AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1234980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234980 MtrunA17_Chr8g0337431 100.000 159 0 0 1 159 1 159 6.37e-119 332
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234980 AT3G03100.1 78.205 156 34 0 1 156 1 156 1.91e-93 268
Msa1234980 AT3G03100.2 77.564 156 35 0 1 156 1 156 1.42e-92 266

Find 68 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCTATGTTTGTATGTA+AGG 0.235909 8_2:+12780313 None:intergenic
AGACAAATTGACTGTGAAAT+TGG 0.292048 8_2:-12776970 Msa1234980:three_prime_UTR
TCAATCCAGGGCAGAGAAAC+TGG 0.299097 8_2:-12777171 Msa1234980:CDS
CAAACCAAGATCCACAATAT+TGG 0.303383 8_2:-12778808 Msa1234980:CDS
AAACCAAGATCCACAATATT+GGG 0.303795 8_2:-12778807 Msa1234980:CDS
TGCCCCAATATTGTGGATCT+TGG 0.327061 8_2:+12778804 None:intergenic
CCACAAACGAACCGTGAATA+TGG 0.337289 8_2:-12777126 Msa1234980:exon
CTGGTCCAGTTTCTCTGCCC+TGG 0.348930 8_2:+12777166 None:intergenic
TTCCTGCTGAATGGCATGGC+TGG 0.388219 8_2:-12777420 Msa1234980:CDS
AATTGAATTAACAATTTGTC+AGG 0.396851 8_2:+12777052 None:intergenic
GTGAAGATACATATGGGATA+TGG 0.399640 8_2:+12776770 None:intergenic
GTATACTCCAAATCAGAGAT+AGG 0.401046 8_2:+12776860 None:intergenic
AAAACTGAAAACACACAGTA+TGG 0.408491 8_2:-12778739 Msa1234980:intron
CCATATTCACGGTTCGTTTG+TGG 0.419524 8_2:+12777126 None:intergenic
AAGGGACATGCTCTCAATCC+AGG 0.420399 8_2:-12777184 Msa1234980:CDS
GCTTTGAGTTGCCGATGTAT+TGG 0.422998 8_2:-12777099 Msa1234980:three_prime_UTR
GTCTAGATATAATGCTTCTC+AGG 0.450196 8_2:-12777443 Msa1234980:CDS
ACAGGTATCCTTCATCTTTG+AGG 0.456990 8_2:+12780184 None:intergenic
TTTGTATGTAAGGCTGCGTT+TGG 0.463612 8_2:+12780323 None:intergenic
AAATAAAGTGAAGATACATA+TGG 0.469931 8_2:+12776763 None:intergenic
ACCGATTGGTGACAGTATAT+AGG 0.471413 8_2:+12776940 None:intergenic
CTTCTCAGGTTCCTGCTGAA+TGG 0.475351 8_2:-12777429 Msa1234980:CDS
AATATTGGGGCAACGCTTGT+TGG 0.475730 8_2:-12778793 Msa1234980:CDS
GATCATAAAGAAAATCTGTC+TGG 0.476518 8_2:-12777235 Msa1234980:CDS
CTTGTTGGTGTTGACAAATA+CGG 0.476795 8_2:-12778778 Msa1234980:CDS
AAGAGAAGAGAACGTGGTGG+TGG 0.480407 8_2:-12780293 Msa1234980:five_prime_UTR
TGTTAATTCAATTCATGAGG+TGG 0.505124 8_2:-12777042 Msa1234980:three_prime_UTR
TTAAACACGGTGAAGGAGAA+AGG 0.511115 8_2:-12780228 Msa1234980:CDS
GGACTGCCATGGTTGGTACC+TGG 0.513101 8_2:+12777147 None:intergenic
TCCTATATACTGTCACCAAT+CGG 0.521834 8_2:-12776941 Msa1234980:three_prime_UTR
CAGGTTCCTGCTGAATGGCA+TGG 0.525614 8_2:-12777424 Msa1234980:CDS
ACTTACAATAGGTTTCCATC+AGG 0.525711 8_2:+12779832 None:intergenic
TGGATTGAGAGCATGTCCCT+TGG 0.535784 8_2:+12777186 None:intergenic
TTCAGACACCTCAAAGATGA+AGG 0.536736 8_2:-12780192 Msa1234980:CDS
AGCCAGCCATGCCATTCAGC+AGG 0.538183 8_2:+12777418 None:intergenic
TGAATTAACAATTTGTCAGG+AGG 0.542153 8_2:+12777055 None:intergenic
AATAAAGTGAAGATACATAT+GGG 0.542755 8_2:+12776764 None:intergenic
ATCGATCATGGCGTCGTCGG+TGG 0.542913 8_2:-12780262 Msa1234980:exon
TCCAGGAGATGCCTTCCTGA+TGG 0.545756 8_2:-12779847 Msa1234980:intron
TCAAAGCTGAACCATATTCA+CGG 0.548554 8_2:+12777115 None:intergenic
GTGGATCGATCGATCGATCA+TGG 0.552835 8_2:-12780274 Msa1234980:exon
CAAGCGTTGCCCCAATATTG+TGG 0.553229 8_2:+12778797 None:intergenic
AATTGTTAATTCAATTCATG+AGG 0.553881 8_2:-12777045 Msa1234980:three_prime_UTR
AGAGTTTATCTATCATTCCA+AGG 0.554573 8_2:-12777203 Msa1234980:CDS
GGTTCGTTTGTGGACTGCCA+TGG 0.557488 8_2:+12777136 None:intergenic
CGTTTGTGGACTGCCATGGT+TGG 0.558192 8_2:+12777140 None:intergenic
