
Msa1193950 AT5G63790.2 39.759 83 44 2 2 79 133 214 1.37e-15 77.4
Msa1193950 AT5G63790.1 39.759 83 44 2 2 79 123 204 1.45e-15 77.0
Msa1193950 AT2G43000.2 42.857 77 43 1 2 77 13 89 1.78e-15 74.7
Msa1193950 AT3G15510.1 38.202 89 42 2 2 77 90 178 7.02e-15 75.9
Msa1193950 AT1G61110.1 38.202 89 42 2 2 77 89 177 7.31e-15 75.1
Msa1193950 AT2G02450.1 41.772 79 43 2 2 79 124 200 7.91e-15 75.9
Msa1193950 AT2G43000.1 42.857 77 43 1 2 77 91 167 9.50e-15 74.3
Msa1193950 AT2G02450.2 41.772 79 43 2 2 79 124 200 1.09e-14 75.5
Msa1193950 AT2G17040.1 40.506 79 39 2 2 77 79 152 2.05e-14 73.2
Msa1193950 AT1G26870.1 40.230 87 41 3 2 77 89 175 3.36e-14 73.9
Msa1193950 AT3G04070.2 37.500 88 43 2 2 77 83 170 6.46e-14 72.8
Msa1193950 AT4G01540.3 43.750 80 37 2 1 77 80 154 1.31e-13 72.4
Msa1193950 AT4G01540.4 43.750 80 37 2 1 77 80 154 1.31e-13 72.0
Msa1193950 AT4G01520.1 42.500 80 38 2 1 77 80 154 1.83e-13 70.9
Msa1193950 AT4G01540.2 43.750 80 37 2 1 77 80 154 2.05e-13 71.6
Msa1193950 AT4G01540.1 43.750 80 37 2 1 77 80 154 2.05e-13 72.0
Msa1193950 AT3G04070.1 29.808 104 45 2 2 77 83 186 3.43e-12 67.4
Msa1193950 AT3G12910.1 35.165 91 44 2 2 77 95 185 5.20e-12 66.6
Msa1193950 AT4G01550.1 33.846 130 66 5 3 123 81 199 5.65e-12 67.4
Msa1193950 AT3G44350.2 37.500 80 48 2 2 79 83 162 6.03e-12 65.5
Msa1193950 AT5G39820.1 33.333 99 43 3 2 77 93 191 6.72e-12 66.6
Msa1193950 AT5G04395.1 45.902 61 30 1 1 58 113 173 9.73e-12 63.9
Msa1193950 AT1G52880.1 38.636 88 42 3 2 77 90 177 3.95e-11 63.9
Msa1193950 AT5G22380.1 37.037 81 48 2 2 79 84 164 7.04e-11 62.4

Find 152 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCATATTGTGCACCATTCC+TGG 0.177058 8_1:-40921456 None:intergenic
CTCCAGTCTTCCTCATTAAA+TGG 0.199186 8_1:-40921482 None:intergenic
CTCCATGCTATTGGATATAA+TGG 0.269719 8_1:+40921599 Msa1193950:CDS
CCCAGGAATGGTGCACAATA+TGG 0.271510 8_1:+40921455 Msa1193950:CDS
TGAGCGTCGGACTTAATATT+TGG 0.278214 8_1:+40922640 Msa1193950:three_prime_UTR
CCTATTTGACATTCATTTGC+AGG 0.281187 8_1:-40921890 None:intergenic
CCTTTCCAGTATGAAATATC+AGG 0.282193 8_1:-40920636 None:intergenic
TAAAGACACATTGTTTGAAT+TGG 0.282968 8_1:+40922716 Msa1193950:three_prime_UTR
ATCCAATAGCATGGAGATTC+TGG 0.283199 8_1:-40921592 None:intergenic
TTATCTCCAAAACGGGGATT+TGG 0.288256 8_1:+40920471 Msa1193950:five_prime_UTR
CCTGTTTGACATGAATTTGC+AGG 0.309691 8_1:-40921722 None:intergenic
CCTGCAAATTCATGTCAAAT+AGG 0.314809 8_1:+40921764 Msa1193950:CDS
CCTGTTTGACGTGAATTTGC+AGG 0.316892 8_1:-40921929 None:intergenic
CCTGCAAATGAATGTCAAAT+AGG 0.318011 8_1:+40921890 Msa1193950:CDS
CCTATTTGACATGAATTTGC+AGG 0.318113 8_1:-40921764 None:intergenic
AGTAATGTATTATTGTGATT+TGG 0.319209 8_1:+40922783 Msa1193950:three_prime_UTR
ATGAATCGATCTACGGAAAC+AGG 0.329353 8_1:+40920544 Msa1193950:CDS
ATCTAGACTCCGAGATGTTC+TGG 0.337030 8_1:+40922538 Msa1193950:CDS
GACCAGAATCTCCATGCTAT+TGG 0.337192 8_1:+40921590 Msa1193950:CDS
CCTGTTTGACATGAATTTGC+TGG 0.344593 8_1:-40921668 None:intergenic
ACCACTCCAAATCCCCGTTT+TGG 0.346187 8_1:-40920477 None:intergenic
GTAATGTATTATTGTGATTT+GGG 0.350537 8_1:+40922784 Msa1193950:three_prime_UTR
GATCTACGGAAACAGGGTTT+TGG 0.358106 8_1:+40920551 Msa1193950:CDS
ATTTATCTTATCTCCAAAAC+GGG 0.365480 8_1:+40920464 Msa1193950:five_prime_UTR
CCTTCAAATTCATGTCAAAC+AGG 0.368528 8_1:+40921848 Msa1193950:CDS
AAAGACACATTGTTTGAATT+GGG 0.372063 8_1:+40922717 Msa1193950:three_prime_UTR
TGGCACTATTGCTGCTGTGC+TGG 0.372503 8_1:+40922660 Msa1193950:three_prime_UTR
CCAGAAGGTGATTTGGTCAT+TGG 0.375489 8_1:+40922683 Msa1193950:three_prime_UTR
TGACATTCATTTGCAGGATC+GGG 0.375493 8_1:-40921884 None:intergenic
GGATGACTACTTGGATTTCA+TGG 0.384800 8_1:+40922506 Msa1193950:CDS
TTTGGGAGACCTGGATTGCT+TGG 0.388164 8_1:+40922431 Msa1193950:CDS
AACTACTAAAATATATATAA+AGG 0.389993 8_1:-40922743 None:intergenic
GATTTGGTCATTGGCACATA+TGG 0.392401 8_1:+40922692 Msa1193950:three_prime_UTR
TTGGGAGACCTGGATTGCTT+GGG 0.394189 8_1:+40922432 Msa1193950:CDS
ATGTGCTAAAGTCAGCATTC+TGG 0.394807 8_1:-40922464 None:intergenic
GACTGATAGAGGAATTGTTC+AGG 0.397382 8_1:+40920720 Msa1193950:CDS
CCAATGACCAAATCACCTTC+TGG 0.400154 8_1:-40922683 None:intergenic
CTCGTGGGGATCGTACTGAT+TGG 0.405759 8_1:+40920662 Msa1193950:CDS
GATTTATCTTATCTCCAAAA+CGG 0.426997 8_1:+40920463 Msa1193950:five_prime_UTR
AAGTCAGCATTCTGGCCAAC+AGG 0.430726 8_1:-40922456 None:intergenic
GATAAGGATTTGACTGATAG+AGG 0.435530 8_1:+40920709 Msa1193950:CDS
CCATATTGTGCACCATTCCT+GGG 0.435926 8_1:-40921455 None:intergenic
AAAACCCTGATATTTCATAC+TGG 0.438226 8_1:+40920631 Msa1193950:CDS
TGACATGAATTTGCAGGGTA+GGG 0.440925 8_1:-40921758 None:intergenic
TGACATGAATTTGCAGGGTC+GGG 0.444499 8_1:-40921716 None:intergenic
TGTCCGATAGGGAAGAAGTA+TGG 0.449188 8_1:+40920508 Msa1193950:five_prime_UTR
TGACATGAATTTGCTGGATC+GGG 0.450774 8_1:-40921662 None:intergenic
CCTGTTTGACATGAATTTGA+AGG 0.451294 8_1:-40921848 None:intergenic
CCTGCAAATTCACGTCAAAC+AGG 0.452097 8_1:+40921929 Msa1193950:CDS
GTGCTGGCCAGAAGGTGATT+TGG 0.452395 8_1:+40922676 Msa1193950:three_prime_UTR
TGACGTGAATTTGCAGGGTC+GGG 0.454809 8_1:-40921923 None:intergenic
TGTCAAACAGGATTACAAAC+AGG 0.456291 8_1:+40921680 Msa1193950:CDS
CCAGCAAATTCATGTCAAAC+AGG 0.459332 8_1:+40921668 Msa1193950:CDS
CAACAGGTCCCAAGCAATCC+AGG 0.459478 8_1:-40922440 None:intergenic
CCTGTGAATTCATGTCAAAC+AGG 0.460258 8_1:+40921806 Msa1193950:CDS
TGACATGAATTTGAAGGGTC+GGG 0.460660 8_1:-40921842 None:intergenic
CAACAGAATAATCAAATTGT+GGG 0.461766 8_1:+40920601 Msa1193950:CDS
TGAGTATAGGCTTGAAGATA+AGG 0.462659 8_1:+40920693 Msa1193950:CDS
TTGACATTCATTTGCAGGAT+CGG 0.462975 8_1:-40921885 None:intergenic
CCAGGAATGGTGCACAATAT+GGG 0.464995 8_1:+40921456 Msa1193950:CDS
ATTGGGTTATGCATGAGTAT+AGG 0.467758 8_1:+40920680 Msa1193950:CDS
ATATCCATGCCATGTCAATC+AGG 0.469355 8_1:+40921626 Msa1193950:CDS
CCTGCAAATTCATGTCAAAC+AGG 0.469358 8_1:+40921722 Msa1193950:CDS
AAAAGGAAGGTCCTGGTCCC+AGG 0.470057 8_1:+40921438 Msa1193950:CDS
CACATACGGAATGGCTCCTA+CGG 0.471738 8_1:+40922482 Msa1193950:CDS
CTTTCCTTTGATAATTGTCT+CGG 0.479420 8_1:+40922810 Msa1193950:three_prime_UTR
TTGACGTGAATTTGCAGGGT+CGG 0.485241 8_1:-40921924 None:intergenic
TTGACATGAATTTGCAGGGT+AGG 0.487996 8_1:-40921759 None:intergenic
AGTGCAGCTGGCTCAGCAAA+TGG 0.489746 8_1:-40921524 None:intergenic
AAAGTGGGAGCTAGTGCAGC+TGG 0.489936 8_1:-40921536 None:intergenic
TCGTGGGGATCGTACTGATT+GGG 0.493211 8_1:+40920663 Msa1193950:CDS
AACCATTTAATGAGGAAGAC+TGG 0.493900 8_1:+40921480 Msa1193950:CDS
CTGGATTGCTTGGGACCTGT+TGG 0.494191 8_1:+40922441 Msa1193950:CDS
CCTGTTTGACATGAATTCAC+AGG 0.497735 8_1:-40921806 None:intergenic
AAATTCATGTAAAACAAGAT+TGG 0.499270 8_1:+40921979 Msa1193950:CDS
AATCTGTAAGGTATTTCAAA+AGG 0.505571 8_1:+40921421 Msa1193950:CDS
ATCGTGAATGAACACGCTCT+TGG 0.506536 8_1:+40922836 Msa1193950:three_prime_UTR
CTTCCATACTTCTTCCCTAT+CGG 0.514378 8_1:-40920511 None:intergenic
TTGACATGAATTTGCAGGGT+CGG 0.514389 8_1:-40921717 None:intergenic
TTGACATGAATTTGCTGGAT+CGG 0.514396 8_1:-40921663 None:intergenic
CTGTTTGACATGAATTTGAA+GGG 0.516573 8_1:-40921847 None:intergenic
GAATTTGCAGGGTCGGGAGA+AGG 0.521896 8_1:-40921710 None:intergenic
CTGTTTGACGTGAATTTGCA+GGG 0.521995 8_1:-40921928 None:intergenic
GGATTCCTATGTAATCTGTA+AGG 0.523374 8_1:+40921409 Msa1193950:intron
AGAAGTATGGAAGTGGGTCG+AGG 0.523425 8_1:+40920521 Msa1193950:five_prime_UTR
TCAACAGAATAATCAAATTG+TGG 0.524176 8_1:+40920600 Msa1193950:CDS
TCAGAAAATATCATGCCAAA+AGG 0.525023 8_1:-40922613 None:intergenic
TGGTATTTCTTCTGTCCGAT+AGG 0.528063 8_1:+40920496 Msa1193950:five_prime_UTR
TTGACATGAATTTGAAGGGT+CGG 0.530544 8_1:-40921843 None:intergenic
CTGTTTGACATGAATTTGCA+GGG 0.532638 8_1:-40921721 None:intergenic
GAGTGCTGCGGAACCTAAGT+TGG 0.533048 8_1:+40922563 Msa1193950:CDS
CAATCCTGATTGACATGGCA+TGG 0.533909 8_1:-40921630 None:intergenic
TATGAATTATCCACAGGGTC+GGG 0.534846 8_1:-40922010 None:intergenic
GTGAATGAACACGCTCTTGG+TGG 0.535364 8_1:+40922839 Msa1193950:three_prime_UTR