TACATCACATACATACCGAT+TGG 0.564099 8_2:+12776926 None:intergenic
TATACTCCAAATCAGAGATA+GGG 0.566561 8_2:+12776861 None:intergenic
ACAATAGGTTTCCATCAGGA+AGG 0.596452 8_2:+12779836 None:intergenic
AAAATTTGGCAGGAAGACAC+AGG 0.598790 8_2:-12777486 Msa1234980:intron
ACGGTGAAGGAGAAAGGTCT+CGG 0.598993 8_2:-12780222 Msa1234980:CDS
GAGTTTATCTATCATTCCAA+GGG 0.600374 8_2:-12777202 Msa1234980:CDS
AGGGACATGCTCTCAATCCA+GGG 0.603322 8_2:-12777183 Msa1234980:CDS
AAAGAAAATCTGTCTGGACA+TGG 0.605520 8_2:-12777229 Msa1234980:CDS
TCGATCGATCATGGCGTCGT+CGG 0.616609 8_2:-12780265 Msa1234980:exon
GAGCATCTTAAACACGGTGA+AGG 0.622926 8_2:-12780235 Msa1234980:CDS
AAACATTCAATCCAATACAT+CGG 0.625421 8_2:+12777088 None:intergenic
AAGCGAATTTACCTCATCGC+CGG 0.625501 8_2:+12777375 None:intergenic
AACTGATCACACCGGCGATG+AGG 0.628022 8_2:-12777386 Msa1234980:intron
AACCAAGATCCACAATATTG+GGG 0.636179 8_2:-12778806 Msa1234980:CDS
TTTGGCAGGAAGACACAGGT+GGG 0.636329 8_2:-12777482 Msa1234980:intron
ATTTGGCAGGAAGACACAGG+TGG 0.636657 8_2:-12777483 Msa1234980:intron
ACTGGACCAGGTACCAACCA+TGG 0.665621 8_2:-12777153 Msa1234980:CDS
GAGAAGAGAAGAGAACGTGG+TGG 0.693087 8_2:-12780296 Msa1234980:five_prime_UTR
CACTTCATAACTGATCACAC+CGG 0.696701 8_2:-12777394 Msa1234980:CDS
ATAGAGAAGAGAAGAGAACG+TGG 0.711604 8_2:-12780299 Msa1234980:five_prime_UTR
GGTGAAGAGCATCTTAAACA+CGG 0.715006 8_2:-12780241 Msa1234980:CDS
GTGAAGGAGAAAGGTCTCGG+TGG 0.739929 8_2:-12780219 Msa1234980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAAAAAAAAAACTTATA+AGG - chr8_2:12778970-12778989 Msa1234980:intron 10.0%
!! AAAATAAAATGAAATAAGAA+TGG - chr8_2:12779104-12779123 Msa1234980:intron 10.0%
!! AAATAAAATGAAATAAGAAT+GGG - chr8_2:12779105-12779124 Msa1234980:intron 10.0%
!! TAAGAAGAATTTAATAATAG+TGG + chr8_2:12777105-12777124 None:intergenic 15.0%
!! TATGTTCAAAATAATGAATA+TGG - chr8_2:12777743-12777762 Msa1234980:intron 15.0%
!! ATGTTCAAAATAATGAATAT+GGG - chr8_2:12777744-12777763 Msa1234980:intron 15.0%
!! TAACTAACTAACTAATTAAA+GGG + chr8_2:12778620-12778639 None:intergenic 15.0%
!! ATAACTAACTAACTAATTAA+AGG + chr8_2:12778621-12778640 None:intergenic 15.0%
!!! ATTATTAGTTTTTATACTGA+GGG + chr8_2:12779064-12779083 None:intergenic 15.0%
!! TGACAATAAATTATAGAATA+TGG + chr8_2:12779564-12779583 None:intergenic 15.0%
!!! CTTTATATTTTTTTAGCATA+TGG + chr8_2:12779813-12779832 None:intergenic 15.0%
!! TCATAATTGAAAAAGAGAAA+TGG + chr8_2:12776995-12777014 None:intergenic 20.0%
!!! TCCAATTGATTTCAATTTTT+CGG - chr8_2:12777165-12777184 Msa1234980:CDS 20.0%
!!! TATTTTTGTTTTGAATTTCC+AGG - chr8_2:12777271-12777290 Msa1234980:CDS 20.0%
!! GAAAATAGATTATATCCTAT+GGG + chr8_2:12778020-12778039 None:intergenic 20.0%
!! TGAAAATAGATTATATCCTA+TGG + chr8_2:12778021-12778040 None:intergenic 20.0%
!! CTTTACAGAAACAAATTTAA+GGG + chr8_2:12778177-12778196 None:intergenic 20.0%
!! ACTTTACAGAAACAAATTTA+AGG + chr8_2:12778178-12778197 None:intergenic 20.0%
!!! TAAATTTGTTTCTGTAAAGT+TGG - chr8_2:12778178-12778197 Msa1234980:intron 20.0%
!!! CATTATTAGTTTTTATACTG+AGG + chr8_2:12779065-12779084 None:intergenic 20.0%
!! TAAATGTCTAATGCAATTAT+TGG - chr8_2:12779204-12779223 Msa1234980:intron 20.0%
!! ATATCACAATATTTACAATC+AGG - chr8_2:12779259-12779278 Msa1234980:intron 20.0%
!! GACACATTATTGTTAAAATT+TGG - chr8_2:12779635-12779654 Msa1234980:intron 20.0%
!! TATTACTTAGGAAAAGAATT+AGG + chr8_2:12779790-12779809 None:intergenic 20.0%
!! TTCATTGCAAATATTATGTT+GGG + chr8_2:12779837-12779856 None:intergenic 20.0%