CATGAATTTGCAGGGCCAGT+GGG 0.536500 8_1:-40921965 None:intergenic
AAATACCTTACAGATTACAT+AGG 0.536956 8_1:-40921414 None:intergenic
AACCATTATATCCAATAGCA+TGG 0.540691 8_1:-40921601 None:intergenic
CGAGACAATTATCAAAGGAA+AGG 0.540867 8_1:-40922809 None:intergenic
GAATTTGCTGGATCGGGAGA+AGG 0.544144 8_1:-40921656 None:intergenic
TTATCCACAGGGTCGGGAGA+AGG 0.544235 8_1:-40922004 None:intergenic
TGATGATATGAATTATCCAC+AGG 0.545478 8_1:-40922016 None:intergenic
TGATGATATGAATTATCCAC+AGG 0.545478 8_1:-40922058 None:intergenic
GAATTTGAAGGGTCGGGAGA+CGG 0.546357 8_1:-40921836 None:intergenic
GGATTGGAGCTGTCTCCCAC+TGG 0.549474 8_1:+40921950 Msa1193950:CDS
CTTTCCAGTATGAAATATCA+GGG 0.551975 8_1:-40920635 None:intergenic
GGTATTTCTTCTGTCCGATA+GGG 0.553898 8_1:+40920497 Msa1193950:five_prime_UTR
AATGCTGACTTTAGCACATA+CGG 0.554890 8_1:+40922468 Msa1193950:CDS
TGTAAGGTATTTCAAAAGGA+AGG 0.556870 8_1:+40921425 Msa1193950:CDS
ACATACGGAATGGCTCCTAC+GGG 0.561143 8_1:+40922483 Msa1193950:CDS
GAAGGTCCTGGTCCCAGGAA+TGG 0.561485 8_1:+40921443 Msa1193950:CDS
GAATTTGCAGGGTAGGGAGA+AGG 0.564019 8_1:-40921752 None:intergenic
GACTGGAGTGATGATGAAGT+AGG 0.564465 8_1:+40921497 Msa1193950:CDS
TCATTTGCAGGATCGGGAGA+CGG 0.564896 8_1:-40921878 None:intergenic
TTGCTGCTGTGCTGGCCAGA+AGG 0.573325 8_1:+40922668 Msa1193950:three_prime_UTR
ACATGAATTTGCAGGGCCAG+TGG 0.576347 8_1:-40921966 None:intergenic
GGCCAGGAAAACAATGCTGA+AGG 0.577250 8_1:+40922381 Msa1193950:intron
AAATTCACGTCAAACAGGAT+TGG 0.579546 8_1:+40921934 Msa1193950:CDS
ATATGAATTATCCACAGGGT+CGG 0.580394 8_1:-40922011 None:intergenic
ATAGGGAAGAAGTATGGAAG+TGG 0.581783 8_1:+40920514 Msa1193950:five_prime_UTR
GTATTTCAAAAGGAAGGTCC+TGG 0.582263 8_1:+40921431 Msa1193950:CDS
ATACGGAATGGCTCCTACGG+GGG 0.584987 8_1:+40922485 Msa1193950:CDS
TTAGATGTCACAGGCAAAGT+GGG 0.587463 8_1:-40921551 None:intergenic
TACTGGAAAGGCACCTCGTG+GGG 0.589079 8_1:+40920648 Msa1193950:CDS
ACAAAACTATTAGATGTCAC+AGG 0.590588 8_1:-40921560 None:intergenic
CTGTTTGACATGAATTCACA+GGG 0.594081 8_1:-40921805 None:intergenic
CCTGATATTTCATACTGGAA+AGG 0.594945 8_1:+40920636 Msa1193950:CDS
CTATTTGACATGAATTTGCA+GGG 0.594947 8_1:-40921763 None:intergenic
ATATGGGAAACCATTTAATG+AGG 0.595239 8_1:+40921472 Msa1193950:CDS
AGCACTCTGCCAGAACATCT+CGG 0.596496 8_1:-40922547 None:intergenic
TTTGCAGGGTAGGGAGAAGG+TGG 0.603489 8_1:-40921749 None:intergenic
ATTAGATGTCACAGGCAAAG+TGG 0.604507 8_1:-40921552 None:intergenic
TGTGCACCATTCCTGGGACC+AGG 0.606475 8_1:-40921449 None:intergenic
TGACTTTAGCACATACGGAA+TGG 0.608742 8_1:+40922473 Msa1193950:CDS
GTCGAGGATGAATCGATCTA+CGG 0.611940 8_1:+40920537 Msa1193950:exon
GGCATCAATCCTGATTGACA+TGG 0.616405 8_1:-40921635 None:intergenic
TTTGCAGGGTCGGGAGAAGG+CGG 0.617658 8_1:-40921707 None:intergenic
ATACTGGAAAGGCACCTCGT+GGG 0.623402 8_1:+40920647 Msa1193950:CDS
TCCAAAACGGGGATTTGGAG+TGG 0.632470 8_1:+40920476 Msa1193950:five_prime_UTR
GAATTCACAGGGTCTACAGA+AGG 0.634919 8_1:-40921794 None:intergenic
AACAGAATAATCAAATTGTG+GGG 0.640227 8_1:+40920602 Msa1193950:CDS
CATACTGGAAAGGCACCTCG+TGG 0.642917 8_1:+40920646 Msa1193950:CDS
CTTCAAATTCATGTCAAACA+GGG 0.645767 8_1:+40921849 Msa1193950:CDS
TGAATCGATCTACGGAAACA+GGG 0.649117 8_1:+40920545 Msa1193950:CDS
GATGTTCTGGCAGAGTGCTG+CGG 0.652067 8_1:+40922551 Msa1193950:CDS
TAGGGAAGAAGTATGGAAGT+GGG 0.665858 8_1:+40920515 Msa1193950:five_prime_UTR
TTTATCTTATCTCCAAAACG+GGG 0.680682 8_1:+40920465 Msa1193950:five_prime_UTR
CATACGGAATGGCTCCTACG+GGG 0.702272 8_1:+40922484 Msa1193950:CDS
CGACGACGACATATTGCCTG+AGG 0.702294 8_1:+40922123 Msa1193950:CDS
GATGATATGAATTATCCACA+GGG 0.739152 8_1:-40922057 None:intergenic
GATGATATGAATTATCCACA+GGG 0.748736 8_1:-40922015 None:intergenic
CAATCAGTACGATCCCCACG+AGG 0.773083 8_1:-40920661 None:intergenic
TGTTAATAATGATGACCCTG+TGG 0.779783 8_1:+40922042 Msa1193950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGAAAAATTCTTTATTTT+AGG - chr8_1:40922590-40922609 None:intergenic 10.0%
!!! TAAAATAAAGAATTTTTCAT+TGG + chr8_1:40922589-40922608 Msa1193950:exon 10.0%
!! AACTACTAAAATATATATAA+AGG - chr8_1:40922746-40922765 None:intergenic 10.0%
!!! AATAACATATTTCTATTTCA+AGG - chr8_1:40921129-40921148 None:intergenic 15.0%
!!! ATAGAAATATGTTATTTCAT+TGG + chr8_1:40921133-40921152 Msa1193950:intron 15.0%
!! TAAAAAAAAAATTCCAAACA+AGG - chr8_1:40922945-40922964 None:intergenic 15.0%
!!! AATTACTATGAGCTTTATTA+GGG + chr8_1:40920778-40920797 Msa1193950:intron 20.0%
!! GATTCATAGATATATTACAT+TGG + chr8_1:40920961-40920980 Msa1193950:intron 20.0%
!! ATTAACATGATTCTATTTCA+AGG - chr8_1:40921227-40921246 None:intergenic 20.0%
!! ATGATTAAAATATGCAAGAA+GGG - chr8_1:40921309-40921328 None:intergenic 20.0%
!! AATGATTAAAATATGCAAGA+AGG - chr8_1:40921310-40921329 None:intergenic 20.0%
!!! GCATATTTTAATCATTTGAT+TGG + chr8_1:40921314-40921333 Msa1193950:intron 20.0%
!! AAATTCATGTAAAACAAGAT+TGG + chr8_1:40921979-40921998 Msa1193950:CDS 20.0%
!!! TGATGTTTCTTTTTTATGTA+TGG + chr8_1:40922202-40922221 Msa1193950:intron 20.0%
!! ATCACAATCATTTAGTAAAA+TGG - chr8_1:40922307-40922326 None:intergenic 20.0%
!! AGTAATGTATTATTGTGATT+TGG + chr8_1:40922783-40922802 Msa1193950:three_prime_UTR 20.0%
!! GTAATGTATTATTGTGATTT+GGG + chr8_1:40922784-40922803 Msa1193950:three_prime_UTR 20.0%
! GATTTATCTTATCTCCAAAA+CGG + chr8_1:40920463-40920482 Msa1193950:five_prime_UTR 25.0%
! ATTTATCTTATCTCCAAAAC+GGG + chr8_1:40920464-40920483 Msa1193950:five_prime_UTR 25.0%
! TCAACAGAATAATCAAATTG+TGG + chr8_1:40920600-40920619 Msa1193950:CDS 25.0%
! CAACAGAATAATCAAATTGT+GGG + chr8_1:40920601-40920620 Msa1193950:CDS 25.0%
! AACAGAATAATCAAATTGTG+GGG + chr8_1:40920602-40920621 Msa1193950:CDS 25.0%
!!! GAATTACTATGAGCTTTATT+AGG + chr8_1:40920777-40920796 Msa1193950:intron 25.0%
!!! TAAGTTAGCATTTTGATATG+TGG + chr8_1:40920808-40920827 Msa1193950:intron 25.0%
!! AATTTTGAATGCACATGTAT+AGG + chr8_1:40921098-40921117 Msa1193950:intron 25.0%
!! ATTTTGAATGCACATGTATA+GGG + chr8_1:40921099-40921118 Msa1193950:intron 25.0%
! AGTAAAATATGCCCTTTATA+AGG - chr8_1:40921194-40921213 None:intergenic 25.0%
!! AAAACAGCAAATCTCATTTT+GGG - chr8_1:40921267-40921286 None:intergenic 25.0%
!! AAAAACAGCAAATCTCATTT+TGG - chr8_1:40921268-40921287 None:intergenic 25.0%
! AATGTATTCACAATGAAAAC+AGG - chr8_1:40921352-40921371 None:intergenic 25.0%
!! ACATTTTTAACAAGCTCTTT+TGG + chr8_1:40921367-40921386 Msa1193950:intron 25.0%
! AAATACCTTACAGATTACAT+AGG - chr8_1:40921417-40921436 None:intergenic 25.0%
!! AATCTGTAAGGTATTTCAAA+AGG + chr8_1:40921421-40921440 Msa1193950:CDS 25.0%
!!! TTGTTTTACATGAATTTGCA+GGG - chr8_1:40921976-40921995 None:intergenic 25.0%
! GAAAACAAAAAAACACAAGA+AGG - chr8_1:40922170-40922189 None:intergenic 25.0%
!!! CTTGTGTTTTTTTGTTTTCT+TGG + chr8_1:40922171-40922190 Msa1193950:intron 25.0%
! AAAATGGCATTAACATTATG+AGG - chr8_1:40922291-40922310 None:intergenic 25.0%
!! CTGAATACTGACATTATTTT+TGG + chr8_1:40922360-40922379 Msa1193950:intron 25.0%
! TAAAGACACATTGTTTGAAT+TGG + chr8_1:40922716-40922735 Msa1193950:three_prime_UTR 25.0%
! AAAGACACATTGTTTGAATT+GGG + chr8_1:40922717-40922736 Msa1193950:three_prime_UTR 25.0%
! GAAAAAATGTATGCACAAAT+CGG - chr8_1:40922914-40922933 None:intergenic 25.0%
!! TTTTCACTTTAAACCTTGTT+TGG + chr8_1:40922929-40922948 Msa1193950:three_prime_UTR 25.0%
TTTATCTTATCTCCAAAACG+GGG + chr8_1:40920465-40920484 Msa1193950:five_prime_UTR 30.0%
AAAACCCTGATATTTCATAC+TGG + chr8_1:40920631-40920650 Msa1193950:CDS 30.0%
CTTTCCAGTATGAAATATCA+GGG - chr8_1:40920638-40920657 None:intergenic 30.0%
!! ATTCTTAGTGTTGATGCTAT+CGG + chr8_1:40920881-40920900 Msa1193950:intron 30.0%
!!! GCATTTTAAAGTTTGAAGTG+TGG + chr8_1:40921044-40921063 Msa1193950:intron 30.0%
! TTTTGAATGCACATGTATAG+GGG + chr8_1:40921100-40921119 Msa1193950:intron 30.0%
TGATTCTCCTTACCTTATAA+AGG + chr8_1:40921179-40921198 Msa1193950:intron 30.0%
GATTCTCCTTACCTTATAAA+GGG + chr8_1:40921180-40921199 Msa1193950:intron 30.0%