!! ATTCATTGCAAATATTATGT+TGG + chr8_2:12779838-12779857 None:intergenic 20.0%
!!! TTCTTTTATTGAAACCAAAA+AGG - chr8_2:12779868-12779887 Msa1234980:intron 20.0%
!! AATTGAATTAACAATTTGTC+AGG + chr8_2:12780086-12780105 None:intergenic 20.0%
!! AATTGTTAATTCAATTCATG+AGG - chr8_2:12780090-12780109 Msa1234980:intron 20.0%
!!! CTGTATATAATTTTCATAAC+AGG + chr8_2:12780345-12780364 None:intergenic 20.0%
! TTGAAAAAGAGAAATGGAAA+GGG + chr8_2:12776989-12777008 None:intergenic 25.0%
! ATTGAAAAAGAGAAATGGAA+AGG + chr8_2:12776990-12777009 None:intergenic 25.0%
! TTAATAATCCCAAAAACGAA+AGG + chr8_2:12777042-12777061 None:intergenic 25.0%
!! CTTCTTATTTAACTGCAAAA+TGG - chr8_2:12777118-12777137 Msa1234980:exon 25.0%
! ACCGAAAAATTGAAATCAAT+TGG + chr8_2:12777169-12777188 None:intergenic 25.0%
!! AACAAAAATCGACTTACAAT+AGG + chr8_2:12777317-12777336 None:intergenic 25.0%
!!! TGTTGTTTTTGGATGTAAAT+CGG - chr8_2:12777481-12777500 Msa1234980:intron 25.0%
!! ACAATTTTTCTCTATTGTGA+TGG - chr8_2:12777662-12777681 Msa1234980:intron 25.0%
! CAAAATAATGAATATGGGAT+AGG - chr8_2:12777749-12777768 Msa1234980:intron 25.0%
!! GTTAAGTTGTTTATCCAAAT+AGG + chr8_2:12777939-12777958 None:intergenic 25.0%
! AAAGTAAAAAGATTTCCCAT+AGG - chr8_2:12778002-12778021 Msa1234980:intron 25.0%
!! TCTATTTTCAATAGCTATCT+TGG - chr8_2:12778031-12778050 Msa1234980:intron 25.0%
! ATTGCTTTCTGAATTTATGT+AGG - chr8_2:12778304-12778323 Msa1234980:intron 25.0%
! TTTATTCTGATAAATGGATG+TGG - chr8_2:12778462-12778481 Msa1234980:intron 25.0%
! TATGACATAATGAACATGAT+AGG - chr8_2:12778589-12778608 Msa1234980:intron 25.0%
! CTTATCATTTGAGAGAATTA+CGG - chr8_2:12778686-12778705 Msa1234980:intron 25.0%
! GACATAACTAACTAACTAAA+GGG + chr8_2:12778733-12778752 None:intergenic 25.0%
! TGACATAACTAACTAACTAA+AGG + chr8_2:12778625-12778644 None:intergenic 25.0%
!!! TATTCTAGTTCTCTAATAGA+AGG - chr8_2:12778862-12778881 Msa1234980:intron 25.0%
! TGACAAAATGTCATTCTTAT+AGG + chr8_2:12778953-12778972 None:intergenic 25.0%
! AAAAACTTATAAGGATGACA+TGG - chr8_2:12778979-12778998 Msa1234980:intron 25.0%
! AAAACTAATAATGCTAGCAT+TGG - chr8_2:12779072-12779091 Msa1234980:intron 25.0%
! AATGAAATAAGAATGGGATA+TGG - chr8_2:12779111-12779130 Msa1234980:intron 25.0%
! ATCTCATATGAGTTTCTATA+TGG - chr8_2:12779160-12779179 Msa1234980:intron 25.0%
!! ATATTGATTGCTTACTTGTA+AGG + chr8_2:12779243-12779262 None:intergenic 25.0%
! CATTATTGTTAAAATTTGGC+AGG - chr8_2:12779639-12779658 Msa1234980:intron 25.0%
! TTTAGCATATGGTATTACTT+AGG + chr8_2:12779802-12779821 None:intergenic 25.0%
! TCATTGCAAATATTATGTTG+GGG + chr8_2:12779836-12779855 None:intergenic 25.0%
! TTATTGAAACCAAAAAGGTA+CGG - chr8_2:12779873-12779892 Msa1234980:intron 25.0%
! AAACATTCAATCCAATACAT+CGG + chr8_2:12780050-12780069 None:intergenic 25.0%
!! AATTTTCATAACAGGTTTGA+GGG + chr8_2:12780337-12780356 None:intergenic 25.0%
!! TAATTTTCATAACAGGTTTG+AGG + chr8_2:12780338-12780357 None:intergenic 25.0%
CTTCTCTATGTTTGTATGTA+AGG + chr8_2:12776825-12776844 None:intergenic 30.0%
TGAAAAAGAGAAATGGAAAG+GGG + chr8_2:12776988-12777007 None:intergenic 30.0%
GGGCTGAATAATGATTATTA+TGG + chr8_2:12777066-12777085 None:intergenic 30.0%
! GGAGTTTGATTTCAACTATA+CGG + chr8_2:12777148-12777167 None:intergenic 30.0%
TGCTTCATAATTTCCCTAAA+TGG - chr8_2:12777435-12777454 Msa1234980:CDS 30.0%
AATTTCCCTAAATGGTTAGT+TGG - chr8_2:12777443-12777462 Msa1234980:CDS 30.0%
!!! GTTGTTTTTGGATGTAAATC+GGG - chr8_2:12777482-12777501 Msa1234980:intron 30.0%