AATATGCCCTTTATAAGGTA+AGG - chr8_1:40921189-40921208 None:intergenic 30.0%
!!! CTGTTTTTACATGATGAAGT+TGG + chr8_1:40921280-40921299 Msa1193950:intron 30.0%
TATTCACAATGAAAACAGGA+CGG - chr8_1:40921348-40921367 None:intergenic 30.0%
! GGTTTGTCTTTTTAAATCAC+AGG + chr8_1:40921388-40921407 Msa1193950:intron 30.0%
! TGTAAGGTATTTCAAAAGGA+AGG + chr8_1:40921425-40921444 Msa1193950:CDS 30.0%
ATATGGGAAACCATTTAATG+AGG + chr8_1:40921472-40921491 Msa1193950:CDS 30.0%
ACAAAACTATTAGATGTCAC+AGG - chr8_1:40921563-40921582 None:intergenic 30.0%
AACCATTATATCCAATAGCA+TGG - chr8_1:40921604-40921623 None:intergenic 30.0%
CTATTTGACATGAATTTGCA+GGG - chr8_1:40921766-40921785 None:intergenic 30.0%
CTGTTTGACATGAATTTGAA+GGG - chr8_1:40921850-40921869 None:intergenic 30.0%
CTTCAAATTCATGTCAAACA+GGG + chr8_1:40921849-40921868 Msa1193950:CDS 30.0%
!!! CTTGTTTTACATGAATTTGC+AGG - chr8_1:40921977-40921996 None:intergenic 30.0%
GATGATATGAATTATCCACA+GGG - chr8_1:40922018-40922037 None:intergenic 30.0%
TGATGATATGAATTATCCAC+AGG - chr8_1:40922019-40922038 None:intergenic 30.0%
GATGATATGAATTATCCACA+GGG - chr8_1:40922018-40922037 None:intergenic 30.0%
TGATGATATGAATTATCCAC+AGG - chr8_1:40922019-40922038 None:intergenic 30.0%
! TTAGTGTTTTCTACTCTTGT+AGG + chr8_1:40922227-40922246 Msa1193950:intron 30.0%
!!! CTATCAACTGACTTTTTTGA+TGG + chr8_1:40922408-40922427 Msa1193950:CDS 30.0%
!!! AACTGACTTTTTTGATGGTT+TGG + chr8_1:40922413-40922432 Msa1193950:CDS 30.0%
!!! ACTGACTTTTTTGATGGTTT+GGG + chr8_1:40922414-40922433 Msa1193950:CDS 30.0%
!!! CTTTATTTTAGGTCCAACTT+AGG - chr8_1:40922579-40922598 None:intergenic 30.0%
! GAATTTTTCATTGGTCCTTT+TGG + chr8_1:40922598-40922617 Msa1193950:three_prime_UTR 30.0%
TCAGAAAATATCATGCCAAA+AGG - chr8_1:40922616-40922635 None:intergenic 30.0%
! CTTTCCTTTGATAATTGTCT+CGG + chr8_1:40922810-40922829 Msa1193950:three_prime_UTR 30.0%
! TTTTCCGAGACAATTATCAA+AGG - chr8_1:40922817-40922836 None:intergenic 30.0%
CCTTTCCAGTATGAAATATC+AGG - chr8_1:40920639-40920658 None:intergenic 35.0%
CCTGATATTTCATACTGGAA+AGG + chr8_1:40920636-40920655 Msa1193950:CDS 35.0%
ATTGGGTTATGCATGAGTAT+AGG + chr8_1:40920680-40920699 Msa1193950:CDS 35.0%
TGAGTATAGGCTTGAAGATA+AGG + chr8_1:40920693-40920712 Msa1193950:CDS 35.0%
GATAAGGATTTGACTGATAG+AGG + chr8_1:40920709-40920728 Msa1193950:CDS 35.0%
TTATGTGCATCCACAATCAA+AGG - chr8_1:40920850-40920869 None:intergenic 35.0%
AATGCCATTAGTGACATAGT+AGG - chr8_1:40921001-40921020 None:intergenic 35.0%
GGATTCCTATGTAATCTGTA+AGG + chr8_1:40921409-40921428 Msa1193950:intron 35.0%
AACCATTTAATGAGGAAGAC+TGG + chr8_1:40921480-40921499 Msa1193950:CDS 35.0%
CTCCATGCTATTGGATATAA+TGG + chr8_1:40921599-40921618 Msa1193950:CDS 35.0%
!! TTGACATGAATTTGCTGGAT+CGG - chr8_1:40921666-40921685 None:intergenic 35.0%
TGTCAAACAGGATTACAAAC+AGG + chr8_1:40921680-40921699 Msa1193950:CDS 35.0%
CTGTTTGACATGAATTTGCA+GGG - chr8_1:40921724-40921743 None:intergenic 35.0%
CCTATTTGACATGAATTTGC+AGG - chr8_1:40921767-40921786 None:intergenic 35.0%
CCTGCAAATTCATGTCAAAT+AGG + chr8_1:40921764-40921783 Msa1193950:CDS 35.0%
CTGTTTGACATGAATTCACA+GGG - chr8_1:40921808-40921827 None:intergenic 35.0%
TTGACATGAATTTGAAGGGT+CGG - chr8_1:40921846-40921865 None:intergenic 35.0%
CCTGTTTGACATGAATTTGA+AGG - chr8_1:40921851-40921870 None:intergenic 35.0%
CCTTCAAATTCATGTCAAAC+AGG + chr8_1:40921848-40921867 Msa1193950:CDS 35.0%
TTGACATTCATTTGCAGGAT+CGG - chr8_1:40921888-40921907 None:intergenic 35.0%
CCTATTTGACATTCATTTGC+AGG - chr8_1:40921893-40921912 None:intergenic 35.0%
CCTGCAAATGAATGTCAAAT+AGG + chr8_1:40921890-40921909 Msa1193950:CDS 35.0%
AAATTCACGTCAAACAGGAT+TGG + chr8_1:40921934-40921953 Msa1193950:CDS 35.0%
ATATGAATTATCCACAGGGT+CGG - chr8_1:40922014-40922033 None:intergenic 35.0%
TGTTAATAATGATGACCCTG+TGG + chr8_1:40922042-40922061 Msa1193950:CDS 35.0%
AGCGACTCTTGATTTCTTTA+AGG + chr8_1:40922333-40922352 Msa1193950:intron 35.0%
!!! TACTGACATTATTTTTGGCC+AGG + chr8_1:40922365-40922384 Msa1193950:intron 35.0%
!! AATGCTGACTTTAGCACATA+CGG + chr8_1:40922468-40922487 Msa1193950:CDS 35.0%
CGAGACAATTATCAAAGGAA+AGG - chr8_1:40922812-40922831 None:intergenic 35.0%
TTATCTCCAAAACGGGGATT+TGG + chr8_1:40920471-40920490 Msa1193950:five_prime_UTR 40.0%
TGGTATTTCTTCTGTCCGAT+AGG + chr8_1:40920496-40920515 Msa1193950:five_prime_UTR 40.0%
GGTATTTCTTCTGTCCGATA+GGG + chr8_1:40920497-40920516 Msa1193950:five_prime_UTR 40.0%
CTTCCATACTTCTTCCCTAT+CGG - chr8_1:40920514-40920533 None:intergenic 40.0%
! ATAGGGAAGAAGTATGGAAG+TGG + chr8_1:40920514-40920533 Msa1193950:five_prime_UTR 40.0%
! TAGGGAAGAAGTATGGAAGT+GGG + chr8_1:40920515-40920534 Msa1193950:five_prime_UTR 40.0%
ATGAATCGATCTACGGAAAC+AGG + chr8_1:40920544-40920563 Msa1193950:CDS 40.0%
TGAATCGATCTACGGAAACA+GGG + chr8_1:40920545-40920564 Msa1193950:CDS 40.0%
!!! GTTTTGGAAAGCTACTGGTA+AGG + chr8_1:40920567-40920586 Msa1193950:CDS 40.0%
GACTGATAGAGGAATTGTTC+AGG + chr8_1:40920720-40920739 Msa1193950:CDS 40.0%
TGAAGTTGTGCCTTTGATTG+TGG + chr8_1:40920837-40920856 Msa1193950:intron 40.0%
TCCACCTACTATGTCACTAA+TGG + chr8_1:40920994-40921013 Msa1193950:intron 40.0%
! GCATTGACCTCTTCTAAATG+CGG + chr8_1:40921016-40921035 Msa1193950:intron 40.0%
CATTGACCTCTTCTAAATGC+GGG + chr8_1:40921017-40921036 Msa1193950:intron 40.0%
ATTGACCTCTTCTAAATGCG+GGG + chr8_1:40921018-40921037 Msa1193950:intron 40.0%
GTATTTCAAAAGGAAGGTCC+TGG + chr8_1:40921431-40921450 Msa1193950:CDS 40.0%
CTCCAGTCTTCCTCATTAAA+TGG - chr8_1:40921485-40921504 None:intergenic 40.0%
TTAGATGTCACAGGCAAAGT+GGG - chr8_1:40921554-40921573 None:intergenic 40.0%
ATTAGATGTCACAGGCAAAG+TGG - chr8_1:40921555-40921574 None:intergenic 40.0%
ATCCAATAGCATGGAGATTC+TGG - chr8_1:40921595-40921614 None:intergenic 40.0%
ATATCCATGCCATGTCAATC+AGG + chr8_1:40921626-40921645 Msa1193950:CDS 40.0%
!! TGACATGAATTTGCTGGATC+GGG - chr8_1:40921665-40921684 None:intergenic 40.0%
CCTGTTTGACATGAATTTGC+TGG - chr8_1:40921671-40921690 None:intergenic 40.0%
CCAGCAAATTCATGTCAAAC+AGG + chr8_1:40921668-40921687 Msa1193950:CDS 40.0%
TTGACATGAATTTGCAGGGT+CGG - chr8_1:40921720-40921739 None:intergenic 40.0%
CCTGTTTGACATGAATTTGC+AGG - chr8_1:40921671-40921690 None:intergenic 40.0%
CCTGCAAATTCATGTCAAAC+AGG + chr8_1:40921722-40921741 Msa1193950:CDS 40.0%
TGACATGAATTTGCAGGGTA+GGG - chr8_1:40921761-40921780 None:intergenic 40.0%
TTGACATGAATTTGCAGGGT+AGG - chr8_1:40921720-40921739 None:intergenic 40.0%
CCTGTTTGACATGAATTCAC+AGG - chr8_1:40921809-40921828 None:intergenic 40.0%
CCTGTGAATTCATGTCAAAC+AGG + chr8_1:40921806-40921825 Msa1193950:CDS 40.0%
TGACATGAATTTGAAGGGTC+GGG - chr8_1:40921845-40921864 None:intergenic 40.0%
TGACATTCATTTGCAGGATC+GGG - chr8_1:40921887-40921906 None:intergenic 40.0%
CTGTTTGACGTGAATTTGCA+GGG - chr8_1:40921931-40921950 None:intergenic 40.0%
TATGAATTATCCACAGGGTC+GGG - chr8_1:40922013-40922032 None:intergenic 40.0%
GAAACAGATAAGCATACCTC+AGG - chr8_1:40922142-40922161 None:intergenic 40.0%
ATGTGCTAAAGTCAGCATTC+TGG - chr8_1:40922467-40922486 None:intergenic 40.0%
TGACTTTAGCACATACGGAA+TGG + chr8_1:40922473-40922492 Msa1193950:CDS 40.0%
GGATGACTACTTGGATTTCA+TGG + chr8_1:40922506-40922525 Msa1193950:CDS 40.0%
!! TGAGCGTCGGACTTAATATT+TGG + chr8_1:40922640-40922659 Msa1193950:three_prime_UTR 40.0%
! GATTTGGTCATTGGCACATA+TGG + chr8_1:40922692-40922711 Msa1193950:three_prime_UTR 40.0%
TGTCCGATAGGGAAGAAGTA+TGG + chr8_1:40920508-40920527 Msa1193950:five_prime_UTR 45.0%
GTCGAGGATGAATCGATCTA+CGG + chr8_1:40920537-40920556 Msa1193950:exon 45.0%
! GATCTACGGAAACAGGGTTT+TGG + chr8_1:40920551-40920570 Msa1193950:CDS 45.0%
!!! ACAGGGTTTTGGAAAGCTAC+TGG + chr8_1:40920562-40920581 Msa1193950:CDS 45.0%
GCCATTAGTGACATAGTAGG+TGG - chr8_1:40920998-40921017 None:intergenic 45.0%
CCATATTGTGCACCATTCCT+GGG - chr8_1:40921458-40921477 None:intergenic 45.0%
CCAGGAATGGTGCACAATAT+GGG + chr8_1:40921456-40921475 Msa1193950:CDS 45.0%
! GACTGGAGTGATGATGAAGT+AGG + chr8_1:40921497-40921516 Msa1193950:CDS 45.0%