! ATTTTTCTCTATTGTGATGG+AGG - chr8_2:12777665-12777684 Msa1234980:intron 30.0%
!! TTTTTCTCTATTGTGATGGA+GGG - chr8_2:12777666-12777685 Msa1234980:intron 30.0%
!! TTTTGAAGAAAAGGAATGTG+AGG - chr8_2:12777690-12777709 Msa1234980:intron 30.0%
TTTGAAGAAAAGGAATGTGA+GGG - chr8_2:12777691-12777710 Msa1234980:intron 30.0%
ATTGCAATCAGATTCCTATT+TGG - chr8_2:12777922-12777941 Msa1234980:intron 30.0%
! TGGATAAACAACTTAACTTG+AGG - chr8_2:12777942-12777961 Msa1234980:intron 30.0%
ACACAAGGAAATTCAAATCT+CGG + chr8_2:12778257-12778276 None:intergenic 30.0%
AATTCAGAAAGCAATTAGAG+TGG + chr8_2:12778299-12778318 None:intergenic 30.0%
AAACCAAGATCCACAATATT+GGG - chr8_2:12778328-12778347 Msa1234980:intron 30.0%
AAAACTGAAAACACACAGTA+TGG - chr8_2:12778396-12778415 Msa1234980:intron 30.0%
GTGTGGTTTATTCTGATAAA+TGG - chr8_2:12778456-12778475 Msa1234980:intron 30.0%
CTTATCATTCGAGAGAATTA+CGG - chr8_2:12778795-12778814 Msa1234980:CDS 30.0%
AGAATACTGTAGAACTTTGA+GGG + chr8_2:12778848-12778867 None:intergenic 30.0%
TAGAATACTGTAGAACTTTG+AGG + chr8_2:12778849-12778868 None:intergenic 30.0%
!! TTAGTTTTTATACTGAGGGT+GGG + chr8_2:12779060-12779079 None:intergenic 30.0%
!!! ATTAGTTTTTATACTGAGGG+TGG + chr8_2:12779061-12779080 None:intergenic 30.0%
AATCATCTTATAGGCATGTT+CGG + chr8_2:12779350-12779369 None:intergenic 30.0%
ATGGAATGCAATCATCTTAT+AGG + chr8_2:12779359-12779378 None:intergenic 30.0%
TAAGATGATTGCATTCCATT+TGG - chr8_2:12779360-12779379 Msa1234980:intron 30.0%
AAGATGATTGCATTCCATTT+GGG - chr8_2:12779361-12779380 Msa1234980:intron 30.0%
! AAGTGTTATTGCCATTGATT+GGG + chr8_2:12779494-12779513 None:intergenic 30.0%
! GAAAGTATGATTCACTTTAG+AGG - chr8_2:12779527-12779546 Msa1234980:intron 30.0%
ATTGTCAAGACTCAAAACTT+TGG - chr8_2:12779577-12779596 Msa1234980:intron 30.0%
GATCATAAAGAAAATCTGTC+TGG - chr8_2:12779900-12779919 Msa1234980:intron 30.0%
AGAGTTTATCTATCATTCCA+AGG - chr8_2:12779932-12779951 Msa1234980:intron 30.0%
GAGTTTATCTATCATTCCAA+GGG - chr8_2:12779933-12779952 Msa1234980:intron 30.0%
TGAATTAACAATTTGTCAGG+AGG + chr8_2:12780083-12780102 None:intergenic 30.0%
TGTTAATTCAATTCATGAGG+TGG - chr8_2:12780093-12780112 Msa1234980:intron 30.0%
AGACAAATTGACTGTGAAAT+TGG - chr8_2:12780165-12780184 Msa1234980:intron 30.0%
TATACTCCAAATCAGAGATA+GGG + chr8_2:12780277-12780296 None:intergenic 30.0%
! ATTTTCATAACAGGTTTGAG+GGG + chr8_2:12780336-12780355 None:intergenic 30.0%
!! CAAAAACGAAAGGTACTACA+CGG + chr8_2:12777032-12777051 None:intergenic 35.0%
!! GTGTAGTACCTTTCGTTTTT+GGG - chr8_2:12777031-12777050 Msa1234980:three_prime_UTR 35.0%
!!! GTGGAAAAAGCGCATTTTTT+GGG + chr8_2:12777086-12777105 None:intergenic 35.0%
!!! AGTGGAAAAAGCGCATTTTT+TGG + chr8_2:12777087-12777106 None:intergenic 35.0%
ACTTACAATAGGTTTCCATC+AGG + chr8_2:12777306-12777325 None:intergenic 35.0%
!!! GAGCTTTTAGGTGTTGTTTT+TGG - chr8_2:12777470-12777489 Msa1234980:CDS 35.0%
!! ATGGAGGGTTTTTGAAGAAA+AGG - chr8_2:12777681-12777700 Msa1234980:intron 35.0%
TTTCAACTCAACCTAAGGTA+TGG + chr8_2:12777887-12777906 None:intergenic 35.0%
AGTTCTTTCAACTCAACCTA+AGG + chr8_2:12777892-12777911 None:intergenic 35.0%
GGCTTATTGAGTTTATCTAC+TGG + chr8_2:12778142-12778161 None:intergenic 35.0%
AACAAATTTAAGGGCCTGTT+TGG + chr8_2:12778168-12778187 None:intergenic 35.0%
!! GTTTCTGTAAAGTTGGTGAA+AGG - chr8_2:12778185-12778204 Msa1234980:intron 35.0%
ACAAGGAAATTCAAATCTCG+GGG + chr8_2:12778255-12778274 None:intergenic 35.0%
CACAAGGAAATTCAAATCTC+GGG + chr8_2:12778256-12778275 None:intergenic 35.0%
CAAACCAAGATCCACAATAT+TGG - chr8_2:12778327-12778346 Msa1234980:intron 35.0%
AACCAAGATCCACAATATTG+GGG - chr8_2:12778329-12778348 Msa1234980:intron 35.0%
!! CTTGTTGGTGTTGACAAATA+CGG - chr8_2:12778357-12778376 Msa1234980:intron 35.0%
TCTATATCTGTATGTGTGTG+TGG - chr8_2:12778439-12778458 Msa1234980:intron 35.0%
TTTGAGAGAATTACGGCATT+TGG - chr8_2:12778693-12778712 Msa1234980:intron 35.0%
TTGAGAGAATTACGGCATTT+GGG - chr8_2:12778694-12778713 Msa1234980:intron 35.0%
AATTACGGCATTTGGGTAAT+TGG - chr8_2:12778701-12778720 Msa1234980:intron 35.0%
ATTACGGCATTTGGGTAATT+GGG - chr8_2:12778702-12778721 Msa1234980:intron 35.0%
AATTACGGCATCTAGGTAAT+TGG - chr8_2:12778810-12778829 Msa1234980:CDS 35.0%
ATTACGGCATCTAGGTAATT+GGG - chr8_2:12778811-12778830 Msa1234980:CDS 35.0%
TGTTTGGTAAGTAGGAAGTA+GGG + chr8_2:12778894-12778913 None:intergenic 35.0%
! CCTCTCTATCTTGTTATGAT+GGG + chr8_2:12778921-12778940 None:intergenic 35.0%
CCATCATAACAAGATAGAGA+GGG - chr8_2:12778919-12778938 Msa1234980:intron 35.0%
CATCATAACAAGATAGAGAG+GGG - chr8_2:12778920-12778939 Msa1234980:intron 35.0%
ATCATAACAAGATAGAGAGG+GGG - chr8_2:12778921-12778940 Msa1234980:intron 35.0%
AACTTATAAGGATGACATGG+TGG - chr8_2:12778982-12779001 Msa1234980:intron 35.0%
! TAGTTTTTATACTGAGGGTG+GGG + chr8_2:12779059-12779078 None:intergenic 35.0%
TATGGAGTAGAATACTACTG+AGG - chr8_2:12779178-12779197 Msa1234980:intron 35.0%
ATGGAGTAGAATACTACTGA+GGG - chr8_2:12779179-12779198 Msa1234980:intron 35.0%
! GAAGTGTTATTGCCATTGAT+TGG + chr8_2:12779495-12779514 None:intergenic 35.0%
TCAAGACTCAAAACTTTGGT+TGG - chr8_2:12779581-12779600 Msa1234980:intron 35.0%
GTCTAGATATAATGCTTCTC+AGG - chr8_2:12779692-12779711 Msa1234980:intron 35.0%
AAAGAAAATCTGTCTGGACA+TGG - chr8_2:12779906-12779925 Msa1234980:intron 35.0%
TCAAAGCTGAACCATATTCA+CGG + chr8_2:12780023-12780042 None:intergenic 35.0%
TCCTATATACTGTCACCAAT+CGG - chr8_2:12780194-12780213 Msa1234980:CDS 35.0%
TACATCACATACATACCGAT+TGG + chr8_2:12780212-12780231 None:intergenic 35.0%
! ATTTTGCCCTATCTCTGATT+TGG - chr8_2:12780268-12780287 Msa1234980:exon 35.0%
GTATACTCCAAATCAGAGAT+AGG + chr8_2:12780278-12780297 None:intergenic 35.0%
TTTGTATGTAAGGCTGCGTT+TGG + chr8_2:12776815-12776834 None:intergenic 40.0%
ATAGAGAAGAGAAGAGAACG+TGG - chr8_2:12776836-12776855 Msa1234980:three_prime_UTR 40.0%
!! GGTGAAGAGCATCTTAAACA+CGG - chr8_2:12776894-12776913 Msa1234980:three_prime_UTR 40.0%
TTAAACACGGTGAAGGAGAA+AGG - chr8_2:12776907-12776926 Msa1234980:three_prime_UTR 40.0%
TTCAGACACCTCAAAGATGA+AGG - chr8_2:12776943-12776962 Msa1234980:three_prime_UTR 40.0%
ACAGGTATCCTTCATCTTTG+AGG + chr8_2:12776954-12776973 None:intergenic 40.0%
GAGAAATGGAAAGGGGAAAA+GGG + chr8_2:12776981-12777000 None:intergenic 40.0%
AGAGAAATGGAAAGGGGAAA+AGG + chr8_2:12776982-12777001 None:intergenic 40.0%
! CGTGTAGTACCTTTCGTTTT+TGG - chr8_2:12777030-12777049 Msa1234980:three_prime_UTR 40.0%
ACAATAGGTTTCCATCAGGA+AGG + chr8_2:12777302-12777321 None:intergenic 40.0%
CGCATCCAACTAACCATTTA+GGG + chr8_2:12777451-12777470 None:intergenic 40.0%
TCGCATCCAACTAACCATTT+AGG + chr8_2:12777452-12777471 None:intergenic 40.0%
! TTAGTTGGATGCGAGCTTTT+AGG - chr8_2:12777458-12777477 Msa1234980:CDS 40.0%
! CGGGTTTGAAGAGAGTTTTT+AGG - chr8_2:12777609-12777628 Msa1234980:intron 40.0%
! TGAGGGCAGCATTTTCATTT+TGG - chr8_2:12777708-12777727 Msa1234980:intron 40.0%
ATGGTTGGATGGAGGATTTA+GGG + chr8_2:12777868-12777887 None:intergenic 40.0%
TATGGTTGGATGGAGGATTT+AGG + chr8_2:12777869-12777888 None:intergenic 40.0%