GACCAGAATCTCCATGCTAT+TGG + chr8_1:40921590-40921609 Msa1193950:CDS 45.0%
CAATCCTGATTGACATGGCA+TGG - chr8_1:40921633-40921652 None:intergenic 45.0%
GGCATCAATCCTGATTGACA+TGG - chr8_1:40921638-40921657 None:intergenic 45.0%
TGACATGAATTTGCAGGGTC+GGG - chr8_1:40921719-40921738 None:intergenic 45.0%
GAATTCACAGGGTCTACAGA+AGG - chr8_1:40921797-40921816 None:intergenic 45.0%
TTGACGTGAATTTGCAGGGT+CGG - chr8_1:40921927-40921946 None:intergenic 45.0%
CCTGTTTGACGTGAATTTGC+AGG - chr8_1:40921932-40921951 None:intergenic 45.0%
CCTGCAAATTCACGTCAAAC+AGG + chr8_1:40921929-40921948 Msa1193950:CDS 45.0%
AGGAGACAATGCAAACTGCA+GGG - chr8_1:40922271-40922290 None:intergenic 45.0%
! CACCTTCAGCATTGTTTTCC+TGG - chr8_1:40922386-40922405 None:intergenic 45.0%
!! TTTTGATGGTTTGGGAGACC+TGG + chr8_1:40922422-40922441 Msa1193950:CDS 45.0%
ATCTAGACTCCGAGATGTTC+TGG + chr8_1:40922538-40922557 Msa1193950:CDS 45.0%
! ATTTTCTGACGACTGAGCGT+CGG + chr8_1:40922627-40922646 Msa1193950:three_prime_UTR 45.0%
CCAATGACCAAATCACCTTC+TGG - chr8_1:40922686-40922705 None:intergenic 45.0%
!! CCAGAAGGTGATTTGGTCAT+TGG + chr8_1:40922683-40922702 Msa1193950:three_prime_UTR 45.0%
ATCGTGAATGAACACGCTCT+TGG + chr8_1:40922836-40922855 Msa1193950:three_prime_UTR 45.0%
TCCAAAACGGGGATTTGGAG+TGG + chr8_1:40920476-40920495 Msa1193950:five_prime_UTR 50.0%
! ACCACTCCAAATCCCCGTTT+TGG - chr8_1:40920480-40920499 None:intergenic 50.0%
! AGAAGTATGGAAGTGGGTCG+AGG + chr8_1:40920521-40920540 Msa1193950:five_prime_UTR 50.0%
!! ATACTGGAAAGGCACCTCGT+GGG + chr8_1:40920647-40920666 Msa1193950:CDS 50.0%
!! TCGTGGGGATCGTACTGATT+GGG + chr8_1:40920663-40920682 Msa1193950:CDS 50.0%
GCAATCCCCGCATTTAGAAG+AGG - chr8_1:40921026-40921045 None:intergenic 50.0%
CCCAGGAATGGTGCACAATA+TGG + chr8_1:40921455-40921474 Msa1193950:CDS 50.0%
CCCATATTGTGCACCATTCC+TGG - chr8_1:40921459-40921478 None:intergenic 50.0%
!! GAATTTGCTGGATCGGGAGA+AGG - chr8_1:40921659-40921678 None:intergenic 50.0%
GAATTTGCAGGGTAGGGAGA+AGG - chr8_1:40921755-40921774 None:intergenic 50.0%
GAATTTGAAGGGTCGGGAGA+CGG - chr8_1:40921839-40921858 None:intergenic 50.0%
TCATTTGCAGGATCGGGAGA+CGG - chr8_1:40921881-40921900 None:intergenic 50.0%
TGACGTGAATTTGCAGGGTC+GGG - chr8_1:40921926-40921945 None:intergenic 50.0%
CATGAATTTGCAGGGCCAGT+GGG - chr8_1:40921968-40921987 None:intergenic 50.0%
ACATGAATTTGCAGGGCCAG+TGG - chr8_1:40921969-40921988 None:intergenic 50.0%
GGAGACAATGCAAACTGCAG+GGG - chr8_1:40922270-40922289 None:intergenic 50.0%
GAGGAGACAATGCAAACTGC+AGG - chr8_1:40922272-40922291 None:intergenic 50.0%
GGCCAGGAAAACAATGCTGA+AGG + chr8_1:40922381-40922400 Msa1193950:intron 50.0%
TTTGGGAGACCTGGATTGCT+TGG + chr8_1:40922431-40922450 Msa1193950:CDS 50.0%
TTGGGAGACCTGGATTGCTT+GGG + chr8_1:40922432-40922451 Msa1193950:CDS 50.0%
AAGTCAGCATTCTGGCCAAC+AGG - chr8_1:40922459-40922478 None:intergenic 50.0%
CACATACGGAATGGCTCCTA+CGG + chr8_1:40922482-40922501 Msa1193950:CDS 50.0%
ACATACGGAATGGCTCCTAC+GGG + chr8_1:40922483-40922502 Msa1193950:CDS 50.0%
AGCACTCTGCCAGAACATCT+CGG - chr8_1:40922550-40922569 None:intergenic 50.0%
GTGAATGAACACGCTCTTGG+TGG + chr8_1:40922839-40922858 Msa1193950:three_prime_UTR 50.0%
!! CATACTGGAAAGGCACCTCG+TGG + chr8_1:40920646-40920665 Msa1193950:CDS 55.0%
!! TACTGGAAAGGCACCTCGTG+GGG + chr8_1:40920648-40920667 Msa1193950:CDS 55.0%
CAATCAGTACGATCCCCACG+AGG - chr8_1:40920664-40920683 None:intergenic 55.0%
! CTCGTGGGGATCGTACTGAT+TGG + chr8_1:40920662-40920681 Msa1193950:CDS 55.0%
AAAAGGAAGGTCCTGGTCCC+AGG + chr8_1:40921438-40921457 Msa1193950:CDS 55.0%
! AGTGCAGCTGGCTCAGCAAA+TGG - chr8_1:40921527-40921546 None:intergenic 55.0%
AAAGTGGGAGCTAGTGCAGC+TGG - chr8_1:40921539-40921558 None:intergenic 55.0%
GAATTTGCAGGGTCGGGAGA+AGG - chr8_1:40921713-40921732 None:intergenic 55.0%
TTTGCAGGGTAGGGAGAAGG+TGG - chr8_1:40921752-40921771 None:intergenic 55.0%
TTATCCACAGGGTCGGGAGA+AGG - chr8_1:40922007-40922026 None:intergenic 55.0%