AACTCAACCTAAGGTATGGT+TGG + chr8_2:12777883-12777902 None:intergenic 40.0%
AAGCACTTGTGAGACTGTTT+GGG + chr8_2:12778116-12778135 None:intergenic 40.0%
TAAGCACTTGTGAGACTGTT+TGG + chr8_2:12778117-12778136 None:intergenic 40.0%
CTCAATAAGCCAATCCAAAC+AGG - chr8_2:12778151-12778170 Msa1234980:intron 40.0%
ATTTAAGGGCCTGTTTGGAT+TGG + chr8_2:12778163-12778182 None:intergenic 40.0%
GGAAAAACACGTGTAACACA+AGG + chr8_2:12778272-12778291 None:intergenic 40.0%
GAAAGCAATTAGAGTGGAAG+AGG + chr8_2:12778293-12778312 None:intergenic 40.0%
ACAGATATAGACAGAGAGAG+CGG + chr8_2:12778430-12778449 None:intergenic 40.0%
AAAACCAGAATGCTTGAAGC+TGG + chr8_2:12778551-12778570 None:intergenic 40.0%
! TAGGTAATTGGGTGGTTTGT+AGG - chr8_2:12778822-12778841 Msa1234980:intron 40.0%
GTTTGGTAAGTAGGAAGTAG+GGG + chr8_2:12778893-12778912 None:intergenic 40.0%
CTGTTTGGTAAGTAGGAAGT+AGG + chr8_2:12778895-12778914 None:intergenic 40.0%
CTACTTCCTACTTACCAAAC+AGG - chr8_2:12778893-12778912 Msa1234980:intron 40.0%
! TGTTATGATGGGAACCTGTT+TGG + chr8_2:12778910-12778929 None:intergenic 40.0%
CCCATCATAACAAGATAGAG+AGG - chr8_2:12778918-12778937 Msa1234980:intron 40.0%
! CCCTCTCTATCTTGTTATGA+TGG + chr8_2:12778922-12778941 None:intergenic 40.0%
TGGGATATGGTGTAATGTTG+TGG - chr8_2:12779124-12779143 Msa1234980:intron 40.0%
! CCATTGATTGGGAAATCAAC+GGG + chr8_2:12779483-12779502 None:intergenic 40.0%
! GCCATTGATTGGGAAATCAA+CGG + chr8_2:12779484-12779503 None:intergenic 40.0%
AAAATTTGGCAGGAAGACAC+AGG - chr8_2:12779649-12779668 Msa1234980:intron 40.0%
CACTTCATAACTGATCACAC+CGG - chr8_2:12779741-12779760 Msa1234980:intron 40.0%
! TGATCAAGACCGTACCTTTT+TGG + chr8_2:12779885-12779904 None:intergenic 40.0%
! ACCGATTGGTGACAGTATAT+AGG + chr8_2:12780198-12780217 None:intergenic 40.0%
GAGCATCTTAAACACGGTGA+AGG - chr8_2:12776900-12776919 Msa1234980:three_prime_UTR 45.0%
AAAGGGGAAAAGGGACAAAC+AGG + chr8_2:12776972-12776991 None:intergenic 45.0%
TGAGAGTTTGTCGCCCATTT+GGG + chr8_2:12777556-12777575 None:intergenic 45.0%
TGTCGTTGCATACCCAAACT+CGG - chr8_2:12777589-12777608 Msa1234980:intron 45.0%
CTCTCTTCAAACCCGAGTTT+GGG + chr8_2:12777604-12777623 None:intergenic 45.0%
ACTCTCTTCAAACCCGAGTT+TGG + chr8_2:12777605-12777624 None:intergenic 45.0%
TGGTTGGATGGAGGATTTAG+GGG + chr8_2:12777867-12777886 None:intergenic 45.0%
CAACCTAAGGTATGGTTGGA+TGG + chr8_2:12777879-12777898 None:intergenic 45.0%
TGCCCCAATATTGTGGATCT+TGG + chr8_2:12778334-12778353 None:intergenic 45.0%
AATATTGGGGCAACGCTTGT+TGG - chr8_2:12778342-12778361 Msa1234980:intron 45.0%
AGATATAGACAGAGAGAGCG+GGG + chr8_2:12778428-12778447 None:intergenic 45.0%
CAGATATAGACAGAGAGAGC+GGG + chr8_2:12778429-12778448 None:intergenic 45.0%
AAGACCAGTTGCGATCACTT+GGG - chr8_2:12778516-12778535 Msa1234980:intron 45.0%
TCGAGAGAATTACGGCATCT+AGG - chr8_2:12778803-12778822 Msa1234980:CDS 45.0%
TGGGAACCTGTTTGGTAAGT+AGG + chr8_2:12778902-12778921 None:intergenic 45.0%
!! TAAGGATGACATGGTGGATG+AGG - chr8_2:12778988-12779007 Msa1234980:intron 45.0%
AGTTAAGGAGTGGAGTGATG+AGG - chr8_2:12779017-12779036 Msa1234980:intron 45.0%
GCACACACAATTGTCCCAAA+TGG + chr8_2:12779378-12779397 None:intergenic 45.0%
!! CCCGTTGATTTCCCAATCAA+TGG - chr8_2:12779480-12779499 Msa1234980:intron 45.0%
AAGCGAATTTACCTCATCGC+CGG + chr8_2:12779763-12779782 None:intergenic 45.0%
CCATATTCACGGTTCGTTTG+TGG + chr8_2:12780012-12780031 None:intergenic 45.0%
CCACAAACGAACCGTGAATA+TGG - chr8_2:12780009-12780028 Msa1234980:intron 45.0%
!! GCTTTGAGTTGCCGATGTAT+TGG - chr8_2:12780036-12780055 Msa1234980:intron 45.0%
GAGAAGAGAAGAGAACGTGG+TGG - chr8_2:12776839-12776858 Msa1234980:three_prime_UTR 50.0%
AAGAGAAGAGAACGTGGTGG+TGG - chr8_2:12776842-12776861 Msa1234980:three_prime_UTR 50.0%
GTGGATCGATCGATCGATCA+TGG - chr8_2:12776861-12776880 Msa1234980:three_prime_UTR 50.0%
ACGGTGAAGGAGAAAGGTCT+CGG - chr8_2:12776913-12776932 Msa1234980:three_prime_UTR 50.0%
ACTAAACCCTCGCGCTTTGT+GGG - chr8_2:12777529-12777548 Msa1234980:intron 50.0%
GTGAGAGTTTGTCGCCCATT+TGG + chr8_2:12777557-12777576 None:intergenic 50.0%
GTCGTTGCATACCCAAACTC+GGG - chr8_2:12777590-12777609 Msa1234980:intron 50.0%
TTGGATGGAGGATTTAGGGG+GGG + chr8_2:12777864-12777883 None:intergenic 50.0%
GTTGGATGGAGGATTTAGGG+GGG + chr8_2:12777865-12777884 None:intergenic 50.0%
GGTTGGATGGAGGATTTAGG+GGG + chr8_2:12777866-12777885 None:intergenic 50.0%
CCTAAGGTATGGTTGGATGG+AGG + chr8_2:12777876-12777895 None:intergenic 50.0%
CCTCCATCCAACCATACCTT+AGG - chr8_2:12777873-12777892 Msa1234980:intron 50.0%
! CAAGCGTTGCCCCAATATTG+TGG + chr8_2:12778341-12778360 None:intergenic 50.0%
! CAAGTGATCGCAACTGGTCT+TGG + chr8_2:12778517-12778536 None:intergenic 50.0%
CAAGACCAGTTGCGATCACT+TGG - chr8_2:12778515-12778534 Msa1234980:intron 50.0%
ATCGCCAGCTTCAAGCATTC+TGG - chr8_2:12778544-12778563 Msa1234980:intron 50.0%
ACGGCATCTAGGTAATTGGG+TGG - chr8_2:12778814-12778833 Msa1234980:intron 50.0%
!! GATGACATGGTGGATGAGGT+GGG - chr8_2:12778992-12779011 Msa1234980:intron 50.0%
TGGATGAGGTGGGAGAGTTA+AGG - chr8_2:12779002-12779021 Msa1234980:intron 50.0%
TAAGGAGTGGAGTGATGAGG+AGG - chr8_2:12779020-12779039 Msa1234980:intron 50.0%
TGGAGTGATGAGGAGGTCAA+TGG - chr8_2:12779027-12779046 Msa1234980:intron 50.0%
TGTGTGCAACACACAGCGAA+AGG - chr8_2:12779391-12779410 Msa1234980:intron 50.0%
ATTTGGCAGGAAGACACAGG+TGG - chr8_2:12779652-12779671 Msa1234980:intron 50.0%
TTTGGCAGGAAGACACAGGT+GGG - chr8_2:12779653-12779672 Msa1234980:intron 50.0%
CTTCTCAGGTTCCTGCTGAA+TGG - chr8_2:12779706-12779725 Msa1234980:intron 50.0%
! TGGATTGAGAGCATGTCCCT+TGG + chr8_2:12779952-12779971 None:intergenic 50.0%
AAGGGACATGCTCTCAATCC+AGG - chr8_2:12779951-12779970 Msa1234980:intron 50.0%
AGGGACATGCTCTCAATCCA+GGG - chr8_2:12779952-12779971 Msa1234980:intron 50.0%
TCAATCCAGGGCAGAGAAAC+TGG - chr8_2:12779964-12779983 Msa1234980:intron 50.0%
TCGATCGATCATGGCGTCGT+CGG - chr8_2:12776870-12776889 Msa1234980:three_prime_UTR 55.0%
GTGAAGGAGAAAGGTCTCGG+TGG - chr8_2:12776916-12776935 Msa1234980:three_prime_UTR 55.0%
TCCAGGAGATGCCTTCCTGA+TGG - chr8_2:12777288-12777307 Msa1234980:intron 55.0%
!! TCCATCAGGAAGGCATCTCC+TGG + chr8_2:12777292-12777311 None:intergenic 55.0%
GACTAAACCCTCGCGCTTTG+TGG - chr8_2:12777528-12777547 Msa1234980:intron 55.0%
GCGCTTTGTGGGACCCAAAT+GGG - chr8_2:12777540-12777559 Msa1234980:intron 55.0%
TTTAGGGGGGGACGAAAGGA+CGG + chr8_2:12777852-12777871 None:intergenic 55.0%
AGGATTTAGGGGGGGACGAA+AGG + chr8_2:12777856-12777875 None:intergenic 55.0%
!! CTGGTCTTGGCTCAAGGGAT+TGG + chr8_2:12778504-12778523 None:intergenic 55.0%
!! CGCAACTGGTCTTGGCTCAA+GGG + chr8_2:12778509-12778528 None:intergenic 55.0%
!! TCGCAACTGGTCTTGGCTCA+AGG + chr8_2:12778510-12778529 None:intergenic 55.0%
!! GGATGACATGGTGGATGAGG+TGG - chr8_2:12778991-12779010 Msa1234980:intron 55.0%
GAGGTGGGAGAGTTAAGGAG+TGG - chr8_2:12779007-12779026 Msa1234980:intron 55.0%
CAGGTTCCTGCTGAATGGCA+TGG - chr8_2:12779711-12779730 Msa1234980:intron 55.0%