CGACGACGACATATTGCCTG+AGG + chr8_1:40922123-40922142 Msa1193950:CDS 55.0%
CAACAGGTCCCAAGCAATCC+AGG - chr8_1:40922443-40922462 None:intergenic 55.0%
CTGGATTGCTTGGGACCTGT+TGG + chr8_1:40922441-40922460 Msa1193950:CDS 55.0%
CATACGGAATGGCTCCTACG+GGG + chr8_1:40922484-40922503 Msa1193950:CDS 55.0%
ATACGGAATGGCTCCTACGG+GGG + chr8_1:40922485-40922504 Msa1193950:CDS 55.0%
TCCTACGGGGGATGACTACT+TGG + chr8_1:40922497-40922516 Msa1193950:CDS 55.0%
TCCAAGTAGTCATCCCCCGT+AGG - chr8_1:40922501-40922520 None:intergenic 55.0%
!! GATGTTCTGGCAGAGTGCTG+CGG + chr8_1:40922551-40922570 Msa1193950:CDS 55.0%
! GAGTGCTGCGGAACCTAAGT+TGG + chr8_1:40922563-40922582 Msa1193950:CDS 55.0%
TGGCACTATTGCTGCTGTGC+TGG + chr8_1:40922660-40922679 Msa1193950:three_prime_UTR 55.0%
! GTGCTGGCCAGAAGGTGATT+TGG + chr8_1:40922676-40922695 Msa1193950:three_prime_UTR 55.0%
GAAGGTCCTGGTCCCAGGAA+TGG + chr8_1:40921443-40921462 Msa1193950:CDS 60.0%
TGTGCACCATTCCTGGGACC+AGG - chr8_1:40921452-40921471 None:intergenic 60.0%
TTTGCAGGGTCGGGAGAAGG+CGG - chr8_1:40921710-40921729 None:intergenic 60.0%
GGATTGGAGCTGTCTCCCAC+TGG + chr8_1:40921950-40921969 Msa1193950:CDS 60.0%
! TTGCTGCTGTGCTGGCCAGA+AGG + chr8_1:40922668-40922687 Msa1193950:three_prime_UTR 60.0%
GGTGCCTTCTCCCGACCCTG+TGG + chr8_1:40922000-40922019 Msa1193950:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 40920461 40922981 40920461 ID=Msa1193950;Name=Msa1193950
chr8_1 mRNA 40920461 40922981 40920461 ID=Msa1193950-mRNA-1;Parent=Msa1193950;Name=Msa1193950-mRNA-1;_AED=0.07;_eAED=0.07;_QI=83|1|1|1|1|1|3|385|380
chr8_1 exon 40920461 40920741 40920461 ID=Msa1193950-mRNA-1:exon:13210;Parent=Msa1193950-mRNA-1
chr8_1 exon 40921410 40922144 40921410 ID=Msa1193950-mRNA-1:exon:13211;Parent=Msa1193950-mRNA-1
chr8_1 exon 40922387 40922981 40922387 ID=Msa1193950-mRNA-1:exon:13212;Parent=Msa1193950-mRNA-1
chr8_1 five_prime_UTR 40920461 40920543 40920461 ID=Msa1193950-mRNA-1:five_prime_utr;Parent=Msa1193950-mRNA-1
chr8_1 CDS 40920544 40920741 40920544 ID=Msa1193950-mRNA-1:cds;Parent=Msa1193950-mRNA-1
chr8_1 CDS 40921410 40922144 40921410 ID=Msa1193950-mRNA-1:cds;Parent=Msa1193950-mRNA-1
chr8_1 CDS 40922387 40922596 40922387 ID=Msa1193950-mRNA-1:cds;Parent=Msa1193950-mRNA-1
chr8_1 three_prime_UTR 40922597 40922981 40922597 ID=Msa1193950-mRNA-1:three_prime_utr;Parent=Msa1193950-mRNA-1
Gene Sequence

>Msa1193950

ATGAATCGATCTACGGAAACAGGGTTTTGGAAAGCTACTGGTAAGGATAGAGCTGTTCAACAGAATAATCAAATTGTGGGGATGATTAAAACCCTGATATTTCATACTGGAAAGGCACCTCGTGGGGATCGTACTGATTGGGTTATGCATGAGTATAGGCTTGAAGATAAGGATTTGACTGATAGAGGAATTGTTCAGGATTCCTATGTAATCTGTAAGGTATTTCAAAAGGAAGGTCCTGGTCCCAGGAATGGTGCACAATATGGGAAACCATTTAATGAGGAAGACTGGAGTGATGATGAAGTAGGAATACCATTTGCTGAGCCAGCTGCACTAGCTCCCACTTTGCCTGTGACATCTAATAGTTTTGTTCTAAATGACCAGAATCTCCATGCTATTGGATATAATGGTTCAATATCCATGCCATGTCAATCAGGATTGATGCCTTCTCCCGATCCAGCAAATTCATGTCAAACAGGATTACAAACAGGATTACCGCCTTCTCCCGACCCTGCAAATTCATGTCAAACAGGAGTTCCACCTTCTCCCTACCCTGCAAATTCATGTCAAATAGGATTGACGCCTTCTGTAGACCCTGTGAATTCATGTCAAACAGGATTTATGCCGTCTCCCGACCCTTCAAATTCATGTCAAACAGGGTATATGCCGTCTCCCGATCCTGCAAATGAATGTCAAATAGGATTTATGTCTCCCGACCCTGCAAATTCACGTCAAACAGGATTGGAGCTGTCTCCCACTGGCCCTGCAAATTCATGTAAAACAAGATTGGTGCCTTCTCCCGACCCTGTGGATAATTCATATCATCAAGCTGTTAATAATGATGACCCTGTGGATAATTCATATCATCAAGCTGTTAATAATGATGACATTTTATCAATGCTTGATATGTTCGACGACGACATATTGCCTGAGGAAAACAATGCTGAAGGTGCACTATCAACTGACTTTTTTGATGGTTTGGGAGACCTGGATTGCTTGGGACCTGTTGGCCAGAATGCTGACTTTAGCACATACGGAATGGCTCCTACGGGGGATGACTACTTGGATTTCATGGAGTTGATTGATCTAGACTCCGAGATGTTCTGGCAGAGTGCTGCGGAACCTAAGTTGGACCTAAAATAA
Protein sequence

>Msa1193950

MNRSTETGFWKATGKDRAVQQNNQIVGMIKTLIFHTGKAPRGDRTDWVMHEYRLEDKDLTDRGIVQDSYVICKVFQKEGPGPRNGAQYGKPFNEEDWSDDEVGIPFAEPAALAPTLPVTSNSFVLNDQNLHAIGYNGSISMPCQSGLMPSPDPANSCQTGLQTGLPPSPDPANSCQTGVPPSPYPANSCQIGLTPSVDPVNSCQTGFMPSPDPSNSCQTGYMPSPDPANECQIGFMSPDPANSRQTGLELSPTGPANSCKTRLVPSPDPVDNSYHQAVNNDDPVDNSYHQAVNNDDILSMLDMFDDDILPEENNAEGALSTDFFDGLGDLDCLGPVGQNADFSTYGMAPTGDDYLDFMELIDLDSEMFWQSAAEPKLDLK*