TTCCTGCTGAATGGCATGGC+TGG - chr8_2:12779715-12779734 Msa1234980:intron 55.0%
AACTGATCACACCGGCGATG+AGG - chr8_2:12779749-12779768 Msa1234980:intron 55.0%
ACTGGACCAGGTACCAACCA+TGG - chr8_2:12779982-12780001 Msa1234980:intron 55.0%
! CGTTTGTGGACTGCCATGGT+TGG + chr8_2:12779998-12780017 None:intergenic 55.0%
! GGTTCGTTTGTGGACTGCCA+TGG + chr8_2:12780002-12780021 None:intergenic 55.0%
ATCGATCATGGCGTCGTCGG+TGG - chr8_2:12776873-12776892 Msa1234980:three_prime_UTR 60.0%
CGGGTATCCTCTGCCTGCAA+CGG - chr8_2:12777501-12777520 Msa1234980:intron 60.0%
GTATCCTCTGCCTGCAACGG+CGG - chr8_2:12777504-12777523 Msa1234980:intron 60.0%
GGTTTAGTCTCCGCCGTTGC+AGG + chr8_2:12777517-12777536 None:intergenic 60.0%
TTGGGTCCCACAAAGCGCGA+GGG + chr8_2:12777538-12777557 None:intergenic 60.0%
TTTGGGTCCCACAAAGCGCG+AGG + chr8_2:12777539-12777558 None:intergenic 60.0%
CGCGCTTTGTGGGACCCAAA+TGG - chr8_2:12777539-12777558 Msa1234980:intron 60.0%
GCAGCCCAAGTGATCGCAAC+TGG + chr8_2:12778523-12778542 None:intergenic 60.0%
AGCCAGCCATGCCATTCAGC+AGG + chr8_2:12779720-12779739 None:intergenic 60.0%
CTGGTCCAGTTTCTCTGCCC+TGG + chr8_2:12779972-12779991 None:intergenic 60.0%
CAGGGCAGAGAAACTGGACC+AGG - chr8_2:12779970-12779989 Msa1234980:intron 60.0%
! GGACTGCCATGGTTGGTACC+TGG + chr8_2:12779991-12780010 None:intergenic 60.0%
GTCTCCGCCGTTGCAGGCAG+AGG + chr8_2:12777511-12777530 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 12776773 12780384 12776773 ID=Msa1234980;Name=Msa1234980
chr8_2 mRNA 12776773 12780384 12776773 ID=Msa1234980-mRNA-1;Parent=Msa1234980;Name=Msa1234980-mRNA-1;_AED=0.05;_eAED=0.05;_QI=107|1|1|1|1|1|5|359|159
chr8_2 exon 12776773 12777290 12776773 ID=Msa1234980-mRNA-1:exon:4980;Parent=Msa1234980-mRNA-1
chr8_2 exon 12777387 12777496 12777387 ID=Msa1234980-mRNA-1:exon:4979;Parent=Msa1234980-mRNA-1
chr8_2 exon 12778740 12778831 12778740 ID=Msa1234980-mRNA-1:exon:4978;Parent=Msa1234980-mRNA-1
chr8_2 exon 12779838 12779864 12779838 ID=Msa1234980-mRNA-1:exon:4977;Parent=Msa1234980-mRNA-1
chr8_2 exon 12780186 12780384 12780186 ID=Msa1234980-mRNA-1:exon:4976;Parent=Msa1234980-mRNA-1
chr8_2 five_prime_UTR 12780278 12780384 12780278 ID=Msa1234980-mRNA-1:five_prime_utr;Parent=Msa1234980-mRNA-1
chr8_2 CDS 12780186 12780277 12780186 ID=Msa1234980-mRNA-1:cds;Parent=Msa1234980-mRNA-1
chr8_2 CDS 12779838 12779864 12779838 ID=Msa1234980-mRNA-1:cds;Parent=Msa1234980-mRNA-1
chr8_2 CDS 12778740 12778831 12778740 ID=Msa1234980-mRNA-1:cds;Parent=Msa1234980-mRNA-1
chr8_2 CDS 12777387 12777496 12777387 ID=Msa1234980-mRNA-1:cds;Parent=Msa1234980-mRNA-1
chr8_2 CDS 12777132 12777290 12777132 ID=Msa1234980-mRNA-1:cds;Parent=Msa1234980-mRNA-1
chr8_2 three_prime_UTR 12776773 12777131 12776773 ID=Msa1234980-mRNA-1:three_prime_utr;Parent=Msa1234980-mRNA-1
Gene Sequence

>Msa1234980

ATGGCGTCGTCGGTGGTGAAGAGCATCTTAAACACGGTGAAGGAGAAAGGTCTCGGTGGCTTCTTCAGACACCTCAAAGATGAAGGATACCTGAGATGCCTTCCTGATGGAAACCTATTGCAAACCAAGATCCACAATATTGGGGCAACGCTTGTTGGTGTTGACAAATACGGTAACAAGTATTATGAGAAAACTGAAAACACACAGTATGGAAGACACAGGTGGGTTGAATATGCAGAGAAGTCTAGATATAATGCTTCTCAGGTTCCTGCTGAATGGCATGGCTGGCTTCACTTCATAACTGATCACACCGGCGATGAGCTTCTTTTATTGAAACCAAAAAGGTACGGTCTTGATCATAAAGAAAATCTGTCTGGACATGGTGAAGAGTTTATCTATCATTCCAAGGGACATGCTCTCAATCCAGGGCAGAGAAACTGGACCAGGTACCAACCATGGCAGTCCACAAACGAACCGTGA

Protein sequence

>Msa1234980

MASSVVKSILNTVKEKGLGGFFRHLKDEGYLRCLPDGNLLQTKIHNIGATLVGVDKYGNKYYEKTENTQYGRHRWVEYAEKSRYNASQVPAEWHGWLHFITDHTGDELLLLKPKRYGLDHKENLSGHGEEFIYHSKGHALNPGQRNWTRYQPWQSTNEP*